Lsi11G004940 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi11G004940
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionTerpene cyclase/mutase family member
Locationchr11: 5208109 .. 5212392 (-)
RNA-Seq ExpressionLsi11G004940
SyntenyLsi11G004940
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTTCTTTGCAAATCCTTGAATTTCGATCCATCATGTGGAGACTTAAGGTAGGCGATGGTGGAAATGATCCTTACATCTATTCCGTGAACAACTTCGTAGGAAGGCAAATATGGGAATTTGACCCAAACGCTGGAACTCCTGAAGAGCGAGCCGAAGTCGAATGCCTTCGCCACAATTTCACCAAAAATTGTCTCAAGAGTTTTTCAAGCGCTGACTTGCTTTGGCGCCTTCAGGTAACACTATTTCCAATTTTTCAAGAATAGTTACATTTAATTTTTATGATTTTAACTTGACCATTTTAGTCCTTCAATTTTAAAAAATAGATTTAAAAGTTATTTATTTCAATTTTGTTACATTAATTCGATATTTTCTTAAATTATTTTAAATATAACATGACATATTTAAATTAAATAATAAAAAAAATACTCTCTCTTCTCTCTTAATATCCTCCTCTCTCCCTCCACCACATCTTCAAAATTTATATCAGTCAAAAAAAATAAGGTGGTATTAGTTAAACTTTTTTTTTCTTTATGCTAGAACTCTTATAGAGGTCGATCATCAATTTAAAGAGAAGAAATATATATCAATCATCGTTAAGCTATACTCACATTCATATTTTTTTTTAGTTTTTGAATAGAGAGATAATAATTTGGATGCAGCTTCTCAGAGAGAAAAATTTCAAACAAAATATCCCACGAGTGAAGGTTAAAGATGGAGAAGAAATAAATTACGAAATGGCATCAAATGCAACGAGGAGAGGGGCGTATTTCTTAGCGGCCATACAAACAAGTGATGGACATTGGCCTAGTGAAACATCGGGCCCTTTATTTTACATTTGTCCAATGCTAATTTGCATGTACATTATGGGTTTCATGGATAAAGTATTCAGTCCAGAGCACAAGAAAGAAATGTTGCGTTACATTTACAACCATCAGGTAAAACTATTCCTCTATGAAATTTTACTACATAGAAAAATTATTACAATCATACAAAATTTTTTTCCCGACTTTAGATACAACGGTTTGAATTAACTTTTTAAATAAAAAATATATACTTCACCATAGATTGCTCTCTCCTCTAAAATTTTGTCAACATCCTGCAAATTCCACATTGCAAGATATATTTGAAAAATTCATTGCTCTTACTGCATTTATTATCTTTATTGACAATTCAGAAAAATTATAACATAGAGAAAATTTGACTTGAAAAATTATTAAAATAACACTAAAATTTCGTATAGTTTTATATACTATTGAACACAAATAGTGTAGTATATAGGGGTGAGCATGGATAGCCGAAAAATTGAGCCGAACGATTGAAACTGGCCGAACCGAAGGTTGGAGGTCAGTTTTTAAGAAAAAACGGTTGGTGTCCGTTTTGTCAAAATACAATTAAAAACTGACCAAAACCGACCGAGTATTAAATCGAATCGGACCACCACCACTTGATTATTATATAAATAAATAAAAATATATATTAATATTATATATTATTAGATAACTAAAAATATTAAATCATATATATTTTAGGTAATGATTACCTACTTATAAAAAATATTTCTTTTATAATATTTTAGTTTTTCAAAAAAAAAAAAAAAATTACTCTTTCAATTCTTCCTCCTCTCTCTCTCTTGCCTTTCTTATTTTTCTTTTTCTTTCTTTTATTTTATATTCTCTTCTCCATTCTTCTTCATTTCTCTCCCTCATTTTGTCATTCTTCTCTCTGTTTATCTCCCTCCTTTCTTCATAGTTCATTCCGTTTTCTTTTTTCCTAGTTTTTTTTTTTCTTTTTTTTTTCTTATTTATTTATTTTTTTACTGTTACTTTAGGTTTTTTCCGTTCAGCTCGCCAGATATTCACCCCAGTAGCTGGACCAAACCAAGTTGTTGGTTTTTTTGTTCCATTATTTTTAATTAAAGACATCCATGTTTTTTAAAAACTATGAATAGAAAAATTTTGCAATTGACCATGGATGCAAGCGCGCATCTTATGATTCAAATGCAAAAACGGATTTTTAAAAATGATATTTTTTTTCCAAAATTCCTTAACAAATTTAAAAACGTTTTTAAATTTAAAAAAAAAAAAAAAAAAAAAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAAAAAAAAAAAAAAAAAAACAAAAGAATCAATTCGTTTCCAAACATTCATTAAAACAAAAAAAGCATTAGTTATCAAATAGCTCCTTAAATATAGGTAGAGACTAGTGAACATGAGTCATAGCTTAAATTAATTAAGATGTACTCTATGTTTTGAGATCGAAATTCTCATTTCTCATATCTAATGGAATAATAATAATATTTTACAGAATGAAGATGGGGAATGGGCATTACACATAGGTAGTCACAGTAGCATGTTCTGCACCGCCCTCAATTATATCTCTTTACGTTTACTTGGTGAGGGACCTGATCTTGAGCCACTTGCCATAGCCAGAAATTGGATCCGACATCGCGGCGGCGTCACCTCCATACCTTCCTGGGGAAAGACCTGGCTCTCGGTATTACTTCGTGTGTTTTGAAATTTATTCAGTTTAAGCTCCGTTTGCAATTGTAATAAGTGATTTTGGGAAAATTTTAACCGCCTTCATTATTTGGTAAATCAAATTATTAATTAATCCAAGATAATCACTTTTTAAATTTTAACTAACGAGTGAAAACGTATGGTAGTAGCTTTTAATTAGATTCAACTTTTCTTAAAAAAACTACTAATTTCTTAACAAATAAGGAATGTTTATAAAAATAAAAAAAATATTAAACTATTTATGAATATAGAAAAATTTCATTGTCTATCTACGATAAACCGCGATAGACTTCTATAGCTCAGTGATAGAAGTTTATCACAGATAGATGATGAAATTTTTATATATTTATAAATAGTTGGATATTTTTTCCATTTATAATAATTTTCCTTTTAATAAATAAATTATAATAATATTTTTCTATATTAATAGATTTTTCACATTTATTTATAAACTATAAACTTTAATTGATTCAATTTTTTCATTTATTTATAAACTATAAACTTTAATTGATTCAATTTTTTTATAACAATTACTTTTTAAAAATTTCATCAAATAATTTAGATTAAACAAAATTAGTTGAAATTCAAATCATATAAACTTTTAAAAAATATATATTATAATAACTTATAAAATAATTATCAATATGAATATGATTAAAATAATAAGCCTACTTTGATTCATTTAATTAAATTATACCAATTTAGTATTAATTTTATCCTACATCGTAATTAATTTTTCTAATTTAAAGCTAAAATTTAATACTTCCTTTCCTAGTTCATTTTTCACAAAGGAAAGTTGACAACAATTATTTTAACCGCCAATCTCAAACAAGGTTTTTTTTTTTTTTTTTTTTTGTATTTTTCAAATGTCCAATTTTAATTAGGGTATACCTACTTTCAATAAATTTTAAATTTTGTTATTCAAAATTAATTTTCATGCTAGAAAATGCATGGTTTGTATTCCCTTTTTCAATATATACAAGAAATGCATGTAGATAATAAATTTTTTTTCTTGAAAAAATCTACGGAGATTAAATTTAGATATTTAAAATTACAGAAACTAAATTGAAAAAAATCTCAAAGTACAATCTATTTTTTAATGTCCTTTCTTACCCATCCAACCTTTATTATATTATTATTAGAAAAATATATATATTAAGTACATTCTAGACTATATCTATATTTGTGCTATTTTTCACATTTTTTCTTACTTCTTTTAAAAAAAAAAATTTAATTTTTTTTTTTTGAAGTGTGAAACTTACATAATTTTAGTTGGGTAATGTAAAGATATTAAACGTGTTCGAGTGGTGTGGAAGCAACCCCATGCCTCCTGAATGTTGGATGCTTCCCAGTTGGATCCCCATTCACCCATGTAACTCCAACTTATAA

mRNA sequence

GTTTCTTTGCAAATCCTTGAATTTCGATCCATCATGTGGAGACTTAAGGTAGGCGATGGTGGAAATGATCCTTACATCTATTCCGTGAACAACTTCGTAGGAAGGCAAATATGGGAATTTGACCCAAACGCTGGAACTCCTGAAGAGCGAGCCGAATTTTTGAATAGAGAGATAATAATTTGGATGCAGCTTCTCAGAGAGAAAAATTTCAAACAAAATATCCCACGAGTGAAGGTTAAAGATGGAGAAGAAATAAATTACGAAATGGCATCAAATGCAACGAGGAGAGGGGCGTATTTCTTAGCGGCCATACAAACAAGTGATGGACATTGGCCTAGTGAAACATCGGGCCCTTTATTTTACATTTGTCCAATGCTAATTTGCATGTACATTATGGGTTTCATGGATAAAGTATTCAGTCCAGAGCACAAGAAAGAAATGTTGCGTTACATTTACAACCATCAGAATGAAGATGGGGAATGGGCATTACACATAGGTAGTCACAGTAGCATGTTCTGCACCGCCCTCAATTATATCTCTTTACGTTTACTTGGTGAGGGACCTGATCTTGAGCCACTTGCCATAGCCAGAAATTGGATCCGACATCGCGGCGGCATATTAAACGTGTTCGAGTGGTGTGGAAGCAACCCCATGCCTCCTGAATGTTGGATGCTTCCCAGTTGGATCCCCATTCACCCATGTAACTCCAACTTATAA

Coding sequence (CDS)

GTTTCTTTGCAAATCCTTGAATTTCGATCCATCATGTGGAGACTTAAGGTAGGCGATGGTGGAAATGATCCTTACATCTATTCCGTGAACAACTTCGTAGGAAGGCAAATATGGGAATTTGACCCAAACGCTGGAACTCCTGAAGAGCGAGCCGAATTTTTGAATAGAGAGATAATAATTTGGATGCAGCTTCTCAGAGAGAAAAATTTCAAACAAAATATCCCACGAGTGAAGGTTAAAGATGGAGAAGAAATAAATTACGAAATGGCATCAAATGCAACGAGGAGAGGGGCGTATTTCTTAGCGGCCATACAAACAAGTGATGGACATTGGCCTAGTGAAACATCGGGCCCTTTATTTTACATTTGTCCAATGCTAATTTGCATGTACATTATGGGTTTCATGGATAAAGTATTCAGTCCAGAGCACAAGAAAGAAATGTTGCGTTACATTTACAACCATCAGAATGAAGATGGGGAATGGGCATTACACATAGGTAGTCACAGTAGCATGTTCTGCACCGCCCTCAATTATATCTCTTTACGTTTACTTGGTGAGGGACCTGATCTTGAGCCACTTGCCATAGCCAGAAATTGGATCCGACATCGCGGCGGCATATTAAACGTGTTCGAGTGGTGTGGAAGCAACCCCATGCCTCCTGAATGTTGGATGCTTCCCAGTTGGATCCCCATTCACCCATGTAACTCCAACTTATAA

Protein sequence

VSLQILEFRSIMWRLKVGDGGNDPYIYSVNNFVGRQIWEFDPNAGTPEERAEFLNREIIIWMQLLREKNFKQNIPRVKVKDGEEINYEMASNATRRGAYFLAAIQTSDGHWPSETSGPLFYICPMLICMYIMGFMDKVFSPEHKKEMLRYIYNHQNEDGEWALHIGSHSSMFCTALNYISLRLLGEGPDLEPLAIARNWIRHRGGILNVFEWCGSNPMPPECWMLPSWIPIHPCNSNL
Homology
BLAST of Lsi11G004940 vs. ExPASy Swiss-Prot
Match: Q948R6 (Isomultiflorenol synthase OS=Luffa aegyptiaca OX=3670 GN=IMS1 PE=1 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 7.8e-105
Identity = 176/252 (69.84%), Postives = 197/252 (78.17%), Query Frame = 0

Query: 12  MWRLKVGDGGNDPYIYSVNNFVGRQIWEFDPNAGTPEERAE---------------FLNR 71
           MWRLKV DGGNDPYIYS+NNF+GRQIWEFDPNAGTPEERAE               F + 
Sbjct: 1   MWRLKVADGGNDPYIYSMNNFIGRQIWEFDPNAGTPEERAEIERLRHHFTKNRHKGFPSA 60

Query: 72  EIIIWMQLLREKNFKQNIPRVKVKDGEEINYEMASNATRRGAYFLAAIQTSDGHWPSETS 131
           +++  +QLLREKNFKQ+IP VKV DGEEI+YEMA +A RRGA+FLAAIQ SDGHWPSETS
Sbjct: 61  DLLWRVQLLREKNFKQSIPAVKVGDGEEISYEMALDAMRRGAHFLAAIQASDGHWPSETS 120

Query: 132 GPLFYICPMLICMYIMGFMDKVFSPEHKKEMLRYIYNHQNEDGEWALHIGSHSSMFCTAL 191
           GPLFY+CP+LICMYIMGFMDKVFSPEHKKEM+RYIYNHQNEDG W LH+G HS+MFCT  
Sbjct: 121 GPLFYVCPLLICMYIMGFMDKVFSPEHKKEMMRYIYNHQNEDGGWGLHVGGHSNMFCTTF 180

Query: 192 NYISLRLLGEGPDLEPLAIARNWIRHRGG-------------ILNVFEWCGSNPMPPECW 236
           NYISLRLLGE PD+E +  ARNWI    G             ILNVF+W  SNPMPPE W
Sbjct: 181 NYISLRLLGEEPDVEAVCKARNWIHDHDGVTSILSWGKTWLSILNVFDWSASNPMPPEYW 240

BLAST of Lsi11G004940 vs. ExPASy Swiss-Prot
Match: Q9MB42 (Beta-amyrin synthase OS=Glycyrrhiza glabra OX=49827 GN=GgbAS1 PE=1 SV=1)

HSP 1 Score: 308.9 bits (790), Expect = 4.9e-83
Identity = 144/253 (56.92%), Postives = 176/253 (69.57%), Query Frame = 0

Query: 12  MWRLKVGDGGNDPYIYSVNNFVGRQIWEFDPNAGTPEERAE-------FLNREI------ 71
           MWRLK+ +GG DPYIYS NNFVGRQ WE+DP+ GTPEERA+       F N         
Sbjct: 1   MWRLKIAEGGKDPYIYSTNNFVGRQTWEYDPDGGTPEERAQVDAARLHFYNNRFQVKPCG 60

Query: 72  -IIW-MQLLREKNFKQNIPRVKVKDGEEINYEMASNATRRGAYFLAAIQTSDGHWPSETS 131
            ++W  Q+LRE NFKQ I  VK+ DGEEI YE A+ A RR A+ L+A+QTSDGHWP++ +
Sbjct: 61  DLLWRFQILRENNFKQTIASVKIGDGEEITYEKATTAVRRAAHHLSALQTSDGHWPAQIA 120

Query: 132 GPLFYICPMLICMYIMGFMDKVFSPEHKKEMLRYIYNHQNEDGEWALHIGSHSSMFCTAL 191
           GPLF++ P++ CMYI G +D VF  E++KE+LRYIY HQNEDG W LHI  HS+MFCTAL
Sbjct: 121 GPLFFLPPLVFCMYITGHLDSVFPEEYRKEILRYIYYHQNEDGGWGLHIEGHSTMFCTAL 180

Query: 192 NYISLRLLGEGPD---LEPLAIARNWIRHRGG-------------ILNVFEWCGSNPMPP 234
           NYI +R+LGEGPD       A AR WI   GG             IL VF+WCGSNPMPP
Sbjct: 181 NYICMRILGEGPDGGQDNACARARKWIHDHGGVTHIPSWGKTWLSILGVFDWCGSNPMPP 240

BLAST of Lsi11G004940 vs. ExPASy Swiss-Prot
Match: Q8W3Z1 (Beta-amyrin synthase OS=Betula platyphylla OX=78630 GN=OSCBPY PE=1 SV=1)

HSP 1 Score: 306.6 bits (784), Expect = 2.4e-82
Identity = 144/253 (56.92%), Postives = 179/253 (70.75%), Query Frame = 0

Query: 12  MWRLKVGDGGNDPYIYSVNNFVGRQIWEFDPNAGTPEERAE--------FLNR------- 71
           MWRLK+ DGG+DPYIYS NNFVGRQ WEFDP AG+P+ERAE        + NR       
Sbjct: 1   MWRLKIADGGSDPYIYSTNNFVGRQTWEFDPQAGSPQERAEVEEARRNFYDNRYQVKPSG 60

Query: 72  EIIIWMQLLREKNFKQNIPRVKVKDGEEINYEMASNATRRGAYFLAAIQTSDGHWPSETS 131
           +++  MQ L+EKNFKQ IP VKV+DGEEI YE ++ A RR  +F +A+Q SDGHWP+E +
Sbjct: 61  DLLWRMQFLKEKNFKQTIPPVKVEDGEEITYEKSTAALRRAVHFYSALQASDGHWPAENA 120

Query: 132 GPLFYICPMLICMYIMGFMDKVFSPEHKKEMLRYIYNHQNEDGEWALHIGSHSSMFCTAL 191
           GPLF++ P+++CMYI G ++ VF  EH+KE+LRYIY HQNEDG W LHI  HS+MFCTAL
Sbjct: 121 GPLFFLPPLVMCMYITGHLNTVFPAEHQKEILRYIYYHQNEDGGWGLHIEGHSTMFCTAL 180

Query: 192 NYISLRLLGEGPD---LEPLAIARNWIRHRGG-------------ILNVFEWCGSNPMPP 234
           +YI +R+LGEGPD       A AR WI   GG             IL +FEW GSNPMPP
Sbjct: 181 SYICMRILGEGPDGGQDNACARARKWILDHGGVTHMPSWGKTWLSILGIFEWIGSNPMPP 240

BLAST of Lsi11G004940 vs. ExPASy Swiss-Prot
Match: Q9LRH8 (Beta-amyrin synthase OS=Pisum sativum OX=3888 GN=OSCPSY PE=2 SV=1)

HSP 1 Score: 301.6 bits (771), Expect = 7.9e-81
Identity = 140/253 (55.34%), Postives = 174/253 (68.77%), Query Frame = 0

Query: 12  MWRLKVGDGGNDPYIYSVNNFVGRQIWEFDPNAGTPEERAE-------FLNREI------ 71
           MWRLK+ +GGNDPY++S NNFVGRQ WE+DP AG+ EERA+       F N         
Sbjct: 1   MWRLKIAEGGNDPYLFSTNNFVGRQTWEYDPEAGSEEERAQVEEARRNFYNNRFEVKPCG 60

Query: 72  -IIW-MQLLREKNFKQNIPRVKVKDGEEINYEMASNATRRGAYFLAAIQTSDGHWPSETS 131
            ++W  Q+LRE NFKQ I  VK++D EEI YE  +   RRG + LA +QTSDGHWP++ +
Sbjct: 61  DLLWRFQVLRENNFKQTIGGVKIEDEEEITYEKTTTTLRRGTHHLATLQTSDGHWPAQIA 120

Query: 132 GPLFYICPMLICMYIMGFMDKVFSPEHKKEMLRYIYNHQNEDGEWALHIGSHSSMFCTAL 191
           GPLF++ P++ C+YI G +D VF PEH+KE+LRYIY HQNEDG W LHI  HS+MFCTAL
Sbjct: 121 GPLFFMPPLVFCVYITGHLDSVFPPEHRKEILRYIYCHQNEDGGWGLHIEGHSTMFCTAL 180

Query: 192 NYISLRLLGEGPD---LEPLAIARNWIRHRGG-------------ILNVFEWCGSNPMPP 234
           NYI +R+LGEGPD         ARNWIR  GG             IL VF+W GSNPMPP
Sbjct: 181 NYICMRILGEGPDGGEDNACVRARNWIRQHGGVTHIPSWGKTWLSILGVFDWLGSNPMPP 240

BLAST of Lsi11G004940 vs. ExPASy Swiss-Prot
Match: A8C980 (Germanicol synthase OS=Rhizophora stylosa OX=98588 GN=M1 PE=1 SV=1)

HSP 1 Score: 299.3 bits (765), Expect = 3.9e-80
Identity = 138/253 (54.55%), Postives = 177/253 (69.96%), Query Frame = 0

Query: 12  MWRLKVGDGGNDPYIYSVNNFVGRQIWEFDPNAGTPEERAE--------FLNR------- 71
           MWRLK+ +GGNDPY+YS NN+VGRQIWEFDP+AGTPEERA+        + NR       
Sbjct: 1   MWRLKIAEGGNDPYLYSTNNYVGRQIWEFDPDAGTPEERAKAEEARQNFYKNRYQVKPSG 60

Query: 72  EIIIWMQLLREKNFKQNIPRVKVKDGEEINYEMASNATRRGAYFLAAIQTSDGHWPSETS 131
           +++  +Q LREKNFKQ IP+V++++GEEI  E A+ A RR   F +A+Q SDGHWP+E +
Sbjct: 61  DLLWRLQFLREKNFKQTIPQVRIEEGEEITREKATTALRRAVQFFSALQASDGHWPAENA 120

Query: 132 GPLFYICPMLICMYIMGFMDKVFSPEHKKEMLRYIYNHQNEDGEWALHIGSHSSMFCTAL 191
           GPLF++ P+++CM I G +D VF  EH+KE+LRYIY HQNEDG W LHI  HS+MFCTAL
Sbjct: 121 GPLFFLPPLVMCMCITGHLDTVFPAEHRKEILRYIYYHQNEDGGWGLHIEGHSTMFCTAL 180

Query: 192 NYISLRLLGEGPD---LEPLAIARNWIRHRG-------------GILNVFEWCGSNPMPP 234
           NYI +R+LGEGP+    +    AR WI   G              IL V++W G NPMPP
Sbjct: 181 NYICMRILGEGPNGGQDDACTRARKWIHDHGSVTNIPSWGKTWLSILGVYDWSGCNPMPP 240

BLAST of Lsi11G004940 vs. ExPASy TrEMBL
Match: A0A1S4DZT5 (Terpene cyclase/mutase family member OS=Cucumis melo OX=3656 GN=LOC103495616 PE=3 SV=1)

HSP 1 Score: 406.4 bits (1043), Expect = 8.4e-110
Identity = 193/251 (76.89%), Postives = 205/251 (81.67%), Query Frame = 0

Query: 12  MWRLKVGDGGNDPYIYSVNNFVGRQIWEFDPNAGTPEERAE---------------FLNR 71
           MWRLKVG+ GNDPYIYSVNNFVGRQIWEFDPNAGTPEERAE               F + 
Sbjct: 1   MWRLKVGESGNDPYIYSVNNFVGRQIWEFDPNAGTPEERAEVERVRYNFTKNRRKGFPSA 60

Query: 72  EIIIWMQLLREKNFKQNIPRVKVKDGEEINYEMA-SNATRRGAYFLAAIQTSDGHWPSET 131
           ++   +Q LREK FKQ+IP+VKV+DGEEI+YE A SNA RRGA+FLAAIQ SDGHWPSET
Sbjct: 61  DLFWRLQFLREKKFKQSIPQVKVEDGEEISYEKASSNAMRRGAHFLAAIQASDGHWPSET 120

Query: 132 SGPLFYICPMLICMYIMGFMDKVFSPEHKKEMLRYIYNHQNEDGEWALHIGSHSSMFCTA 191
           SGPLFYICPMLIC+YIMG MDKVFSPEHKKEMLRYIYNHQNEDG WALHIGSHSSMFCT 
Sbjct: 121 SGPLFYICPMLICIYIMGCMDKVFSPEHKKEMLRYIYNHQNEDGGWALHIGSHSSMFCTT 180

Query: 192 LNYISLRLLGEGPDLEPLAIARNWIRHRGG-------------ILNVFEWCGSNPMPPEC 234
           LNYISLRLLGEGPD EPL IARNWIR RGG             ILNVF+WCGSNPMPPEC
Sbjct: 181 LNYISLRLLGEGPDYEPLVIARNWIRQRGGVTYIPSWGKTWLSILNVFDWCGSNPMPPEC 240

BLAST of Lsi11G004940 vs. ExPASy TrEMBL
Match: A0A0A0LFN0 (SQHop_cyclase_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G014580 PE=4 SV=1)

HSP 1 Score: 389.0 bits (998), Expect = 1.4e-104
Identity = 178/254 (70.08%), Postives = 200/254 (78.74%), Query Frame = 0

Query: 12  MWRLKVGDGGNDPYIYSVNNFVGRQIWEFDPNAGTPEERAE-----------------FL 71
           MWRLKVGDGGNDPYIYS+NNFVGRQIWEFDPNAGTPEER E                 FL
Sbjct: 1   MWRLKVGDGGNDPYIYSMNNFVGRQIWEFDPNAGTPEERVEVERLRQNFINKRHKENSFL 60

Query: 72  NREIIIWMQLLREKNFKQNIPRVKVKDGEEINYEMASNATRRGAYFLAAIQTSDGHWPSE 131
           + +++  +Q LREK FKQ+IP+ KV+DGEEI+YE ASNA RRGAYFLAAIQ SDGHWPSE
Sbjct: 61  SADLLWRLQFLREKKFKQSIPQEKVEDGEEISYEKASNAMRRGAYFLAAIQASDGHWPSE 120

Query: 132 TSGPLFYICPMLICMYIMGFMDKVFSPEHKKEMLRYIYNHQNEDGEWALHIGSHSSMFCT 191
           TSGPLFY+CPMLIC+Y+MG MD + SPEHKKEMLRYIYNHQNEDG W LH+G HS+MFCT
Sbjct: 121 TSGPLFYMCPMLICIYVMGIMDTILSPEHKKEMLRYIYNHQNEDGGWGLHVGGHSNMFCT 180

Query: 192 ALNYISLRLLGEGPDLEPLAIARNWIRHRGG-------------ILNVFEWCGSNPMPPE 236
             NYISLRLLGEGP++E L  +RNWIRHRGG             ILNVF+W GSNPMPPE
Sbjct: 181 TFNYISLRLLGEGPEVEQLFRSRNWIRHRGGVTSIPSWGKTWLSILNVFDWSGSNPMPPE 240

BLAST of Lsi11G004940 vs. ExPASy TrEMBL
Match: A0A0A0LG93 (Terpene cyclase/mutase family member OS=Cucumis sativus OX=3659 GN=Csa_2G013830 PE=3 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 5.8e-103
Identity = 174/252 (69.05%), Postives = 201/252 (79.76%), Query Frame = 0

Query: 12  MWRLKVGDGGNDPYIYSVNNFVGRQIWEFDPNAGTPEERAE---------------FLNR 71
           MWRLK+G+GGNDPYIYS+NNFVGRQIWEFDPNAGTPEE+AE               F + 
Sbjct: 1   MWRLKIGEGGNDPYIYSMNNFVGRQIWEFDPNAGTPEEQAEIEHLRQRFTKNHLKGFPSG 60

Query: 72  EIIIWMQLLREKNFKQNIPRVKVKDGEEINYEMASNATRRGAYFLAAIQTSDGHWPSETS 131
           +++  +Q LREK FKQ+IP+VKV+DGEEI+Y+ ASNA RRGAYFLAAIQ SDGHWPSE+S
Sbjct: 61  DLLWRLQFLREKKFKQSIPQVKVEDGEEISYDKASNAMRRGAYFLAAIQASDGHWPSESS 120

Query: 132 GPLFYICPMLICMYIMGFMDKVFSPEHKKEMLRYIYNHQNEDGEWALHIGSHSSMFCTAL 191
           GPLFY+CPMLICMYIMG MD + SPEHKKEMLRY+YNHQNEDG W LH+G HS+MFCT  
Sbjct: 121 GPLFYLCPMLICMYIMGTMDTILSPEHKKEMLRYVYNHQNEDGGWGLHVGGHSNMFCTTF 180

Query: 192 NYISLRLLGEGPDLEPLAIARNWIRHRGG-------------ILNVFEWCGSNPMPPECW 236
           NYISLRLLGEGP++E L+ +RNWIR RGG             ILNVF+W GSNPMPPE W
Sbjct: 181 NYISLRLLGEGPEVEQLSRSRNWIRQRGGVTSIPSWGKTWLSILNVFDWSGSNPMPPEYW 240

BLAST of Lsi11G004940 vs. ExPASy TrEMBL
Match: A0A5A7VDU0 (Isomultiflorenol synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold329G001740 PE=3 SV=1)

HSP 1 Score: 380.6 bits (976), Expect = 4.9e-102
Identity = 174/252 (69.05%), Postives = 197/252 (78.17%), Query Frame = 0

Query: 12  MWRLKVGDGGNDPYIYSVNNFVGRQIWEFDPNAGTPEERAE---------------FLNR 71
           MWRLKVG+ GNDPYIYSVNNFVGRQIWEFDPNAGTPEERAE               F + 
Sbjct: 1   MWRLKVGESGNDPYIYSVNNFVGRQIWEFDPNAGTPEERAEVERVRYNFTKNRRKGFPSA 60

Query: 72  EIIIWMQLLREKNFKQNIPRVKVKDGEEINYEMASNATRRGAYFLAAIQTSDGHWPSETS 131
           ++   +Q LREK FKQ+IP+ KV+DGEEI+YE ASNA RRGA+FLAAIQ SDGHWPSETS
Sbjct: 61  DLFWRLQFLREKKFKQSIPQEKVEDGEEISYEKASNAMRRGAHFLAAIQASDGHWPSETS 120

Query: 132 GPLFYICPMLICMYIMGFMDKVFSPEHKKEMLRYIYNHQNEDGEWALHIGSHSSMFCTAL 191
           GP FY+CPMLIC+YIMG MD + SPEHKKEMLRY+YNHQNEDG W LH+G HS+MFCT  
Sbjct: 121 GPQFYLCPMLICIYIMGIMDTILSPEHKKEMLRYVYNHQNEDGGWGLHVGGHSNMFCTTF 180

Query: 192 NYISLRLLGEGPDLEPLAIARNWIRHRGG-------------ILNVFEWCGSNPMPPECW 236
           NYISLRLLGEGP++E L+ +RNWIRH GG             ILNVF+W GSNPMPPE W
Sbjct: 181 NYISLRLLGEGPEVEELSKSRNWIRHHGGVTSIPSWGKTWLSILNVFDWSGSNPMPPEYW 240

BLAST of Lsi11G004940 vs. ExPASy TrEMBL
Match: A0A5D3BXV9 (H(+)-exporting diphosphatase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G001980 PE=3 SV=1)

HSP 1 Score: 376.7 bits (966), Expect = 7.1e-101
Identity = 171/252 (67.86%), Postives = 195/252 (77.38%), Query Frame = 0

Query: 12  MWRLKVGDGGNDPYIYSVNNFVGRQIWEFDPNAGTPEERAE---------------FLNR 71
           MWRLKV DGGNDPYIYS+NNFVGRQIWEFDPNAGTPEERAE               F + 
Sbjct: 1   MWRLKVADGGNDPYIYSMNNFVGRQIWEFDPNAGTPEERAEVERIQNEFTTNRFKGFPSA 60

Query: 72  EIIIWMQLLREKNFKQNIPRVKVKDGEEINYEMASNATRRGAYFLAAIQTSDGHWPSETS 131
           +++  +QLLREKNFKQ+IP VK+++GEE++YE A +A RRGA+FLAAIQ SDGHWPSETS
Sbjct: 61  DLLWRLQLLREKNFKQSIPAVKIEEGEEVSYEKAWDAMRRGAHFLAAIQASDGHWPSETS 120

Query: 132 GPLFYICPMLICMYIMGFMDKVFSPEHKKEMLRYIYNHQNEDGEWALHIGSHSSMFCTAL 191
           GPLFY CP+LICMYIMGF+D  F PEHKKEM RYIYNHQNEDG W LH+G HS+MFCT  
Sbjct: 121 GPLFYNCPLLICMYIMGFLDAAFPPEHKKEMKRYIYNHQNEDGGWGLHVGGHSNMFCTTF 180

Query: 192 NYISLRLLGEGPDLEPLAIARNWIRHRGG-------------ILNVFEWCGSNPMPPECW 236
           NYISLRLLGEGPD+EP+A  RNW+R  GG             ILNVF+W  SNPMPPE W
Sbjct: 181 NYISLRLLGEGPDVEPVARGRNWVREHGGVTSILSWGKTWLSILNVFDWSASNPMPPEYW 240

BLAST of Lsi11G004940 vs. NCBI nr
Match: XP_016901493.1 (PREDICTED: LOW QUALITY PROTEIN: isomultiflorenol synthase-like [Cucumis melo])

HSP 1 Score: 406.4 bits (1043), Expect = 1.7e-109
Identity = 193/251 (76.89%), Postives = 205/251 (81.67%), Query Frame = 0

Query: 12  MWRLKVGDGGNDPYIYSVNNFVGRQIWEFDPNAGTPEERAE---------------FLNR 71
           MWRLKVG+ GNDPYIYSVNNFVGRQIWEFDPNAGTPEERAE               F + 
Sbjct: 1   MWRLKVGESGNDPYIYSVNNFVGRQIWEFDPNAGTPEERAEVERVRYNFTKNRRKGFPSA 60

Query: 72  EIIIWMQLLREKNFKQNIPRVKVKDGEEINYEMA-SNATRRGAYFLAAIQTSDGHWPSET 131
           ++   +Q LREK FKQ+IP+VKV+DGEEI+YE A SNA RRGA+FLAAIQ SDGHWPSET
Sbjct: 61  DLFWRLQFLREKKFKQSIPQVKVEDGEEISYEKASSNAMRRGAHFLAAIQASDGHWPSET 120

Query: 132 SGPLFYICPMLICMYIMGFMDKVFSPEHKKEMLRYIYNHQNEDGEWALHIGSHSSMFCTA 191
           SGPLFYICPMLIC+YIMG MDKVFSPEHKKEMLRYIYNHQNEDG WALHIGSHSSMFCT 
Sbjct: 121 SGPLFYICPMLICIYIMGCMDKVFSPEHKKEMLRYIYNHQNEDGGWALHIGSHSSMFCTT 180

Query: 192 LNYISLRLLGEGPDLEPLAIARNWIRHRGG-------------ILNVFEWCGSNPMPPEC 234
           LNYISLRLLGEGPD EPL IARNWIR RGG             ILNVF+WCGSNPMPPEC
Sbjct: 181 LNYISLRLLGEGPDYEPLVIARNWIRQRGGVTYIPSWGKTWLSILNVFDWCGSNPMPPEC 240

BLAST of Lsi11G004940 vs. NCBI nr
Match: XP_031737440.1 (isomultiflorenol synthase [Cucumis sativus])

HSP 1 Score: 389.0 bits (998), Expect = 2.9e-104
Identity = 178/254 (70.08%), Postives = 200/254 (78.74%), Query Frame = 0

Query: 12  MWRLKVGDGGNDPYIYSVNNFVGRQIWEFDPNAGTPEERAE-----------------FL 71
           MWRLKVGDGGNDPYIYS+NNFVGRQIWEFDPNAGTPEER E                 FL
Sbjct: 1   MWRLKVGDGGNDPYIYSMNNFVGRQIWEFDPNAGTPEERVEVERLRQNFINKRHKENSFL 60

Query: 72  NREIIIWMQLLREKNFKQNIPRVKVKDGEEINYEMASNATRRGAYFLAAIQTSDGHWPSE 131
           + +++  +Q LREK FKQ+IP+ KV+DGEEI+YE ASNA RRGAYFLAAIQ SDGHWPSE
Sbjct: 61  SADLLWRLQFLREKKFKQSIPQEKVEDGEEISYEKASNAMRRGAYFLAAIQASDGHWPSE 120

Query: 132 TSGPLFYICPMLICMYIMGFMDKVFSPEHKKEMLRYIYNHQNEDGEWALHIGSHSSMFCT 191
           TSGPLFY+CPMLIC+Y+MG MD + SPEHKKEMLRYIYNHQNEDG W LH+G HS+MFCT
Sbjct: 121 TSGPLFYMCPMLICIYVMGIMDTILSPEHKKEMLRYIYNHQNEDGGWGLHVGGHSNMFCT 180

Query: 192 ALNYISLRLLGEGPDLEPLAIARNWIRHRGG-------------ILNVFEWCGSNPMPPE 236
             NYISLRLLGEGP++E L  +RNWIRHRGG             ILNVF+W GSNPMPPE
Sbjct: 181 TFNYISLRLLGEGPEVEQLFRSRNWIRHRGGVTSIPSWGKTWLSILNVFDWSGSNPMPPE 240

BLAST of Lsi11G004940 vs. NCBI nr
Match: XP_038899142.1 (isomultiflorenol synthase [Benincasa hispida])

HSP 1 Score: 387.5 bits (994), Expect = 8.3e-104
Identity = 176/252 (69.84%), Postives = 199/252 (78.97%), Query Frame = 0

Query: 12  MWRLKVGDGGNDPYIYSVNNFVGRQIWEFDPNAGTPEERAE---------------FLNR 71
           MWRLKV DGGNDPYIYS+NNFVGRQIWEFDPNAGTPEERAE               F + 
Sbjct: 1   MWRLKVADGGNDPYIYSMNNFVGRQIWEFDPNAGTPEERAEVEHVRNEFTKNRLKGFPSA 60

Query: 72  EIIIWMQLLREKNFKQNIPRVKVKDGEEINYEMASNATRRGAYFLAAIQTSDGHWPSETS 131
           +++  +QLLREKNFKQ+IP VKV+DGEEI+YEMA +A RRGAYFLAAIQ SDGHWPSETS
Sbjct: 61  DLLWRLQLLREKNFKQSIPAVKVEDGEEISYEMAWDAMRRGAYFLAAIQASDGHWPSETS 120

Query: 132 GPLFYICPMLICMYIMGFMDKVFSPEHKKEMLRYIYNHQNEDGEWALHIGSHSSMFCTAL 191
           GPLFY+CP+LICMYIMGFMD  F+PEHKKEM+RY+YNHQNEDG W LH+G HS+MFCT  
Sbjct: 121 GPLFYLCPLLICMYIMGFMDTAFTPEHKKEMMRYVYNHQNEDGGWGLHVGGHSNMFCTTF 180

Query: 192 NYISLRLLGEGPDLEPLAIARNWIRHRGG-------------ILNVFEWCGSNPMPPECW 236
           NYISLRLLGEGPD+EP++  RNWIR  GG             ILNVF+W  SNPMPPE W
Sbjct: 181 NYISLRLLGEGPDVEPISRGRNWIRDHGGVTSILSWGKTWLSILNVFDWSASNPMPPEYW 240

BLAST of Lsi11G004940 vs. NCBI nr
Match: XP_011648794.1 (isomultiflorenol synthase isoform X1 [Cucumis sativus] >KGN60880.2 hypothetical protein Csa_023422 [Cucumis sativus])

HSP 1 Score: 383.6 bits (984), Expect = 1.2e-102
Identity = 174/252 (69.05%), Postives = 201/252 (79.76%), Query Frame = 0

Query: 12  MWRLKVGDGGNDPYIYSVNNFVGRQIWEFDPNAGTPEERAE---------------FLNR 71
           MWRLK+G+GGNDPYIYS+NNFVGRQIWEFDPNAGTPEE+AE               F + 
Sbjct: 1   MWRLKIGEGGNDPYIYSMNNFVGRQIWEFDPNAGTPEEQAEIEHLRQRFTKNHLKGFPSG 60

Query: 72  EIIIWMQLLREKNFKQNIPRVKVKDGEEINYEMASNATRRGAYFLAAIQTSDGHWPSETS 131
           +++  +Q LREK FKQ+IP+VKV+DGEEI+Y+ ASNA RRGAYFLAAIQ SDGHWPSE+S
Sbjct: 61  DLLWRLQFLREKKFKQSIPQVKVEDGEEISYDKASNAMRRGAYFLAAIQASDGHWPSESS 120

Query: 132 GPLFYICPMLICMYIMGFMDKVFSPEHKKEMLRYIYNHQNEDGEWALHIGSHSSMFCTAL 191
           GPLFY+CPMLICMYIMG MD + SPEHKKEMLRY+YNHQNEDG W LH+G HS+MFCT  
Sbjct: 121 GPLFYLCPMLICMYIMGTMDTILSPEHKKEMLRYVYNHQNEDGGWGLHVGGHSNMFCTTF 180

Query: 192 NYISLRLLGEGPDLEPLAIARNWIRHRGG-------------ILNVFEWCGSNPMPPECW 236
           NYISLRLLGEGP++E L+ +RNWIR RGG             ILNVF+W GSNPMPPE W
Sbjct: 181 NYISLRLLGEGPEVEQLSRSRNWIRQRGGVTSIPSWGKTWLSILNVFDWSGSNPMPPEYW 240

BLAST of Lsi11G004940 vs. NCBI nr
Match: KAE8651573.1 (hypothetical protein Csa_023449, partial [Cucumis sativus])

HSP 1 Score: 383.6 bits (984), Expect = 1.2e-102
Identity = 174/252 (69.05%), Postives = 201/252 (79.76%), Query Frame = 0

Query: 12  MWRLKVGDGGNDPYIYSVNNFVGRQIWEFDPNAGTPEERAE---------------FLNR 71
           MWRLK+G+GGNDPYIYS+NNFVGRQIWEFDPNAGTPEE+AE               F + 
Sbjct: 1   MWRLKIGEGGNDPYIYSMNNFVGRQIWEFDPNAGTPEEQAEIEHLRQRFTKNHLKGFPSG 60

Query: 72  EIIIWMQLLREKNFKQNIPRVKVKDGEEINYEMASNATRRGAYFLAAIQTSDGHWPSETS 131
           +++  +Q LREK FKQ+IP+VKV+DGEEI+Y+ ASNA RRGAYFLAAIQ SDGHWPSE+S
Sbjct: 61  DLLWRLQFLREKKFKQSIPQVKVEDGEEISYDKASNAMRRGAYFLAAIQASDGHWPSESS 120

Query: 132 GPLFYICPMLICMYIMGFMDKVFSPEHKKEMLRYIYNHQNEDGEWALHIGSHSSMFCTAL 191
           GPLFY+CPMLICMYIMG MD + SPEHKKEMLRY+YNHQNEDG W LH+G HS+MFCT  
Sbjct: 121 GPLFYLCPMLICMYIMGTMDTILSPEHKKEMLRYVYNHQNEDGGWGLHVGGHSNMFCTTF 180

Query: 192 NYISLRLLGEGPDLEPLAIARNWIRHRGG-------------ILNVFEWCGSNPMPPECW 236
           NYISLRLLGEGP++E L+ +RNWIR RGG             ILNVF+W GSNPMPPE W
Sbjct: 181 NYISLRLLGEGPEVEQLSRSRNWIRQRGGVTSIPSWGKTWLSILNVFDWSGSNPMPPEYW 240

BLAST of Lsi11G004940 vs. TAIR 10
Match: AT1G78950.1 (Terpenoid cyclases family protein )

HSP 1 Score: 283.1 bits (723), Expect = 2.1e-76
Identity = 132/254 (51.97%), Postives = 172/254 (67.72%), Query Frame = 0

Query: 12  MWRLKVGDG-GNDPYIYSVNNFVGRQIWEFDPNAGTPEERAEFLN-REI----------- 71
           MWRLK+G+G G+DPY+++ NNF GRQ WEFDP+ G+PEER   +  R I           
Sbjct: 1   MWRLKIGEGNGDDPYLFTTNNFAGRQTWEFDPDGGSPEERHSVVEARRIFYDNRFHVKAS 60

Query: 72  --IIW-MQLLREKNFKQNIPRVKVKDGEEINYEMASNATRRGAYFLAAIQTSDGHWPSET 131
             ++W MQ LREK F+Q I  VKV+D E++ +E A++A RRG +F +A+Q SDGHWP+E 
Sbjct: 61  SDLLWRMQFLREKKFEQRIAPVKVEDSEKVTFETATSALRRGIHFFSALQASDGHWPAEN 120

Query: 132 SGPLFYICPMLICMYIMGFMDKVFSPEHKKEMLRYIYNHQNEDGEWALHIGSHSSMFCTA 191
           +GPLF++ P++ C+YI G +D+VF+ EH+KE+LRYIY HQ EDG W LHI  HS+MFCT 
Sbjct: 121 AGPLFFLPPLVFCLYITGHLDEVFTSEHRKEILRYIYCHQKEDGGWGLHIEGHSTMFCTT 180

Query: 192 LNYISLRLLGEGPD---LEPLAIARNWIRHRGG-------------ILNVFEWCGSNPMP 234
           LNYI +R+LGE PD         AR WI   GG             IL VF+W GSNPMP
Sbjct: 181 LNYICMRILGESPDGGHDNACGRAREWILSHGGVTYIPSWGKTWLSILGVFDWSGSNPMP 240

BLAST of Lsi11G004940 vs. TAIR 10
Match: AT1G78970.2 (lupeol synthase 1 )

HSP 1 Score: 275.4 bits (703), Expect = 4.3e-74
Identity = 131/251 (52.19%), Postives = 167/251 (66.53%), Query Frame = 0

Query: 12  MWRLKVGDG-GNDPYIYSVNNFVGRQIWEFDPNAGTPEERAE-------FLNREI----- 71
           MW+LK+G G G DP+++S NNFVGRQ W+FD  AG+PEERA        FL+        
Sbjct: 1   MWKLKIGKGNGEDPHLFSSNNFVGRQTWKFDHKAGSPEERAAVEEARRGFLDNRFRVKGC 60

Query: 72  --IIW-MQLLREKNFKQNIPRVKVKDGEEINYEMASNATRRGAYFLAAIQTSDGHWPSET 131
             ++W MQ LREK F+Q IP++K  + EEI YE  +NA RRG  +  A+Q SDGHWP E 
Sbjct: 61  SDLLWRMQFLREKKFEQGIPQLKATNIEEITYETTTNALRRGVRYFTALQASDGHWPGEI 120

Query: 132 SGPLFYICPMLICMYIMGFMDKVFSPEHKKEMLRYIYNHQNEDGEWALHIGSHSSMFCTA 191
           +GPLF++ P++ C+YI G +++VF  EH+KEMLR+IY HQNEDG W LHI S S MFCT 
Sbjct: 121 TGPLFFLPPLIFCLYITGHLEEVFDAEHRKEMLRHIYCHQNEDGGWGLHIESKSVMFCTV 180

Query: 192 LNYISLRLLGEGPDLEPLAIARNWIRHRGG-------------ILNVFEWCGSNPMPPEC 234
           LNYI LR+LGE P+ +    AR WI  RGG             IL V++W G+NP PPE 
Sbjct: 181 LNYICLRMLGENPEQDACKRARQWILDRGGVIFIPSWGKFWLSILGVYDWSGTNPTPPEL 240

BLAST of Lsi11G004940 vs. TAIR 10
Match: AT1G78970.1 (lupeol synthase 1 )

HSP 1 Score: 275.4 bits (703), Expect = 4.3e-74
Identity = 131/251 (52.19%), Postives = 167/251 (66.53%), Query Frame = 0

Query: 12  MWRLKVGDG-GNDPYIYSVNNFVGRQIWEFDPNAGTPEERAE-------FLNREI----- 71
           MW+LK+G G G DP+++S NNFVGRQ W+FD  AG+PEERA        FL+        
Sbjct: 1   MWKLKIGKGNGEDPHLFSSNNFVGRQTWKFDHKAGSPEERAAVEEARRGFLDNRFRVKGC 60

Query: 72  --IIW-MQLLREKNFKQNIPRVKVKDGEEINYEMASNATRRGAYFLAAIQTSDGHWPSET 131
             ++W MQ LREK F+Q IP++K  + EEI YE  +NA RRG  +  A+Q SDGHWP E 
Sbjct: 61  SDLLWRMQFLREKKFEQGIPQLKATNIEEITYETTTNALRRGVRYFTALQASDGHWPGEI 120

Query: 132 SGPLFYICPMLICMYIMGFMDKVFSPEHKKEMLRYIYNHQNEDGEWALHIGSHSSMFCTA 191
           +GPLF++ P++ C+YI G +++VF  EH+KEMLR+IY HQNEDG W LHI S S MFCT 
Sbjct: 121 TGPLFFLPPLIFCLYITGHLEEVFDAEHRKEMLRHIYCHQNEDGGWGLHIESKSVMFCTV 180

Query: 192 LNYISLRLLGEGPDLEPLAIARNWIRHRGG-------------ILNVFEWCGSNPMPPEC 234
           LNYI LR+LGE P+ +    AR WI  RGG             IL V++W G+NP PPE 
Sbjct: 181 LNYICLRMLGENPEQDACKRARQWILDRGGVIFIPSWGKFWLSILGVYDWSGTNPTPPEL 240

BLAST of Lsi11G004940 vs. TAIR 10
Match: AT1G78960.1 (lupeol synthase 2 )

HSP 1 Score: 271.6 bits (693), Expect = 6.2e-73
Identity = 130/253 (51.38%), Postives = 167/253 (66.01%), Query Frame = 0

Query: 12  MWRLKVGDG-GNDPYIYSVNNFVGRQIWEFDPNAGTPEERA-------EFLNR------- 71
           MW+LK+G+G G DPY++S NNFVGRQ WEFDP AGTPEERA        +L+        
Sbjct: 1   MWKLKIGEGNGEDPYLFSSNNFVGRQTWEFDPKAGTPEERAAVEDARRNYLDNRPRVKGC 60

Query: 72  EIIIW-MQLLREKNFKQNIPRVKVKDGEEINYEMASNATRRGAYFLAAIQTSDGHWPSET 131
             ++W MQ L+E  F+Q IP VK+ DGE I Y+ A++A RR   F +A+Q+SDGHWP+E 
Sbjct: 61  SDLLWRMQFLKEAKFEQVIPPVKIDDGEGITYKNATDALRRAVSFYSALQSSDGHWPAEI 120

Query: 132 SGPLFYICPMLICMYIMGFMDKVFSPEHKKEMLRYIYNHQNEDGEWALHIGSHSSMFCTA 191
           +G LF++ P++ C YI G ++K+F  EH+KEMLR+IY HQNEDG W LHI   S MFCT 
Sbjct: 121 TGTLFFLPPLVFCFYITGHLEKIFDAEHRKEMLRHIYCHQNEDGGWGLHIEGKSVMFCTV 180

Query: 192 LNYISLRLLGEGPD---LEPLAIARNWIRHRGG-------------ILNVFEWCGSNPMP 233
           LNYI LR+LGEGP+         AR WI   GG             IL +++W G+NPMP
Sbjct: 181 LNYICLRMLGEGPNGGRNNACKRARQWILDHGGVTYIPSWGKIWLSILGIYDWSGTNPMP 240

BLAST of Lsi11G004940 vs. TAIR 10
Match: AT1G78955.1 (camelliol C synthase 1 )

HSP 1 Score: 270.8 bits (691), Expect = 1.1e-72
Identity = 127/254 (50.00%), Postives = 172/254 (67.72%), Query Frame = 0

Query: 12  MWRLKVGDGG-NDPYIYSVNNFVGRQIWEFDPNAGTPEE-------RAEFLNREI----- 71
           MW+LK+ +G   +PY++S NNF+GRQ WEFDP+AGT EE       R +F +        
Sbjct: 1   MWKLKIANGNKEEPYLFSTNNFLGRQTWEFDPDAGTVEELAAVEEARRKFYDDRFRVKAS 60

Query: 72  --IIW-MQLLREKNFKQNIPRVKVKDGEEINYEMASNATRRGAYFLAAIQTSDGHWPSET 131
             +IW MQ L+EK F+Q IP  KV+D   I  E+A+NA R+G  FL+A+Q SDGHWP+E 
Sbjct: 61  SDLIWRMQFLKEKKFEQVIPPAKVEDANNITSEIATNALRKGVNFLSALQASDGHWPAEN 120

Query: 132 SGPLFYICPMLICMYIMGFMDKVFSPEHKKEMLRYIYNHQNEDGEWALHIGSHSSMFCTA 191
           +GPLF++ P++ C+Y+ G + ++F+ +H++E+LRYIY HQNEDG W LHI  +S+MFCT 
Sbjct: 121 AGPLFFLPPLVFCLYVTGHLHEIFTQDHRREVLRYIYCHQNEDGGWGLHIEGNSTMFCTT 180

Query: 192 LNYISLRLLGEGPDLEP---LAIARNWIRHRGG-------------ILNVFEWCGSNPMP 234
           LNYI +R+LGEGP+  P      AR+WI   GG             IL VF+W GSNPMP
Sbjct: 181 LNYICMRILGEGPNGGPGNACKRARDWILDHGGATYIPSWGKTWLSILGVFDWSGSNPMP 240

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q948R67.8e-10569.84Isomultiflorenol synthase OS=Luffa aegyptiaca OX=3670 GN=IMS1 PE=1 SV=1[more]
Q9MB424.9e-8356.92Beta-amyrin synthase OS=Glycyrrhiza glabra OX=49827 GN=GgbAS1 PE=1 SV=1[more]
Q8W3Z12.4e-8256.92Beta-amyrin synthase OS=Betula platyphylla OX=78630 GN=OSCBPY PE=1 SV=1[more]
Q9LRH87.9e-8155.34Beta-amyrin synthase OS=Pisum sativum OX=3888 GN=OSCPSY PE=2 SV=1[more]
A8C9803.9e-8054.55Germanicol synthase OS=Rhizophora stylosa OX=98588 GN=M1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S4DZT58.4e-11076.89Terpene cyclase/mutase family member OS=Cucumis melo OX=3656 GN=LOC103495616 PE=... [more]
A0A0A0LFN01.4e-10470.08SQHop_cyclase_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G01... [more]
A0A0A0LG935.8e-10369.05Terpene cyclase/mutase family member OS=Cucumis sativus OX=3659 GN=Csa_2G013830 ... [more]
A0A5A7VDU04.9e-10269.05Isomultiflorenol synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s... [more]
A0A5D3BXV97.1e-10167.86H(+)-exporting diphosphatase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
Match NameE-valueIdentityDescription
XP_016901493.11.7e-10976.89PREDICTED: LOW QUALITY PROTEIN: isomultiflorenol synthase-like [Cucumis melo][more]
XP_031737440.12.9e-10470.08isomultiflorenol synthase [Cucumis sativus][more]
XP_038899142.18.3e-10469.84isomultiflorenol synthase [Benincasa hispida][more]
XP_011648794.11.2e-10269.05isomultiflorenol synthase isoform X1 [Cucumis sativus] >KGN60880.2 hypothetical ... [more]
KAE8651573.11.2e-10269.05hypothetical protein Csa_023449, partial [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT1G78950.12.1e-7651.97Terpenoid cyclases family protein [more]
AT1G78970.24.3e-7452.19lupeol synthase 1 [more]
AT1G78970.14.3e-7452.19lupeol synthase 1 [more]
AT1G78960.16.2e-7351.38lupeol synthase 2 [more]
AT1G78955.11.1e-7250.00camelliol C synthase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032697Squalene cyclase, N-terminalPFAMPF13249SQHop_cyclase_Ncoord: 98..233
e-value: 4.3E-21
score: 75.3
NoneNo IPR availableGENE3D1.50.10.20coord: 115..237
e-value: 6.5E-41
score: 142.3
NoneNo IPR availablePANTHERPTHR11764:SF58LUPEOL SYNTHASE 1coord: 12..234
IPR018333Squalene cyclasePANTHERPTHR11764TERPENE CYCLASE/MUTASE FAMILY MEMBERcoord: 12..234
IPR008930Terpenoid cyclases/protein prenyltransferase alpha-alpha toroidSUPERFAMILY48239Terpenoid cyclases/Protein prenyltransferasescoord: 36..233

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi11G004940.1Lsi11G004940.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006811 ion transport
biological_process GO:0016104 triterpenoid biosynthetic process
cellular_component GO:0005811 lipid droplet
cellular_component GO:0016020 membrane
molecular_function GO:0042300 beta-amyrin synthase activity
molecular_function GO:0000250 lanosterol synthase activity
molecular_function GO:0016866 intramolecular transferase activity