Lsi11G007880 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi11G007880 vs. ExPASy Swiss-Prot
Match: O64481 (Subtilisin-like protease SBT2.5 OS=Arabidopsis thaliana OX=3702 GN=SBT2.5 PE=2 SV=1)

HSP 1 Score: 1345.9 bits (3482), Expect = 0.0e+00
Identity = 662/809 (81.83%), Postives = 729/809 (90.11%), Query Frame = 0

Query: 28  FLVFLAILVVGKAEIYIVTIEGEPIVSYKGDLDGFEATAMESDEKIDPTSEIVTSYARHL 87
           F+VF+ +LV   AE+YIVT+EG+PI+SYKG  +GFEATA+ESDEKID +SE+VT YARHL
Sbjct: 8   FVVFV-LLVAVTAEVYIVTMEGDPIISYKGGENGFEATAVESDEKIDTSSELVTVYARHL 67

Query: 88  ENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITHEQAEILRHTPIVKSVERDWKVRKL 147
           E KHDM+LGMLFE GS+KKLYSYKHLINGFA  ++ EQAE LR  P V+SV++DWKVR+L
Sbjct: 68  ERKHDMILGMLFEEGSYKKLYSYKHLINGFAAHVSPEQAETLRRAPGVRSVDKDWKVRRL 127

Query: 148 TTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGISPHHPSFATYNTEPFGPCLKYKG 207
           TTHTPEFLGLPT VWPTGGGFDRAGEDIVIGFVDSGI PHHPSFA+++  P+GP   YKG
Sbjct: 128 TTHTPEFLGLPTDVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFASHHRLPYGPLPHYKG 187

Query: 208 KCEIDPDTKKDFCNGKIVGAQHFAEAAKAAGAFNPAIHFASPLDGDGHGSHTAAIAAGNN 267
           KCE DP TKK FCN KIVGAQHFAEAAKAAGAFNP I +ASP+DGDGHGSHTAAIAAGNN
Sbjct: 188 KCEEDPHTKKSFCNRKIVGAQHFAEAAKAAGAFNPDIDYASPMDGDGHGSHTAAIAAGNN 247

Query: 268 GIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGVDILSLSVG 327
           GIP+RMHGYEFGKASGMAPRARIAVYKALYR+FGGFVADVVAAIDQAVHDGVDILSLSVG
Sbjct: 248 GIPLRMHGYEFGKASGMAPRARIAVYKALYRLFGGFVADVVAAIDQAVHDGVDILSLSVG 307

Query: 328 PNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIATVAAAIDD 387
           PNSPP TTK T+LNPFDATLL AVKAGVFVAQAAGNGGPFPKTLVSYSPWI TVAAAIDD
Sbjct: 308 PNSPPTTTKTTFLNPFDATLLGAVKAGVFVAQAAGNGGPFPKTLVSYSPWITTVAAAIDD 367

Query: 388 RRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVTKYSPSDCQKPEVLNK 447
           RRYKNHLTLGNGK+LAG+GLSP T  +R YTLV+ANDVLLDSSV+KY+PSDCQ+PEV NK
Sbjct: 368 RRYKNHLTLGNGKMLAGMGLSPPTRPHRLYTLVSANDVLLDSSVSKYNPSDCQRPEVFNK 427

Query: 448 RLVEGKVLLCGYSFSFVVGTASIKKVSQTAKSLGAAGFVLAVENISPGAKFDPVPVGIPG 507
           +LVEG +LLCGYSF+FVVGTASIKKV  TAK LGAAGFVL VEN+SPG KFDPVP  IPG
Sbjct: 428 KLVEGNILLCGYSFNFVVGTASIKKVVATAKHLGAAGFVLVVENVSPGTKFDPVPSAIPG 487

Query: 508 ILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEVALFSARGP 567
           ILITDVSKSMDLIDYYN ST RDWTGRVKSF A GSIGDGL P+L+KSAP+VALFSARGP
Sbjct: 488 ILITDVSKSMDLIDYYNASTSRDWTGRVKSFKAEGSIGDGLAPVLHKSAPQVALFSARGP 547

Query: 568 NIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMAAPHIAGIA 627
           N +DFSFQDADLLKPDILAPG LIWAAW PNGTDEPNYVGEGFA+ISGTSMAAPHIAGIA
Sbjct: 548 NTKDFSFQDADLLKPDILAPGYLIWAAWCPNGTDEPNYVGEGFALISGTSMAAPHIAGIA 607

Query: 628 ALVKQKHPNWSPAAIKSALMTTSTTMDRGGRPLKAQQFSETEAMKLVSATPFDYGSGHVN 687
           ALVKQKHP WSPAAIKSALMTTST +DR GR L+AQQ+S+TEA+ LV ATPFDYGSGHVN
Sbjct: 608 ALVKQKHPQWSPAAIKSALMTTSTVIDRAGRLLQAQQYSDTEAVTLVKATPFDYGSGHVN 667

Query: 688 PRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTNSPCNFTMGHPWNLNSPSITIAH 747
           P AALDPGLIFDAGYEDYLGFLCTT GI+ HEI NYTN+ CN+ M HP N N+PSI ++H
Sbjct: 668 PSAALDPGLIFDAGYEDYLGFLCTTPGISAHEIRNYTNTACNYDMKHPSNFNAPSIAVSH 727

Query: 748 LVGSQTVTRTVINVAE-EETYTITARMDPAVAIEVNPPAMTLRSGSSRKFSVTLTARSLT 807
           LVG+QTVTR V NVAE EETYTITARM P++AIEVNPPAMTLR G++R FSVT+T RS++
Sbjct: 728 LVGTQTVTRKVTNVAEVEETYTITARMQPSIAIEVNPPAMTLRPGATRTFSVTMTVRSVS 787

Query: 808 GTYSFGQVLLKGSRGHKVRIPVVAMGYQR 836
           G YSFG+V LKGSRGHKVRIPVVA+G++R
Sbjct: 788 GVYSFGEVKLKGSRGHKVRIPVVALGHRR 815

BLAST of Lsi11G007880 vs. ExPASy Swiss-Prot
Match: Q9SZV5 (Subtilisin-like protease SBT2.6 OS=Arabidopsis thaliana OX=3702 GN=SBT2.6 PE=2 SV=1)

HSP 1 Score: 1338.6 bits (3463), Expect = 0.0e+00
Identity = 660/809 (81.58%), Postives = 726/809 (89.74%), Query Frame = 0

Query: 29  LVFLAILVVGKAEIYIVTIEGEPIVSYKGDLDGFEATAMESDEKIDPTSEIVTSYARHLE 88
           LVF    +   AEIYIVT+EGEPI+SYKG  +GFEATA+ESDEKID TSE+VTSYARHLE
Sbjct: 8   LVFFTCFLTVTAEIYIVTMEGEPIISYKGGDNGFEATAVESDEKIDTTSELVTSYARHLE 67

Query: 89  NKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITHEQAEILRHTPIVKSVERDWKVRKLT 148
            KHDMLLGMLF  GS+KKLYSYKHLINGFA  ++ +QAE+LR  P VKSV+RDWKVRKLT
Sbjct: 68  RKHDMLLGMLFVEGSYKKLYSYKHLINGFAAHVSPDQAEMLRRAPGVKSVDRDWKVRKLT 127

Query: 149 THTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGISPHHPSFATYNTE-PFGPCLKYKG 208
           THTP+FLGLPT VWPTGGG+DRAGEDIVIGF+DSGI PHHPSFA+++T  P+GP   YKG
Sbjct: 128 THTPQFLGLPTDVWPTGGGYDRAGEDIVIGFIDSGIFPHHPSFASHHTTVPYGPHPSYKG 187

Query: 209 KCEIDPDTKKDFCNGKIVGAQHFAEAAKAAGAFNPAIHFASPLDGDGHGSHTAAIAAGNN 268
           KCE DP TK  FCNGKI+GAQHFAEAAKAAGAFNP I FASP+DGDGHGSHTAAIAAGNN
Sbjct: 188 KCEEDPHTKISFCNGKIIGAQHFAEAAKAAGAFNPDIDFASPMDGDGHGSHTAAIAAGNN 247

Query: 269 GIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGVDILSLSVG 328
           GIPVRMHGYEFGKASGMAPRARIAVYKALYR+FGGFVADVVAAIDQAVHDGVDILSLSVG
Sbjct: 248 GIPVRMHGYEFGKASGMAPRARIAVYKALYRLFGGFVADVVAAIDQAVHDGVDILSLSVG 307

Query: 329 PNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIATVAAAIDD 388
           PNSPPATTK T+LNPFDATLL AVKAGVFVAQAAGNGGPFPKTLVSYSPWI TVAAAIDD
Sbjct: 308 PNSPPATTKTTFLNPFDATLLGAVKAGVFVAQAAGNGGPFPKTLVSYSPWITTVAAAIDD 367

Query: 389 RRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVTKYSPSDCQKPEVLNK 448
           RRYKNHLTLGNGK+LAG+GLSP+T  +R+Y +V+ANDVLL SS  KY+PSDCQKPEVLNK
Sbjct: 368 RRYKNHLTLGNGKMLAGIGLSPSTRPHRSYKMVSANDVLLGSSGMKYNPSDCQKPEVLNK 427

Query: 449 RLVEGKVLLCGYSFSFVVGTASIKKVSQTAKSLGAAGFVLAVENISPGAKFDPVPVGIPG 508
           +LVEG +LLCGYSF+FV G+ASIKKV++TAK LGAAGFVL VEN+SPG KFDPVP  IPG
Sbjct: 428 KLVEGNILLCGYSFNFVAGSASIKKVAETAKHLGAAGFVLVVENVSPGTKFDPVPSCIPG 487

Query: 509 ILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEVALFSARGP 568
           ILITDVSKSMDLIDYYN +T RDW GRVK F A GSIGDGL P+L+KSAPEVALFSARGP
Sbjct: 488 ILITDVSKSMDLIDYYNVTTSRDWMGRVKDFKAEGSIGDGLEPILHKSAPEVALFSARGP 547

Query: 569 NIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMAAPHIAGIA 628
           N +DFSFQDADLLKPDILAPGSLIW+AWS NGTDE NY+GEGFA+ISGTSMAAPHIAGIA
Sbjct: 548 NTKDFSFQDADLLKPDILAPGSLIWSAWSANGTDEANYIGEGFALISGTSMAAPHIAGIA 607

Query: 629 ALVKQKHPNWSPAAIKSALMTTSTTMDRGGRPLKAQQFSETEAMKLVSATPFDYGSGHVN 688
           ALVKQKHP WSPAAIKSALMTTST +DR GRPL+AQQ+SETE + LV ATPFDYGSGHVN
Sbjct: 608 ALVKQKHPQWSPAAIKSALMTTSTVIDRAGRPLQAQQYSETETVTLVKATPFDYGSGHVN 667

Query: 689 PRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTNSPCNFTMGHPWNLNSPSITIAH 748
           P AALDPGLIFDAGYEDY+GFLCTT GI+ HEI N+TN+PCNF M HP N N+PSI I+H
Sbjct: 668 PSAALDPGLIFDAGYEDYIGFLCTTPGIDAHEIKNFTNTPCNFKMVHPSNFNTPSIAISH 727

Query: 749 LVGSQTVTRTVINVA-EEETYTITARMDPAVAIEVNPPAMTLRSGSSRKFSVTLTARSLT 808
           LV +QTVTR V NVA EEETYTIT+RM+PA+AIEV+PPAMT+R+G+SR FSVTLT RS+T
Sbjct: 728 LVRTQTVTRRVTNVAEEEETYTITSRMEPAIAIEVSPPAMTVRAGASRTFSVTLTVRSVT 787

Query: 809 GTYSFGQVLLKGSRGHKVRIPVVAMGYQR 836
           G YSFGQV LKGSRGHKV +PVVAMG +R
Sbjct: 788 GAYSFGQVTLKGSRGHKVTLPVVAMGQRR 816

BLAST of Lsi11G007880 vs. ExPASy Swiss-Prot
Match: Q9FI12 (Subtilisin-like protease SBT2.3 OS=Arabidopsis thaliana OX=3702 GN=SBT2.3 PE=2 SV=1)

HSP 1 Score: 661.0 bits (1704), Expect = 1.8e-188
Identity = 377/841 (44.83%), Postives = 519/841 (61.71%), Query Frame = 0

Query: 17  LETMVVNFQHTFL----VFLAILVVGKAE-------IYIVTIEGEPIVSYKGDLDGFEAT 76
           +  M+V F    L    VFL+   +G+ +       +YIVT++  PIV    + +  +  
Sbjct: 2   VRVMLVRFGFLLLMISFVFLSNNTLGQQQDDDDDSAVYIVTLKQPPIVHLFEEQE-LKHK 61

Query: 77  AMESDEKIDPTSEIVTSYARHLENK-------HDMLLGMLFERGSFKKLYSYKHLINGFA 136
             +   K+ P +    S  RH ++K       HD  L    +   + KLYSY +LINGFA
Sbjct: 62  KSKFTPKLRPRN---NSRKRHGKSKIPSVVQSHDSFLRKTLKGEKYIKLYSYHYLINGFA 121

Query: 137 VDITHEQAEILRHTPIVKSVERDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIG 196
           + I  +QAE L     V ++  D+ VR  TT+TP+F+GLP G W   GGF+ AGE ++IG
Sbjct: 122 LFINSQQAEKLSMRKEVANIVLDYSVRTATTYTPQFMGLPQGAWVKEGGFEIAGEGVIIG 181

Query: 197 FVDSGISPHHPSFATYNTEPFGPCLK-YKGKCEIDPDTKKDFCNGKIVGAQHFAEAAKAA 256
           F+D+GI P+HPSF   +++   P  K + G CE+ PD     CN K++GA+HFA++A   
Sbjct: 182 FIDTGIDPNHPSFNDNDSKRSYPIPKHFSGVCEVTPDFPSGSCNKKLIGARHFAQSAVTR 241

Query: 257 GAFNPAIHFASPLDGDGHGSHTAAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALY 316
           G FN +  +ASP DGDGHG+HTA++AAGN+G+PV +  + FG ASG+APRA I+VYKALY
Sbjct: 242 GIFNSSEDYASPFDGDGHGTHTASVAAGNHGVPVIVSNHNFGYASGIAPRAFISVYKALY 301

Query: 317 RIFGGFVADVVAAIDQAVHDGVDILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFV 376
           + FGGF ADVVAAIDQA  DGVDILSLS+ PN  P     T+ NP D  LLSAVKAG+FV
Sbjct: 302 KSFGGFAADVVAAIDQAAQDGVDILSLSITPNRKPPGV-ATFFNPIDMALLSAVKAGIFV 361

Query: 377 AQAAGNGGPFPKTLVSYSPWIATVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTY 436
            QAAGN GP PKT+ S+SPWI TV A+  DR Y N LTLGN   + G+G +  T   + Y
Sbjct: 362 VQAAGNTGPAPKTMSSFSPWIFTVGASSHDRVYSNSLTLGNNVTIPGMGFAIPTDSGKMY 421

Query: 437 TLVAANDVLLDS-SVTK-YSPSDCQKPEVLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQ 496
            +++A   L +S SV K     +CQ  E  ++  V GK+L+C YS  FV+G ++IK+   
Sbjct: 422 KMISAFHALNNSTSVDKDMYVGECQDYENFDQDRVSGKLLICSYSARFVLGLSTIKQALD 481

Query: 497 TAKSLGAAGFVLAVENISPGAKFDPVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGR- 556
            AK+L A G +  ++    G + +P P+ +PGI+I  V  S  L+ YYN+S  RD T + 
Sbjct: 482 VAKNLSATGVIFYIDPYVLGFEINPTPMDMPGIIIPSVEDSKTLLKYYNSSIQRDVTTKE 541

Query: 557 VKSFDAVGSIGDGLMPLLYKSAPEVALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAA 616
           + SF AV +I  GL       AP+V  +SARGP+  D SF DAD+LKP+++APG+ IW A
Sbjct: 542 IVSFGAVAAIEGGLNANFSNRAPKVMYYSARGPDPEDNSFNDADVLKPNLVAPGNSIWGA 601

Query: 617 WSPNGTDEPNYVGEGFAMISGTSMAAPHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMD 676
           WS   TD   + GE FAM+SGTSMAAPH+AG+AAL+KQ +P ++P+ I SAL TT+   D
Sbjct: 602 WSSASTDSTEFEGEKFAMMSGTSMAAPHVAGVAALIKQSYPQFTPSTISSALSTTALLND 661

Query: 677 RGGRPLKAQQFSETEAMKLVSATPFDYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAG 736
             G P+ AQ+        L +ATP D GSG VN  AALDPGL+FD  +EDY+ FLC   G
Sbjct: 662 NKGSPIMAQRTYSNPDQSLYTATPSDMGSGFVNATAALDPGLVFDTSFEDYISFLC---G 721

Query: 737 INVHE--IHNYT--NSPCNFTMGHPWNLNSPSITIAHLVGSQTVTRTVINVAEEETYTIT 796
           IN  +  + NYT    P N T    ++LN PSIT++ L G+QT  R++ N+A  ETY + 
Sbjct: 722 INGSDTVVFNYTGFRCPANNTPVSGFDLNLPSITVSTLSGTQTFQRSMRNIAGNETYNVG 781

Query: 797 ARMDPAVAIEVNPPAMTLRSGSSRKFSVTLTARSLTGTYSFGQVLLKGSRGHKVRIPVVA 832
                 V+++V+P   ++  G ++  SVTLT    + + SFG++ L G+ GH V IPV  
Sbjct: 782 WSPPYGVSMKVSPTQFSIAMGENQVLSVTLTVTKNSSSSSFGRIGLFGNTGHIVNIPVTV 834

BLAST of Lsi11G007880 vs. ExPASy Swiss-Prot
Match: Q9SUN6 (Subtilisin-like protease SBT2.2 OS=Arabidopsis thaliana OX=3702 GN=SBT2.2 PE=3 SV=1)

HSP 1 Score: 647.9 bits (1670), Expect = 1.6e-184
Identity = 351/747 (46.99%), Postives = 471/747 (63.05%), Query Frame = 0

Query: 91  HDMLLGMLFERGSFKKLYSYKHLINGFAVDITHEQAEILRHTPIVKSVERDWKVRKLTTH 150
           HD LL    +   + KLYS+ +LINGFAV ++ +QAE L     V ++  D+ VR  TT+
Sbjct: 103 HDSLLRNALKGEKYIKLYSFHYLINGFAVFVSSQQAETLSRRREVANIVLDFSVRTATTY 162

Query: 151 TPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGISPHHPSFATYNT--EPFGPCLKYKGK 210
           TP+F+GLP G W   GG++ AGE IVIGF+D+GI P HPSF   +T    +     + G 
Sbjct: 163 TPQFMGLPKGAWVKEGGYETAGEGIVIGFIDTGIDPTHPSFNGTDTSQRQYPIPNHFSGV 222

Query: 211 CEIDPDTKKDFCNGKIVGAQHFAEAAKAAGAFNPAIHFASPLDGDGHGSHTAAIAAGNNG 270
           CE+ PD     CN K+VGA+HFA++A   G FN +  +ASP DGDGHG+HTA+IAAGN+G
Sbjct: 223 CEVTPDFPSGSCNRKLVGARHFAQSAITRGIFNSSEDYASPFDGDGHGTHTASIAAGNHG 282

Query: 271 IPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGVDILSLSVGP 330
           +   + G+ FG ASG+APRA I+VYKALY+ FGGF ADVVAAIDQA  DGVDILSLS+ P
Sbjct: 283 VSAVVSGHNFGSASGIAPRAHISVYKALYKSFGGFAADVVAAIDQAAQDGVDILSLSITP 342

Query: 331 NSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIATVAAAIDDR 390
           N  P     T+ NP D  +LSAVKAG+FV QAAGN GP PK++ S+SPWI TV AA  DR
Sbjct: 343 NRRPPGV-ATFFNPLDMAMLSAVKAGIFVVQAAGNTGPSPKSMSSFSPWIFTVGAASHDR 402

Query: 391 RYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVT---KYSPSDCQKPEVL 450
            Y N + LGN   + G+GL+  T   + YT+++A D L + S          +CQ     
Sbjct: 403 DYSNSIVLGNNVSIPGVGLALRTDEGKKYTMISALDALKNKSSVVDKDMYVGECQDYGSF 462

Query: 451 NKRLVEGKVLLCGYSFSFVVGTASIKKVSQTAKSLGAAGFVLAVENISPGAKFDPVPVGI 510
           +K ++ G +L+C YS  FV+G ++IK+    AK+L A G V  ++    G + +P P+ +
Sbjct: 463 DKDVIRGNLLICSYSIRFVLGLSTIKQALAVAKNLSAKGVVFYMDPYVLGFQINPTPMDM 522

Query: 511 PGILITDVSKSMDLIDYYNTSTPRDWTGR-VKSFDAVGSIGDGLMPLLYKSAPEVALFSA 570
           PGI+I     S  L+ YYN+S  RD T + +  F AV +I  G        AP++  +SA
Sbjct: 523 PGIIIPSAEDSKVLLKYYNSSLVRDGTTKEIVRFGAVAAIAGGQNANFSNRAPKIMYYSA 582

Query: 571 RGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMAAPHIA 630
           RGP+ +D  F DAD+LKP+++APG+ IW AWS   T+   + GE FAM+SGTSMAAPH+A
Sbjct: 583 RGPDPQDSLFNDADILKPNLVAPGNSIWGAWSSAATESTEFEGESFAMMSGTSMAAPHVA 642

Query: 631 GIAALVKQKHPNWSPAAIKSALMTTSTTMDRGGRPLKAQQFSETEAMKLVSATPFDYGSG 690
           G+AALVKQK   +SP+AI SAL TTS   D  G  + AQ+        +  ATPFD G+G
Sbjct: 643 GVAALVKQKFRKFSPSAIASALSTTSVLFDNKGEAIMAQRAYANPDQTISPATPFDMGNG 702

Query: 691 HVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTNSPC---NFTMGHPWNLNSP 750
            VN  AALDPGLIFD  +EDY+ FLC   G +   + NYT + C   N T+    +LN P
Sbjct: 703 FVNATAALDPGLIFDTSFEDYMSFLCGING-SAPVVFNYTGTNCLRNNATISGS-DLNLP 762

Query: 751 SITIAHLVGSQTVTRTVINVAEEETYTITARMDPAVAIEVNPPAMTLRSGSSRKFSVTLT 810
           SIT++ L  ++TV R + N+A  ETYT++      V I V+P   ++ SG ++  SV LT
Sbjct: 763 SITVSKLNNTRTVQRLMTNIAGNETYTVSLITPFDVLINVSPTQFSIASGETKLLSVILT 822

Query: 811 ARSLTGTYSFGQVLLKGSRGHKVRIPV 829
           A+  +   SFG + L G+ GH VRIPV
Sbjct: 823 AKRNSSISSFGGIKLLGNAGHIVRIPV 846

BLAST of Lsi11G007880 vs. ExPASy Swiss-Prot
Match: Q9SA75 (Subtilisin-like protease SBT2.1 OS=Arabidopsis thaliana OX=3702 GN=SBT2.1 PE=2 SV=1)

HSP 1 Score: 639.0 bits (1647), Expect = 7.3e-182
Identity = 360/810 (44.44%), Postives = 499/810 (61.60%), Query Frame = 0

Query: 42  IYIVTIEGEPIVSYKGDLDGFEATAMESDEKIDPTSEIV---TSYARHLENKHDMLLGML 101
           +YIVT++  P V + G        + +S   +  TS  +    + +  +   HD LL  +
Sbjct: 36  VYIVTLKDRPSVHFSG------RESSDSKHSLTATSSQIYRTLNRSASIIRVHDSLLRNV 95

Query: 102 FERGSFKKLYSYKHLINGFAVDITHEQAEILRHTPIVKSVERDWKVRKLTTHTPEFLGLP 161
             + ++ KLYSY +LINGF+  +T +QA+ L     V++V  D+ V K TTHTP+FLGLP
Sbjct: 96  LRKENYLKLYSYHYLINGFSAVLTRKQADRLAAREEVENVVLDFLVEKATTHTPQFLGLP 155

Query: 162 TGVWPTGGGFDRAGEDIVIGFVDSGISPHHPSFA------TYNTEPFGPCLKYKGKCEID 221
            G W   GG + AGE +VIGF+D+GI P HPSF+      TY+  P      + G CE+ 
Sbjct: 156 RGAWLRDGGSEYAGEGVVIGFIDTGIDPTHPSFSDKISGHTYSVPP-----HFTGVCEVT 215

Query: 222 PDTKKDFCNGKIVGAQHFAEAAKAAGAFNPAIHFASPLDGDGHGSHTAAIAAGNNGIPVR 281
                  CN K++GA+HFAE+A + G  N +   ASP DG+GHG+HTA++AAGN+GIPV 
Sbjct: 216 IGFPPGSCNRKLIGARHFAESALSRGVLNSSQDDASPFDGEGHGTHTASVAAGNHGIPVV 275

Query: 282 MHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGVDILSLSVGPNSPP 341
           + G+  G ASGMAPRA IA+YKALY+ FGGF AD++AAIDQA  DGVDI++LS+ PN  P
Sbjct: 276 VAGHRLGNASGMAPRAHIAIYKALYKRFGGFAADIIAAIDQAAQDGVDIINLSITPNRRP 335

Query: 342 ATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIATVAAAIDDRRYKN 401
                T+ NP D  LLSAVKAG+FV QAAGN GP PK++ S+SPWI TV A   DR Y N
Sbjct: 336 PGI-ATFFNPIDMALLSAVKAGIFVVQAAGNTGPAPKSMSSFSPWIFTVGATSHDRVYSN 395

Query: 402 HLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVTKYS--PSDCQKPEVLNKRLV 461
            + LGN   + G+GL+  T +   + LV A   L + +    +    +CQ     +++LV
Sbjct: 396 SIILGNNVTIPGVGLASGTRI--MHKLVLATHALRNGTTVMDAIYVGECQDSSSFDQKLV 455

Query: 462 EGKVLLCGYSFSFVVGTASIKKVSQTAKSLGAAGFVLAVENISPGAKFDPVPVGIPGILI 521
           +GK+L+C Y+  F++G ++IK+   TAK+L AAG V  ++  + G +    P+ IPGILI
Sbjct: 456 QGKILVCSYTVRFILGVSTIKQALLTAKNLTAAGLVFYIDPSATGFQMTSSPMDIPGILI 515

Query: 522 TDVSKSMDLIDYYNTSTPRD-WTGRVKSFDAVGSIGDGLMPLLYKSAPEVALFSARGPNI 581
           +    S  L+ YYN+S  R+  +G++    +V  I  G+ P    +AP+V  FSARGP+ 
Sbjct: 516 SSPQDSQALLRYYNSSLLRENGSGKIVGSASVAKIVGGMRPTYGITAPKVMYFSARGPDP 575

Query: 582 RDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMAAPHIAGIAAL 641
            D SF DAD++KP+++APG+ IW AWSP G    ++ GE FAM SGTSM+APH+ GIAAL
Sbjct: 576 EDDSFVDADIMKPNLVAPGNAIWGAWSPLGIGTNDFQGERFAMESGTSMSAPHVTGIAAL 635

Query: 642 VKQKHPNWSPAAIKSALMTTSTTMDRGGRPLKAQQFSETEAMKLVSATPFDYGSGHVNPR 701
           +KQK P+++PAAI SAL TT++  DR G  + AQ+      +    ATPFD GSG VN  
Sbjct: 636 IKQKFPHFTPAAIASALSTTASLSDRKGEHIMAQRTVLNPDISQSPATPFDMGSGFVNAT 695

Query: 702 AALDPGLIFDAGYEDYLGFLCTTAGINVHE--IHNYTNSPC---NFTMGHPWNLNSPSIT 761
           AALDPGLIFD GY +Y+ FLC   GIN     + NYT   C   N ++    +LN PS+T
Sbjct: 696 AALDPGLIFDIGYNEYMKFLC---GINGSSPVVLNYTGESCSSYNSSLAAS-DLNLPSVT 755

Query: 762 IAHLVGSQTVTRTVINV---AEEETYTITARMDPAVAIEVNPPAMTLRSGSSRKFSVTLT 821
           IA LVG++ V R V N+   A  ETY +      +V+++V+P   T+ +G +R  S+   
Sbjct: 756 IAKLVGTRAVLRWVTNIATTATNETYIVGWMAPDSVSVKVSPAKFTIGNGQTRVLSLVFR 815

Query: 822 ARSLTGTYSFGQVLLKGSRGHKVRIPVVAM 832
           A       SFG++ L G RGH V IPV  +
Sbjct: 816 AMKNVSMASFGRIGLFGDRGHVVNIPVAVI 827

BLAST of Lsi11G007880 vs. ExPASy TrEMBL
Match: A0A5D3CEZ7 (Subtilisin-like protease SBT2.5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1411G00120 PE=3 SV=1)

HSP 1 Score: 1602.8 bits (4149), Expect = 0.0e+00
Identity = 796/816 (97.55%), Postives = 807/816 (98.90%), Query Frame = 0

Query: 20  MVVNFQHTFLVFLAILVVGKAEIYIVTIEGEPIVSYKGDLDGFEATAMESDEKIDPTSEI 79
           MVVNFQ+T LVFLAIL VGKAEIYIVTI+GEPIVSYKGDLDGFEATAMESDEKIDPTSEI
Sbjct: 1   MVVNFQYTVLVFLAILFVGKAEIYIVTIDGEPIVSYKGDLDGFEATAMESDEKIDPTSEI 60

Query: 80  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITHEQAEILRHTPIVKSVE 139
           VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITHEQAE LR TPIVKSVE
Sbjct: 61  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITHEQAETLRRTPIVKSVE 120

Query: 140 RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGISPHHPSFATYNTEPF 199
           RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGI PHHPSFATYNTEPF
Sbjct: 121 RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180

Query: 200 GPCLKYKGKCEIDPDTKKDFCNGKIVGAQHFAEAAKAAGAFNPAIHFASPLDGDGHGSHT 259
           GPC+KYKGKCE+DP+TKKDFCNGKIVGA+HFAEAAKAAGAFNP IHFASPLDGDGHGSHT
Sbjct: 181 GPCMKYKGKCEVDPNTKKDFCNGKIVGARHFAEAAKAAGAFNPDIHFASPLDGDGHGSHT 240

Query: 260 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 319
           AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV
Sbjct: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300

Query: 320 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 379
           DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA
Sbjct: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360

Query: 380 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVTKYSPSDC 439
           TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVTKYSPSDC
Sbjct: 361 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVTKYSPSDC 420

Query: 440 QKPEVLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTAKSLGAAGFVLAVENISPGAKFD 499
           QKPE+LNK LVEGKVLLCGYSFSFVVGTASIKKVSQTAK+LGAAGFVLAVENISPGAKFD
Sbjct: 421 QKPELLNKHLVEGKVLLCGYSFSFVVGTASIKKVSQTAKALGAAGFVLAVENISPGAKFD 480

Query: 500 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 559
           PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV
Sbjct: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 540

Query: 560 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 619
           ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA
Sbjct: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600

Query: 620 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRGGRPLKAQQFSETEAMKLVSATPF 679
           APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRGGRPLKAQQFSETEAMKLV+ATPF
Sbjct: 601 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRGGRPLKAQQFSETEAMKLVTATPF 660

Query: 680 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTNSPCNFTMGHPWNLN 739
           DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTNSPCNFTMGHPWNLN
Sbjct: 661 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTNSPCNFTMGHPWNLN 720

Query: 740 SPSITIAHLVGSQTVTRTVINVAEEETYTITARMDPAVAIEVNPPAMTLRSGSSRKFSVT 799
           SPSITIAHLVG++ VTRTV NVAEEETYTITARMDPAVAIEVNPPAMTLRSGSSRKFSVT
Sbjct: 721 SPSITIAHLVGTKIVTRTVTNVAEEETYTITARMDPAVAIEVNPPAMTLRSGSSRKFSVT 780

Query: 800 LTARSLTGTYSFGQVLLKGSRGHKVRIPVVAMGYQR 836
           LTARSLTGTYSFGQVLLKGSRGHKVRIPVVAMGYQR
Sbjct: 781 LTARSLTGTYSFGQVLLKGSRGHKVRIPVVAMGYQR 816

BLAST of Lsi11G007880 vs. ExPASy TrEMBL
Match: A0A1S3BRZ6 (subtilisin-like protease SBT2.5 OS=Cucumis melo OX=3656 GN=LOC103492606 PE=3 SV=1)

HSP 1 Score: 1602.4 bits (4148), Expect = 0.0e+00
Identity = 796/816 (97.55%), Postives = 807/816 (98.90%), Query Frame = 0

Query: 20  MVVNFQHTFLVFLAILVVGKAEIYIVTIEGEPIVSYKGDLDGFEATAMESDEKIDPTSEI 79
           MVVNFQ+T LVFLAIL VGKAEIYIVTI+GEPIVSYKGDLDGFEATAMESDEKIDPTSEI
Sbjct: 1   MVVNFQYTVLVFLAILFVGKAEIYIVTIDGEPIVSYKGDLDGFEATAMESDEKIDPTSEI 60

Query: 80  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITHEQAEILRHTPIVKSVE 139
           VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITHEQAE LR TPIVKSVE
Sbjct: 61  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITHEQAESLRRTPIVKSVE 120

Query: 140 RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGISPHHPSFATYNTEPF 199
           RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGI PHHPSFATYNTEPF
Sbjct: 121 RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180

Query: 200 GPCLKYKGKCEIDPDTKKDFCNGKIVGAQHFAEAAKAAGAFNPAIHFASPLDGDGHGSHT 259
           GPC+KYKGKCE+DP+TKKDFCNGKIVGA+HFAEAAKAAGAFNP IHFASPLDGDGHGSHT
Sbjct: 181 GPCMKYKGKCEVDPNTKKDFCNGKIVGARHFAEAAKAAGAFNPDIHFASPLDGDGHGSHT 240

Query: 260 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 319
           AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV
Sbjct: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300

Query: 320 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 379
           DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA
Sbjct: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360

Query: 380 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVTKYSPSDC 439
           TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVTKYSPSDC
Sbjct: 361 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVTKYSPSDC 420

Query: 440 QKPEVLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTAKSLGAAGFVLAVENISPGAKFD 499
           QKPE+LNK LVEGKVLLCGYSFSFVVGTASIKKVSQTAK+LGAAGFVLAVENISPGAKFD
Sbjct: 421 QKPELLNKHLVEGKVLLCGYSFSFVVGTASIKKVSQTAKALGAAGFVLAVENISPGAKFD 480

Query: 500 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 559
           PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV
Sbjct: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 540

Query: 560 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 619
           ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA
Sbjct: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600

Query: 620 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRGGRPLKAQQFSETEAMKLVSATPF 679
           APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRGGRPLKAQQFSETEAMKLV+ATPF
Sbjct: 601 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRGGRPLKAQQFSETEAMKLVTATPF 660

Query: 680 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTNSPCNFTMGHPWNLN 739
           DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTNSPCNFTMGHPWNLN
Sbjct: 661 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTNSPCNFTMGHPWNLN 720

Query: 740 SPSITIAHLVGSQTVTRTVINVAEEETYTITARMDPAVAIEVNPPAMTLRSGSSRKFSVT 799
           SPSITIAHLVG++ VTRTV NVAEEETYTITARMDPAVAIEVNPPAMTLRSGSSRKFSVT
Sbjct: 721 SPSITIAHLVGTKIVTRTVTNVAEEETYTITARMDPAVAIEVNPPAMTLRSGSSRKFSVT 780

Query: 800 LTARSLTGTYSFGQVLLKGSRGHKVRIPVVAMGYQR 836
           LTARSLTGTYSFGQVLLKGSRGHKVRIPVVAMGYQR
Sbjct: 781 LTARSLTGTYSFGQVLLKGSRGHKVRIPVVAMGYQR 816

BLAST of Lsi11G007880 vs. ExPASy TrEMBL
Match: A0A5A7SQC1 (Subtilisin-like protease SBT2.5 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold506G00020 PE=3 SV=1)

HSP 1 Score: 1602.4 bits (4148), Expect = 0.0e+00
Identity = 796/816 (97.55%), Postives = 807/816 (98.90%), Query Frame = 0

Query: 20  MVVNFQHTFLVFLAILVVGKAEIYIVTIEGEPIVSYKGDLDGFEATAMESDEKIDPTSEI 79
           MVVNFQ+T LVFLAIL VGKAEIYIVTI+GEPIVSYKGDLDGFEATAMESDEKIDPTSEI
Sbjct: 1   MVVNFQYTVLVFLAILFVGKAEIYIVTIDGEPIVSYKGDLDGFEATAMESDEKIDPTSEI 60

Query: 80  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITHEQAEILRHTPIVKSVE 139
           VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITHEQAE LR TPIVKSVE
Sbjct: 61  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITHEQAESLRRTPIVKSVE 120

Query: 140 RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGISPHHPSFATYNTEPF 199
           RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGI PHHPSFATYNTEPF
Sbjct: 121 RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180

Query: 200 GPCLKYKGKCEIDPDTKKDFCNGKIVGAQHFAEAAKAAGAFNPAIHFASPLDGDGHGSHT 259
           GPC+KYKGKCE+DP+TKKDFCNGKIVGA+HFAEAAKAAGAFNP IHFASPLDGDGHGSHT
Sbjct: 181 GPCMKYKGKCEVDPNTKKDFCNGKIVGARHFAEAAKAAGAFNPDIHFASPLDGDGHGSHT 240

Query: 260 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 319
           AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV
Sbjct: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300

Query: 320 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 379
           DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA
Sbjct: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360

Query: 380 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVTKYSPSDC 439
           TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVTKYSPSDC
Sbjct: 361 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVTKYSPSDC 420

Query: 440 QKPEVLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTAKSLGAAGFVLAVENISPGAKFD 499
           QKPE+LNK LVEGKVLLCGYSFSFVVGTASIKKVSQTAK+LGAAGFVLAVENISPGAKFD
Sbjct: 421 QKPELLNKHLVEGKVLLCGYSFSFVVGTASIKKVSQTAKALGAAGFVLAVENISPGAKFD 480

Query: 500 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 559
           PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV
Sbjct: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 540

Query: 560 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 619
           ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA
Sbjct: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600

Query: 620 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRGGRPLKAQQFSETEAMKLVSATPF 679
           APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRGGRPLKAQQFSETEAMKLV+ATPF
Sbjct: 601 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRGGRPLKAQQFSETEAMKLVTATPF 660

Query: 680 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTNSPCNFTMGHPWNLN 739
           DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTNSPCNFTMGHPWNLN
Sbjct: 661 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTNSPCNFTMGHPWNLN 720

Query: 740 SPSITIAHLVGSQTVTRTVINVAEEETYTITARMDPAVAIEVNPPAMTLRSGSSRKFSVT 799
           SPSITIAHLVG++ VTRTV NVAEEETYTITARMDPAVAIEVNPPAMTLRSGSSRKFSVT
Sbjct: 721 SPSITIAHLVGTKIVTRTVTNVAEEETYTITARMDPAVAIEVNPPAMTLRSGSSRKFSVT 780

Query: 800 LTARSLTGTYSFGQVLLKGSRGHKVRIPVVAMGYQR 836
           LTARSLTGTYSFGQVLLKGSRGHKVRIPVVAMGYQR
Sbjct: 781 LTARSLTGTYSFGQVLLKGSRGHKVRIPVVAMGYQR 816

BLAST of Lsi11G007880 vs. ExPASy TrEMBL
Match: A0A0A0LKB8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G060530 PE=3 SV=1)

HSP 1 Score: 1600.9 bits (4144), Expect = 0.0e+00
Identity = 797/816 (97.67%), Postives = 805/816 (98.65%), Query Frame = 0

Query: 20  MVVNFQHTFLVFLAILVVGKAEIYIVTIEGEPIVSYKGDLDGFEATAMESDEKIDPTSEI 79
           MVVNFQ+T LVFLAIL VGKAEIYIVTIEGEPIVSYKGDLDGFEATAMESDEKIDPTSEI
Sbjct: 1   MVVNFQYTVLVFLAILFVGKAEIYIVTIEGEPIVSYKGDLDGFEATAMESDEKIDPTSEI 60

Query: 80  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITHEQAEILRHTPIVKSVE 139
           VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITHEQAE LR TPIVKSVE
Sbjct: 61  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITHEQAETLRRTPIVKSVE 120

Query: 140 RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGISPHHPSFATYNTEPF 199
           RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGI PHHPSFATYNTEPF
Sbjct: 121 RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180

Query: 200 GPCLKYKGKCEIDPDTKKDFCNGKIVGAQHFAEAAKAAGAFNPAIHFASPLDGDGHGSHT 259
           GPC+KYKGKCE+DP+TKKDFCNGKIVGAQHFAEAAKAAGAFNP IHFASPLDGDGHGSHT
Sbjct: 181 GPCMKYKGKCEVDPNTKKDFCNGKIVGAQHFAEAAKAAGAFNPDIHFASPLDGDGHGSHT 240

Query: 260 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 319
           AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV
Sbjct: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300

Query: 320 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 379
           DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA
Sbjct: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360

Query: 380 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVTKYSPSDC 439
           TVAAAIDDRRYKNHLTLGNGKILAGLGLSP+THLNRTYTLVAANDVLLDSSVTKYSPSDC
Sbjct: 361 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPSTHLNRTYTLVAANDVLLDSSVTKYSPSDC 420

Query: 440 QKPEVLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTAKSLGAAGFVLAVENISPGAKFD 499
           QKPEVLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTAK+LGAAGFVLAVENISPGAKFD
Sbjct: 421 QKPEVLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTAKALGAAGFVLAVENISPGAKFD 480

Query: 500 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 559
           PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV
Sbjct: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 540

Query: 560 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 619
           ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA
Sbjct: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600

Query: 620 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRGGRPLKAQQFSETEAMKLVSATPF 679
           APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRGGRPLKAQQFSETEAMKLV+ATPF
Sbjct: 601 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRGGRPLKAQQFSETEAMKLVTATPF 660

Query: 680 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTNSPCNFTMGHPWNLN 739
           DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTNS CNFTMGHPWNLN
Sbjct: 661 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTNSACNFTMGHPWNLN 720

Query: 740 SPSITIAHLVGSQTVTRTVINVAEEETYTITARMDPAVAIEVNPPAMTLRSGSSRKFSVT 799
           SPSITIAHLVG+Q VTR V NVAEEETYTITARMDPAVAIEVNPPAMTL SGSSRKFSVT
Sbjct: 721 SPSITIAHLVGTQIVTRRVTNVAEEETYTITARMDPAVAIEVNPPAMTLLSGSSRKFSVT 780

Query: 800 LTARSLTGTYSFGQVLLKGSRGHKVRIPVVAMGYQR 836
           LTARSLTGTYSFGQVLLKGSRGHKVRIPVVAMGYQR
Sbjct: 781 LTARSLTGTYSFGQVLLKGSRGHKVRIPVVAMGYQR 816

BLAST of Lsi11G007880 vs. ExPASy TrEMBL
Match: A0A6J1KAV8 (subtilisin-like protease SBT2.5 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111491718 PE=3 SV=1)

HSP 1 Score: 1587.8 bits (4110), Expect = 0.0e+00
Identity = 787/816 (96.45%), Postives = 805/816 (98.65%), Query Frame = 0

Query: 20  MVVNFQHTFLVFLAILVVGKAEIYIVTIEGEPIVSYKGDLDGFEATAMESDEKIDPTSEI 79
           MVVNFQ T LVFL +LVVGKAEIYIVTIEGEPIVSYKGDLDGFEATAMES+EKIDPTSEI
Sbjct: 1   MVVNFQCTVLVFLVLLVVGKAEIYIVTIEGEPIVSYKGDLDGFEATAMESNEKIDPTSEI 60

Query: 80  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITHEQAEILRHTPIVKSVE 139
           VTSYARHLE+KHDMLLGMLFERGSFKKLYSYKHLINGFAVDIT EQAE LRHTPIVKSVE
Sbjct: 61  VTSYARHLESKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITPEQAETLRHTPIVKSVE 120

Query: 140 RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGISPHHPSFATYNTEPF 199
            DWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGI PHHPSFATYNTEPF
Sbjct: 121 MDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180

Query: 200 GPCLKYKGKCEIDPDTKKDFCNGKIVGAQHFAEAAKAAGAFNPAIHFASPLDGDGHGSHT 259
           GPCLKYKGKCE+DPDTKK+FCNGKIVGAQHFAEAAKAAGAFNPAIHF SPLDGDGHGSHT
Sbjct: 181 GPCLKYKGKCEVDPDTKKEFCNGKIVGAQHFAEAAKAAGAFNPAIHFESPLDGDGHGSHT 240

Query: 260 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 319
           AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV
Sbjct: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300

Query: 320 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 379
           DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA
Sbjct: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360

Query: 380 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVTKYSPSDC 439
           TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSV+KYSPSDC
Sbjct: 361 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVSKYSPSDC 420

Query: 440 QKPEVLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTAKSLGAAGFVLAVENISPGAKFD 499
           Q+P+VLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTA++LGAAGFVLAVENISPG+KFD
Sbjct: 421 QRPDVLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTARALGAAGFVLAVENISPGSKFD 480

Query: 500 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 559
           PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV
Sbjct: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 540

Query: 560 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 619
           ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA
Sbjct: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600

Query: 620 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRGGRPLKAQQFSETEAMKLVSATPF 679
           APHIAGI+ALVKQKHPNWSPAAIKSALMTTSTTMDR GRPLKAQQFSETEAMKLVSATPF
Sbjct: 601 APHIAGISALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEAMKLVSATPF 660

Query: 680 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTNSPCNFTMGHPWNLN 739
           DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTNSPCNFTMGHPWNLN
Sbjct: 661 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTNSPCNFTMGHPWNLN 720

Query: 740 SPSITIAHLVGSQTVTRTVINVAEEETYTITARMDPAVAIEVNPPAMTLRSGSSRKFSVT 799
           +PSITIAHLVG++TVTRTV NVAEEETYTITARMDPAVAIE +PPAMTLRSGSSRKFSVT
Sbjct: 721 TPSITIAHLVGTKTVTRTVTNVAEEETYTITARMDPAVAIETSPPAMTLRSGSSRKFSVT 780

Query: 800 LTARSLTGTYSFGQVLLKGSRGHKVRIPVVAMGYQR 836
           LT+RSLTGTYSFG+VLLKGSRGHKVRIPVVAMGYQR
Sbjct: 781 LTSRSLTGTYSFGEVLLKGSRGHKVRIPVVAMGYQR 816

BLAST of Lsi11G007880 vs. NCBI nr
Match: XP_038890136.1 (subtilisin-like protease SBT2.5 isoform X1 [Benincasa hispida] >XP_038890137.1 subtilisin-like protease SBT2.5 isoform X1 [Benincasa hispida] >XP_038890138.1 subtilisin-like protease SBT2.5 isoform X1 [Benincasa hispida])

HSP 1 Score: 1619.4 bits (4192), Expect = 0.0e+00
Identity = 807/816 (98.90%), Postives = 811/816 (99.39%), Query Frame = 0

Query: 20  MVVNFQHTFLVFLAILVVGKAEIYIVTIEGEPIVSYKGDLDGFEATAMESDEKIDPTSEI 79
           MVVNFQ + LVFLAILVVGKAEIYIVTIEGEPIVSYKGDLDGFEATAMESDEKIDPTSEI
Sbjct: 1   MVVNFQCSVLVFLAILVVGKAEIYIVTIEGEPIVSYKGDLDGFEATAMESDEKIDPTSEI 60

Query: 80  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITHEQAEILRHTPIVKSVE 139
           VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITHEQAEILRHTPIVKSVE
Sbjct: 61  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITHEQAEILRHTPIVKSVE 120

Query: 140 RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGISPHHPSFATYNTEPF 199
           RDWKVR+LTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGISPHHPSFATYNTEPF
Sbjct: 121 RDWKVRRLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGISPHHPSFATYNTEPF 180

Query: 200 GPCLKYKGKCEIDPDTKKDFCNGKIVGAQHFAEAAKAAGAFNPAIHFASPLDGDGHGSHT 259
           GPCLKYKGKCE+DPDTKKDFCNGKIVGAQHFAEAAKAAGAFNPAIHFASPLDGDGHGSHT
Sbjct: 181 GPCLKYKGKCEVDPDTKKDFCNGKIVGAQHFAEAAKAAGAFNPAIHFASPLDGDGHGSHT 240

Query: 260 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 319
           AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV
Sbjct: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300

Query: 320 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 379
           DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA
Sbjct: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360

Query: 380 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVTKYSPSDC 439
           TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSV KYSPSDC
Sbjct: 361 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVMKYSPSDC 420

Query: 440 QKPEVLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTAKSLGAAGFVLAVENISPGAKFD 499
           QKPEVLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTAK+LGAAGFVLAVENISPGAKFD
Sbjct: 421 QKPEVLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTAKALGAAGFVLAVENISPGAKFD 480

Query: 500 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 559
           PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV
Sbjct: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 540

Query: 560 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 619
           ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA
Sbjct: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600

Query: 620 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRGGRPLKAQQFSETEAMKLVSATPF 679
           APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRGGRPLKAQQFSETEAMKLVSATPF
Sbjct: 601 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRGGRPLKAQQFSETEAMKLVSATPF 660

Query: 680 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTNSPCNFTMGHPWNLN 739
           DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTNSPCNFTMGHPWNLN
Sbjct: 661 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTNSPCNFTMGHPWNLN 720

Query: 740 SPSITIAHLVGSQTVTRTVINVAEEETYTITARMDPAVAIEVNPPAMTLRSGSSRKFSVT 799
           SPSITIAHLVGSQTVTRTV NVAEEETYTITARMDPAVAIEVNPPAMTL SGSSRKFSVT
Sbjct: 721 SPSITIAHLVGSQTVTRTVTNVAEEETYTITARMDPAVAIEVNPPAMTLLSGSSRKFSVT 780

Query: 800 LTARSLTGTYSFGQVLLKGSRGHKVRIPVVAMGYQR 836
           LTARSLTGTYSFGQVLLKGSRGHKVRIPVVAMGYQR
Sbjct: 781 LTARSLTGTYSFGQVLLKGSRGHKVRIPVVAMGYQR 816

BLAST of Lsi11G007880 vs. NCBI nr
Match: TYK08889.1 (subtilisin-like protease SBT2.5 [Cucumis melo var. makuwa])

HSP 1 Score: 1602.8 bits (4149), Expect = 0.0e+00
Identity = 796/816 (97.55%), Postives = 807/816 (98.90%), Query Frame = 0

Query: 20  MVVNFQHTFLVFLAILVVGKAEIYIVTIEGEPIVSYKGDLDGFEATAMESDEKIDPTSEI 79
           MVVNFQ+T LVFLAIL VGKAEIYIVTI+GEPIVSYKGDLDGFEATAMESDEKIDPTSEI
Sbjct: 1   MVVNFQYTVLVFLAILFVGKAEIYIVTIDGEPIVSYKGDLDGFEATAMESDEKIDPTSEI 60

Query: 80  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITHEQAEILRHTPIVKSVE 139
           VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITHEQAE LR TPIVKSVE
Sbjct: 61  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITHEQAETLRRTPIVKSVE 120

Query: 140 RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGISPHHPSFATYNTEPF 199
           RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGI PHHPSFATYNTEPF
Sbjct: 121 RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180

Query: 200 GPCLKYKGKCEIDPDTKKDFCNGKIVGAQHFAEAAKAAGAFNPAIHFASPLDGDGHGSHT 259
           GPC+KYKGKCE+DP+TKKDFCNGKIVGA+HFAEAAKAAGAFNP IHFASPLDGDGHGSHT
Sbjct: 181 GPCMKYKGKCEVDPNTKKDFCNGKIVGARHFAEAAKAAGAFNPDIHFASPLDGDGHGSHT 240

Query: 260 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 319
           AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV
Sbjct: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300

Query: 320 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 379
           DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA
Sbjct: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360

Query: 380 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVTKYSPSDC 439
           TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVTKYSPSDC
Sbjct: 361 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVTKYSPSDC 420

Query: 440 QKPEVLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTAKSLGAAGFVLAVENISPGAKFD 499
           QKPE+LNK LVEGKVLLCGYSFSFVVGTASIKKVSQTAK+LGAAGFVLAVENISPGAKFD
Sbjct: 421 QKPELLNKHLVEGKVLLCGYSFSFVVGTASIKKVSQTAKALGAAGFVLAVENISPGAKFD 480

Query: 500 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 559
           PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV
Sbjct: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 540

Query: 560 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 619
           ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA
Sbjct: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600

Query: 620 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRGGRPLKAQQFSETEAMKLVSATPF 679
           APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRGGRPLKAQQFSETEAMKLV+ATPF
Sbjct: 601 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRGGRPLKAQQFSETEAMKLVTATPF 660

Query: 680 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTNSPCNFTMGHPWNLN 739
           DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTNSPCNFTMGHPWNLN
Sbjct: 661 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTNSPCNFTMGHPWNLN 720

Query: 740 SPSITIAHLVGSQTVTRTVINVAEEETYTITARMDPAVAIEVNPPAMTLRSGSSRKFSVT 799
           SPSITIAHLVG++ VTRTV NVAEEETYTITARMDPAVAIEVNPPAMTLRSGSSRKFSVT
Sbjct: 721 SPSITIAHLVGTKIVTRTVTNVAEEETYTITARMDPAVAIEVNPPAMTLRSGSSRKFSVT 780

Query: 800 LTARSLTGTYSFGQVLLKGSRGHKVRIPVVAMGYQR 836
           LTARSLTGTYSFGQVLLKGSRGHKVRIPVVAMGYQR
Sbjct: 781 LTARSLTGTYSFGQVLLKGSRGHKVRIPVVAMGYQR 816

BLAST of Lsi11G007880 vs. NCBI nr
Match: XP_008451260.1 (PREDICTED: subtilisin-like protease SBT2.5 [Cucumis melo] >XP_008451267.1 PREDICTED: subtilisin-like protease SBT2.5 [Cucumis melo] >XP_008451274.1 PREDICTED: subtilisin-like protease SBT2.5 [Cucumis melo] >XP_008451284.1 PREDICTED: subtilisin-like protease SBT2.5 [Cucumis melo] >XP_008451292.1 PREDICTED: subtilisin-like protease SBT2.5 [Cucumis melo] >XP_008451297.1 PREDICTED: subtilisin-like protease SBT2.5 [Cucumis melo] >XP_008451306.1 PREDICTED: subtilisin-like protease SBT2.5 [Cucumis melo] >XP_008451316.1 PREDICTED: subtilisin-like protease SBT2.5 [Cucumis melo] >KAA0031741.1 subtilisin-like protease SBT2.5 [Cucumis melo var. makuwa])

HSP 1 Score: 1602.4 bits (4148), Expect = 0.0e+00
Identity = 796/816 (97.55%), Postives = 807/816 (98.90%), Query Frame = 0

Query: 20  MVVNFQHTFLVFLAILVVGKAEIYIVTIEGEPIVSYKGDLDGFEATAMESDEKIDPTSEI 79
           MVVNFQ+T LVFLAIL VGKAEIYIVTI+GEPIVSYKGDLDGFEATAMESDEKIDPTSEI
Sbjct: 1   MVVNFQYTVLVFLAILFVGKAEIYIVTIDGEPIVSYKGDLDGFEATAMESDEKIDPTSEI 60

Query: 80  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITHEQAEILRHTPIVKSVE 139
           VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITHEQAE LR TPIVKSVE
Sbjct: 61  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITHEQAESLRRTPIVKSVE 120

Query: 140 RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGISPHHPSFATYNTEPF 199
           RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGI PHHPSFATYNTEPF
Sbjct: 121 RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180

Query: 200 GPCLKYKGKCEIDPDTKKDFCNGKIVGAQHFAEAAKAAGAFNPAIHFASPLDGDGHGSHT 259
           GPC+KYKGKCE+DP+TKKDFCNGKIVGA+HFAEAAKAAGAFNP IHFASPLDGDGHGSHT
Sbjct: 181 GPCMKYKGKCEVDPNTKKDFCNGKIVGARHFAEAAKAAGAFNPDIHFASPLDGDGHGSHT 240

Query: 260 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 319
           AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV
Sbjct: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300

Query: 320 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 379
           DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA
Sbjct: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360

Query: 380 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVTKYSPSDC 439
           TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVTKYSPSDC
Sbjct: 361 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVTKYSPSDC 420

Query: 440 QKPEVLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTAKSLGAAGFVLAVENISPGAKFD 499
           QKPE+LNK LVEGKVLLCGYSFSFVVGTASIKKVSQTAK+LGAAGFVLAVENISPGAKFD
Sbjct: 421 QKPELLNKHLVEGKVLLCGYSFSFVVGTASIKKVSQTAKALGAAGFVLAVENISPGAKFD 480

Query: 500 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 559
           PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV
Sbjct: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 540

Query: 560 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 619
           ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA
Sbjct: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600

Query: 620 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRGGRPLKAQQFSETEAMKLVSATPF 679
           APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRGGRPLKAQQFSETEAMKLV+ATPF
Sbjct: 601 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRGGRPLKAQQFSETEAMKLVTATPF 660

Query: 680 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTNSPCNFTMGHPWNLN 739
           DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTNSPCNFTMGHPWNLN
Sbjct: 661 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTNSPCNFTMGHPWNLN 720

Query: 740 SPSITIAHLVGSQTVTRTVINVAEEETYTITARMDPAVAIEVNPPAMTLRSGSSRKFSVT 799
           SPSITIAHLVG++ VTRTV NVAEEETYTITARMDPAVAIEVNPPAMTLRSGSSRKFSVT
Sbjct: 721 SPSITIAHLVGTKIVTRTVTNVAEEETYTITARMDPAVAIEVNPPAMTLRSGSSRKFSVT 780

Query: 800 LTARSLTGTYSFGQVLLKGSRGHKVRIPVVAMGYQR 836
           LTARSLTGTYSFGQVLLKGSRGHKVRIPVVAMGYQR
Sbjct: 781 LTARSLTGTYSFGQVLLKGSRGHKVRIPVVAMGYQR 816

BLAST of Lsi11G007880 vs. NCBI nr
Match: XP_011648950.1 (subtilisin-like protease SBT2.5 [Cucumis sativus] >XP_011648952.1 subtilisin-like protease SBT2.5 [Cucumis sativus] >XP_031736680.1 subtilisin-like protease SBT2.5 [Cucumis sativus] >KGN61172.1 hypothetical protein Csa_021327 [Cucumis sativus])

HSP 1 Score: 1600.9 bits (4144), Expect = 0.0e+00
Identity = 797/816 (97.67%), Postives = 805/816 (98.65%), Query Frame = 0

Query: 20  MVVNFQHTFLVFLAILVVGKAEIYIVTIEGEPIVSYKGDLDGFEATAMESDEKIDPTSEI 79
           MVVNFQ+T LVFLAIL VGKAEIYIVTIEGEPIVSYKGDLDGFEATAMESDEKIDPTSEI
Sbjct: 1   MVVNFQYTVLVFLAILFVGKAEIYIVTIEGEPIVSYKGDLDGFEATAMESDEKIDPTSEI 60

Query: 80  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITHEQAEILRHTPIVKSVE 139
           VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITHEQAE LR TPIVKSVE
Sbjct: 61  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITHEQAETLRRTPIVKSVE 120

Query: 140 RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGISPHHPSFATYNTEPF 199
           RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGI PHHPSFATYNTEPF
Sbjct: 121 RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180

Query: 200 GPCLKYKGKCEIDPDTKKDFCNGKIVGAQHFAEAAKAAGAFNPAIHFASPLDGDGHGSHT 259
           GPC+KYKGKCE+DP+TKKDFCNGKIVGAQHFAEAAKAAGAFNP IHFASPLDGDGHGSHT
Sbjct: 181 GPCMKYKGKCEVDPNTKKDFCNGKIVGAQHFAEAAKAAGAFNPDIHFASPLDGDGHGSHT 240

Query: 260 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 319
           AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV
Sbjct: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300

Query: 320 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 379
           DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA
Sbjct: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360

Query: 380 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVTKYSPSDC 439
           TVAAAIDDRRYKNHLTLGNGKILAGLGLSP+THLNRTYTLVAANDVLLDSSVTKYSPSDC
Sbjct: 361 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPSTHLNRTYTLVAANDVLLDSSVTKYSPSDC 420

Query: 440 QKPEVLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTAKSLGAAGFVLAVENISPGAKFD 499
           QKPEVLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTAK+LGAAGFVLAVENISPGAKFD
Sbjct: 421 QKPEVLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTAKALGAAGFVLAVENISPGAKFD 480

Query: 500 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 559
           PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV
Sbjct: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 540

Query: 560 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 619
           ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA
Sbjct: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600

Query: 620 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRGGRPLKAQQFSETEAMKLVSATPF 679
           APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRGGRPLKAQQFSETEAMKLV+ATPF
Sbjct: 601 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRGGRPLKAQQFSETEAMKLVTATPF 660

Query: 680 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTNSPCNFTMGHPWNLN 739
           DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTNS CNFTMGHPWNLN
Sbjct: 661 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTNSACNFTMGHPWNLN 720

Query: 740 SPSITIAHLVGSQTVTRTVINVAEEETYTITARMDPAVAIEVNPPAMTLRSGSSRKFSVT 799
           SPSITIAHLVG+Q VTR V NVAEEETYTITARMDPAVAIEVNPPAMTL SGSSRKFSVT
Sbjct: 721 SPSITIAHLVGTQIVTRRVTNVAEEETYTITARMDPAVAIEVNPPAMTLLSGSSRKFSVT 780

Query: 800 LTARSLTGTYSFGQVLLKGSRGHKVRIPVVAMGYQR 836
           LTARSLTGTYSFGQVLLKGSRGHKVRIPVVAMGYQR
Sbjct: 781 LTARSLTGTYSFGQVLLKGSRGHKVRIPVVAMGYQR 816

BLAST of Lsi11G007880 vs. NCBI nr
Match: XP_023546345.1 (subtilisin-like protease SBT2.5 [Cucurbita pepo subsp. pepo] >XP_023546347.1 subtilisin-like protease SBT2.5 [Cucurbita pepo subsp. pepo] >XP_023546348.1 subtilisin-like protease SBT2.5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1588.5 bits (4112), Expect = 0.0e+00
Identity = 787/816 (96.45%), Postives = 806/816 (98.77%), Query Frame = 0

Query: 20  MVVNFQHTFLVFLAILVVGKAEIYIVTIEGEPIVSYKGDLDGFEATAMESDEKIDPTSEI 79
           MVVNFQ T LVFL ++VVGKAEIYIVTIEGEPIVSYKGDLDGFEATAMES+EKIDPTSEI
Sbjct: 1   MVVNFQCTVLVFLVLVVVGKAEIYIVTIEGEPIVSYKGDLDGFEATAMESNEKIDPTSEI 60

Query: 80  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITHEQAEILRHTPIVKSVE 139
           VTSYARHLE+KHDMLLGMLFERGSFKKLYSYKHLINGFAVDIT EQAE LRHTPIVKSVE
Sbjct: 61  VTSYARHLESKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITPEQAETLRHTPIVKSVE 120

Query: 140 RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGISPHHPSFATYNTEPF 199
           RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGI PHHPSFATYNTEPF
Sbjct: 121 RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180

Query: 200 GPCLKYKGKCEIDPDTKKDFCNGKIVGAQHFAEAAKAAGAFNPAIHFASPLDGDGHGSHT 259
           GPCLKYKGKCE+DPDTKK+FCNGKIVGAQHFAEAAKAAGAFNPAIHF SPLDGDGHGSHT
Sbjct: 181 GPCLKYKGKCEVDPDTKKEFCNGKIVGAQHFAEAAKAAGAFNPAIHFESPLDGDGHGSHT 240

Query: 260 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 319
           AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV
Sbjct: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300

Query: 320 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 379
           DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA
Sbjct: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360

Query: 380 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVTKYSPSDC 439
           TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSV+KYSPSDC
Sbjct: 361 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVSKYSPSDC 420

Query: 440 QKPEVLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTAKSLGAAGFVLAVENISPGAKFD 499
           Q+P+VLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTA++LGAAGFVLAVENISPG+KFD
Sbjct: 421 QRPDVLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTARALGAAGFVLAVENISPGSKFD 480

Query: 500 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 559
           PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV
Sbjct: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 540

Query: 560 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 619
           ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA
Sbjct: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600

Query: 620 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRGGRPLKAQQFSETEAMKLVSATPF 679
           APHIAGI+ALVKQKHPNWSPAAIKSALMTTSTTMDR GRPLKAQQFSETEAMKLVSATPF
Sbjct: 601 APHIAGISALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEAMKLVSATPF 660

Query: 680 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTNSPCNFTMGHPWNLN 739
           DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHN+TNSPCNFTMGHPWNLN
Sbjct: 661 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNFTNSPCNFTMGHPWNLN 720

Query: 740 SPSITIAHLVGSQTVTRTVINVAEEETYTITARMDPAVAIEVNPPAMTLRSGSSRKFSVT 799
           +PSITIAHLVG++TVTRTV NVAEEETYTITARMDPAVAIE +PPAMTLRSGSSRKFSVT
Sbjct: 721 TPSITIAHLVGTKTVTRTVTNVAEEETYTITARMDPAVAIETSPPAMTLRSGSSRKFSVT 780

Query: 800 LTARSLTGTYSFGQVLLKGSRGHKVRIPVVAMGYQR 836
           LTARSLTGTYSFG+VLLKGSRGHKVRIPVVAMGYQR
Sbjct: 781 LTARSLTGTYSFGEVLLKGSRGHKVRIPVVAMGYQR 816

BLAST of Lsi11G007880 vs. TAIR 10
Match: AT2G19170.1 (subtilisin-like serine protease 3 )

HSP 1 Score: 1345.9 bits (3482), Expect = 0.0e+00
Identity = 662/809 (81.83%), Postives = 729/809 (90.11%), Query Frame = 0

Query: 28  FLVFLAILVVGKAEIYIVTIEGEPIVSYKGDLDGFEATAMESDEKIDPTSEIVTSYARHL 87
           F+VF+ +LV   AE+YIVT+EG+PI+SYKG  +GFEATA+ESDEKID +SE+VT YARHL
Sbjct: 8   FVVFV-LLVAVTAEVYIVTMEGDPIISYKGGENGFEATAVESDEKIDTSSELVTVYARHL 67

Query: 88  ENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITHEQAEILRHTPIVKSVERDWKVRKL 147
           E KHDM+LGMLFE GS+KKLYSYKHLINGFA  ++ EQAE LR  P V+SV++DWKVR+L
Sbjct: 68  ERKHDMILGMLFEEGSYKKLYSYKHLINGFAAHVSPEQAETLRRAPGVRSVDKDWKVRRL 127

Query: 148 TTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGISPHHPSFATYNTEPFGPCLKYKG 207
           TTHTPEFLGLPT VWPTGGGFDRAGEDIVIGFVDSGI PHHPSFA+++  P+GP   YKG
Sbjct: 128 TTHTPEFLGLPTDVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFASHHRLPYGPLPHYKG 187

Query: 208 KCEIDPDTKKDFCNGKIVGAQHFAEAAKAAGAFNPAIHFASPLDGDGHGSHTAAIAAGNN 267
           KCE DP TKK FCN KIVGAQHFAEAAKAAGAFNP I +ASP+DGDGHGSHTAAIAAGNN
Sbjct: 188 KCEEDPHTKKSFCNRKIVGAQHFAEAAKAAGAFNPDIDYASPMDGDGHGSHTAAIAAGNN 247

Query: 268 GIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGVDILSLSVG 327
           GIP+RMHGYEFGKASGMAPRARIAVYKALYR+FGGFVADVVAAIDQAVHDGVDILSLSVG
Sbjct: 248 GIPLRMHGYEFGKASGMAPRARIAVYKALYRLFGGFVADVVAAIDQAVHDGVDILSLSVG 307

Query: 328 PNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIATVAAAIDD 387
           PNSPP TTK T+LNPFDATLL AVKAGVFVAQAAGNGGPFPKTLVSYSPWI TVAAAIDD
Sbjct: 308 PNSPPTTTKTTFLNPFDATLLGAVKAGVFVAQAAGNGGPFPKTLVSYSPWITTVAAAIDD 367

Query: 388 RRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVTKYSPSDCQKPEVLNK 447
           RRYKNHLTLGNGK+LAG+GLSP T  +R YTLV+ANDVLLDSSV+KY+PSDCQ+PEV NK
Sbjct: 368 RRYKNHLTLGNGKMLAGMGLSPPTRPHRLYTLVSANDVLLDSSVSKYNPSDCQRPEVFNK 427

Query: 448 RLVEGKVLLCGYSFSFVVGTASIKKVSQTAKSLGAAGFVLAVENISPGAKFDPVPVGIPG 507
           +LVEG +LLCGYSF+FVVGTASIKKV  TAK LGAAGFVL VEN+SPG KFDPVP  IPG
Sbjct: 428 KLVEGNILLCGYSFNFVVGTASIKKVVATAKHLGAAGFVLVVENVSPGTKFDPVPSAIPG 487

Query: 508 ILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEVALFSARGP 567
           ILITDVSKSMDLIDYYN ST RDWTGRVKSF A GSIGDGL P+L+KSAP+VALFSARGP
Sbjct: 488 ILITDVSKSMDLIDYYNASTSRDWTGRVKSFKAEGSIGDGLAPVLHKSAPQVALFSARGP 547

Query: 568 NIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMAAPHIAGIA 627
           N +DFSFQDADLLKPDILAPG LIWAAW PNGTDEPNYVGEGFA+ISGTSMAAPHIAGIA
Sbjct: 548 NTKDFSFQDADLLKPDILAPGYLIWAAWCPNGTDEPNYVGEGFALISGTSMAAPHIAGIA 607

Query: 628 ALVKQKHPNWSPAAIKSALMTTSTTMDRGGRPLKAQQFSETEAMKLVSATPFDYGSGHVN 687
           ALVKQKHP WSPAAIKSALMTTST +DR GR L+AQQ+S+TEA+ LV ATPFDYGSGHVN
Sbjct: 608 ALVKQKHPQWSPAAIKSALMTTSTVIDRAGRLLQAQQYSDTEAVTLVKATPFDYGSGHVN 667

Query: 688 PRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTNSPCNFTMGHPWNLNSPSITIAH 747
           P AALDPGLIFDAGYEDYLGFLCTT GI+ HEI NYTN+ CN+ M HP N N+PSI ++H
Sbjct: 668 PSAALDPGLIFDAGYEDYLGFLCTTPGISAHEIRNYTNTACNYDMKHPSNFNAPSIAVSH 727

Query: 748 LVGSQTVTRTVINVAE-EETYTITARMDPAVAIEVNPPAMTLRSGSSRKFSVTLTARSLT 807
           LVG+QTVTR V NVAE EETYTITARM P++AIEVNPPAMTLR G++R FSVT+T RS++
Sbjct: 728 LVGTQTVTRKVTNVAEVEETYTITARMQPSIAIEVNPPAMTLRPGATRTFSVTMTVRSVS 787

Query: 808 GTYSFGQVLLKGSRGHKVRIPVVAMGYQR 836
           G YSFG+V LKGSRGHKVRIPVVA+G++R
Sbjct: 788 GVYSFGEVKLKGSRGHKVRIPVVALGHRR 815

BLAST of Lsi11G007880 vs. TAIR 10
Match: AT4G30020.1 (PA-domain containing subtilase family protein )

HSP 1 Score: 1338.6 bits (3463), Expect = 0.0e+00
Identity = 660/809 (81.58%), Postives = 726/809 (89.74%), Query Frame = 0

Query: 29  LVFLAILVVGKAEIYIVTIEGEPIVSYKGDLDGFEATAMESDEKIDPTSEIVTSYARHLE 88
           LVF    +   AEIYIVT+EGEPI+SYKG  +GFEATA+ESDEKID TSE+VTSYARHLE
Sbjct: 8   LVFFTCFLTVTAEIYIVTMEGEPIISYKGGDNGFEATAVESDEKIDTTSELVTSYARHLE 67

Query: 89  NKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITHEQAEILRHTPIVKSVERDWKVRKLT 148
            KHDMLLGMLF  GS+KKLYSYKHLINGFA  ++ +QAE+LR  P VKSV+RDWKVRKLT
Sbjct: 68  RKHDMLLGMLFVEGSYKKLYSYKHLINGFAAHVSPDQAEMLRRAPGVKSVDRDWKVRKLT 127

Query: 149 THTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGISPHHPSFATYNTE-PFGPCLKYKG 208
           THTP+FLGLPT VWPTGGG+DRAGEDIVIGF+DSGI PHHPSFA+++T  P+GP   YKG
Sbjct: 128 THTPQFLGLPTDVWPTGGGYDRAGEDIVIGFIDSGIFPHHPSFASHHTTVPYGPHPSYKG 187

Query: 209 KCEIDPDTKKDFCNGKIVGAQHFAEAAKAAGAFNPAIHFASPLDGDGHGSHTAAIAAGNN 268
           KCE DP TK  FCNGKI+GAQHFAEAAKAAGAFNP I FASP+DGDGHGSHTAAIAAGNN
Sbjct: 188 KCEEDPHTKISFCNGKIIGAQHFAEAAKAAGAFNPDIDFASPMDGDGHGSHTAAIAAGNN 247

Query: 269 GIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGVDILSLSVG 328
           GIPVRMHGYEFGKASGMAPRARIAVYKALYR+FGGFVADVVAAIDQAVHDGVDILSLSVG
Sbjct: 248 GIPVRMHGYEFGKASGMAPRARIAVYKALYRLFGGFVADVVAAIDQAVHDGVDILSLSVG 307

Query: 329 PNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIATVAAAIDD 388
           PNSPPATTK T+LNPFDATLL AVKAGVFVAQAAGNGGPFPKTLVSYSPWI TVAAAIDD
Sbjct: 308 PNSPPATTKTTFLNPFDATLLGAVKAGVFVAQAAGNGGPFPKTLVSYSPWITTVAAAIDD 367

Query: 389 RRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVTKYSPSDCQKPEVLNK 448
           RRYKNHLTLGNGK+LAG+GLSP+T  +R+Y +V+ANDVLL SS  KY+PSDCQKPEVLNK
Sbjct: 368 RRYKNHLTLGNGKMLAGIGLSPSTRPHRSYKMVSANDVLLGSSGMKYNPSDCQKPEVLNK 427

Query: 449 RLVEGKVLLCGYSFSFVVGTASIKKVSQTAKSLGAAGFVLAVENISPGAKFDPVPVGIPG 508
           +LVEG +LLCGYSF+FV G+ASIKKV++TAK LGAAGFVL VEN+SPG KFDPVP  IPG
Sbjct: 428 KLVEGNILLCGYSFNFVAGSASIKKVAETAKHLGAAGFVLVVENVSPGTKFDPVPSCIPG 487

Query: 509 ILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEVALFSARGP 568
           ILITDVSKSMDLIDYYN +T RDW GRVK F A GSIGDGL P+L+KSAPEVALFSARGP
Sbjct: 488 ILITDVSKSMDLIDYYNVTTSRDWMGRVKDFKAEGSIGDGLEPILHKSAPEVALFSARGP 547

Query: 569 NIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMAAPHIAGIA 628
           N +DFSFQDADLLKPDILAPGSLIW+AWS NGTDE NY+GEGFA+ISGTSMAAPHIAGIA
Sbjct: 548 NTKDFSFQDADLLKPDILAPGSLIWSAWSANGTDEANYIGEGFALISGTSMAAPHIAGIA 607

Query: 629 ALVKQKHPNWSPAAIKSALMTTSTTMDRGGRPLKAQQFSETEAMKLVSATPFDYGSGHVN 688
           ALVKQKHP WSPAAIKSALMTTST +DR GRPL+AQQ+SETE + LV ATPFDYGSGHVN
Sbjct: 608 ALVKQKHPQWSPAAIKSALMTTSTVIDRAGRPLQAQQYSETETVTLVKATPFDYGSGHVN 667

Query: 689 PRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTNSPCNFTMGHPWNLNSPSITIAH 748
           P AALDPGLIFDAGYEDY+GFLCTT GI+ HEI N+TN+PCNF M HP N N+PSI I+H
Sbjct: 668 PSAALDPGLIFDAGYEDYIGFLCTTPGIDAHEIKNFTNTPCNFKMVHPSNFNTPSIAISH 727

Query: 749 LVGSQTVTRTVINVA-EEETYTITARMDPAVAIEVNPPAMTLRSGSSRKFSVTLTARSLT 808
           LV +QTVTR V NVA EEETYTIT+RM+PA+AIEV+PPAMT+R+G+SR FSVTLT RS+T
Sbjct: 728 LVRTQTVTRRVTNVAEEEETYTITSRMEPAIAIEVSPPAMTVRAGASRTFSVTLTVRSVT 787

Query: 809 GTYSFGQVLLKGSRGHKVRIPVVAMGYQR 836
           G YSFGQV LKGSRGHKV +PVVAMG +R
Sbjct: 788 GAYSFGQVTLKGSRGHKVTLPVVAMGQRR 816

BLAST of Lsi11G007880 vs. TAIR 10
Match: AT5G44530.1 (Subtilase family protein )

HSP 1 Score: 661.0 bits (1704), Expect = 1.3e-189
Identity = 377/841 (44.83%), Postives = 519/841 (61.71%), Query Frame = 0

Query: 17  LETMVVNFQHTFL----VFLAILVVGKAE-------IYIVTIEGEPIVSYKGDLDGFEAT 76
           +  M+V F    L    VFL+   +G+ +       +YIVT++  PIV    + +  +  
Sbjct: 2   VRVMLVRFGFLLLMISFVFLSNNTLGQQQDDDDDSAVYIVTLKQPPIVHLFEEQE-LKHK 61

Query: 77  AMESDEKIDPTSEIVTSYARHLENK-------HDMLLGMLFERGSFKKLYSYKHLINGFA 136
             +   K+ P +    S  RH ++K       HD  L    +   + KLYSY +LINGFA
Sbjct: 62  KSKFTPKLRPRN---NSRKRHGKSKIPSVVQSHDSFLRKTLKGEKYIKLYSYHYLINGFA 121

Query: 137 VDITHEQAEILRHTPIVKSVERDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIG 196
           + I  +QAE L     V ++  D+ VR  TT+TP+F+GLP G W   GGF+ AGE ++IG
Sbjct: 122 LFINSQQAEKLSMRKEVANIVLDYSVRTATTYTPQFMGLPQGAWVKEGGFEIAGEGVIIG 181

Query: 197 FVDSGISPHHPSFATYNTEPFGPCLK-YKGKCEIDPDTKKDFCNGKIVGAQHFAEAAKAA 256
           F+D+GI P+HPSF   +++   P  K + G CE+ PD     CN K++GA+HFA++A   
Sbjct: 182 FIDTGIDPNHPSFNDNDSKRSYPIPKHFSGVCEVTPDFPSGSCNKKLIGARHFAQSAVTR 241

Query: 257 GAFNPAIHFASPLDGDGHGSHTAAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALY 316
           G FN +  +ASP DGDGHG+HTA++AAGN+G+PV +  + FG ASG+APRA I+VYKALY
Sbjct: 242 GIFNSSEDYASPFDGDGHGTHTASVAAGNHGVPVIVSNHNFGYASGIAPRAFISVYKALY 301

Query: 317 RIFGGFVADVVAAIDQAVHDGVDILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFV 376
           + FGGF ADVVAAIDQA  DGVDILSLS+ PN  P     T+ NP D  LLSAVKAG+FV
Sbjct: 302 KSFGGFAADVVAAIDQAAQDGVDILSLSITPNRKPPGV-ATFFNPIDMALLSAVKAGIFV 361

Query: 377 AQAAGNGGPFPKTLVSYSPWIATVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTY 436
            QAAGN GP PKT+ S+SPWI TV A+  DR Y N LTLGN   + G+G +  T   + Y
Sbjct: 362 VQAAGNTGPAPKTMSSFSPWIFTVGASSHDRVYSNSLTLGNNVTIPGMGFAIPTDSGKMY 421

Query: 437 TLVAANDVLLDS-SVTK-YSPSDCQKPEVLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQ 496
            +++A   L +S SV K     +CQ  E  ++  V GK+L+C YS  FV+G ++IK+   
Sbjct: 422 KMISAFHALNNSTSVDKDMYVGECQDYENFDQDRVSGKLLICSYSARFVLGLSTIKQALD 481

Query: 497 TAKSLGAAGFVLAVENISPGAKFDPVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGR- 556
            AK+L A G +  ++    G + +P P+ +PGI+I  V  S  L+ YYN+S  RD T + 
Sbjct: 482 VAKNLSATGVIFYIDPYVLGFEINPTPMDMPGIIIPSVEDSKTLLKYYNSSIQRDVTTKE 541

Query: 557 VKSFDAVGSIGDGLMPLLYKSAPEVALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAA 616
           + SF AV +I  GL       AP+V  +SARGP+  D SF DAD+LKP+++APG+ IW A
Sbjct: 542 IVSFGAVAAIEGGLNANFSNRAPKVMYYSARGPDPEDNSFNDADVLKPNLVAPGNSIWGA 601

Query: 617 WSPNGTDEPNYVGEGFAMISGTSMAAPHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMD 676
           WS   TD   + GE FAM+SGTSMAAPH+AG+AAL+KQ +P ++P+ I SAL TT+   D
Sbjct: 602 WSSASTDSTEFEGEKFAMMSGTSMAAPHVAGVAALIKQSYPQFTPSTISSALSTTALLND 661

Query: 677 RGGRPLKAQQFSETEAMKLVSATPFDYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAG 736
             G P+ AQ+        L +ATP D GSG VN  AALDPGL+FD  +EDY+ FLC   G
Sbjct: 662 NKGSPIMAQRTYSNPDQSLYTATPSDMGSGFVNATAALDPGLVFDTSFEDYISFLC---G 721

Query: 737 INVHE--IHNYT--NSPCNFTMGHPWNLNSPSITIAHLVGSQTVTRTVINVAEEETYTIT 796
           IN  +  + NYT    P N T    ++LN PSIT++ L G+QT  R++ N+A  ETY + 
Sbjct: 722 INGSDTVVFNYTGFRCPANNTPVSGFDLNLPSITVSTLSGTQTFQRSMRNIAGNETYNVG 781

Query: 797 ARMDPAVAIEVNPPAMTLRSGSSRKFSVTLTARSLTGTYSFGQVLLKGSRGHKVRIPVVA 832
                 V+++V+P   ++  G ++  SVTLT    + + SFG++ L G+ GH V IPV  
Sbjct: 782 WSPPYGVSMKVSPTQFSIAMGENQVLSVTLTVTKNSSSSSFGRIGLFGNTGHIVNIPVTV 834

BLAST of Lsi11G007880 vs. TAIR 10
Match: AT4G20430.1 (Subtilase family protein )

HSP 1 Score: 647.9 bits (1670), Expect = 1.1e-185
Identity = 351/747 (46.99%), Postives = 471/747 (63.05%), Query Frame = 0

Query: 91  HDMLLGMLFERGSFKKLYSYKHLINGFAVDITHEQAEILRHTPIVKSVERDWKVRKLTTH 150
           HD LL    +   + KLYS+ +LINGFAV ++ +QAE L     V ++  D+ VR  TT+
Sbjct: 103 HDSLLRNALKGEKYIKLYSFHYLINGFAVFVSSQQAETLSRRREVANIVLDFSVRTATTY 162

Query: 151 TPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGISPHHPSFATYNT--EPFGPCLKYKGK 210
           TP+F+GLP G W   GG++ AGE IVIGF+D+GI P HPSF   +T    +     + G 
Sbjct: 163 TPQFMGLPKGAWVKEGGYETAGEGIVIGFIDTGIDPTHPSFNGTDTSQRQYPIPNHFSGV 222

Query: 211 CEIDPDTKKDFCNGKIVGAQHFAEAAKAAGAFNPAIHFASPLDGDGHGSHTAAIAAGNNG 270
           CE+ PD     CN K+VGA+HFA++A   G FN +  +ASP DGDGHG+HTA+IAAGN+G
Sbjct: 223 CEVTPDFPSGSCNRKLVGARHFAQSAITRGIFNSSEDYASPFDGDGHGTHTASIAAGNHG 282

Query: 271 IPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGVDILSLSVGP 330
           +   + G+ FG ASG+APRA I+VYKALY+ FGGF ADVVAAIDQA  DGVDILSLS+ P
Sbjct: 283 VSAVVSGHNFGSASGIAPRAHISVYKALYKSFGGFAADVVAAIDQAAQDGVDILSLSITP 342

Query: 331 NSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIATVAAAIDDR 390
           N  P     T+ NP D  +LSAVKAG+FV QAAGN GP PK++ S+SPWI TV AA  DR
Sbjct: 343 NRRPPGV-ATFFNPLDMAMLSAVKAGIFVVQAAGNTGPSPKSMSSFSPWIFTVGAASHDR 402

Query: 391 RYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVT---KYSPSDCQKPEVL 450
            Y N + LGN   + G+GL+  T   + YT+++A D L + S          +CQ     
Sbjct: 403 DYSNSIVLGNNVSIPGVGLALRTDEGKKYTMISALDALKNKSSVVDKDMYVGECQDYGSF 462

Query: 451 NKRLVEGKVLLCGYSFSFVVGTASIKKVSQTAKSLGAAGFVLAVENISPGAKFDPVPVGI 510
           +K ++ G +L+C YS  FV+G ++IK+    AK+L A G V  ++    G + +P P+ +
Sbjct: 463 DKDVIRGNLLICSYSIRFVLGLSTIKQALAVAKNLSAKGVVFYMDPYVLGFQINPTPMDM 522

Query: 511 PGILITDVSKSMDLIDYYNTSTPRDWTGR-VKSFDAVGSIGDGLMPLLYKSAPEVALFSA 570
           PGI+I     S  L+ YYN+S  RD T + +  F AV +I  G        AP++  +SA
Sbjct: 523 PGIIIPSAEDSKVLLKYYNSSLVRDGTTKEIVRFGAVAAIAGGQNANFSNRAPKIMYYSA 582

Query: 571 RGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMAAPHIA 630
           RGP+ +D  F DAD+LKP+++APG+ IW AWS   T+   + GE FAM+SGTSMAAPH+A
Sbjct: 583 RGPDPQDSLFNDADILKPNLVAPGNSIWGAWSSAATESTEFEGESFAMMSGTSMAAPHVA 642

Query: 631 GIAALVKQKHPNWSPAAIKSALMTTSTTMDRGGRPLKAQQFSETEAMKLVSATPFDYGSG 690
           G+AALVKQK   +SP+AI SAL TTS   D  G  + AQ+        +  ATPFD G+G
Sbjct: 643 GVAALVKQKFRKFSPSAIASALSTTSVLFDNKGEAIMAQRAYANPDQTISPATPFDMGNG 702

Query: 691 HVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTNSPC---NFTMGHPWNLNSP 750
            VN  AALDPGLIFD  +EDY+ FLC   G +   + NYT + C   N T+    +LN P
Sbjct: 703 FVNATAALDPGLIFDTSFEDYMSFLCGING-SAPVVFNYTGTNCLRNNATISGS-DLNLP 762

Query: 751 SITIAHLVGSQTVTRTVINVAEEETYTITARMDPAVAIEVNPPAMTLRSGSSRKFSVTLT 810
           SIT++ L  ++TV R + N+A  ETYT++      V I V+P   ++ SG ++  SV LT
Sbjct: 763 SITVSKLNNTRTVQRLMTNIAGNETYTVSLITPFDVLINVSPTQFSIASGETKLLSVILT 822

Query: 811 ARSLTGTYSFGQVLLKGSRGHKVRIPV 829
           A+  +   SFG + L G+ GH VRIPV
Sbjct: 823 AKRNSSISSFGGIKLLGNAGHIVRIPV 846

BLAST of Lsi11G007880 vs. TAIR 10
Match: AT1G30600.1 (Subtilase family protein )

HSP 1 Score: 639.0 bits (1647), Expect = 5.2e-183
Identity = 360/810 (44.44%), Postives = 499/810 (61.60%), Query Frame = 0

Query: 42  IYIVTIEGEPIVSYKGDLDGFEATAMESDEKIDPTSEIV---TSYARHLENKHDMLLGML 101
           +YIVT++  P V + G        + +S   +  TS  +    + +  +   HD LL  +
Sbjct: 36  VYIVTLKDRPSVHFSG------RESSDSKHSLTATSSQIYRTLNRSASIIRVHDSLLRNV 95

Query: 102 FERGSFKKLYSYKHLINGFAVDITHEQAEILRHTPIVKSVERDWKVRKLTTHTPEFLGLP 161
             + ++ KLYSY +LINGF+  +T +QA+ L     V++V  D+ V K TTHTP+FLGLP
Sbjct: 96  LRKENYLKLYSYHYLINGFSAVLTRKQADRLAAREEVENVVLDFLVEKATTHTPQFLGLP 155

Query: 162 TGVWPTGGGFDRAGEDIVIGFVDSGISPHHPSFA------TYNTEPFGPCLKYKGKCEID 221
            G W   GG + AGE +VIGF+D+GI P HPSF+      TY+  P      + G CE+ 
Sbjct: 156 RGAWLRDGGSEYAGEGVVIGFIDTGIDPTHPSFSDKISGHTYSVPP-----HFTGVCEVT 215

Query: 222 PDTKKDFCNGKIVGAQHFAEAAKAAGAFNPAIHFASPLDGDGHGSHTAAIAAGNNGIPVR 281
                  CN K++GA+HFAE+A + G  N +   ASP DG+GHG+HTA++AAGN+GIPV 
Sbjct: 216 IGFPPGSCNRKLIGARHFAESALSRGVLNSSQDDASPFDGEGHGTHTASVAAGNHGIPVV 275

Query: 282 MHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGVDILSLSVGPNSPP 341
           + G+  G ASGMAPRA IA+YKALY+ FGGF AD++AAIDQA  DGVDI++LS+ PN  P
Sbjct: 276 VAGHRLGNASGMAPRAHIAIYKALYKRFGGFAADIIAAIDQAAQDGVDIINLSITPNRRP 335

Query: 342 ATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIATVAAAIDDRRYKN 401
                T+ NP D  LLSAVKAG+FV QAAGN GP PK++ S+SPWI TV A   DR Y N
Sbjct: 336 PGI-ATFFNPIDMALLSAVKAGIFVVQAAGNTGPAPKSMSSFSPWIFTVGATSHDRVYSN 395

Query: 402 HLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVTKYS--PSDCQKPEVLNKRLV 461
            + LGN   + G+GL+  T +   + LV A   L + +    +    +CQ     +++LV
Sbjct: 396 SIILGNNVTIPGVGLASGTRI--MHKLVLATHALRNGTTVMDAIYVGECQDSSSFDQKLV 455

Query: 462 EGKVLLCGYSFSFVVGTASIKKVSQTAKSLGAAGFVLAVENISPGAKFDPVPVGIPGILI 521
           +GK+L+C Y+  F++G ++IK+   TAK+L AAG V  ++  + G +    P+ IPGILI
Sbjct: 456 QGKILVCSYTVRFILGVSTIKQALLTAKNLTAAGLVFYIDPSATGFQMTSSPMDIPGILI 515

Query: 522 TDVSKSMDLIDYYNTSTPRD-WTGRVKSFDAVGSIGDGLMPLLYKSAPEVALFSARGPNI 581
           +    S  L+ YYN+S  R+  +G++    +V  I  G+ P    +AP+V  FSARGP+ 
Sbjct: 516 SSPQDSQALLRYYNSSLLRENGSGKIVGSASVAKIVGGMRPTYGITAPKVMYFSARGPDP 575

Query: 582 RDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMAAPHIAGIAAL 641
            D SF DAD++KP+++APG+ IW AWSP G    ++ GE FAM SGTSM+APH+ GIAAL
Sbjct: 576 EDDSFVDADIMKPNLVAPGNAIWGAWSPLGIGTNDFQGERFAMESGTSMSAPHVTGIAAL 635

Query: 642 VKQKHPNWSPAAIKSALMTTSTTMDRGGRPLKAQQFSETEAMKLVSATPFDYGSGHVNPR 701
           +KQK P+++PAAI SAL TT++  DR G  + AQ+      +    ATPFD GSG VN  
Sbjct: 636 IKQKFPHFTPAAIASALSTTASLSDRKGEHIMAQRTVLNPDISQSPATPFDMGSGFVNAT 695

Query: 702 AALDPGLIFDAGYEDYLGFLCTTAGINVHE--IHNYTNSPC---NFTMGHPWNLNSPSIT 761
           AALDPGLIFD GY +Y+ FLC   GIN     + NYT   C   N ++    +LN PS+T
Sbjct: 696 AALDPGLIFDIGYNEYMKFLC---GINGSSPVVLNYTGESCSSYNSSLAAS-DLNLPSVT 755

Query: 762 IAHLVGSQTVTRTVINV---AEEETYTITARMDPAVAIEVNPPAMTLRSGSSRKFSVTLT 821
           IA LVG++ V R V N+   A  ETY +      +V+++V+P   T+ +G +R  S+   
Sbjct: 756 IAKLVGTRAVLRWVTNIATTATNETYIVGWMAPDSVSVKVSPAKFTIGNGQTRVLSLVFR 815

Query: 822 ARSLTGTYSFGQVLLKGSRGHKVRIPVVAM 832
           A       SFG++ L G RGH V IPV  +
Sbjct: 816 AMKNVSMASFGRIGLFGDRGHVVNIPVAVI 827