Lsi11G006250 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi11G006250
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Pseudouridine synthase
Location	chr11: 6640937 .. 6643464 (-)
RNA-Seq Expression	Lsi11G006250
Synteny	Lsi11G006250

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTTAAAATTGTTTACTCAGTTTTAGTCCTGAGGACCGAAGTTTTGCTAATTACATAAATGGCCCTCTTCATCGCACTTCAGCCCACTTTCTCAATTCCCTACAAAACCTTCACCAAATTCCCATTCCCAAAACCCTTCTGTTTCCATAGATTCAAATCTGTCAGCAATAGCATGGCGGACTCATCGGAACCGGTGACCGGAAACTGCCCATCAACCAGAGAGAATTACCCGGTTCCCTTGTCGCCTCCACTGCCGGCCATATCCAAGAATTTGGAGCTCGCTAGAGCCATGGCTGCCTCCTCTAAATCCAGCCTCTTTGCTTTATCGGCAAACGATGTTATATACGAGGACGAGTGGCTTATTGCCGTCAATAAGCCTCAAGGAATCTACTGTGAAAGCGTTTTGGCCGCTGTTCCTCGTCTTCTCGGTGATTCGGCTATGGCTGGTTTGTGGATCTTACTGTGAAAGCGTTTTTCTTTTCAGGCGAATTTGAATTTTAACTTGAACTTGGCAAGATGAATTCAATTTTACCCTATATTGTGAATTTGGTAATCGAATGGAAATGGTGGAATTGCAAGTTTTAACTTGCTCTTGTTTGAAGATTTGTAAAAAGGTATAATCGTGTGCTTTCCATGGATTACAAAATGTAGACGAAGTTGCAAAGGAAGAAGATATTAGGGCTGAATTTTTGTTATAATTGACGATTTTTGAAACATTTCTTCCATATAGATCGTGTGCATTGATTTTCTAGATCATATTTTTGTGCATAGATATGAACTTGGTTTTTGTTGAAGTTAGCTGGAATTAGTGGGTAAATGTGATGGAATTTTAACAGAAGTTTCAAAATTGTAATGCTTGAAGGCTATAGATTACCTCATCAAATTATATTGTATTGAAGTTTTCTGGGGTGCTCTGTGATATATAGATATCATGATAGGTGGGGATGCTCTGTTAGTTTGATATGAATACATTTACTTAATAAGCAAGAGCGAGTAATTGATATCTGATTCTTTCCCTGCATTGCCAAAACTAGCAATGTTGCTGATCGTTAGACAGTAAATATAAATAGTTTAGATGTATCCTGGTTGTTGAAGAGAGAGGTGTTTGAATTTGTCTGAAGTGTTTTGACGTTTGTCTAAAACTAAAAAAACAATGCCATGTAATTACATCTTTTGCTTATCCTATCATTATCGATTTCTCGGGAGTGTTCTTAGCTCAGATTCTTCATATGCAGGGATCAAAACCAGTCTTCCAGAGCTTCATCTTGCTAACCGCCTTGATCGTGACACGAGTGGGGTTATGGTTATAACTAAGTCACATAAAGTTGCTTCTAAATTAGTGAAGGCATTTACTGACCACACGGTTTCAAAATCATATATTGCCTTCTGTGTTGGCTCTGCTCCAAAATGGAAAAAAATCAACGTCAAATCTGGTCATGGAAGGTCAAAGTTTGGGGTCTGGCGTGTCTATGCTGCAGCTGACGTGGGTCGCTCCTTACCTGGCGGTTCCATAGTCAGAGACATGGAAACATATTTTGAAGTACTGTCTATAAAGGGAAAAAATGCCATGGAGGATTGTAAAAAATTTAGGAAAGATGAAGAAGAAACTATTGTAGTCCAAATAAAATCTCTGGTGGACATTGATGGTCAGAAGGACGAGATCTTGATAAGAGCACGACCTCGCAGTGGAAGAACTCATCAAATCCGTCTGCACTGCCAATATCTCGGAATTCCTATAAGAGGGGATGTGAAATATGAGGGTGTTACAGAGTGGAATGGAAAAAATTACGATAGCCATGAGCTTCATGCTGAAAGCTTGTATTTTGTGCACCCTGTTACAGGTATTCCTCTCAAACTACAAGCTCCTCTACCATCATGGGCCAGCCAAGCATTGCAGCCCCAACAACAAGAAGTGAATTCTCCACCATCCTTCAAACCTATGCCTTGACAAGACCATTGGCACAGTTAAGTGAGAAGAGAACCAAATATATCTCTTATTATACAAAGAGCTTCTGTATTGAAGAATGTCGCTAAAGCAAACTGGAGCGATCTTTGATCAAATTCGTACAAGTGTGACTGTGGACAGTTTTTGCACCATCAGTTTCCTGTCTGCAATCTCTCATCTATTGAATGAGCTCTTGTTAGCACAACAGTTCTACGAAAATATCATCCGAGTCGTAAACTGATCAGTCATCCGAGTTTTTCCTGAAAATTTGAGAGGATGAGATGCTCACAATGGACTCTGGAAATGTCAGAAGAATCATGTACGTCTAAGGCTGATAAATTTGTGCAAAGCCCTTTGGAAATTGATCTTCTGCCGCGACTTTATCGATAAAATTGATGTACAATGTTGTGGCAGCCAAATTATATGGTTAGCGTGTTGATTTATGTTGAGGTTCAATTGTTATTCGACTCCCCTTCCCTCCATTTGTGCAACTCTGTGTTCAAAATATGAAGAACAATTTTGTTATGATGTTTACGCGGCTATAAACTATAAATAGAAGAATGAGAAAACATGTTGAGTTTTT

mRNA sequence

TTTTAAAATTGTTTACTCAGTTTTAGTCCTGAGGACCGAAGTTTTGCTAATTACATAAATGGCCCTCTTCATCGCACTTCAGCCCACTTTCTCAATTCCCTACAAAACCTTCACCAAATTCCCATTCCCAAAACCCTTCTGTTTCCATAGATTCAAATCTGTCAGCAATAGCATGGCGGACTCATCGGAACCGGTGACCGGAAACTGCCCATCAACCAGAGAGAATTACCCGGTTCCCTTGTCGCCTCCACTGCCGGCCATATCCAAGAATTTGGAGCTCGCTAGAGCCATGGCTGCCTCCTCTAAATCCAGCCTCTTTGCTTTATCGGCAAACGATGTTATATACGAGGACGAGTGGCTTATTGCCGTCAATAAGCCTCAAGGAATCTACTGTGAAAGCGTTTTGGCCGCTGTTCCTCGTCTTCTCGGTGATTCGGCTATGGCTGGGATCAAAACCAGTCTTCCAGAGCTTCATCTTGCTAACCGCCTTGATCGTGACACGAGTGGGGTTATGGTTATAACTAAGTCACATAAAGTTGCTTCTAAATTAGTGAAGGCATTTACTGACCACACGGTTTCAAAATCATATATTGCCTTCTGTGTTGGCTCTGCTCCAAAATGGAAAAAAATCAACGTCAAATCTGGTCATGGAAGGTCAAAGTTTGGGGTCTGGCGTGTCTATGCTGCAGCTGACGTGGGTCGCTCCTTACCTGGCGGTTCCATAGTCAGAGACATGGAAACATATTTTGAAGTACTGTCTATAAAGGGAAAAAATGCCATGGAGGATTGTAAAAAATTTAGGAAAGATGAAGAAGAAACTATTGTAGTCCAAATAAAATCTCTGGTGGACATTGATGGTCAGAAGGACGAGATCTTGATAAGAGCACGACCTCGCAGTGGAAGAACTCATCAAATCCGTCTGCACTGCCAATATCTCGGAATTCCTATAAGAGGGGATGTGAAATATGAGGGTGTTACAGAGTGGAATGGAAAAAATTACGATAGCCATGAGCTTCATGCTGAAAGCTTGTATTTTGTGCACCCTGTTACAGGTATTCCTCTCAAACTACAAGCTCCTCTACCATCATGGGCCAGCCAAGCATTGCAGCCCCAACAACAAGAAGTGAATTCTCCACCATCCTTCAAACCTATGCCTTGACAAGACCATTGGCACAGTTAAGTGAGAAGAGAACCAAATATATCTCTTATTATACAAAGAGCTTCTGTATTGAAGAATGTCGCTAAAGCAAACTGGAGCGATCTTTGATCAAATTCGTACAAGTGTGACTGTGGACAGTTTTTGCACCATCAGTTTCCTGTCTGCAATCTCTCATCTATTGAATGAGCTCTTGTTAGCACAACAGTTCTACGAAAATATCATCCGAGTCGTAAACTGATCAGTCATCCGAGTTTTTCCTGAAAATTTGAGAGGATGAGATGCTCACAATGGACTCTGGAAATGTCAGAAGAATCATGTACGTCTAAGGCTGATAAATTTGTGCAAAGCCCTTTGGAAATTGATCTTCTGCCGCGACTTTATCGATAAAATTGATGTACAATGTTGTGGCAGCCAAATTATATGGTTAGCGTGTTGATTTATGTTGAGGTTCAATTGTTATTCGACTCCCCTTCCCTCCATTTGTGCAACTCTGTGTTCAAAATATGAAGAACAATTTTGTTATGATGTTTACGCGGCTATAAACTATAAATAGAAGAATGAGAAAACATGTTGAGTTTTT

Coding sequence (CDS)

ATGGCCCTCTTCATCGCACTTCAGCCCACTTTCTCAATTCCCTACAAAACCTTCACCAAATTCCCATTCCCAAAACCCTTCTGTTTCCATAGATTCAAATCTGTCAGCAATAGCATGGCGGACTCATCGGAACCGGTGACCGGAAACTGCCCATCAACCAGAGAGAATTACCCGGTTCCCTTGTCGCCTCCACTGCCGGCCATATCCAAGAATTTGGAGCTCGCTAGAGCCATGGCTGCCTCCTCTAAATCCAGCCTCTTTGCTTTATCGGCAAACGATGTTATATACGAGGACGAGTGGCTTATTGCCGTCAATAAGCCTCAAGGAATCTACTGTGAAAGCGTTTTGGCCGCTGTTCCTCGTCTTCTCGGTGATTCGGCTATGGCTGGGATCAAAACCAGTCTTCCAGAGCTTCATCTTGCTAACCGCCTTGATCGTGACACGAGTGGGGTTATGGTTATAACTAAGTCACATAAAGTTGCTTCTAAATTAGTGAAGGCATTTACTGACCACACGGTTTCAAAATCATATATTGCCTTCTGTGTTGGCTCTGCTCCAAAATGGAAAAAAATCAACGTCAAATCTGGTCATGGAAGGTCAAAGTTTGGGGTCTGGCGTGTCTATGCTGCAGCTGACGTGGGTCGCTCCTTACCTGGCGGTTCCATAGTCAGAGACATGGAAACATATTTTGAAGTACTGTCTATAAAGGGAAAAAATGCCATGGAGGATTGTAAAAAATTTAGGAAAGATGAAGAAGAAACTATTGTAGTCCAAATAAAATCTCTGGTGGACATTGATGGTCAGAAGGACGAGATCTTGATAAGAGCACGACCTCGCAGTGGAAGAACTCATCAAATCCGTCTGCACTGCCAATATCTCGGAATTCCTATAAGAGGGGATGTGAAATATGAGGGTGTTACAGAGTGGAATGGAAAAAATTACGATAGCCATGAGCTTCATGCTGAAAGCTTGTATTTTGTGCACCCTGTTACAGGTATTCCTCTCAAACTACAAGCTCCTCTACCATCATGGGCCAGCCAAGCATTGCAGCCCCAACAACAAGAAGTGAATTCTCCACCATCCTTCAAACCTATGCCTTGA

Protein sequence

MALFIALQPTFSIPYKTFTKFPFPKPFCFHRFKSVSNSMADSSEPVTGNCPSTRENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCESVLAAVPRLLGDSAMAGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDMETYFEVLSIKGKNAMEDCKKFRKDEEETIVVQIKSLVDIDGQKDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKNYDSHELHAESLYFVHPVTGIPLKLQAPLPSWASQALQPQQQEVNSPPSFKPMP

Homology

[Close]

BLAST of Lsi11G006250 vs. ExPASy Swiss-Prot
Match: Q2QNM3 (RNA pseudouridine synthase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g0560500 PE=2 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 2.3e-100
Identity = 181/295 (61.36%), Postives = 223/295 (75.59%), Query Frame = 0


Query: 57  YPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCESVL 116
           YPVP+SPP PA SK++EL RAM AS++S+  A S+  V++EDEWL  V+KP G+YC+++L
Sbjct: 51  YPVPVSPPYPAASKDVELRRAMTASARSA--AYSSAPVVFEDEWLAVVDKPAGVYCDALL 110

Query: 117 AAVPRLLGDSAMAGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKS 176
           +A+P     +A  G + + P LHLANRLDRDTSG+MVITK +KVA KLVKAFT+H V K+
Sbjct: 111 SALP---CSAATLGDEATKPNLHLANRLDRDTSGLMVITKCNKVAGKLVKAFTEHKVKKT 170

Query: 177 YIAFCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDMETYFEVLSIK 236
           Y+A C+G  P W+KI + SGHGRSK G WRVYA +DVGRSLPGGS+VRDM T FEVL I 
Sbjct: 171 YLALCIGYPPAWEKIKICSGHGRSKHGAWRVYAMSDVGRSLPGGSVVRDMSTRFEVLGIN 230

Query: 237 GKNAMEDCKKFRKDEEETIVVQIKSLVDIDGQKDE----ILIRARPRSGRTHQIRLHCQY 296
           GK    +   F  DE E+I VQ K+  D+    DE    IL+RA P+SGRTHQIRLHCQY
Sbjct: 231 GKGQFREPSNFEVDETESITVQEKA-ADLTSDGDEKNSIILVRAYPQSGRTHQIRLHCQY 290

Query: 297 LGIPIRGDVKYEGVTEWNGKNYDSHELHAESLYFVHPVTGIPLKLQAPLPSWASQ 348
           LG PIRGDVKY GV EWNG +YD H LHAESL FVHPVTG+P+  ++PLPSWA++
Sbjct: 291 LGFPIRGDVKYSGVIEWNGVDYDGHALHAESLSFVHPVTGLPVTFRSPLPSWANE 339

[Close]

BLAST of Lsi11G006250 vs. ExPASy Swiss-Prot
Match: Q7XA65 (RNA pseudouridine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g56345 PE=2 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 2.2e-98
Identity = 180/294 (61.22%), Postives = 224/294 (76.19%), Query Frame = 0


Query: 56  NYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCESV 115
           NYP P+S P P ISK++EL RAM ASSKSSLF L+ +D++YEDE+L+AVNKP+G+YCE+V
Sbjct: 39  NYPKPISAPPPPISKDIELRRAMEASSKSSLFNLTRDDILYEDEYLMAVNKPKGVYCEAV 98

Query: 116 LAAVPRLLGDSAMAGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSK 175
           L + P+++ DS+         E HLANRLDRDTSGVM+ITKSHKVA+KLVKAFT+H + K
Sbjct: 99  LRSAPQIVLDSS--------SEYHLANRLDRDTSGVMIITKSHKVAAKLVKAFTEHKIRK 158

Query: 176 SYIAFCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDMETYFEVLSI 235
           SYIA C+GS+P W+++ V SGHGRSK G WRVYAA DVGR LPGGS VRDMET FEV+S+
Sbjct: 159 SYIALCIGSSPNWRRVTVSSGHGRSKHGAWRVYAALDVGRVLPGGSFVRDMETTFEVVSV 218

Query: 236 KG-KNAMEDCKKFRKDEEETIVVQIKSLVDIDGQKDE--ILIRARPRSGRTHQIRLHCQY 295
              KN  E C+   +D    IV + +  +   G  D+  +++RA PRSGRTHQIRLHCQY
Sbjct: 219 NSVKN--ESCE--LEDVNHVIVAEGERELSCGGDDDDVVVVVRAFPRSGRTHQIRLHCQY 278

Query: 296 LGIPIRGDVKYEGVTEWNGKNYDSHELHAESLYFVHPVTGIPLKLQAPLPSWAS 347
           LGIPIRGDVKY GV EWNG+ ++ HELHAE L   HPVTG  + ++APLP WA+
Sbjct: 279 LGIPIRGDVKYHGVYEWNGRTFEGHELHAECLSLDHPVTGDSIVIRAPLPYWAA 320

[Close]

BLAST of Lsi11G006250 vs. ExPASy Swiss-Prot
Match: Q68XB2 (Ribosomal large subunit pseudouridine synthase C OS=Rickettsia typhi (strain ATCC VR-144 / Wilmington) OX=257363 GN=rluC PE=3 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 2.2e-18
Identity = 81/256 (31.64%), Postives = 117/256 (45.70%), Query Frame = 0


Query: 94  VIYEDEWLIAVNKPQGIYCESVLAAVPRLLGDSAMAGIKTSLPELHLANRLDRDTSGVMV 153
           +IYED+ LIA+NKP  +  +    +   L  DSA+  +     +  L +RLD++TSG+++
Sbjct: 91  LIYEDDHLIAINKPASLATQG--GSKINLSIDSALKYLNYQGADFKLVHRLDKETSGLLL 150

Query: 154 ITKSHKVASKLVKAFTDHTVSKSYIAFCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADV 213
           I K++  + KL  AF +  V K Y A   G   K   I VKS  G+SK    R++   D+
Sbjct: 151 IAKNYLSSVKLHDAFKEKLVIKKYFAITYGRPVKNVGI-VKSNIGKSK---GRMFKITDI 210

Query: 214 GRSLPGGSIVRDMETYFEVLSIKGKNAMEDCKKFRKDEEETIVVQIKSLVDIDGQKDEIL 273
                               S  GK A+            T    +KSL       +  L
Sbjct: 211 D-------------------SDNGKLAI------------TYYKLLKSL-----NNNLFL 270

Query: 274 IRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKNYDSHELHAESLYFVHPVTGI 333
           I   P +GR HQ+RLH Q LG PI GD KY          Y    LHA ++Y    + G 
Sbjct: 271 IEFMPVTGRMHQLRLHAQLLGCPILGDDKYGNKEVMPYSKY--MFLHANNIYLSESIVGK 302

Query: 334 PLKLQAPLPSWASQAL 350
            +KL+A LP + ++ L
Sbjct: 331 EIKLEAKLPFYFTRRL 302

[Close]

BLAST of Lsi11G006250 vs. ExPASy Swiss-Prot
Match: Q9ZDR7 (Ribosomal large subunit pseudouridine synthase C OS=Rickettsia prowazekii (strain Madrid E) OX=272947 GN=rluC PE=3 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 6.5e-18
Identity = 78/256 (30.47%), Postives = 114/256 (44.53%), Query Frame = 0


Query: 94  VIYEDEWLIAVNKPQGIYCESVLAAVPRLLGDSAMAGIKTSLPELHLANRLDRDTSGVMV 153
           +IYED+ LIA+NKP  +  +    +   L  DSA+  +     +  L +RLD++TSG+++
Sbjct: 91  LIYEDDHLIAINKPASLATQG--GSKINLSIDSALKYLNYQGADFKLVHRLDKETSGLLL 150

Query: 154 ITKSHKVASKLVKAFTDHTVSKSYIAFCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADV 213
           I K++  + KL  AF +  V K Y A   G  P      VKS  G+SK            
Sbjct: 151 IAKNYLSSVKLHNAFKEKLVIKKYFAITYG-RPVKNVGTVKSNIGKSK------------ 210

Query: 214 GRSLPGGSIVRDMETYFEVLSIKGKNAMEDCKKFRKDEEETIVVQIKSLVDIDGQKDEIL 273
                     R +     + S  GK A+            T    +KSL       +  L
Sbjct: 211 ----------RRLSKIANIDSDDGKLAI------------TYYKLLKSL-----NNNLFL 270

Query: 274 IRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKNYDSHELHAESLYFVHPVTGI 333
           I   P +GR HQ+RLH Q LG PI GD KY          Y    LHA ++Y    + G 
Sbjct: 271 IEFMPVTGRMHQLRLHAQLLGCPILGDDKYGNKEIMPYSKY--MFLHANNIYLSETIVGK 302

Query: 334 PLKLQAPLPSWASQAL 350
            +KL+A LP + ++ +
Sbjct: 331 EIKLEAKLPFYFTRRI 302

[Close]

BLAST of Lsi11G006250 vs. ExPASy Swiss-Prot
Match: Q87S65 (Ribosomal large subunit pseudouridine synthase D OS=Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633) OX=223926 GN=rluD PE=3 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 7.2e-17
Identity = 77/292 (26.37%), Postives = 124/292 (42.47%), Query Frame = 0


Query: 93  DVIYEDEWLIAVNKPQ--------GIYCESVLAAVPRLLGDSAMAGIKTSLPELHLANRL 152
           D++YED+ +I +NKP+        G    +VL A+     D A       +P   + +RL
Sbjct: 85  DIVYEDDDIIVINKPRDFVVHPGAGTPDGTVLNALLHHYPDIA------EVPRAGIVHRL 144

Query: 153 DRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGSAPKWKKINVKSGHGRSKFGV 212
           D+DT+G+MV+ K+    ++LV+A     +++ Y A  +G      K++   G   +K   
Sbjct: 145 DKDTTGLMVVAKTVPAQTRLVRALQKRNITREYEAIAIGRMTAGGKVDQPIGRHSTK--- 204

Query: 213 WRVYAAADVGRSLPGGSIVRDMETYFEVLSIKGKNAMEDCKKFRKDEEETIVVQIKSLVD 272
             + A A +G+  P  +  R  E +                                   
Sbjct: 205 RTLMAVAPLGK--PAVTHYRVAEHF----------------------------------- 264

Query: 273 IDGQKDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEG-----------VTEWNGKN 332
               ++   IR R  +GRTHQIR+H  YL  P+ GD  Y G           +T+   + 
Sbjct: 265 ----REHTRIRLRLETGRTHQIRVHMSYLQHPLLGDTAYGGRARIPTGASQELTDMI-RG 324

Query: 333 YDSHELHAESLYFVHPVTGIPLKLQAPLPS---WASQALQPQQQEVNSPPSF 363
           +D   LHA  L F HP+TG  L+  AP+P      ++AL+   +E   P  F
Sbjct: 325 FDRQALHAVMLRFEHPITGEELEFHAPVPDDMVAMTEALRKDTEEYGLPDEF 325

[Close]

BLAST of Lsi11G006250 vs. ExPASy TrEMBL
Match: A0A0A0LJY6 (PseudoU_synth_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G033960 PE=4 SV=1)

HSP 1 Score: 666.0 bits (1717), Expect = 9.0e-188
Identity = 332/366 (90.71%), Postives = 343/366 (93.72%), Query Frame = 0


Query: 1   MALFIALQPTFSIPYKTFTKFPFPKPFCFHRFKSVSNSMADSSEPVTGNCPSTRENYPVP 60
           MALFI+L P FSI  KTFT+FPF K FCFHRFKSVSNSM DSSEPVTGNCPST ENYPVP
Sbjct: 1   MALFISLHPNFSISPKTFTEFPFTKSFCFHRFKSVSNSMTDSSEPVTGNCPSTAENYPVP 60

Query: 61  LSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCESVLAAVP 120
           LSPPLPAISKNLELARAM ASSKSSL+ALSANDVIYEDEWLIAVNKPQGIYCE+VLAAVP
Sbjct: 61  LSPPLPAISKNLELARAMVASSKSSLYALSANDVIYEDEWLIAVNKPQGIYCENVLAAVP 120

Query: 121 RLLGDSAMAGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAF 180
           RLLGDSA AGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAF
Sbjct: 121 RLLGDSANAGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAF 180

Query: 181 CVGSAPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDMETYFEVLSIKGKNA 240
           CVG++PKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGS+VRDMETYFEVLS+ GKN 
Sbjct: 181 CVGTSPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGKNT 240

Query: 241 MEDCKKFRKDEEETIVVQIKSLVDIDGQKDEILIRARPRSGRTHQIRLHCQYLGIPIRGD 300
           ME+ +KFR+ EEETIVV  KSLVDID  KDEILIRARPRSGRTHQIRLHCQYLGIPIRGD
Sbjct: 241 MEELQKFRRGEEETIVVHTKSLVDIDSHKDEILIRARPRSGRTHQIRLHCQYLGIPIRGD 300

Query: 301 VKYEGVTEWNGKNYDSHELHAESLYFVHPVTGIPLKLQAPLPSWASQALQPQQQEVNSPP 360
           VKYEGVTEWN K YDSHELHAESLYFVHPVTGIPLKLQAPLPSWASQALQPQQ EVNSP 
Sbjct: 301 VKYEGVTEWNEKIYDSHELHAESLYFVHPVTGIPLKLQAPLPSWASQALQPQQHEVNSPQ 360

Query: 361 SFKPMP 367
           SFK MP
Sbjct: 361 SFKTMP 366

[Close]

BLAST of Lsi11G006250 vs. ExPASy TrEMBL
Match: A0A5A7TX25 (RNA pseudouridine synthase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G001190 PE=4 SV=1)

HSP 1 Score: 614.0 bits (1582), Expect = 4.1e-172
Identity = 304/328 (92.68%), Postives = 315/328 (96.04%), Query Frame = 0


Query: 39  MADSSEPVTGNCPSTRENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 98
           M DSSEPVTGNCPST ENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED
Sbjct: 1   MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 60

Query: 99  EWLIAVNKPQGIYCESVLAAVPRLLGDSAMAGIKTSLPELHLANRLDRDTSGVMVITKSH 158
           EWLIAVNKPQGIYCE+VLAAVPRLL DSA AGIKT+LPELHLANRLDRDTSGVMVITKSH
Sbjct: 61  EWLIAVNKPQGIYCENVLAAVPRLLCDSANAGIKTNLPELHLANRLDRDTSGVMVITKSH 120

Query: 159 KVASKLVKAFTDHTVSKSYIAFCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLP 218
           KVASKLVKAFTDHTVSKSYIAFCVG++PKWKKI+VKSGHGRSKFGVWRVYAAADVGRSLP
Sbjct: 121 KVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLP 180

Query: 219 GGSIVRDMETYFEVLSIKGKNAMEDCKKFRKDEEETIVVQIKSLVDIDGQKDEILIRARP 278
           GGSIVRDMETYFEVLS+ GKN ME+ +KFRK+EEETIVV  KSLVDID QKDE+LIRARP
Sbjct: 181 GGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARP 240

Query: 279 RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKNYDSHELHAESLYFVHPVTGIPLKLQ 338
           RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGK YDSHELHAESLYFVHP+TGIPLKLQ
Sbjct: 241 RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQ 300

Query: 339 APLPSWASQALQPQQQEVNSPPSFKPMP 367
           APLPSWASQALQPQQQEVNSP SFK MP
Sbjct: 301 APLPSWASQALQPQQQEVNSPQSFKTMP 328

[Close]

BLAST of Lsi11G006250 vs. ExPASy TrEMBL
Match: A0A1S3BW87 (RNA pseudouridine synthase 1 OS=Cucumis melo OX=3656 GN=LOC103494301 PE=4 SV=1)

HSP 1 Score: 614.0 bits (1582), Expect = 4.1e-172
Identity = 304/328 (92.68%), Postives = 315/328 (96.04%), Query Frame = 0


Query: 39  MADSSEPVTGNCPSTRENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 98
           M DSSEPVTGNCPST ENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED
Sbjct: 1   MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 60

Query: 99  EWLIAVNKPQGIYCESVLAAVPRLLGDSAMAGIKTSLPELHLANRLDRDTSGVMVITKSH 158
           EWLIAVNKPQGIYCE+VLAAVPRLL DSA AGIKT+LPELHLANRLDRDTSGVMVITKSH
Sbjct: 61  EWLIAVNKPQGIYCENVLAAVPRLLCDSANAGIKTNLPELHLANRLDRDTSGVMVITKSH 120

Query: 159 KVASKLVKAFTDHTVSKSYIAFCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLP 218
           KVASKLVKAFTDHTVSKSYIAFCVG++PKWKKI+VKSGHGRSKFGVWRVYAAADVGRSLP
Sbjct: 121 KVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLP 180

Query: 219 GGSIVRDMETYFEVLSIKGKNAMEDCKKFRKDEEETIVVQIKSLVDIDGQKDEILIRARP 278
           GGSIVRDMETYFEVLS+ GKN ME+ +KFRK+EEETIVV  KSLVDID QKDE+LIRARP
Sbjct: 181 GGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARP 240

Query: 279 RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKNYDSHELHAESLYFVHPVTGIPLKLQ 338
           RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGK YDSHELHAESLYFVHP+TGIPLKLQ
Sbjct: 241 RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQ 300

Query: 339 APLPSWASQALQPQQQEVNSPPSFKPMP 367
           APLPSWASQALQPQQQEVNSP SFK MP
Sbjct: 301 APLPSWASQALQPQQQEVNSPQSFKTMP 328

[Close]

BLAST of Lsi11G006250 vs. ExPASy TrEMBL
Match: A0A6J1DQ12 (RNA pseudouridine synthase 1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111023261 PE=4 SV=1)

HSP 1 Score: 569.7 bits (1467), Expect = 8.8e-159
Identity = 285/330 (86.36%), Postives = 303/330 (91.82%), Query Frame = 0


Query: 39  MADSSEPVTGNCPSTRENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 98
           MAD S PVTGN P+T +NYP+PLSPPLPAISKNLELARAMAASSKSSLFALSA DVI+ED
Sbjct: 1   MADPSIPVTGNSPATGQNYPIPLSPPLPAISKNLELARAMAASSKSSLFALSATDVIFED 60

Query: 99  EWLIAVNKPQGIYCESVLAAVPRLLGDSAMA-----GIKTSLPELHLANRLDRDTSGVMV 158
           EWLIAVNKPQG+YCESVLA+VPRLLGDSAMA     GI+ SLPELHLANRLDRDTSGVMV
Sbjct: 61  EWLIAVNKPQGVYCESVLASVPRLLGDSAMAGEQSGGIQISLPELHLANRLDRDTSGVMV 120

Query: 159 ITKSHKVASKLVKAFTDHTVSKSYIAFCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADV 218
           ITKSHKVASKLVKAFTDH VSKSYIAFCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADV
Sbjct: 121 ITKSHKVASKLVKAFTDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADV 180

Query: 219 GRSLPGGSIVRDMETYFEVLSIKGKNAMEDCKKFRKDEEETIVVQIKSLVDIDGQKDEIL 278
           GRSLPGGS+VRDMETYFEVLS+ G++ M++  + RKDEEETIVVQ KSL+DID  KDEIL
Sbjct: 181 GRSLPGGSVVRDMETYFEVLSVNGQSTMDELPEVRKDEEETIVVQSKSLIDIDAHKDEIL 240

Query: 279 IRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKNYDSHELHAESLYFVHPVTGI 338
           IRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGV EWNG  YDSHELHAESL+FVHPVTGI
Sbjct: 241 IRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVIEWNGNIYDSHELHAESLFFVHPVTGI 300

Query: 339 PLKLQAPLPSWASQALQPQQQEVNSPPSFK 364
           PLKLQAPLPSWASQAL  QQQEV+SP + K
Sbjct: 301 PLKLQAPLPSWASQALLSQQQEVSSPSTLK 330

[Close]

BLAST of Lsi11G006250 vs. ExPASy TrEMBL
Match: A0A6J1DT56 (RNA pseudouridine synthase 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111023261 PE=4 SV=1)

HSP 1 Score: 565.8 bits (1457), Expect = 1.3e-157
Identity = 285/340 (83.82%), Postives = 303/340 (89.12%), Query Frame = 0


Query: 39  MADSSEPVTGNCPSTRENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 98
           MAD S PVTGN P+T +NYP+PLSPPLPAISKNLELARAMAASSKSSLFALSA DVI+ED
Sbjct: 1   MADPSIPVTGNSPATGQNYPIPLSPPLPAISKNLELARAMAASSKSSLFALSATDVIFED 60

Query: 99  EWLIAVNKPQGIYCESVLAAVPRLLGDSAMAG---------------IKTSLPELHLANR 158
           EWLIAVNKPQG+YCESVLA+VPRLLGDSAMAG               I+ SLPELHLANR
Sbjct: 61  EWLIAVNKPQGVYCESVLASVPRLLGDSAMAGEQSGGTTTLCLCLARIQISLPELHLANR 120

Query: 159 LDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGSAPKWKKINVKSGHGRSKFG 218
           LDRDTSGVMVITKSHKVASKLVKAFTDH VSKSYIAFCVGSAPKWKKINVKSGHGRSKFG
Sbjct: 121 LDRDTSGVMVITKSHKVASKLVKAFTDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFG 180

Query: 219 VWRVYAAADVGRSLPGGSIVRDMETYFEVLSIKGKNAMEDCKKFRKDEEETIVVQIKSLV 278
           VWRVYAAADVGRSLPGGS+VRDMETYFEVLS+ G++ M++  + RKDEEETIVVQ KSL+
Sbjct: 181 VWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGQSTMDELPEVRKDEEETIVVQSKSLI 240

Query: 279 DIDGQKDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKNYDSHELHAES 338
           DID  KDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGV EWNG  YDSHELHAES
Sbjct: 241 DIDAHKDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVIEWNGNIYDSHELHAES 300

Query: 339 LYFVHPVTGIPLKLQAPLPSWASQALQPQQQEVNSPPSFK 364
           L+FVHPVTGIPLKLQAPLPSWASQAL  QQQEV+SP + K
Sbjct: 301 LFFVHPVTGIPLKLQAPLPSWASQALLSQQQEVSSPSTLK 340

[Close]

BLAST of Lsi11G006250 vs. NCBI nr
Match: XP_004144814.3 (RNA pseudouridine synthase 1 [Cucumis sativus] >KGN60992.1 hypothetical protein Csa_021313 [Cucumis sativus])

HSP 1 Score: 666.0 bits (1717), Expect = 1.9e-187
Identity = 332/366 (90.71%), Postives = 343/366 (93.72%), Query Frame = 0


Query: 1   MALFIALQPTFSIPYKTFTKFPFPKPFCFHRFKSVSNSMADSSEPVTGNCPSTRENYPVP 60
           MALFI+L P FSI  KTFT+FPF K FCFHRFKSVSNSM DSSEPVTGNCPST ENYPVP
Sbjct: 1   MALFISLHPNFSISPKTFTEFPFTKSFCFHRFKSVSNSMTDSSEPVTGNCPSTAENYPVP 60

Query: 61  LSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCESVLAAVP 120
           LSPPLPAISKNLELARAM ASSKSSL+ALSANDVIYEDEWLIAVNKPQGIYCE+VLAAVP
Sbjct: 61  LSPPLPAISKNLELARAMVASSKSSLYALSANDVIYEDEWLIAVNKPQGIYCENVLAAVP 120

Query: 121 RLLGDSAMAGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAF 180
           RLLGDSA AGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAF
Sbjct: 121 RLLGDSANAGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAF 180

Query: 181 CVGSAPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDMETYFEVLSIKGKNA 240
           CVG++PKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGS+VRDMETYFEVLS+ GKN 
Sbjct: 181 CVGTSPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGKNT 240

Query: 241 MEDCKKFRKDEEETIVVQIKSLVDIDGQKDEILIRARPRSGRTHQIRLHCQYLGIPIRGD 300
           ME+ +KFR+ EEETIVV  KSLVDID  KDEILIRARPRSGRTHQIRLHCQYLGIPIRGD
Sbjct: 241 MEELQKFRRGEEETIVVHTKSLVDIDSHKDEILIRARPRSGRTHQIRLHCQYLGIPIRGD 300

Query: 301 VKYEGVTEWNGKNYDSHELHAESLYFVHPVTGIPLKLQAPLPSWASQALQPQQQEVNSPP 360
           VKYEGVTEWN K YDSHELHAESLYFVHPVTGIPLKLQAPLPSWASQALQPQQ EVNSP 
Sbjct: 301 VKYEGVTEWNEKIYDSHELHAESLYFVHPVTGIPLKLQAPLPSWASQALQPQQHEVNSPQ 360

Query: 361 SFKPMP 367
           SFK MP
Sbjct: 361 SFKTMP 366

[Close]

BLAST of Lsi11G006250 vs. NCBI nr
Match: XP_038889468.1 (RNA pseudouridine synthase 1 [Benincasa hispida])

HSP 1 Score: 660.6 bits (1703), Expect = 7.8e-186
Identity = 332/359 (92.48%), Postives = 343/359 (95.54%), Query Frame = 0


Query: 1   MALFIALQPTFSIPYKTFTKFPFPKPFCFHRFKSVSNSMADSSEPVTGNCPSTRENYPVP 60
           MAL I+LQPTFS P+KTFT+  FPKPFC  RFKS+S SMADSSEPVTGNCPSTRENYPVP
Sbjct: 1   MALLISLQPTFSTPHKTFTE--FPKPFCSGRFKSISKSMADSSEPVTGNCPSTRENYPVP 60

Query: 61  LSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCESVLAAVP 120
           LSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCESVLAAVP
Sbjct: 61  LSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCESVLAAVP 120

Query: 121 RLLGDSAMAGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAF 180
           RLL DSAMAGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAF
Sbjct: 121 RLLSDSAMAGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAF 180

Query: 181 CVGSAPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDMETYFEVLSIKGKNA 240
           CVGSAPKWKKIN+KSGHGRSKFGVWRVYAAADVGRSLPGGS+VRDMETYFEVLSI G+NA
Sbjct: 181 CVGSAPKWKKINIKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSINGQNA 240

Query: 241 MEDCKKFRKDEEETIVVQIKSLVDIDGQKDEILIRARPRSGRTHQIRLHCQYLGIPIRGD 300
           ME+ +KF KD EET+VVQ KSLVDIDGQKDEILIRARPRSGRTHQIRLHCQYLGIPIRGD
Sbjct: 241 MEEFQKFGKD-EETVVVQTKSLVDIDGQKDEILIRARPRSGRTHQIRLHCQYLGIPIRGD 300

Query: 301 VKYEGVTEWNGKNYDSHELHAESLYFVHPVTGIPLKLQAPLPSWASQALQPQQQEVNSP 360
           VKYEGVTEWNGK YD HELHAESLYFVHP+TGIPLKLQAPLPSWASQA QPQQQEVNSP
Sbjct: 301 VKYEGVTEWNGKIYDCHELHAESLYFVHPITGIPLKLQAPLPSWASQAWQPQQQEVNSP 356

[Close]

BLAST of Lsi11G006250 vs. NCBI nr
Match: XP_008453651.1 (PREDICTED: RNA pseudouridine synthase 1 [Cucumis melo] >KAA0048062.1 RNA pseudouridine synthase 1 [Cucumis melo var. makuwa] >TYJ96474.1 RNA pseudouridine synthase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 614.0 bits (1582), Expect = 8.4e-172
Identity = 304/328 (92.68%), Postives = 315/328 (96.04%), Query Frame = 0


Query: 39  MADSSEPVTGNCPSTRENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 98
           M DSSEPVTGNCPST ENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED
Sbjct: 1   MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 60

Query: 99  EWLIAVNKPQGIYCESVLAAVPRLLGDSAMAGIKTSLPELHLANRLDRDTSGVMVITKSH 158
           EWLIAVNKPQGIYCE+VLAAVPRLL DSA AGIKT+LPELHLANRLDRDTSGVMVITKSH
Sbjct: 61  EWLIAVNKPQGIYCENVLAAVPRLLCDSANAGIKTNLPELHLANRLDRDTSGVMVITKSH 120

Query: 159 KVASKLVKAFTDHTVSKSYIAFCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLP 218
           KVASKLVKAFTDHTVSKSYIAFCVG++PKWKKI+VKSGHGRSKFGVWRVYAAADVGRSLP
Sbjct: 121 KVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLP 180

Query: 219 GGSIVRDMETYFEVLSIKGKNAMEDCKKFRKDEEETIVVQIKSLVDIDGQKDEILIRARP 278
           GGSIVRDMETYFEVLS+ GKN ME+ +KFRK+EEETIVV  KSLVDID QKDE+LIRARP
Sbjct: 181 GGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARP 240

Query: 279 RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKNYDSHELHAESLYFVHPVTGIPLKLQ 338
           RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGK YDSHELHAESLYFVHP+TGIPLKLQ
Sbjct: 241 RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQ 300

Query: 339 APLPSWASQALQPQQQEVNSPPSFKPMP 367
           APLPSWASQALQPQQQEVNSP SFK MP
Sbjct: 301 APLPSWASQALQPQQQEVNSPQSFKTMP 328

[Close]

BLAST of Lsi11G006250 vs. NCBI nr
Match: KAG7021056.1 (RNA pseudouridine synthase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 601.7 bits (1550), Expect = 4.3e-168
Identity = 312/375 (83.20%), Postives = 330/375 (88.00%), Query Frame = 0


Query: 1   MALFIALQPTFSIPYKTFTKFPFPKPFCFHRFKSVSNSMADSSEPVTGNCPSTRENYPVP 60
           MAL + +QP FSIP+K+F K  FPKPF FHRF+ V+NSMADSS+PVTG      ENYPVP
Sbjct: 6   MALVVTIQPPFSIPHKSFAK--FPKPFRFHRFRPVTNSMADSSKPVTG------ENYPVP 65

Query: 61  LSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCESVLAAVP 120
           LSPPLPAISKNLELARAMAA SKSSLF+LSA DVIYEDEWLIAVNKPQG+YCESVLA+VP
Sbjct: 66  LSPPLPAISKNLELARAMAACSKSSLFSLSACDVIYEDEWLIAVNKPQGVYCESVLASVP 125

Query: 121 RLLGDSAMAG-----IKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSK 180
           R LGDSAMAG     IK SLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDH VSK
Sbjct: 126 RFLGDSAMAGAQSGEIKISLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHKVSK 185

Query: 181 SYIAFCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDMETYFEVLSI 240
           SYIAFCVGSAPKWKKI VKSGHGRSKFGVWRVYAAADVGRSLPGGS+VRDMET FEVLS+
Sbjct: 186 SYIAFCVGSAPKWKKIIVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETSFEVLSV 245

Query: 241 KGKNAMEDCKKFRKD----EEETIVVQIKSLVDIDGQKDEILIRARPRSGRTHQIRLHCQ 300
            G+N  E+  + RKD    EEETIV Q KSL+DID +KDEILIRARPRSGRTHQIRLH Q
Sbjct: 246 NGQNTKEELPESRKDEEEEEEETIVAQSKSLIDIDARKDEILIRARPRSGRTHQIRLHSQ 305

Query: 301 YLGIPIRGDVKYEGVTEWNGKNYDSHELHAESLYFVHPVTGIPLKLQAPLPSWASQALQP 360
           YLGIPI GDVKYEGVTEWNGK YDSHELHAESLYFVHPVTGIPLKLQAPLPSWA+QALQP
Sbjct: 306 YLGIPIVGDVKYEGVTEWNGKIYDSHELHAESLYFVHPVTGIPLKLQAPLPSWATQALQP 365

Query: 361 QQQEVNSPPSFKPMP 367
           QQQEVNS  S KPMP
Sbjct: 366 QQQEVNSAASLKPMP 372

[Close]

BLAST of Lsi11G006250 vs. NCBI nr
Match: KAG6586224.1 (RNA pseudouridine synthase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 593.6 bits (1529), Expect = 1.2e-165
Identity = 310/377 (82.23%), Postives = 327/377 (86.74%), Query Frame = 0


Query: 1   MALFIALQPTFSIPYKTFTKFPFPKPFCFHRFKSVSNSMADSSEPVTGNCPSTRENYPVP 60
           MAL + LQ  FSIP K+F K  FPKPF FHRF+ V+NSMADSS+PVTG      ENYPVP
Sbjct: 1   MALVVTLQAPFSIPNKSFAK--FPKPFRFHRFRPVTNSMADSSKPVTG------ENYPVP 60

Query: 61  LSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCESVLAAVP 120
           LSPPLPAISKNLELARAMAA SKSSLF+LSA DV+YEDEWLIAVNKPQG+YCESVLA+VP
Sbjct: 61  LSPPLPAISKNLELARAMAACSKSSLFSLSACDVVYEDEWLIAVNKPQGVYCESVLASVP 120

Query: 121 RLLGDSAMAG-----IKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSK 180
             LGDSAMAG     IK SLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDH VSK
Sbjct: 121 CFLGDSAMAGEQSGEIKISLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHKVSK 180

Query: 181 SYIAFCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDMETYFEVLSI 240
           SYIAFCVGSAPKWKKI VKSGHGRSKFGVWRVYAAADVGRSLPGGS+VRDMET FEVLS+
Sbjct: 181 SYIAFCVGSAPKWKKIIVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETSFEVLSV 240

Query: 241 KGKNAMEDCKKFRKD------EEETIVVQIKSLVDIDGQKDEILIRARPRSGRTHQIRLH 300
            G+N  E+  + RKD      EEETIV Q KSL+DID +KDEILIRARPRSGRTHQIRLH
Sbjct: 241 NGQNTKEELPESRKDEEEEEEEEETIVAQSKSLIDIDARKDEILIRARPRSGRTHQIRLH 300

Query: 301 CQYLGIPIRGDVKYEGVTEWNGKNYDSHELHAESLYFVHPVTGIPLKLQAPLPSWASQAL 360
            QYLGIPI GDVKYEGVTEWNGK YDSHELHAESLYFVHPVTGIPLKLQAPLPSWA+QAL
Sbjct: 301 SQYLGIPIVGDVKYEGVTEWNGKIYDSHELHAESLYFVHPVTGIPLKLQAPLPSWATQAL 360

Query: 361 QPQQQEVNSPPSFKPMP 367
           QPQQQEVNS  S KPMP
Sbjct: 361 QPQQQEVNSAASLKPMP 369

[Close]

BLAST of Lsi11G006250 vs. TAIR 10
Match: AT1G56345.1 (Pseudouridine synthase family protein )

HSP 1 Score: 360.5 bits (924), Expect = 1.6e-99
Identity = 180/294 (61.22%), Postives = 224/294 (76.19%), Query Frame = 0


Query: 56  NYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCESV 115
           NYP P+S P P ISK++EL RAM ASSKSSLF L+ +D++YEDE+L+AVNKP+G+YCE+V
Sbjct: 39  NYPKPISAPPPPISKDIELRRAMEASSKSSLFNLTRDDILYEDEYLMAVNKPKGVYCEAV 98

Query: 116 LAAVPRLLGDSAMAGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSK 175
           L + P+++ DS+         E HLANRLDRDTSGVM+ITKSHKVA+KLVKAFT+H + K
Sbjct: 99  LRSAPQIVLDSS--------SEYHLANRLDRDTSGVMIITKSHKVAAKLVKAFTEHKIRK 158

Query: 176 SYIAFCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDMETYFEVLSI 235
           SYIA C+GS+P W+++ V SGHGRSK G WRVYAA DVGR LPGGS VRDMET FEV+S+
Sbjct: 159 SYIALCIGSSPNWRRVTVSSGHGRSKHGAWRVYAALDVGRVLPGGSFVRDMETTFEVVSV 218

Query: 236 KG-KNAMEDCKKFRKDEEETIVVQIKSLVDIDGQKDE--ILIRARPRSGRTHQIRLHCQY 295
              KN  E C+   +D    IV + +  +   G  D+  +++RA PRSGRTHQIRLHCQY
Sbjct: 219 NSVKN--ESCE--LEDVNHVIVAEGERELSCGGDDDDVVVVVRAFPRSGRTHQIRLHCQY 278

Query: 296 LGIPIRGDVKYEGVTEWNGKNYDSHELHAESLYFVHPVTGIPLKLQAPLPSWAS 347
           LGIPIRGDVKY GV EWNG+ ++ HELHAE L   HPVTG  + ++APLP WA+
Sbjct: 279 LGIPIRGDVKYHGVYEWNGRTFEGHELHAECLSLDHPVTGDSIVIRAPLPYWAA 320

[Close]

BLAST of Lsi11G006250 vs. TAIR 10
Match: AT1G76050.2 (Pseudouridine synthase family protein )

HSP 1 Score: 70.5 bits (171), Expect = 3.2e-12
Identity = 57/224 (25.45%), Postives = 92/224 (41.07%), Query Frame = 0


Query: 140 LANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVG-SAPKWKKINVKSGHG 199
           + +RLD+ T+G++V+ K     + L + F  HT+ + Y++   G  +P   +I +  G  
Sbjct: 229 IVHRLDKGTTGLLVVAKDEHSHAHLAEQFKLHTIERVYVSLTTGVPSPPQGRIEIPIGRD 288

Query: 200 RSKFGVWRVYAAADVGRSLPGGSIVRDMETYFEVLSIKGKNAMEDCKKFRKDEEETIVVQ 259
            S     R+  AA     +PGG              ++G  A     +++          
Sbjct: 289 SSN----RIRMAA-----IPGG--------------VRGGRARHAASRYK---------- 348

Query: 260 IKSLVDIDGQKDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEG-------VTEWNG 319
              +++        L+  R  +GRTHQIR H +Y+G+P+ GD  Y G       + +   
Sbjct: 349 ---VIETFAGGGSALVEWRLETGRTHQIRAHAKYMGVPLLGDEVYGGTKSMALSLLQKRV 408

Query: 320 KNYDSHE------------LHAESLYFVHPVTGIPLKLQAPLPS 344
              D  E            LHA  L F HP TG  +K   P PS
Sbjct: 409 SRSDQEEIIELISRMDRPCLHAIVLGFTHPCTGEIVKFSCPPPS 416

[Close]

BLAST of Lsi11G006250 vs. TAIR 10
Match: AT3G52260.2 (Pseudouridine synthase family protein )

HSP 1 Score: 60.8 bits (146), Expect = 2.6e-09
Identity = 56/220 (25.45%), Postives = 98/220 (44.55%), Query Frame = 0


Query: 93  DVIYEDEWLIAVNKPQGIYC--------ESVLAAVPRLLG-DSAMAGIKTSLPELHLANR 152
           +V+YED+ LIA+NKP G+           +VL  +    G + +  G + S P     +R
Sbjct: 98  EVLYEDDDLIALNKPSGLQVLPGGLFQQRTVLTQLQWCFGKNDSYIGSRES-PHPVPVHR 157

Query: 153 LDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGSAPKWKKINVKSGHGRSKFG 212
           L R TSG+++  K+    +KL   F + T         VGS      ++ + G GR    
Sbjct: 158 LGRGTSGILLCAKTKLAKTKLAAYFAEGT-------SLVGSG----NLDQECGTGRK--- 217

Query: 213 VWRVYAAADVGRSLPGGSIVRDMETYFEVLSIKGKNAMEDCKKFRKDEEETIVVQIKSLV 272
                  + + R+L  G +  D     + + +     +         E +    ++  ++
Sbjct: 218 ------LSKIYRALADGIVEEDEVVIKQPIGVVRYPGVAQGLYVASPEGKPAFSKV-FVL 277

Query: 273 DIDGQKDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKY 304
           + D +K+  L++   +SGR HQIR+H  Y+G P+ GD  Y
Sbjct: 278 ERDREKNCSLVKVEIQSGRPHQIRIHLAYMGHPLVGDPLY 295

[Close]

BLAST of Lsi11G006250 vs. TAIR 10
Match: AT3G19440.1 (Pseudouridine synthase family protein )

HSP 1 Score: 59.7 bits (143), Expect = 5.7e-09
Identity = 66/231 (28.57%), Postives = 94/231 (40.69%), Query Frame = 0


Query: 94  VIYEDEWLIAVNKPQGIYCESVLAAVPRLLGDSAMAGIKTSLPEL-------------HL 153
           V+Y+D  +I +NKP G             L     +GIKTS+ EL              L
Sbjct: 185 VLYKDPAIIVLNKPHG-------------LAVQGGSGIKTSIDELAASCLKFDKSESPRL 244

Query: 154 ANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGSAPKWKKINVKSGHGRS 213
            +RLDRD SG++V+ ++   A+ L   F + T          G++    K NVKS     
Sbjct: 245 VHRLDRDCSGLLVLARTQTAATVLHSIFREKT---------TGASAYGVKKNVKS----- 304

Query: 214 KFGVWRVYAAADVG-RSLPGGSIVRDMETYFEVLSIKGKNAMEDCKKFR---KDEEETIV 273
              + R Y A  +G      G I   +           K  ++D K  R    D  E + 
Sbjct: 305 ---LKRKYMALVIGCPPRQRGQISAPLR----------KVVVDDGKSERITVNDNGELVS 364

Query: 274 VQ---IKSLVDIDGQKDEILIRARPRSGRTHQIRLHC-QYLGIPIRGDVKY 304
            Q    +  V          +  RP +GR HQ+R+HC + LG PI GD KY
Sbjct: 365 TQHAITEYRVIESSPHGYTWLELRPLTGRKHQLRVHCAEVLGTPIVGDYKY 375

[Close]

BLAST of Lsi11G006250 vs. TAIR 10
Match: AT3G52260.1 (Pseudouridine synthase family protein )

HSP 1 Score: 55.8 bits (133), Expect = 8.2e-08
Identity = 53/214 (24.77%), Postives = 95/214 (44.39%), Query Frame = 0


Query: 93  DVIYEDEWLIAVNKPQGIYC--------ESVLAAVPRLLG-DSAMAGIKTSLPELHLANR 152
           +V+YED+ LIA+NKP G+           +VL  +    G + +  G + S P     +R
Sbjct: 98  EVLYEDDDLIALNKPSGLQVLPGGLFQQRTVLTQLQWCFGKNDSYIGSRES-PHPVPVHR 157

Query: 153 LDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGSAPKWKKINVKSGHGRSKFG 212
           L R TSG+++  K+    +KL   F + T         VGS      ++ + G GR    
Sbjct: 158 LGRGTSGILLCAKTKLAKTKLAAYFAEGT-------SLVGSG----NLDQECGTGRK--- 217

Query: 213 VWRVYAAADVGRSLPGGSIVRDMETYFEVLSIKGKNAMEDCKKFRKDEEETIVVQIKSLV 272
                  + + R+L  G +  D     + + +     +         E +    ++  ++
Sbjct: 218 ------LSKIYRALADGIVEEDEVVIKQPIGVVRYPGVAQGLYVASPEGKPAFSKV-FVL 277

Query: 273 DIDGQKDEILIRARPRSGRTHQIRLHCQYLGIPI 298
           + D +K+  L++   +SGR HQIR+H  Y+G P+
Sbjct: 278 ERDREKNCSLVKVEIQSGRPHQIRIHLAYMGHPL 289

The following BLAST results are available for this feature:

BLAST of Lsi11G006250 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q2QNM3	2.3e-100	61.36	RNA pseudouridine synthase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g05...	[more]
Q7XA65	2.2e-98	61.22	RNA pseudouridine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g56345 PE=2 S...	[more]
Q68XB2	2.2e-18	31.64	Ribosomal large subunit pseudouridine synthase C OS=Rickettsia typhi (strain ATC...	[more]
Q9ZDR7	6.5e-18	30.47	Ribosomal large subunit pseudouridine synthase C OS=Rickettsia prowazekii (strai...	[more]
Q87S65	7.2e-17	26.37	Ribosomal large subunit pseudouridine synthase D OS=Vibrio parahaemolyticus sero...	[more]

BLAST of Lsi11G006250 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LJY6	9.0e-188	90.71	PseudoU_synth_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G03...	[more]
A0A5A7TX25	4.1e-172	92.68	RNA pseudouridine synthase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A1S3BW87	4.1e-172	92.68	RNA pseudouridine synthase 1 OS=Cucumis melo OX=3656 GN=LOC103494301 PE=4 SV=1	[more]
A0A6J1DQ12	8.8e-159	86.36	RNA pseudouridine synthase 1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A6J1DT56	1.3e-157	83.82	RNA pseudouridine synthase 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111...	[more]

BLAST of Lsi11G006250 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004144814.3	1.9e-187	90.71	RNA pseudouridine synthase 1 [Cucumis sativus] >KGN60992.1 hypothetical protein ...	[more]
XP_038889468.1	7.8e-186	92.48	RNA pseudouridine synthase 1 [Benincasa hispida]	[more]
XP_008453651.1	8.4e-172	92.68	PREDICTED: RNA pseudouridine synthase 1 [Cucumis melo] >KAA0048062.1 RNA pseudou...	[more]
KAG7021056.1	4.3e-168	83.20	RNA pseudouridine synthase 1, partial [Cucurbita argyrosperma subsp. argyrosperm...	[more]
KAG6586224.1	1.2e-165	82.23	RNA pseudouridine synthase 1, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Lsi11G006250 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G56345.1	1.6e-99	61.22	Pseudouridine synthase family protein	[more]
AT1G76050.2	3.2e-12	25.45	Pseudouridine synthase family protein	[more]
AT3G52260.2	2.6e-09	25.45	Pseudouridine synthase family protein	[more]
AT3G19440.1	5.7e-09	28.57	Pseudouridine synthase family protein	[more]
AT3G52260.1	8.2e-08	24.77	Pseudouridine synthase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006145	Pseudouridine synthase, RsuA/RluA-like	PFAM	PF00849	PseudoU_synth_2	coord: 101..291 e-value: 1.1E-22 score: 80.8
IPR006145	Pseudouridine synthase, RsuA/RluA-like	CDD	cd02869	PseudoU_synth_RluA_like	coord: 101..326 e-value: 2.47755E-39 score: 136.313
None	No IPR available	GENE3D	3.30.2350.10	Pseudouridine synthase	coord: 89..342 e-value: 1.0E-45 score: 157.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 346..366
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 346..360
None	No IPR available	PANTHER	PTHR21600:SF47	RNA PSEUDOURIDINE SYNTHASE 1	coord: 51..353
None	No IPR available	PANTHER	PTHR21600	MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE	coord: 51..353
IPR006224	Pseudouridine synthase, RluC/RluD, conserved site	PROSITE	PS01129	PSI_RLU	coord: 141..155
IPR020103	Pseudouridine synthase, catalytic domain superfamily	SUPERFAMILY	55120	Pseudouridine synthase	coord: 92..351

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi11G006250.1	Lsi11G006250.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0001522	pseudouridine synthesis
biological_process	GO:0009451	RNA modification
molecular_function	GO:0009982	pseudouridine synthase activity
molecular_function	GO:0003723	RNA binding