Lsi11G007730 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi11G007730
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	nifU-like protein 3, chloroplastic
Location	chr11: 8844721 .. 8847175 (-)
RNA-Seq Expression	Lsi11G007730
Synteny	Lsi11G007730

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAAAAAAAAAAAAGGGAAATTGTTCAATTTATCCACATTCTTCCATAATTCCATTTCTCCTTCCCAATTTCCACCACAAAATTTGCCCACTCCATTGAAGATCTTCATCTTCATCTTCTTCACCCAATCACTATGTTTCCCTTCAGCAAAACCATAACCTAAATCCCCATTTGCAGCACACTTCCACAGCGAAATGGTTTAAGTCAACACTTCCTTCCATTTATCTCTTTATGATACTTAATAATCACTTGCATTTCAATGGTGGCCGCTTTCTCAACTCGACCTCAACCTCTTAAATCAACTCCAATCGCTGCAACTCTGTATCGCTCACCGTCTGACCCAACTTCACCTTCCCATTTGTCCCTCTTCAAGGTTGCTTTCTCGATTCTGTTTGTAATCTTCACCAATTTTTTATTATGCAACTTACGGGCTGCTATTATTTGAATCAGAGTTCGTTCTTTAGAGGTCAGTTTTGTAGCCAGCGCTTTCTTGGATTCAATTCAAGTTGTACTCGGAGACGCCATATAGGTAGCATTTGTGGATATCCCTTTTACGAAATTTGGCTTATTCTTGATGTTATAAGATGATAGATTCTAATGCATAAGTTGAGTTAAAAGATCATATAGCACTGTTAGAAGCAACTTCTGTTCTTTTGTAAATGCTTATATTATATGATATTATATTCAATTTCCCTTCATTCGTCCGTTAAGCTTTTCGGTTGCTTTTGAGTCAATCGGTGATTTAACATAGTCTAAAAGACCCTATGTTTTAACATCTATAATGTTATTTTCTCGCCAATTAATATTGATTTCTACTTTGTTGGATCTTCTACATATTTTAAATCTACAAGTGAGTGAGAGTGTTAGATGATATAATATTTTGCAACGTTAGAAACAACTTCTGTTCTATAAATGCTTATAAATCAAGCAATTTTGTTTGTTCCATTCTAATTGAAAATCAAAATTGTGTCTAACTATATCTAAGTAAGAAGCTATTAAGTATTAACAAACAAACTAGTTGGTAAACTAGTTAACAAAGTCTCTAACTCACTACATCACAATGTCTGTTTTTACTTCAATTTGATTGGATTTGATGGGTTTTCCTGTTGCTAAGGGAATGTGGTATCACCCAGTTGTGTTCTTCCATTGACTGAGGAAAATGTGGAGAAGGTGTTGGATGAAGTAAGGCCAGGCCTGATGGCTGATGGAGGGAATGTTGCTTTGCATGAGATAGATGGGCTTGTTGTGATATTGAAGCTTCAAGGAGCATGTGGTTCTTGCCCAAGCTCAACAATGACACTGAAAATGGGAATTGAGACCAGACTCAGAGACAAAATTCCTGAAATTTTGGAAGTTGAGCAGATTATGGACACTGAAACAGGACTTGAACTCAATGAGGAAAATGTTGAAAAGGTGAGTTTCTCAATTCAAGTTTCTGCTATGTAGAATGGAATTTTAAAACACTTGCATAGATGATCATAGAGTTGCTTTGCTAGATCTTAACTGACTGAAGAAGCATGCTAATTTGATTTGTTATTATATGAAAATACTACAGTATCAAATATTGCAGAATATCATGTGATAAAATAACACTGAAAATTTCTTTAACAGGGGCAGAAATTTTAGTCCCTAAGTTCTAGATATAAATGTTCTGATATATTTATAAATTCTACACTGCAGCTTATCCTTATAGCATCTTAGGTTAATGGTAATTGAACAAATGAAAACTATATTCTAGGAACAAAGTACATAAATTGATGTGGTGGTTGATTGTAGAAGTTGGAAAGTGCACATCCATTTACAACTCAATTTCAACTATATAAAAGATTAAGATCATTGTTACCATTTATCATTTACAACATCATTCACGAATTTAATTTTTACACATTAGTTGCCATGCAGTTCAAGGTTAAAAATGAAACTTTTCAAAGTCTAGAGGCATACATACTCAGTTCAAGGTTATTTGGTATAATTTGACAAAATTTTTTAAAAATATACTTTATGCATACCTCTTCCAAAGAAAAAAAAAGAAAAAGAAAAAGAAAAAGAGACAAAAACTTCATGTACTCATTTGTTTCTAGTGTTCTTGCTCGCCTCTACTAGGAAGAAAATAAACCAAAAGATGAAGGATTAACATGTATGTTGTCAACTTGAGTATTGTTCAACTGATTTAGGCAATATGCTCTCAACCAAAAGATTAGAGATTCGAAACTTCCCACATATTGTTAAACTCAGAAAGGATGAAATATGTATGTTTTTTAGGTACTTGCAGAGATTAGGCCATACTTGGCAGGCACAGGAGGTGGAATCTTGGAAGTTATTGAGATAAAAGATTATGTTGTTAAGGTGCGGCTGAGCGGACCTGCAGCTGGGGTTATGACAGTTCGAGTTGCTCTCACACAGAAGTTGAGGGAGAAGATACCAGCCATTGCAGCTGTGCAGCTTATAGAGTAG

mRNA sequence

AAAAAAAAAAAAAAAGGGAAATTGTTCAATTTATCCACATTCTTCCATAATTCCATTTCTCCTTCCCAATTTCCACCACAAAATTTGCCCACTCCATTGAAGATCTTCATCTTCATCTTCTTCACCCAATCACTATGTTTCCCTTCAGCAAAACCATAACCTAAATCCCCATTTGCAGCACACTTCCACAGCGAAATGGTTTAAGTCAACACTTCCTTCCATTTATCTCTTTATGATACTTAATAATCACTTGCATTTCAATGGTGGCCGCTTTCTCAACTCGACCTCAACCTCTTAAATCAACTCCAATCGCTGCAACTCTGTATCGCTCACCGTCTGACCCAACTTCACCTTCCCATTTGTCCCTCTTCAAGAGTTCGTTCTTTAGAGGTCAGTTTTGTAGCCAGCGCTTTCTTGGATTCAATTCAAGTTGTACTCGGAGACGCCATATAGGGAATGTGGTATCACCCAGTTGTGTTCTTCCATTGACTGAGGAAAATGTGGAGAAGGTGTTGGATGAAGTAAGGCCAGGCCTGATGGCTGATGGAGGGAATGTTGCTTTGCATGAGATAGATGGGCTTGTTGTGATATTGAAGCTTCAAGGAGCATGTGGTTCTTGCCCAAGCTCAACAATGACACTGAAAATGGGAATTGAGACCAGACTCAGAGACAAAATTCCTGAAATTTTGGAAGTTGAGCAGATTATGGACACTGAAACAGGACTTGAACTCAATGAGGAAAATGTTGAAAAGGTGCGGCTGAGCGGACCTGCAGCTGGGGTTATGACAGTTCGAGTTGCTCTCACACAGAAGTTGAGGGAGAAGATACCAGCCATTGCAGCTGTGCAGCTTATAGAGTAG

Coding sequence (CDS)

ATGGTGGCCGCTTTCTCAACTCGACCTCAACCTCTTAAATCAACTCCAATCGCTGCAACTCTGTATCGCTCACCGTCTGACCCAACTTCACCTTCCCATTTGTCCCTCTTCAAGAGTTCGTTCTTTAGAGGTCAGTTTTGTAGCCAGCGCTTTCTTGGATTCAATTCAAGTTGTACTCGGAGACGCCATATAGGGAATGTGGTATCACCCAGTTGTGTTCTTCCATTGACTGAGGAAAATGTGGAGAAGGTGTTGGATGAAGTAAGGCCAGGCCTGATGGCTGATGGAGGGAATGTTGCTTTGCATGAGATAGATGGGCTTGTTGTGATATTGAAGCTTCAAGGAGCATGTGGTTCTTGCCCAAGCTCAACAATGACACTGAAAATGGGAATTGAGACCAGACTCAGAGACAAAATTCCTGAAATTTTGGAAGTTGAGCAGATTATGGACACTGAAACAGGACTTGAACTCAATGAGGAAAATGTTGAAAAGGTGCGGCTGAGCGGACCTGCAGCTGGGGTTATGACAGTTCGAGTTGCTCTCACACAGAAGTTGAGGGAGAAGATACCAGCCATTGCAGCTGTGCAGCTTATAGAGTAG

Protein sequence

MVAAFSTRPQPLKSTPIAATLYRSPSDPTSPSHLSLFKSSFFRGQFCSQRFLGFNSSCTRRRHIGNVVSPSCVLPLTEENVEKVLDEVRPGLMADGGNVALHEIDGLVVILKLQGACGSCPSSTMTLKMGIETRLRDKIPEILEVEQIMDTETGLELNEENVEKVRLSGPAAGVMTVRVALTQKLREKIPAIAAVQLIE

Homology

[Close]

BLAST of Lsi11G007730 vs. ExPASy Swiss-Prot
Match: Q84RQ7 (NifU-like protein 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU3 PE=2 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 1.7e-52
Identity = 121/192 (63.02%), Postives = 138/192 (71.88%), Query Frame = 0


Query: 39  SSFFRGQFCSQRF--LGFNSSCTRRRHIGNVVSPSCVLPLTEENVEKVLDEVRPGLMADG 98
           S+F RGQF    F  L       +R   G+V   SCV+PLTEENVE+VLDEVRP LMADG
Sbjct: 48  STFLRGQFQRIHFSWLQHTRPLRKRTVFGHV---SCVMPLTEENVERVLDEVRPSLMADG 107

Query: 99  GNVALHEIDGLVVILKLQGACGSCPSSTMTLKMGIETRLRDKIPEILEVEQIMDTET-GL 158
           GNVALHEIDGLVV+LKLQGACGSCPSS+MTLKMGIE+RLRDKIPEI+ VEQ +++ET GL
Sbjct: 108 GNVALHEIDGLVVVLKLQGACGSCPSSSMTLKMGIESRLRDKIPEIMSVEQFLESETGGL 167

Query: 159 ELNEENVE----------------------------KVRLSGPAAGVMTVRVALTQKLRE 200
           ELN+EN+E                            KVRL+GPAAGVMTVRVALTQKLRE
Sbjct: 168 ELNDENIEKVLSELRPYLSGTGGGGLELVEIDGYVVKVRLTGPAAGVMTVRVALTQKLRE 227

[Close]

BLAST of Lsi11G007730 vs. ExPASy Swiss-Prot
Match: Q93W20 (NifU-like protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU2 PE=1 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 4.8e-47
Identity = 108/179 (60.34%), Postives = 125/179 (69.83%), Query Frame = 0


Query: 50  RFLGFNSSCTRRRHIGNVVSPSCVL--PLTEENVEKVLDEVRPGLMADGGNVALHEIDGL 109
           RFL       R + +  V +P  +L  PLTEENVE VLDE+RP LM+DGGNVALHEIDG 
Sbjct: 57  RFLSSRQLFRRSKVVKAVATPDPILEVPLTEENVESVLDEIRPYLMSDGGNVALHEIDGN 116

Query: 110 VVILKLQGACGSCPSSTMTLKMGIETRLRDKIPEILEVEQIMDTETGLELNEENVEKV-- 169
           +V +KLQGACGSCPSSTMT+KMGIE RL +KIPEI+ VE + D ETGLELNEEN+EKV  
Sbjct: 117 IVRVKLQGACGSCPSSTMTMKMGIERRLMEKIPEIVAVEALPDEETGLELNEENIEKVLE 176

Query: 170 --------------------------RLSGPAAGVMTVRVALTQKLREKIPAIAAVQLI 199
                                     R++GPAAGVMTVRVA+TQKLREKIP+IAAVQLI
Sbjct: 177 EIRPYLIGTADGSLDLVEIEDPIVKIRITGPAAGVMTVRVAVTQKLREKIPSIAAVQLI 235

[Close]

BLAST of Lsi11G007730 vs. ExPASy Swiss-Prot
Match: Q84LK7 (NifU-like protein 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=NIFU1 PE=1 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 1.7e-36
Identity = 106/227 (46.70%), Postives = 128/227 (56.39%), Query Frame = 0


Query: 14  STPIAATLYRSPSDPTSPSHLSLFKSSFFRGQFCSQRFLGFNSSCTRRRHIGNVVSPSCV 73
           + P+AA    +PS  TS S  + FK + +    C       +S  TR     N   P  V
Sbjct: 5   TVPMAAAAAVAPSTTTSSS--ASFKVAAYAWSSCRSS----SSPATRLVAAPNHQRPPLV 64

Query: 74  --------------LPLTEENVEKVLDEVRPGLMADGGNVALHEIDGLVVILKLQGACGS 133
                         LPLT  NVE VLD+VRP L ADGG+VALHEI G VV LKLQGACGS
Sbjct: 65  VGAIAGLDPVTAVQLPLTAGNVESVLDQVRPYLTADGGDVALHEIAGNVVRLKLQGACGS 124

Query: 134 CPSSTMTLKMGIETRLRDKIPEILEVEQIMDTETGLELNEENVE---------------- 193
           CPSS +T+K GIE RL +KIP++  VE + D ETGLELNEENVE                
Sbjct: 125 CPSSLITIKRGIERRLMEKIPDVAAVEPVTDKETGLELNEENVEKVLNEIRPYLAGTGGG 184

Query: 194 ------------KVRLSGPAAGVMTVRVALTQKLREKIPAIAAVQLI 199
                       KVRL+GPAA V TVR+A+++KLREKIP+I  VQL+
Sbjct: 185 GLQFLMIKGPIVKVRLTGPAAVVRTVRIAVSKKLREKIPSIQIVQLL 225

[Close]

BLAST of Lsi11G007730 vs. ExPASy Swiss-Prot
Match: P33179 (Nitrogen fixation protein NifU (Fragment) OS=Anabaena sp. (strain L31) OX=29412 GN=nifU PE=3 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 1.7e-15
Identity = 43/69 (62.32%), Postives = 56/69 (81.16%), Query Frame = 0


Query: 81  VEKVLD-EVRPGLMADGGNVALHEIDGLVVILKLQGACGSCPSSTMTLKMGIETRLRDKI 140
           ++KVLD EVRP L+ADGG+V L+++DG +V + LQGACGSCPSST TLK+ IE+RLRD+I
Sbjct: 44  IQKVLDEEVRPVLIADGGDVELYDVDGDIVKVVLQGACGSCPSSTATLKIAIESRLRDRI 103

Query: 141 PEILEVEQI 149
              L VE +
Sbjct: 104 NPSLVVEAV 112

[Close]

BLAST of Lsi11G007730 vs. ExPASy Swiss-Prot
Match: O32119 (Putative nitrogen fixation protein YutI OS=Bacillus subtilis (strain 168) OX=224308 GN=yutI PE=3 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 8.2e-15
Identity = 38/71 (53.52%), Postives = 56/71 (78.87%), Query Frame = 0


Query: 78  EENVEKVLDEVRPGLMADGGNVALHEIDGLVVILKLQGACGSCPSSTMTLKMGIETRLRD 137
           +E V++VLD++RP L+ DGG+  L ++D  +V L+L GACGSCPSST+TLK GIE  L +
Sbjct: 40  KEQVQEVLDKLRPFLLRDGGDCELVDVDEGIVKLRLLGACGSCPSSTITLKAGIERALLE 99

Query: 138 KIPEILEVEQI 149
           ++P ++EVEQ+
Sbjct: 100 EVPGVVEVEQV 110

[Close]

BLAST of Lsi11G007730 vs. ExPASy TrEMBL
Match: A0A0A0LMF5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G060370 PE=3 SV=1)

HSP 1 Score: 343.2 bits (879), Expect = 7.3e-91
Identity = 187/227 (82.38%), Postives = 190/227 (83.70%), Query Frame = 0


Query: 1   MVAAFSTRPQPLKSTPIAATLYRSPSDPTSPSHLSLFKSSFFRGQFCSQRFLGFNSSCTR 60
           MVA FSTR QP KSTPIAATLYRSPS P SPS+LS FKSSFFRGQFCS+ FL FNSSCTR
Sbjct: 1   MVATFSTRAQPFKSTPIAATLYRSPSHPASPSYLSFFKSSFFRGQFCSRHFLRFNSSCTR 60

Query: 61  RRHIGNVVSPSCVLPLTEENVEKVLDEVRPGLMADGGNVALHEIDGLVVILKLQGACGSC 120
            RH+GNVVSPSCVLPLTEENVEKVLDEVRPGLMADGGNVALHEIDGLVVILKLQGACGSC
Sbjct: 61  IRHLGNVVSPSCVLPLTEENVEKVLDEVRPGLMADGGNVALHEIDGLVVILKLQGACGSC 120

Query: 121 PSSTMTLKMGIETRLRDKIPEILEVEQIMDTETGLELNEENVE----------------- 180
           PSSTMTLKMGIETRLRDKIPEILEVEQIMDTETGLELNEENVE                 
Sbjct: 121 PSSTMTLKMGIETRLRDKIPEILEVEQIMDTETGLELNEENVEKVLSEIRPYLAGTGGGI 180

Query: 181 -----------KVRLSGPAAGVMTVRVALTQKLREKIPAIAAVQLIE 200
                      KVRLSGPAAGVMTVRVALTQKLREKIPAIAAVQLIE
Sbjct: 181 LEVIEIKDYVVKVRLSGPAAGVMTVRVALTQKLREKIPAIAAVQLIE 227

[Close]

BLAST of Lsi11G007730 vs. ExPASy TrEMBL
Match: A0A5D3BPX3 (NifU-like protein 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold29G00910 PE=3 SV=1)

HSP 1 Score: 337.8 bits (865), Expect = 3.1e-89
Identity = 186/227 (81.94%), Postives = 188/227 (82.82%), Query Frame = 0


Query: 1   MVAAFSTRPQPLKSTPIAATLYRSPSDPTSPSHLSLFKSSFFRGQFCSQRFLGFNSSCTR 60
           MVA FSTR QP KSTPIAATLY S S PTSPSHLSLFKSSFFRGQFCS+RFL FN SCTR
Sbjct: 1   MVATFSTRAQPFKSTPIAATLYPSSSHPTSPSHLSLFKSSFFRGQFCSRRFLRFNPSCTR 60

Query: 61  RRHIGNVVSPSCVLPLTEENVEKVLDEVRPGLMADGGNVALHEIDGLVVILKLQGACGSC 120
            R +GNVVSPSCVLPLTEENVEKVLDEVRP LMADGGNVALHEIDGLVVILKLQGACGSC
Sbjct: 61  IRRLGNVVSPSCVLPLTEENVEKVLDEVRPSLMADGGNVALHEIDGLVVILKLQGACGSC 120

Query: 121 PSSTMTLKMGIETRLRDKIPEILEVEQIMDTETGLELNEENVE----------------- 180
           PSSTMTLKMGIETRLRDKIPEILEVEQIMDTETGLELNEENVE                 
Sbjct: 121 PSSTMTLKMGIETRLRDKIPEILEVEQIMDTETGLELNEENVEKVLSEIRPYLAGTGGGI 180

Query: 181 -----------KVRLSGPAAGVMTVRVALTQKLREKIPAIAAVQLIE 200
                      KVRLSGPAAGVMTVRVALTQKLREKIPAIAAVQLIE
Sbjct: 181 LEVIEIKDYVVKVRLSGPAAGVMTVRVALTQKLREKIPAIAAVQLIE 227

[Close]

BLAST of Lsi11G007730 vs. ExPASy TrEMBL
Match: A0A6J1EP39 (nifU-like protein 3, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111436107 PE=3 SV=1)

HSP 1 Score: 337.8 bits (865), Expect = 3.1e-89
Identity = 184/227 (81.06%), Postives = 189/227 (83.26%), Query Frame = 0


Query: 1   MVAAFSTRPQPLKSTPIAATLYRSPSDPTSPSHLSLFKSSFFRGQFCSQRFLGFNSSCTR 60
           MVAAFSTR Q LK TP  ATLYRSPS+ TS SHLS FKSSFFRGQFCS+RFLGFNSSCTR
Sbjct: 1   MVAAFSTRAQALKPTPTTATLYRSPSERTSASHLSFFKSSFFRGQFCSRRFLGFNSSCTR 60

Query: 61  RRHIGNVVSPSCVLPLTEENVEKVLDEVRPGLMADGGNVALHEIDGLVVILKLQGACGSC 120
           RRH+GNVVSPSCVLPLTEENVEKVLDEVRP LMADGGNVALHEIDGLVVILKLQGACGSC
Sbjct: 61  RRHLGNVVSPSCVLPLTEENVEKVLDEVRPSLMADGGNVALHEIDGLVVILKLQGACGSC 120

Query: 121 PSSTMTLKMGIETRLRDKIPEILEVEQIMDTETGLELNEENVE----------------- 180
           PSSTMTLKMGIETRLRDKIPEILEVEQI+DTETGLELN +NVE                 
Sbjct: 121 PSSTMTLKMGIETRLRDKIPEILEVEQIIDTETGLELNHDNVEKLLAEIRPYLAGTGGGI 180

Query: 181 -----------KVRLSGPAAGVMTVRVALTQKLREKIPAIAAVQLIE 200
                      KVRLSGPAAGVMTVRVALTQKLREKIPAIAAVQLIE
Sbjct: 181 LELIEIKDYVVKVRLSGPAAGVMTVRVALTQKLREKIPAIAAVQLIE 227

[Close]

BLAST of Lsi11G007730 vs. ExPASy TrEMBL
Match: A0A5A7U598 (NifU-like protein 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1484G00030 PE=3 SV=1)

HSP 1 Score: 337.4 bits (864), Expect = 4.0e-89
Identity = 185/227 (81.50%), Postives = 188/227 (82.82%), Query Frame = 0


Query: 1   MVAAFSTRPQPLKSTPIAATLYRSPSDPTSPSHLSLFKSSFFRGQFCSQRFLGFNSSCTR 60
           MVA FSTR QP KSTPIAATLY S S PTSPSHLSLFKSSFFRGQFCS+RFL FN SCTR
Sbjct: 1   MVATFSTRAQPFKSTPIAATLYPSSSHPTSPSHLSLFKSSFFRGQFCSRRFLRFNPSCTR 60

Query: 61  RRHIGNVVSPSCVLPLTEENVEKVLDEVRPGLMADGGNVALHEIDGLVVILKLQGACGSC 120
            R +GNV+SPSCVLPLTEENVEKVLDEVRP LMADGGNVALHEIDGLVVILKLQGACGSC
Sbjct: 61  IRRLGNVISPSCVLPLTEENVEKVLDEVRPSLMADGGNVALHEIDGLVVILKLQGACGSC 120

Query: 121 PSSTMTLKMGIETRLRDKIPEILEVEQIMDTETGLELNEENVE----------------- 180
           PSSTMTLKMGIETRLRDKIPEILEVEQIMDTETGLELNEENVE                 
Sbjct: 121 PSSTMTLKMGIETRLRDKIPEILEVEQIMDTETGLELNEENVEKVLSEIRPYLAGTGGGI 180

Query: 181 -----------KVRLSGPAAGVMTVRVALTQKLREKIPAIAAVQLIE 200
                      KVRLSGPAAGVMTVRVALTQKLREKIPAIAAVQLIE
Sbjct: 181 LEVIEIKDYVVKVRLSGPAAGVMTVRVALTQKLREKIPAIAAVQLIE 227

[Close]

BLAST of Lsi11G007730 vs. ExPASy TrEMBL
Match: A0A1S3BRP8 (nifU-like protein 3, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492775 PE=3 SV=1)

HSP 1 Score: 337.4 bits (864), Expect = 4.0e-89
Identity = 185/227 (81.50%), Postives = 188/227 (82.82%), Query Frame = 0


Query: 1   MVAAFSTRPQPLKSTPIAATLYRSPSDPTSPSHLSLFKSSFFRGQFCSQRFLGFNSSCTR 60
           MVA FSTR QP KSTPIAATLY S S PTSPSHLSLFKSSFFRGQFCS+RFL FN SCTR
Sbjct: 1   MVATFSTRAQPFKSTPIAATLYPSSSHPTSPSHLSLFKSSFFRGQFCSRRFLRFNPSCTR 60

Query: 61  RRHIGNVVSPSCVLPLTEENVEKVLDEVRPGLMADGGNVALHEIDGLVVILKLQGACGSC 120
            R +GNV+SPSCVLPLTEENVEKVLDEVRP LMADGGNVALHEIDGLVVILKLQGACGSC
Sbjct: 61  IRRLGNVISPSCVLPLTEENVEKVLDEVRPSLMADGGNVALHEIDGLVVILKLQGACGSC 120

Query: 121 PSSTMTLKMGIETRLRDKIPEILEVEQIMDTETGLELNEENVE----------------- 180
           PSSTMTLKMGIETRLRDKIPEILEVEQIMDTETGLELNEENVE                 
Sbjct: 121 PSSTMTLKMGIETRLRDKIPEILEVEQIMDTETGLELNEENVEKVLSEIRPYLAGTGGGI 180

Query: 181 -----------KVRLSGPAAGVMTVRVALTQKLREKIPAIAAVQLIE 200
                      KVRLSGPAAGVMTVRVALTQKLREKIPAIAAVQLIE
Sbjct: 181 LEVIEIKDYVVKVRLSGPAAGVMTVRVALTQKLREKIPAIAAVQLIE 227

[Close]

BLAST of Lsi11G007730 vs. NCBI nr
Match: XP_038889481.1 (nifU-like protein 3, chloroplastic [Benincasa hispida])

HSP 1 Score: 348.2 bits (892), Expect = 4.7e-92
Identity = 189/227 (83.26%), Postives = 194/227 (85.46%), Query Frame = 0


Query: 1   MVAAFSTRPQPLKSTPIAATLYRSPSDPTSPSHLSLFKSSFFRGQFCSQRFLGFNSSCTR 60
           MVAAFSTR  PLKSTPIAATLY SPS+PTSPSHLSLFK+SFFRGQFCS+RFL FNSSCT+
Sbjct: 1   MVAAFSTRAHPLKSTPIAATLYCSPSEPTSPSHLSLFKTSFFRGQFCSRRFLRFNSSCTQ 60

Query: 61  RRHIGNVVSPSCVLPLTEENVEKVLDEVRPGLMADGGNVALHEIDGLVVILKLQGACGSC 120
           RRH+GNVVSPSCVLPLTEENVEKVLDEVRP LMADGGNVALHEIDGLVVILKLQGACGSC
Sbjct: 61  RRHLGNVVSPSCVLPLTEENVEKVLDEVRPSLMADGGNVALHEIDGLVVILKLQGACGSC 120

Query: 121 PSSTMTLKMGIETRLRDKIPEILEVEQIMDTETGLELNEENVE----------------- 180
           PSSTMTLKMGIETRLRDKIPEILEVEQIMDTETGLELNEENVE                 
Sbjct: 121 PSSTMTLKMGIETRLRDKIPEILEVEQIMDTETGLELNEENVEKVLAEIRPYLAGTGGGI 180

Query: 181 -----------KVRLSGPAAGVMTVRVALTQKLREKIPAIAAVQLIE 200
                      KVRLSGPAAGVMTVRVALTQKLREKIPAIAAVQLIE
Sbjct: 181 LEVIEIKDYVVKVRLSGPAAGVMTVRVALTQKLREKIPAIAAVQLIE 227

[Close]

BLAST of Lsi11G007730 vs. NCBI nr
Match: XP_004148324.1 (nifU-like protein 3, chloroplastic isoform X2 [Cucumis sativus] >KGN61156.1 hypothetical protein Csa_021276 [Cucumis sativus])

HSP 1 Score: 343.2 bits (879), Expect = 1.5e-90
Identity = 187/227 (82.38%), Postives = 190/227 (83.70%), Query Frame = 0


Query: 1   MVAAFSTRPQPLKSTPIAATLYRSPSDPTSPSHLSLFKSSFFRGQFCSQRFLGFNSSCTR 60
           MVA FSTR QP KSTPIAATLYRSPS P SPS+LS FKSSFFRGQFCS+ FL FNSSCTR
Sbjct: 1   MVATFSTRAQPFKSTPIAATLYRSPSHPASPSYLSFFKSSFFRGQFCSRHFLRFNSSCTR 60

Query: 61  RRHIGNVVSPSCVLPLTEENVEKVLDEVRPGLMADGGNVALHEIDGLVVILKLQGACGSC 120
            RH+GNVVSPSCVLPLTEENVEKVLDEVRPGLMADGGNVALHEIDGLVVILKLQGACGSC
Sbjct: 61  IRHLGNVVSPSCVLPLTEENVEKVLDEVRPGLMADGGNVALHEIDGLVVILKLQGACGSC 120

Query: 121 PSSTMTLKMGIETRLRDKIPEILEVEQIMDTETGLELNEENVE----------------- 180
           PSSTMTLKMGIETRLRDKIPEILEVEQIMDTETGLELNEENVE                 
Sbjct: 121 PSSTMTLKMGIETRLRDKIPEILEVEQIMDTETGLELNEENVEKVLSEIRPYLAGTGGGI 180

Query: 181 -----------KVRLSGPAAGVMTVRVALTQKLREKIPAIAAVQLIE 200
                      KVRLSGPAAGVMTVRVALTQKLREKIPAIAAVQLIE
Sbjct: 181 LEVIEIKDYVVKVRLSGPAAGVMTVRVALTQKLREKIPAIAAVQLIE 227

[Close]

BLAST of Lsi11G007730 vs. NCBI nr
Match: XP_031737073.1 (nifU-like protein 3, chloroplastic isoform X1 [Cucumis sativus])

HSP 1 Score: 341.3 bits (874), Expect = 5.7e-90
Identity = 187/232 (80.60%), Postives = 190/232 (81.90%), Query Frame = 0


Query: 1   MVAAFSTRPQPLKSTPIAATLYRSPSDPTSPSHLSLFKSSFFRGQFCSQRFLGFNSSCTR 60
           MVA FSTR QP KSTPIAATLYRSPS P SPS+LS FKSSFFRGQFCS+ FL FNSSCTR
Sbjct: 1   MVATFSTRAQPFKSTPIAATLYRSPSHPASPSYLSFFKSSFFRGQFCSRHFLRFNSSCTR 60

Query: 61  RRHIGNVVSPSCVLPLTEENVEKVLDEVRPGLMADGGNVALHEIDGLVVILKLQGACGSC 120
            RH+GNVVSPSCVLPLTEENVEKVLDEVRPGLMADGGNVALHEIDGLVVILKLQGACGSC
Sbjct: 61  IRHLGNVVSPSCVLPLTEENVEKVLDEVRPGLMADGGNVALHEIDGLVVILKLQGACGSC 120

Query: 121 PSSTMTLKMGIETRLRDKIPEILEVEQIMDTETGLELNEENVE----------------- 180
           PSSTMTLKMGIETRLRDKIPEILEVEQIMDTETGLELNEENVE                 
Sbjct: 121 PSSTMTLKMGIETRLRDKIPEILEVEQIMDTETGLELNEENVEKNETYQVLSEIRPYLAG 180

Query: 181 ----------------KVRLSGPAAGVMTVRVALTQKLREKIPAIAAVQLIE 200
                           KVRLSGPAAGVMTVRVALTQKLREKIPAIAAVQLIE
Sbjct: 181 TGGGILEVIEIKDYVVKVRLSGPAAGVMTVRVALTQKLREKIPAIAAVQLIE 232

[Close]

BLAST of Lsi11G007730 vs. NCBI nr
Match: XP_022929559.1 (nifU-like protein 3, chloroplastic [Cucurbita moschata] >KAG6598879.1 NifU-like protein 3, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 337.8 bits (865), Expect = 6.3e-89
Identity = 184/227 (81.06%), Postives = 189/227 (83.26%), Query Frame = 0


Query: 1   MVAAFSTRPQPLKSTPIAATLYRSPSDPTSPSHLSLFKSSFFRGQFCSQRFLGFNSSCTR 60
           MVAAFSTR Q LK TP  ATLYRSPS+ TS SHLS FKSSFFRGQFCS+RFLGFNSSCTR
Sbjct: 1   MVAAFSTRAQALKPTPTTATLYRSPSERTSASHLSFFKSSFFRGQFCSRRFLGFNSSCTR 60

Query: 61  RRHIGNVVSPSCVLPLTEENVEKVLDEVRPGLMADGGNVALHEIDGLVVILKLQGACGSC 120
           RRH+GNVVSPSCVLPLTEENVEKVLDEVRP LMADGGNVALHEIDGLVVILKLQGACGSC
Sbjct: 61  RRHLGNVVSPSCVLPLTEENVEKVLDEVRPSLMADGGNVALHEIDGLVVILKLQGACGSC 120

Query: 121 PSSTMTLKMGIETRLRDKIPEILEVEQIMDTETGLELNEENVE----------------- 180
           PSSTMTLKMGIETRLRDKIPEILEVEQI+DTETGLELN +NVE                 
Sbjct: 121 PSSTMTLKMGIETRLRDKIPEILEVEQIIDTETGLELNHDNVEKLLAEIRPYLAGTGGGI 180

Query: 181 -----------KVRLSGPAAGVMTVRVALTQKLREKIPAIAAVQLIE 200
                      KVRLSGPAAGVMTVRVALTQKLREKIPAIAAVQLIE
Sbjct: 181 LELIEIKDYVVKVRLSGPAAGVMTVRVALTQKLREKIPAIAAVQLIE 227

[Close]

BLAST of Lsi11G007730 vs. NCBI nr
Match: TYK01397.1 (nifU-like protein 3 [Cucumis melo var. makuwa])

HSP 1 Score: 337.8 bits (865), Expect = 6.3e-89
Identity = 186/227 (81.94%), Postives = 188/227 (82.82%), Query Frame = 0


Query: 1   MVAAFSTRPQPLKSTPIAATLYRSPSDPTSPSHLSLFKSSFFRGQFCSQRFLGFNSSCTR 60
           MVA FSTR QP KSTPIAATLY S S PTSPSHLSLFKSSFFRGQFCS+RFL FN SCTR
Sbjct: 1   MVATFSTRAQPFKSTPIAATLYPSSSHPTSPSHLSLFKSSFFRGQFCSRRFLRFNPSCTR 60

Query: 61  RRHIGNVVSPSCVLPLTEENVEKVLDEVRPGLMADGGNVALHEIDGLVVILKLQGACGSC 120
            R +GNVVSPSCVLPLTEENVEKVLDEVRP LMADGGNVALHEIDGLVVILKLQGACGSC
Sbjct: 61  IRRLGNVVSPSCVLPLTEENVEKVLDEVRPSLMADGGNVALHEIDGLVVILKLQGACGSC 120

Query: 121 PSSTMTLKMGIETRLRDKIPEILEVEQIMDTETGLELNEENVE----------------- 180
           PSSTMTLKMGIETRLRDKIPEILEVEQIMDTETGLELNEENVE                 
Sbjct: 121 PSSTMTLKMGIETRLRDKIPEILEVEQIMDTETGLELNEENVEKVLSEIRPYLAGTGGGI 180

Query: 181 -----------KVRLSGPAAGVMTVRVALTQKLREKIPAIAAVQLIE 200
                      KVRLSGPAAGVMTVRVALTQKLREKIPAIAAVQLIE
Sbjct: 181 LEVIEIKDYVVKVRLSGPAAGVMTVRVALTQKLREKIPAIAAVQLIE 227

[Close]

BLAST of Lsi11G007730 vs. TAIR 10
Match: AT4G25910.1 (NFU domain protein 3 )

HSP 1 Score: 207.2 bits (526), Expect = 1.2e-53
Identity = 121/192 (63.02%), Postives = 138/192 (71.88%), Query Frame = 0


Query: 39  SSFFRGQFCSQRF--LGFNSSCTRRRHIGNVVSPSCVLPLTEENVEKVLDEVRPGLMADG 98
           S+F RGQF    F  L       +R   G+V   SCV+PLTEENVE+VLDEVRP LMADG
Sbjct: 48  STFLRGQFQRIHFSWLQHTRPLRKRTVFGHV---SCVMPLTEENVERVLDEVRPSLMADG 107

Query: 99  GNVALHEIDGLVVILKLQGACGSCPSSTMTLKMGIETRLRDKIPEILEVEQIMDTET-GL 158
           GNVALHEIDGLVV+LKLQGACGSCPSS+MTLKMGIE+RLRDKIPEI+ VEQ +++ET GL
Sbjct: 108 GNVALHEIDGLVVVLKLQGACGSCPSSSMTLKMGIESRLRDKIPEIMSVEQFLESETGGL 167

Query: 159 ELNEENVE----------------------------KVRLSGPAAGVMTVRVALTQKLRE 200
           ELN+EN+E                            KVRL+GPAAGVMTVRVALTQKLRE
Sbjct: 168 ELNDENIEKVLSELRPYLSGTGGGGLELVEIDGYVVKVRLTGPAAGVMTVRVALTQKLRE 227

[Close]

BLAST of Lsi11G007730 vs. TAIR 10
Match: AT5G49940.1 (NIFU-like protein 2 )

HSP 1 Score: 189.1 bits (479), Expect = 3.4e-48
Identity = 108/179 (60.34%), Postives = 125/179 (69.83%), Query Frame = 0


Query: 50  RFLGFNSSCTRRRHIGNVVSPSCVL--PLTEENVEKVLDEVRPGLMADGGNVALHEIDGL 109
           RFL       R + +  V +P  +L  PLTEENVE VLDE+RP LM+DGGNVALHEIDG 
Sbjct: 57  RFLSSRQLFRRSKVVKAVATPDPILEVPLTEENVESVLDEIRPYLMSDGGNVALHEIDGN 116

Query: 110 VVILKLQGACGSCPSSTMTLKMGIETRLRDKIPEILEVEQIMDTETGLELNEENVEKV-- 169
           +V +KLQGACGSCPSSTMT+KMGIE RL +KIPEI+ VE + D ETGLELNEEN+EKV  
Sbjct: 117 IVRVKLQGACGSCPSSTMTMKMGIERRLMEKIPEIVAVEALPDEETGLELNEENIEKVLE 176

Query: 170 --------------------------RLSGPAAGVMTVRVALTQKLREKIPAIAAVQLI 199
                                     R++GPAAGVMTVRVA+TQKLREKIP+IAAVQLI
Sbjct: 177 EIRPYLIGTADGSLDLVEIEDPIVKIRITGPAAGVMTVRVAVTQKLREKIPSIAAVQLI 235

[Close]

BLAST of Lsi11G007730 vs. TAIR 10
Match: AT5G49940.2 (NIFU-like protein 2 )

HSP 1 Score: 152.9 bits (385), Expect = 2.7e-37
Identity = 79/119 (66.39%), Postives = 93/119 (78.15%), Query Frame = 0


Query: 50  RFLGFNSSCTRRRHIGNVVSPSCVL--PLTEENVEKVLDEVRPGLMADGGNVALHEIDGL 109
           RFL       R + +  V +P  +L  PLTEENVE VLDE+RP LM+DGGNVALHEIDG 
Sbjct: 57  RFLSSRQLFRRSKVVKAVATPDPILEVPLTEENVESVLDEIRPYLMSDGGNVALHEIDGN 116

Query: 110 VVILKLQGACGSCPSSTMTLKMGIETRLRDKIPEILEVEQIMDTETGLELNEENVEKVR 167
           +V +KLQGACGSCPSSTMT+KMGIE RL +KIPEI+ VE + D ETGLELNEEN+EKV+
Sbjct: 117 IVRVKLQGACGSCPSSTMTMKMGIERRLMEKIPEIVAVEALPDEETGLELNEENIEKVK 175

[Close]

BLAST of Lsi11G007730 vs. TAIR 10
Match: AT4G01940.1 (NFU domain protein 1 )

HSP 1 Score: 78.6 bits (192), Expect = 6.4e-15
Identity = 40/78 (51.28%), Postives = 57/78 (73.08%), Query Frame = 0


Query: 76  LTEENVEKVLDEVRPGLMADGGNVALHEIDGLVVILKLQGACGSCPSSTMTLKMGIETRL 135
           LT +NV+ VL++VRP L++DGGNV +  ++  VV LKLQGAC SCPSS+ T+ MGIE  L
Sbjct: 85  LTPQNVDLVLEDVRPFLISDGGNVDVVSVEDGVVSLKLQGACTSCPSSSTTMTMGIERVL 144

Query: 136 RDKIPEIL-EVEQIMDTE 153
           ++K  + L ++ Q+ D E
Sbjct: 145 KEKFGDALKDIRQVFDEE 162

[Close]

BLAST of Lsi11G007730 vs. TAIR 10
Match: AT3G20970.1 (NFU domain protein 4 )

HSP 1 Score: 55.1 bits (131), Expect = 7.6e-08
Identity = 29/67 (43.28%), Postives = 40/67 (59.70%), Query Frame = 0


Query: 88  VRPGLMADGGNVALHEID--GLVVILKLQGACGSCPSSTMTLKMGIETRLRDKIPEILEV 147
           +RP +  DGG++     D    +V L++QGAC  CPSS++TLK GIE  L   + E+  V
Sbjct: 204 IRPAVQDDGGDIEYCGFDPESGIVKLRMQGACSGCPSSSVTLKSGIENMLMHYVSEVKGV 263

Query: 148 EQIMDTE 153
           EQ  D E
Sbjct: 264 EQEFDGE 270

The following BLAST results are available for this feature:

BLAST of Lsi11G007730 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84RQ7	1.7e-52	63.02	NifU-like protein 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU3 PE=2...	[more]
Q93W20	4.8e-47	60.34	NifU-like protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU2 PE=1...	[more]
Q84LK7	1.7e-36	46.70	NifU-like protein 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=N...	[more]
P33179	1.7e-15	62.32	Nitrogen fixation protein NifU (Fragment) OS=Anabaena sp. (strain L31) OX=29412 ...	[more]
O32119	8.2e-15	53.52	Putative nitrogen fixation protein YutI OS=Bacillus subtilis (strain 168) OX=224...	[more]

BLAST of Lsi11G007730 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LMF5	7.3e-91	82.38	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G060370 PE=3 SV=1	[more]
A0A5D3BPX3	3.1e-89	81.94	NifU-like protein 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold29G0...	[more]
A0A6J1EP39	3.1e-89	81.06	nifU-like protein 3, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111436107...	[more]
A0A5A7U598	4.0e-89	81.50	NifU-like protein 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1484...	[more]
A0A1S3BRP8	4.0e-89	81.50	nifU-like protein 3, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492775 PE=3 ...	[more]

BLAST of Lsi11G007730 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038889481.1	4.7e-92	83.26	nifU-like protein 3, chloroplastic [Benincasa hispida]	[more]
XP_004148324.1	1.5e-90	82.38	nifU-like protein 3, chloroplastic isoform X2 [Cucumis sativus] >KGN61156.1 hypo...	[more]
XP_031737073.1	5.7e-90	80.60	nifU-like protein 3, chloroplastic isoform X1 [Cucumis sativus]	[more]
XP_022929559.1	6.3e-89	81.06	nifU-like protein 3, chloroplastic [Cucurbita moschata] >KAG6598879.1 NifU-like ...	[more]
TYK01397.1	6.3e-89	81.94	nifU-like protein 3 [Cucumis melo var. makuwa]	[more]

BLAST of Lsi11G007730 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G25910.1	1.2e-53	63.02	NFU domain protein 3	[more]
AT5G49940.1	3.4e-48	60.34	NIFU-like protein 2	[more]
AT5G49940.2	2.7e-37	66.39	NIFU-like protein 2	[more]
AT4G01940.1	6.4e-15	51.28	NFU domain protein 1	[more]
AT3G20970.1	7.6e-08	43.28	NFU domain protein 4	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001075	NIF system FeS cluster assembly, NifU, C-terminal	PFAM	PF01106	NifU	coord: 81..146 e-value: 2.5E-26 score: 91.6
IPR034904	Fe-S cluster assembly domain superfamily	GENE3D	3.30.300.130		coord: 73..156 e-value: 1.8E-32 score: 113.2
IPR034904	Fe-S cluster assembly domain superfamily	GENE3D	3.30.300.130		coord: 157..199 e-value: 6.3E-6 score: 28.2
IPR034904	Fe-S cluster assembly domain superfamily	SUPERFAMILY	117916	Fe-S cluster assembly (FSCA) domain-like	coord: 78..153
None	No IPR available	PANTHER	PTHR11178	IRON-SULFUR CLUSTER SCAFFOLD PROTEIN NFU-RELATED	coord: 30..165
None	No IPR available	PANTHER	PTHR11178:SF25	NIFU-LIKE PROTEIN 3, CHLOROPLASTIC	coord: 164..199
None	No IPR available	PANTHER	PTHR11178:SF25	NIFU-LIKE PROTEIN 3, CHLOROPLASTIC	coord: 30..165
None	No IPR available	PANTHER	PTHR11178	IRON-SULFUR CLUSTER SCAFFOLD PROTEIN NFU-RELATED	coord: 164..199

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi11G007730.1	Lsi11G007730.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016226	iron-sulfur cluster assembly
biological_process	GO:0097428	protein maturation by iron-sulfur cluster transfer
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0051539	4 iron, 4 sulfur cluster binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0051536	iron-sulfur cluster binding