Lsi11G001550 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi11G001550
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	RING-type domain-containing protein
Location	chr11: 1693093 .. 1694045 (-)
RNA-Seq Expression	Lsi11G001550
Synteny	Lsi11G001550

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGGAGGATGCGAACTACTATGCTAAAACCCTACTTGCCAAATTGTCTTATCGGTTCTGTTTCTCTGGTCTCCCTGTTGGAGGTGTAACCCCTATTTGCATCTTTTGGTAGCTAATAGTGGATAAGATCAAAGCTAGACAACCAGAATGGAAGATATTCGAATGTTGAAACGTTAGAATGACTTTAGTCCAATCGGGTATCTTAAATCTTCCAAACCATTTATTTTTTCTAATTGGTGTGCCTTCACAATTTTATTTATTTATTTATTTTTTTGTAGTTACATCAAGTAGTTGCAAATCTTACAGTCTAAATGTTCTGTTTCTGTTTGAGAAATTAAAGGGAAGAAACTGTTCATGTGGATGTTGATGTTAATAGTTCAAACTTATGGGCTATGTTGACAAATATTCTCTCTTTAATAAAGATCATGGATGCAATCGGAATTTGCAAAAAATCACTTATATCAATTGCTTATTATTAGTCATAAATCATATTGAATTTGTAAAGATGATGATCAAGTCTGTTATTCTAGCTTCATATTGTTTTTTAGTCTGGTTAGTTAAAACTTTTTCCAACTTATGGCATGAAACATTCTCTTGCAATTATCACCAAACACGTATGTGCCTTTTTTGCCCCTGATGTGAAATTATAGAAGGAGCCAAAGGCATGGTGTTGAAAGTCTTGTGTTTGGTATTTGTAGGAGTGTGTAATCATGCATTTCATTTCCACTGTATCAGTAGATGGTTGAAGACACGTCAAGTTTGCCCATTGGGTATGCGTTTCAAGTTCTTCCCCTACTCTAGTCTTATCCCACATCTTTATATGAACTTTGAAAATTTAAAGGGAGCTTTGCCATTTTTACAGATAATAGCGAGTGGGAATTTCAGAAGTACGGTCACTAGAACTTTCATGCTGTTGAAGGATATGATCCTTAAAATTCTCATGGGGCTATAA

mRNA sequence

ATGTGGAGGATGCGAACTACTATGCTAAAACCCTACTTGCCAAATTGTCTTATCGGTTCTGTTTCTCTGGTCTCCCTGTTGGAGGTAATGACTTTAGTCCAATCGGTTACATCAAGTAGTTGCAAATCTTACAGTCTAAATGTTCTGTTTCTGTTTGAGAAATTAAAGGGAAGAAACTGTTCATGTGGATTCATAAATCATATTGAATTTGTAAAGATGATGATCAAGTCTGTTATTCTAGCTTCATATTGTTTTTTAGTCTGGTTAGTTAAAACTTTTTCCAACTTATGGCATGAAACATTCTCTTGCAATTATCACCAAACACGAGTGTGTAATCATGCATTTCATTTCCACTGTATCAGTAGATGGTTGAAGACACGTCAAGTTTGCCCATTGGGTATGCGTTTCAAGTTCTTCCCCTACTCTAGTCTTATCCCACATCTTTATATGAACTTTGAAAATTTAAAGGGAGCTTTGCCATTTTTACAGATAATAGCGAGTGGGAATTTCAGAAGTACGGTCACTAGAACTTTCATGCTGTTGAAGGATATGATCCTTAAAATTCTCATGGGGCTATAA

Coding sequence (CDS)

ATGTGGAGGATGCGAACTACTATGCTAAAACCCTACTTGCCAAATTGTCTTATCGGTTCTGTTTCTCTGGTCTCCCTGTTGGAGGTAATGACTTTAGTCCAATCGGTTACATCAAGTAGTTGCAAATCTTACAGTCTAAATGTTCTGTTTCTGTTTGAGAAATTAAAGGGAAGAAACTGTTCATGTGGATTCATAAATCATATTGAATTTGTAAAGATGATGATCAAGTCTGTTATTCTAGCTTCATATTGTTTTTTAGTCTGGTTAGTTAAAACTTTTTCCAACTTATGGCATGAAACATTCTCTTGCAATTATCACCAAACACGAGTGTGTAATCATGCATTTCATTTCCACTGTATCAGTAGATGGTTGAAGACACGTCAAGTTTGCCCATTGGGTATGCGTTTCAAGTTCTTCCCCTACTCTAGTCTTATCCCACATCTTTATATGAACTTTGAAAATTTAAAGGGAGCTTTGCCATTTTTACAGATAATAGCGAGTGGGAATTTCAGAAGTACGGTCACTAGAACTTTCATGCTGTTGAAGGATATGATCCTTAAAATTCTCATGGGGCTATAA

Protein sequence

MWRMRTTMLKPYLPNCLIGSVSLVSLLEVMTLVQSVTSSSCKSYSLNVLFLFEKLKGRNCSCGFINHIEFVKMMIKSVILASYCFLVWLVKTFSNLWHETFSCNYHQTRVCNHAFHFHCISRWLKTRQVCPLGMRFKFFPYSSLIPHLYMNFENLKGALPFLQIIASGNFRSTVTRTFMLLKDMILKILMGL

Homology

[Close]

BLAST of Lsi11G001550 vs. ExPASy Swiss-Prot
Match: Q9W5E1 (RING-box protein 1A OS=Drosophila melanogaster OX=7227 GN=Roc1a PE=1 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 5.5e-08
Identity = 24/26 (92.31%), Postives = 24/26 (92.31%), Query Frame = 0


Query: 110 VCNHAFHFHCISRWLKTRQVCPLGMR 136
           VCNHAFHFHCISRWLKTRQVCPL  R
Sbjct: 74  VCNHAFHFHCISRWLKTRQVCPLDNR 99

[Close]

BLAST of Lsi11G001550 vs. ExPASy Swiss-Prot
Match: P62877 (E3 ubiquitin-protein ligase RBX1 OS=Homo sapiens OX=9606 GN=RBX1 PE=1 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 5.5e-08
Identity = 24/26 (92.31%), Postives = 24/26 (92.31%), Query Frame = 0


Query: 110 VCNHAFHFHCISRWLKTRQVCPLGMR 136
           VCNHAFHFHCISRWLKTRQVCPL  R
Sbjct: 74  VCNHAFHFHCISRWLKTRQVCPLDNR 99

[Close]

BLAST of Lsi11G001550 vs. ExPASy Swiss-Prot
Match: P62878 (E3 ubiquitin-protein ligase RBX1 OS=Mus musculus OX=10090 GN=Rbx1 PE=1 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 5.5e-08
Identity = 24/26 (92.31%), Postives = 24/26 (92.31%), Query Frame = 0


Query: 110 VCNHAFHFHCISRWLKTRQVCPLGMR 136
           VCNHAFHFHCISRWLKTRQVCPL  R
Sbjct: 74  VCNHAFHFHCISRWLKTRQVCPLDNR 99

[Close]

BLAST of Lsi11G001550 vs. ExPASy Swiss-Prot
Match: Q8QG64 (RING-box protein 1 OS=Salmo salar OX=8030 GN=rbx1 PE=2 SV=2)

HSP 1 Score: 59.3 bits (142), Expect = 5.5e-08
Identity = 24/26 (92.31%), Postives = 24/26 (92.31%), Query Frame = 0


Query: 110 VCNHAFHFHCISRWLKTRQVCPLGMR 136
           VCNHAFHFHCISRWLKTRQVCPL  R
Sbjct: 74  VCNHAFHFHCISRWLKTRQVCPLDNR 99

[Close]

BLAST of Lsi11G001550 vs. ExPASy Swiss-Prot
Match: Q940X7 (RING-box protein 1a OS=Arabidopsis thaliana OX=3702 GN=RBX1A PE=1 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 9.4e-08
Identity = 23/23 (100.00%), Postives = 23/23 (100.00%), Query Frame = 0


Query: 110 VCNHAFHFHCISRWLKTRQVCPL 133
           VCNHAFHFHCISRWLKTRQVCPL
Sbjct: 84  VCNHAFHFHCISRWLKTRQVCPL 106

[Close]

BLAST of Lsi11G001550 vs. ExPASy TrEMBL
Match: A0A4V3WR40 (RING-type domain-containing protein OS=Camellia sinensis var. sinensis OX=542762 GN=TEA_005098 PE=4 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 1.6e-10
Identity = 46/87 (52.87%), Postives = 57/87 (65.52%), Query Frame = 0


Query: 110 VCNHAFHFHCISRWLKTRQVCPLGMRFKFFP--YSSLIPH----LYMNFENLKGALPFLQ 169
           VCNHAFHFHCISRWLKTRQVCPLG   KF+   Y +L  H    L ++F    G+L F+Q
Sbjct: 92  VCNHAFHFHCISRWLKTRQVCPLG---KFYAPYYYALDSHKCDCLLISFFFRCGSL-FVQ 151

Query: 170 IIASGNFRSTVTRTFMLLKDMILKILM 191
           I ASG+FRS  TR F+ +     + L+
Sbjct: 152 ITASGSFRSMATRCFVQVNSWSSRFLL 174

[Close]

BLAST of Lsi11G001550 vs. ExPASy TrEMBL
Match: A0A4S4F121 (Uncharacterized protein OS=Camellia sinensis var. sinensis OX=542762 GN=TEA_005095 PE=4 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 8.0e-10
Identity = 39/67 (58.21%), Postives = 48/67 (71.64%), Query Frame = 0


Query: 110 VCNHAFHFHCISRWLKTRQVCPLGMRFKFFPYSSLIPH----LYMNFENLKGALPFLQII 169
           VCNHAFHFHCISRWLKTRQVCPLG  +  + Y +L  H    L ++F    G+L F+QI 
Sbjct: 92  VCNHAFHFHCISRWLKTRQVCPLGKFYAPYYYYALNSHQCDCLLISFLFRCGSL-FVQIT 151

Query: 170 ASGNFRS 173
           ASG+FR+
Sbjct: 152 ASGSFRN 157

[Close]

BLAST of Lsi11G001550 vs. ExPASy TrEMBL
Match: A0A7J7CHG7 (RING-type domain-containing protein OS=Tripterygium wilfordii OX=458696 GN=HS088_TW17G01041 PE=4 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 7.4e-08
Identity = 38/68 (55.88%), Postives = 39/68 (57.35%), Query Frame = 0


Query: 110 VCNHAFHFHCISRWLKTRQVCPLGMRFKFFPYSSLIPHLYMNFENLKGALPFLQIIASGN 169
           VCNHAFHFHCISRWLKTRQVCPLG            P L             LQIIASGN
Sbjct: 82  VCNHAFHFHCISRWLKTRQVCPLG-----------DPSL-------------LQIIASGN 125

Query: 170 FRSTVTRT 178
            RS  T+T
Sbjct: 142 SRSMATKT 125

[Close]

BLAST of Lsi11G001550 vs. ExPASy TrEMBL
Match: A0A6P5FVJ8 (uncharacterized protein LOC109718511 OS=Ananas comosus OX=4615 GN=LOC109718511 PE=4 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 3.7e-07
Identity = 26/30 (86.67%), Postives = 28/30 (93.33%), Query Frame = 0


Query: 107 QTRVCNHAFHFHCISRWLKTRQVCPLGMRF 137
           QTRVCNHAFHFHCIS+WLK RQVCPLG+ F
Sbjct: 103 QTRVCNHAFHFHCISQWLKNRQVCPLGIYF 132

[Close]

BLAST of Lsi11G001550 vs. ExPASy TrEMBL
Match: A0A0S3SHB7 (RING-type domain-containing protein OS=Vigna angularis var. angularis OX=157739 GN=Vigan.07G085400 PE=4 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 4.8e-07
Identity = 27/29 (93.10%), Postives = 27/29 (93.10%), Query Frame = 0


Query: 110 VCNHAFHFHCISRWLKTRQVCPLGMRFKF 139
           VCNHAFHFHCISRWLKTRQVCPLGM  KF
Sbjct: 84  VCNHAFHFHCISRWLKTRQVCPLGMWDKF 112

[Close]

BLAST of Lsi11G001550 vs. NCBI nr
Match: THG22707.1 (hypothetical protein TEA_005098 [Camellia sinensis var. sinensis])

HSP 1 Score: 76.3 bits (186), Expect = 3.3e-10
Identity = 46/87 (52.87%), Postives = 57/87 (65.52%), Query Frame = 0


Query: 110 VCNHAFHFHCISRWLKTRQVCPLGMRFKFFP--YSSLIPH----LYMNFENLKGALPFLQ 169
           VCNHAFHFHCISRWLKTRQVCPLG   KF+   Y +L  H    L ++F    G+L F+Q
Sbjct: 92  VCNHAFHFHCISRWLKTRQVCPLG---KFYAPYYYALDSHKCDCLLISFFFRCGSL-FVQ 151

Query: 170 IIASGNFRSTVTRTFMLLKDMILKILM 191
           I ASG+FRS  TR F+ +     + L+
Sbjct: 152 ITASGSFRSMATRCFVQVNSWSSRFLL 174

[Close]

BLAST of Lsi11G001550 vs. NCBI nr
Match: THG22704.1 (hypothetical protein TEA_005095 [Camellia sinensis var. sinensis])

HSP 1 Score: 73.9 bits (180), Expect = 1.6e-09
Identity = 39/67 (58.21%), Postives = 48/67 (71.64%), Query Frame = 0


Query: 110 VCNHAFHFHCISRWLKTRQVCPLGMRFKFFPYSSLIPH----LYMNFENLKGALPFLQII 169
           VCNHAFHFHCISRWLKTRQVCPLG  +  + Y +L  H    L ++F    G+L F+QI 
Sbjct: 92  VCNHAFHFHCISRWLKTRQVCPLGKFYAPYYYYALNSHQCDCLLISFLFRCGSL-FVQIT 151

Query: 170 ASGNFRS 173
           ASG+FR+
Sbjct: 152 ASGSFRN 157

[Close]

BLAST of Lsi11G001550 vs. NCBI nr
Match: KAF5733497.1 (hypothetical protein HS088_TW17G01041 [Tripterygium wilfordii])

HSP 1 Score: 67.4 bits (163), Expect = 1.5e-07
Identity = 38/68 (55.88%), Postives = 39/68 (57.35%), Query Frame = 0


Query: 110 VCNHAFHFHCISRWLKTRQVCPLGMRFKFFPYSSLIPHLYMNFENLKGALPFLQIIASGN 169
           VCNHAFHFHCISRWLKTRQVCPLG            P L             LQIIASGN
Sbjct: 82  VCNHAFHFHCISRWLKTRQVCPLG-----------DPSL-------------LQIIASGN 125

Query: 170 FRSTVTRT 178
            RS  T+T
Sbjct: 142 SRSMATKT 125

[Close]

BLAST of Lsi11G001550 vs. NCBI nr
Match: CAA2967018.1 (RING-box 1a [Olea europaea subsp. europaea])

HSP 1 Score: 67.0 bits (162), Expect = 2.0e-07
Identity = 28/36 (77.78%), Postives = 30/36 (83.33%), Query Frame = 0


Query: 110 VCNHAFHFHCISRWLKTRQVCPLGMRFKFFPYSSLI 146
           VCNHAFHFHCISRWLKTRQVCPLG     FP+S +I
Sbjct: 91  VCNHAFHFHCISRWLKTRQVCPLGKLLYCFPFSIII 126

[Close]

BLAST of Lsi11G001550 vs. NCBI nr
Match: CAA2960678.1 (RING-box 1a [Olea europaea subsp. europaea])

HSP 1 Score: 66.2 bits (160), Expect = 3.4e-07
Identity = 28/35 (80.00%), Postives = 29/35 (82.86%), Query Frame = 0


Query: 110 VCNHAFHFHCISRWLKTRQVCPLGMRFKFFPYSSL 145
           VCNHAFHFHCISRWLKTRQVCPLG     FP+S L
Sbjct: 91  VCNHAFHFHCISRWLKTRQVCPLGKLLYSFPFSLL 125

[Close]

BLAST of Lsi11G001550 vs. TAIR 10
Match: AT5G20570.2 (RING-box 1 )

HSP 1 Score: 59.3 bits (142), Expect = 3.9e-09
Identity = 24/27 (88.89%), Postives = 24/27 (88.89%), Query Frame = 0


Query: 106 HQTRVCNHAFHFHCISRWLKTRQVCPL 133
           H   VCNHAFHFHCISRWLKTRQVCPL
Sbjct: 104 HLEGVCNHAFHFHCISRWLKTRQVCPL 130

[Close]

BLAST of Lsi11G001550 vs. TAIR 10
Match: AT3G42830.1 (RING/U-box superfamily protein )

HSP 1 Score: 58.5 bits (140), Expect = 6.7e-09
Identity = 23/23 (100.00%), Postives = 23/23 (100.00%), Query Frame = 0


Query: 110 VCNHAFHFHCISRWLKTRQVCPL 133
           VCNHAFHFHCISRWLKTRQVCPL
Sbjct: 81  VCNHAFHFHCISRWLKTRQVCPL 103

[Close]

BLAST of Lsi11G001550 vs. TAIR 10
Match: AT5G20570.1 (RING-box 1 )

HSP 1 Score: 58.5 bits (140), Expect = 6.7e-09
Identity = 23/23 (100.00%), Postives = 23/23 (100.00%), Query Frame = 0


Query: 110 VCNHAFHFHCISRWLKTRQVCPL 133
           VCNHAFHFHCISRWLKTRQVCPL
Sbjct: 84  VCNHAFHFHCISRWLKTRQVCPL 106

[Close]

BLAST of Lsi11G001550 vs. TAIR 10
Match: AT5G20570.3 (RING-box 1 )

HSP 1 Score: 58.5 bits (140), Expect = 6.7e-09
Identity = 23/23 (100.00%), Postives = 23/23 (100.00%), Query Frame = 0


Query: 110 VCNHAFHFHCISRWLKTRQVCPL 133
           VCNHAFHFHCISRWLKTRQVCPL
Sbjct: 102 VCNHAFHFHCISRWLKTRQVCPL 124

The following BLAST results are available for this feature:

BLAST of Lsi11G001550 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9W5E1	5.5e-08	92.31	RING-box protein 1A OS=Drosophila melanogaster OX=7227 GN=Roc1a PE=1 SV=1	[more]
P62877	5.5e-08	92.31	E3 ubiquitin-protein ligase RBX1 OS=Homo sapiens OX=9606 GN=RBX1 PE=1 SV=1	[more]
P62878	5.5e-08	92.31	E3 ubiquitin-protein ligase RBX1 OS=Mus musculus OX=10090 GN=Rbx1 PE=1 SV=1	[more]
Q8QG64	5.5e-08	92.31	RING-box protein 1 OS=Salmo salar OX=8030 GN=rbx1 PE=2 SV=2	[more]
Q940X7	9.4e-08	100.00	RING-box protein 1a OS=Arabidopsis thaliana OX=3702 GN=RBX1A PE=1 SV=1	[more]

BLAST of Lsi11G001550 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A4V3WR40	1.6e-10	52.87	RING-type domain-containing protein OS=Camellia sinensis var. sinensis OX=542762...	[more]
A0A4S4F121	8.0e-10	58.21	Uncharacterized protein OS=Camellia sinensis var. sinensis OX=542762 GN=TEA_0050...	[more]
A0A7J7CHG7	7.4e-08	55.88	RING-type domain-containing protein OS=Tripterygium wilfordii OX=458696 GN=HS088...	[more]
A0A6P5FVJ8	3.7e-07	86.67	uncharacterized protein LOC109718511 OS=Ananas comosus OX=4615 GN=LOC109718511 P...	[more]
A0A0S3SHB7	4.8e-07	93.10	RING-type domain-containing protein OS=Vigna angularis var. angularis OX=157739 ...	[more]

BLAST of Lsi11G001550 vs. NCBI nr


Match Name	E-value	Identity	Description
THG22707.1	3.3e-10	52.87	hypothetical protein TEA_005098 [Camellia sinensis var. sinensis]	[more]
THG22704.1	1.6e-09	58.21	hypothetical protein TEA_005095 [Camellia sinensis var. sinensis]	[more]
KAF5733497.1	1.5e-07	55.88	hypothetical protein HS088_TW17G01041 [Tripterygium wilfordii]	[more]
CAA2967018.1	2.0e-07	77.78	RING-box 1a [Olea europaea subsp. europaea]	[more]
CAA2960678.1	3.4e-07	80.00	RING-box 1a [Olea europaea subsp. europaea]	[more]

BLAST of Lsi11G001550 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G20570.2	3.9e-09	88.89	RING-box 1	[more]
AT3G42830.1	6.7e-09	100.00	RING/U-box superfamily protein	[more]
AT5G20570.1	6.7e-09	100.00	RING-box 1	[more]
AT5G20570.3	6.7e-09	100.00	RING-box 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR024766	Zinc finger, RING-H2-type	PFAM	PF12678	zf-rbx1	coord: 109..132 e-value: 2.1E-10 score: 40.7
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 81..139 e-value: 2.0E-8 score: 36.1
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 106..134

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi11G001550.1	Lsi11G001550.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0031145	anaphase-promoting complex-dependent catabolic process
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005680	anaphase-promoting complex
molecular_function	GO:0097602	cullin family protein binding
molecular_function	GO:0061630	ubiquitin protein ligase activity
molecular_function	GO:0008270	zinc ion binding