Lsi11G004020 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi11G004020
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionCBL-interacting serine/threonine-protein kinase 6
Locationchr11: 4196553 .. 4197854 (-)
RNA-Seq ExpressionLsi11G004020
SyntenyLsi11G004020
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGATAAAGGCAGAGACGGCGGCCACTCGAGTTTACTCGACGGGAAGTACGAATTAGGCCGTCTTCTAGGGCATGGAACCTTCGCCAAGGTTTACCATGCCCGGAATCTGCGAACGGGTAAGAGCGTCGCGATGAAAGTGGTTGGGAAGGAGAAGATCATCAAAGTCGGCATGACAGAACAGATCAAGAGAGAAATCTCTGTGATGAAAATGGTGAAGCATCCTAATATTGTGGAGCTTCATGAGGTGATGGCGAGTAAATCGAAGATCTATTTCGCGATGGAGCTTGTTAGAGGCGGTGAACTGTTCTCCAAAATCGCGAAAGGTCGGTTGCGAGAGGATGTGGCTAGAGTTTACTTTCAGCAATTAATCTCTGCAATCGATTTCTGCCATAGCCGTGGAGTTTACCACCGTGATCTGAAACCGGAAAATCTCCTACTCGACGAGGACGGTAACCTTAAAGTCACCGATTTCGGCCTCACCGCCTTCTCAGAGCACTTGAAGCAAGACGGATTGTTGCATACAACGTGCGGAACTCCGGCGTACGTCGCGCCGGAGGTCATTGGAAAGCAAGGATACGACGGCGCGAAGGCCGATCTGTGGTCTTGTGGAGTCATCCTTTATGTTCTTCTCGCCGGATACCTTCCATTTCAGGACGACAACATGGTGGCGATGTACCGGAAGATTTACAGAGGCGACTTCAAGTGTCCGTCGTGGTTCTCTCCCGAAGCCCGTCGGCTGATCACAAAGCTTCTAGATCCTAATCCGGTGAGCAGAGTCGGTATCGCGAAGATTACATCGTCATCGTGGTTCAAGAAATCGGTACCGAAGTCTCTGATTACGAAGGAGGAGCAAGAACAAACAGAGGAGAATTTGACGAAATCAAAGCCACCGGCGGAAACATTGAACGCGTTCCACATAATATCTCTATCGGAAGGGTTCGATCTGTCTCCGTTGTTCGAGGAGAAGAAGAGGGAGGAGAAAGAGGAGATGAGATTCGCGACTTCATCGCCGGCGAGCAGTGTGATATCGAAGCTTGAGGAAGTGGGGAAGGCAGTGAAGTTCGATGTGAAGAAGAGCGAATCGTGGGTTCGATTGCAGGGGCAAGAGAAAGGGAGAAAGGGGAAACTGACGATTGCGGCCGATATCTTTGCGATGACGCCGTCGTTTGTGGTGGTGGAGGTGAAGAAGGACAACGGAGATACTTTGGAGTATAACCAGTTCTGCCGGAAGGAGCTCCGGCCGGCCTTGAAGGACATTGTTTGGACTTCTCCGACGGGAAACTCGACCCCAGCTTGA

mRNA sequence

ATGGCGGATAAAGGCAGAGACGGCGGCCACTCGAGTTTACTCGACGGGAAGTACGAATTAGGCCGTCTTCTAGGGCATGGAACCTTCGCCAAGGTTTACCATGCCCGGAATCTGCGAACGGGTAAGAGCGTCGCGATGAAAGTGGTTGGGAAGGAGAAGATCATCAAAGTCGGCATGACAGAACAGATCAAGAGAGAAATCTCTGTGATGAAAATGGTGAAGCATCCTAATATTGTGGAGCTTCATGAGGTGATGGCGAGTAAATCGAAGATCTATTTCGCGATGGAGCTTGTTAGAGGCGGTGAACTGTTCTCCAAAATCGCGAAAGGTCGGTTGCGAGAGGATGTGGCTAGAGTTTACTTTCAGCAATTAATCTCTGCAATCGATTTCTGCCATAGCCGTGGAGTTTACCACCGTGATCTGAAACCGGAAAATCTCCTACTCGACGAGGACGGTAACCTTAAAGTCACCGATTTCGGCCTCACCGCCTTCTCAGAGCACTTGAAGCAAGACGGATTGTTGCATACAACGTGCGGAACTCCGGCGTACGTCGCGCCGGAGGTCATTGGAAAGCAAGGATACGACGGCGCGAAGGCCGATCTGTGGTCTTGTGGAGTCATCCTTTATGTTCTTCTCGCCGGATACCTTCCATTTCAGGACGACAACATGGTGGCGATGTACCGGAAGATTTACAGAGGCGACTTCAAGTGTCCGTCGTGGTTCTCTCCCGAAGCCCGTCGGCTGATCACAAAGCTTCTAGATCCTAATCCGGTGAGCAGAGTCGGTATCGCGAAGATTACATCGTCATCGTGGTTCAAGAAATCGGTACCGAAGTCTCTGATTACGAAGGAGGAGCAAGAACAAACAGAGGAGAATTTGACGAAATCAAAGCCACCGGCGGAAACATTGAACGCGTTCCACATAATATCTCTATCGGAAGGGTTCGATCTGTCTCCGTTGTTCGAGGAGAAGAAGAGGGAGGAGAAAGAGGAGATGAGATTCGCGACTTCATCGCCGGCGAGCAGTGTGATATCGAAGCTTGAGGAAGTGGGGAAGGCAGTGAAGTTCGATGTGAAGAAGAGCGAATCGTGGGTTCGATTGCAGGGGCAAGAGAAAGGGAGAAAGGGGAAACTGACGATTGCGGCCGATATCTTTGCGATGACGCCGTCGTTTGTGGTGGTGGAGGTGAAGAAGGACAACGGAGATACTTTGGAGTATAACCAGTTCTGCCGGAAGGAGCTCCGGCCGGCCTTGAAGGACATTGTTTGGACTTCTCCGACGGGAAACTCGACCCCAGCTTGA

Coding sequence (CDS)

ATGGCGGATAAAGGCAGAGACGGCGGCCACTCGAGTTTACTCGACGGGAAGTACGAATTAGGCCGTCTTCTAGGGCATGGAACCTTCGCCAAGGTTTACCATGCCCGGAATCTGCGAACGGGTAAGAGCGTCGCGATGAAAGTGGTTGGGAAGGAGAAGATCATCAAAGTCGGCATGACAGAACAGATCAAGAGAGAAATCTCTGTGATGAAAATGGTGAAGCATCCTAATATTGTGGAGCTTCATGAGGTGATGGCGAGTAAATCGAAGATCTATTTCGCGATGGAGCTTGTTAGAGGCGGTGAACTGTTCTCCAAAATCGCGAAAGGTCGGTTGCGAGAGGATGTGGCTAGAGTTTACTTTCAGCAATTAATCTCTGCAATCGATTTCTGCCATAGCCGTGGAGTTTACCACCGTGATCTGAAACCGGAAAATCTCCTACTCGACGAGGACGGTAACCTTAAAGTCACCGATTTCGGCCTCACCGCCTTCTCAGAGCACTTGAAGCAAGACGGATTGTTGCATACAACGTGCGGAACTCCGGCGTACGTCGCGCCGGAGGTCATTGGAAAGCAAGGATACGACGGCGCGAAGGCCGATCTGTGGTCTTGTGGAGTCATCCTTTATGTTCTTCTCGCCGGATACCTTCCATTTCAGGACGACAACATGGTGGCGATGTACCGGAAGATTTACAGAGGCGACTTCAAGTGTCCGTCGTGGTTCTCTCCCGAAGCCCGTCGGCTGATCACAAAGCTTCTAGATCCTAATCCGGTGAGCAGAGTCGGTATCGCGAAGATTACATCGTCATCGTGGTTCAAGAAATCGGTACCGAAGTCTCTGATTACGAAGGAGGAGCAAGAACAAACAGAGGAGAATTTGACGAAATCAAAGCCACCGGCGGAAACATTGAACGCGTTCCACATAATATCTCTATCGGAAGGGTTCGATCTGTCTCCGTTGTTCGAGGAGAAGAAGAGGGAGGAGAAAGAGGAGATGAGATTCGCGACTTCATCGCCGGCGAGCAGTGTGATATCGAAGCTTGAGGAAGTGGGGAAGGCAGTGAAGTTCGATGTGAAGAAGAGCGAATCGTGGGTTCGATTGCAGGGGCAAGAGAAAGGGAGAAAGGGGAAACTGACGATTGCGGCCGATATCTTTGCGATGACGCCGTCGTTTGTGGTGGTGGAGGTGAAGAAGGACAACGGAGATACTTTGGAGTATAACCAGTTCTGCCGGAAGGAGCTCCGGCCGGCCTTGAAGGACATTGTTTGGACTTCTCCGACGGGAAACTCGACCCCAGCTTGA

Protein sequence

MADKGRDGGHSSLLDGKYELGRLLGHGTFAKVYHARNLRTGKSVAMKVVGKEKIIKVGMTEQIKREISVMKMVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIAKGRLREDVARVYFQQLISAIDFCHSRGVYHRDLKPENLLLDEDGNLKVTDFGLTAFSEHLKQDGLLHTTCGTPAYVAPEVIGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFKCPSWFSPEARRLITKLLDPNPVSRVGIAKITSSSWFKKSVPKSLITKEEQEQTEENLTKSKPPAETLNAFHIISLSEGFDLSPLFEEKKREEKEEMRFATSSPASSVISKLEEVGKAVKFDVKKSESWVRLQGQEKGRKGKLTIAADIFAMTPSFVVVEVKKDNGDTLEYNQFCRKELRPALKDIVWTSPTGNSTPA
Homology
BLAST of Lsi11G004020 vs. ExPASy Swiss-Prot
Match: O65554 (CBL-interacting serine/threonine-protein kinase 6 OS=Arabidopsis thaliana OX=3702 GN=CIPK6 PE=1 SV=1)

HSP 1 Score: 669.5 bits (1726), Expect = 2.6e-191
Identity = 337/432 (78.01%), Postives = 375/432 (86.81%), Query Frame = 0

Query: 3   DKGRDGGHSS-LLDGKYELGRLLGHGTFAKVYHARNLRTGKSVAMKVVGKEKIIKVGMTE 62
           + G DGG S+ LL G+YELGRLLGHGTFAKVYHARN++TGKSVAMKVVGKEK++KVGM +
Sbjct: 8   ENGSDGGSSTGLLHGRYELGRLLGHGTFAKVYHARNIQTGKSVAMKVVGKEKVVKVGMVD 67

Query: 63  QIKREISVMKMVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIAKGRLREDVARVYF 122
           QIKREISVM+MVKHPNIVELHEVMASKSKIYFAMELVRGGELF+K+AKGRLREDVARVYF
Sbjct: 68  QIKREISVMRMVKHPNIVELHEVMASKSKIYFAMELVRGGELFAKVAKGRLREDVARVYF 127

Query: 123 QQLISAIDFCHSRGVYHRDLKPENLLLDEDGNLKVTDFGLTAFSEHLKQDGLLHTTCGTP 182
           QQLISA+DFCHSRGVYHRDLKPENLLLDE+GNLKVTDFGL+AF+EHLKQDGLLHTTCGTP
Sbjct: 128 QQLISAVDFCHSRGVYHRDLKPENLLLDEEGNLKVTDFGLSAFTEHLKQDGLLHTTCGTP 187

Query: 183 AYVAPEVIGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFKCPSWF 242
           AYVAPEVI K+GYDGAKADLWSCGVIL+VLLAGYLPFQDDN+V MYRKIYRGDFKCP W 
Sbjct: 188 AYVAPEVILKKGYDGAKADLWSCGVILFVLLAGYLPFQDDNLVNMYRKIYRGDFKCPGWL 247

Query: 243 SPEARRLITKLLDPNPVSRVGIAKITSSSWFKKSVPKS-------LITKEEQEQTEENLT 302
           S +ARRL+TKLLDPNP +R+ I K+  S WFKK   +S        IT  E E  +  + 
Sbjct: 248 SSDARRLVTKLLDPNPNTRITIEKVMDSPWFKKQATRSRNEPVAATITTTE-EDVDFLVH 307

Query: 303 KSKPPAETLNAFHIISLSEGFDLSPLFEEKKREEKEEMRFATSSPASSVISKLEEVGK-A 362
           KSK   ETLNAFHII+LSEGFDLSPLFEEKK+EEK EMRFATS PASSVIS LEE  +  
Sbjct: 308 KSKEETETLNAFHIIALSEGFDLSPLFEEKKKEEKREMRFATSRPASSVISSLEEAARVG 367

Query: 363 VKFDVKKSESWVRLQGQEKGRKGKLTIAADIFAMTPSFVVVEVKKDNGDTLEYNQFCRKE 422
            KFDV+KSES VR++G++ GRKGKL + A+IFA+ PSFVVVEVKKD+GDTLEYN FC   
Sbjct: 368 NKFDVRKSESRVRIEGKQNGRKGKLAVEAEIFAVAPSFVVVEVKKDHGDTLEYNNFCSTA 427

Query: 423 LRPALKDIVWTS 426
           LRPALKDI WTS
Sbjct: 428 LRPALKDIFWTS 438

BLAST of Lsi11G004020 vs. ExPASy Swiss-Prot
Match: Q9LWM4 (CBL-interacting protein kinase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=CIPK5 PE=2 SV=1)

HSP 1 Score: 553.5 bits (1425), Expect = 2.1e-156
Identity = 270/431 (62.65%), Postives = 338/431 (78.42%), Query Frame = 0

Query: 11  SSLLDGKYELGRLLGHGTFAKVYHARNLRTGKSVAMKVVGKEKIIKVGMTEQIKREISVM 70
           +S+L  +YELGR+LG GTFAKVYHARNL + +SVA+KV+ KEK+++VGM +QIKREIS+M
Sbjct: 5   ASILMNRYELGRMLGQGTFAKVYHARNLASNQSVAIKVIDKEKVLRVGMIDQIKREISIM 64

Query: 71  KMVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIAKGRLREDVARVYFQQLISAIDF 130
           ++V+HPNIV+LHEVMASKSKIYFAME VRGGELFS++A+GRL+ED AR YFQQLI A+DF
Sbjct: 65  RLVRHPNIVQLHEVMASKSKIYFAMEYVRGGELFSRVARGRLKEDAARKYFQQLIGAVDF 124

Query: 131 CHSRGVYHRDLKPENLLLDEDGNLKVTDFGLTAFSEHLKQDGLLHTTCGTPAYVAPEVIG 190
           CHSRGVYHRDLKPENLL+DE+GNLKV+DFGL+AF E  KQDGLLHTTCGTPAYVAPE+I 
Sbjct: 125 CHSRGVYHRDLKPENLLVDENGNLKVSDFGLSAFKECQKQDGLLHTTCGTPAYVAPEIIN 184

Query: 191 KQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFKCPSWFSPEARRLIT 250
           K+GYDGAKAD+WSCGVIL+VLLAGYLPF D N++ MYRKI +GD K P WF+ + RRL++
Sbjct: 185 KRGYDGAKADIWSCGVILFVLLAGYLPFHDSNLMEMYRKISKGDVKFPQWFTTDVRRLLS 244

Query: 251 KLLDPNPVSRVGIAKITSSSWFKKSVPKSLITKEEQEQT-------------EENLTKSK 310
           +LLDPNP  R+ + K+    WFKK    +++  +  E               ++N  K+K
Sbjct: 245 RLLDPNPNIRITVEKLVEHPWFKKGYKPAVMLSQPNESNNLKDVHTAFSADHKDNEGKAK 304

Query: 311 PPAE-----TLNAFHIISLSEGFDLSPLFEEKKREEKEEMRFATSSPASSVISKLEEVGK 370
            PA      +LNAF IISLS+GFDLS LFE  K E+K + RF T  PAS+++SKLE++ +
Sbjct: 305 EPASSLKPVSLNAFDIISLSKGFDLSGLFENDK-EQKADSRFMTQKPASAIVSKLEQIAE 364

Query: 371 AVKFDVKKSESWVRLQGQEKGRKGKLTIAADIFAMTPSFVVVEVKKDNGDTLEYNQFCRK 424
              F VKK +  V+LQG ++GRKG+L I A+IF +TPSF VVEVKK  GDTLEY +FC K
Sbjct: 365 TESFKVKKQDGLVKLQGSKEGRKGQLAIDAEIFEVTPSFFVVEVKKSAGDTLEYEKFCNK 424

BLAST of Lsi11G004020 vs. ExPASy Swiss-Prot
Match: Q5W736 (CBL-interacting protein kinase 18 OS=Oryza sativa subsp. japonica OX=39947 GN=CIPK18 PE=2 SV=1)

HSP 1 Score: 529.3 bits (1362), Expect = 4.2e-149
Identity = 262/438 (59.82%), Postives = 334/438 (76.26%), Query Frame = 0

Query: 10  HSSLLDGKYELGRLLGHGTFAKVYHARNLRTGKSVAMKVVGKEKIIKVGMTEQIKREISV 69
           + ++L  +YE+G+LLG GTFAKVYH RN+ T +SVA+KV+ K+KI KVG+ +QIKREISV
Sbjct: 7   NGNILLRRYEIGKLLGQGTFAKVYHGRNIVTSQSVAIKVIDKDKIFKVGLMDQIKREISV 66

Query: 70  MKMVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIAKGRLREDVARVYFQQLISAID 129
           MK+V+HPNIV+L+EVMA+KSKIYF +E V+GGELF+K+AKGRL+ED AR YFQQL+SA+D
Sbjct: 67  MKLVRHPNIVQLYEVMATKSKIYFVLEYVKGGELFNKVAKGRLKEDAARKYFQQLVSAVD 126

Query: 130 FCHSRGVYHRDLKPENLLLDEDGNLKVTDFGLTAFSEHLKQDGLLHTTCGTPAYVAPEVI 189
           FCHSRGVYHRDLKPENLL+DE+GNLK+TDFGL+A +E  +QDGLLHTTCGTPAYVAPEVI
Sbjct: 127 FCHSRGVYHRDLKPENLLVDENGNLKITDFGLSALAESRRQDGLLHTTCGTPAYVAPEVI 186

Query: 190 GKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFKCPSWFSPEARRLI 249
            ++GYDG K D WSCGVIL+VL+AGYLPFQD N++ MYRKI + +FKCP+WFS + R+L+
Sbjct: 187 SRKGYDGVKVDTWSCGVILFVLMAGYLPFQDSNLMEMYRKIGKAEFKCPAWFSSDVRKLV 246

Query: 250 TKLLDPNPVSRVGIAKITSSSWFKKSVPKSLITK-----------------------EEQ 309
           +++LDPNP SR+ I KI  + WFKK +   LI K                          
Sbjct: 247 SRILDPNPRSRMPITKIMETYWFKKGLDSKLILKNVETNEPVTALADVNVVFSSMGSSSS 306

Query: 310 EQTEENLTKSKPPAETLNAFHIISLSEGFDLSPLFEEKKREEKEEMRFATSSPASSVISK 369
           ++TEE     K     LNAF IISLSEGFDLS LFEE   ++K+E RF +S  AS++ISK
Sbjct: 307 KKTEEKQDAGK--LTNLNAFDIISLSEGFDLSGLFEE--TDKKKEARFTSSQSASAIISK 366

Query: 370 LEEVGKAVKFDVKKSESWV-RLQGQEKGRKGKLTIAADIFAMTPSFVVVEVKKDNGDTLE 424
           LE+V    K  VKK E  V +++G  +GRKG L I A+IF +TPSF +VE+KK+NGDTLE
Sbjct: 367 LEDVASCSKLTVKKKEGGVLKMEGASEGRKGVLAIDAEIFEVTPSFHLVEIKKNNGDTLE 426

BLAST of Lsi11G004020 vs. ExPASy Swiss-Prot
Match: Q6Z9F4 (CBL-interacting protein kinase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=CIPK6 PE=2 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 2.1e-148
Identity = 267/433 (61.66%), Postives = 332/433 (76.67%), Query Frame = 0

Query: 4   KGRDGGHSSLLDGKYELGRLLGHGTFAKVYHARNLRTGKSVAMKVVGKEKIIKVGMTEQI 63
           +G+ GG  ++L G+YE+GR+LGHG F +V+ AR+LRTG+SVA+KVV KEK+++ GM EQI
Sbjct: 10  EGKKGG-GTVLQGRYEMGRVLGHGNFGRVHVARDLRTGRSVAVKVVAKEKVVRAGMMEQI 69

Query: 64  KREISVMKMVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIAK-GRLREDVARVYFQ 123
           KREI+VMK V HPNIVELHEVMA++SKIY A+ELVRGGELF +I + GR+RED AR YF+
Sbjct: 70  KREIAVMKRVSHPNIVELHEVMATRSKIYLALELVRGGELFGRIVRLGRVREDAARHYFR 129

Query: 124 QLISAIDFCHSRGVYHRDLKPENLLLDEDGNLKVTDFGLTAFSEHLKQDGLLHTTCGTPA 183
           QL+SA+DFCHSRGVYHRDLKPENLLLDE GNLKV DFGL+A ++H + DGLLHT CGTP 
Sbjct: 130 QLVSAVDFCHSRGVYHRDLKPENLLLDEAGNLKVVDFGLSALADHARADGLLHTLCGTPG 189

Query: 184 YVAPEVIGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFKCPSWFS 243
           Y APEV+  +GYDGAKADLWSCGVILYVLLAG LPF DDN+V +YRK  RGD++CP+W S
Sbjct: 190 YAAPEVLRDKGYDGAKADLWSCGVILYVLLAGSLPFPDDNIVTLYRKAQRGDYRCPAWLS 249

Query: 244 PEARRLITKLLDPNPVSRVGIAKITSSSWFKKSVPKSLITKE------EQEQTEENLTKS 303
            +ARRLI +LLDPNP +R+ +A++  + WFKK+     ++ E      +    +E   K 
Sbjct: 250 TDARRLIPRLLDPNPTTRISVAQLVETPWFKKTSISRPVSIELPPAFADPAPAKEEAEKD 309

Query: 304 KPPAETLNAFHIISLSEGFDLSPLFEEKKREEKEE--MRFATSSPASSVISKLEEV---G 363
           +P  ETLNAFH+ISLSEGFDLSPLFE    + + +  M FAT  PAS VIS+LE V   G
Sbjct: 310 EP--ETLNAFHLISLSEGFDLSPLFEGDSAKGRRDGGMLFATREPASGVISRLEGVAARG 369

Query: 364 KAVKFDVKKSESWVRLQGQEK-GRKGKLTIAADIFAMTPSFVVVEVKKDNGDTLEYNQFC 423
                  K     VRL+G E+ G KG+L +AADIF++ PS +VV+VKKD GDTLEY  FC
Sbjct: 370 GGRMRVTKSGARGVRLEGAERGGAKGRLAVAADIFSVAPSVLVVDVKKDGGDTLEYRSFC 429

BLAST of Lsi11G004020 vs. ExPASy Swiss-Prot
Match: Q9FJ54 (CBL-interacting serine/threonine-protein kinase 20 OS=Arabidopsis thaliana OX=3702 GN=CIPK20 PE=1 SV=1)

HSP 1 Score: 523.1 bits (1346), Expect = 3.0e-147
Identity = 253/414 (61.11%), Postives = 329/414 (79.47%), Query Frame = 0

Query: 17  KYELGRLLGHGTFAKVYHARNLRTGKSVAMKVVGKEKIIKVGMTEQIKREISVMKMVKHP 76
           KYELGRLLG GTFAKVYHARN++TG+SVA+KV+ K+K+ KVG+ +QIKREISVM++V+HP
Sbjct: 11  KYELGRLLGQGTFAKVYHARNIKTGESVAIKVIDKQKVAKVGLIDQIKREISVMRLVRHP 70

Query: 77  NIVELHEVMASKSKIYFAMELVRGGELFSKIAKGRLREDVARVYFQQLISAIDFCHSRGV 136
           ++V LHEVMASK+KIYFAME V+GGELF K++KG+L+E++AR YFQQLI AID+CHSRGV
Sbjct: 71  HVVFLHEVMASKTKIYFAMEYVKGGELFDKVSKGKLKENIARKYFQQLIGAIDYCHSRGV 130

Query: 137 YHRDLKPENLLLDEDGNLKVTDFGLTAFSEHLKQDGLLHTTCGTPAYVAPEVIGKQGYDG 196
           YHRDLKPENLLLDE+G+LK++DFGL+A  E  +QDGLLHTTCGTPAYVAPEVIGK+GYDG
Sbjct: 131 YHRDLKPENLLLDENGDLKISDFGLSALRESKQQDGLLHTTCGTPAYVAPEVIGKKGYDG 190

Query: 197 AKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFKCPSWFSPEARRLITKLLDPN 256
           AKAD+WSCGV+LYVLLAG+LPF + N+V MYRKI +G+FKCP+WF PE ++L++++LDPN
Sbjct: 191 AKADVWSCGVVLYVLLAGFLPFHEQNLVEMYRKITKGEFKCPNWFPPEVKKLLSRILDPN 250

Query: 257 PVSRVGIAKITSSSWFKKSVPKSLITKEEQEQTEENLTKSKPPAETL-----NAFHII-S 316
           P SR+ I KI  +SWF+K   K    K  +    ++L      A ++     NAF +I S
Sbjct: 251 PNSRIKIEKIMENSWFQKGFKKIETPKSPESHQIDSLISDVHAAFSVKPMSYNAFDLISS 310

Query: 317 LSEGFDLSPLFEEKKREEKEEMRFATSSPASSVISKLEEVG-KAVKFDVKKSESWVRLQG 376
           LS+GFDLS LFE   +EE+ E +F T   A  ++SK EE+   + +F++ KS+  V+++ 
Sbjct: 311 LSQGFDLSGLFE---KEERSESKFTTKKDAKEIVSKFEEIATSSERFNLTKSDVGVKMED 370

Query: 377 QEKGRKGKLTIAADIFAMTPSFVVVEVKKDNGDTLEYNQFCRKELRPALKDIVW 424
           + +GRKG L I  +IF +T SF +VE KK  GDT+EY QFC +ELRP+LKDIVW
Sbjct: 371 KREGRKGHLAIDVEIFEVTNSFHMVEFKKSGGDTMEYKQFCDRELRPSLKDIVW 421

BLAST of Lsi11G004020 vs. ExPASy TrEMBL
Match: A0A0A0LL73 (Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_2G009490 PE=3 SV=1)

HSP 1 Score: 843.6 bits (2178), Expect = 3.7e-241
Identity = 421/433 (97.23%), Postives = 425/433 (98.15%), Query Frame = 0

Query: 1   MADKGRDGGHSSLLDGKYELGRLLGHGTFAKVYHARNLRTGKSVAMKVVGKEKIIKVGMT 60
           MADKGRDGGHSSLLDGKYELGRLLGHGTFAKVYHARNLRTGKSVAMKVVGKEKIIKVGM 
Sbjct: 1   MADKGRDGGHSSLLDGKYELGRLLGHGTFAKVYHARNLRTGKSVAMKVVGKEKIIKVGMM 60

Query: 61  EQIKREISVMKMVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIAKGRLREDVARVY 120
           EQIKREISVMKMVKHPNIVELHEVMASKSKIYFAME VRGGELFSKIAKGRLREDVARVY
Sbjct: 61  EQIKREISVMKMVKHPNIVELHEVMASKSKIYFAMEFVRGGELFSKIAKGRLREDVARVY 120

Query: 121 FQQLISAIDFCHSRGVYHRDLKPENLLLDEDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT 180
           FQQLISAIDFCHSRGVYHRDLKPENLLLDEDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT
Sbjct: 121 FQQLISAIDFCHSRGVYHRDLKPENLLLDEDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT 180

Query: 181 PAYVAPEVIGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFKCPSW 240
           PAYVAPEVIGK GYDGAKADLWSCGVILYVLLAG+LPFQDDNMVAMYRKIYRGDFKCPSW
Sbjct: 181 PAYVAPEVIGKHGYDGAKADLWSCGVILYVLLAGFLPFQDDNMVAMYRKIYRGDFKCPSW 240

Query: 241 FSPEARRLITKLLDPNPVSRVGIAKITSSSWFKKSVPKSLITKEEQEQTEENLTKSKPPA 300
           FSPEARRLITKLLDPNPV+R GI KITSSSWFKKSVPKSLITK+EQEQTEEN TKSKPPA
Sbjct: 241 FSPEARRLITKLLDPNPVTRAGITKITSSSWFKKSVPKSLITKDEQEQTEENFTKSKPPA 300

Query: 301 ETLNAFHIISLSEGFDLSPLFEEKKREEKEEMRFATSSPASSVISKLEEVGKAVKFDVKK 360
           ETLNAFHIISLSEGFDLSPLFEEKKREEKEEMRFATSS ASSVISKLEEVGKAVKFDVKK
Sbjct: 301 ETLNAFHIISLSEGFDLSPLFEEKKREEKEEMRFATSSTASSVISKLEEVGKAVKFDVKK 360

Query: 361 SESWVRLQGQEKGRKGKLTIAADIFAMTPSFVVVEVKKDNGDTLEYNQFCRKELRPALKD 420
           SE+WVRLQGQE GRKGKLTIAADIFAMTPSFVVVEVKKDNGDTLEYNQFCRKELRPALKD
Sbjct: 361 SETWVRLQGQEMGRKGKLTIAADIFAMTPSFVVVEVKKDNGDTLEYNQFCRKELRPALKD 420

Query: 421 IVWTSPTGNSTPA 434
           IVWTSPTGNSTPA
Sbjct: 421 IVWTSPTGNSTPA 433

BLAST of Lsi11G004020 vs. ExPASy TrEMBL
Match: A0A5D3BFR3 (Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold285G001040 PE=3 SV=1)

HSP 1 Score: 833.9 bits (2153), Expect = 3.0e-238
Identity = 418/433 (96.54%), Postives = 423/433 (97.69%), Query Frame = 0

Query: 1   MADKGRDGGHSSLLDGKYELGRLLGHGTFAKVYHARNLRTGKSVAMKVVGKEKIIKVGMT 60
           MADKGRDGG+SSLLDGKYELGRLLGHGTFAKVYHARNLRTGKSVAMKVVGKEKIIKVGMT
Sbjct: 1   MADKGRDGGYSSLLDGKYELGRLLGHGTFAKVYHARNLRTGKSVAMKVVGKEKIIKVGMT 60

Query: 61  EQIKREISVMKMVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIAKGRLREDVARVY 120
           EQIKREISVMKMVKHPNIVELHEVMASKSKIYFAME VRGGELFSKIAKGRLREDVARVY
Sbjct: 61  EQIKREISVMKMVKHPNIVELHEVMASKSKIYFAMEFVRGGELFSKIAKGRLREDVARVY 120

Query: 121 FQQLISAIDFCHSRGVYHRDLKPENLLLDEDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT 180
           FQQLISAIDFCHSRGVYHRDLKPENLLLDEDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT
Sbjct: 121 FQQLISAIDFCHSRGVYHRDLKPENLLLDEDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT 180

Query: 181 PAYVAPEVIGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFKCPSW 240
           PAYVAPEVIGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFKCP W
Sbjct: 181 PAYVAPEVIGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFKCPPW 240

Query: 241 FSPEARRLITKLLDPNPVSRVGIAKITSSSWFKKSVPKSLITKEEQEQTEENLTKSKPPA 300
           FSPEARRLITKLLDPNPV+R GI KITSSSWFKKSVPK+LI K+EQEQTEEN TKSKPPA
Sbjct: 241 FSPEARRLITKLLDPNPVTRAGITKITSSSWFKKSVPKTLIRKDEQEQTEENFTKSKPPA 300

Query: 301 ETLNAFHIISLSEGFDLSPLFEEKKREEKEEMRFATSSPASSVISKLEEVGKAVKFDVKK 360
           ETLNAFHIISLSEGFDLSPLFEEKKREEKEEMRFATSS ASSVISKLEEV KAVKFDVKK
Sbjct: 301 ETLNAFHIISLSEGFDLSPLFEEKKREEKEEMRFATSSTASSVISKLEEVAKAVKFDVKK 360

Query: 361 SESWVRLQGQEKGRKGKLTIAADIFAMTPSFVVVEVKKDNGDTLEYNQFCRKELRPALKD 420
           SE+ VRLQGQE GRKGKLTIAADIFAMTPSFVVVEVKKDNGDTLEYNQFCRKELRPALKD
Sbjct: 361 SETRVRLQGQEMGRKGKLTIAADIFAMTPSFVVVEVKKDNGDTLEYNQFCRKELRPALKD 420

Query: 421 IVWTSPTGNSTPA 434
           IVWTSPTGNSTPA
Sbjct: 421 IVWTSPTGNSTPA 433

BLAST of Lsi11G004020 vs. ExPASy TrEMBL
Match: A0A6J1HPX1 (Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111465554 PE=3 SV=1)

HSP 1 Score: 831.6 bits (2147), Expect = 1.5e-237
Identity = 412/433 (95.15%), Postives = 424/433 (97.92%), Query Frame = 0

Query: 1   MADKGRDGGHSSLLDGKYELGRLLGHGTFAKVYHARNLRTGKSVAMKVVGKEKIIKVGMT 60
           MADKGRDGG+SSLLDGKYELGRLLGHGTFAKVYHARNLRTGK+VAMKVVGKEKIIKVGMT
Sbjct: 1   MADKGRDGGYSSLLDGKYELGRLLGHGTFAKVYHARNLRTGKTVAMKVVGKEKIIKVGMT 60

Query: 61  EQIKREISVMKMVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIAKGRLREDVARVY 120
           EQIKREISVMKMVKHPNIVELHEVMASKSKIYFAME VRGGELF+KIAKGRLREDVARVY
Sbjct: 61  EQIKREISVMKMVKHPNIVELHEVMASKSKIYFAMEFVRGGELFTKIAKGRLREDVARVY 120

Query: 121 FQQLISAIDFCHSRGVYHRDLKPENLLLDEDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT 180
           FQQLISAIDFCHSRGVYHRDLKPENLLLDEDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT
Sbjct: 121 FQQLISAIDFCHSRGVYHRDLKPENLLLDEDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT 180

Query: 181 PAYVAPEVIGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFKCPSW 240
           PAYVAPEVIGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMY+KIYRGDFKCP W
Sbjct: 181 PAYVAPEVIGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYKKIYRGDFKCPPW 240

Query: 241 FSPEARRLITKLLDPNPVSRVGIAKITSSSWFKKSVPKSLITKEEQEQTEENLTKSKPPA 300
           FS EARRL+TKLLDPNPV+R+GI KITSSSWFKKSVPK++ITKEEQE+ EEN TKSK PA
Sbjct: 241 FSSEARRLVTKLLDPNPVTRIGITKITSSSWFKKSVPKAIITKEEQERIEENFTKSKQPA 300

Query: 301 ETLNAFHIISLSEGFDLSPLFEEKKREEKEEMRFATSSPASSVISKLEEVGKAVKFDVKK 360
           ETLNAFHIISLSEGFDLSPLFEEKKRE+KEEMRFATSSPASSVISKLEEV KAVKFDVKK
Sbjct: 301 ETLNAFHIISLSEGFDLSPLFEEKKREDKEEMRFATSSPASSVISKLEEVAKAVKFDVKK 360

Query: 361 SESWVRLQGQEKGRKGKLTIAADIFAMTPSFVVVEVKKDNGDTLEYNQFCRKELRPALKD 420
           SESWVRLQGQEKGRKGKL IAADIFA+TPSFVVVEVKKDNGDTLEYNQFCRKELRPALKD
Sbjct: 361 SESWVRLQGQEKGRKGKLAIAADIFALTPSFVVVEVKKDNGDTLEYNQFCRKELRPALKD 420

Query: 421 IVWTSPTGNSTPA 434
           IVWTSPTGNSTPA
Sbjct: 421 IVWTSPTGNSTPA 433

BLAST of Lsi11G004020 vs. ExPASy TrEMBL
Match: A0A6J1FDM5 (Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111444420 PE=3 SV=1)

HSP 1 Score: 829.7 bits (2142), Expect = 5.6e-237
Identity = 412/433 (95.15%), Postives = 422/433 (97.46%), Query Frame = 0

Query: 1   MADKGRDGGHSSLLDGKYELGRLLGHGTFAKVYHARNLRTGKSVAMKVVGKEKIIKVGMT 60
           MADKGRDGGHSSLLDGKYELGRLLGHGTFAKVYHARNLRTGK+VAMKVVGKEKII+VGMT
Sbjct: 1   MADKGRDGGHSSLLDGKYELGRLLGHGTFAKVYHARNLRTGKTVAMKVVGKEKIIRVGMT 60

Query: 61  EQIKREISVMKMVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIAKGRLREDVARVY 120
           EQIKREISVMKMVKHPNIVELHEVMASKSKIYFAME VRGGELF+KIAKGRLREDVARVY
Sbjct: 61  EQIKREISVMKMVKHPNIVELHEVMASKSKIYFAMEFVRGGELFTKIAKGRLREDVARVY 120

Query: 121 FQQLISAIDFCHSRGVYHRDLKPENLLLDEDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT 180
           FQQLISAIDFCHSRGVYHRDLKPENLLLDEDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT
Sbjct: 121 FQQLISAIDFCHSRGVYHRDLKPENLLLDEDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT 180

Query: 181 PAYVAPEVIGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFKCPSW 240
           PAYVAPEVIGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMY+KIYRGDFKCP W
Sbjct: 181 PAYVAPEVIGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYKKIYRGDFKCPPW 240

Query: 241 FSPEARRLITKLLDPNPVSRVGIAKITSSSWFKKSVPKSLITKEEQEQTEENLTKSKPPA 300
           FS EARRL+TKLLDPNPV+R+GI KITSSSWFKKSVPKS+ITKEEQE  EEN TKSK PA
Sbjct: 241 FSSEARRLVTKLLDPNPVTRIGITKITSSSWFKKSVPKSIITKEEQEGIEENFTKSKQPA 300

Query: 301 ETLNAFHIISLSEGFDLSPLFEEKKREEKEEMRFATSSPASSVISKLEEVGKAVKFDVKK 360
           ETLNAFHIISLSEGFDLSPLFEEKKRE+KEEMRFATSSPASSVISKLEEV KAVKFDVKK
Sbjct: 301 ETLNAFHIISLSEGFDLSPLFEEKKREDKEEMRFATSSPASSVISKLEEVAKAVKFDVKK 360

Query: 361 SESWVRLQGQEKGRKGKLTIAADIFAMTPSFVVVEVKKDNGDTLEYNQFCRKELRPALKD 420
           SESWVRLQGQEKGRKGKL IAADIFA+TPSFVVVEVKKDNGDTLEYNQFCRKELRPALKD
Sbjct: 361 SESWVRLQGQEKGRKGKLAIAADIFALTPSFVVVEVKKDNGDTLEYNQFCRKELRPALKD 420

Query: 421 IVWTSPTGNSTPA 434
           IVWTSPT NSTPA
Sbjct: 421 IVWTSPTENSTPA 433

BLAST of Lsi11G004020 vs. ExPASy TrEMBL
Match: A0A6J1KAP0 (Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111493756 PE=3 SV=1)

HSP 1 Score: 814.7 bits (2103), Expect = 1.9e-232
Identity = 406/433 (93.76%), Postives = 420/433 (97.00%), Query Frame = 0

Query: 1   MADKGRDGGHSSLLDGKYELGRLLGHGTFAKVYHARNLRTGKSVAMKVVGKEKIIKVGMT 60
           MA+KGRDG HSSLLDGKYE GRLLGHGTFAKVYHARNL+TGKSVAMKVVGKEKIIK+GM 
Sbjct: 1   MANKGRDGEHSSLLDGKYEFGRLLGHGTFAKVYHARNLQTGKSVAMKVVGKEKIIKIGMM 60

Query: 61  EQIKREISVMKMVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIAKGRLREDVARVY 120
           EQIKREISVMKMVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKI KGRLREDVARVY
Sbjct: 61  EQIKREISVMKMVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIVKGRLREDVARVY 120

Query: 121 FQQLISAIDFCHSRGVYHRDLKPENLLLDEDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT 180
           FQQLISAIDFCHSRGVYHRDLKPENLLLD+DGNLKVTDFGLTAFSEHLKQDGLLHTTCGT
Sbjct: 121 FQQLISAIDFCHSRGVYHRDLKPENLLLDQDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT 180

Query: 181 PAYVAPEVIGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFKCPSW 240
           PAYVAPEV+GKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDF+CP W
Sbjct: 181 PAYVAPEVMGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFRCPPW 240

Query: 241 FSPEARRLITKLLDPNPVSRVGIAKITSSSWFKKSVPKSLITKEEQEQTEENLTKSKPPA 300
           FSPEARRL+TKLLDPNPV+RVGIAKITSS WFKKSVPK++ITK EQE+ EENL KS  PA
Sbjct: 241 FSPEARRLVTKLLDPNPVTRVGIAKITSSLWFKKSVPKTVITK-EQEEMEENLAKSNQPA 300

Query: 301 ETLNAFHIISLSEGFDLSPLFEEKKREEKEEMRFATSSPASSVISKLEEVGKAVKFDVKK 360
           ETLNAFHIISLSEGFDLSPLFEEKKREEKEEMRFATSSPASSVISKLEEV KAVKFDV+K
Sbjct: 301 ETLNAFHIISLSEGFDLSPLFEEKKREEKEEMRFATSSPASSVISKLEEVAKAVKFDVRK 360

Query: 361 SESWVRLQGQEKGRKGKLTIAADIFAMTPSFVVVEVKKDNGDTLEYNQFCRKELRPALKD 420
           SESWVRLQGQEKGRKGKL IAADIFAMTPSFVVVEVKKDNGDTLEYNQFCR+ELRPALKD
Sbjct: 361 SESWVRLQGQEKGRKGKLAIAADIFAMTPSFVVVEVKKDNGDTLEYNQFCREELRPALKD 420

Query: 421 IVWTSPTGNSTPA 434
           IVWTSPTG+STPA
Sbjct: 421 IVWTSPTGSSTPA 432

BLAST of Lsi11G004020 vs. NCBI nr
Match: XP_038890150.1 (CBL-interacting serine/threonine-protein kinase 6-like [Benincasa hispida])

HSP 1 Score: 849.0 bits (2192), Expect = 1.8e-242
Identity = 423/433 (97.69%), Postives = 428/433 (98.85%), Query Frame = 0

Query: 1   MADKGRDGGHSSLLDGKYELGRLLGHGTFAKVYHARNLRTGKSVAMKVVGKEKIIKVGMT 60
           MADKGRDGGHSSLLDGKYELGRLLGHGTFAKVYHARNLRTGKSVAMKVVGKEKIIKVGMT
Sbjct: 1   MADKGRDGGHSSLLDGKYELGRLLGHGTFAKVYHARNLRTGKSVAMKVVGKEKIIKVGMT 60

Query: 61  EQIKREISVMKMVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIAKGRLREDVARVY 120
           EQIKREISVMKMVKHPNIVELHEVMASKSKIYFAME VRGGELFSKIAKGRLREDVARVY
Sbjct: 61  EQIKREISVMKMVKHPNIVELHEVMASKSKIYFAMEFVRGGELFSKIAKGRLREDVARVY 120

Query: 121 FQQLISAIDFCHSRGVYHRDLKPENLLLDEDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT 180
           FQQLISAIDFCHSRGVYHRDLKPENLLLDEDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT
Sbjct: 121 FQQLISAIDFCHSRGVYHRDLKPENLLLDEDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT 180

Query: 181 PAYVAPEVIGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFKCPSW 240
           PAYVAPEVIGKQGYDGAKADLWSCGVIL+VLLAGYLPFQDDN+VAMY+KIYRGDFKCP W
Sbjct: 181 PAYVAPEVIGKQGYDGAKADLWSCGVILFVLLAGYLPFQDDNIVAMYKKIYRGDFKCPPW 240

Query: 241 FSPEARRLITKLLDPNPVSRVGIAKITSSSWFKKSVPKSLITKEEQEQTEENLTKSKPPA 300
           FSPEARRLITKLLDPNPVSR+GI KITSSSWFKKSVPK+LI KEEQEQTEENLTKSKPPA
Sbjct: 241 FSPEARRLITKLLDPNPVSRIGIGKITSSSWFKKSVPKTLIRKEEQEQTEENLTKSKPPA 300

Query: 301 ETLNAFHIISLSEGFDLSPLFEEKKREEKEEMRFATSSPASSVISKLEEVGKAVKFDVKK 360
           ETLNAFHIISLSEGFDLSPLFEEKKREEKEEMRFATSSPASSVISKLEEV KAVKFDVKK
Sbjct: 301 ETLNAFHIISLSEGFDLSPLFEEKKREEKEEMRFATSSPASSVISKLEEVAKAVKFDVKK 360

Query: 361 SESWVRLQGQEKGRKGKLTIAADIFAMTPSFVVVEVKKDNGDTLEYNQFCRKELRPALKD 420
           SESWVRLQGQEKGRKGKLTIAADIFAMTPSFVVVEVKKDNGDTLEYNQFCRKELRPALKD
Sbjct: 361 SESWVRLQGQEKGRKGKLTIAADIFAMTPSFVVVEVKKDNGDTLEYNQFCRKELRPALKD 420

Query: 421 IVWTSPTGNSTPA 434
           IVWTSPTGNSTPA
Sbjct: 421 IVWTSPTGNSTPA 433

BLAST of Lsi11G004020 vs. NCBI nr
Match: XP_004139258.1 (CBL-interacting serine/threonine-protein kinase 6 [Cucumis sativus] >KGN60761.1 hypothetical protein Csa_019503 [Cucumis sativus])

HSP 1 Score: 843.6 bits (2178), Expect = 7.7e-241
Identity = 421/433 (97.23%), Postives = 425/433 (98.15%), Query Frame = 0

Query: 1   MADKGRDGGHSSLLDGKYELGRLLGHGTFAKVYHARNLRTGKSVAMKVVGKEKIIKVGMT 60
           MADKGRDGGHSSLLDGKYELGRLLGHGTFAKVYHARNLRTGKSVAMKVVGKEKIIKVGM 
Sbjct: 1   MADKGRDGGHSSLLDGKYELGRLLGHGTFAKVYHARNLRTGKSVAMKVVGKEKIIKVGMM 60

Query: 61  EQIKREISVMKMVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIAKGRLREDVARVY 120
           EQIKREISVMKMVKHPNIVELHEVMASKSKIYFAME VRGGELFSKIAKGRLREDVARVY
Sbjct: 61  EQIKREISVMKMVKHPNIVELHEVMASKSKIYFAMEFVRGGELFSKIAKGRLREDVARVY 120

Query: 121 FQQLISAIDFCHSRGVYHRDLKPENLLLDEDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT 180
           FQQLISAIDFCHSRGVYHRDLKPENLLLDEDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT
Sbjct: 121 FQQLISAIDFCHSRGVYHRDLKPENLLLDEDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT 180

Query: 181 PAYVAPEVIGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFKCPSW 240
           PAYVAPEVIGK GYDGAKADLWSCGVILYVLLAG+LPFQDDNMVAMYRKIYRGDFKCPSW
Sbjct: 181 PAYVAPEVIGKHGYDGAKADLWSCGVILYVLLAGFLPFQDDNMVAMYRKIYRGDFKCPSW 240

Query: 241 FSPEARRLITKLLDPNPVSRVGIAKITSSSWFKKSVPKSLITKEEQEQTEENLTKSKPPA 300
           FSPEARRLITKLLDPNPV+R GI KITSSSWFKKSVPKSLITK+EQEQTEEN TKSKPPA
Sbjct: 241 FSPEARRLITKLLDPNPVTRAGITKITSSSWFKKSVPKSLITKDEQEQTEENFTKSKPPA 300

Query: 301 ETLNAFHIISLSEGFDLSPLFEEKKREEKEEMRFATSSPASSVISKLEEVGKAVKFDVKK 360
           ETLNAFHIISLSEGFDLSPLFEEKKREEKEEMRFATSS ASSVISKLEEVGKAVKFDVKK
Sbjct: 301 ETLNAFHIISLSEGFDLSPLFEEKKREEKEEMRFATSSTASSVISKLEEVGKAVKFDVKK 360

Query: 361 SESWVRLQGQEKGRKGKLTIAADIFAMTPSFVVVEVKKDNGDTLEYNQFCRKELRPALKD 420
           SE+WVRLQGQE GRKGKLTIAADIFAMTPSFVVVEVKKDNGDTLEYNQFCRKELRPALKD
Sbjct: 361 SETWVRLQGQEMGRKGKLTIAADIFAMTPSFVVVEVKKDNGDTLEYNQFCRKELRPALKD 420

Query: 421 IVWTSPTGNSTPA 434
           IVWTSPTGNSTPA
Sbjct: 421 IVWTSPTGNSTPA 433

BLAST of Lsi11G004020 vs. NCBI nr
Match: KAA0063563.1 (CBL-interacting serine/threonine-protein kinase 6-like [Cucumis melo var. makuwa] >TYJ97889.1 CBL-interacting serine/threonine-protein kinase 6-like [Cucumis melo var. makuwa])

HSP 1 Score: 833.9 bits (2153), Expect = 6.1e-238
Identity = 418/433 (96.54%), Postives = 423/433 (97.69%), Query Frame = 0

Query: 1   MADKGRDGGHSSLLDGKYELGRLLGHGTFAKVYHARNLRTGKSVAMKVVGKEKIIKVGMT 60
           MADKGRDGG+SSLLDGKYELGRLLGHGTFAKVYHARNLRTGKSVAMKVVGKEKIIKVGMT
Sbjct: 1   MADKGRDGGYSSLLDGKYELGRLLGHGTFAKVYHARNLRTGKSVAMKVVGKEKIIKVGMT 60

Query: 61  EQIKREISVMKMVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIAKGRLREDVARVY 120
           EQIKREISVMKMVKHPNIVELHEVMASKSKIYFAME VRGGELFSKIAKGRLREDVARVY
Sbjct: 61  EQIKREISVMKMVKHPNIVELHEVMASKSKIYFAMEFVRGGELFSKIAKGRLREDVARVY 120

Query: 121 FQQLISAIDFCHSRGVYHRDLKPENLLLDEDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT 180
           FQQLISAIDFCHSRGVYHRDLKPENLLLDEDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT
Sbjct: 121 FQQLISAIDFCHSRGVYHRDLKPENLLLDEDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT 180

Query: 181 PAYVAPEVIGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFKCPSW 240
           PAYVAPEVIGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFKCP W
Sbjct: 181 PAYVAPEVIGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFKCPPW 240

Query: 241 FSPEARRLITKLLDPNPVSRVGIAKITSSSWFKKSVPKSLITKEEQEQTEENLTKSKPPA 300
           FSPEARRLITKLLDPNPV+R GI KITSSSWFKKSVPK+LI K+EQEQTEEN TKSKPPA
Sbjct: 241 FSPEARRLITKLLDPNPVTRAGITKITSSSWFKKSVPKTLIRKDEQEQTEENFTKSKPPA 300

Query: 301 ETLNAFHIISLSEGFDLSPLFEEKKREEKEEMRFATSSPASSVISKLEEVGKAVKFDVKK 360
           ETLNAFHIISLSEGFDLSPLFEEKKREEKEEMRFATSS ASSVISKLEEV KAVKFDVKK
Sbjct: 301 ETLNAFHIISLSEGFDLSPLFEEKKREEKEEMRFATSSTASSVISKLEEVAKAVKFDVKK 360

Query: 361 SESWVRLQGQEKGRKGKLTIAADIFAMTPSFVVVEVKKDNGDTLEYNQFCRKELRPALKD 420
           SE+ VRLQGQE GRKGKLTIAADIFAMTPSFVVVEVKKDNGDTLEYNQFCRKELRPALKD
Sbjct: 361 SETRVRLQGQEMGRKGKLTIAADIFAMTPSFVVVEVKKDNGDTLEYNQFCRKELRPALKD 420

Query: 421 IVWTSPTGNSTPA 434
           IVWTSPTGNSTPA
Sbjct: 421 IVWTSPTGNSTPA 433

BLAST of Lsi11G004020 vs. NCBI nr
Match: XP_022965758.1 (CBL-interacting serine/threonine-protein kinase 6-like [Cucurbita maxima])

HSP 1 Score: 831.6 bits (2147), Expect = 3.0e-237
Identity = 412/433 (95.15%), Postives = 424/433 (97.92%), Query Frame = 0

Query: 1   MADKGRDGGHSSLLDGKYELGRLLGHGTFAKVYHARNLRTGKSVAMKVVGKEKIIKVGMT 60
           MADKGRDGG+SSLLDGKYELGRLLGHGTFAKVYHARNLRTGK+VAMKVVGKEKIIKVGMT
Sbjct: 1   MADKGRDGGYSSLLDGKYELGRLLGHGTFAKVYHARNLRTGKTVAMKVVGKEKIIKVGMT 60

Query: 61  EQIKREISVMKMVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIAKGRLREDVARVY 120
           EQIKREISVMKMVKHPNIVELHEVMASKSKIYFAME VRGGELF+KIAKGRLREDVARVY
Sbjct: 61  EQIKREISVMKMVKHPNIVELHEVMASKSKIYFAMEFVRGGELFTKIAKGRLREDVARVY 120

Query: 121 FQQLISAIDFCHSRGVYHRDLKPENLLLDEDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT 180
           FQQLISAIDFCHSRGVYHRDLKPENLLLDEDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT
Sbjct: 121 FQQLISAIDFCHSRGVYHRDLKPENLLLDEDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT 180

Query: 181 PAYVAPEVIGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFKCPSW 240
           PAYVAPEVIGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMY+KIYRGDFKCP W
Sbjct: 181 PAYVAPEVIGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYKKIYRGDFKCPPW 240

Query: 241 FSPEARRLITKLLDPNPVSRVGIAKITSSSWFKKSVPKSLITKEEQEQTEENLTKSKPPA 300
           FS EARRL+TKLLDPNPV+R+GI KITSSSWFKKSVPK++ITKEEQE+ EEN TKSK PA
Sbjct: 241 FSSEARRLVTKLLDPNPVTRIGITKITSSSWFKKSVPKAIITKEEQERIEENFTKSKQPA 300

Query: 301 ETLNAFHIISLSEGFDLSPLFEEKKREEKEEMRFATSSPASSVISKLEEVGKAVKFDVKK 360
           ETLNAFHIISLSEGFDLSPLFEEKKRE+KEEMRFATSSPASSVISKLEEV KAVKFDVKK
Sbjct: 301 ETLNAFHIISLSEGFDLSPLFEEKKREDKEEMRFATSSPASSVISKLEEVAKAVKFDVKK 360

Query: 361 SESWVRLQGQEKGRKGKLTIAADIFAMTPSFVVVEVKKDNGDTLEYNQFCRKELRPALKD 420
           SESWVRLQGQEKGRKGKL IAADIFA+TPSFVVVEVKKDNGDTLEYNQFCRKELRPALKD
Sbjct: 361 SESWVRLQGQEKGRKGKLAIAADIFALTPSFVVVEVKKDNGDTLEYNQFCRKELRPALKD 420

Query: 421 IVWTSPTGNSTPA 434
           IVWTSPTGNSTPA
Sbjct: 421 IVWTSPTGNSTPA 433

BLAST of Lsi11G004020 vs. NCBI nr
Match: XP_022938287.1 (CBL-interacting serine/threonine-protein kinase 6-like [Cucurbita moschata])

HSP 1 Score: 829.7 bits (2142), Expect = 1.2e-236
Identity = 412/433 (95.15%), Postives = 422/433 (97.46%), Query Frame = 0

Query: 1   MADKGRDGGHSSLLDGKYELGRLLGHGTFAKVYHARNLRTGKSVAMKVVGKEKIIKVGMT 60
           MADKGRDGGHSSLLDGKYELGRLLGHGTFAKVYHARNLRTGK+VAMKVVGKEKII+VGMT
Sbjct: 1   MADKGRDGGHSSLLDGKYELGRLLGHGTFAKVYHARNLRTGKTVAMKVVGKEKIIRVGMT 60

Query: 61  EQIKREISVMKMVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIAKGRLREDVARVY 120
           EQIKREISVMKMVKHPNIVELHEVMASKSKIYFAME VRGGELF+KIAKGRLREDVARVY
Sbjct: 61  EQIKREISVMKMVKHPNIVELHEVMASKSKIYFAMEFVRGGELFTKIAKGRLREDVARVY 120

Query: 121 FQQLISAIDFCHSRGVYHRDLKPENLLLDEDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT 180
           FQQLISAIDFCHSRGVYHRDLKPENLLLDEDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT
Sbjct: 121 FQQLISAIDFCHSRGVYHRDLKPENLLLDEDGNLKVTDFGLTAFSEHLKQDGLLHTTCGT 180

Query: 181 PAYVAPEVIGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFKCPSW 240
           PAYVAPEVIGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMY+KIYRGDFKCP W
Sbjct: 181 PAYVAPEVIGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYKKIYRGDFKCPPW 240

Query: 241 FSPEARRLITKLLDPNPVSRVGIAKITSSSWFKKSVPKSLITKEEQEQTEENLTKSKPPA 300
           FS EARRL+TKLLDPNPV+R+GI KITSSSWFKKSVPKS+ITKEEQE  EEN TKSK PA
Sbjct: 241 FSSEARRLVTKLLDPNPVTRIGITKITSSSWFKKSVPKSIITKEEQEGIEENFTKSKQPA 300

Query: 301 ETLNAFHIISLSEGFDLSPLFEEKKREEKEEMRFATSSPASSVISKLEEVGKAVKFDVKK 360
           ETLNAFHIISLSEGFDLSPLFEEKKRE+KEEMRFATSSPASSVISKLEEV KAVKFDVKK
Sbjct: 301 ETLNAFHIISLSEGFDLSPLFEEKKREDKEEMRFATSSPASSVISKLEEVAKAVKFDVKK 360

Query: 361 SESWVRLQGQEKGRKGKLTIAADIFAMTPSFVVVEVKKDNGDTLEYNQFCRKELRPALKD 420
           SESWVRLQGQEKGRKGKL IAADIFA+TPSFVVVEVKKDNGDTLEYNQFCRKELRPALKD
Sbjct: 361 SESWVRLQGQEKGRKGKLAIAADIFALTPSFVVVEVKKDNGDTLEYNQFCRKELRPALKD 420

Query: 421 IVWTSPTGNSTPA 434
           IVWTSPT NSTPA
Sbjct: 421 IVWTSPTENSTPA 433

BLAST of Lsi11G004020 vs. TAIR 10
Match: AT4G30960.1 (SOS3-interacting protein 3 )

HSP 1 Score: 669.5 bits (1726), Expect = 1.9e-192
Identity = 337/432 (78.01%), Postives = 375/432 (86.81%), Query Frame = 0

Query: 3   DKGRDGGHSS-LLDGKYELGRLLGHGTFAKVYHARNLRTGKSVAMKVVGKEKIIKVGMTE 62
           + G DGG S+ LL G+YELGRLLGHGTFAKVYHARN++TGKSVAMKVVGKEK++KVGM +
Sbjct: 8   ENGSDGGSSTGLLHGRYELGRLLGHGTFAKVYHARNIQTGKSVAMKVVGKEKVVKVGMVD 67

Query: 63  QIKREISVMKMVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIAKGRLREDVARVYF 122
           QIKREISVM+MVKHPNIVELHEVMASKSKIYFAMELVRGGELF+K+AKGRLREDVARVYF
Sbjct: 68  QIKREISVMRMVKHPNIVELHEVMASKSKIYFAMELVRGGELFAKVAKGRLREDVARVYF 127

Query: 123 QQLISAIDFCHSRGVYHRDLKPENLLLDEDGNLKVTDFGLTAFSEHLKQDGLLHTTCGTP 182
           QQLISA+DFCHSRGVYHRDLKPENLLLDE+GNLKVTDFGL+AF+EHLKQDGLLHTTCGTP
Sbjct: 128 QQLISAVDFCHSRGVYHRDLKPENLLLDEEGNLKVTDFGLSAFTEHLKQDGLLHTTCGTP 187

Query: 183 AYVAPEVIGKQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFKCPSWF 242
           AYVAPEVI K+GYDGAKADLWSCGVIL+VLLAGYLPFQDDN+V MYRKIYRGDFKCP W 
Sbjct: 188 AYVAPEVILKKGYDGAKADLWSCGVILFVLLAGYLPFQDDNLVNMYRKIYRGDFKCPGWL 247

Query: 243 SPEARRLITKLLDPNPVSRVGIAKITSSSWFKKSVPKS-------LITKEEQEQTEENLT 302
           S +ARRL+TKLLDPNP +R+ I K+  S WFKK   +S        IT  E E  +  + 
Sbjct: 248 SSDARRLVTKLLDPNPNTRITIEKVMDSPWFKKQATRSRNEPVAATITTTE-EDVDFLVH 307

Query: 303 KSKPPAETLNAFHIISLSEGFDLSPLFEEKKREEKEEMRFATSSPASSVISKLEEVGK-A 362
           KSK   ETLNAFHII+LSEGFDLSPLFEEKK+EEK EMRFATS PASSVIS LEE  +  
Sbjct: 308 KSKEETETLNAFHIIALSEGFDLSPLFEEKKKEEKREMRFATSRPASSVISSLEEAARVG 367

Query: 363 VKFDVKKSESWVRLQGQEKGRKGKLTIAADIFAMTPSFVVVEVKKDNGDTLEYNQFCRKE 422
            KFDV+KSES VR++G++ GRKGKL + A+IFA+ PSFVVVEVKKD+GDTLEYN FC   
Sbjct: 368 NKFDVRKSESRVRIEGKQNGRKGKLAVEAEIFAVAPSFVVVEVKKDHGDTLEYNNFCSTA 427

Query: 423 LRPALKDIVWTS 426
           LRPALKDI WTS
Sbjct: 428 LRPALKDIFWTS 438

BLAST of Lsi11G004020 vs. TAIR 10
Match: AT5G45820.1 (CBL-interacting protein kinase 20 )

HSP 1 Score: 523.1 bits (1346), Expect = 2.2e-148
Identity = 253/414 (61.11%), Postives = 329/414 (79.47%), Query Frame = 0

Query: 17  KYELGRLLGHGTFAKVYHARNLRTGKSVAMKVVGKEKIIKVGMTEQIKREISVMKMVKHP 76
           KYELGRLLG GTFAKVYHARN++TG+SVA+KV+ K+K+ KVG+ +QIKREISVM++V+HP
Sbjct: 11  KYELGRLLGQGTFAKVYHARNIKTGESVAIKVIDKQKVAKVGLIDQIKREISVMRLVRHP 70

Query: 77  NIVELHEVMASKSKIYFAMELVRGGELFSKIAKGRLREDVARVYFQQLISAIDFCHSRGV 136
           ++V LHEVMASK+KIYFAME V+GGELF K++KG+L+E++AR YFQQLI AID+CHSRGV
Sbjct: 71  HVVFLHEVMASKTKIYFAMEYVKGGELFDKVSKGKLKENIARKYFQQLIGAIDYCHSRGV 130

Query: 137 YHRDLKPENLLLDEDGNLKVTDFGLTAFSEHLKQDGLLHTTCGTPAYVAPEVIGKQGYDG 196
           YHRDLKPENLLLDE+G+LK++DFGL+A  E  +QDGLLHTTCGTPAYVAPEVIGK+GYDG
Sbjct: 131 YHRDLKPENLLLDENGDLKISDFGLSALRESKQQDGLLHTTCGTPAYVAPEVIGKKGYDG 190

Query: 197 AKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFKCPSWFSPEARRLITKLLDPN 256
           AKAD+WSCGV+LYVLLAG+LPF + N+V MYRKI +G+FKCP+WF PE ++L++++LDPN
Sbjct: 191 AKADVWSCGVVLYVLLAGFLPFHEQNLVEMYRKITKGEFKCPNWFPPEVKKLLSRILDPN 250

Query: 257 PVSRVGIAKITSSSWFKKSVPKSLITKEEQEQTEENLTKSKPPAETL-----NAFHII-S 316
           P SR+ I KI  +SWF+K   K    K  +    ++L      A ++     NAF +I S
Sbjct: 251 PNSRIKIEKIMENSWFQKGFKKIETPKSPESHQIDSLISDVHAAFSVKPMSYNAFDLISS 310

Query: 317 LSEGFDLSPLFEEKKREEKEEMRFATSSPASSVISKLEEVG-KAVKFDVKKSESWVRLQG 376
           LS+GFDLS LFE   +EE+ E +F T   A  ++SK EE+   + +F++ KS+  V+++ 
Sbjct: 311 LSQGFDLSGLFE---KEERSESKFTTKKDAKEIVSKFEEIATSSERFNLTKSDVGVKMED 370

Query: 377 QEKGRKGKLTIAADIFAMTPSFVVVEVKKDNGDTLEYNQFCRKELRPALKDIVW 424
           + +GRKG L I  +IF +T SF +VE KK  GDT+EY QFC +ELRP+LKDIVW
Sbjct: 371 KREGRKGHLAIDVEIFEVTNSFHMVEFKKSGGDTMEYKQFCDRELRPSLKDIVW 421

BLAST of Lsi11G004020 vs. TAIR 10
Match: AT4G18700.1 (CBL-interacting protein kinase 12 )

HSP 1 Score: 497.3 bits (1279), Expect = 1.3e-140
Identity = 247/440 (56.14%), Postives = 319/440 (72.50%), Query Frame = 0

Query: 12  SLLDGKYELGRLLGHGTFAKVYHARNLRTGKSVAMKVVGKEKIIKVGMTEQIKREISVMK 71
           +L+ G+YE+G+LLGHGTFAKVY ARN++T +SVA+KV+ KEK++K G+   IKREIS+++
Sbjct: 20  ALILGRYEMGKLLGHGTFAKVYLARNVKTNESVAIKVIDKEKVLKGGLIAHIKREISILR 79

Query: 72  MVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIAKGRLREDVARVYFQQLISAIDFC 131
            V+HPNIV+L EVMA+K+KIYF ME VRGGELF+K+AKGRL+E+VAR YFQQLISA+ FC
Sbjct: 80  RVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFNKVAKGRLKEEVARKYFQQLISAVTFC 139

Query: 132 HSRGVYHRDLKPENLLLDEDGNLKVTDFGLTAFSEHLKQDGLLHTTCGTPAYVAPEVIGK 191
           H+RGVYHRDLKPENLLLDE+GNLKV+DFGL+A S+ ++QDGL HT CGTPAYVAPEV+ +
Sbjct: 140 HARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDGLFHTFCGTPAYVAPEVLAR 199

Query: 192 QGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFKCPSWFSPEARRLITK 251
           +GYD AK D+WSCGVIL+VL+AGYLPF D N++AMY+KIYRG+F+CP WFS E  RL++K
Sbjct: 200 KGYDAAKVDIWSCGVILFVLMAGYLPFHDRNVMAMYKKIYRGEFRCPRWFSTELTRLLSK 259

Query: 252 LLDPNPVSRVGIAKITSSSWFKKSV--------PKSLITKEEQEQTEENLTKSK------ 311
           LL+ NP  R    +I  +SWFKK             L    + ++ E +  +S       
Sbjct: 260 LLETNPEKRFTFPEIMENSWFKKGFKHIKFYVEDDKLCNVVDDDELESDSVESDRDSAAS 319

Query: 312 ----------------PPAETLNAFHIISLSEGFDLSPLFEEKKREEKEEMRFATSSPAS 371
                           P   +LNAF IIS S+GFDLS LF+    ++ E  RF + +P S
Sbjct: 320 ESEIEYLEPRRRVGGLPRPASLNAFDIISFSQGFDLSGLFD----DDGEGSRFVSGAPVS 379

Query: 372 SVISKLEEVGKAVKFDVKKSESWVRLQGQEKGRKGKLTIAADIFAMTPSFVVVEVKKDNG 422
            +ISKLEE+ K V F V+K +  V L+G  +G KG LTIAA+IF +TPS VVVEVKK  G
Sbjct: 380 KIISKLEEIAKVVSFTVRKKDCRVSLEGSRQGVKGPLTIAAEIFELTPSLVVVEVKKKGG 439

BLAST of Lsi11G004020 vs. TAIR 10
Match: AT5G07070.1 (CBL-interacting protein kinase 2 )

HSP 1 Score: 494.6 bits (1272), Expect = 8.2e-140
Identity = 247/430 (57.44%), Postives = 329/430 (76.51%), Query Frame = 0

Query: 12  SLLDGKYELGRLLGHGTFAKVYHARNLRTGKSVAMKVVGKEKIIKVGMTEQIKREISVMK 71
           S+L  +YE+GRLLG GTFAKVY  R+  T +SVA+K++ K+K+++VG+++QIKREISVM+
Sbjct: 6   SVLTERYEVGRLLGQGTFAKVYFGRSNHTNESVAIKMIDKDKVMRVGLSQQIKREISVMR 65

Query: 72  MVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIAKGRLREDVARVYFQQLISAIDFC 131
           + KHPN+VEL+EVMA+KS+IYF +E  +GGELF+K+AKG+L+EDVA  YF QLISA+DFC
Sbjct: 66  IAKHPNVVELYEVMATKSRIYFVIEYCKGGELFNKVAKGKLKEDVAWKYFYQLISAVDFC 125

Query: 132 HSRGVYHRDLKPENLLLDEDGNLKVTDFGLTAFSEHLKQDGLLHTTCGTPAYVAPEVIGK 191
           HSRGVYHRD+KPENLLLD++ NLKV+DFGL+A ++  +QDGLLHTTCGTPAYVAPEVI +
Sbjct: 126 HSRGVYHRDIKPENLLLDDNDNLKVSDFGLSALADCKRQDGLLHTTCGTPAYVAPEVINR 185

Query: 192 QGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFKCPSWFSPEARRLITK 251
           +GY+G KAD+WSCGV+L+VLLAGYLPF D N++ MYRKI + DFKCPSWF+PE +RL+ K
Sbjct: 186 KGYEGTKADIWSCGVVLFVLLAGYLPFHDTNLMEMYRKIGKADFKCPSWFAPEVKRLLCK 245

Query: 252 LLDPNPVSRVGIAKITSSSWFKKSV---PKSLITKEEQEQTE-------------ENLTK 311
           +LDPN  +R+ IAKI  SSWF+K +    K +   E+Q+  E             EN   
Sbjct: 246 MLDPNHETRITIAKIKESSWFRKGLHLKQKKMEKMEKQQVREATNPMEAGGSGQNENGEN 305

Query: 312 SKPP-AETLNAFHIISLSEGFDLSPLFEEKKREEKEEMRFATSSPASSVISKLEEVGKAV 371
            +PP   TLNAF II+LS GF L+ LF +    +K E RFA+  PAS +ISKL EV K +
Sbjct: 306 HEPPRLATLNAFDIIALSTGFGLAGLFGDV--YDKRESRFASQKPASEIISKLVEVAKCL 365

Query: 372 KFDVKKSESWV-RLQGQEKGRKGKLTIAADIFAMTPSFVVVEVKKDNGDTLEYNQFCRKE 424
           K  ++K  + + +L+  ++G+ G LT+ A+IF +TP+F +VEVKK NGDT+EY +   ++
Sbjct: 366 KLKIRKQGAGLFKLERVKEGKNGILTMDAEIFQVTPTFHLVEVKKCNGDTMEYQKLVEED 425

BLAST of Lsi11G004020 vs. TAIR 10
Match: AT1G29230.1 (CBL-interacting protein kinase 18 )

HSP 1 Score: 492.7 bits (1267), Expect = 3.1e-139
Identity = 247/440 (56.14%), Postives = 318/440 (72.27%), Query Frame = 0

Query: 11  SSLLDGKYELGRLLGHGTFAKVYHARNLRTGKSVAMKVVGKEKIIKVGMTEQIKREISVM 70
           +++L GKYELG+LLGHGTFAKVY A+N+++G  VA+KV+ KEKI+K G+   IKREIS++
Sbjct: 67  NNILMGKYELGKLLGHGTFAKVYLAQNIKSGDKVAIKVIDKEKIMKSGLVAHIKREISIL 126

Query: 71  KMVKHPNIVELHEVMASKSKIYFAMELVRGGELFSKIAKGRLREDVARVYFQQLISAIDF 130
           + V+HP IV L EVMA+KSKIYF ME V GGELF+ +AKGRL E+ AR YFQQLIS++ F
Sbjct: 127 RRVRHPYIVHLFEVMATKSKIYFVMEYVGGGELFNTVAKGRLPEETARRYFQQLISSVSF 186

Query: 131 CHSRGVYHRDLKPENLLLDEDGNLKVTDFGLTAFSEHLKQDGLLHTTCGTPAYVAPEVIG 190
           CH RGVYHRDLKPENLLLD  GNLKV+DFGL+A +E L+QDGL HT CGTPAY+APEV+ 
Sbjct: 187 CHGRGVYHRDLKPENLLLDNKGNLKVSDFGLSAVAEQLRQDGLCHTFCGTPAYIAPEVLT 246

Query: 191 KQGYDGAKADLWSCGVILYVLLAGYLPFQDDNMVAMYRKIYRGDFKCPSWFSPEARRLIT 250
           ++GYD AKAD+WSCGVIL+VL+AG++PF D N++ MY+KIY+G+F+CP WFS +  RL+T
Sbjct: 247 RKGYDAAKADVWSCGVILFVLMAGHIPFYDKNIMVMYKKIYKGEFRCPRWFSSDLVRLLT 306

Query: 251 KLLDPNPVSRVGIAKITSSSWFKKS-------VPKSLITKEEQEQTEENLTK-------- 310
           +LLD NP +R+ I +I  + WFKK        +    + +E++++ EE  +         
Sbjct: 307 RLLDTNPDTRITIPEIMKNRWFKKGFKHVKFYIEDDKLCREDEDEEEEASSSGRSSTVSE 366

Query: 311 -------------SKPPAETLNAFHIISLSEGFDLSPLFEEKKREEKEEMRFATSSPASS 370
                        S P   +LNAF IIS S GFDLS LFEE   E  E  RF + +P S 
Sbjct: 367 SDAEFDVKRMGIGSMPRPSSLNAFDIISFSSGFDLSGLFEE---EGGEGTRFVSGAPVSK 426

Query: 371 VISKLEEVGKAVKFDVKKSESWVRLQGQEKGRKGKLTIAADIFAMTPSFVVVEVKKDNGD 423
           +ISKLEE+ K V F V+K E  +RL+G  +G KG LTIAA+IF +TPS VVVEVKK  GD
Sbjct: 427 IISKLEEIAKIVSFTVRKKEWSLRLEGCREGAKGPLTIAAEIFELTPSLVVVEVKKKGGD 486

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O655542.6e-19178.01CBL-interacting serine/threonine-protein kinase 6 OS=Arabidopsis thaliana OX=370... [more]
Q9LWM42.1e-15662.65CBL-interacting protein kinase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=CIP... [more]
Q5W7364.2e-14959.82CBL-interacting protein kinase 18 OS=Oryza sativa subsp. japonica OX=39947 GN=CI... [more]
Q6Z9F42.1e-14861.66CBL-interacting protein kinase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=CIP... [more]
Q9FJ543.0e-14761.11CBL-interacting serine/threonine-protein kinase 20 OS=Arabidopsis thaliana OX=37... [more]
Match NameE-valueIdentityDescription
A0A0A0LL733.7e-24197.23Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_2... [more]
A0A5D3BFR33.0e-23896.54Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194... [more]
A0A6J1HPX11.5e-23795.15Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1FDM55.6e-23795.15Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1KAP01.9e-23293.76Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
Match NameE-valueIdentityDescription
XP_038890150.11.8e-24297.69CBL-interacting serine/threonine-protein kinase 6-like [Benincasa hispida][more]
XP_004139258.17.7e-24197.23CBL-interacting serine/threonine-protein kinase 6 [Cucumis sativus] >KGN60761.1 ... [more]
KAA0063563.16.1e-23896.54CBL-interacting serine/threonine-protein kinase 6-like [Cucumis melo var. makuwa... [more]
XP_022965758.13.0e-23795.15CBL-interacting serine/threonine-protein kinase 6-like [Cucurbita maxima][more]
XP_022938287.11.2e-23695.15CBL-interacting serine/threonine-protein kinase 6-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT4G30960.11.9e-19278.01SOS3-interacting protein 3 [more]
AT5G45820.12.2e-14861.11CBL-interacting protein kinase 20 [more]
AT4G18700.11.3e-14056.14CBL-interacting protein kinase 12 [more]
AT5G07070.18.2e-14057.44CBL-interacting protein kinase 2 [more]
AT1G29230.13.1e-13956.14CBL-interacting protein kinase 18 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 18..272
e-value: 6.3E-103
score: 357.9
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 18..272
e-value: 5.9E-73
score: 245.5
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 18..272
score: 51.000431
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 15..99
e-value: 4.7E-28
score: 99.1
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 100..298
e-value: 9.3E-59
score: 200.1
NoneNo IPR availableGENE3D3.30.310.80Kinase associated domain 1, KA1coord: 299..424
e-value: 3.8E-46
score: 158.2
NoneNo IPR availablePIRSRPIRSR000620-2PIRSR000620-2coord: 17..211
e-value: 2.0E-11
score: 41.3
NoneNo IPR availablePIRSRPIRSR037921-2PIRSR037921-2coord: 22..221
e-value: 9.2E-9
score: 32.8
NoneNo IPR availablePANTHERPTHR43895FAMILY NOT NAMEDcoord: 10..430
NoneNo IPR availablePANTHERPTHR43895:SF53NON-SPECIFIC SERINE/THREONINE PROTEIN KINASEcoord: 10..430
NoneNo IPR availableCDDcd12195CIPK_Ccoord: 304..419
e-value: 3.70918E-46
score: 153.497
NoneNo IPR availableCDDcd14663STKc_SnRK3coord: 17..271
e-value: 3.94598E-172
score: 480.364
IPR004041NAF domainPFAMPF03822NAFcoord: 301..360
e-value: 1.5E-22
score: 79.5
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 136..148
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 24..51
IPR018451NAF/FISL domainPROSITEPS50816NAFcoord: 298..322
score: 12.275091
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 15..292

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi11G004020.1Lsi11G004020.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
biological_process GO:0007165 signal transduction
molecular_function GO:0005524 ATP binding
molecular_function GO:0106310 protein serine kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004712 protein serine/threonine/tyrosine kinase activity
molecular_function GO:0004672 protein kinase activity