Lsi11G007460 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi11G007460
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description(R)-mandelonitrile lyase 1-like
Locationchr11: 8228058 .. 8235161 (-)
RNA-Seq ExpressionLsi11G007460
SyntenyLsi11G007460
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGGTGAACATAATAGTCCAACAAACTTGTGCAATTGTCTAGATGAAAGTCAAATTGTTTAGGCCCGATTCACAATTATTTGAGTTTTTTATTTTTTGGTATGAAGATTTAGTCAACCAACACGACTTCTACCTATTAATTTTTTTTCTATATTTTAAAAGTGTTTTTAAAATTCAAGTGAATGTTAATTCTTCAGAGCAGTCTCTATTTAAAGTTTTTCTTTCTTTTTTGAAAAAGACAAAACTGTGACCGATTAAAGTATGATCACAGATACATTAGATTGAGGATAATAATAATGAAGAAAAAAGAAGGAGAGAATAACACACAAAATTTTTAAGTAGATATCTCTACACAAATTAGGATAAAAATCAGGAGCAGAGATAAAAGAATTATTTTGTATAAAATTGAAGTTTAATTACAAACTTTCTTTGTTAGAAAGGAAGAACAAACTCTCTTATAAGAAGAAACTATTTCTATTTTATCTCACAACTCACTTGATTAGTAATTTGGGATGATTGTGTGTACCCACCTATTTATAGTCTACATGAACATGGCCATCAAACAAATTCAATGACTCAGTCCAATTGTCTTTGATAAATGTGAAAGATATAACGGTCACAAATTGACCTTATACAAAGTGAAAGATCTAATGGCTAGAAATTAATCAATGGCCTAATTTTCCTTTCAAGAAGTTTCTTGAATAATCTTGAAGATAATATTATTTAACAAAAACTTCTAATATAAAAAAAAAATATTTCAAAGGGAAATTATTTTAAGTGACCAAAATGCTAAAAATATTTACAAATATAGCAAAATTTCACTGTCTATCTGCGATAGATCGCGATAGACTATTATTTGTGTCACGATAACTACAGATAGACACAGATAGTAGTCTATCACGATCTATCGCAGATAGATAGTGAAATTTTGCTATATTTGTAATATTTTGGTTCATATTCTTATATTTGAAAAGAGTCCGATTTCAAATACATTTATCTAATTATTCATACATATTGTTGAAAACATACCATCACAAGCCCATAATAGATACAAATTATCAAATTGATTATACAATAAAACTTTATATCTATATATATAAGAAATTAAACGAGAACACTATGAGAATAAGGACAAAGAATATAGAGCTACACCTATTCTCATACTTCCATTTCTTTTACTCCAAAAAAAAATTTGCAACTTTTTTCAAGAATATATTTTTCATCTCCATCTTTATTTATCTCACTAAAACATTTTGCCCAATCCTAATTCACTCCTCGAAACAGGACGACCACGTGGAGAAAATGAATATTATTAAACTTCATCATCATTTGTTTTATTTTATTATGTGACATTAAAACTCAAACTAAATTCTCTTTATACTTTTCTTATCCTACGTCAATCTTCTTACTCAAACTTGTCCACATTTCACAATTAGTCAAAGTTTGGTAATAAATTTTTCAACCAACATGTGCAATAATGTTCTCTAATGTTTCTGAAACAGACGTGTCTATTGGTTCATTGACAAATTTGTAAAAATACACATTAAGCATAAACTCCATCCCCAACATTTTTCTTTAAGAACTTTTCAACCGTCAACGACCCAATTTGAATATTATATATTTTTCAAGTCTTTTGTAAGTTAAATGGTTCCACTTTTTTATTTTGTATTAGCCATCGTTAACCCTTCGCATGCCATATCTTCGTATATGAAATAAACCACAAAATATTTCAAATTGGCAAGTTGTGTTTTGTAAAGTTGACCCATTGACCAAATCTAGCTTCAATTTCTGTATATAAAAAGAATAATGTTACCATCATTTGAGCATAACCTCTCTCCTTCTCTAAGAATTGATAGGATTCATCAATGGCTTCTCTTTTCCTACTCATACTTACTCTATTTACCTTTCATGTTCAAATTGGAGTTTTCTCCTCACAAACTATACCAAATCAAGGTATTTCTTGTTTTTATCTCTCTCATTGGAGCGTTCTTATGGAGTAGAGAGCTATATATCTTTTCTAGGGGCAAACAAAATTCCTGGTTTGAAAACTATTTGAATTTTAGTTTTTGGTTTTTGAAAATCATGGTTATAAATGTTATTTTCACCGATAAGTTTCTTTTGTTTTGTTATTCACTCTCTTTATTGATATTTTCAAAGTCCAAGTCAAGCTTTGGAAACTAAAAAAATAATTTTTATAAACTTGTCTTTGTTTTTCGAGATTAAATATTTTAATGTTGTTTAAAAAAGGTGAAAACCATGGTAAGGAAATTTAGAGAACACATACAAATATGAATTTGAAAAACAAAAAATAAAAAATGAGATTGCTACTGAACGGTGTTTTAGCTTCTAATTTTTCATGTATACATTTTCATCCTTAAAATTTTTCAATTTGTGACAAATAGATATCTCTAAATTTTAAATTTAGTCTCTAATTAAAAGTATGTACTAGTCATACAAATACAATTGAAAGTATAAGATCTCATTAGAGATAAAGTTCAATTTTACCTCTATTATATCATTTAATTTATTAAAATTCTGAAAATGTATGAAATTTACAAGATACCAAATTGAAAGTTGAGACTTCATTAGACACAAAATTGAAAGTTTAAAAACTCGTTAGATACTTTTTAAACTTAAGGGGTCAAGTAGACACAAATTTAAAAGTTAAGTAACCAAACTTGTAATTTGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTATTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATCTTTCTAACATAAGATATCTAAATAACAATTGATAGCTGCCCCAATCCTTTTTTTTTTTTTCAAGCTTGTCTTAATATTTTCTCTCTAACTACTAATCCTCCAATCTTTTCTTTTAGATGATGGTTACACAAAGTTTGTACAAAACGCCAATACTTTACCAACAACAAAAGAATACGACTATATAATAGTAGGAGGAGGGACGGCCGGTTGCCCATTAGCTGCAACATTATCATCAAATTTCTCTGTTCTTGTTCTTGAAAGAGGAAGTGACCCTAATGCCTTCCCCTTGGTCCTATCCCAAGAAGGCATGGGAAACACTCTCACCGACGATGACGATGGCAGCAACCCATTCCAGCGATTTGTTTCTGAAGACGGTGTCGAGAACATTAGAGGTCGAGTCCTCGGTGGAGGTAGCATGATTAACGTCGGGTTCTACTCGAGAGCTCAGCCGGAGTTCTTCCAAAACTCTGGGGTCCAATGGGACATGACAATGGTAGATGAGGCATACCGATGGATCGAAGAGACGGTCGTGTCTCAACCAGAGTTGGGCCCTTGGCAATCGGCTTTCAAAGAAGCGCTTGTGGAAGCTGGGGTTGGCCCTGATAATGGATATGATTTGAATCATGTTGTTGGGACAAGAATTGGAGGTTCTGTCTTTGATTCTAGAGGAAGGAGACATGGAGCTGTGGAGCTTCTCAACAAAGCTAATTCTAAAAATCTCAAAGTTGCAACCCAAGCCACAGTCAAAAGAATCATCTTCTCTCAATCTAATGGTGAGTATAGAAATACATCACTTTCATATATTTTTTATGTTTGAATATTTCATGAAAATGCTCTCTTTTTACTCTATCAATTTATAATTAGTTAGCATTTTTAGGGATAATAATCAAGTGTATAGCAACATTTTTAAAAAATTGTAAATATAGCAAAATCTATTAGTGATAGACTCCTACCAACAATATGGTCTATCATTATAGATTCCTGCCATTGATATGGTTTATTACTGATAGATCCCTACTAGTGATATGGTCTATCACTGATAGACTGGTCTATCACTAATAGACTCTTACTAATGATATTGTCTATCACGAATAGATTTGGAAAGCAAATTCTAAATTTGCTATATCTGCAAATTCCTTTACATTTTGCTATATTTTCAAATATTTTTGGCTTGATTGCTATATTTGAAACTGCCCCTAAATTTTATCACTATATATATATATATATATATATATATATATTCGTAAGTGTCCGAGTCAGCTTACGCGTACCTCGACTAATCTGACGGGACAACCTGTCCAACCCTACAATATTTGGATCACCAAATATTTAAATTTTATCAAATAATTCCTCATGAGTCATGAGTATAGTTGTTGTCTTTGAGACTTTTATTTTTATTTTTTTGACATGTGATCTTTTGACCTTGTCTTAAATTCCTCACCTAAAATCAATCTACAAGTTAAATCATACCAATTACAACTCACACATTCATATCAACCACAACATTTTTCTTCATAATCAATTATTTCTTTGTATCCTCTAGGTTTAACAGCAACTGGGGTCTTATATTCCGATTCAAAAGGCAAACTTCACACAGCAACCATCTCTAAAAATGGAGAAATCATTCTAAGTGCAGGCGCTATTGGAAGCCCTCAACTTCTTCTCTCAAGTGGGGTCGGCCCAAAATCTCATCTTTCATCTTTAAAACTACCTGTCGTTCTTCATAATCGACATGTTGGTCAATCCATGGCCGACAACCCCCGCTTCGGCGCTGCCATCGTCCTCCCATTTCTCACGCCGCCGACGTCGGTACAGATCGTCGGAACTTTAAAACCTAACATCCATATCGAATCTCTCTCAACTATTTTACCCTTTTCAATCTCCCCACCTTTTGCGCTTCTTCCACCTCGTTCCGCCGCCGTTAATCTAAGCCTCGCCATCTTCGCCGGAAAATTCTCTACCGTGTCCTCCACCGGAAGCCTACGGCTGGACGGCAGAAAAAACCCAATTGTCCGATTTAATTACTTATCACATCCGGATGATCTTGAACGGTGTGTTGAAGGGGTTAGAAAAGTTGGAGATTTGGTGAATACGAAAATTATGGAGAGGATTAAGACGAGGGATTTAGAGGGTAAAATGGGGTTTGAGTTTTTGGGGAGTTCGTTGCCGGAAAACATGTCGGATTATGGTTTGGTGGGAGAGTTCTGTCGGAAAACAGTGACGACGTTTTGGCATTATCATGGAGGATGTTTGGTCGGAAAAGTGGTCGACGGTAATTATAAACTCATCGGAATTAAAAACCTTCGTGTTGTCGATGGTTCTACTTTTTCTCTATCACCAGGAACAAATCCTATGGCCACCGTTATGATGTTGGGCAGGTGGGTCTCCTTTTTTACTACTAAGTTTTTTTTTATATATAATTTTTTGCCATGTCTTTTTACTAACAATAGAAAAGTTTTACAGACCATGATTAGATACAATCTAAGCTACATCTAATCTCATGCATTTCTTTACTATGTAATAAAAAATAATAATAAATAATACAATTTTTTTAAAAAAATATTTGACAGATGACAAAATTTTATTGGACAACTATACAAAAATAGGTAGAGCCAGCACAAAGTTTTTTTTGGAAAATTAACTTTTTAGTCTCCAAGTTTTAAATAATATGTGTGTTTGGTCCCTGAGTTTTAAAAATGTACATTTTTAGTCCCCGAGTTTATAAGAATAGGTCAATTTGGTCACTGAATTTTCAAAATATACTTTTTCAATCCCTGAATTTTTAAAAATAGGTTTAAAAGGTCCTTCAAATATTTTTTTTTATTTTTTTAAAAATAATTATACGATATTTAAAATTAGAAAAGAAATAATTTTAGAGAGATGATTTTTTAAAATTATTTTCTAATTTGAAATGTAATATAATTATCTAAAATAATAATAAAAAGGGAATTTTTCACAAATAGAAAAAAATGTCAAATTATTTACATAAAATAGCAAAAAAAAAATACTAATAGACATTGATAGACTTCTATCAGCATCTATCAGTGATAGAGTTCTATTATTTCTATCGCTGATAGATCCTGATAAGCTTCTATCAGCGTCTATCACAACTATCTAAAAATTTTGCTATTTTGTGTAAATAGTTTCTCTTATTTTTCTAATTTTAAAAAGTTCCCTAATAAAAAATTATTTGAGGGAACTCTTAAATCTGTTTTTAAAAATTCGAGACTAAAAAGATACATTTTGAAAATATAGGAATCAAATGAACCTATTCTTATCAACTTGGGGACTAAAAAGTTACATTTCTAAAACTTAGAGACTAAACGCACATATTCTAAAACTTTAGGACTAAAAATGTAATTTTTTTTTTTAATTTTCATTTACCTTTTCTATTGATTTCGTTTGGATAATAATTTTTTCTCATTTTTTGAAGTAAGAATATTATAATATATGCTCCACTAGTCAAAGACATGATTACCTCAACTAGGTCTAAAAGACAAAAGTAAGAATCAAATACTTGATGGCTATTTTAATCATTAATGTGTATTGTCCTCCATGAAGAAATTTACTTGAATCTCCACCTAAAAAAAATTGAATAAATTTAAGGTTGTTTTTAAATATAGTAAAATGAATCAACTTATTTACAAATATAGCAAAATATCACTATTTTATCACTTGTCATTATTTATCAATAATAGACATTGATAGACATCTATATAATTCTAAGCTTTCAAAAATAAAATCGTACTAAAATGAATATTGATTATGGTAGTAATAATTTTTGGGTATTTGTGGCCATTAAAATATCGATTCAAATTTGATAGAATTGAAAGTTTTTATTTATTTATTTTTTTTTTTTTGAGAAAGGTATTAAAATCTTTTATTATAAGATAACGAAGAGTTTCTTTTTCGCTATTAATAGTTTCTAACTTTTAATTACTACCTATCTATATTTCTTATTATGTACTTGATATTATTATTTATTTGTTTTACATATTGATTATATTTTGAAAATGTTTTTGCAGGTATGTTGGCCTTAAGATGTTGGAACAAAGATTGGGTCGAAGATCCAAACCATTTGGCTACTGAGGATAAAACAGAACGATCCTTCTGACATTTATTTTTCAAAATGTAGTAGAATTTTTGTCATTTGTTGAATAATAATCAGGGGTATAATTATTGAATGATATTTCTATTTCTTTGAATAAATGTACAAG

mRNA sequence

ATGGCCGATGATGGTTACACAAAGTTTGTACAAAACGCCAATACTTTACCAACAACAAAAGAATACGACTATATAATAGTAGGAGGAGGGACGGCCGGTTGCCCATTAGCTGCAACATTATCATCAAATTTCTCTGTTCTTGTTCTTGAAAGAGGAAGTGACCCTAATGCCTTCCCCTTGGTCCTATCCCAAGAAGGCATGGGAAACACTCTCACCGACGATGACGATGGCAGCAACCCATTCCAGCGATTTGTTTCTGAAGACGGTGTCGAGAACATTAGAGGTCGAGTCCTCGGTGGAGGTAGCATGATTAACGTCGGGTTCTACTCGAGAGCTCAGCCGGAGTTCTTCCAAAACTCTGGGGTCCAATGGGACATGACAATGGTAGATGAGGCATACCGATGGATCGAAGAGACGGTCGTGTCTCAACCAGAGTTGGGCCCTTGGCAATCGGCTTTCAAAGAAGCGCTTGTGGAAGCTGGGGTTGGCCCTGATAATGGATATGATTTGAATCATGTTGTTGGGACAAGAATTGGAGGTTCTGTCTTTGATTCTAGAGGAAGGAGACATGGAGCTGTGGAGCTTCTCAACAAAGCTAATTCTAAAAATCTCAAAGTTGCAACCCAAGCCACAGTCAAAAGAATCATCTTCTCTCAATCTAATGGTTTAACAGCAACTGGGGTCTTATATTCCGATTCAAAAGGCAAACTTCACACAGCAACCATCTCTAAAAATGGAGAAATCATTCTAAGTGCAGGCGCTATTGGAAGCCCTCAACTTCTTCTCTCAAGTGGGGTCGGCCCAAAATCTCATCTTTCATCTTTAAAACTACCTGTCGTTCTTCATAATCGACATGTTGGTCAATCCATGGCCGACAACCCCCGCTTCGGCGCTGCCATCGTCCTCCCATTTCTCACGCCGCCGACGTCGGTACAGATCGTCGGAACTTTAAAACCTAACATCCATATCGAATCTCTCTCAACTATTTTACCCTTTTCAATCTCCCCACCTTTTGCGCTTCTTCCACCTCGTTCCGCCGCCGTTAATCTAAGCCTCGCCATCTTCGCCGGAAAATTCTCTACCGTGTCCTCCACCGGAAGCCTACGGCTGGACGGCAGAAAAAACCCAATTGTCCGATTTAATTACTTATCACATCCGGATGATCTTGAACGGTGTGTTGAAGGGGTTAGAAAAGTTGGAGATTTGGTGAATACGAAAATTATGGAGAGGATTAAGACGAGGGATTTAGAGGGTAAAATGGGGTTTGAGTTTTTGGGGAGTTCGTTGCCGGAAAACATGTCGGATTATGGTTTGGTGGGAGAGTTCTGTCGGAAAACAGTGACGACGTTTTGGCATTATCATGGAGGATGTTTGGTCGGAAAAGTGGTCGACGGTAATTATAAACTCATCGGAATTAAAAACCTTCGTGTTGTCGATGGTTCTACTTTTTCTCTATCACCAGGAACAAATCCTATGGCCACCGTTATGATGTTGGGCAGGTATGTTGGCCTTAAGATGTTGGAACAAAGATTGGGTCGAAGATCCAAACCATTTGGCTACTGAGGATAAAACAGAACGATCCTTCTGACATTTATTTTTCAAAATGTAGTAGAATTTTTGTCATTTGTTGAATAATAATCAGGGGTATAATTATTGAATGATATTTCTATTTCTTTGAATAAATGTACAAG

Coding sequence (CDS)

ATGGCCGATGATGGTTACACAAAGTTTGTACAAAACGCCAATACTTTACCAACAACAAAAGAATACGACTATATAATAGTAGGAGGAGGGACGGCCGGTTGCCCATTAGCTGCAACATTATCATCAAATTTCTCTGTTCTTGTTCTTGAAAGAGGAAGTGACCCTAATGCCTTCCCCTTGGTCCTATCCCAAGAAGGCATGGGAAACACTCTCACCGACGATGACGATGGCAGCAACCCATTCCAGCGATTTGTTTCTGAAGACGGTGTCGAGAACATTAGAGGTCGAGTCCTCGGTGGAGGTAGCATGATTAACGTCGGGTTCTACTCGAGAGCTCAGCCGGAGTTCTTCCAAAACTCTGGGGTCCAATGGGACATGACAATGGTAGATGAGGCATACCGATGGATCGAAGAGACGGTCGTGTCTCAACCAGAGTTGGGCCCTTGGCAATCGGCTTTCAAAGAAGCGCTTGTGGAAGCTGGGGTTGGCCCTGATAATGGATATGATTTGAATCATGTTGTTGGGACAAGAATTGGAGGTTCTGTCTTTGATTCTAGAGGAAGGAGACATGGAGCTGTGGAGCTTCTCAACAAAGCTAATTCTAAAAATCTCAAAGTTGCAACCCAAGCCACAGTCAAAAGAATCATCTTCTCTCAATCTAATGGTTTAACAGCAACTGGGGTCTTATATTCCGATTCAAAAGGCAAACTTCACACAGCAACCATCTCTAAAAATGGAGAAATCATTCTAAGTGCAGGCGCTATTGGAAGCCCTCAACTTCTTCTCTCAAGTGGGGTCGGCCCAAAATCTCATCTTTCATCTTTAAAACTACCTGTCGTTCTTCATAATCGACATGTTGGTCAATCCATGGCCGACAACCCCCGCTTCGGCGCTGCCATCGTCCTCCCATTTCTCACGCCGCCGACGTCGGTACAGATCGTCGGAACTTTAAAACCTAACATCCATATCGAATCTCTCTCAACTATTTTACCCTTTTCAATCTCCCCACCTTTTGCGCTTCTTCCACCTCGTTCCGCCGCCGTTAATCTAAGCCTCGCCATCTTCGCCGGAAAATTCTCTACCGTGTCCTCCACCGGAAGCCTACGGCTGGACGGCAGAAAAAACCCAATTGTCCGATTTAATTACTTATCACATCCGGATGATCTTGAACGGTGTGTTGAAGGGGTTAGAAAAGTTGGAGATTTGGTGAATACGAAAATTATGGAGAGGATTAAGACGAGGGATTTAGAGGGTAAAATGGGGTTTGAGTTTTTGGGGAGTTCGTTGCCGGAAAACATGTCGGATTATGGTTTGGTGGGAGAGTTCTGTCGGAAAACAGTGACGACGTTTTGGCATTATCATGGAGGATGTTTGGTCGGAAAAGTGGTCGACGGTAATTATAAACTCATCGGAATTAAAAACCTTCGTGTTGTCGATGGTTCTACTTTTTCTCTATCACCAGGAACAAATCCTATGGCCACCGTTATGATGTTGGGCAGGTATGTTGGCCTTAAGATGTTGGAACAAAGATTGGGTCGAAGATCCAAACCATTTGGCTACTGA

Protein sequence

MADDGYTKFVQNANTLPTTKEYDYIIVGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPLVLSQEGMGNTLTDDDDGSNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFQNSGVQWDMTMVDEAYRWIEETVVSQPELGPWQSAFKEALVEAGVGPDNGYDLNHVVGTRIGGSVFDSRGRRHGAVELLNKANSKNLKVATQATVKRIIFSQSNGLTATGVLYSDSKGKLHTATISKNGEIILSAGAIGSPQLLLSSGVGPKSHLSSLKLPVVLHNRHVGQSMADNPRFGAAIVLPFLTPPTSVQIVGTLKPNIHIESLSTILPFSISPPFALLPPRSAAVNLSLAIFAGKFSTVSSTGSLRLDGRKNPIVRFNYLSHPDDLERCVEGVRKVGDLVNTKIMERIKTRDLEGKMGFEFLGSSLPENMSDYGLVGEFCRKTVTTFWHYHGGCLVGKVVDGNYKLIGIKNLRVVDGSTFSLSPGTNPMATVMMLGRYVGLKMLEQRLGRRSKPFGY
Homology
BLAST of Lsi11G007460 vs. ExPASy Swiss-Prot
Match: P52706 ((R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 511.5 bits (1316), Expect = 1.1e-143
Identity = 260/513 (50.68%), Postives = 346/513 (67.45%), Query Frame = 0

Query: 3   DDGYTKFVQNANTLPTTKEYDYIIVGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPLVL 62
           D  Y +F  +A  L     YDY+IVGGGT+GCPLAATLS  + VLVLERGS P A+P VL
Sbjct: 35  DFSYLRFAYDATDLELEGSYDYVIVGGGTSGCPLAATLSEKYKVLVLERGSLPTAYPNVL 94

Query: 63  SQEGMGNTLTDDDDGSNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFQNSGV 122
           + +G    L  +DDG  P +RFVSEDG++N+RGRVLGG SMIN G Y+RA    +  SGV
Sbjct: 95  TADGFVYNLQQEDDGKTPVERFVSEDGIDNVRGRVLGGTSMINAGVYARANTSIYSASGV 154

Query: 123 QWDMTMVDEAYRWIEETVVSQPELGPWQSAFKEALVEAGVGPDNGYDLNHVVGTRIGGSV 182
            WDM +V++ Y W+E+T+V +P   PWQS    A +EAGV P++G+ L+H  GTRI GS 
Sbjct: 155 DWDMDLVNKTYEWVEDTIVFKPNYQPWQSVTGTAFLEAGVDPNHGFSLDHEAGTRITGST 214

Query: 183 FDSRGRRHGAVELLNKANSKNLKVATQATVKRIIFSQSNGLTATGVLYSDSKGKLHTATI 242
           FD++G RH A ELLNK NS NL+V   A+V++IIFS + GLTATGV+Y DS G  H A +
Sbjct: 215 FDNKGTRHAADELLNKGNSNNLRVGVHASVEKIIFSNAPGLTATGVIYRDSNGTPHRAFV 274

Query: 243 SKNGEIILSAGAIGSPQLLLSSGVGPKSHLSSLKLPVVLHNRHVGQSMADNPRFGAAIVL 302
              GE+I+SAG IG+PQLLL SGVGP+S+LSSL +PVVL + +VGQ + DNPR    I+ 
Sbjct: 275 RSKGEVIVSAGTIGTPQLLLLSGVGPESYLSSLNIPVVLSHPYVGQFLHDNPRNFINILP 334

Query: 303 PFLTPPTSVQIVGTLKPNIHIESLSTILPFSISPPFALLPPRSAAV-NLSLAIFAGKFST 362
           P    PT V ++G    N   +   + LPF+ +PPF+  P  S  + N + A FA K + 
Sbjct: 335 PNPIEPTIVTVLGI--SNDFYQCSFSSLPFT-TPPFSFFPSTSYPLPNSTFAHFASKVAG 394

Query: 363 VSSTGSLRLDGRKN----PIVRFNYLSHPDDLERCVEGVRKVGDLVNTKIMERIKTRDLE 422
             S GSL L    N    P V+FNY S+P DL  CV G++K+G+L++T  ++  K  DL 
Sbjct: 395 PLSYGSLTLKSSSNVRVSPNVKFNYYSNPTDLSHCVSGMKKIGELLSTDALKPYKVEDLP 454

Query: 423 GKMGFEFLGSSLPENMSDYGLVGEFCRKTVTTFWHYHGGCLVGKVVDGNYKLIGIKNLRV 482
           G  GF  LG  LP++ +D      FCR++V ++WHYHGGCLVGKV+DG++++ GI  LRV
Sbjct: 455 GIEGFNILGIPLPKDQTDDAAFETFCRESVASYWHYHGGCLVGKVLDGDFRVTGIDALRV 514

Query: 483 VDGSTFSLSPGTNPMATVMMLGRYVGLKMLEQR 511
           VDGSTF  +P ++P    +MLGRYVG+K+L++R
Sbjct: 515 VDGSTFPYTPASHPQGFYLMLGRYVGIKILQER 544

BLAST of Lsi11G007460 vs. ExPASy Swiss-Prot
Match: Q9SSM2 ((R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 SV=1)

HSP 1 Score: 510.8 bits (1314), Expect = 1.9e-143
Identity = 252/515 (48.93%), Postives = 350/515 (67.96%), Query Frame = 0

Query: 5   GYTKFVQNANTLPTTKEYDYIIVGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPLVLSQ 64
           G+ +F+ NA    +   YDYIIVGGGTAGCPLAATLS +F VL+LERG  P   P V+S 
Sbjct: 37  GFMRFISNATDFASEDYYDYIIVGGGTAGCPLAATLSQSFRVLLLERGGVPYNRPNVMSH 96

Query: 65  EGMGNTLTDDDDGSNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFQNSGVQW 124
           +G   TLTD ++  +P Q F+SE+GV N RGRVLGG S IN GFYSRA  +FF+NSG+ W
Sbjct: 97  DGFLTTLTDVNNFDSPAQSFISEEGVPNARGRVLGGSSAINAGFYSRADKQFFENSGLVW 156

Query: 125 DMTMVDEAYRWIEETVVSQPELGPWQSAFKEALVEAGVGPDNGYDLNHVVGTRIGGSVFD 184
           D++ V+++Y W+E  +V +P+L  WQ+A ++AL+E GV P NG+ L H VGT+IGGS FD
Sbjct: 157 DLSSVNQSYEWVERAIVFRPQLRTWQTAIRDALLEVGVHPFNGFTLEHKVGTKIGGSTFD 216

Query: 185 SRGRRHGAVELLNKANSKNLKVATQATVKRIIFSQS-----NGLTATGVLYSDSKGKLHT 244
             GRRH + +LL  A S N++VA  ATV+R++ + S     + ++A GV+Y D  G+ H 
Sbjct: 217 RTGRRHSSADLLRYARSSNIRVAVYATVERVLLASSPSVSGSNVSAIGVVYRDQLGRFHH 276

Query: 245 ATISKNGEIILSAGAIGSPQLLLSSGVGPKSHLSSLKLPVVLHNRHVGQSMADNPRFGAA 304
           A I   GE+ILSAGA+GSPQLL  SG+GP+S+LS+  +PV L   HVG  + DNPR G +
Sbjct: 277 ALIRDRGEVILSAGALGSPQLLFLSGIGPRSYLSTWGIPVALDQPHVGDFVYDNPRNGIS 336

Query: 305 IVLPFLTPPTSVQIVGTLKPNIHIESLSTILPFSISPPFALLPPRSAAVNLSLAIFAGKF 364
           IV P     + +Q+VG  +    +E+ S ++PF+       +   ++ + + +     K 
Sbjct: 337 IVPPVPMENSLIQVVGVTEDGAFLEAASNVIPFASPLHSVFIRAPASPLYVPVTTIMEKI 396

Query: 365 STVSSTGSLRL---DGRKNPIVRFNYLSHPDDLERCVEGVRKVGDLVNTKIMERIKTRDL 424
               S G LRL   D R NP+VRFNY S P DLERCV G RK+G+++ ++ M+    R+ 
Sbjct: 397 LGPVSIGLLRLASTDVRINPVVRFNYFSDPQDLERCVNGTRKIGEILRSRAMQDFMIREW 456

Query: 425 EGKMGFEFLGSSLPENMSDYGLVGEFCRKTVTTFWHYHGGCLVGKVVDGNYKLIGIKNLR 484
            G   F F+G+ LP + S+  ++ +FCR+TV+T WHYHGG +VGKVVD + K+IG+ +LR
Sbjct: 457 FGNRRFRFVGAPLPVDQSNDLVMADFCRRTVSTIWHYHGGAVVGKVVDSDLKVIGVNSLR 516

Query: 485 VVDGSTFSLSPGTNPMATVMMLGRYVGLKMLEQRL 512
           +VDGSTF++SPGTNP AT+MMLGRY+GLKML +R+
Sbjct: 517 LVDGSTFNISPGTNPQATLMMLGRYMGLKMLRERM 551

BLAST of Lsi11G007460 vs. ExPASy Swiss-Prot
Match: Q945K2 ((R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 1.7e-141
Identity = 258/513 (50.29%), Postives = 343/513 (66.86%), Query Frame = 0

Query: 3   DDGYTKFVQNANTLPTTKEYDYIIVGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPLVL 62
           D  Y  F  +A  L     YDY+IVGGGT+GCPLAATLS  + VLVLERGS P A+P VL
Sbjct: 35  DFSYLSFAYDATDLELEGSYDYVIVGGGTSGCPLAATLSEKYKVLVLERGSLPTAYPNVL 94

Query: 63  SQEGMGNTLTDDDDGSNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFQNSGV 122
           + +G    L  +DDG  P +RFVSEDG++N+RGRVLGG S+IN G Y+RA    +  SGV
Sbjct: 95  TADGFVYNLQQEDDGKTPVERFVSEDGIDNVRGRVLGGTSIINAGVYARANTSIYSASGV 154

Query: 123 QWDMTMVDEAYRWIEETVVSQPELGPWQSAFKEALVEAGVGPDNGYDLNHVVGTRIGGSV 182
            WDM +V++ Y W+E+T+V +P    WQS  K A +EAGV P++G+ L+H  GTRI GS 
Sbjct: 155 DWDMDLVNQTYEWVEDTIVYKPNSQSWQSVTKTAFLEAGVHPNHGFSLDHEEGTRITGST 214

Query: 183 FDSRGRRHGAVELLNKANSKNLKVATQATVKRIIFSQSNGLTATGVLYSDSKGKLHTATI 242
           FD++G RH A ELLNK NS NL+V   A+V++IIFS + GLTATGV+Y DS G  H A +
Sbjct: 215 FDNKGTRHAADELLNKGNSNNLRVGVHASVEKIIFSNAPGLTATGVIYRDSNGTPHQAFV 274

Query: 243 SKNGEIILSAGAIGSPQLLLSSGVGPKSHLSSLKLPVVLHNRHVGQSMADNPRFGAAIVL 302
              GE+I+SAG IG+PQLLL SGVGP+S+LSSL +PVVL + +VGQ + DNPR    I+ 
Sbjct: 275 RSKGEVIVSAGTIGTPQLLLLSGVGPESYLSSLNIPVVLSHPYVGQFLHDNPRNFINILP 334

Query: 303 PFLTPPTSVQIVGTLKPNIHIESLSTILPFSISPPFALLPPRSAAV-NLSLAIFAGKFST 362
           P    PT V ++G    N   +   + LPF+ +PPF   P  S  + N + A FA K + 
Sbjct: 335 PNPIEPTIVTVLGI--SNDFYQCSFSSLPFT-TPPFGFFPSASYPLPNSTFAHFASKVAG 394

Query: 363 VSSTGSLRLDGRKN----PIVRFNYLSHPDDLERCVEGVRKVGDLVNTKIMERIKTRDLE 422
             S GSL L    N    P V+FNY S+  DL  CV G++K+G+L++T  ++  K  DL 
Sbjct: 395 PLSYGSLTLKSSSNVRVSPNVKFNYYSNLTDLSHCVSGMKKIGELLSTDALKPYKVEDLP 454

Query: 423 GKMGFEFLGSSLPENMSDYGLVGEFCRKTVTTFWHYHGGCLVGKVVDGNYKLIGIKNLRV 482
           G  GF  LG  LP++ +D      FCR++V ++WHYHGGCLVGKV+DG++++ GI  LRV
Sbjct: 455 GVEGFNILGIPLPKDQTDDAAFETFCRESVASYWHYHGGCLVGKVLDGDFRVTGINALRV 514

Query: 483 VDGSTFSLSPGTNPMATVMMLGRYVGLKMLEQR 511
           VDGSTF  +P ++P    +MLGRYVG+K+L++R
Sbjct: 515 VDGSTFPYTPASHPQGFYLMLGRYVGIKILQER 544

BLAST of Lsi11G007460 vs. ExPASy Swiss-Prot
Match: O82784 ((R)-mandelonitrile lyase 4 OS=Prunus serotina OX=23207 GN=MDL4 PE=2 SV=1)

HSP 1 Score: 495.4 bits (1274), Expect = 8.1e-139
Identity = 258/514 (50.19%), Postives = 345/514 (67.12%), Query Frame = 0

Query: 3   DDGYTKFVQNANTLPTTKEYDYIIVGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPLVL 62
           D GY KFV NA  L     YDYIIVGGGT+GCPLAATLS+N+SVLVLERG+    +P  L
Sbjct: 36  DFGYLKFVYNAVDLELEGSYDYIIVGGGTSGCPLAATLSANYSVLVLERGTIATEYPNTL 95

Query: 63  SQEGMGNTLTDDDDGSNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFQNSGV 122
           + +G    L   DDG  P +RFVSEDG++N+R R+LGG ++IN G Y+RA   F+ NSGV
Sbjct: 96  TVDGFAYNLQQQDDGKTPVERFVSEDGIDNVRSRILGGTTIINAGVYARANESFYNNSGV 155

Query: 123 QWDMTMVDEAYRWIEETVVSQPELGPWQSAFKEALVEAGVGPDNGYDLNHVVGTRIGGSV 182
           +WD+ +V+EAY W+E+ +V +P    WQS    A +EAGV PDNG+ L H  GTR+ GS 
Sbjct: 156 EWDLDLVNEAYEWVEDAIVYKPSNQSWQSITGTAFLEAGVHPDNGFGLVHEEGTRLTGST 215

Query: 183 FDSRGRRHGAVELLNKANSKNLKVATQATVKRIIFS-QSNGLTATGVLYSDSKGKLHTAT 242
           FD+ G RH + ELLNK +  NLKVA +A V++IIFS +S+GLTA GV+Y+DS G  H A 
Sbjct: 216 FDNSGTRHASDELLNKGDPDNLKVAVEAAVQKIIFSTESSGLTAVGVVYTDSNGTSHRAL 275

Query: 243 ISKNGEIILSAGAIGSPQLLLSSGVGPKSHLSSLKLPVVLHNRHVGQSMADNPRFGAAIV 302
           +S  GE+ILSAG +G+PQLLL SGVGP+S+L+SL + VV  + +VGQ + DNPR    I+
Sbjct: 276 VSGKGEVILSAGTLGTPQLLLLSGVGPESYLTSLNISVVASHPYVGQYVNDNPRNFINIL 335

Query: 303 LPFLTPPTSVQIVGTLKPNIHIESLSTILPFSISPPFALLPPRSAAV-NLSLAIFAGKFS 362
            P    P++V ++G +  + +  SLS+ LPF  +PPF+L P  S  + N + A    K  
Sbjct: 336 PPNPIEPSTVTVLG-ITSDFYQCSLSS-LPFD-TPPFSLFPTTSYPLPNQTFAHIVSKVP 395

Query: 363 TVSSTGSLRLDGRKN----PIVRFNYLSHPDDLERCVEGVRKVGDLVNTKIMERIKTRDL 422
              S GSL L    N    P V+FNY S P DL  CV G++K+G  ++T  ++  K  DL
Sbjct: 396 GPLSAGSLTLQSSSNVSVAPNVKFNYCSDPVDLTHCVSGMKKIGVFLSTDALKPYKVDDL 455

Query: 423 EGKMGFEFLGSSLPENMSDYGLVGEFCRKTVTTFWHYHGGCLVGKVVDGNYKLIGIKNLR 482
            G  GF  LG+ LPEN +D     +FCR TV ++WHYHGG +VGKV+DGN+++ GI  LR
Sbjct: 456 PGIDGFNILGTPLPENQTDDAAFEKFCRDTVASYWHYHGGAIVGKVIDGNFRVTGINALR 515

Query: 483 VVDGSTFSLSPGTNPMATVMMLGRYVGLKMLEQR 511
           VVDGSTF  +P ++P    +MLGRYVG K++++R
Sbjct: 516 VVDGSTFPATPASHPQGFYLMLGRYVGTKIVQER 546

BLAST of Lsi11G007460 vs. ExPASy Swiss-Prot
Match: P52707 ((R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1)

HSP 1 Score: 481.1 bits (1237), Expect = 1.6e-134
Identity = 248/514 (48.25%), Postives = 344/514 (66.93%), Query Frame = 0

Query: 3   DDGYTKFVQNANTLPTTKEYDYIIVGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPLVL 62
           D  Y  FV +A        YDYIIVGGGTAGCPLAATLS+N+SVLVLERGS P  +P +L
Sbjct: 35  DFSYLSFVYDATDPELEGSYDYIIVGGGTAGCPLAATLSANYSVLVLERGSLPTEYPNLL 94

Query: 63  SQEGMGNTLTDDDDGSNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFQNSGV 122
             +G    L  +DDG  P +RFVSEDG++N+RGRVLGG SMIN G Y RA   FF  +G+
Sbjct: 95  ISDGFVYNLQQEDDGKTPVERFVSEDGIDNVRGRVLGGTSMINAGVYVRANTSFFNQTGI 154

Query: 123 QWDMTMVDEAYRWIEETVVSQPELGPWQSAFKEALVEAGVGPDNGYDLNHVVGTRIGGSV 182
           +WDM +V++ Y W+E+T+V +P+   WQ+    A +EAG+ P+NG+ ++H+ GTR+ GS 
Sbjct: 155 EWDMDLVNQTYEWVEDTIVFEPDSQTWQTVIGTAYLEAGILPNNGFSVDHLAGTRLTGST 214

Query: 183 FDSRGRRHGAVELLNKANSKNLKVATQATVKRIIF-SQSNGLTATGVLYSDSKGKLHTAT 242
           FD+ G RH + ELLNK +  NL+VA QA V++IIF S ++G+TA GV+Y+DS G  H A 
Sbjct: 215 FDNNGTRHASDELLNKGDPNNLRVAVQAAVEKIIFSSNTSGVTAIGVIYTDSNGTTHQAF 274

Query: 243 ISKNGEIILSAGAIGSPQLLLSSGVGPKSHLSSLKLPVVLHNRHVGQSMADNPRFGAAIV 302
           +   GE+ILSAG IGSPQLLL SGVGP+S+L+SL + VV  + +VGQ + DNPR    I+
Sbjct: 275 VRGEGEVILSAGPIGSPQLLLLSGVGPESYLTSLNISVVASHPYVGQYIYDNPRNFINIL 334

Query: 303 LPFLTPPTSVQIVGTLKPNIHIESLSTILPFSISPPFALLPPRSAAV-NLSLAIFAGKFS 362
            P     ++V ++G +  + +  S+S+ LPF  +PPF+  P  S  + N + A    K  
Sbjct: 335 PPNPIEASTVTVLG-ITSDFYQCSISS-LPFD-TPPFSFFPTTSYPLPNQTFAHIVNKVP 394

Query: 363 TVSSTGSLRL----DGRKNPIVRFNYLSHPDDLERCVEGVRKVGDLVNTKIMERIKTRDL 422
              S G++ L    D R  P V+FNY S+  DL  CV G++K+G++++T  +E  K  DL
Sbjct: 395 GPLSHGTVTLNSSSDVRVGPNVKFNYYSNLTDLSHCVSGMKKLGEVLSTDALEPYKVEDL 454

Query: 423 EGKMGFEFLGSSLPENMSDYGLVGEFCRKTVTTFWHYHGGCLVGKVVDGNYKLIGIKNLR 482
            G  GF  LG  LPEN +D      FCR++V ++WHYHGGCLVGKV+D  +++ GI  LR
Sbjct: 455 PGIDGFNILGIPLPENQTDDAAFETFCRESVASYWHYHGGCLVGKVLDDGFRVTGINALR 514

Query: 483 VVDGSTFSLSPGTNPMATVMMLGRYVGLKMLEQR 511
           VVDGSTF  +P ++P    +MLGRY+G+++L++R
Sbjct: 515 VVDGSTFPSTPASHPQGFYLMLGRYMGIQILQER 545

BLAST of Lsi11G007460 vs. ExPASy TrEMBL
Match: A0A5A7UKP1 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold231G001010 PE=3 SV=1)

HSP 1 Score: 969.9 bits (2506), Expect = 4.2e-279
Identity = 477/517 (92.26%), Postives = 498/517 (96.32%), Query Frame = 0

Query: 3   DDGYTKFVQNANTLPTTKEYDYIIVGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPLVL 62
           DD Y KF+QNANTLPTT++YDYII+GGGTAGCPLAATLSSNFSVLVLERGSDPNAFP+VL
Sbjct: 31  DDSYIKFIQNANTLPTTEKYDYIIIGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPMVL 90

Query: 63  SQEGMGNTLTDDDDGSNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFQNSGV 122
           SQEGM NTLTDDD+G NPFQRF+SEDGVENIRGRVLGGGSMINVGFYSRAQPEFF+NS V
Sbjct: 91  SQEGMANTLTDDDNGHNPFQRFISEDGVENIRGRVLGGGSMINVGFYSRAQPEFFKNSSV 150

Query: 123 QWDMTMVDEAYRWIEETVVSQPELGPWQSAFKEALVEAGVGPDNGYDLNHVVGTRIGGSV 182
           QWDM MV+EAYRWIEETVVS+PELGPWQ AFKEALVEAGVGPDNGYDLNHVVGTRIGGS+
Sbjct: 151 QWDMAMVEEAYRWIEETVVSRPELGPWQLAFKEALVEAGVGPDNGYDLNHVVGTRIGGSI 210

Query: 183 FDSRGRRHGAVELLNKANSKNLKVATQATVKRIIFSQSNGLTATGVLYSDSKGKLHTATI 242
           FDSRG+RHGAVELLNKAN  NLKVATQATVKRIIFSQSNGL+ATGVLYSDS GKLH ATI
Sbjct: 211 FDSRGKRHGAVELLNKANPINLKVATQATVKRIIFSQSNGLSATGVLYSDSNGKLHKATI 270

Query: 243 SKNGEIILSAGAIGSPQLLLSSGVGPKSHLSSLKLPVVLHNRHVGQSMADNPRFGAAIVL 302
           SKNGEIILSAGAIGSPQLLLSSGVGPKSHLSSLKLP+VLHNRHVGQSMADNPRFGA +VL
Sbjct: 271 SKNGEIILSAGAIGSPQLLLSSGVGPKSHLSSLKLPLVLHNRHVGQSMADNPRFGATVVL 330

Query: 303 PFLTPPTSVQIVGTLKPNIHIESLSTILPFSISPPFALLPPRSAAVNLSLAIFAGKFSTV 362
           PFLT PTSVQ+VGTLKPNIHIESLSTILPFSISPPFALLPPRS+ VNLSLAIFAGKFSTV
Sbjct: 331 PFLTRPTSVQVVGTLKPNIHIESLSTILPFSISPPFALLPPRSSGVNLSLAIFAGKFSTV 390

Query: 363 SSTGSLRLDGRKNPIVRFNYLSHPDDLERCVEGVRKVGDLVNTKIMERIKTRDLEGKMGF 422
           SSTGSLRLD RKNPIVRFNYLSHPDDLERCVEGVRKVG+LVNTK+MERIKTRDLEGKMGF
Sbjct: 391 SSTGSLRLDRRKNPIVRFNYLSHPDDLERCVEGVRKVGELVNTKVMERIKTRDLEGKMGF 450

Query: 423 EFLGSSLPENMSDYGLVGEFCRKTVTTFWHYHGGCLVGKVVDGNYKLIGIKNLRVVDGST 482
           EFLG SLPENMSDYGLVGEFCRKTVTTFWHYHGGCLVGKVVDGNYK+IGI NLRVVDGST
Sbjct: 451 EFLGRSLPENMSDYGLVGEFCRKTVTTFWHYHGGCLVGKVVDGNYKVIGINNLRVVDGST 510

Query: 483 FSLSPGTNPMATVMMLGRYVGLKMLEQRLGRRSKPFG 520
           FSLSPGTNPMA VMMLGRYVGLKML QRLG+RSKPFG
Sbjct: 511 FSLSPGTNPMAAVMMLGRYVGLKMLHQRLGQRSKPFG 547

BLAST of Lsi11G007460 vs. ExPASy TrEMBL
Match: A0A1S3BTC3 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493044 PE=3 SV=1)

HSP 1 Score: 969.9 bits (2506), Expect = 4.2e-279
Identity = 477/517 (92.26%), Postives = 498/517 (96.32%), Query Frame = 0

Query: 3   DDGYTKFVQNANTLPTTKEYDYIIVGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPLVL 62
           DD Y KF+QNANTLPTT++YDYII+GGGTAGCPLAATLSSNFSVLVLERGSDPNAFP+VL
Sbjct: 31  DDSYIKFIQNANTLPTTEKYDYIIIGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPMVL 90

Query: 63  SQEGMGNTLTDDDDGSNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFQNSGV 122
           SQEGM NTLTDDD+G NPFQRF+SEDGVENIRGRVLGGGSMINVGFYSRAQPEFF+NS V
Sbjct: 91  SQEGMANTLTDDDNGHNPFQRFISEDGVENIRGRVLGGGSMINVGFYSRAQPEFFKNSSV 150

Query: 123 QWDMTMVDEAYRWIEETVVSQPELGPWQSAFKEALVEAGVGPDNGYDLNHVVGTRIGGSV 182
           QWDM MV+EAYRWIEETVVS+PELGPWQ AFKEALVEAGVGPDNGYDLNHVVGTRIGGS+
Sbjct: 151 QWDMAMVEEAYRWIEETVVSRPELGPWQLAFKEALVEAGVGPDNGYDLNHVVGTRIGGSI 210

Query: 183 FDSRGRRHGAVELLNKANSKNLKVATQATVKRIIFSQSNGLTATGVLYSDSKGKLHTATI 242
           FDSRG+RHGAVELLNKAN  NLKVATQATVKRIIFSQSNGL+ATGVLYSDS GKLH ATI
Sbjct: 211 FDSRGKRHGAVELLNKANPINLKVATQATVKRIIFSQSNGLSATGVLYSDSNGKLHKATI 270

Query: 243 SKNGEIILSAGAIGSPQLLLSSGVGPKSHLSSLKLPVVLHNRHVGQSMADNPRFGAAIVL 302
           SKNGEIILSAGAIGSPQLLLSSGVGPKSHLSSLKLP+VLHNRHVGQSMADNPRFGA +VL
Sbjct: 271 SKNGEIILSAGAIGSPQLLLSSGVGPKSHLSSLKLPLVLHNRHVGQSMADNPRFGATVVL 330

Query: 303 PFLTPPTSVQIVGTLKPNIHIESLSTILPFSISPPFALLPPRSAAVNLSLAIFAGKFSTV 362
           PFLT PTSVQ+VGTLKPNIHIESLSTILPFSISPPFALLPPRS+ VNLSLAIFAGKFSTV
Sbjct: 331 PFLTRPTSVQVVGTLKPNIHIESLSTILPFSISPPFALLPPRSSGVNLSLAIFAGKFSTV 390

Query: 363 SSTGSLRLDGRKNPIVRFNYLSHPDDLERCVEGVRKVGDLVNTKIMERIKTRDLEGKMGF 422
           SSTGSLRLD RKNPIVRFNYLSHPDDLERCVEGVRKVG+LVNTK+MERIKTRDLEGKMGF
Sbjct: 391 SSTGSLRLDRRKNPIVRFNYLSHPDDLERCVEGVRKVGELVNTKVMERIKTRDLEGKMGF 450

Query: 423 EFLGSSLPENMSDYGLVGEFCRKTVTTFWHYHGGCLVGKVVDGNYKLIGIKNLRVVDGST 482
           EFLG SLPENMSDYGLVGEFCRKTVTTFWHYHGGCLVGKVVDGNYK+IGI NLRVVDGST
Sbjct: 451 EFLGRSLPENMSDYGLVGEFCRKTVTTFWHYHGGCLVGKVVDGNYKVIGINNLRVVDGST 510

Query: 483 FSLSPGTNPMATVMMLGRYVGLKMLEQRLGRRSKPFG 520
           FSLSPGTNPMA VMMLGRYVGLKML QRLG+RSKPFG
Sbjct: 511 FSLSPGTNPMAAVMMLGRYVGLKMLHQRLGQRSKPFG 547

BLAST of Lsi11G007460 vs. ExPASy TrEMBL
Match: A0A0A0LMC2 (GMC_OxRdtase_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G057070 PE=3 SV=1)

HSP 1 Score: 967.2 bits (2499), Expect = 2.7e-278
Identity = 476/517 (92.07%), Postives = 501/517 (96.91%), Query Frame = 0

Query: 3   DDGYTKFVQNANTLPTTKEYDYIIVGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPLVL 62
           DD Y KF+QNANTLPTT++YDYII+GGGTAGCPLAATLSSNFSVLVLERGSDPNAFP+VL
Sbjct: 30  DDSYIKFIQNANTLPTTEKYDYIIIGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPMVL 89

Query: 63  SQEGMGNTLTDDDDGSNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFQNSGV 122
           SQEGM NTLT+DDDG NPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFF+NS V
Sbjct: 90  SQEGMANTLTEDDDGHNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFKNSSV 149

Query: 123 QWDMTMVDEAYRWIEETVVSQPELGPWQSAFKEALVEAGVGPDNGYDLNHVVGTRIGGSV 182
           QW+M MV+EAYRWIEETVVS+PELGPWQ AFKEALVEAGVGPDNGYDL+HVVGTRIGGS+
Sbjct: 150 QWNMEMVEEAYRWIEETVVSRPELGPWQLAFKEALVEAGVGPDNGYDLDHVVGTRIGGSI 209

Query: 183 FDSRGRRHGAVELLNKANSKNLKVATQATVKRIIFSQSNGLTATGVLYSDSKGKLHTATI 242
           FDSRG+RHGAVELLNKAN  NLKVATQATVKRIIFS+SNGL+ATGVLYSDS GKLH ATI
Sbjct: 210 FDSRGKRHGAVELLNKANPINLKVATQATVKRIIFSRSNGLSATGVLYSDSNGKLHKATI 269

Query: 243 SKNGEIILSAGAIGSPQLLLSSGVGPKSHLSSLKLPVVLHNRHVGQSMADNPRFGAAIVL 302
           S+NGEIILSAGAIGSPQLLLSSGVGPKS LSSLKLP+VLHNRHVGQSMADNPR GAAIVL
Sbjct: 270 SRNGEIILSAGAIGSPQLLLSSGVGPKSDLSSLKLPLVLHNRHVGQSMADNPRLGAAIVL 329

Query: 303 PFLTPPTSVQIVGTLKPNIHIESLSTILPFSISPPFALLPPRSAAVNLSLAIFAGKFSTV 362
           PFLTPPTSVQ+VGTLKPNIHIESLSTILPFSISPPFALLPPRS+AVNLSLAIFAGKFSTV
Sbjct: 330 PFLTPPTSVQVVGTLKPNIHIESLSTILPFSISPPFALLPPRSSAVNLSLAIFAGKFSTV 389

Query: 363 SSTGSLRLDGRKNPIVRFNYLSHPDDLERCVEGVRKVGDLVNTKIMERIKTRDLEGKMGF 422
           SSTGSLRLD RKNPIVRFNYLSHPDDLERCVEGVRKVG+LVNTK+MERIKTRDLEGKMGF
Sbjct: 390 SSTGSLRLDRRKNPIVRFNYLSHPDDLERCVEGVRKVGELVNTKVMERIKTRDLEGKMGF 449

Query: 423 EFLGSSLPENMSDYGLVGEFCRKTVTTFWHYHGGCLVGKVVDGNYKLIGIKNLRVVDGST 482
           EFLGSSLPENMSDYGLVGEFCRKTVTTFWHYHGGC+VGKVVDGNYK+IG+ NLRVVDGST
Sbjct: 450 EFLGSSLPENMSDYGLVGEFCRKTVTTFWHYHGGCVVGKVVDGNYKVIGVNNLRVVDGST 509

Query: 483 FSLSPGTNPMATVMMLGRYVGLKMLEQRLGRRSKPFG 520
           FSLSPGTNPMATVMMLGRYVGLKML QRLG+RSKPFG
Sbjct: 510 FSLSPGTNPMATVMMLGRYVGLKMLHQRLGQRSKPFG 546

BLAST of Lsi11G007460 vs. ExPASy TrEMBL
Match: A0A6J1EN54 ((R)-mandelonitrile lyase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111436125 PE=3 SV=1)

HSP 1 Score: 914.8 bits (2363), Expect = 1.6e-262
Identity = 450/510 (88.24%), Postives = 480/510 (94.12%), Query Frame = 0

Query: 3   DDGYTKFVQNANTLPTTKEYDYIIVGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPLVL 62
           DD Y KFVQ ANTLPT +EYDYII+GGGTAGCPLAATLSSNFSVLVLERGSDPNAFP VL
Sbjct: 30  DDSYMKFVQKANTLPTREEYDYIIIGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPSVL 89

Query: 63  SQEGMGNTLTDDDDGSNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFQNSGV 122
           SQ+G+ NTLTD+DDG NPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFF++SG+
Sbjct: 90  SQQGLANTLTDNDDGLNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFKSSGI 149

Query: 123 QWDMTMVDEAYRWIEETVVSQPELGPWQSAFKEALVEAGVGPDNGYDLNHVVGTRIGGSV 182
           QWDMT V++AY+WIEETVVS+PEL PWQSAF+E L+EAGVGPDNGYDL H VGTR GGS+
Sbjct: 150 QWDMTAVEKAYQWIEETVVSRPELSPWQSAFREVLLEAGVGPDNGYDLKHGVGTRTGGSI 209

Query: 183 FDSRGRRHGAVELLNKANSKNLKVATQATVKRIIFSQSNGLTATGVLYSDSKGKLHTATI 242
           FDSRGRRHGAVELLNKA+ +NL+VATQATVKRIIFSQSNGL+A+GVLYSD KGKLH ATI
Sbjct: 210 FDSRGRRHGAVELLNKADPRNLRVATQATVKRIIFSQSNGLSASGVLYSDFKGKLHKATI 269

Query: 243 SKNGEIILSAGAIGSPQLLLSSGVGPKSHLSSLKLPVVLHNRHVGQSMADNPRFGAAIVL 302
           SKNGEIIL+AGAIGSP LLL SGVGPKSHLSSLKLPVVLHNRHVGQSMADNPRFGAAIVL
Sbjct: 270 SKNGEIILTAGAIGSPHLLLQSGVGPKSHLSSLKLPVVLHNRHVGQSMADNPRFGAAIVL 329

Query: 303 PFLTPPTSVQIVGTLKPNIHIESLSTILPFSISPPFALLPPRSAAVNLSLAIFAGKFSTV 362
           PFLTPPTSVQ+VGTLK NIHIESLS+ILPFSI PPF LLPPRS AVNLSLA+FAGKFSTV
Sbjct: 330 PFLTPPTSVQVVGTLKRNIHIESLSSILPFSIFPPFGLLPPRSTAVNLSLAVFAGKFSTV 389

Query: 363 SSTGSLRLDGRKNPIVRFNYLSHPDDLERCVEGVRKVGDLVNTKIMERIKTRDLEGKMGF 422
           SS GSLRLDGRKNPIVRFNYLSHPDD+ERCVEGVRKVGDLVNTK MERIKT DLEGK GF
Sbjct: 390 SSVGSLRLDGRKNPIVRFNYLSHPDDVERCVEGVRKVGDLVNTKSMERIKTGDLEGKKGF 449

Query: 423 EFLGSSLPENMSDYGLVGEFCRKTVTTFWHYHGGCLVGKVVDGNYKLIGIKNLRVVDGST 482
           +FLGS LPENMSDY LVG+FCRKTVTTFWHYHGGCLVGKVVD NY++IGIK LRVVDGST
Sbjct: 450 KFLGSPLPENMSDYKLVGDFCRKTVTTFWHYHGGCLVGKVVDDNYRVIGIKKLRVVDGST 509

Query: 483 FSLSPGTNPMATVMMLGRYVGLKMLEQRLG 513
           FSLSPGTNPMATVMMLGRYVGLKML+QRLG
Sbjct: 510 FSLSPGTNPMATVMMLGRYVGLKMLQQRLG 539

BLAST of Lsi11G007460 vs. ExPASy TrEMBL
Match: A0A6J1K9L8 ((R)-mandelonitrile lyase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111492348 PE=3 SV=1)

HSP 1 Score: 913.7 bits (2360), Expect = 3.5e-262
Identity = 448/510 (87.84%), Postives = 481/510 (94.31%), Query Frame = 0

Query: 3   DDGYTKFVQNANTLPTTKEYDYIIVGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPLVL 62
           DD Y KFVQNANTLPT +EYDYII+GGGTAGCPLAATLSSNFSVLVLERGSDPNAFP VL
Sbjct: 30  DDSYMKFVQNANTLPTREEYDYIIIGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPSVL 89

Query: 63  SQEGMGNTLTDDDDGSNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFQNSGV 122
           SQ+G+ NTLTD+DDG NPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFQ+SGV
Sbjct: 90  SQQGLANTLTDNDDGLNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFQSSGV 149

Query: 123 QWDMTMVDEAYRWIEETVVSQPELGPWQSAFKEALVEAGVGPDNGYDLNHVVGTRIGGSV 182
           QWDM  V++AY+WIEETVVS+PEL PWQSAF+EAL+EAGVG DNGYDLNHVVGTR GGS+
Sbjct: 150 QWDMMAVEKAYQWIEETVVSRPELSPWQSAFREALLEAGVGRDNGYDLNHVVGTRTGGSI 209

Query: 183 FDSRGRRHGAVELLNKANSKNLKVATQATVKRIIFSQSNGLTATGVLYSDSKGKLHTATI 242
           FDS GRRHGAVELLNKA+ +NL+VATQATVKRIIFSQSNGL+A+GVLYSD KGKLH ATI
Sbjct: 210 FDSSGRRHGAVELLNKADPRNLRVATQATVKRIIFSQSNGLSASGVLYSDFKGKLHKATI 269

Query: 243 SKNGEIILSAGAIGSPQLLLSSGVGPKSHLSSLKLPVVLHNRHVGQSMADNPRFGAAIVL 302
           S+NGEIIL+AGAIGSP LLL SGVGP+SHLSSLKLPVV HN+HVGQ MADNPRFGAAIVL
Sbjct: 270 SRNGEIILTAGAIGSPHLLLQSGVGPRSHLSSLKLPVVFHNQHVGQFMADNPRFGAAIVL 329

Query: 303 PFLTPPTSVQIVGTLKPNIHIESLSTILPFSISPPFALLPPRSAAVNLSLAIFAGKFSTV 362
           PFLTPPTSVQ+VGTLKPNIHIESLS+ILPFSISPPF LLPPRS AVNLSLA+FAGKFSTV
Sbjct: 330 PFLTPPTSVQVVGTLKPNIHIESLSSILPFSISPPFGLLPPRSTAVNLSLAVFAGKFSTV 389

Query: 363 SSTGSLRLDGRKNPIVRFNYLSHPDDLERCVEGVRKVGDLVNTKIMERIKTRDLEGKMGF 422
           SS GSLRLDGRKNPIVRFNYLSHPDD++RCVEGVRKVGDLVNTK MERIKT DL+GK GF
Sbjct: 390 SSVGSLRLDGRKNPIVRFNYLSHPDDVQRCVEGVRKVGDLVNTKSMERIKTGDLDGKKGF 449

Query: 423 EFLGSSLPENMSDYGLVGEFCRKTVTTFWHYHGGCLVGKVVDGNYKLIGIKNLRVVDGST 482
           EFLG+ LPENMSDY LVG+FCRKTVTTFWHYHGGCLVGKVVD NY++IGIK LRVVDGST
Sbjct: 450 EFLGAPLPENMSDYRLVGDFCRKTVTTFWHYHGGCLVGKVVDDNYRVIGIKKLRVVDGST 509

Query: 483 FSLSPGTNPMATVMMLGRYVGLKMLEQRLG 513
           FSLSPGTNPMATVMMLGRYVGLKML+QRLG
Sbjct: 510 FSLSPGTNPMATVMMLGRYVGLKMLQQRLG 539

BLAST of Lsi11G007460 vs. NCBI nr
Match: XP_038890837.1 ((R)-mandelonitrile lyase 1-like [Benincasa hispida])

HSP 1 Score: 971.5 bits (2510), Expect = 2.9e-279
Identity = 478/518 (92.28%), Postives = 504/518 (97.30%), Query Frame = 0

Query: 3   DDGYTKFVQNANTLPTTKEYDYIIVGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPLVL 62
           DD + KFVQNAN L TT++YDYII+GGGTAGCPLAATLSSNFSVLVLERGSDPNAFPLVL
Sbjct: 30  DDSFMKFVQNANALTTTEKYDYIIIGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPLVL 89

Query: 63  SQEGMGNTLTDDDDGSNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFQNSGV 122
           SQEGM NTLTDDDDGSNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFQNSGV
Sbjct: 90  SQEGMANTLTDDDDGSNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFQNSGV 149

Query: 123 QWDMTMVDEAYRWIEETVVSQPELGPWQSAFKEALVEAGVGPDNGYDLNHVVGTRIGGSV 182
           QWDM +V+EAYRWIE+T+VSQPELGPWQSAF+EALVEAGVGPDNGYDL+HVVGTRIGGS+
Sbjct: 150 QWDMKLVEEAYRWIEDTIVSQPELGPWQSAFREALVEAGVGPDNGYDLSHVVGTRIGGSI 209

Query: 183 FDSRGRRHGAVELLNKANSKNLKVATQATVKRIIFSQSNGLTATGVLYSDSKGKLHTATI 242
           FDSRGRRHGAVELLNKAN KNLKVATQATVKRIIFSQSNGLTATGVLYSDSKGK+H ATI
Sbjct: 210 FDSRGRRHGAVELLNKANPKNLKVATQATVKRIIFSQSNGLTATGVLYSDSKGKVHKATI 269

Query: 243 SKNGEIILSAGAIGSPQLLLSSGVGPKSHLSSLKLPVVLHNRHVGQSMADNPRFGAAIVL 302
           SKNGEIILSAGAIGSPQLLLSSG+GP+SHLSSLKL VVL N HVGQSMADNPRFGAAIVL
Sbjct: 270 SKNGEIILSAGAIGSPQLLLSSGIGPRSHLSSLKLHVVLDNPHVGQSMADNPRFGAAIVL 329

Query: 303 PFLTPPTSVQIVGTLKPNIHIESLSTILPFSISPPFALLPPRSAAVNLSLAIFAGKFSTV 362
           PFLTPPTSVQ+VGTLKPNIHIESLSTILPFSISPPFALLPPRS+AVNLS+AIFAGKFSTV
Sbjct: 330 PFLTPPTSVQVVGTLKPNIHIESLSTILPFSISPPFALLPPRSSAVNLSVAIFAGKFSTV 389

Query: 363 SSTGSLRLDGRKNPIVRFNYLSHPDDLERCVEGVRKVGDLVNTKIMERIKTRDLEGKMGF 422
           SSTGSLRLDG KNPIVRFNYLSHPDDLERCVEGVRKVG+LVNT++MERIKTRDLEGKMGF
Sbjct: 390 SSTGSLRLDGGKNPIVRFNYLSHPDDLERCVEGVRKVGELVNTQVMERIKTRDLEGKMGF 449

Query: 423 EFLGSSLPENMSDYGLVGEFCRKTVTTFWHYHGGCLVGKVVDGNYKLIGIKNLRVVDGST 482
           +FLG+SLPENMSDY LVGEFCRKTVTTFWHYHGGCLVGKVVDGNYK+IGIKNLRVVDGS 
Sbjct: 450 KFLGTSLPENMSDYCLVGEFCRKTVTTFWHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSI 509

Query: 483 FSLSPGTNPMATVMMLGRYVGLKMLEQRLGRRSKPFGY 521
           FSLSPGTNPMATVMMLGRYVGLKM+E+RLG+RSKPFGY
Sbjct: 510 FSLSPGTNPMATVMMLGRYVGLKMMEERLGQRSKPFGY 547

BLAST of Lsi11G007460 vs. NCBI nr
Match: XP_008451889.1 (PREDICTED: (R)-mandelonitrile lyase 1-like [Cucumis melo] >KAA0055628.1 (R)-mandelonitrile lyase 1-like [Cucumis melo var. makuwa] >TYK10918.1 (R)-mandelonitrile lyase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 969.9 bits (2506), Expect = 8.6e-279
Identity = 477/517 (92.26%), Postives = 498/517 (96.32%), Query Frame = 0

Query: 3   DDGYTKFVQNANTLPTTKEYDYIIVGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPLVL 62
           DD Y KF+QNANTLPTT++YDYII+GGGTAGCPLAATLSSNFSVLVLERGSDPNAFP+VL
Sbjct: 31  DDSYIKFIQNANTLPTTEKYDYIIIGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPMVL 90

Query: 63  SQEGMGNTLTDDDDGSNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFQNSGV 122
           SQEGM NTLTDDD+G NPFQRF+SEDGVENIRGRVLGGGSMINVGFYSRAQPEFF+NS V
Sbjct: 91  SQEGMANTLTDDDNGHNPFQRFISEDGVENIRGRVLGGGSMINVGFYSRAQPEFFKNSSV 150

Query: 123 QWDMTMVDEAYRWIEETVVSQPELGPWQSAFKEALVEAGVGPDNGYDLNHVVGTRIGGSV 182
           QWDM MV+EAYRWIEETVVS+PELGPWQ AFKEALVEAGVGPDNGYDLNHVVGTRIGGS+
Sbjct: 151 QWDMAMVEEAYRWIEETVVSRPELGPWQLAFKEALVEAGVGPDNGYDLNHVVGTRIGGSI 210

Query: 183 FDSRGRRHGAVELLNKANSKNLKVATQATVKRIIFSQSNGLTATGVLYSDSKGKLHTATI 242
           FDSRG+RHGAVELLNKAN  NLKVATQATVKRIIFSQSNGL+ATGVLYSDS GKLH ATI
Sbjct: 211 FDSRGKRHGAVELLNKANPINLKVATQATVKRIIFSQSNGLSATGVLYSDSNGKLHKATI 270

Query: 243 SKNGEIILSAGAIGSPQLLLSSGVGPKSHLSSLKLPVVLHNRHVGQSMADNPRFGAAIVL 302
           SKNGEIILSAGAIGSPQLLLSSGVGPKSHLSSLKLP+VLHNRHVGQSMADNPRFGA +VL
Sbjct: 271 SKNGEIILSAGAIGSPQLLLSSGVGPKSHLSSLKLPLVLHNRHVGQSMADNPRFGATVVL 330

Query: 303 PFLTPPTSVQIVGTLKPNIHIESLSTILPFSISPPFALLPPRSAAVNLSLAIFAGKFSTV 362
           PFLT PTSVQ+VGTLKPNIHIESLSTILPFSISPPFALLPPRS+ VNLSLAIFAGKFSTV
Sbjct: 331 PFLTRPTSVQVVGTLKPNIHIESLSTILPFSISPPFALLPPRSSGVNLSLAIFAGKFSTV 390

Query: 363 SSTGSLRLDGRKNPIVRFNYLSHPDDLERCVEGVRKVGDLVNTKIMERIKTRDLEGKMGF 422
           SSTGSLRLD RKNPIVRFNYLSHPDDLERCVEGVRKVG+LVNTK+MERIKTRDLEGKMGF
Sbjct: 391 SSTGSLRLDRRKNPIVRFNYLSHPDDLERCVEGVRKVGELVNTKVMERIKTRDLEGKMGF 450

Query: 423 EFLGSSLPENMSDYGLVGEFCRKTVTTFWHYHGGCLVGKVVDGNYKLIGIKNLRVVDGST 482
           EFLG SLPENMSDYGLVGEFCRKTVTTFWHYHGGCLVGKVVDGNYK+IGI NLRVVDGST
Sbjct: 451 EFLGRSLPENMSDYGLVGEFCRKTVTTFWHYHGGCLVGKVVDGNYKVIGINNLRVVDGST 510

Query: 483 FSLSPGTNPMATVMMLGRYVGLKMLEQRLGRRSKPFG 520
           FSLSPGTNPMA VMMLGRYVGLKML QRLG+RSKPFG
Sbjct: 511 FSLSPGTNPMAAVMMLGRYVGLKMLHQRLGQRSKPFG 547

BLAST of Lsi11G007460 vs. NCBI nr
Match: XP_011648924.1 ((R)-mandelonitrile lyase 1 [Cucumis sativus])

HSP 1 Score: 967.2 bits (2499), Expect = 5.6e-278
Identity = 476/517 (92.07%), Postives = 501/517 (96.91%), Query Frame = 0

Query: 3   DDGYTKFVQNANTLPTTKEYDYIIVGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPLVL 62
           DD Y KF+QNANTLPTT++YDYII+GGGTAGCPLAATLSSNFSVLVLERGSDPNAFP+VL
Sbjct: 30  DDSYIKFIQNANTLPTTEKYDYIIIGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPMVL 89

Query: 63  SQEGMGNTLTDDDDGSNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFQNSGV 122
           SQEGM NTLT+DDDG NPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFF+NS V
Sbjct: 90  SQEGMANTLTEDDDGHNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFKNSSV 149

Query: 123 QWDMTMVDEAYRWIEETVVSQPELGPWQSAFKEALVEAGVGPDNGYDLNHVVGTRIGGSV 182
           QW+M MV+EAYRWIEETVVS+PELGPWQ AFKEALVEAGVGPDNGYDL+HVVGTRIGGS+
Sbjct: 150 QWNMEMVEEAYRWIEETVVSRPELGPWQLAFKEALVEAGVGPDNGYDLDHVVGTRIGGSI 209

Query: 183 FDSRGRRHGAVELLNKANSKNLKVATQATVKRIIFSQSNGLTATGVLYSDSKGKLHTATI 242
           FDSRG+RHGAVELLNKAN  NLKVATQATVKRIIFS+SNGL+ATGVLYSDS GKLH ATI
Sbjct: 210 FDSRGKRHGAVELLNKANPINLKVATQATVKRIIFSRSNGLSATGVLYSDSNGKLHKATI 269

Query: 243 SKNGEIILSAGAIGSPQLLLSSGVGPKSHLSSLKLPVVLHNRHVGQSMADNPRFGAAIVL 302
           S+NGEIILSAGAIGSPQLLLSSGVGPKS LSSLKLP+VLHNRHVGQSMADNPR GAAIVL
Sbjct: 270 SRNGEIILSAGAIGSPQLLLSSGVGPKSDLSSLKLPLVLHNRHVGQSMADNPRLGAAIVL 329

Query: 303 PFLTPPTSVQIVGTLKPNIHIESLSTILPFSISPPFALLPPRSAAVNLSLAIFAGKFSTV 362
           PFLTPPTSVQ+VGTLKPNIHIESLSTILPFSISPPFALLPPRS+AVNLSLAIFAGKFSTV
Sbjct: 330 PFLTPPTSVQVVGTLKPNIHIESLSTILPFSISPPFALLPPRSSAVNLSLAIFAGKFSTV 389

Query: 363 SSTGSLRLDGRKNPIVRFNYLSHPDDLERCVEGVRKVGDLVNTKIMERIKTRDLEGKMGF 422
           SSTGSLRLD RKNPIVRFNYLSHPDDLERCVEGVRKVG+LVNTK+MERIKTRDLEGKMGF
Sbjct: 390 SSTGSLRLDRRKNPIVRFNYLSHPDDLERCVEGVRKVGELVNTKVMERIKTRDLEGKMGF 449

Query: 423 EFLGSSLPENMSDYGLVGEFCRKTVTTFWHYHGGCLVGKVVDGNYKLIGIKNLRVVDGST 482
           EFLGSSLPENMSDYGLVGEFCRKTVTTFWHYHGGC+VGKVVDGNYK+IG+ NLRVVDGST
Sbjct: 450 EFLGSSLPENMSDYGLVGEFCRKTVTTFWHYHGGCVVGKVVDGNYKVIGVNNLRVVDGST 509

Query: 483 FSLSPGTNPMATVMMLGRYVGLKMLEQRLGRRSKPFG 520
           FSLSPGTNPMATVMMLGRYVGLKML QRLG+RSKPFG
Sbjct: 510 FSLSPGTNPMATVMMLGRYVGLKMLHQRLGQRSKPFG 546

BLAST of Lsi11G007460 vs. NCBI nr
Match: XP_022929582.1 ((R)-mandelonitrile lyase 1-like [Cucurbita moschata])

HSP 1 Score: 914.8 bits (2363), Expect = 3.3e-262
Identity = 450/510 (88.24%), Postives = 480/510 (94.12%), Query Frame = 0

Query: 3   DDGYTKFVQNANTLPTTKEYDYIIVGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPLVL 62
           DD Y KFVQ ANTLPT +EYDYII+GGGTAGCPLAATLSSNFSVLVLERGSDPNAFP VL
Sbjct: 30  DDSYMKFVQKANTLPTREEYDYIIIGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPSVL 89

Query: 63  SQEGMGNTLTDDDDGSNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFQNSGV 122
           SQ+G+ NTLTD+DDG NPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFF++SG+
Sbjct: 90  SQQGLANTLTDNDDGLNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFKSSGI 149

Query: 123 QWDMTMVDEAYRWIEETVVSQPELGPWQSAFKEALVEAGVGPDNGYDLNHVVGTRIGGSV 182
           QWDMT V++AY+WIEETVVS+PEL PWQSAF+E L+EAGVGPDNGYDL H VGTR GGS+
Sbjct: 150 QWDMTAVEKAYQWIEETVVSRPELSPWQSAFREVLLEAGVGPDNGYDLKHGVGTRTGGSI 209

Query: 183 FDSRGRRHGAVELLNKANSKNLKVATQATVKRIIFSQSNGLTATGVLYSDSKGKLHTATI 242
           FDSRGRRHGAVELLNKA+ +NL+VATQATVKRIIFSQSNGL+A+GVLYSD KGKLH ATI
Sbjct: 210 FDSRGRRHGAVELLNKADPRNLRVATQATVKRIIFSQSNGLSASGVLYSDFKGKLHKATI 269

Query: 243 SKNGEIILSAGAIGSPQLLLSSGVGPKSHLSSLKLPVVLHNRHVGQSMADNPRFGAAIVL 302
           SKNGEIIL+AGAIGSP LLL SGVGPKSHLSSLKLPVVLHNRHVGQSMADNPRFGAAIVL
Sbjct: 270 SKNGEIILTAGAIGSPHLLLQSGVGPKSHLSSLKLPVVLHNRHVGQSMADNPRFGAAIVL 329

Query: 303 PFLTPPTSVQIVGTLKPNIHIESLSTILPFSISPPFALLPPRSAAVNLSLAIFAGKFSTV 362
           PFLTPPTSVQ+VGTLK NIHIESLS+ILPFSI PPF LLPPRS AVNLSLA+FAGKFSTV
Sbjct: 330 PFLTPPTSVQVVGTLKRNIHIESLSSILPFSIFPPFGLLPPRSTAVNLSLAVFAGKFSTV 389

Query: 363 SSTGSLRLDGRKNPIVRFNYLSHPDDLERCVEGVRKVGDLVNTKIMERIKTRDLEGKMGF 422
           SS GSLRLDGRKNPIVRFNYLSHPDD+ERCVEGVRKVGDLVNTK MERIKT DLEGK GF
Sbjct: 390 SSVGSLRLDGRKNPIVRFNYLSHPDDVERCVEGVRKVGDLVNTKSMERIKTGDLEGKKGF 449

Query: 423 EFLGSSLPENMSDYGLVGEFCRKTVTTFWHYHGGCLVGKVVDGNYKLIGIKNLRVVDGST 482
           +FLGS LPENMSDY LVG+FCRKTVTTFWHYHGGCLVGKVVD NY++IGIK LRVVDGST
Sbjct: 450 KFLGSPLPENMSDYKLVGDFCRKTVTTFWHYHGGCLVGKVVDDNYRVIGIKKLRVVDGST 509

Query: 483 FSLSPGTNPMATVMMLGRYVGLKMLEQRLG 513
           FSLSPGTNPMATVMMLGRYVGLKML+QRLG
Sbjct: 510 FSLSPGTNPMATVMMLGRYVGLKMLQQRLG 539

BLAST of Lsi11G007460 vs. NCBI nr
Match: XP_022997430.1 ((R)-mandelonitrile lyase 1-like [Cucurbita maxima])

HSP 1 Score: 913.7 bits (2360), Expect = 7.3e-262
Identity = 448/510 (87.84%), Postives = 481/510 (94.31%), Query Frame = 0

Query: 3   DDGYTKFVQNANTLPTTKEYDYIIVGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPLVL 62
           DD Y KFVQNANTLPT +EYDYII+GGGTAGCPLAATLSSNFSVLVLERGSDPNAFP VL
Sbjct: 30  DDSYMKFVQNANTLPTREEYDYIIIGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPSVL 89

Query: 63  SQEGMGNTLTDDDDGSNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFQNSGV 122
           SQ+G+ NTLTD+DDG NPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFQ+SGV
Sbjct: 90  SQQGLANTLTDNDDGLNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFQSSGV 149

Query: 123 QWDMTMVDEAYRWIEETVVSQPELGPWQSAFKEALVEAGVGPDNGYDLNHVVGTRIGGSV 182
           QWDM  V++AY+WIEETVVS+PEL PWQSAF+EAL+EAGVG DNGYDLNHVVGTR GGS+
Sbjct: 150 QWDMMAVEKAYQWIEETVVSRPELSPWQSAFREALLEAGVGRDNGYDLNHVVGTRTGGSI 209

Query: 183 FDSRGRRHGAVELLNKANSKNLKVATQATVKRIIFSQSNGLTATGVLYSDSKGKLHTATI 242
           FDS GRRHGAVELLNKA+ +NL+VATQATVKRIIFSQSNGL+A+GVLYSD KGKLH ATI
Sbjct: 210 FDSSGRRHGAVELLNKADPRNLRVATQATVKRIIFSQSNGLSASGVLYSDFKGKLHKATI 269

Query: 243 SKNGEIILSAGAIGSPQLLLSSGVGPKSHLSSLKLPVVLHNRHVGQSMADNPRFGAAIVL 302
           S+NGEIIL+AGAIGSP LLL SGVGP+SHLSSLKLPVV HN+HVGQ MADNPRFGAAIVL
Sbjct: 270 SRNGEIILTAGAIGSPHLLLQSGVGPRSHLSSLKLPVVFHNQHVGQFMADNPRFGAAIVL 329

Query: 303 PFLTPPTSVQIVGTLKPNIHIESLSTILPFSISPPFALLPPRSAAVNLSLAIFAGKFSTV 362
           PFLTPPTSVQ+VGTLKPNIHIESLS+ILPFSISPPF LLPPRS AVNLSLA+FAGKFSTV
Sbjct: 330 PFLTPPTSVQVVGTLKPNIHIESLSSILPFSISPPFGLLPPRSTAVNLSLAVFAGKFSTV 389

Query: 363 SSTGSLRLDGRKNPIVRFNYLSHPDDLERCVEGVRKVGDLVNTKIMERIKTRDLEGKMGF 422
           SS GSLRLDGRKNPIVRFNYLSHPDD++RCVEGVRKVGDLVNTK MERIKT DL+GK GF
Sbjct: 390 SSVGSLRLDGRKNPIVRFNYLSHPDDVQRCVEGVRKVGDLVNTKSMERIKTGDLDGKKGF 449

Query: 423 EFLGSSLPENMSDYGLVGEFCRKTVTTFWHYHGGCLVGKVVDGNYKLIGIKNLRVVDGST 482
           EFLG+ LPENMSDY LVG+FCRKTVTTFWHYHGGCLVGKVVD NY++IGIK LRVVDGST
Sbjct: 450 EFLGAPLPENMSDYRLVGDFCRKTVTTFWHYHGGCLVGKVVDDNYRVIGIKKLRVVDGST 509

Query: 483 FSLSPGTNPMATVMMLGRYVGLKMLEQRLG 513
           FSLSPGTNPMATVMMLGRYVGLKML+QRLG
Sbjct: 510 FSLSPGTNPMATVMMLGRYVGLKMLQQRLG 539

BLAST of Lsi11G007460 vs. TAIR 10
Match: AT1G73050.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 510.8 bits (1314), Expect = 1.3e-144
Identity = 252/515 (48.93%), Postives = 350/515 (67.96%), Query Frame = 0

Query: 5   GYTKFVQNANTLPTTKEYDYIIVGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPLVLSQ 64
           G+ +F+ NA    +   YDYIIVGGGTAGCPLAATLS +F VL+LERG  P   P V+S 
Sbjct: 37  GFMRFISNATDFASEDYYDYIIVGGGTAGCPLAATLSQSFRVLLLERGGVPYNRPNVMSH 96

Query: 65  EGMGNTLTDDDDGSNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFQNSGVQW 124
           +G   TLTD ++  +P Q F+SE+GV N RGRVLGG S IN GFYSRA  +FF+NSG+ W
Sbjct: 97  DGFLTTLTDVNNFDSPAQSFISEEGVPNARGRVLGGSSAINAGFYSRADKQFFENSGLVW 156

Query: 125 DMTMVDEAYRWIEETVVSQPELGPWQSAFKEALVEAGVGPDNGYDLNHVVGTRIGGSVFD 184
           D++ V+++Y W+E  +V +P+L  WQ+A ++AL+E GV P NG+ L H VGT+IGGS FD
Sbjct: 157 DLSSVNQSYEWVERAIVFRPQLRTWQTAIRDALLEVGVHPFNGFTLEHKVGTKIGGSTFD 216

Query: 185 SRGRRHGAVELLNKANSKNLKVATQATVKRIIFSQS-----NGLTATGVLYSDSKGKLHT 244
             GRRH + +LL  A S N++VA  ATV+R++ + S     + ++A GV+Y D  G+ H 
Sbjct: 217 RTGRRHSSADLLRYARSSNIRVAVYATVERVLLASSPSVSGSNVSAIGVVYRDQLGRFHH 276

Query: 245 ATISKNGEIILSAGAIGSPQLLLSSGVGPKSHLSSLKLPVVLHNRHVGQSMADNPRFGAA 304
           A I   GE+ILSAGA+GSPQLL  SG+GP+S+LS+  +PV L   HVG  + DNPR G +
Sbjct: 277 ALIRDRGEVILSAGALGSPQLLFLSGIGPRSYLSTWGIPVALDQPHVGDFVYDNPRNGIS 336

Query: 305 IVLPFLTPPTSVQIVGTLKPNIHIESLSTILPFSISPPFALLPPRSAAVNLSLAIFAGKF 364
           IV P     + +Q+VG  +    +E+ S ++PF+       +   ++ + + +     K 
Sbjct: 337 IVPPVPMENSLIQVVGVTEDGAFLEAASNVIPFASPLHSVFIRAPASPLYVPVTTIMEKI 396

Query: 365 STVSSTGSLRL---DGRKNPIVRFNYLSHPDDLERCVEGVRKVGDLVNTKIMERIKTRDL 424
               S G LRL   D R NP+VRFNY S P DLERCV G RK+G+++ ++ M+    R+ 
Sbjct: 397 LGPVSIGLLRLASTDVRINPVVRFNYFSDPQDLERCVNGTRKIGEILRSRAMQDFMIREW 456

Query: 425 EGKMGFEFLGSSLPENMSDYGLVGEFCRKTVTTFWHYHGGCLVGKVVDGNYKLIGIKNLR 484
            G   F F+G+ LP + S+  ++ +FCR+TV+T WHYHGG +VGKVVD + K+IG+ +LR
Sbjct: 457 FGNRRFRFVGAPLPVDQSNDLVMADFCRRTVSTIWHYHGGAVVGKVVDSDLKVIGVNSLR 516

Query: 485 VVDGSTFSLSPGTNPMATVMMLGRYVGLKMLEQRL 512
           +VDGSTF++SPGTNP AT+MMLGRY+GLKML +R+
Sbjct: 517 LVDGSTFNISPGTNPQATLMMLGRYMGLKMLRERM 551

BLAST of Lsi11G007460 vs. TAIR 10
Match: AT1G12570.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 427.2 bits (1097), Expect = 1.9e-119
Identity = 246/554 (44.40%), Postives = 328/554 (59.21%), Query Frame = 0

Query: 9   FVQNANTLPTTKEYDYIIVGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPLVLSQEGMG 68
           F+++A   PTT  YDYII+GGGTAGCPLAATLS N SVL+LERG  P   P +      G
Sbjct: 33  FMRDATGSPTTSYYDYIIIGGGTAGCPLAATLSQNASVLLLERGDSPYNNPNITRLSAFG 92

Query: 69  NTLTDDDDGSNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFQNSGVQWDMTM 128
             L+D  + S+P QRFVSEDGV N R RVLGGGS +N GFY+RA  ++ +N G  WD  +
Sbjct: 93  AALSDLSE-SSPSQRFVSEDGVINARARVLGGGSALNAGFYTRAGTKYVRNMG--WDGAL 152

Query: 129 VDEAYRWIEETVVSQPELGPWQSAFKEALVEAGVGPDNGYDLNHVVGTRIGGSVFDSRGR 188
            +E+Y+W+E  V  QP +G WQ+A ++ L+EAG+ P+NG+  +H+ GT+ GG++FD  G 
Sbjct: 153 ANESYQWVEAKVAFQPPMGRWQTAVRDGLLEAGIVPNNGFTYDHINGTKFGGTIFDRNGN 212

Query: 189 RHGAVELLNKANSKNLKVATQATVKRIIFSQSNGLT---ATGVLYSDSKGKLHTATISKN 248
           RH A +LL  A+ K + V   ATV RI+F ++ G T   A GV+Y D  G+ H A + + 
Sbjct: 213 RHTAADLLEYADPKGITVLLHATVHRILF-RTRGTTKPIANGVVYRDRTGQAHRAYLKEG 272

Query: 249 --GEIILSAGAIGSPQLLLSSGVGPKSHLSSLKLPVVLHNRHVGQSMADNPRFGAAIVLP 308
              EIILSAG +GSPQLL+ SGVGP + L +  + VV+   HVGQ M DNP     +  P
Sbjct: 273 ALSEIILSAGTLGSPQLLMLSGVGPSAQLQAQNITVVMDQPHVGQGMYDNPMNAVFVPSP 332

Query: 309 FLTPPTSVQIVGTLKPNIHIE----------------SLSTILPFSISPPFALLPPRSAA 368
                + +++VG      ++E                S ST   +++  P A L   ++ 
Sbjct: 333 VPVEVSLIEVVGITGEGTYVEAAGGENFGGGGGGSSGSSSTRDYYAMFSPRATLLESNSM 392

Query: 369 VNLSLA-IFAGKFSTVS-----STGSLRLDGRK---NPIVRFNYLSHPDDLERCVEGVRK 428
             LS A  F G F         STG L L  R    NPIV FNY  HPDDL+RCV G++ 
Sbjct: 393 TKLSSAQPFQGGFLLEKVMGPLSTGHLELKTRNPKDNPIVTFNYFQHPDDLKRCVRGIQT 452

Query: 429 VGDLVNTKIMERIKTRDLEGKMGFEFL------------------GSSLPENMSDYGLVG 488
           +  +V +K   R K  D    + FE+L                  G+SLP +        
Sbjct: 453 IERVVQSKAFSRYKYAD----VSFEYLLNLTASTPVNLRPPRSGPGASLPPSAE------ 512

Query: 489 EFCRKTVTTFWHYHGGCLVGKVVDGNYKLIGIKNLRVVDGSTFSLSPGTNPMATVMMLGR 515
           EFC+ TVTT WHYHGGC+VG+VVDG+YK+IGI  LRV+D ST    PGTNP ATVMMLGR
Sbjct: 513 EFCQHTVTTIWHYHGGCVVGRVVDGDYKVIGIDRLRVIDMSTVGYCPGTNPQATVMMLGR 572

BLAST of Lsi11G007460 vs. TAIR 10
Match: AT1G72970.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 409.5 bits (1051), Expect = 4.2e-114
Identity = 243/537 (45.25%), Postives = 320/537 (59.59%), Query Frame = 0

Query: 22  YDYIIVGGGTAGCPLAATLSSNFSVLVLERGSDP--NAFPLVLSQEGMGNTLTDDDDGSN 81
           YDYI++GGGTAGCPLAATLS NFSVLVLERG  P  NA    L    +G     D   S+
Sbjct: 63  YDYIVIGGGTAGCPLAATLSQNFSVLVLERGGVPFTNANVSFLRNFHIG---LADISASS 122

Query: 82  PFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFQNSGVQWDMTMVDEAYRWIEET 141
             Q FVS DGV N R RVLGGGS IN GFYSRA   F + +G  WD  +V E+Y W+E  
Sbjct: 123 ASQAFVSTDGVYNARARVLGGGSCINAGFYSRADAAFVKRAG--WDPKLVKESYPWVERE 182

Query: 142 VVSQPELGPWQSAFKEALVEAGVGPDNGYDLNHVVGTRIGGSVFDSRGRRHGAVELLNKA 201
           +V QP+L  WQ A +++L+E GV P NG+  +HV GT+IGG++FD  GRRH A ELL  A
Sbjct: 183 IVHQPKLTLWQKALRDSLLEVGVRPFNGFTYDHVSGTKIGGTIFDRFGRRHTAAELLAYA 242

Query: 202 NSKNLKVATQATVKRIIFSQSNGL-TATGVLYSDSKGKLHTATIS--KNGEIILSAGAIG 261
           N + L+V   ATV++I+F  S      TGV++ D KG  H A +S  K  E+ILS+GAIG
Sbjct: 243 NPQKLRVLIYATVQKIVFDTSGTRPRVTGVIFKDEKGNQHQALLSNRKGSEVILSSGAIG 302

Query: 262 SPQLLLSSGVGPKSHLSSLKLPVVLHNRHVGQSMADNPRFGAAIVLPFLTP--PTSVQIV 321
           SPQ+L+ SG+GPK  L  LK+PVVL N HVG+ MADNP     I++P   P   + +Q V
Sbjct: 303 SPQMLMLSGIGPKKELQRLKIPVVLENEHVGKGMADNPM--NTILVPSKAPIEQSLIQTV 362

Query: 322 GTLKPNIHIESLSTI--LPFSI----------SPPFALLPPRSAAVNLSLA--------- 381
           G  K  +++E+ +     P SI          +  F+ +P +      + A         
Sbjct: 363 GITKMGVYVEASTGFGQSPESIHTHYGIMSNKNELFSTIPAKQRRPEATQAYITRNKYQL 422

Query: 382 --IFAGKF-----STVSSTGSLRLDGRK---NPIVRFNYLSHPDDLERCVEGVRKVGDLV 441
              F G F     +   S G L L       NP V FNY  HP DL+RCVE +R V  +V
Sbjct: 423 HEAFNGSFILEKLAYPISRGHLSLVNTNVDDNPSVTFNYFKHPVDLQRCVEAIRLVSKVV 482

Query: 442 NTKIMERIKTRDLEG--KMGFEFLGSSL---PENMSDYGLVGEFCRKTVTTFWHYHGGCL 501
            +         D +   KM    + +++   P+ ++D   + +FC+ TV T WHYHGGCL
Sbjct: 483 TSNRFLNYTQCDKQNVHKMLSLSVKANINLRPKQLNDTKSMAQFCKDTVVTIWHYHGGCL 542

Query: 502 VGKVVDGNYKLIGIKNLRVVDGSTFSLSPGTNPMATVMMLGRYVGLKMLEQRLGRRS 516
           VGKVV  N K++G+  LRV+DGSTF  SPGTNP AT+MM+GRY+G+K+L +RLG ++
Sbjct: 543 VGKVVSPNRKVLGVDRLRVIDGSTFDESPGTNPQATMMMMGRYMGVKILRERLGNKA 592

BLAST of Lsi11G007460 vs. TAIR 10
Match: AT1G72970.2 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 391.7 bits (1005), Expect = 9.0e-109
Identity = 235/532 (44.17%), Postives = 308/532 (57.89%), Query Frame = 0

Query: 22  YDYIIVGGGTAGCPLAATLSSNFSVLVLERGSDP--NAFPLVLSQEGMGNTLTDDDDGSN 81
           YDYI++GGGTAGCPLAATLS NFSVLVLERG  P  NA    L    +G     D   S+
Sbjct: 63  YDYIVIGGGTAGCPLAATLSQNFSVLVLERGGVPFTNANVSFLRNFHIG---LADISASS 122

Query: 82  PFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFQNSGVQWDMTMVDEAYRWIEET 141
             Q FVS DGV N R RVLGGGS IN GFYSRA   F + +G  WD  +V E+Y W+E  
Sbjct: 123 ASQAFVSTDGVYNARARVLGGGSCINAGFYSRADAAFVKRAG--WDPKLVKESYPWVERE 182

Query: 142 VVSQPELGPWQSAFKEALVEAGVGPDNGYDLNHVVGTRIGGSVFDSRGRRHGAVELLNKA 201
           +V QP+L  WQ A +++L+E GV P NG+  +HV GT+IGG++FD  GRRH A ELL  A
Sbjct: 183 IVHQPKLTLWQKALRDSLLEVGVRPFNGFTYDHVSGTKIGGTIFDRFGRRHTAAELLAYA 242

Query: 202 NSKNLKVATQATVKRIIFSQSNGL-TATGVLYSDSKGKLHTATIS--KNGEIILSAGAIG 261
           N + L+V   ATV++I+F  S      TGV++ D KG  H A +S  K  E+ILS+GAIG
Sbjct: 243 NPQKLRVLIYATVQKIVFDTSGTRPRVTGVIFKDEKGNQHQALLSNRKGSEVILSSGAIG 302

Query: 262 SPQLLLSSGVGPKSHLSSLKLPVVLHNRHVGQSMADNPRFGAAIVLPFLTP--PTSVQIV 321
           SPQ+L+ SG+GPK  L  LK+PVVL N HVG+ MADNP     I++P   P   + +Q V
Sbjct: 303 SPQMLMLSGIGPKKELQRLKIPVVLENEHVGKGMADNPM--NTILVPSKAPIEQSLIQTV 362

Query: 322 GTLKPNIHIESLSTI--LPFSI----------SPPFALLPPRSAAVNLSLA--------- 381
           G  K  +++E+ +     P SI          +  F+ +P +      + A         
Sbjct: 363 GITKMGVYVEASTGFGQSPESIHTHYGIMSNKNELFSTIPAKQRRPEATQAYITRNKYQL 422

Query: 382 --IFAGKF-----STVSSTGSLRLDGRK---NPIVRFNYLSHPDDLERCVEGVRKVGDLV 441
              F G F     +   S G L L       NP V FNY  HP D +        V  ++
Sbjct: 423 HEAFNGSFILEKLAYPISRGHLSLVNTNVDDNPSVTFNYFKHPCDKQ-------NVHKML 482

Query: 442 NTKIMERIKTRDLEGKMGFEFLGSSLPENMSDYGLVGEFCRKTVTTFWHYHGGCLVGKVV 501
           +  +   I  R               P+ ++D   + +FC+ TV T WHYHGGCLVGKVV
Sbjct: 483 SLSVKANINLR---------------PKQLNDTKSMAQFCKDTVVTIWHYHGGCLVGKVV 542

Query: 502 DGNYKLIGIKNLRVVDGSTFSLSPGTNPMATVMMLGRYVGLKMLEQRLGRRS 516
             N K++G+  LRV+DGSTF  SPGTNP AT+MM+GRY+G+K+L +RLG ++
Sbjct: 543 SPNRKVLGVDRLRVIDGSTFDESPGTNPQATMMMMGRYMGVKILRERLGNKA 565

BLAST of Lsi11G007460 vs. TAIR 10
Match: AT5G51950.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 388.7 bits (997), Expect = 7.6e-108
Identity = 232/553 (41.95%), Postives = 313/553 (56.60%), Query Frame = 0

Query: 5   GYTKFVQNANTLPTTKEYDYIIVGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPLVLSQ 64
           GY  F+++A   P    +DYII+GGGT+GC LAATLS N SVLVLERG  P   P     
Sbjct: 29  GYYSFMKDATLAPMFARFDYIIIGGGTSGCALAATLSQNASVLVLERGGAPYDNPTATDI 88

Query: 65  EGMGNTLTDDDDGSNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFQNSGVQW 124
           E    TL++    S   Q F+SEDGV N R RVLGGGS++N GFY+RA  E+ + +  +W
Sbjct: 89  ENFATTLSNTSPKSWS-QLFISEDGVYNTRARVLGGGSVLNAGFYTRAGDEYVKET--EW 148

Query: 125 DMTMVDEAYRWIEETVVSQPELGPWQSAFKEALVEAGVGPDNGYDLNHVVGTRIGGSVFD 184
               V+ AY W+E+ V  QP +  WQ+AFK+ L+EAG  P NG+  +H+ GT+IGG++FD
Sbjct: 149 KTDEVEAAYEWVEKKVAFQPPVLGWQTAFKDGLLEAGEFPYNGFTYDHIYGTKIGGTIFD 208

Query: 185 SRGRRHGAVELLNKANSKNLKVATQATVKRIIFSQSN--GLTATGVLYSDSKGKLHTATI 244
             G RH A +LL  AN  N+ V   A+V +I+F+        A GV++ D+ G LH A +
Sbjct: 209 RAGHRHTAADLLEYANPGNIVVYLHASVHKILFTTKGRPRPKAYGVIFQDANGVLHKAEL 268

Query: 245 SKN--GEIILSAGAIGSPQLLLSSGVGPKSHLSSLKL-PVVLHNRHVGQSMADNPRFGAA 304
            KN   E+ILSAGAIGSPQLL+ SG+GP +HL++  + P+VL +  VGQ M DNP     
Sbjct: 269 EKNSMNEVILSAGAIGSPQLLMLSGIGPAAHLAAHGIKPLVLDHPMVGQGMGDNPMNAIF 328

Query: 305 IVLPFLTPPTSVQIVGTLKPNIHIESLS-------------------------------- 364
           I  P     + +Q+VG  K   +IE  S                                
Sbjct: 329 IPSPTPVEVSLIQVVGITKFESYIEGASGVIFSYSWTRRFFDGVLNYLNEMQTSRTTSTT 388

Query: 365 --TILPFSISPPFALLPPRSAAVNLSLAIFAGKFSTVSSTGSLRLDGRK---NPIVRFNY 424
             T+   SI+  F  + P   A   +  +   K +   S G L L       NP VRFNY
Sbjct: 389 SPTLSTQSITDFFNPINPLLNATTRA-GLILQKIAGPISRGHLELRNTNPDDNPSVRFNY 448

Query: 425 LSHPDDLERCVEGVRKVGDLVNTKIMERIKTRDLEGKMGFEFLGSSLPENMSDYGLVG-- 484
              P+DL+ CVEG+  +  ++N+K   + K  D     G   L  S+P N+    +    
Sbjct: 449 YQEPEDLQICVEGINTIIKVINSKAFSKFKYPDAT-IHGLLDLMLSVPTNLRPRHITSMF 508

Query: 485 ---EFCRKTVTTFWHYHGGCLVGKVVDGNYKLIGIKNLRVVDGSTFSLSPGTNPMATVMM 511
              +FC  TV T WHYHGGC VG+VVD NY+++GI +LRV+DGSTF  SPGTNP ATVMM
Sbjct: 509 NLRQFCIDTVMTIWHYHGGCQVGRVVDKNYRVLGIDSLRVIDGSTFLKSPGTNPQATVMM 568

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P527061.1e-14350.68(R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1[more]
Q9SSM21.9e-14348.93(R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 ... [more]
Q945K21.7e-14150.29(R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1[more]
O827848.1e-13950.19(R)-mandelonitrile lyase 4 OS=Prunus serotina OX=23207 GN=MDL4 PE=2 SV=1[more]
P527071.6e-13448.25(R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7UKP14.2e-27992.26(R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A1S3BTC34.2e-27992.26(R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493044 PE=3 SV=... [more]
A0A0A0LMC22.7e-27892.07GMC_OxRdtase_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G057... [more]
A0A6J1EN541.6e-26288.24(R)-mandelonitrile lyase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111436125 PE... [more]
A0A6J1K9L83.5e-26287.84(R)-mandelonitrile lyase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111492348 PE=3... [more]
Match NameE-valueIdentityDescription
XP_038890837.12.9e-27992.28(R)-mandelonitrile lyase 1-like [Benincasa hispida][more]
XP_008451889.18.6e-27992.26PREDICTED: (R)-mandelonitrile lyase 1-like [Cucumis melo] >KAA0055628.1 (R)-mand... [more]
XP_011648924.15.6e-27892.07(R)-mandelonitrile lyase 1 [Cucumis sativus][more]
XP_022929582.13.3e-26288.24(R)-mandelonitrile lyase 1-like [Cucurbita moschata][more]
XP_022997430.17.3e-26287.84(R)-mandelonitrile lyase 1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT1G73050.11.3e-14448.93Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G12570.11.9e-11944.40Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G72970.14.2e-11445.25Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G72970.29.0e-10944.17Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT5G51950.17.6e-10841.95Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012132Glucose-methanol-choline oxidoreductasePIRSFPIRSF000137Alcohol_oxidasecoord: 8..310
e-value: 1.2E-59
score: 200.6
coord: 349..516
e-value: 3.9E-26
score: 89.9
IPR007867Glucose-methanol-choline oxidoreductase, C-terminalPFAMPF05199GMC_oxred_Ccoord: 364..500
e-value: 1.6E-25
score: 90.2
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 22..499
e-value: 2.2E-162
score: 543.4
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 17..508
NoneNo IPR availableGENE3D3.30.410.40coord: 142..449
e-value: 2.2E-162
score: 543.4
NoneNo IPR availablePIRSRPIRSR000137-2PIRSR000137-2coord: 360..510
e-value: 2.6E-34
score: 116.7
NoneNo IPR availablePANTHERPTHR45968OSJNBA0019K04.7 PROTEINcoord: 3..511
NoneNo IPR availablePANTHERPTHR45968:SF23(R)-MANDELONITRILE LYASE 4-RELATEDcoord: 3..511
NoneNo IPR availableSUPERFAMILY54373FAD-linked reductases, C-terminal domaincoord: 352..456
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPFAMPF00732GMC_oxred_Ncoord: 22..294
e-value: 1.2E-35
score: 123.3
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPROSITEPS00624GMC_OXRED_2coord: 253..267
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPROSITEPS00623GMC_OXRED_1coord: 95..118

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi11G007460.1Lsi11G007460.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016829 lyase activity
molecular_function GO:0016614 oxidoreductase activity, acting on CH-OH group of donors