Lsi11G006580 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi11G006580
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionGlucose-6-phosphate 1-dehydrogenase
Locationchr11: 6903401 .. 6905830 (+)
RNA-Seq ExpressionLsi11G006580
SyntenyLsi11G006580
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGTCGTACATTCATAAACAGCGTACAGGTACACATTTCTATAAAGCAAATTCTAGTCCTTTCACACTCAATACTCACTAAATAAATTAAAAAATATTACAGGTCATTTTGTCAGAAGAACTTGGAGTTCAAACAAAAAGGTTACCTGGCAGTTCACTGGTTTTACTTTTACCGTTGTCTTGAATTATTGTTACAGGATCATCTACAAGTATTCAGTTCCTTCCATGCAGATTTTTGGACGGTGGTGGGATCATTGGAGACATAGTACACAGCCATATACTACAAACAATCGCATTACTTGCCATGGAACCACCCATTAGTCTTGATGGTGAAGCCATTCGAAATGAGAAGGTAAATATAGAATTCTCAAGAAAGTACTCTTGCATGGTATAATCACAGTAATGTAAATGAATAGATTCTTTCCAGGTTAAGCTGCTGAGATCCATTCGCCCATTGGAACCCAGTGATGTTGTTCTCGGACAGTACAAATCTAGTACTAATGACAAAGTTGACCTTTCTCTCGACAATCTGACCCCGACATATTTCGCGGGTGCTTTGTACATTGACAATGCACGTTGGGATGGCGTCCCTTTTTTGATCAAAAGTGGCTTGGGACTAATCAAACACTGGTAAATTTTTCTTTGGTAAGGCTATTGCATGTCAGAAAGATGTAGGACTGGGATACACTTAGTTGCCATCCCAAGAACATATGACAATGTAATGGATGATTTTTTCCTCTCTTTTTTGTTATTACACATGCATCCATGGGAACCTATTGATAAGAGGGGAATGAGAAATAGAAGAAAAGAAGTACCATTTATGAGAGAAATTAAGAACCAAAAAGTTGAAAGGAAGGAAAACTTGAATAGTTGAAATATCAACCTCAGTTTCAAATACTAAGGGCATATGAATGTTAATAGCTTAAGCAAACTATTATGTGAAATCTTATTTTTATCCGAGCTGGTTCTGAACACGAGTTAAAAATTCCAGAAAATGATGATTTAAAATCTCTGATATGGTCATAATTTTAATTCTTCTTCTTATTCTCTGCTTAACTTGCATTCTGATTTGAGATTTGTTTATTTTTACAAGTCAACTGTTAGAATTGGTCCACCACTACCAACTTTGAAATATATAGTCCTTGATGGAGAATTGCTCTGAAATGCAGTGTAGAGATCCGTATACAATTTCGCCAGGTTCCGGGGAACATTTATCGTGAGCACATTGGGTATAATAATGAATCTGCAACTAACGAGATTATTTTGCGCGATGCACCTGAAGAGGCCATTCTTGTCCGAGTTAATAACAAGGTTCCTGGATTGGGATTGCGATTGGACTCTCCAGAGCTCAATTTGCTTTACAAAGACAAGTGCGTCCGTGCTTTGAATTTGAAATATTGTCCTTCGTGATTTCTAGTTGCAGCTTGGTTTTGATGTAGACATTGTTTTATTCTCTTTTATCCATTTTTGTTTTTCTCCTTTGTAGGTACAACGTGGACGTGCCTGATTCATATGAACATCTTCTCCTTGATGTCATTGATGGAGATAGCCATCTGTTCATGAGAAGTGATGAGCTCGCAATTGCGTGGAACATCCTAACTCCGATCCTGAACGAAATCAAGAAGAATAACATAAAGCCAGAACTCTACGAGTTTGGTGGCAGAGGTCCTATCGGAGCTTATTATCTTTGGGCAAAACACGGAGTTCGATGGGTCGAGGAGTAAGCAACAGGACATTTCATTCTGTGGCAAGCTCTCTCACAAGAAATAGGCACACACAAGATTTGCCAAATGGTTGAATCATTACATTCATCATCATCCAGTTAGTTCAATCAATTGAAGGAAGCAGTCTTGGACATAAAATTTGAACCTAGTAGAATTAAAACCACATGATGATGCAACTCCTGGTTTTGATTCCTTGTGATGTTCATAGGAGTAATCTGTCATTTGAAAGAATATGAGGATTGTACTGGACCATACCATATAAACAGAGTCATTGCACAAAAGTTCATTCTGTAAGAGAAGAAACTGTATCCATCTGTATATTTTTCTAATTTTCAATGCAGAATGATTTTAGCCCAAGTTGATGTCAAATTGTAGCAGAAATGAAGAATATGAAGAGCATAATATGGACCCAGGTAATAGATTCATTGAACTTTTTCCTTTTAGTTTGGTTTTATCCAATCCTTTTTAATTATTTATGTTTTATTAGTGGCAGCCATACAGAGAGTGTAGGATCCACAAAGACTTGGAGATATTCCAGTCAACTAATAAAAACAGGCCGCCATGAAATTGGTGGAAATTAAGGGAAAAAGAATGCCCATGATCCAGACACTCATGATGTCTCATTGTTCATTTTGGGGTCGGATAAGGATATATATATATATATTAAATCTTGTTTTAATCCTTGATTTTTTATTTTTTATTTT

mRNA sequence

ATGGAGTCGTACATTCATAAACAGCGTACAGGATCATCTACAAGTATTCAGTTCCTTCCATGCAGATTTTTGGACGGTGGTGGGATCATTGGAGACATAGTACACAGCCATATACTACAAACAATCGCATTACTTGCCATGGAACCACCCATTAGTCTTGATGGTGAAGCCATTCGAAATGAGAAGGTTAAGCTGCTGAGATCCATTCGCCCATTGGAACCCAGTGATGTTGTTCTCGGACAGTACAAATCTAGTACTAATGACAAAGTTGACCTTTCTCTCGACAATCTGACCCCGACATATTTCGCGGGTGCTTTGTACATTGACAATGCACGTTGGGATGGCGTCCCTTTTTTGATCAAAAGTGGCTTGGGACTAATCAAACACTGTGTAGAGATCCGTATACAATTTCGCCAGGTTCCGGGGAACATTTATCGTGAGCACATTGGGTATAATAATGAATCTGCAACTAACGAGATTATTTTGCGCGATGCACCTGAAGAGGCCATTCTTGTCCGAGTTAATAACAAGGTTCCTGGATTGGGATTGCGATTGGACTCTCCAGAGCTCAATTTGCTTTACAAAGACAAGTACAACGTGGACGTGCCTGATTCATATGAACATCTTCTCCTTGATGTCATTGATGGAGATAGCCATCTGTTCATGAGAAGTGATGAGCTCGCAATTGCGTGGAACATCCTAACTCCGATCCTGAACGAAATCAAGAAGAATAACATAAAGCCAGAACTCTACGAGTTTGGTGGCAGAGGTCCTATCGGAGCTTATTATCTTTGGGCAAAACACGGAGTTCGATGGGTCGAGGAGTAAGCAACAGGACATTTCATTCTGTGGCAAGCTCTCTCACAAGAAATAGGCACACACAAGATTTGCCAAATGGTTGAATCATTACATTCATCATCATCCAGTTAGTTCAATCAATTGAAGGAAGCAGTCTTGGACATAAAATTTGAACCTAGTAGAATTAAAACCACATGATGATGCAACTCCTGGTTTTGATTCCTTGTGATGTTCATAGGAGTAATCTGTCATTTGAAAGAATATGAGGATTGTACTGGACCATACCATATAAACAGAGTCATTGCACAAAAGTTCATTCTGTAAGAGAAGAAACTGTATCCATCTGTATATTTTTCTAATTTTCAATGCAGAATGATTTTAGCCCAAGTTGATGTCAAATTGTAGCAGAAATGAAGAATATGAAGAGCATAATATGGACCCAGTGGCAGCCATACAGAGAGTGTAGGATCCACAAAGACTTGGAGATATTCCAGTCAACTAATAAAAACAGGCCGCCATGAAATTGGTGGAAATTAAGGGAAAAAGAATGCCCATGATCCAGACACTCATGATGTCTCATTGTTCATTTTGGGGTCGGATAAGGATATATATATATATATTAAATCTTGTTTTAATCCTTGATTTTTTATTTTTTATTTT

Coding sequence (CDS)

ATGGAGTCGTACATTCATAAACAGCGTACAGGATCATCTACAAGTATTCAGTTCCTTCCATGCAGATTTTTGGACGGTGGTGGGATCATTGGAGACATAGTACACAGCCATATACTACAAACAATCGCATTACTTGCCATGGAACCACCCATTAGTCTTGATGGTGAAGCCATTCGAAATGAGAAGGTTAAGCTGCTGAGATCCATTCGCCCATTGGAACCCAGTGATGTTGTTCTCGGACAGTACAAATCTAGTACTAATGACAAAGTTGACCTTTCTCTCGACAATCTGACCCCGACATATTTCGCGGGTGCTTTGTACATTGACAATGCACGTTGGGATGGCGTCCCTTTTTTGATCAAAAGTGGCTTGGGACTAATCAAACACTGTGTAGAGATCCGTATACAATTTCGCCAGGTTCCGGGGAACATTTATCGTGAGCACATTGGGTATAATAATGAATCTGCAACTAACGAGATTATTTTGCGCGATGCACCTGAAGAGGCCATTCTTGTCCGAGTTAATAACAAGGTTCCTGGATTGGGATTGCGATTGGACTCTCCAGAGCTCAATTTGCTTTACAAAGACAAGTACAACGTGGACGTGCCTGATTCATATGAACATCTTCTCCTTGATGTCATTGATGGAGATAGCCATCTGTTCATGAGAAGTGATGAGCTCGCAATTGCGTGGAACATCCTAACTCCGATCCTGAACGAAATCAAGAAGAATAACATAAAGCCAGAACTCTACGAGTTTGGTGGCAGAGGTCCTATCGGAGCTTATTATCTTTGGGCAAAACACGGAGTTCGATGGGTCGAGGAGTAA

Protein sequence

MESYIHKQRTGSSTSIQFLPCRFLDGGGIIGDIVHSHILQTIALLAMEPPISLDGEAIRNEKVKLLRSIRPLEPSDVVLGQYKSSTNDKVDLSLDNLTPTYFAGALYIDNARWDGVPFLIKSGLGLIKHCVEIRIQFRQVPGNIYREHIGYNNESATNEIILRDAPEEAILVRVNNKVPGLGLRLDSPELNLLYKDKYNVDVPDSYEHLLLDVIDGDSHLFMRSDELAIAWNILTPILNEIKKNNIKPELYEFGGRGPIGAYYLWAKHGVRWVEE
Homology
BLAST of Lsi11G006580 vs. ExPASy Swiss-Prot
Match: Q93ZW0 (Inactive glucose-6-phosphate 1-dehydrogenase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=G6PD4 PE=1 SV=1)

HSP 1 Score: 399.1 bits (1024), Expect = 4.2e-110
Identity = 185/254 (72.83%), Postives = 218/254 (85.83%), Query Frame = 0

Query: 22  RFLDGGGIIGDIVHSHILQTIALLAMEPPISLDGEAIRNEKVKLLRSIRPLEPSDVVLGQ 81
           +F DG GII DIVHSHILQTIALLAMEPPISLDGE IRNEKVK+LRSIR ++P DV+LGQ
Sbjct: 372 KFSDGYGIIRDIVHSHILQTIALLAMEPPISLDGEDIRNEKVKVLRSIRKIDPRDVILGQ 431

Query: 82  YKSSTNDKVDLSLDNLTPTYFAGALYIDNARWDGVPFLIKSGLGLIKHCVEIRIQFRQVP 141
           YKSS+ DK  + L+ + PTY A ALYIDNARWDGVPFL++ G GLIKH VEI +QFR VP
Sbjct: 432 YKSSSRDKNGVILNGVDPTYCAAALYIDNARWDGVPFLVRVGTGLIKHRVEIHVQFRHVP 491

Query: 142 GNIYREHIGYNNESATNEIILRDAPEEAILVRVNNKVPGLGLRLDSPELNLLYKDKYNVD 201
           GN+YRE+IG N +  TNE+ILRD P+EAILV++NNKVPGLGL+LD+ ELNLLYKD+Y  +
Sbjct: 492 GNLYRENIGINIDLGTNELILRDEPDEAILVKINNKVPGLGLQLDASELNLLYKDRYKTE 551

Query: 202 VPDSYEHLLLDVIDGDSHLFMRSDELAIAWNILTPILNEIKKNNIKPELYEFGGRGPIGA 261
           VPDSYEHL+ DVIDGD+HLFMRSDE+A AWNIL+P+L EI K++  PELYEFGGRGP+ A
Sbjct: 552 VPDSYEHLIHDVIDGDNHLFMRSDEVAAAWNILSPVLEEIDKHHTAPELYEFGGRGPVAA 611

Query: 262 YYLWAKHGVRWVEE 276
           YYLWAKHGV W ++
Sbjct: 612 YYLWAKHGVPWADD 625

BLAST of Lsi11G006580 vs. ExPASy Swiss-Prot
Match: Q43839 (Glucose-6-phosphate 1-dehydrogenase, chloroplastic OS=Solanum tuberosum OX=4113 PE=1 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 4.4e-83
Identity = 146/259 (56.37%), Postives = 191/259 (73.75%), Query Frame = 0

Query: 23  FLDGGGIIGDIVHSHILQTIALLAMEPPISLDGEAIRNEKVKLLRSIRPLEPSDVVLGQY 82
           + D  GII DI+ +H+LQ +AL AME P+SLD E IRNEKVK+LRS+RPL+  DVVLGQY
Sbjct: 312 YFDHYGIIRDIMQNHLLQILALFAMETPVSLDAEDIRNEKVKVLRSMRPLQLEDVVLGQY 371

Query: 83  KSSTN---------DKVDLSLDNLTPTYFAGALYIDNARWDGVPFLIKSGLGLIKHCVEI 142
           K  +N         D   +   ++TPT+ A AL+IDNARWDGVPFL+K+G  L     EI
Sbjct: 372 KGHSNGAKSYPAYTDDPTVPNGSITPTFSAAALFIDNARWDGVPFLMKAGKALHTKRAEI 431

Query: 143 RIQFRQVPGNIYREHIGYNNESATNEIILRDAPEEAILVRVNNKVPGLGLRLDSPELNLL 202
           R+QFR VPGN+Y+ + G + + ATNE++LR  P+EAI +++NNKVPGLG+RLD  +LNLL
Sbjct: 432 RVQFRHVPGNLYKRNFGTDMDKATNELVLRLQPDEAIYLKINNKVPGLGMRLDRSDLNLL 491

Query: 203 YKDKYNVDVPDSYEHLLLDVIDGDSHLFMRSDELAIAWNILTPILNEIKKNNIKPELYEF 262
           YK KY  ++PD+YE LLLD I+G+  LF+RSDEL  AW + TP+L E+++  I PELY +
Sbjct: 492 YKAKYRGEIPDAYERLLLDAIEGERRLFIRSDELDAAWALFTPLLKELEEKKIAPELYPY 551

Query: 263 GGRGPIGAYYLWAKHGVRW 273
           G RGP+GA+YL AKH VRW
Sbjct: 552 GSRGPVGAHYLAAKHNVRW 570

BLAST of Lsi11G006580 vs. ExPASy Swiss-Prot
Match: Q43793 (Glucose-6-phosphate 1-dehydrogenase, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 301.6 bits (771), Expect = 9.1e-81
Identity = 141/259 (54.44%), Postives = 188/259 (72.59%), Query Frame = 0

Query: 23  FLDGGGIIGDIVHSHILQTIALLAMEPPISLDGEAIRNEKVKLLRSIRPLEPSDVVLGQY 82
           + D  GII DI+ +H+LQ +AL AME P+SLD E IRNEKVK+LRS+RPL+  DV++GQY
Sbjct: 331 YFDHYGIIRDIMQNHLLQILALFAMETPVSLDAEDIRNEKVKVLRSMRPLQLDDVIIGQY 390

Query: 83  KSST---------NDKVDLSLDNLTPTYFAGALYIDNARWDGVPFLIKSGLGLIKHCVEI 142
           K  T          D   +  D+LTPT+ A AL+IDNARWDGVPFL+K+G  L     EI
Sbjct: 391 KCHTKGDVTYPGYTDDKTVPKDSLTPTFAAAALFIDNARWDGVPFLMKAGKALHTRSAEI 450

Query: 143 RIQFRQVPGNIYREHIGYNNESATNEIILRDAPEEAILVRVNNKVPGLGLRLDSPELNLL 202
           R+QFR VPGN+Y ++ G + + ATNE+++R  P EAI +++NNKVPGLG+RLD   LNLL
Sbjct: 451 RVQFRHVPGNLYNKNFGSDLDQATNELVIRVQPNEAIYLKINNKVPGLGMRLDRSNLNLL 510

Query: 203 YKDKYNVDVPDSYEHLLLDVIDGDSHLFMRSDELAIAWNILTPILNEIKKNNIKPELYEF 262
           Y  +Y+ ++PD+YE LLLD I+G+  LF+RSDEL  AW++ TP+L E++   I PE Y +
Sbjct: 511 YSARYSKEIPDAYERLLLDAIEGERRLFIRSDELDAAWSLFTPVLKELEDKKIVPEYYPY 570

Query: 263 GGRGPIGAYYLWAKHGVRW 273
           G RGPIGA+YL A++ VRW
Sbjct: 571 GSRGPIGAHYLAARYKVRW 589

BLAST of Lsi11G006580 vs. ExPASy Swiss-Prot
Match: Q9FY99 (Glucose-6-phosphate 1-dehydrogenase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=G6PD2 PE=1 SV=2)

HSP 1 Score: 300.4 bits (768), Expect = 2.0e-80
Identity = 141/259 (54.44%), Postives = 187/259 (72.20%), Query Frame = 0

Query: 23  FLDGGGIIGDIVHSHILQTIALLAMEPPISLDGEAIRNEKVKLLRSIRPLEPSDVVLGQY 82
           + D  GII DI+ +H+LQ +AL AME P+SLD E IRNEKVK+LRS+RP+   DVV+GQY
Sbjct: 331 YFDNYGIIRDIMQNHLLQILALFAMETPVSLDAEDIRNEKVKVLRSMRPIRVEDVVIGQY 390

Query: 83  KSST---------NDKVDLSLDNLTPTYFAGALYIDNARWDGVPFLIKSGLGLIKHCVEI 142
           KS T          D   +   +LTPT+ A AL+IDNARWDGVPFL+K+G  L     EI
Sbjct: 391 KSHTKGGVTYPAYTDDKTVPKGSLTPTFAAAALFIDNARWDGVPFLMKAGKALHTRSAEI 450

Query: 143 RIQFRQVPGNIYREHIGYNNESATNEIILRDAPEEAILVRVNNKVPGLGLRLDSPELNLL 202
           R+QFR VPGN+Y  + G + + ATNE+++R  P+EAI +++NNKVPGLG+RLD   LNLL
Sbjct: 451 RVQFRHVPGNLYNRNTGSDLDQATNELVIRVQPDEAIYLKINNKVPGLGMRLDRSNLNLL 510

Query: 203 YKDKYNVDVPDSYEHLLLDVIDGDSHLFMRSDELAIAWNILTPILNEIKKNNIKPELYEF 262
           Y  +Y+ ++PD+YE LLLD I+G+  LF+RSDEL  AW++ TP+L EI++    PE Y +
Sbjct: 511 YSARYSKEIPDAYERLLLDAIEGERRLFIRSDELDAAWSLFTPLLKEIEEKKRIPEYYPY 570

Query: 263 GGRGPIGAYYLWAKHGVRW 273
           G RGP+GA+YL AKH V+W
Sbjct: 571 GSRGPVGAHYLAAKHKVQW 589

BLAST of Lsi11G006580 vs. ExPASy Swiss-Prot
Match: Q8L743 (Glucose-6-phosphate 1-dehydrogenase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=G6PD3 PE=1 SV=2)

HSP 1 Score: 300.1 bits (767), Expect = 2.6e-80
Identity = 140/259 (54.05%), Postives = 186/259 (71.81%), Query Frame = 0

Query: 23  FLDGGGIIGDIVHSHILQTIALLAMEPPISLDGEAIRNEKVKLLRSIRPLEPSDVVLGQY 82
           + D  GII DI+ +H+LQ +AL AME P+SLD E IRNEKVK+LRS+RP++  DVV+GQY
Sbjct: 334 YFDNYGIIRDIMQNHLLQILALFAMETPVSLDAEDIRNEKVKVLRSMRPIKLEDVVIGQY 393

Query: 83  KS---------STNDKVDLSLDNLTPTYFAGALYIDNARWDGVPFLIKSGLGLIKHCVEI 142
           KS         S  D   +   +LTPT+ A AL+IDNARWDGVPFL+K+G  L     EI
Sbjct: 394 KSHSIGGVTYPSYTDDKTVPKGSLTPTFAAAALFIDNARWDGVPFLMKAGKALNTRSAEI 453

Query: 143 RIQFRQVPGNIYREHIGYNNESATNEIILRDAPEEAILVRVNNKVPGLGLRLDSPELNLL 202
           R+QFR VPGN+Y  + G + +  TNE+++R  P+EAI +++NNKVPGLG+RLD   LNLL
Sbjct: 454 RVQFRHVPGNLYNRNSGTDRDQTTNELVIRVQPDEAIYLKINNKVPGLGMRLDQSNLNLL 513

Query: 203 YKDKYNVDVPDSYEHLLLDVIDGDSHLFMRSDELAIAWNILTPILNEIKKNNIKPELYEF 262
           Y  +Y+ ++PD+YE LLLD I+G+  LF+RSDEL  AW + TP+L EI++    PE Y +
Sbjct: 514 YSARYSKEIPDAYERLLLDAIEGERRLFIRSDELDAAWALFTPLLKEIEEKKTTPEFYPY 573

Query: 263 GGRGPIGAYYLWAKHGVRW 273
           G RGP+GA+YL AKH V+W
Sbjct: 574 GSRGPVGAHYLAAKHKVQW 592

BLAST of Lsi11G006580 vs. ExPASy TrEMBL
Match: A0A0A0LH10 (Glucose-6-phosphate 1-dehydrogenase OS=Cucumis sativus OX=3659 GN=Csa_2G034760 PE=3 SV=1)

HSP 1 Score: 520.8 bits (1340), Expect = 3.5e-144
Identity = 251/254 (98.82%), Postives = 252/254 (99.21%), Query Frame = 0

Query: 22  RFLDGGGIIGDIVHSHILQTIALLAMEPPISLDGEAIRNEKVKLLRSIRPLEPSDVVLGQ 81
           RFLDGGGIIGDIVHSHILQTIALLAMEPPISLDGEAIRNEKVKLLRSIRPLEPSDVVLGQ
Sbjct: 385 RFLDGGGIIGDIVHSHILQTIALLAMEPPISLDGEAIRNEKVKLLRSIRPLEPSDVVLGQ 444

Query: 82  YKSSTNDKVDLSLDNLTPTYFAGALYIDNARWDGVPFLIKSGLGLIKHCVEIRIQFRQVP 141
           YKSSTNDKVDLSLDNLTPTYFAGALYIDNARWDGVPFLIKSGLGLIKHCVEIRIQFRQVP
Sbjct: 445 YKSSTNDKVDLSLDNLTPTYFAGALYIDNARWDGVPFLIKSGLGLIKHCVEIRIQFRQVP 504

Query: 142 GNIYREHIGYNNESATNEIILRDAPEEAILVRVNNKVPGLGLRLDSPELNLLYKDKYNVD 201
           GNIYREH GYNNESATNEIILRD PEEAILVRVNNK+PGLGLRLDSPELNLLYKDKYNVD
Sbjct: 505 GNIYREHFGYNNESATNEIILRDLPEEAILVRVNNKIPGLGLRLDSPELNLLYKDKYNVD 564

Query: 202 VPDSYEHLLLDVIDGDSHLFMRSDELAIAWNILTPILNEIKKNNIKPELYEFGGRGPIGA 261
           VPDSYEHLLLDVIDGDSHLFMRSDELAIAWNILTPILNEIKKNNIKPELYEFGGRGPIGA
Sbjct: 565 VPDSYEHLLLDVIDGDSHLFMRSDELAIAWNILTPILNEIKKNNIKPELYEFGGRGPIGA 624

Query: 262 YYLWAKHGVRWVEE 276
           YYLWAKHGVRWVEE
Sbjct: 625 YYLWAKHGVRWVEE 638

BLAST of Lsi11G006580 vs. ExPASy TrEMBL
Match: A0A5A7U1K2 (Glucose-6-phosphate 1-dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold385G001950 PE=3 SV=1)

HSP 1 Score: 519.6 bits (1337), Expect = 7.8e-144
Identity = 252/262 (96.18%), Postives = 256/262 (97.71%), Query Frame = 0

Query: 15  SIQFLPC-RFLDGGGIIGDIVHSHILQTIALLAMEPPISLDGEAIRNEKVKLLRSIRPLE 74
           S+Q   C +FLDGGGIIGDIVHSHILQTIALLAMEPPISLDGEAIRNEKVKLLRSIRPLE
Sbjct: 370 SVQVHICIKFLDGGGIIGDIVHSHILQTIALLAMEPPISLDGEAIRNEKVKLLRSIRPLE 429

Query: 75  PSDVVLGQYKSSTNDKVDLSLDNLTPTYFAGALYIDNARWDGVPFLIKSGLGLIKHCVEI 134
           PSDVVLGQYKSST+DKVDLSLDNLTPTYFAGALYIDNARWDGVPFLIKSGLGLIKHCVEI
Sbjct: 430 PSDVVLGQYKSSTDDKVDLSLDNLTPTYFAGALYIDNARWDGVPFLIKSGLGLIKHCVEI 489

Query: 135 RIQFRQVPGNIYREHIGYNNESATNEIILRDAPEEAILVRVNNKVPGLGLRLDSPELNLL 194
           RIQFRQVPGNIYREH GYNNESATNEIILRD PEEAILVRVNNK+PGLGLRLDSPELNLL
Sbjct: 490 RIQFRQVPGNIYREHFGYNNESATNEIILRDVPEEAILVRVNNKIPGLGLRLDSPELNLL 549

Query: 195 YKDKYNVDVPDSYEHLLLDVIDGDSHLFMRSDELAIAWNILTPILNEIKKNNIKPELYEF 254
           YKDKYNVDVPDSYEHLLLDVIDGDSHLFMRSDELAIAWNILTPILNEIKKNNIKPELYEF
Sbjct: 550 YKDKYNVDVPDSYEHLLLDVIDGDSHLFMRSDELAIAWNILTPILNEIKKNNIKPELYEF 609

Query: 255 GGRGPIGAYYLWAKHGVRWVEE 276
           GGRGPIGAYYLWAKHGVRWVEE
Sbjct: 610 GGRGPIGAYYLWAKHGVRWVEE 631

BLAST of Lsi11G006580 vs. ExPASy TrEMBL
Match: A0A5D3BC05 (Glucose-6-phosphate 1-dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G00700 PE=3 SV=1)

HSP 1 Score: 519.2 bits (1336), Expect = 1.0e-143
Identity = 250/254 (98.43%), Postives = 252/254 (99.21%), Query Frame = 0

Query: 22  RFLDGGGIIGDIVHSHILQTIALLAMEPPISLDGEAIRNEKVKLLRSIRPLEPSDVVLGQ 81
           RFLDGGGIIGDIVHSHILQTIALLAMEPPISLDGEAIRNEKVKLLRSIRPLEPSDVVLGQ
Sbjct: 385 RFLDGGGIIGDIVHSHILQTIALLAMEPPISLDGEAIRNEKVKLLRSIRPLEPSDVVLGQ 444

Query: 82  YKSSTNDKVDLSLDNLTPTYFAGALYIDNARWDGVPFLIKSGLGLIKHCVEIRIQFRQVP 141
           YKSST+DKVDLSLDNLTPTYFAGALYIDNARWDGVPFLIKSGLGLIKHCVEIRIQFRQVP
Sbjct: 445 YKSSTDDKVDLSLDNLTPTYFAGALYIDNARWDGVPFLIKSGLGLIKHCVEIRIQFRQVP 504

Query: 142 GNIYREHIGYNNESATNEIILRDAPEEAILVRVNNKVPGLGLRLDSPELNLLYKDKYNVD 201
           GNIYREH GYNNESATNEIILRD PEEAILVRVNNK+PGLGLRLDSPELNLLYKDKYNVD
Sbjct: 505 GNIYREHFGYNNESATNEIILRDVPEEAILVRVNNKIPGLGLRLDSPELNLLYKDKYNVD 564

Query: 202 VPDSYEHLLLDVIDGDSHLFMRSDELAIAWNILTPILNEIKKNNIKPELYEFGGRGPIGA 261
           VPDSYEHLLLDVIDGDSHLFMRSDELAIAWNILTPILNEIKKNNIKPELYEFGGRGPIGA
Sbjct: 565 VPDSYEHLLLDVIDGDSHLFMRSDELAIAWNILTPILNEIKKNNIKPELYEFGGRGPIGA 624

Query: 262 YYLWAKHGVRWVEE 276
           YYLWAKHGVRWVEE
Sbjct: 625 YYLWAKHGVRWVEE 638

BLAST of Lsi11G006580 vs. ExPASy TrEMBL
Match: A0A1S3BVM7 (Glucose-6-phosphate 1-dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103493988 PE=3 SV=1)

HSP 1 Score: 519.2 bits (1336), Expect = 1.0e-143
Identity = 250/254 (98.43%), Postives = 252/254 (99.21%), Query Frame = 0

Query: 22  RFLDGGGIIGDIVHSHILQTIALLAMEPPISLDGEAIRNEKVKLLRSIRPLEPSDVVLGQ 81
           RFLDGGGIIGDIVHSHILQTIALLAMEPPISLDGEAIRNEKVKLLRSIRPLEPSDVVLGQ
Sbjct: 385 RFLDGGGIIGDIVHSHILQTIALLAMEPPISLDGEAIRNEKVKLLRSIRPLEPSDVVLGQ 444

Query: 82  YKSSTNDKVDLSLDNLTPTYFAGALYIDNARWDGVPFLIKSGLGLIKHCVEIRIQFRQVP 141
           YKSST+DKVDLSLDNLTPTYFAGALYIDNARWDGVPFLIKSGLGLIKHCVEIRIQFRQVP
Sbjct: 445 YKSSTDDKVDLSLDNLTPTYFAGALYIDNARWDGVPFLIKSGLGLIKHCVEIRIQFRQVP 504

Query: 142 GNIYREHIGYNNESATNEIILRDAPEEAILVRVNNKVPGLGLRLDSPELNLLYKDKYNVD 201
           GNIYREH GYNNESATNEIILRD PEEAILVRVNNK+PGLGLRLDSPELNLLYKDKYNVD
Sbjct: 505 GNIYREHFGYNNESATNEIILRDVPEEAILVRVNNKIPGLGLRLDSPELNLLYKDKYNVD 564

Query: 202 VPDSYEHLLLDVIDGDSHLFMRSDELAIAWNILTPILNEIKKNNIKPELYEFGGRGPIGA 261
           VPDSYEHLLLDVIDGDSHLFMRSDELAIAWNILTPILNEIKKNNIKPELYEFGGRGPIGA
Sbjct: 565 VPDSYEHLLLDVIDGDSHLFMRSDELAIAWNILTPILNEIKKNNIKPELYEFGGRGPIGA 624

Query: 262 YYLWAKHGVRWVEE 276
           YYLWAKHGVRWVEE
Sbjct: 625 YYLWAKHGVRWVEE 638

BLAST of Lsi11G006580 vs. ExPASy TrEMBL
Match: A0A6J1KGT2 (Glucose-6-phosphate 1-dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111493753 PE=3 SV=1)

HSP 1 Score: 506.9 bits (1304), Expect = 5.3e-140
Identity = 244/254 (96.06%), Postives = 248/254 (97.64%), Query Frame = 0

Query: 22  RFLDGGGIIGDIVHSHILQTIALLAMEPPISLDGEAIRNEKVKLLRSIRPLEPSDVVLGQ 81
           RFLDGGGIIGDIVHSHILQTIALLAMEPPISLDGEAIRNEKVKLLRSIRPLEPSDVVLGQ
Sbjct: 380 RFLDGGGIIGDIVHSHILQTIALLAMEPPISLDGEAIRNEKVKLLRSIRPLEPSDVVLGQ 439

Query: 82  YKSSTNDKVDLSLDNLTPTYFAGALYIDNARWDGVPFLIKSGLGLIKHCVEIRIQFRQVP 141
           YKSS  DKVDL LD+LTPTYFAGALYIDNARWDGVPFLIKSGLGLIKHCVEIRIQFRQVP
Sbjct: 440 YKSSAKDKVDLFLDDLTPTYFAGALYIDNARWDGVPFLIKSGLGLIKHCVEIRIQFRQVP 499

Query: 142 GNIYREHIGYNNESATNEIILRDAPEEAILVRVNNKVPGLGLRLDSPELNLLYKDKYNVD 201
           GNIYREHIGYN+ S TNEIILRD PEEAILVRVNNKVPGLGLRLDSPELNLLYKDKYNVD
Sbjct: 500 GNIYREHIGYNSASTTNEIILRDVPEEAILVRVNNKVPGLGLRLDSPELNLLYKDKYNVD 559

Query: 202 VPDSYEHLLLDVIDGDSHLFMRSDELAIAWNILTPILNEIKKNNIKPELYEFGGRGPIGA 261
           VPDSYEHLLLDVIDGDSHLFMRSDELA+AWNILTPILNEIK+NNIKPELYEFGGRGPIGA
Sbjct: 560 VPDSYEHLLLDVIDGDSHLFMRSDELAVAWNILTPILNEIKENNIKPELYEFGGRGPIGA 619

Query: 262 YYLWAKHGVRWVEE 276
           YYLWAKHGVRWVEE
Sbjct: 620 YYLWAKHGVRWVEE 633

BLAST of Lsi11G006580 vs. NCBI nr
Match: KAE8651631.1 (hypothetical protein Csa_021325 [Cucumis sativus])

HSP 1 Score: 520.8 bits (1340), Expect = 7.3e-144
Identity = 251/254 (98.82%), Postives = 252/254 (99.21%), Query Frame = 0

Query: 22  RFLDGGGIIGDIVHSHILQTIALLAMEPPISLDGEAIRNEKVKLLRSIRPLEPSDVVLGQ 81
           RFLDGGGIIGDIVHSHILQTIALLAMEPPISLDGEAIRNEKVKLLRSIRPLEPSDVVLGQ
Sbjct: 363 RFLDGGGIIGDIVHSHILQTIALLAMEPPISLDGEAIRNEKVKLLRSIRPLEPSDVVLGQ 422

Query: 82  YKSSTNDKVDLSLDNLTPTYFAGALYIDNARWDGVPFLIKSGLGLIKHCVEIRIQFRQVP 141
           YKSSTNDKVDLSLDNLTPTYFAGALYIDNARWDGVPFLIKSGLGLIKHCVEIRIQFRQVP
Sbjct: 423 YKSSTNDKVDLSLDNLTPTYFAGALYIDNARWDGVPFLIKSGLGLIKHCVEIRIQFRQVP 482

Query: 142 GNIYREHIGYNNESATNEIILRDAPEEAILVRVNNKVPGLGLRLDSPELNLLYKDKYNVD 201
           GNIYREH GYNNESATNEIILRD PEEAILVRVNNK+PGLGLRLDSPELNLLYKDKYNVD
Sbjct: 483 GNIYREHFGYNNESATNEIILRDLPEEAILVRVNNKIPGLGLRLDSPELNLLYKDKYNVD 542

Query: 202 VPDSYEHLLLDVIDGDSHLFMRSDELAIAWNILTPILNEIKKNNIKPELYEFGGRGPIGA 261
           VPDSYEHLLLDVIDGDSHLFMRSDELAIAWNILTPILNEIKKNNIKPELYEFGGRGPIGA
Sbjct: 543 VPDSYEHLLLDVIDGDSHLFMRSDELAIAWNILTPILNEIKKNNIKPELYEFGGRGPIGA 602

Query: 262 YYLWAKHGVRWVEE 276
           YYLWAKHGVRWVEE
Sbjct: 603 YYLWAKHGVRWVEE 616

BLAST of Lsi11G006580 vs. NCBI nr
Match: XP_004144740.1 (inactive glucose-6-phosphate 1-dehydrogenase 4, chloroplastic [Cucumis sativus])

HSP 1 Score: 520.8 bits (1340), Expect = 7.3e-144
Identity = 251/254 (98.82%), Postives = 252/254 (99.21%), Query Frame = 0

Query: 22  RFLDGGGIIGDIVHSHILQTIALLAMEPPISLDGEAIRNEKVKLLRSIRPLEPSDVVLGQ 81
           RFLDGGGIIGDIVHSHILQTIALLAMEPPISLDGEAIRNEKVKLLRSIRPLEPSDVVLGQ
Sbjct: 385 RFLDGGGIIGDIVHSHILQTIALLAMEPPISLDGEAIRNEKVKLLRSIRPLEPSDVVLGQ 444

Query: 82  YKSSTNDKVDLSLDNLTPTYFAGALYIDNARWDGVPFLIKSGLGLIKHCVEIRIQFRQVP 141
           YKSSTNDKVDLSLDNLTPTYFAGALYIDNARWDGVPFLIKSGLGLIKHCVEIRIQFRQVP
Sbjct: 445 YKSSTNDKVDLSLDNLTPTYFAGALYIDNARWDGVPFLIKSGLGLIKHCVEIRIQFRQVP 504

Query: 142 GNIYREHIGYNNESATNEIILRDAPEEAILVRVNNKVPGLGLRLDSPELNLLYKDKYNVD 201
           GNIYREH GYNNESATNEIILRD PEEAILVRVNNK+PGLGLRLDSPELNLLYKDKYNVD
Sbjct: 505 GNIYREHFGYNNESATNEIILRDLPEEAILVRVNNKIPGLGLRLDSPELNLLYKDKYNVD 564

Query: 202 VPDSYEHLLLDVIDGDSHLFMRSDELAIAWNILTPILNEIKKNNIKPELYEFGGRGPIGA 261
           VPDSYEHLLLDVIDGDSHLFMRSDELAIAWNILTPILNEIKKNNIKPELYEFGGRGPIGA
Sbjct: 565 VPDSYEHLLLDVIDGDSHLFMRSDELAIAWNILTPILNEIKKNNIKPELYEFGGRGPIGA 624

Query: 262 YYLWAKHGVRWVEE 276
           YYLWAKHGVRWVEE
Sbjct: 625 YYLWAKHGVRWVEE 638

BLAST of Lsi11G006580 vs. NCBI nr
Match: KAA0048116.1 (glucose-6-phosphate 1-dehydrogenase 4 [Cucumis melo var. makuwa])

HSP 1 Score: 519.6 bits (1337), Expect = 1.6e-143
Identity = 252/262 (96.18%), Postives = 256/262 (97.71%), Query Frame = 0

Query: 15  SIQFLPC-RFLDGGGIIGDIVHSHILQTIALLAMEPPISLDGEAIRNEKVKLLRSIRPLE 74
           S+Q   C +FLDGGGIIGDIVHSHILQTIALLAMEPPISLDGEAIRNEKVKLLRSIRPLE
Sbjct: 370 SVQVHICIKFLDGGGIIGDIVHSHILQTIALLAMEPPISLDGEAIRNEKVKLLRSIRPLE 429

Query: 75  PSDVVLGQYKSSTNDKVDLSLDNLTPTYFAGALYIDNARWDGVPFLIKSGLGLIKHCVEI 134
           PSDVVLGQYKSST+DKVDLSLDNLTPTYFAGALYIDNARWDGVPFLIKSGLGLIKHCVEI
Sbjct: 430 PSDVVLGQYKSSTDDKVDLSLDNLTPTYFAGALYIDNARWDGVPFLIKSGLGLIKHCVEI 489

Query: 135 RIQFRQVPGNIYREHIGYNNESATNEIILRDAPEEAILVRVNNKVPGLGLRLDSPELNLL 194
           RIQFRQVPGNIYREH GYNNESATNEIILRD PEEAILVRVNNK+PGLGLRLDSPELNLL
Sbjct: 490 RIQFRQVPGNIYREHFGYNNESATNEIILRDVPEEAILVRVNNKIPGLGLRLDSPELNLL 549

Query: 195 YKDKYNVDVPDSYEHLLLDVIDGDSHLFMRSDELAIAWNILTPILNEIKKNNIKPELYEF 254
           YKDKYNVDVPDSYEHLLLDVIDGDSHLFMRSDELAIAWNILTPILNEIKKNNIKPELYEF
Sbjct: 550 YKDKYNVDVPDSYEHLLLDVIDGDSHLFMRSDELAIAWNILTPILNEIKKNNIKPELYEF 609

Query: 255 GGRGPIGAYYLWAKHGVRWVEE 276
           GGRGPIGAYYLWAKHGVRWVEE
Sbjct: 610 GGRGPIGAYYLWAKHGVRWVEE 631

BLAST of Lsi11G006580 vs. NCBI nr
Match: XP_038891033.1 (inactive glucose-6-phosphate 1-dehydrogenase 4, chloroplastic [Benincasa hispida])

HSP 1 Score: 519.6 bits (1337), Expect = 1.6e-143
Identity = 251/254 (98.82%), Postives = 252/254 (99.21%), Query Frame = 0

Query: 22  RFLDGGGIIGDIVHSHILQTIALLAMEPPISLDGEAIRNEKVKLLRSIRPLEPSDVVLGQ 81
           RFLDGGGIIGDIVHSHILQTIALLAMEPPISLDGEAIRNEKVKLLRSIRPLE SDVVLGQ
Sbjct: 369 RFLDGGGIIGDIVHSHILQTIALLAMEPPISLDGEAIRNEKVKLLRSIRPLETSDVVLGQ 428

Query: 82  YKSSTNDKVDLSLDNLTPTYFAGALYIDNARWDGVPFLIKSGLGLIKHCVEIRIQFRQVP 141
           YKSSTNDKVDLSLDNLTPTYFAGALYIDNARWDGVPFLIKSGLGLIKHCVEIRIQFRQVP
Sbjct: 429 YKSSTNDKVDLSLDNLTPTYFAGALYIDNARWDGVPFLIKSGLGLIKHCVEIRIQFRQVP 488

Query: 142 GNIYREHIGYNNESATNEIILRDAPEEAILVRVNNKVPGLGLRLDSPELNLLYKDKYNVD 201
           GNIYREHIGYNNESATNEIILRD PEEAILVRVNNK+PGLGLRLDSPELNLLYKDKYNVD
Sbjct: 489 GNIYREHIGYNNESATNEIILRDVPEEAILVRVNNKIPGLGLRLDSPELNLLYKDKYNVD 548

Query: 202 VPDSYEHLLLDVIDGDSHLFMRSDELAIAWNILTPILNEIKKNNIKPELYEFGGRGPIGA 261
           VPDSYEHLLLDVIDGDSHLFMRSDELAIAWNILTPILNEIKKNNIKPELYEFGGRGPIGA
Sbjct: 549 VPDSYEHLLLDVIDGDSHLFMRSDELAIAWNILTPILNEIKKNNIKPELYEFGGRGPIGA 608

Query: 262 YYLWAKHGVRWVEE 276
           YYLWAKHGVRWVEE
Sbjct: 609 YYLWAKHGVRWVEE 622

BLAST of Lsi11G006580 vs. NCBI nr
Match: XP_008453203.1 (PREDICTED: glucose-6-phosphate 1-dehydrogenase 4, chloroplastic [Cucumis melo] >TYJ96436.1 glucose-6-phosphate 1-dehydrogenase 4 [Cucumis melo var. makuwa])

HSP 1 Score: 519.2 bits (1336), Expect = 2.1e-143
Identity = 250/254 (98.43%), Postives = 252/254 (99.21%), Query Frame = 0

Query: 22  RFLDGGGIIGDIVHSHILQTIALLAMEPPISLDGEAIRNEKVKLLRSIRPLEPSDVVLGQ 81
           RFLDGGGIIGDIVHSHILQTIALLAMEPPISLDGEAIRNEKVKLLRSIRPLEPSDVVLGQ
Sbjct: 385 RFLDGGGIIGDIVHSHILQTIALLAMEPPISLDGEAIRNEKVKLLRSIRPLEPSDVVLGQ 444

Query: 82  YKSSTNDKVDLSLDNLTPTYFAGALYIDNARWDGVPFLIKSGLGLIKHCVEIRIQFRQVP 141
           YKSST+DKVDLSLDNLTPTYFAGALYIDNARWDGVPFLIKSGLGLIKHCVEIRIQFRQVP
Sbjct: 445 YKSSTDDKVDLSLDNLTPTYFAGALYIDNARWDGVPFLIKSGLGLIKHCVEIRIQFRQVP 504

Query: 142 GNIYREHIGYNNESATNEIILRDAPEEAILVRVNNKVPGLGLRLDSPELNLLYKDKYNVD 201
           GNIYREH GYNNESATNEIILRD PEEAILVRVNNK+PGLGLRLDSPELNLLYKDKYNVD
Sbjct: 505 GNIYREHFGYNNESATNEIILRDVPEEAILVRVNNKIPGLGLRLDSPELNLLYKDKYNVD 564

Query: 202 VPDSYEHLLLDVIDGDSHLFMRSDELAIAWNILTPILNEIKKNNIKPELYEFGGRGPIGA 261
           VPDSYEHLLLDVIDGDSHLFMRSDELAIAWNILTPILNEIKKNNIKPELYEFGGRGPIGA
Sbjct: 565 VPDSYEHLLLDVIDGDSHLFMRSDELAIAWNILTPILNEIKKNNIKPELYEFGGRGPIGA 624

Query: 262 YYLWAKHGVRWVEE 276
           YYLWAKHGVRWVEE
Sbjct: 625 YYLWAKHGVRWVEE 638

BLAST of Lsi11G006580 vs. TAIR 10
Match: AT1G09420.1 (glucose-6-phosphate dehydrogenase 4 )

HSP 1 Score: 399.1 bits (1024), Expect = 3.0e-111
Identity = 185/254 (72.83%), Postives = 218/254 (85.83%), Query Frame = 0

Query: 22  RFLDGGGIIGDIVHSHILQTIALLAMEPPISLDGEAIRNEKVKLLRSIRPLEPSDVVLGQ 81
           +F DG GII DIVHSHILQTIALLAMEPPISLDGE IRNEKVK+LRSIR ++P DV+LGQ
Sbjct: 372 KFSDGYGIIRDIVHSHILQTIALLAMEPPISLDGEDIRNEKVKVLRSIRKIDPRDVILGQ 431

Query: 82  YKSSTNDKVDLSLDNLTPTYFAGALYIDNARWDGVPFLIKSGLGLIKHCVEIRIQFRQVP 141
           YKSS+ DK  + L+ + PTY A ALYIDNARWDGVPFL++ G GLIKH VEI +QFR VP
Sbjct: 432 YKSSSRDKNGVILNGVDPTYCAAALYIDNARWDGVPFLVRVGTGLIKHRVEIHVQFRHVP 491

Query: 142 GNIYREHIGYNNESATNEIILRDAPEEAILVRVNNKVPGLGLRLDSPELNLLYKDKYNVD 201
           GN+YRE+IG N +  TNE+ILRD P+EAILV++NNKVPGLGL+LD+ ELNLLYKD+Y  +
Sbjct: 492 GNLYRENIGINIDLGTNELILRDEPDEAILVKINNKVPGLGLQLDASELNLLYKDRYKTE 551

Query: 202 VPDSYEHLLLDVIDGDSHLFMRSDELAIAWNILTPILNEIKKNNIKPELYEFGGRGPIGA 261
           VPDSYEHL+ DVIDGD+HLFMRSDE+A AWNIL+P+L EI K++  PELYEFGGRGP+ A
Sbjct: 552 VPDSYEHLIHDVIDGDNHLFMRSDEVAAAWNILSPVLEEIDKHHTAPELYEFGGRGPVAA 611

Query: 262 YYLWAKHGVRWVEE 276
           YYLWAKHGV W ++
Sbjct: 612 YYLWAKHGVPWADD 625

BLAST of Lsi11G006580 vs. TAIR 10
Match: AT1G09420.2 (glucose-6-phosphate dehydrogenase 4 )

HSP 1 Score: 390.2 bits (1001), Expect = 1.4e-108
Identity = 185/264 (70.08%), Postives = 217/264 (82.20%), Query Frame = 0

Query: 22  RFLDGGGIIGDIVHSHILQTIALLAMEPPISLDGEAIRNEKVKL----------LRSIRP 81
           +F DG GII DIVHSHILQTIALLAMEPPISLDGE IRNEKV L          LRSIR 
Sbjct: 372 KFSDGYGIIRDIVHSHILQTIALLAMEPPISLDGEDIRNEKVNLYCKEQNRLMVLRSIRK 431

Query: 82  LEPSDVVLGQYKSSTNDKVDLSLDNLTPTYFAGALYIDNARWDGVPFLIKSGLGLIKHCV 141
           ++P DV+LGQYKSS+ DK  + L+ + PTY A ALYIDNARWDGVPFL++ G GLIKH V
Sbjct: 432 IDPRDVILGQYKSSSRDKNGVILNGVDPTYCAAALYIDNARWDGVPFLVRVGTGLIKHRV 491

Query: 142 EIRIQFRQVPGNIYREHIGYNNESATNEIILRDAPEEAILVRVNNKVPGLGLRLDSPELN 201
           EI +QFR VPGN+YRE+IG N +  TNE+ILRD P+EAILV++NNKVPGLGL+LD+ ELN
Sbjct: 492 EIHVQFRHVPGNLYRENIGINIDLGTNELILRDEPDEAILVKINNKVPGLGLQLDASELN 551

Query: 202 LLYKDKYNVDVPDSYEHLLLDVIDGDSHLFMRSDELAIAWNILTPILNEIKKNNIKPELY 261
           LLYKD+Y  +VPDSYEHL+ DVIDGD+HLFMRSDE+A AWNIL+P+L EI K++  PELY
Sbjct: 552 LLYKDRYKTEVPDSYEHLIHDVIDGDNHLFMRSDEVAAAWNILSPVLEEIDKHHTAPELY 611

Query: 262 EFGGRGPIGAYYLWAKHGVRWVEE 276
           EFGGRGP+ AYYLWAKHGV W ++
Sbjct: 612 EFGGRGPVAAYYLWAKHGVPWADD 635

BLAST of Lsi11G006580 vs. TAIR 10
Match: AT5G13110.1 (glucose-6-phosphate dehydrogenase 2 )

HSP 1 Score: 300.4 bits (768), Expect = 1.4e-81
Identity = 141/259 (54.44%), Postives = 187/259 (72.20%), Query Frame = 0

Query: 23  FLDGGGIIGDIVHSHILQTIALLAMEPPISLDGEAIRNEKVKLLRSIRPLEPSDVVLGQY 82
           + D  GII DI+ +H+LQ +AL AME P+SLD E IRNEKVK+LRS+RP+   DVV+GQY
Sbjct: 331 YFDNYGIIRDIMQNHLLQILALFAMETPVSLDAEDIRNEKVKVLRSMRPIRVEDVVIGQY 390

Query: 83  KSST---------NDKVDLSLDNLTPTYFAGALYIDNARWDGVPFLIKSGLGLIKHCVEI 142
           KS T          D   +   +LTPT+ A AL+IDNARWDGVPFL+K+G  L     EI
Sbjct: 391 KSHTKGGVTYPAYTDDKTVPKGSLTPTFAAAALFIDNARWDGVPFLMKAGKALHTRSAEI 450

Query: 143 RIQFRQVPGNIYREHIGYNNESATNEIILRDAPEEAILVRVNNKVPGLGLRLDSPELNLL 202
           R+QFR VPGN+Y  + G + + ATNE+++R  P+EAI +++NNKVPGLG+RLD   LNLL
Sbjct: 451 RVQFRHVPGNLYNRNTGSDLDQATNELVIRVQPDEAIYLKINNKVPGLGMRLDRSNLNLL 510

Query: 203 YKDKYNVDVPDSYEHLLLDVIDGDSHLFMRSDELAIAWNILTPILNEIKKNNIKPELYEF 262
           Y  +Y+ ++PD+YE LLLD I+G+  LF+RSDEL  AW++ TP+L EI++    PE Y +
Sbjct: 511 YSARYSKEIPDAYERLLLDAIEGERRLFIRSDELDAAWSLFTPLLKEIEEKKRIPEYYPY 570

Query: 263 GGRGPIGAYYLWAKHGVRW 273
           G RGP+GA+YL AKH V+W
Sbjct: 571 GSRGPVGAHYLAAKHKVQW 589

BLAST of Lsi11G006580 vs. TAIR 10
Match: AT1G24280.1 (glucose-6-phosphate dehydrogenase 3 )

HSP 1 Score: 300.1 bits (767), Expect = 1.9e-81
Identity = 140/259 (54.05%), Postives = 186/259 (71.81%), Query Frame = 0

Query: 23  FLDGGGIIGDIVHSHILQTIALLAMEPPISLDGEAIRNEKVKLLRSIRPLEPSDVVLGQY 82
           + D  GII DI+ +H+LQ +AL AME P+SLD E IRNEKVK+LRS+RP++  DVV+GQY
Sbjct: 334 YFDNYGIIRDIMQNHLLQILALFAMETPVSLDAEDIRNEKVKVLRSMRPIKLEDVVIGQY 393

Query: 83  KS---------STNDKVDLSLDNLTPTYFAGALYIDNARWDGVPFLIKSGLGLIKHCVEI 142
           KS         S  D   +   +LTPT+ A AL+IDNARWDGVPFL+K+G  L     EI
Sbjct: 394 KSHSIGGVTYPSYTDDKTVPKGSLTPTFAAAALFIDNARWDGVPFLMKAGKALNTRSAEI 453

Query: 143 RIQFRQVPGNIYREHIGYNNESATNEIILRDAPEEAILVRVNNKVPGLGLRLDSPELNLL 202
           R+QFR VPGN+Y  + G + +  TNE+++R  P+EAI +++NNKVPGLG+RLD   LNLL
Sbjct: 454 RVQFRHVPGNLYNRNSGTDRDQTTNELVIRVQPDEAIYLKINNKVPGLGMRLDQSNLNLL 513

Query: 203 YKDKYNVDVPDSYEHLLLDVIDGDSHLFMRSDELAIAWNILTPILNEIKKNNIKPELYEF 262
           Y  +Y+ ++PD+YE LLLD I+G+  LF+RSDEL  AW + TP+L EI++    PE Y +
Sbjct: 514 YSARYSKEIPDAYERLLLDAIEGERRLFIRSDELDAAWALFTPLLKEIEEKKTTPEFYPY 573

Query: 263 GGRGPIGAYYLWAKHGVRW 273
           G RGP+GA+YL AKH V+W
Sbjct: 574 GSRGPVGAHYLAAKHKVQW 592

BLAST of Lsi11G006580 vs. TAIR 10
Match: AT5G35790.1 (glucose-6-phosphate dehydrogenase 1 )

HSP 1 Score: 292.0 bits (746), Expect = 5.1e-79
Identity = 136/259 (52.51%), Postives = 187/259 (72.20%), Query Frame = 0

Query: 23  FLDGGGIIGDIVHSHILQTIALLAMEPPISLDGEAIRNEKVKLLRSIRPLEPSDVVLGQY 82
           + D  GII DI+ +H+LQ +AL AME P+SLD E IR+EKVK+LRS++PL   DVV+GQY
Sbjct: 312 YFDQYGIIRDIMQNHLLQILALFAMETPVSLDAEDIRSEKVKVLRSMKPLRLEDVVVGQY 371

Query: 83  KSST---------NDKVDLSLDNLTPTYFAGALYIDNARWDGVPFLIKSGLGLIKHCVEI 142
           K             D   +   +LTPT+ A A++I+NARWDGVPFL+K+G  L     EI
Sbjct: 372 KGHNKGGKTYPGYTDDPTVPNHSLTPTFAAAAMFINNARWDGVPFLMKAGKALHTRGAEI 431

Query: 143 RIQFRQVPGNIYREHIGYNNESATNEIILRDAPEEAILVRVNNKVPGLGLRLDSPELNLL 202
           R+QFR VPGN+Y++    N ++ATNE+++R  P+E I +R+NNKVPGLG+RLD  +LNLL
Sbjct: 432 RVQFRHVPGNLYKKSFATNLDNATNELVIRVQPDEGIYLRINNKVPGLGMRLDRSDLNLL 491

Query: 203 YKDKYNVDVPDSYEHLLLDVIDGDSHLFMRSDELAIAWNILTPILNEIKKNNIKPELYEF 262
           Y+ +Y  ++PD+YE LLLD I+G+  LF+RSDEL  AW++ TP L E+++  I PELY +
Sbjct: 492 YRSRYPREIPDAYERLLLDAIEGERRLFIRSDELDAAWDLFTPALKELEEKKIIPELYPY 551

Query: 263 GGRGPIGAYYLWAKHGVRW 273
           G RGP+GA+YL +K+ VRW
Sbjct: 552 GSRGPVGAHYLASKYNVRW 570

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q93ZW04.2e-11072.83Inactive glucose-6-phosphate 1-dehydrogenase 4, chloroplastic OS=Arabidopsis tha... [more]
Q438394.4e-8356.37Glucose-6-phosphate 1-dehydrogenase, chloroplastic OS=Solanum tuberosum OX=4113 ... [more]
Q437939.1e-8154.44Glucose-6-phosphate 1-dehydrogenase, chloroplastic OS=Nicotiana tabacum OX=4097 ... [more]
Q9FY992.0e-8054.44Glucose-6-phosphate 1-dehydrogenase 2, chloroplastic OS=Arabidopsis thaliana OX=... [more]
Q8L7432.6e-8054.05Glucose-6-phosphate 1-dehydrogenase 3, chloroplastic OS=Arabidopsis thaliana OX=... [more]
Match NameE-valueIdentityDescription
A0A0A0LH103.5e-14498.82Glucose-6-phosphate 1-dehydrogenase OS=Cucumis sativus OX=3659 GN=Csa_2G034760 P... [more]
A0A5A7U1K27.8e-14496.18Glucose-6-phosphate 1-dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6... [more]
A0A5D3BC051.0e-14398.43Glucose-6-phosphate 1-dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
A0A1S3BVM71.0e-14398.43Glucose-6-phosphate 1-dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103493988 PE=3... [more]
A0A6J1KGT25.3e-14096.06Glucose-6-phosphate 1-dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111493753 ... [more]
Match NameE-valueIdentityDescription
KAE8651631.17.3e-14498.82hypothetical protein Csa_021325 [Cucumis sativus][more]
XP_004144740.17.3e-14498.82inactive glucose-6-phosphate 1-dehydrogenase 4, chloroplastic [Cucumis sativus][more]
KAA0048116.11.6e-14396.18glucose-6-phosphate 1-dehydrogenase 4 [Cucumis melo var. makuwa][more]
XP_038891033.11.6e-14398.82inactive glucose-6-phosphate 1-dehydrogenase 4, chloroplastic [Benincasa hispida... [more]
XP_008453203.12.1e-14398.43PREDICTED: glucose-6-phosphate 1-dehydrogenase 4, chloroplastic [Cucumis melo] >... [more]
Match NameE-valueIdentityDescription
AT1G09420.13.0e-11172.83glucose-6-phosphate dehydrogenase 4 [more]
AT1G09420.21.4e-10870.08glucose-6-phosphate dehydrogenase 4 [more]
AT5G13110.11.4e-8154.44glucose-6-phosphate dehydrogenase 2 [more]
AT1G24280.11.9e-8154.05glucose-6-phosphate dehydrogenase 3 [more]
AT5G35790.15.1e-7952.51glucose-6-phosphate dehydrogenase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001282Glucose-6-phosphate dehydrogenasePRINTSPR00079G6PDHDRGNASEcoord: 34..50
score: 49.12
coord: 112..138
score: 49.97
IPR001282Glucose-6-phosphate dehydrogenasePANTHERPTHR23429GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE G6PDcoord: 21..275
NoneNo IPR availableGENE3D3.30.360.10Dihydrodipicolinate Reductase; domain 2coord: 21..274
e-value: 3.5E-77
score: 261.4
NoneNo IPR availablePANTHERPTHR23429:SF4INACTIVE GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE 4, CHLOROPLASTICcoord: 21..275
NoneNo IPR availableSUPERFAMILY55347Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domaincoord: 22..273
IPR022675Glucose-6-phosphate dehydrogenase, C-terminalPFAMPF02781G6PD_Ccoord: 22..272
e-value: 7.7E-73
score: 245.2

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi11G006580.1Lsi11G006580.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006006 glucose metabolic process
biological_process GO:0006098 pentose-phosphate shunt
molecular_function GO:0004345 glucose-6-phosphate dehydrogenase activity
molecular_function GO:0050661 NADP binding
molecular_function GO:0016614 oxidoreductase activity, acting on CH-OH group of donors