Lsi11G007430 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi11G007430
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	(R)-mandelonitrile lyase 1-like
Location	chr11: 8161008 .. 8164070 (-)
RNA-Seq Expression	Lsi11G007430
Synteny	Lsi11G007430

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTCAATGCTGGCTTTTACTCGAGGGGCCATCAAGAGTTCTTTGAAAGAGCAGGTGTTGATTGGGACATGGAAATGGTGGAGAAGGCTTATGAATGGGTTGAAGAAACTGTGGTATCTAGGCCAAGTTTGAGTGGTTGGCAATTTGCTTTTAGGAGTGCGTTGTTGGAAGGTGGGGTTGGTCCTGATAATGGGTTTGATTTGAGGCATATTGTGGGAACTAAAACTGGAGGTTCCATCTTTGATGACAAAGGAAACAGACATGGAGCTGTGGAGTTTCTTAATAAGGCTAATCCCAGAAATCTTAAAGTTGTCATTCAAGCCACAGTTCAAAGGATGCATCTACTGTCTTTGGGAGCATGATGAGGGTGTTAAGGATGTGTCAACCTAGTTGAGATATCCTGGTGCACCTACTGATCCCTCTCTAGTACCTTTGTTAAAAAAAAAAGCCACAGTTCAAAGGATCATCTTCACTGGCTAGTGTTTTATAAGGAAAATTTGGTTTAAAGTTTTTGTTGGTCAATGAATTTTTTGAAAATGGTTCCTCAACTTATGCACCATTTACCTAATCTTCAACTTTTAAAAAGAGAAGAAAAAGTACAATTTTGATATTGATATTAGAGAAAGAATAATACTTCTAGTGCATTCTAAGATGTTGTTTATGTGTATTCTGTAATTGTAATGAATTGTGGGGCACACTTCCAAATCTGTAATCAAAACATGTAATCTGATTGAAGAATTGAGGGTGCTTAATCTGATTGTGGTTGAAAGTTATAACTAGGACCCATTACCTTTATCGTTATAGATATTGTTAGTGTAACAATATGAAATTAATATATCGCTATTGACATCGGGGCATGATGAGGGTGCTACGGAGGTGTCAACCTAGTTGAGATGTCCTGGTGCACCCCCTGATCCAACTTTCCATTGGTTACTTGTTCAAAAAAAAAAAGTATGAAAATTATGAATATATTAAATTTATTGTTACTTTTACCTTTACCATTATCGTTAGGGTCAAGCAGTAATGGGAATGGTAACGATGACAGTAAAACGTGACAGATTTATTATTCTAAGTAAACGCGATCAAAAGCAATCAAATACATTTGCGATTTAAGCCCAATACAATTGATCTATCAATGAAATCTCATTATGATTACATCAATTTTCATTACAGATTGATAAACGTAACCTAAACTTAATCCACAAAAATAAGTTTCTTGAAATAATTTTTTTTTTTTTTTTGGTTTTTAAGAGTAAACTTAAGATGCGTTTGGATTGACTTTTTAAGTGTTTAAACACTTAGAAAGTTAATCCAAACAAGTTATTGCATCGACAAAAACACCCAAATTTCAAACTTTTTTCTATTTGGAATTTTATAGGTTTATCTGCAAGTGGAGTATCATATTCAGATTCAAAAGGAAAGTTGCATAGAGCATTCATTCGTAAGAAAGGGGAGATTATTTTAAGTGCTGGAGCCATTAGAAGTCCTCAACTTCTCATTCTAAGTGGGGTTGCCTCAAAATCTCATCTTTCATCTTTAAAACTACCTGTCGTTCTCCACCAACCACATGTCGGTCAATTCATGACTGAATCCTCGTTTCAGTCCCACCATCGTTCTTCCATTCCAATTAGTTGCTTCATCTGCACAAGTCGTTGGAACATTAGACAACAATATCCATTTACAATTATTCGCCAGCCCTTTACCATTCTTCCCTCTACCATCTTATAGCCTTCTTCCTTCTCAATCCACTTCCATCACTCCTAGCTTAGCCATTTTTGTTGGAAAATTCTCTAATGTTCATTCTAAGGGGTGGCTCCACTTGAATTCTTCCACTGATGTGAAGGAGAGTCCCATTGTTCGATTCAATTATTATTCTCATCATCATGATCTTACTCGATGTGTTAGAGGAGTAAGAAAAGTGGGAGATTTGCTTAAAACTCAAACCATGGAAAAGATTAAGACAGAAGATTTGGAGGGTAATAAAGGATTTGAATTTATGGAGCTTCCAATGCCGGAAAATTTGTGGAATGATAGTCTGGTTGAAGAATATTGTCGAAACACGGTGGCTACTTATTGGCATTACCATGGAGGATGTTTGGTCGGAAAAGTGGTCGATGATAATTATAAAGTTATTGGAATTAAAAATTTGCGTGTTGTGGATGGCTCCACCTTCTCCGACTCACCGGGAACTAATCCTATGGCCACCCTTATGATGCTGGGCCGGTGGGTTTTTTTTTTCTTTTATATAAAAAAAAAATGTTAAAGATTTATAAGATATAACTTTTTGAAAATTGCGAGATCACCAAACATGTAATTCAACTTAACTTTTTCGTTTTTCTAATATTATAGAAATATTGTCTTTTAAATTGTTTGAACCTTGATACACCTTTTTAAGAACGAAGTATTTTTGTTGGAAAACTTTTTTTTTAATGATTAATAATAGCATAATGTTTTCTTAGAGGAAAATAATACTACATTTCATGTATTGATTGTACTTGGAATTATGTATATGGGTAGTACGTACTCGGTCTTGGAAAAATCAAAGGATCTCACACTTTTAATAAGAAAGTTGGGTTACTCCTCTTATTGTCAATTGGTTTTGAGATGGAACACTATCCGATAGTACTATTGTATTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATTATGTATTGGTTGCACTAGAGATTTTTTTTTATTATTATTATATATCTTTTATTTCCATTAACTTACCATTTCAAAATTGTTTTGCAGCTATGTTGGCCTTAAGTAAACTAGGATAGATCAATATTCCACACTTCTTTCAGTATAATATAAGGAATTGAGAGATTTGAATCAAATACTTCATGATCACAGTATGTAA

mRNA sequence

ATGGTCAATGCTGGCTTTTACTCGAGGGGCCATCAAGAGTTCTTTGAAAGAGCAGGTGTTGATTGGGACATGGAAATGGTGGAGAAGGCTTATGAATGGGTTGAAGAAACTGTGGTATCTAGGCCAAGTTTGAGTGGTTGGCAATTTGCTTTTAGGAGTGCGTTGTTGGAAGGTGGGGTTGGTCCTGATAATGGGTTTGATTTGAGGCATATTGTGGGAACTAAAACTGGAGGTTTATCTGCAAGTGGAGTATCATATTCAGATTCAAAAGGAAAGTTGCATAGAGCATTCATTCGTAAGAAAGGGGAGATTATTTTAAGTGCTGGAGCCATTAGAAGTCCTCAACTTCTCATTCTAATTGCTTCATCTGCACAAGTCGTTGGAACATTAGACAACAATATCCATTTACAATTATTCGCCAGCCCTTTACCATTCTTCCCTCTACCATCTTATAGCCTTCTTCCTTCTCAATCCACTTCCATCACTCCTAGCTTAGCCATTTTTGTTGGAAAATTCTCTAATGTTCATTCTAAGGGGTGGCTCCACTTGAATTCTTCCACTGATGTGAAGGAGAGTCCCATTGTTCGATTCAATTATTATTCTCATCATCATGATCTTACTCGATGTGTTAGAGGAGTAAGAAAAGTGGGAGATTTGCTTAAAACTCAAACCATGGAAAAGATTAAGACAGAAGATTTGGAGGGTAATAAAGGATTTGAATTTATGGAGCTTCCAATGCCGGAAAATTTGTGGAATGATAGTCTGGTTGAAGAATATTGTCGAAACACGGTGGCTACTTATTGGCATTACCATGGAGGATGTTTGGTCGGAAAAGTGGTCGATGATAATTATAAAGTTATTGGAATTAAAAATTTGCGTGTTGTGGATGGCTCCACCTTCTCCGACTCACCGGGAACTAATCCTATGGCCACCCTTATGATGCTGGGCCGCTATGTTGGCCTTAATATGTAA

Coding sequence (CDS)

ATGGTCAATGCTGGCTTTTACTCGAGGGGCCATCAAGAGTTCTTTGAAAGAGCAGGTGTTGATTGGGACATGGAAATGGTGGAGAAGGCTTATGAATGGGTTGAAGAAACTGTGGTATCTAGGCCAAGTTTGAGTGGTTGGCAATTTGCTTTTAGGAGTGCGTTGTTGGAAGGTGGGGTTGGTCCTGATAATGGGTTTGATTTGAGGCATATTGTGGGAACTAAAACTGGAGGTTTATCTGCAAGTGGAGTATCATATTCAGATTCAAAAGGAAAGTTGCATAGAGCATTCATTCGTAAGAAAGGGGAGATTATTTTAAGTGCTGGAGCCATTAGAAGTCCTCAACTTCTCATTCTAATTGCTTCATCTGCACAAGTCGTTGGAACATTAGACAACAATATCCATTTACAATTATTCGCCAGCCCTTTACCATTCTTCCCTCTACCATCTTATAGCCTTCTTCCTTCTCAATCCACTTCCATCACTCCTAGCTTAGCCATTTTTGTTGGAAAATTCTCTAATGTTCATTCTAAGGGGTGGCTCCACTTGAATTCTTCCACTGATGTGAAGGAGAGTCCCATTGTTCGATTCAATTATTATTCTCATCATCATGATCTTACTCGATGTGTTAGAGGAGTAAGAAAAGTGGGAGATTTGCTTAAAACTCAAACCATGGAAAAGATTAAGACAGAAGATTTGGAGGGTAATAAAGGATTTGAATTTATGGAGCTTCCAATGCCGGAAAATTTGTGGAATGATAGTCTGGTTGAAGAATATTGTCGAAACACGGTGGCTACTTATTGGCATTACCATGGAGGATGTTTGGTCGGAAAAGTGGTCGATGATAATTATAAAGTTATTGGAATTAAAAATTTGCGTGTTGTGGATGGCTCCACCTTCTCCGACTCACCGGGAACTAATCCTATGGCCACCCTTATGATGCTGGGCCGCTATGTTGGCCTTAATATGTAA

Protein sequence

MVNAGFYSRGHQEFFERAGVDWDMEMVEKAYEWVEETVVSRPSLSGWQFAFRSALLEGGVGPDNGFDLRHIVGTKTGGLSASGVSYSDSKGKLHRAFIRKKGEIILSAGAIRSPQLLILIASSAQVVGTLDNNIHLQLFASPLPFFPLPSYSLLPSQSTSITPSLAIFVGKFSNVHSKGWLHLNSSTDVKESPIVRFNYYSHHHDLTRCVRGVRKVGDLLKTQTMEKIKTEDLEGNKGFEFMELPMPENLWNDSLVEEYCRNTVATYWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLNM

Homology

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BLAST of Lsi11G007430 vs. ExPASy Swiss-Prot
Match: Q9SSM2 ((R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 SV=1)

HSP 1 Score: 280.0 bits (715), Expect = 3.3e-74
Identity = 166/413 (40.19%), Postives = 217/413 (52.54%), Query Frame = 0


Query: 2   VNAGFYSRGHQEFFERAGVDWDMEMVEKAYEWVEETVVSRPSLSGWQFAFRSALLEGGVG 61
           +NAGFYSR  ++FFE +G+ WD+  V ++YEWVE  +V RP L  WQ A R ALLE GV 
Sbjct: 136 INAGFYSRADKQFFENSGLVWDLSSVNQSYEWVERAIVFRPQLRTWQTAIRDALLEVGVH 195

Query: 62  PDNGFDLRHIVGTKTGG------------------------------------------- 121
           P NGF L H VGTK GG                                           
Sbjct: 196 PFNGFTLEHKVGTKIGGSTFDRTGRRHSSADLLRYARSSNIRVAVYATVERVLLASSPSV 255

Query: 122 ----LSASGVSYSDSKGKLHRAFIRKKGEIILSAGAIRSPQLLILIA------------- 181
               +SA GV Y D  G+ H A IR +GE+ILSAGA+ SPQLL L               
Sbjct: 256 SGSNVSAIGVVYRDQLGRFHHALIRDRGEVILSAGALGSPQLLFLSGIGPRSYLSTWGIP 315

Query: 182 ------------------------------SSAQVVGTLDNNIHLQLFASPLPF-FPLPS 241
                                         S  QVVG  ++   L+  ++ +PF  PL S
Sbjct: 316 VALDQPHVGDFVYDNPRNGISIVPPVPMENSLIQVVGVTEDGAFLEAASNVIPFASPLHS 375

Query: 242 YSLLPSQSTSITPSLAIFVGKFSNVHSKGWLHLNSSTDVKESPIVRFNYYSHHHDLTRCV 301
             +    S    P   I + K     S G L L +STDV+ +P+VRFNY+S   DL RCV
Sbjct: 376 VFIRAPASPLYVPVTTI-MEKILGPVSIGLLRL-ASTDVRINPVVRFNYFSDPQDLERCV 435

Query: 302 RGVRKVGDLLKTQTMEKIKTEDLEGNKGFEFMELPMPENLWNDSLVEEYCRNTVATYWHY 324
            G RK+G++L+++ M+     +  GN+ F F+  P+P +  ND ++ ++CR TV+T WHY
Sbjct: 436 NGTRKIGEILRSRAMQDFMIREWFGNRRFRFVGAPLPVDQSNDLVMADFCRRTVSTIWHY 495

[Close]

BLAST of Lsi11G007430 vs. ExPASy Swiss-Prot
Match: P52707 ((R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1)

HSP 1 Score: 274.6 bits (701), Expect = 1.4e-72
Identity = 154/410 (37.56%), Postives = 215/410 (52.44%), Query Frame = 0


Query: 1   MVNAGFYSRGHQEFFERAGVDWDMEMVEKAYEWVEETVVSRPSLSGWQFAFRSALLEGGV 60
           M+NAG Y R +  FF + G++WDM++V + YEWVE+T+V  P    WQ    +A LE G+
Sbjct: 135 MINAGVYVRANTSFFNQTGIEWDMDLVNQTYEWVEDTIVFEPDSQTWQTVIGTAYLEAGI 194

Query: 61  GPDNGFDLRHIVGTK-------------------------------------------TG 120
            P+NGF + H+ GT+                                           T 
Sbjct: 195 LPNNGFSVDHLAGTRLTGSTFDNNGTRHASDELLNKGDPNNLRVAVQAAVEKIIFSSNTS 254

Query: 121 GLSASGVSYSDSKGKLHRAFIRKKGEIILSAGAIRSPQLLILI----------------- 180
           G++A GV Y+DS G  H+AF+R +GE+ILSAG I SPQLL+L                  
Sbjct: 255 GVTAIGVIYTDSNGTTHQAFVRGEGEVILSAGPIGSPQLLLLSGVGPESYLTSLNISVVA 314

Query: 181 --------------------------ASSAQVVGTLDNNIHLQLFASPLPFFPLPSYSLL 240
                                     AS+  V+G   +    Q   S LP F  P +S  
Sbjct: 315 SHPYVGQYIYDNPRNFINILPPNPIEASTVTVLGITSD--FYQCSISSLP-FDTPPFSFF 374

Query: 241 PSQSTSI-TPSLAIFVGKFSNVHSKGWLHLNSSTDVKESPIVRFNYYSHHHDLTRCVRGV 300
           P+ S  +   + A  V K     S G + LNSS+DV+  P V+FNYYS+  DL+ CV G+
Sbjct: 375 PTTSYPLPNQTFAHIVNKVPGPLSHGTVTLNSSSDVRVGPNVKFNYYSNLTDLSHCVSGM 434

Query: 301 RKVGDLLKTQTMEKIKTEDLEGNKGFEFMELPMPENLWNDSLVEEYCRNTVATYWHYHGG 324
           +K+G++L T  +E  K EDL G  GF  + +P+PEN  +D+  E +CR +VA+YWHYHGG
Sbjct: 435 KKLGEVLSTDALEPYKVEDLPGIDGFNILGIPLPENQTDDAAFETFCRESVASYWHYHGG 494

[Close]

BLAST of Lsi11G007430 vs. ExPASy Swiss-Prot
Match: P52706 ((R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 4.1e-72
Identity = 160/407 (39.31%), Postives = 219/407 (53.81%), Query Frame = 0


Query: 1   MVNAGFYSRGHQEFFERAGVDWDMEMVEKAYEWVEETVVSRPSLSGWQFAFRSALLEGGV 60
           M+NAG Y+R +   +  +GVDWDM++V K YEWVE+T+V +P+   WQ    +A LE GV
Sbjct: 135 MINAGVYARANTSIYSASGVDWDMDLVNKTYEWVEDTIVFKPNYQPWQSVTGTAFLEAGV 194

Query: 61  GPDNGFDLRHIVGTK-TG-----------------------------------------G 120
            P++GF L H  GT+ TG                                         G
Sbjct: 195 DPNHGFSLDHEAGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGVHASVEKIIFSNAPG 254

Query: 121 LSASGVSYSDSKGKLHRAFIRKKGEIILSAGAIRSPQLLIL---------------IASS 180
           L+A+GV Y DS G  HRAF+R KGE+I+SAG I +PQLL+L               +  S
Sbjct: 255 LTATGVIYRDSNGTPHRAFVRSKGEVIVSAGTIGTPQLLLLSGVGPESYLSSLNIPVVLS 314

Query: 181 AQVVGTL--DN------------------------NIHLQLFASPLPFFPLPSYSLLPSQ 240
              VG    DN                        N   Q   S LP F  P +S  PS 
Sbjct: 315 HPYVGQFLHDNPRNFINILPPNPIEPTIVTVLGISNDFYQCSFSSLP-FTTPPFSFFPST 374

Query: 241 STSITPS-LAIFVGKFSNVHSKGWLHLNSSTDVKESPIVRFNYYSHHHDLTRCVRGVRKV 300
           S  +  S  A F  K +   S G L L SS++V+ SP V+FNYYS+  DL+ CV G++K+
Sbjct: 375 SYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYYSNPTDLSHCVSGMKKI 434

Query: 301 GDLLKTQTMEKIKTEDLEGNKGFEFMELPMPENLWNDSLVEEYCRNTVATYWHYHGGCLV 324
           G+LL T  ++  K EDL G +GF  + +P+P++  +D+  E +CR +VA+YWHYHGGCLV
Sbjct: 435 GELLSTDALKPYKVEDLPGIEGFNILGIPLPKDQTDDAAFETFCRESVASYWHYHGGCLV 494

[Close]

BLAST of Lsi11G007430 vs. ExPASy Swiss-Prot
Match: Q945K2 ((R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 1.7e-70
Identity = 156/407 (38.33%), Postives = 219/407 (53.81%), Query Frame = 0


Query: 1   MVNAGFYSRGHQEFFERAGVDWDMEMVEKAYEWVEETVVSRPSLSGWQFAFRSALLEGGV 60
           ++NAG Y+R +   +  +GVDWDM++V + YEWVE+T+V +P+   WQ   ++A LE GV
Sbjct: 135 IINAGVYARANTSIYSASGVDWDMDLVNQTYEWVEDTIVYKPNSQSWQSVTKTAFLEAGV 194

Query: 61  GPDNGFDLRHIVGTK-TG-----------------------------------------G 120
            P++GF L H  GT+ TG                                         G
Sbjct: 195 HPNHGFSLDHEEGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGVHASVEKIIFSNAPG 254

Query: 121 LSASGVSYSDSKGKLHRAFIRKKGEIILSAGAIRSPQLLIL---------------IASS 180
           L+A+GV Y DS G  H+AF+R KGE+I+SAG I +PQLL+L               +  S
Sbjct: 255 LTATGVIYRDSNGTPHQAFVRSKGEVIVSAGTIGTPQLLLLSGVGPESYLSSLNIPVVLS 314

Query: 181 AQVVGTL--DN------------------------NIHLQLFASPLPFFPLPSYSLLPSQ 240
              VG    DN                        N   Q   S LP F  P +   PS 
Sbjct: 315 HPYVGQFLHDNPRNFINILPPNPIEPTIVTVLGISNDFYQCSFSSLP-FTTPPFGFFPSA 374

Query: 241 STSITPS-LAIFVGKFSNVHSKGWLHLNSSTDVKESPIVRFNYYSHHHDLTRCVRGVRKV 300
           S  +  S  A F  K +   S G L L SS++V+ SP V+FNYYS+  DL+ CV G++K+
Sbjct: 375 SYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYYSNLTDLSHCVSGMKKI 434

Query: 301 GDLLKTQTMEKIKTEDLEGNKGFEFMELPMPENLWNDSLVEEYCRNTVATYWHYHGGCLV 324
           G+LL T  ++  K EDL G +GF  + +P+P++  +D+  E +CR +VA+YWHYHGGCLV
Sbjct: 435 GELLSTDALKPYKVEDLPGVEGFNILGIPLPKDQTDDAAFETFCRESVASYWHYHGGCLV 494

[Close]

BLAST of Lsi11G007430 vs. ExPASy Swiss-Prot
Match: O24243 ((R)-mandelonitrile lyase 1 OS=Prunus dulcis OX=3755 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 1.4e-69
Identity = 151/410 (36.83%), Postives = 215/410 (52.44%), Query Frame = 0


Query: 1   MVNAGFYSRGHQEFFERAGVDWDMEMVEKAYEWVEETVVSRPSLSGWQFAFRSALLEGGV 60
           ++NAG Y+R +  F+ + G++WD+++V K YEWVE+ +V +P+   WQ       LE G+
Sbjct: 135 IINAGVYARANISFYSQTGIEWDLDLVNKTYEWVEDAIVVKPNNQSWQSVIGEGFLEAGI 194

Query: 61  GPDNGFDLRHIVGTK-------------------------------------------TG 120
            PDNGF L H  GT+                                           T 
Sbjct: 195 LPDNGFSLDHEAGTRLTGSTFDNNGTRHAADELLNKGDPNNLLVAVQASVEKILFSSNTS 254

Query: 121 GLSASGVSYSDSKGKLHRAFIRKKGEIILSAGAIRSPQLLILI----------------- 180
            LSA GV Y+DS G  H+AF+R  GE+I+SAG I +PQLL+L                  
Sbjct: 255 NLSAIGVIYTDSDGNSHQAFVRGNGEVIVSAGTIGTPQLLLLSGVGPESYLSSLNITVVQ 314

Query: 181 --------------------------ASSAQVVGTLDNNIHLQLFASPLPFFPLPSYSLL 240
                                     AS   V+G   +  + Q+  S LP F  P +SL 
Sbjct: 315 PNPYVGQFLYNNPRNFINNFPPNPIEASVVTVLGIRSD--YYQVSLSSLP-FSTPPFSLF 374

Query: 241 PSQSTSITPS-LAIFVGKFSNVHSKGWLHLNSSTDVKESPIVRFNYYSHHHDLTRCVRGV 300
           P+ S  +  S  A  V +     S G + LNSS+DV+ +P ++FNYYS+  DL  CV G+
Sbjct: 375 PTTSYPLPNSTFAHIVSQVPGPLSHGSVTLNSSSDVRIAPNIKFNYYSNSTDLANCVSGM 434

Query: 301 RKVGDLLKTQTMEKIKTEDLEGNKGFEFMELPMPENLWNDSLVEEYCRNTVATYWHYHGG 324
           +K+GDLL+T+ +E  K  D+ G  GF ++ +P+PEN  +D+  E +C + VA+YWHYHGG
Sbjct: 435 KKLGDLLRTKALEPYKARDVLGIDGFNYLGVPLPENQTDDASFETFCLDNVASYWHYHGG 494

[Close]

BLAST of Lsi11G007430 vs. ExPASy TrEMBL
Match: A0A1S3BTE4 ((R)-mandelonitrile lyase 3-like OS=Cucumis melo OX=3656 GN=LOC103493064 PE=3 SV=1)

HSP 1 Score: 530.4 bits (1365), Expect = 5.2e-147
Identity = 277/406 (68.23%), Postives = 297/406 (73.15%), Query Frame = 0


Query: 1   MVNAGFYSRGHQEFFERAGVDWDMEMVEKAYEWVEETVVSRPSLSGWQFAFRSALLEGGV 60
           MVNAGFYSRGH+EFFE AGVDW+ME+VEKAYEWVEET+VSRP+LS WQ AFR+ LLEGGV
Sbjct: 129 MVNAGFYSRGHREFFETAGVDWEMELVEKAYEWVEETLVSRPNLSAWQVAFRNTLLEGGV 188

Query: 61  GPDNGFDLRHIVGTKTG---------------------------------------GLSA 120
            PDNGFDLRH++GTKTG                                       GLSA
Sbjct: 189 VPDNGFDLRHLMGTKTGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRIIFNGLSA 248

Query: 121 SGVSYSDSKGKLHRAFIRKKGEIILSAGAIRSPQLLI----------------------- 180
            GV YSDSKGKLH A I KKGEI+LSAGAI SPQLL+                       
Sbjct: 249 VGVLYSDSKGKLHTALIHKKGEILLSAGAIGSPQLLLLSGIGPKSYLSSLKLHVVHHQPH 308

Query: 181 --------------------LIASSAQVVGTLDNNIHLQLFASPLPFFPLPSYSLLPSQS 240
                               L+ASSAQVVGTL+ NIHLQ  ASPLPF+PLPSY LLP QS
Sbjct: 309 VGQFMTDNPRFSRSIVFPFQLLASSAQVVGTLEKNIHLQSLASPLPFYPLPSYGLLPPQS 368

Query: 241 TSITPSLAIFVGKFSNVHSKGWLHL-NSSTDVKESPIVRFNYYSHHHDLTRCVRGVRKVG 300
           TSITPSL +FVGKFSNV SKGWLHL NSSTD KESPIVRFNYYS H DL+RCVRGVRKV 
Sbjct: 369 TSITPSLVVFVGKFSNVSSKGWLHLNNSSTDSKESPIVRFNYYSQHDDLSRCVRGVRKVE 428

Query: 301 DLLKTQTMEKIKTEDLEGNKGFEFMELPMPENLWNDSLVEEYCRNTVATYWHYHGGCLVG 324
           DLLKTQTME+ KT+DLEGNKGF+FM LPMPENLWNDS VEEYC+NTVATYWHYHGGCLVG
Sbjct: 429 DLLKTQTMERFKTQDLEGNKGFQFMGLPMPENLWNDSSVEEYCKNTVATYWHYHGGCLVG 488

[Close]

BLAST of Lsi11G007430 vs. ExPASy TrEMBL
Match: A0A0A0LMB8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G055040 PE=3 SV=1)

HSP 1 Score: 525.4 bits (1352), Expect = 1.7e-145
Identity = 276/406 (67.98%), Postives = 294/406 (72.41%), Query Frame = 0


Query: 1   MVNAGFYSRGHQEFFERAGVDWDMEMVEKAYEWVEETVVSRPSLSGWQFAFRSALLEGGV 60
           MVNAGFYSRGHQEFFE AGVDWDME+VEKAYEWVEET+VSRP+L+ WQ AFR+ LLEGGV
Sbjct: 128 MVNAGFYSRGHQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLTAWQAAFRNTLLEGGV 187

Query: 61  GPDNGFDLRHIVGTKTG---------------------------------------GLSA 120
            PDNGFDLRH+VGTK G                                       GLSA
Sbjct: 188 VPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRIIFKGLSA 247

Query: 121 SGVSYSDSKGKLHRAFIRKKGEIILSAGAIRSPQLLI----------------------- 180
            GV YSDSKGKLH A I KKGEI LSAGAI SPQLL+                       
Sbjct: 248 VGVLYSDSKGKLHTALIHKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLHLVHHQPH 307

Query: 181 --------------------LIASSAQVVGTLDNNIHLQLFASPLPFFPLPSYSLLPSQS 240
                               L+AS+AQVVGTL+ NIHLQ  ASPLPFFPLPSY LLP QS
Sbjct: 308 VGQYMTDNPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQSLASPLPFFPLPSYGLLPPQS 367

Query: 241 TSITPSLAIFVGKFSNVHSKGWLHL-NSSTDVKESPIVRFNYYSHHHDLTRCVRGVRKVG 300
           TSIT SL I VGKFSNV SKGWLHL NSSTD KE+PIVRFNYYS H D++RCV GVRKV 
Sbjct: 368 TSITSSLVIIVGKFSNVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGDISRCVSGVRKVE 427

Query: 301 DLLKTQTMEKIKTEDLEGNKGFEFMELPMPENLWNDSLVEEYCRNTVATYWHYHGGCLVG 324
           DLLKTQTME+IKT+DLEGNKGF+FMELPMPENLWNDS VEEYC+NTVATYWHYHGGCLVG
Sbjct: 428 DLLKTQTMERIKTQDLEGNKGFQFMELPMPENLWNDSSVEEYCKNTVATYWHYHGGCLVG 487

[Close]

BLAST of Lsi11G007430 vs. ExPASy TrEMBL
Match: A0A5D3CI61 ((R)-mandelonitrile lyase 3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold231G00870 PE=3 SV=1)

HSP 1 Score: 521.9 bits (1343), Expect = 1.9e-144
Identity = 273/400 (68.25%), Postives = 292/400 (73.00%), Query Frame = 0


Query: 1   MVNAGFYSRGHQEFFERAGVDWDMEMVEKAYEWVEETVVSRPSLSGWQFAFRSALLEGGV 60
           MVNAGFYSRGH+EFFE AGVDW+ME+VEKAYEWVEET+VSRP+LS WQ AFR+ LLEGGV
Sbjct: 97  MVNAGFYSRGHREFFETAGVDWEMELVEKAYEWVEETLVSRPNLSAWQVAFRNTLLEGGV 156

Query: 61  GPDNGFDLRHIVGTKTG---------------------------------------GLSA 120
            PDNGFDLRH++GTKTG                                       GLSA
Sbjct: 157 VPDNGFDLRHLMGTKTGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRIIFNGLSA 216

Query: 121 SGVSYSDSKGKLHRAFIRKKGEIILSAGAIRSPQLLI----------------------- 180
            GV YSDSKGKLH A I KKGEI+LSAGAI SPQLL+                       
Sbjct: 217 VGVLYSDSKGKLHTALIHKKGEILLSAGAIGSPQLLLLSGIGPKSYLSSLKLHVVHHQPH 276

Query: 181 --------------------LIASSAQVVGTLDNNIHLQLFASPLPFFPLPSYSLLPSQS 240
                               L+ASSAQVVGTL+ NIHLQ  ASPLPF+PLPSY LLP QS
Sbjct: 277 VGQFMTDNPRFSRSIVFPFQLLASSAQVVGTLEKNIHLQSLASPLPFYPLPSYGLLPPQS 336

Query: 241 TSITPSLAIFVGKFSNVHSKGWLHL-NSSTDVKESPIVRFNYYSHHHDLTRCVRGVRKVG 300
           TSITPSL +FVGKFSNV SKGWLHL NSSTD KESPIVRFNYYS H DL+RCVRGVRKV 
Sbjct: 337 TSITPSLVVFVGKFSNVSSKGWLHLNNSSTDSKESPIVRFNYYSQHDDLSRCVRGVRKVE 396

Query: 301 DLLKTQTMEKIKTEDLEGNKGFEFMELPMPENLWNDSLVEEYCRNTVATYWHYHGGCLVG 318
           DLLKTQTME+ KT+DLEGNKGF+FM LPMPENLWNDS VEEYC+NTVATYWHYHGGCLVG
Sbjct: 397 DLLKTQTMERFKTQDLEGNKGFQFMGLPMPENLWNDSSVEEYCKNTVATYWHYHGGCLVG 456

[Close]

BLAST of Lsi11G007430 vs. ExPASy TrEMBL
Match: A0A5D3CL00 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold231G00890 PE=3 SV=1)

HSP 1 Score: 516.9 bits (1330), Expect = 6.0e-143
Identity = 275/405 (67.90%), Postives = 296/405 (73.09%), Query Frame = 0


Query: 1   MVNAGFYSRGHQEFFERAGVDWDMEMVEKAYEWVEETVVSRPSLSGWQFAFRSALLEGGV 60
           M+NAGFYSRGH+EFFE AGVDWDME+VEKAYEWVEETVVS+PSLS WQ AFRSALLEGGV
Sbjct: 128 MINAGFYSRGHREFFETAGVDWDMELVEKAYEWVEETVVSKPSLSPWQAAFRSALLEGGV 187

Query: 61  GPDNGFDLRHIVGTKTG---------------------------------------GLSA 120
           G DNGFDL+H+VGTKTG                                       GLSA
Sbjct: 188 GHDNGFDLKHLVGTKTGGSIFDNKGNRHGAVELLNKGEPENFKVATQATVQRIIFTGLSA 247

Query: 121 SGVSYSDSKGKLHRAFIRKKGEIILSAGAIRSPQLLIL---------------------- 180
           SGVSYSDSKGKLH AFIRKKGEIILSAGAI SPQLL+L                      
Sbjct: 248 SGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSQLKSLKLPVVLDQPH 307

Query: 181 ---------------------IASSAQVVGTLDNNIHLQLFASPLPFFPLPSYSLLPSQS 240
                                +ASSAQVVGTLDNNIHLQ FASPLPFF  PS+SLLP QS
Sbjct: 308 VGEFMSDNPRFTPTIVLPFQVVASSAQVVGTLDNNIHLQAFASPLPFFAPPSFSLLPPQS 367

Query: 241 TSITPSLAIFVGKFSNVHSKGWLHLNSSTDVKESPIVRFNYYSHHHDLTRCVRGVRKVGD 300
           TSI PSLAIFVGKFS+V+S+G L LNSS DVKESPIVRFNYYSH  DL RCVRGVRKVGD
Sbjct: 368 TSIVPSLAIFVGKFSDVYSEGSLRLNSSIDVKESPIVRFNYYSHPDDLARCVRGVRKVGD 427

Query: 301 LLKTQTMEKIKTEDLEGNKGFEFMELPMPENLWNDSLVEEYCRNTVATYWHYHGGCLVGK 324
           LL+T TMEKIKT+DLEGNK F+F+ L +PENL NDS VEEYC+ TV TYWHYHGGCLVGK
Sbjct: 428 LLRTPTMEKIKTQDLEGNKRFQFLGLSLPENLLNDSAVEEYCQKTVTTYWHYHGGCLVGK 487

[Close]

BLAST of Lsi11G007430 vs. ExPASy TrEMBL
Match: A0A1S3BSP4 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493073 PE=3 SV=1)

HSP 1 Score: 516.9 bits (1330), Expect = 6.0e-143
Identity = 275/405 (67.90%), Postives = 296/405 (73.09%), Query Frame = 0


Query: 1   MVNAGFYSRGHQEFFERAGVDWDMEMVEKAYEWVEETVVSRPSLSGWQFAFRSALLEGGV 60
           M+NAGFYSRGH+EFFE AGVDWDME+VEKAYEWVEETVVS+PSLS WQ AFRSALLEGGV
Sbjct: 128 MINAGFYSRGHREFFETAGVDWDMELVEKAYEWVEETVVSKPSLSPWQAAFRSALLEGGV 187

Query: 61  GPDNGFDLRHIVGTKTG---------------------------------------GLSA 120
           G DNGFDL+H+VGTKTG                                       GLSA
Sbjct: 188 GHDNGFDLKHLVGTKTGGSIFDNKGNRHGAVELLNKGEPENFKVATQATVQRIIFTGLSA 247

Query: 121 SGVSYSDSKGKLHRAFIRKKGEIILSAGAIRSPQLLIL---------------------- 180
           SGVSYSDSKGKLH AFIRKKGEIILSAGAI SPQLL+L                      
Sbjct: 248 SGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSQLKSLKLPVVLDQPH 307

Query: 181 ---------------------IASSAQVVGTLDNNIHLQLFASPLPFFPLPSYSLLPSQS 240
                                +ASSAQVVGTLDNNIHLQ FASPLPFF  PS+SLLP QS
Sbjct: 308 VGEFMSDNPRFTPTIVLPFQVVASSAQVVGTLDNNIHLQAFASPLPFFAPPSFSLLPPQS 367

Query: 241 TSITPSLAIFVGKFSNVHSKGWLHLNSSTDVKESPIVRFNYYSHHHDLTRCVRGVRKVGD 300
           TSI PSLAIFVGKFS+V+S+G L LNSS DVKESPIVRFNYYSH  DL RCVRGVRKVGD
Sbjct: 368 TSIVPSLAIFVGKFSDVYSEGSLRLNSSIDVKESPIVRFNYYSHPDDLARCVRGVRKVGD 427

Query: 301 LLKTQTMEKIKTEDLEGNKGFEFMELPMPENLWNDSLVEEYCRNTVATYWHYHGGCLVGK 324
           LL+T TMEKIKT+DLEGNK F+F+ L +PENL NDS VEEYC+ TV TYWHYHGGCLVGK
Sbjct: 428 LLRTPTMEKIKTQDLEGNKRFQFLGLSLPENLLNDSAVEEYCQKTVTTYWHYHGGCLVGK 487

[Close]

BLAST of Lsi11G007430 vs. NCBI nr
Match: XP_008451913.1 (PREDICTED: (R)-mandelonitrile lyase 3-like [Cucumis melo])

HSP 1 Score: 530.4 bits (1365), Expect = 1.1e-146
Identity = 277/406 (68.23%), Postives = 297/406 (73.15%), Query Frame = 0


Query: 1   MVNAGFYSRGHQEFFERAGVDWDMEMVEKAYEWVEETVVSRPSLSGWQFAFRSALLEGGV 60
           MVNAGFYSRGH+EFFE AGVDW+ME+VEKAYEWVEET+VSRP+LS WQ AFR+ LLEGGV
Sbjct: 129 MVNAGFYSRGHREFFETAGVDWEMELVEKAYEWVEETLVSRPNLSAWQVAFRNTLLEGGV 188

Query: 61  GPDNGFDLRHIVGTKTG---------------------------------------GLSA 120
            PDNGFDLRH++GTKTG                                       GLSA
Sbjct: 189 VPDNGFDLRHLMGTKTGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRIIFNGLSA 248

Query: 121 SGVSYSDSKGKLHRAFIRKKGEIILSAGAIRSPQLLI----------------------- 180
            GV YSDSKGKLH A I KKGEI+LSAGAI SPQLL+                       
Sbjct: 249 VGVLYSDSKGKLHTALIHKKGEILLSAGAIGSPQLLLLSGIGPKSYLSSLKLHVVHHQPH 308

Query: 181 --------------------LIASSAQVVGTLDNNIHLQLFASPLPFFPLPSYSLLPSQS 240
                               L+ASSAQVVGTL+ NIHLQ  ASPLPF+PLPSY LLP QS
Sbjct: 309 VGQFMTDNPRFSRSIVFPFQLLASSAQVVGTLEKNIHLQSLASPLPFYPLPSYGLLPPQS 368

Query: 241 TSITPSLAIFVGKFSNVHSKGWLHL-NSSTDVKESPIVRFNYYSHHHDLTRCVRGVRKVG 300
           TSITPSL +FVGKFSNV SKGWLHL NSSTD KESPIVRFNYYS H DL+RCVRGVRKV 
Sbjct: 369 TSITPSLVVFVGKFSNVSSKGWLHLNNSSTDSKESPIVRFNYYSQHDDLSRCVRGVRKVE 428

Query: 301 DLLKTQTMEKIKTEDLEGNKGFEFMELPMPENLWNDSLVEEYCRNTVATYWHYHGGCLVG 324
           DLLKTQTME+ KT+DLEGNKGF+FM LPMPENLWNDS VEEYC+NTVATYWHYHGGCLVG
Sbjct: 429 DLLKTQTMERFKTQDLEGNKGFQFMGLPMPENLWNDSSVEEYCKNTVATYWHYHGGCLVG 488

[Close]

BLAST of Lsi11G007430 vs. NCBI nr
Match: XP_011648923.1 ((R)-mandelonitrile lyase 3 [Cucumis sativus] >KGN61116.1 hypothetical protein Csa_021230 [Cucumis sativus])

HSP 1 Score: 525.4 bits (1352), Expect = 3.5e-145
Identity = 276/406 (67.98%), Postives = 294/406 (72.41%), Query Frame = 0


Query: 1   MVNAGFYSRGHQEFFERAGVDWDMEMVEKAYEWVEETVVSRPSLSGWQFAFRSALLEGGV 60
           MVNAGFYSRGHQEFFE AGVDWDME+VEKAYEWVEET+VSRP+L+ WQ AFR+ LLEGGV
Sbjct: 128 MVNAGFYSRGHQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLTAWQAAFRNTLLEGGV 187

Query: 61  GPDNGFDLRHIVGTKTG---------------------------------------GLSA 120
            PDNGFDLRH+VGTK G                                       GLSA
Sbjct: 188 VPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRIIFKGLSA 247

Query: 121 SGVSYSDSKGKLHRAFIRKKGEIILSAGAIRSPQLLI----------------------- 180
            GV YSDSKGKLH A I KKGEI LSAGAI SPQLL+                       
Sbjct: 248 VGVLYSDSKGKLHTALIHKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLHLVHHQPH 307

Query: 181 --------------------LIASSAQVVGTLDNNIHLQLFASPLPFFPLPSYSLLPSQS 240
                               L+AS+AQVVGTL+ NIHLQ  ASPLPFFPLPSY LLP QS
Sbjct: 308 VGQYMTDNPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQSLASPLPFFPLPSYGLLPPQS 367

Query: 241 TSITPSLAIFVGKFSNVHSKGWLHL-NSSTDVKESPIVRFNYYSHHHDLTRCVRGVRKVG 300
           TSIT SL I VGKFSNV SKGWLHL NSSTD KE+PIVRFNYYS H D++RCV GVRKV 
Sbjct: 368 TSITSSLVIIVGKFSNVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGDISRCVSGVRKVE 427

Query: 301 DLLKTQTMEKIKTEDLEGNKGFEFMELPMPENLWNDSLVEEYCRNTVATYWHYHGGCLVG 324
           DLLKTQTME+IKT+DLEGNKGF+FMELPMPENLWNDS VEEYC+NTVATYWHYHGGCLVG
Sbjct: 428 DLLKTQTMERIKTQDLEGNKGFQFMELPMPENLWNDSSVEEYCKNTVATYWHYHGGCLVG 487

[Close]

BLAST of Lsi11G007430 vs. NCBI nr
Match: KAA0067297.1 ((R)-mandelonitrile lyase 3-like [Cucumis melo var. makuwa] >TYK10908.1 (R)-mandelonitrile lyase 3-like [Cucumis melo var. makuwa])

HSP 1 Score: 521.9 bits (1343), Expect = 3.8e-144
Identity = 273/400 (68.25%), Postives = 292/400 (73.00%), Query Frame = 0


Query: 1   MVNAGFYSRGHQEFFERAGVDWDMEMVEKAYEWVEETVVSRPSLSGWQFAFRSALLEGGV 60
           MVNAGFYSRGH+EFFE AGVDW+ME+VEKAYEWVEET+VSRP+LS WQ AFR+ LLEGGV
Sbjct: 97  MVNAGFYSRGHREFFETAGVDWEMELVEKAYEWVEETLVSRPNLSAWQVAFRNTLLEGGV 156

Query: 61  GPDNGFDLRHIVGTKTG---------------------------------------GLSA 120
            PDNGFDLRH++GTKTG                                       GLSA
Sbjct: 157 VPDNGFDLRHLMGTKTGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRIIFNGLSA 216

Query: 121 SGVSYSDSKGKLHRAFIRKKGEIILSAGAIRSPQLLI----------------------- 180
            GV YSDSKGKLH A I KKGEI+LSAGAI SPQLL+                       
Sbjct: 217 VGVLYSDSKGKLHTALIHKKGEILLSAGAIGSPQLLLLSGIGPKSYLSSLKLHVVHHQPH 276

Query: 181 --------------------LIASSAQVVGTLDNNIHLQLFASPLPFFPLPSYSLLPSQS 240
                               L+ASSAQVVGTL+ NIHLQ  ASPLPF+PLPSY LLP QS
Sbjct: 277 VGQFMTDNPRFSRSIVFPFQLLASSAQVVGTLEKNIHLQSLASPLPFYPLPSYGLLPPQS 336

Query: 241 TSITPSLAIFVGKFSNVHSKGWLHL-NSSTDVKESPIVRFNYYSHHHDLTRCVRGVRKVG 300
           TSITPSL +FVGKFSNV SKGWLHL NSSTD KESPIVRFNYYS H DL+RCVRGVRKV 
Sbjct: 337 TSITPSLVVFVGKFSNVSSKGWLHLNNSSTDSKESPIVRFNYYSQHDDLSRCVRGVRKVE 396

Query: 301 DLLKTQTMEKIKTEDLEGNKGFEFMELPMPENLWNDSLVEEYCRNTVATYWHYHGGCLVG 318
           DLLKTQTME+ KT+DLEGNKGF+FM LPMPENLWNDS VEEYC+NTVATYWHYHGGCLVG
Sbjct: 397 DLLKTQTMERFKTQDLEGNKGFQFMGLPMPENLWNDSSVEEYCKNTVATYWHYHGGCLVG 456

[Close]

BLAST of Lsi11G007430 vs. NCBI nr
Match: XP_038889699.1 ((R)-mandelonitrile lyase 1-like [Benincasa hispida])

HSP 1 Score: 518.1 bits (1333), Expect = 5.5e-143
Identity = 273/405 (67.41%), Postives = 295/405 (72.84%), Query Frame = 0


Query: 1   MVNAGFYSRGHQEFFERAGVDWDMEMVEKAYEWVEETVVSRPSLSGWQFAFRSALLEGGV 60
           M+NAGFYSRGH+EFFE AGVDWDME+VEKAY+WVE+TVVSRPSLS WQ AFR ALLE GV
Sbjct: 131 MINAGFYSRGHREFFETAGVDWDMELVEKAYQWVEQTVVSRPSLSAWQSAFRGALLEAGV 190

Query: 61  GPDNGFDLRHIVGTKTG---------------------------------------GLSA 120
           GPDNGFDL+H+VGTKTG                                       GLSA
Sbjct: 191 GPDNGFDLKHLVGTKTGGSIFDNKGNRHGAVELLNKAEPQNLKVGTQAIVERIIFTGLSA 250

Query: 121 SGVSYSDSKGKLHRAFIRKKGEIILSAGAIRSPQLLI----------------------- 180
           SGVSYSD KGKLH AFIR+KGEIILSAGAI SPQLL+                       
Sbjct: 251 SGVSYSDPKGKLHTAFIREKGEIILSAGAIGSPQLLLLSGVGPKYHLSSLKLPVVLHQPH 310

Query: 181 --------------------LIASSAQVVGTLDNNIHLQLFASPLPFFPLPSYSLLPSQS 240
                               L+ASSAQVVGTL NNIHLQ FASPLPFF  PS+SLLP QS
Sbjct: 311 VGEFMSDNPRFSPTIVLPFQLVASSAQVVGTLANNIHLQSFASPLPFFAPPSFSLLPPQS 370

Query: 241 TSITPSLAIFVGKFSNVHSKGWLHLNSSTDVKESPIVRFNYYSHHHDLTRCVRGVRKVGD 300
           TSI PSLAIFVGKFS+V S+G L LNSS DVKESPIVRFNYYS+H DL RCVRGVRKVGD
Sbjct: 371 TSIIPSLAIFVGKFSDVLSEGSLRLNSSIDVKESPIVRFNYYSNHDDLARCVRGVRKVGD 430

Query: 301 LLKTQTMEKIKTEDLEGNKGFEFMELPMPENLWNDSLVEEYCRNTVATYWHYHGGCLVGK 324
           LLKTQTMEKIK +DLEGNKGF+F+ LP+PENLWNDS VEEYC+ TV TYWHYHGGCLVGK
Sbjct: 431 LLKTQTMEKIKNQDLEGNKGFQFLGLPLPENLWNDSAVEEYCQKTVTTYWHYHGGCLVGK 490

[Close]

BLAST of Lsi11G007430 vs. NCBI nr
Match: XP_008451922.1 (PREDICTED: (R)-mandelonitrile lyase 1-like [Cucumis melo] >KAA0067298.1 (R)-mandelonitrile lyase 1-like [Cucumis melo var. makuwa] >TYK10909.1 (R)-mandelonitrile lyase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 516.9 bits (1330), Expect = 1.2e-142
Identity = 275/405 (67.90%), Postives = 296/405 (73.09%), Query Frame = 0


Query: 1   MVNAGFYSRGHQEFFERAGVDWDMEMVEKAYEWVEETVVSRPSLSGWQFAFRSALLEGGV 60
           M+NAGFYSRGH+EFFE AGVDWDME+VEKAYEWVEETVVS+PSLS WQ AFRSALLEGGV
Sbjct: 128 MINAGFYSRGHREFFETAGVDWDMELVEKAYEWVEETVVSKPSLSPWQAAFRSALLEGGV 187

Query: 61  GPDNGFDLRHIVGTKTG---------------------------------------GLSA 120
           G DNGFDL+H+VGTKTG                                       GLSA
Sbjct: 188 GHDNGFDLKHLVGTKTGGSIFDNKGNRHGAVELLNKGEPENFKVATQATVQRIIFTGLSA 247

Query: 121 SGVSYSDSKGKLHRAFIRKKGEIILSAGAIRSPQLLIL---------------------- 180
           SGVSYSDSKGKLH AFIRKKGEIILSAGAI SPQLL+L                      
Sbjct: 248 SGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSQLKSLKLPVVLDQPH 307

Query: 181 ---------------------IASSAQVVGTLDNNIHLQLFASPLPFFPLPSYSLLPSQS 240
                                +ASSAQVVGTLDNNIHLQ FASPLPFF  PS+SLLP QS
Sbjct: 308 VGEFMSDNPRFTPTIVLPFQVVASSAQVVGTLDNNIHLQAFASPLPFFAPPSFSLLPPQS 367

Query: 241 TSITPSLAIFVGKFSNVHSKGWLHLNSSTDVKESPIVRFNYYSHHHDLTRCVRGVRKVGD 300
           TSI PSLAIFVGKFS+V+S+G L LNSS DVKESPIVRFNYYSH  DL RCVRGVRKVGD
Sbjct: 368 TSIVPSLAIFVGKFSDVYSEGSLRLNSSIDVKESPIVRFNYYSHPDDLARCVRGVRKVGD 427

Query: 301 LLKTQTMEKIKTEDLEGNKGFEFMELPMPENLWNDSLVEEYCRNTVATYWHYHGGCLVGK 324
           LL+T TMEKIKT+DLEGNK F+F+ L +PENL NDS VEEYC+ TV TYWHYHGGCLVGK
Sbjct: 428 LLRTPTMEKIKTQDLEGNKRFQFLGLSLPENLLNDSAVEEYCQKTVTTYWHYHGGCLVGK 487

[Close]

BLAST of Lsi11G007430 vs. TAIR 10
Match: AT1G73050.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 280.0 bits (715), Expect = 2.4e-75
Identity = 166/413 (40.19%), Postives = 217/413 (52.54%), Query Frame = 0


Query: 2   VNAGFYSRGHQEFFERAGVDWDMEMVEKAYEWVEETVVSRPSLSGWQFAFRSALLEGGVG 61
           +NAGFYSR  ++FFE +G+ WD+  V ++YEWVE  +V RP L  WQ A R ALLE GV 
Sbjct: 136 INAGFYSRADKQFFENSGLVWDLSSVNQSYEWVERAIVFRPQLRTWQTAIRDALLEVGVH 195

Query: 62  PDNGFDLRHIVGTKTGG------------------------------------------- 121
           P NGF L H VGTK GG                                           
Sbjct: 196 PFNGFTLEHKVGTKIGGSTFDRTGRRHSSADLLRYARSSNIRVAVYATVERVLLASSPSV 255

Query: 122 ----LSASGVSYSDSKGKLHRAFIRKKGEIILSAGAIRSPQLLILIA------------- 181
               +SA GV Y D  G+ H A IR +GE+ILSAGA+ SPQLL L               
Sbjct: 256 SGSNVSAIGVVYRDQLGRFHHALIRDRGEVILSAGALGSPQLLFLSGIGPRSYLSTWGIP 315

Query: 182 ------------------------------SSAQVVGTLDNNIHLQLFASPLPF-FPLPS 241
                                         S  QVVG  ++   L+  ++ +PF  PL S
Sbjct: 316 VALDQPHVGDFVYDNPRNGISIVPPVPMENSLIQVVGVTEDGAFLEAASNVIPFASPLHS 375

Query: 242 YSLLPSQSTSITPSLAIFVGKFSNVHSKGWLHLNSSTDVKESPIVRFNYYSHHHDLTRCV 301
             +    S    P   I + K     S G L L +STDV+ +P+VRFNY+S   DL RCV
Sbjct: 376 VFIRAPASPLYVPVTTI-MEKILGPVSIGLLRL-ASTDVRINPVVRFNYFSDPQDLERCV 435

Query: 302 RGVRKVGDLLKTQTMEKIKTEDLEGNKGFEFMELPMPENLWNDSLVEEYCRNTVATYWHY 324
            G RK+G++L+++ M+     +  GN+ F F+  P+P +  ND ++ ++CR TV+T WHY
Sbjct: 436 NGTRKIGEILRSRAMQDFMIREWFGNRRFRFVGAPLPVDQSNDLVMADFCRRTVSTIWHY 495

[Close]

BLAST of Lsi11G007430 vs. TAIR 10
Match: AT3G56060.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 206.8 bits (525), Expect = 2.5e-53
Identity = 149/439 (33.94%), Postives = 205/439 (46.70%), Query Frame = 0


Query: 1   MVNAGFYSRGHQEFFERAGVDWDMEMVEKAYEWVEETVVSRPSLSGWQFAFRSALLEGGV 60
           ++NAGFYSR  ++F   AG  W+ + VE AYEWVE+ VV  P ++ WQ AFR  LLE GV
Sbjct: 126 VINAGFYSRAEEDFVAEAG--WERDEVEAAYEWVEKKVVFEPPVNKWQSAFRDGLLEAGV 185

Query: 61  GPDNGFDLRHIVGTKTGGL----------------------------------------- 120
            P NGF   HIVGTK GG                                          
Sbjct: 186 TPYNGFTYEHIVGTKFGGTIFDRDGHRHTAANLLEYANPNMIVVYLHASVHKILFTIKGN 245

Query: 121 ---SASGVSYSDSKGKLHRAFIRKK----GEIILSAGAIRSPQLLIL--IASSAQVV--- 180
               A GV + D+ G  ++A +  +     E+ILSAGAI SPQLL+L  +  +A +    
Sbjct: 246 QRPKAYGVIFLDANGVSYKAELATQDSTMSEVILSAGAIASPQLLMLSGVGPAAHLAAYR 305

Query: 181 -------------GTLDNNIHLQLFASPLP------------------------------ 240
                        G  DN ++     SP P                              
Sbjct: 306 VNPVIVDQPMVGQGMGDNPMNPVFIPSPEPVEVSLVQAVGITKFGSYIEGGSALSLSISL 365

Query: 241 ---FFP----LPSYSLLPSQSTS-----------ITPSLAIFVGKFSNVHSKGWLHLNSS 300
              FF     L   + LP+QS S           +T    + + K +   S+G L L  +
Sbjct: 366 TRSFFDGVLNLLKKTKLPTQSISKFFKSLDLTLNVTTKAGVIIQKVNGPLSRGHLELR-N 425

Query: 301 TDVKESPIVRFNYYSHHHDLTRCVRGVRKVGDLLKTQTMEKIKTEDLEGNKGFEFMELPM 321
           T+  ++P V FNY+    DL +CV G+  +  ++ ++   K K   L   +G   + L +
Sbjct: 426 TNPDDNPSVTFNYFKDPEDLNKCVEGLSTIIKVIDSKGYSKYK-YPLASARGLLNLILAL 485

[Close]

BLAST of Lsi11G007430 vs. TAIR 10
Match: AT5G51930.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 200.3 bits (508), Expect = 2.4e-51
Identity = 144/433 (33.26%), Postives = 198/433 (45.73%), Query Frame = 0


Query: 1   MVNAGFYSRGHQEFFERAGVDWDMEMVEKAYEWVEETVVSRPSLSGWQFAFRSALLEGGV 60
           ++N GFYSR   ++ E A  +W+ME VE AYEWVE+ +V  P +  WQ AF+  LLE G 
Sbjct: 153 VINGGFYSRAGNDYVEEA--EWEMEEVEAAYEWVEKKLVFEPQVIEWQKAFKDGLLEAGE 212

Query: 61  GPDNGFDLRHIVGTKTGG--------------------------------------LSAS 120
            PDNGF   HI GTK GG                                        A 
Sbjct: 213 SPDNGFTYDHIYGTKIGGTIFDRAGHRHTAANLLEYANPNRIVVYLHASVHKVLFTTEAY 272

Query: 121 GVSYSDSKGKLHRAFIRKK--GEIILSAGAIRSPQLLILIASSAQV-------------- 180
            V + D+ G  H+A +  K   E+ILSAGA+ SPQLL+L      V              
Sbjct: 273 EVLFEDANGVFHKANLANKATNEVILSAGALGSPQLLMLSGVGPAVHLEAHGVNPLVLDQ 332

Query: 181 ----VGTLDNNIHLQLFASPLP----------FFPLPSY--------------------- 240
                G  DN ++     SP P               SY                     
Sbjct: 333 PMVGQGMADNPMNFVAIPSPQPVELSLIQAVGITKFDSYIEGLSGLSLSFDITRRFFDGV 392

Query: 241 -SLL--PSQSTS---ITPSLAIFVGKF-----------------SNVHSKGWLHLNSSTD 300
            +LL   S +TS   +T S+A+ +  F                     SKG + L  +T+
Sbjct: 393 LNLLNETSHTTSRKILTQSIAVLLKSFDVKLEVRMNGGLIFQKVDGPASKGHMKLR-NTN 452

Query: 301 VKESPIVRFNYYSHHHDLTRCVRGVRKVGDLLKTQTMEKIKTEDLEGNKGFEFM-ELPM- 318
            +++P V FNYY    DL +CV+G+  +  ++ ++   K K   +   +    M  LP+ 
Sbjct: 453 PRDNPSVTFNYYQEPEDLNKCVKGLNTIIRMINSKAFSKYKYPGVTARELLNLMLALPIN 512

[Close]

BLAST of Lsi11G007430 vs. TAIR 10
Match: AT1G12570.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 199.9 bits (507), Expect = 3.1e-51
Identity = 146/444 (32.88%), Postives = 204/444 (45.95%), Query Frame = 0


Query: 2   VNAGFYSRGHQEFFERAGVDWDMEMVEKAYEWVEETVVSRPSLSGWQFAFRSALLEGGVG 61
           +NAGFY+R   ++    G  WD  +  ++Y+WVE  V  +P +  WQ A R  LLE G+ 
Sbjct: 127 LNAGFYTRAGTKYVRNMG--WDGALANESYQWVEAKVAFQPPMGRWQTAVRDGLLEAGIV 186

Query: 62  PDNGFDLRHIVGTKTGGL------------------------------------------ 121
           P+NGF   HI GTK GG                                           
Sbjct: 187 PNNGFTYDHINGTKFGGTIFDRNGNRHTAADLLEYADPKGITVLLHATVHRILFRTRGTT 246

Query: 122 --SASGVSYSDSKGKLHRAFIRK--KGEIILSAGAIRSPQLLIL--IASSAQV------- 181
              A+GV Y D  G+ HRA++++    EIILSAG + SPQLL+L  +  SAQ+       
Sbjct: 247 KPIANGVVYRDRTGQAHRAYLKEGALSEIILSAGTLGSPQLLMLSGVGPSAQLQAQNITV 306

Query: 182 --------VGTLDNNIHLQLFASPLP---------------------------------- 241
                    G  DN ++     SP+P                                  
Sbjct: 307 VMDQPHVGQGMYDNPMNAVFVPSPVPVEVSLIEVVGITGEGTYVEAAGGENFGGGGGGSS 366

Query: 242 -------FFPL--PSYSLLPSQSTSITPSLAIFVGKFSNVHSKGWL---HLNSST-DVKE 301
                  ++ +  P  +LL S S +   S   F G F      G L   HL   T + K+
Sbjct: 367 GSSSTRDYYAMFSPRATLLESNSMTKLSSAQPFQGGFLLEKVMGPLSTGHLELKTRNPKD 426

Query: 302 SPIVRFNYYSHHHDLTRCVRGVRKVGDLLKTQTMEKIKTEDLEGNKGFEFM--------- 324
           +PIV FNY+ H  DL RCVRG++ +  +++++   + K  D+     FE++         
Sbjct: 427 NPIVTFNYFQHPDDLKRCVRGIQTIERVVQSKAFSRYKYADV----SFEYLLNLTASTPV 486

[Close]

BLAST of Lsi11G007430 vs. TAIR 10
Match: AT5G51950.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 197.6 bits (501), Expect = 1.5e-50
Identity = 147/448 (32.81%), Postives = 204/448 (45.54%), Query Frame = 0


Query: 1   MVNAGFYSRGHQEFFERAGVDWDMEMVEKAYEWVEETVVSRPSLSGWQFAFRSALLEGGV 60
           ++NAGFY+R   E+ +    +W  + VE AYEWVE+ V  +P + GWQ AF+  LLE G 
Sbjct: 126 VLNAGFYTRAGDEYVKE--TEWKTDEVEAAYEWVEKKVAFQPPVLGWQTAFKDGLLEAGE 185

Query: 61  GPDNGFDLRHIVGTKTGGL----------------------------------------- 120
            P NGF   HI GTK GG                                          
Sbjct: 186 FPYNGFTYDHIYGTKIGGTIFDRAGHRHTAADLLEYANPGNIVVYLHASVHKILFTTKGR 245

Query: 121 ---SASGVSYSDSKGKLHRAFIRKK--GEIILSAGAIRSPQLLIL--IASSAQVV----- 180
               A GV + D+ G LH+A + K    E+ILSAGAI SPQLL+L  I  +A +      
Sbjct: 246 PRPKAYGVIFQDANGVLHKAELEKNSMNEVILSAGAIGSPQLLMLSGIGPAAHLAAHGIK 305

Query: 181 -----------GTLDNNIHLQLFASPLP-------------------------------- 240
                      G  DN ++     SP P                                
Sbjct: 306 PLVLDHPMVGQGMGDNPMNAIFIPSPTPVEVSLIQVVGITKFESYIEGASGVIFSYSWTR 365

Query: 241 --FFPLPSY--SLLPSQSTSIT-PSLA----------------------IFVGKFSNVHS 300
             F  + +Y   +  S++TS T P+L+                      + + K +   S
Sbjct: 366 RFFDGVLNYLNEMQTSRTTSTTSPTLSTQSITDFFNPINPLLNATTRAGLILQKIAGPIS 425

Query: 301 KGWLHLNSSTDVKESPIVRFNYYSHHHDLTRCVRGVRKVGDLLKTQTMEKIKTEDLEGNK 321
           +G L L  +T+  ++P VRFNYY    DL  CV G+  +  ++ ++   K K  D   + 
Sbjct: 426 RGHLELR-NTNPDDNPSVRFNYYQEPEDLQICVEGINTIIKVINSKAFSKFKYPDATIHG 485

The following BLAST results are available for this feature:

BLAST of Lsi11G007430 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SSM2	3.3e-74	40.19	(R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 ...	[more]
P52707	1.4e-72	37.56	(R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1	[more]
P52706	4.1e-72	39.31	(R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1	[more]
Q945K2	1.7e-70	38.33	(R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1	[more]
O24243	1.4e-69	36.83	(R)-mandelonitrile lyase 1 OS=Prunus dulcis OX=3755 GN=MDL1 PE=1 SV=1	[more]

BLAST of Lsi11G007430 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BTE4	5.2e-147	68.23	(R)-mandelonitrile lyase 3-like OS=Cucumis melo OX=3656 GN=LOC103493064 PE=3 SV=...	[more]
A0A0A0LMB8	1.7e-145	67.98	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G055040 PE=3 SV=1	[more]
A0A5D3CI61	1.9e-144	68.25	(R)-mandelonitrile lyase 3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]
A0A5D3CL00	6.0e-143	67.90	(R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]
A0A1S3BSP4	6.0e-143	67.90	(R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493073 PE=3 SV=...	[more]

BLAST of Lsi11G007430 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008451913.1	1.1e-146	68.23	PREDICTED: (R)-mandelonitrile lyase 3-like [Cucumis melo]	[more]
XP_011648923.1	3.5e-145	67.98	(R)-mandelonitrile lyase 3 [Cucumis sativus] >KGN61116.1 hypothetical protein Cs...	[more]
KAA0067297.1	3.8e-144	68.25	(R)-mandelonitrile lyase 3-like [Cucumis melo var. makuwa] >TYK10908.1 (R)-mande...	[more]
XP_038889699.1	5.5e-143	67.41	(R)-mandelonitrile lyase 1-like [Benincasa hispida]	[more]
XP_008451922.1	1.2e-142	67.90	PREDICTED: (R)-mandelonitrile lyase 1-like [Cucumis melo] >KAA0067298.1 (R)-mand...	[more]

BLAST of Lsi11G007430 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G73050.1	2.4e-75	40.19	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT3G56060.1	2.5e-53	33.94	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT5G51930.1	2.4e-51	33.26	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT1G12570.1	3.1e-51	32.88	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT5G51950.1	1.5e-50	32.81	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036188	FAD/NAD(P)-binding domain superfamily	GENE3D	3.50.50.60		coord: 1..39 e-value: 1.3E-18 score: 69.2
IPR036188	FAD/NAD(P)-binding domain superfamily	GENE3D	3.50.50.60		coord: 177..316 e-value: 2.3E-55 score: 190.5
IPR036188	FAD/NAD(P)-binding domain superfamily	GENE3D	3.50.50.60		coord: 80..119 e-value: 6.3E-10 score: 40.6
IPR036188	FAD/NAD(P)-binding domain superfamily	SUPERFAMILY	51905	FAD/NAD(P)-binding domain	coord: 2..320
None	No IPR available	GENE3D	3.30.410.40		coord: 125..266 e-value: 2.3E-55 score: 190.5
None	No IPR available	GENE3D	3.30.410.40		coord: 40..79 e-value: 1.3E-18 score: 69.2
None	No IPR available	PANTHER	PTHR45968:SF23	(R)-MANDELONITRILE LYASE 4-RELATED	coord: 121..323
None	No IPR available	PANTHER	PTHR45968	OSJNBA0019K04.7 PROTEIN	coord: 121..323
None	No IPR available	PANTHER	PTHR45968	OSJNBA0019K04.7 PROTEIN	coord: 1..78
None	No IPR available	PANTHER	PTHR45968	OSJNBA0019K04.7 PROTEIN	coord: 79..119
None	No IPR available	PANTHER	PTHR45968:SF23	(R)-MANDELONITRILE LYASE 4-RELATED	coord: 1..78
None	No IPR available	PANTHER	PTHR45968:SF23	(R)-MANDELONITRILE LYASE 4-RELATED	coord: 79..119
None	No IPR available	SUPERFAMILY	54373	FAD-linked reductases, C-terminal domain	coord: 173..273
IPR000172	Glucose-methanol-choline oxidoreductase, N-terminal	PFAM	PF00732	GMC_oxred_N	coord: 79..118 e-value: 2.1E-4 score: 20.8
IPR007867	Glucose-methanol-choline oxidoreductase, C-terminal	PFAM	PF05199	GMC_oxred_C	coord: 176..317 e-value: 3.0E-28 score: 99.1

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi11G007430.1	Lsi11G007430.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0050660	flavin adenine dinucleotide binding
molecular_function	GO:0016614	oxidoreductase activity, acting on CH-OH group of donors