Lsi10G012850 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi10G012850
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionisovaleryl-CoA dehydrogenase, mitochondrial
Locationchr10: 17092665 .. 17097937 (-)
RNA-Seq ExpressionLsi10G012850
SyntenyLsi10G012850
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTCAAATTGTTCCCCATCTCTCTTCCCTTTGTTCTTGTTGGAGCCAATTTCTCTGTAAACAAGATGCAGAAGCTCTTCGCTGCAAGGACTGCCTGTGCTTCCATTCTTAGGAGACAGAGACATTCTGCTTCTTTCTCTTCTGCTCTGCTTTTTGATGATACTCAACTACAGGTCTCGATTTATTGTTTGCTAATGACGATGAATGGGACTTGTTGATTTTGTTGTCGATGGCTTTCTATTTTGGATCTTTATTGGTTAACTTAGATTTGTTAGTATGTGATGCGAATTTATGACCATGAAGCTCGAATTGAGAATTGTTTGTTTTGTTTGTATGCATGGTTTTTTTAAGATAATTTTTGGTTAAAATGAATTCAACCCAGTTGGTATCTGTAGGATGATGAAGGTTAAAGAATGGTATAGGTTTTTGTTTTTAATTTTCTAAGATAATTGATTGATTATTGCAGTTCAAAGAAAGTGTTAAACAATTTGCTCAAGATAACATTGCTCCCCATGCCGCAAAAATTGATCAAACAAACTCTTTTCCTCAGGTCAGTTGGATGTCCTTCTCTTCTGCCACCCCATATTTAATGGGTTACTTTATGATTTGACTAAAATGTACGTTATTGTAGGATGTTAACTTATGGAAGCTCATGGGGGACTTCAATCTCCATGGAATTACTGCACCAGGTAGAGGGTTAGAAATCATTTTTTGGATGTTTCCCTCATTCCCTGTTATTTGCAGCGGTTCAATACCTTAAAAATTATATTATATTCTCTTTCATTTTTCCACTTCTAGTTTTTTTCCATGAACTTCTATTTAATTGTGCTCTTAATGTACATTGTATCCTCATAAAGTTAAATTTAATTGAGATATTTGATACATAGTAAGTGCCTCCTTAAATTAGATGAACATTGAGATGATTTTATATATTTTGCTAGTGCATTGTGTTTCTCATTCTTTAAAGATATTTTGAAACTAACTATTGGGTGTCTTGTAGAGGAATATGGAGGACTTGGTCTGGGTTACTTGTATCACTGTATGGCAATGGAAGAAATAAGTCGCGCTTCTGGGTCTGTTGCCCTCTCCTTTGGTGCACATTCAAACCTGTGCATCAATCAGCTGGTAAAGTGAAGCATGATTATACACTTTGTTATTCATATCATATATATTGTCTTAGACAATATGAACTACATGTGTTATCTTAATCATATATGTTTTTTTTTTCCAGGTGAGGAATGGAAACCCAGCTCAAAAAGAAAAGTACTTGCCTAAGGTTTTATGCCTTTCTGCCTACACTCTTTGTTCCCCATTCCCTTAATAATGTTCATGTTATAATAATGATTATCTAACTGCACATAACAATGTCATGCTCATTTCATTTTCTTTGATCCTAGCCATATGGCATTGTCATGCCAGAAAATTTTTGTTTTTTCATTTTCAATATAACTGTTGAAGTAAAATTTTTCATTCCTTCACGATTTATTTTTTCTGAAGAATAAAATTAGTCTTTCACGCATTATTATCTCTATTTCCTTCTCATTGCTCCTTTCTTTTCCATTAAAGTAATTCTTTTTGGCTACTAACTTAAATATATCTTTTTTAATAAGCTTATTAGAGGGGAGCATGTGGGAGCTCTTGCAATGAGTGAACCCAATGGTAACTTCAGTGAACTTTTGTTTTTCACGTATGCATATAAAGGGAGATCTTTATAAGGACTAAGAATATTTTCATCCTTGAATAATGCACTTCCTACTTTCAGCTGGCTCGGATGTTGTGAGCATGAAATGCAAAGCTGACCGGGTGGATGGTGGATATGTCATTAATGGAAACAAGATGTGGTGTACTAATGGTCCTACCGCTCAAACATTGGTATTTTTTGTTGCTAATTTTCTAATTGTGTTAAAAAGAATTTCCTCTTTATTTCTGGTTAATGTGTGTGTCACCTAAGAAAAGAGGTATTTCTAGATAATGTATATTATCTGGAGTTTTTTCATTGTAGCTATTTGGTGAGAACTTTTCTTTTTCCGGATTCAGGGGTCTATCATGAACAACCTTGCACTTGTTATATGCTTGTTCAAAACAGTGTCTTGAACTTAATATTTCGTTGTTATAGTATGTTAGTATGAACCACCTATATTTTCAGTGAAATGGGGACCTTTCTCTACAATTACCCAGGCAAAAGCTGATTTTTTTGTGTAAACAACAAAGCAACAAACCAAAGGGCAGACTATCCCGTGGTTTTATTTTATGTTATTTTATGTCTTTTCATGTAATGTGGGAGGGAACAAACAGGTTGAGGGAGTAACATTTTTTAGTAACAGGTTAGATTTTGCAGTTTGCTTCCACCTTAAAATCTTAGAAGCATGTAGGTTGAACAATTCATGGTTGCTGAAACGTTTTATTCTTGCATATTTCATTGACTCTGAGCCAATTTACACAATTGAAACAGGTTGTTTATGCAAAAACGGATGCAAATGCTGGCTCAAAGGGAATTACAGCATTTATCATCGAGAAAGGAATGCCCGGGTACTTAAAAATCTGTTGGTCTGGTTCATGATATGTTTAATTTGCAAGTAACAGAATTTTCTGTTCTGTTTCTCTAGGATATTCTAGCGTAGCACTATACCATATTTAAAAATTGTAATATTAAAATAACCCCCAGCTCCTGATTCCAAGTTCATTTAGAAACTTCCAGCGTTCCTTATCCTTGATTCTTATATCGGCTGCTTCTATGTTTCTCAGATTCAGTACTGCCCAGAAATTAGACAAACTTGGCATGCGAGGGAGTGATACGTGAGTCTACTGTGCTTGGAATTTCAAGTTTCTAAAATAAGCACATGCCATCTTCTTTCCTCTTCCACTCATGTTCGCAATCGTTTGTACAGATGTGAGCTTGTCTTTGAAAATTGCTTTGTTCCTGAAGAAAATATACTTGGACAAGAAGGAAAAGGTATACTGCTGCTGTAATATTGTTATTCATTCATTCATCTAGTAAATACTTATATTTTTTAATATTTTGTCTCAGTAAAATCATTTCAATGGGGTTGGATGTGTTATTGATGGGGTATTAAATAGCGGCAGATTTCAACGCAGGTTTCTAATTTATGGCCATAGCCTAATTGATTAATCTATTTGGTATTCTCAGTTAAAATATCAAGCCATTCCACAAAAGAAAACAAAATATTCTGTGATCTTATCGTTAGGAGTCTTTGAACCAAGATATTCATATTTGCTGTGTAGGTAAGGGTAGTTTTCTGACCAAATGTTGTGACAGCTAAGAGTATCTAAAATGCTGACATTTTAGGCTCTAATGGCATCTCATAGGAGAATTTTTCAACCGTAACGGCCCTTTCATGTCTGGTTTGGGTTTAATGTAAGGGCCTTACTTTGGCATTGTCAACCCACCATGGGTTGGCCCCTTGGTCTAGTGGCCATAAAAAACCCATATAAATAGTAAACGACTTACAAGAAATGCGTTCAAATCATGTTCTTACCTACTTAAAATTTAATATCCCACTAGTTATTTTGGTAACCAAAATAGTAGGGTGAGACGATTGTCCCGTGAGAATGGTCAAGCAAGCCGGCCCAGACACTCAATAAAATGGAAAAAATAATAATAAGAAAAAAATAAAATAAGAAAAATGCTGCTGTCTGTCTTGAAAATTCTTGCTATAGTCGAGTTCAACAACAAGTAGAGGTTTAGAATTTGAACCTTTGGCCTCTTGGCTAAGTATATTTACATTAAGAAGTTGAGCTATGTTCATGTTGGCGACAGTTGCTATGATAAATATGAATGTGAATAGTCACTTCGCTTCACTACCTCGTCTTGTTTCCAGTCAGATTGGAAGAGAACCAAACTATTATCATCTTCATATTGATCAATTTTTTTTTCAGGAGTTTATGTTATGATGTCTGGGTTAGATTTGGAAAGGCTTGTTTTGGCTGCTGGTCCCCTAGGTCTTATGCAAGCATGCCTTGATGTTGTTCTTCCTTACGTTAAACAAAGAGAGCAGTTTGGTCGTCCAATTGGGGAATTCCAGTTTATACAGGTTCTATCACTGTGACTATCTTACCATATACATCGCTCATAGTTCCTCTAAAATGACTCAACTAATCTGCAATTCCAGGGAAAACTTGCTGATATGTATACGTCTTTGCAATCATCAAGGTGAGAGATTCTGGAGTAATCATGTTTTTTTCTTTTTCCTTTTCCTTTTTCTTGTCATGTTAACTCTCTCTCTCTCTCTTTCTCCAGGGCCTATGTTTATTCTGTTGCTAGGGACTGTGACAGTGGAAAAGTTGATCCCAAGGTTTGATGTAGATCTTAAGCTTTTTTTGTTGCTTCTTGAATCTTCAATGAATATTTGTACATGTTCATACTATCTGTATGCTTTTGACCCATTTTTTCTTTTTCCATGGCCATGAGATTATTTGGCCAAGGGAACAAAAATGTGGTTCGATTCCGCAACATAAAATCCTTCAAGTTTTCACCTCGTGCGATTTTGTTTCCAGGATTGTGCTGGAGTAATATTATGTGCAGCTGAAAGAGCAACACAAGTTGCTCTCCAGGTAAAATCTGTGTTCACGAAAACATTTCCTTTTTATCCAATAGGATCATATTACTTCTATGTTCCATGCCATAGCAGGACCTTGTGAAAAAAGTAACACCAACTCCAAGTTGATGGGGAAATCCTTCATGTTCTATCTTGCAGGCCATACAATGCTTAGGAGGCAACGGATATGTAAACGAGTACGTAACCGGTCGACTACTTCGAGATGCAAAACTATATGAAATAGGTGCAGGTACTAGCGAAGTGAGAAGGATGATCATCGGTCGCTCACTGTTCAAAGAACAATAGGAGACAACTTTTACTCTCAATTGGATTGCTTCAGAGGAAACATCCCACTACCAAAGTCAATAATACTCAATACTCATCAACCCCCAGAGCAGGTTCTTAAGAAGCTAAGGTGATTGATGATTGAGGTCTTTATCTACGATGTGTAATTTTCAGGGAACATTGTTCAAATTTGTCATCATAATAATTGTTGCCTTTCATTGACATTGATAGAAAATAAAATGAAAGAAAGTCAATCAAATCAGGGAAAACGTGGTAATGTTGCTTCACTTCTTTTCAAGTATTTGTTATAATTGGCTGTATTTACATCGGTCAAAATGGACAGTTTCAGATGACTTTTCAAACCAACCACCATATTAAATTGTCGAACTCAAGTAATTAGTATATATTCCTTTAGTTGAAAGGTTCAAATTA

mRNA sequence

CTTTCAAATTGTTCCCCATCTCTCTTCCCTTTGTTCTTGTTGGAGCCAATTTCTCTGTAAACAAGATGCAGAAGCTCTTCGCTGCAAGGACTGCCTGTGCTTCCATTCTTAGGAGACAGAGACATTCTGCTTCTTTCTCTTCTGCTCTGCTTTTTGATGATACTCAACTACAGTTCAAAGAAAGTGTTAAACAATTTGCTCAAGATAACATTGCTCCCCATGCCGCAAAAATTGATCAAACAAACTCTTTTCCTCAGGATGTTAACTTATGGAAGCTCATGGGGGACTTCAATCTCCATGGAATTACTGCACCAGAGGAATATGGAGGACTTGGTCTGGGTTACTTGTATCACTGTATGGCAATGGAAGAAATAAGTCGCGCTTCTGGGTCTGTTGCCCTCTCCTTTGGTGCACATTCAAACCTGTGCATCAATCAGCTGGTGAGGAATGGAAACCCAGCTCAAAAAGAAAAGTACTTGCCTAAGCTTATTAGAGGGGAGCATGTGGGAGCTCTTGCAATGAGTGAACCCAATGCTGGCTCGGATGTTGTGAGCATGAAATGCAAAGCTGACCGGGTGGATGGTGGATATGTCATTAATGGAAACAAGATGTGGTGTACTAATGGTCCTACCGCTCAAACATTGGTTGTTTATGCAAAAACGGATGCAAATGCTGGCTCAAAGGGAATTACAGCATTTATCATCGAGAAAGGAATGCCCGGATTCAGTACTGCCCAGAAATTAGACAAACTTGGCATGCGAGGGAGTGATACATGTGAGCTTGTCTTTGAAAATTGCTTTGTTCCTGAAGAAAATATACTTGGACAAGAAGGAAAAGGAGTTTATGTTATGATGTCTGGGTTAGATTTGGAAAGGCTTGTTTTGGCTGCTGGTCCCCTAGGTCTTATGCAAGCATGCCTTGATGTTGTTCTTCCTTACGTTAAACAAAGAGAGCAGTTTGGTCGTCCAATTGGGGAATTCCAGTTTATACAGGGAAAACTTGCTGATATGTATACGTCTTTGCAATCATCAAGGGCCTATGTTTATTCTGTTGCTAGGGACTGTGACAGTGGAAAAGTTGATCCCAAGGATTGTGCTGGAGTAATATTATGTGCAGCTGAAAGAGCAACACAAGTTGCTCTCCAGGCCATACAATGCTTAGGAGGCAACGGATATGTAAACGAGTACGTAACCGGTCGACTACTTCGAGATGCAAAACTATATGAAATAGGTGCAGGTACTAGCGAAGTGAGAAGGATGATCATCGGTCGCTCACTGTTCAAAGAACAATAGGAGACAACTTTTACTCTCAATTGGATTGCTTCAGAGGAAACATCCCACTACCAAAGTCAATAATACTCAATACTCATCAACCCCCAGAGCAGGTTCTTAAGAAGCTAAGGTGATTGATGATTGAGGTCTTTATCTACGATGTGTAATTTTCAGGGAACATTGTTCAAATTTGTCATCATAATAATTGTTGCCTTTCATTGACATTGATAGAAAATAAAATGAAAGAAAGTCAATCAAATCAGGGAAAACGTGGTAATGTTGCTTCACTTCTTTTCAAGTATTTGTTATAATTGGCTGTATTTACATCGGTCAAAATGGACAGTTTCAGATGACTTTTCAAACCAACCACCATATTAAATTGTCGAACTCAAGTAATTAGTATATATTCCTTTAGTTGAAAGGTTCAAATTA

Coding sequence (CDS)

ATGCAGAAGCTCTTCGCTGCAAGGACTGCCTGTGCTTCCATTCTTAGGAGACAGAGACATTCTGCTTCTTTCTCTTCTGCTCTGCTTTTTGATGATACTCAACTACAGTTCAAAGAAAGTGTTAAACAATTTGCTCAAGATAACATTGCTCCCCATGCCGCAAAAATTGATCAAACAAACTCTTTTCCTCAGGATGTTAACTTATGGAAGCTCATGGGGGACTTCAATCTCCATGGAATTACTGCACCAGAGGAATATGGAGGACTTGGTCTGGGTTACTTGTATCACTGTATGGCAATGGAAGAAATAAGTCGCGCTTCTGGGTCTGTTGCCCTCTCCTTTGGTGCACATTCAAACCTGTGCATCAATCAGCTGGTGAGGAATGGAAACCCAGCTCAAAAAGAAAAGTACTTGCCTAAGCTTATTAGAGGGGAGCATGTGGGAGCTCTTGCAATGAGTGAACCCAATGCTGGCTCGGATGTTGTGAGCATGAAATGCAAAGCTGACCGGGTGGATGGTGGATATGTCATTAATGGAAACAAGATGTGGTGTACTAATGGTCCTACCGCTCAAACATTGGTTGTTTATGCAAAAACGGATGCAAATGCTGGCTCAAAGGGAATTACAGCATTTATCATCGAGAAAGGAATGCCCGGATTCAGTACTGCCCAGAAATTAGACAAACTTGGCATGCGAGGGAGTGATACATGTGAGCTTGTCTTTGAAAATTGCTTTGTTCCTGAAGAAAATATACTTGGACAAGAAGGAAAAGGAGTTTATGTTATGATGTCTGGGTTAGATTTGGAAAGGCTTGTTTTGGCTGCTGGTCCCCTAGGTCTTATGCAAGCATGCCTTGATGTTGTTCTTCCTTACGTTAAACAAAGAGAGCAGTTTGGTCGTCCAATTGGGGAATTCCAGTTTATACAGGGAAAACTTGCTGATATGTATACGTCTTTGCAATCATCAAGGGCCTATGTTTATTCTGTTGCTAGGGACTGTGACAGTGGAAAAGTTGATCCCAAGGATTGTGCTGGAGTAATATTATGTGCAGCTGAAAGAGCAACACAAGTTGCTCTCCAGGCCATACAATGCTTAGGAGGCAACGGATATGTAAACGAGTACGTAACCGGTCGACTACTTCGAGATGCAAAACTATATGAAATAGGTGCAGGTACTAGCGAAGTGAGAAGGATGATCATCGGTCGCTCACTGTTCAAAGAACAATAG

Protein sequence

MQKLFAARTACASILRRQRHSASFSSALLFDDTQLQFKESVKQFAQDNIAPHAAKIDQTNSFPQDVNLWKLMGDFNLHGITAPEEYGGLGLGYLYHCMAMEEISRASGSVALSFGAHSNLCINQLVRNGNPAQKEKYLPKLIRGEHVGALAMSEPNAGSDVVSMKCKADRVDGGYVINGNKMWCTNGPTAQTLVVYAKTDANAGSKGITAFIIEKGMPGFSTAQKLDKLGMRGSDTCELVFENCFVPEENILGQEGKGVYVMMSGLDLERLVLAAGPLGLMQACLDVVLPYVKQREQFGRPIGEFQFIQGKLADMYTSLQSSRAYVYSVARDCDSGKVDPKDCAGVILCAAERATQVALQAIQCLGGNGYVNEYVTGRLLRDAKLYEIGAGTSEVRRMIIGRSLFKEQ
Homology
BLAST of Lsi10G012850 vs. ExPASy Swiss-Prot
Match: Q9SWG0 (Isovaleryl-CoA dehydrogenase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=IVD PE=1 SV=2)

HSP 1 Score: 724.5 bits (1869), Expect = 6.5e-208
Identity = 349/409 (85.33%), Postives = 385/409 (94.13%), Query Frame = 0

Query: 1   MQKLFAARTACA-SILRRQRHSASFSSALLFDDTQLQFKESVKQFAQDNIAPHAAKIDQT 60
           MQ+ F+AR+    ++  R+R  +S SS+LLFDDTQLQFKESV +FAQDNIAPHA +ID+T
Sbjct: 1   MQRFFSARSILGYAVKTRRRSFSSRSSSLLFDDTQLQFKESVSKFAQDNIAPHAERIDKT 60

Query: 61  NSFPQDVNLWKLMGDFNLHGITAPEEYGGLGLGYLYHCMAMEEISRASGSVALSFGAHSN 120
           NSFP+DVNLWKLMG+FNLHGITAPEEYGGLGLGYLYHC+AMEEISRASGSVALS+GAHSN
Sbjct: 61  NSFPKDVNLWKLMGEFNLHGITAPEEYGGLGLGYLYHCIAMEEISRASGSVALSYGAHSN 120

Query: 121 LCINQLVRNGNPAQKEKYLPKLIRGEHVGALAMSEPNAGSDVVSMKCKADRVDGGYVING 180
           LCINQLVRNG  AQKEKYLPKLI GEHVGALAMSEPNAGSDVV MKCKA++VDGGY++NG
Sbjct: 121 LCINQLVRNGTAAQKEKYLPKLISGEHVGALAMSEPNAGSDVVGMKCKAEKVDGGYILNG 180

Query: 181 NKMWCTNGPTAQTLVVYAKTDANAGSKGITAFIIEKGMPGFSTAQKLDKLGMRGSDTCEL 240
           NKMWCTNGP+A+TLVVYAKTD  AGSKGITAFIIEKGM GFSTAQKLDKLGMRGSDTCEL
Sbjct: 181 NKMWCTNGPSAETLVVYAKTDTKAGSKGITAFIIEKGMTGFSTAQKLDKLGMRGSDTCEL 240

Query: 241 VFENCFVPEENILGQEGKGVYVMMSGLDLERLVLAAGPLGLMQACLDVVLPYVKQREQFG 300
           VFENCFVPEENIL +EGKGVYV+MSGLDLERLVLAAGPLG+MQACLD VLPY++QREQFG
Sbjct: 241 VFENCFVPEENILDKEGKGVYVLMSGLDLERLVLAAGPLGIMQACLDNVLPYIRQREQFG 300

Query: 301 RPIGEFQFIQGKLADMYTSLQSSRAYVYSVARDCDSGKVDPKDCAGVILCAAERATQVAL 360
           RP+GEFQFIQGK+ADMYT+LQSSR+YVYSVARDCD+GKVDPKDCAG ILCAAERATQVAL
Sbjct: 301 RPVGEFQFIQGKVADMYTALQSSRSYVYSVARDCDNGKVDPKDCAGTILCAAERATQVAL 360

Query: 361 QAIQCLGGNGYVNEYVTGRLLRDAKLYEIGAGTSEVRRMIIGRSLFKEQ 409
           QAIQCLGGNGY+NEY TGRLLRDAKLYEIGAGTSE+RR++IGR LFKE+
Sbjct: 361 QAIQCLGGNGYINEYATGRLLRDAKLYEIGAGTSEIRRIVIGRELFKEE 409

BLAST of Lsi10G012850 vs. ExPASy Swiss-Prot
Match: Q9FS87 (Isovaleryl-CoA dehydrogenase, mitochondrial OS=Solanum tuberosum OX=4113 GN=IVD PE=1 SV=2)

HSP 1 Score: 711.1 bits (1834), Expect = 7.4e-204
Identity = 347/412 (84.22%), Postives = 379/412 (91.99%), Query Frame = 0

Query: 1   MQKLFAARTACASILRRQRHS----ASFSSALLFDDTQLQFKESVKQFAQDNIAPHAAKI 60
           M KLF AR+  +++ R + H     A+FS++LLFDDTQ QFKESV QFAQ+NIAPHA KI
Sbjct: 1   MHKLFVARSVKSALFRIKNHQKPQFAAFSTSLLFDDTQKQFKESVAQFAQENIAPHAEKI 60

Query: 61  DQTNSFPQDVNLWKLMGDFNLHGITAPEEYGGLGLGYLYHCMAMEEISRASGSVALSFGA 120
           D+TN FPQDVNLWKLMG+FNL GIT PEEYGGLGLGYLYHC+AMEEISRASGSV LS+GA
Sbjct: 61  DRTNYFPQDVNLWKLMGNFNLLGITVPEEYGGLGLGYLYHCIAMEEISRASGSVGLSYGA 120

Query: 121 HSNLCINQLVRNGNPAQKEKYLPKLIRGEHVGALAMSEPNAGSDVVSMKCKADRVDGGYV 180
           H+NLCINQLVRNG   QK+KYLPKLI GEHVGALAMSEPNAGSDVVSMKCKADRV+GGYV
Sbjct: 121 HTNLCINQLVRNGTHEQKQKYLPKLISGEHVGALAMSEPNAGSDVVSMKCKADRVEGGYV 180

Query: 181 INGNKMWCTNGPTAQTLVVYAKTDANAGSKGITAFIIEKGMPGFSTAQKLDKLGMRGSDT 240
           +NGNKMWCTNGPTAQTLVVYAKTD  AGSKGITAFIIEKGM GFSTAQKLDKLGMRGSDT
Sbjct: 181 LNGNKMWCTNGPTAQTLVVYAKTDVTAGSKGITAFIIEKGMTGFSTAQKLDKLGMRGSDT 240

Query: 241 CELVFENCFVPEENILGQEGKGVYVMMSGLDLERLVLAAGPLGLMQACLDVVLPYVKQRE 300
           CELVFENCFVPEEN+LGQ G+GVYV+MSGLDLERLVLA+GP+G+MQACLDVVLPYVKQRE
Sbjct: 241 CELVFENCFVPEENVLGQVGRGVYVLMSGLDLERLVLASGPVGIMQACLDVVLPYVKQRE 300

Query: 301 QFGRPIGEFQFIQGKLADMYTSLQSSRAYVYSVARDCDSGKVDPKDCAGVILCAAERATQ 360
           QFGRPIGEFQF+QGK+ADMYTS+QSSR+Y+YSVAR+CDSG ++ KDCAGVIL AAERATQ
Sbjct: 301 QFGRPIGEFQFVQGKVADMYTSMQSSRSYLYSVARECDSGTINTKDCAGVILSAAERATQ 360

Query: 361 VALQAIQCLGGNGYVNEYVTGRLLRDAKLYEIGAGTSEVRRMIIGRSLFKEQ 409
           VALQAIQCLGGNGYVNEY TGR LRDAKLYEIGAGTSE+RRMIIGR LFKEQ
Sbjct: 361 VALQAIQCLGGNGYVNEYPTGRFLRDAKLYEIGAGTSEIRRMIIGRELFKEQ 412

BLAST of Lsi10G012850 vs. ExPASy Swiss-Prot
Match: Q75IM9 (Isovaleryl-CoA dehydrogenase, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0125500 PE=2 SV=2)

HSP 1 Score: 704.1 bits (1816), Expect = 9.0e-202
Identity = 346/407 (85.01%), Postives = 376/407 (92.38%), Query Frame = 0

Query: 6   AARTACASILRR----QRHSASFSSALLFDDTQLQFKESVKQFAQDNIAPHAAKIDQTNS 65
           AA+     ILRR     R   S +S+LLFDDTQ QFKESV +FAQ+ IAPHAA ID +N 
Sbjct: 3   AAQRWLPGILRRGDGLARRLYSSASSLLFDDTQEQFKESVHKFAQETIAPHAAAIDASNH 62

Query: 66  FPQDVNLWKLMGDFNLHGITAPEEYGGLGLGYLYHCMAMEEISRASGSVALSFGAHSNLC 125
           FP+DVNLWKLMGDFNLHG+TAPEEYGG+GLGY+YHC+AMEEISRASGSV LS+GAHSNLC
Sbjct: 63  FPKDVNLWKLMGDFNLHGLTAPEEYGGMGLGYMYHCIAMEEISRASGSVGLSYGAHSNLC 122

Query: 126 INQLVRNGNPAQKEKYLPKLIRGEHVGALAMSEPNAGSDVVSMKCKADRVDGGYVINGNK 185
           INQLVR+G+PAQK KYLPKLI GEHVGALAMSEPN+GSDVVSMKCKA++VDGGYVINGNK
Sbjct: 123 INQLVRHGSPAQKLKYLPKLISGEHVGALAMSEPNSGSDVVSMKCKAEKVDGGYVINGNK 182

Query: 186 MWCTNGPTAQTLVVYAKTDANAGSKGITAFIIEKGMPGFSTAQKLDKLGMRGSDTCELVF 245
           MWCTNGP+AQTLVVYAKTD  AGSKGITAFIIEKGMPGFSTAQKLDKLGMRGSDTCELVF
Sbjct: 183 MWCTNGPSAQTLVVYAKTDIAAGSKGITAFIIEKGMPGFSTAQKLDKLGMRGSDTCELVF 242

Query: 246 ENCFVPEENILGQEGKGVYVMMSGLDLERLVLAAGPLGLMQACLDVVLPYVKQREQFGRP 305
           ENCFVP EN+LG+EGKGVYVMMSGLDLERLVLAAGP+GLMQACLDV +PYV+QREQFGRP
Sbjct: 243 ENCFVPHENVLGEEGKGVYVMMSGLDLERLVLAAGPIGLMQACLDVAVPYVRQREQFGRP 302

Query: 306 IGEFQFIQGKLADMYTSLQSSRAYVYSVARDCDSGKVDPKDCAGVILCAAERATQVALQA 365
           IGEFQFIQGKLADMYTSLQSSR++VYSVARDCD+GKVD KDCAGVIL AAERATQVALQA
Sbjct: 303 IGEFQFIQGKLADMYTSLQSSRSFVYSVARDCDNGKVDRKDCAGVILFAAERATQVALQA 362

Query: 366 IQCLGGNGYVNEYVTGRLLRDAKLYEIGAGTSEVRRMIIGRSLFKEQ 409
           IQCLGGNGY+NEY TGRLLRDAKL+EIGAGTSE+RRMIIGR LFKE+
Sbjct: 363 IQCLGGNGYINEYPTGRLLRDAKLFEIGAGTSEIRRMIIGRELFKEE 409

BLAST of Lsi10G012850 vs. ExPASy Swiss-Prot
Match: Q9FS88 (2-methylacyl-CoA dehydrogenase, mitochondrial OS=Solanum tuberosum OX=4113 GN=2MBCD PE=1 SV=2)

HSP 1 Score: 697.2 bits (1798), Expect = 1.1e-199
Identity = 330/412 (80.10%), Postives = 377/412 (91.50%), Query Frame = 0

Query: 1   MQKLFAARTACASILRR----QRHSASFSSALLFDDTQLQFKESVKQFAQDNIAPHAAKI 60
           M KLFA R+  ++I++     Q   A+FS++LL DDTQ QFKESV +FAQ+NIAP+A KI
Sbjct: 1   MHKLFAVRSLSSAIVKSFKSLQNQQAAFSTSLLLDDTQKQFKESVAKFAQENIAPYAEKI 60

Query: 61  DQTNSFPQDVNLWKLMGDFNLHGITAPEEYGGLGLGYLYHCMAMEEISRASGSVALSFGA 120
           D+TNSFP+++NLWKLMGDFNLHGITAPEEYGGL LGYLYHC+A+EEISRASG+VA+S+G 
Sbjct: 61  DRTNSFPKEINLWKLMGDFNLHGITAPEEYGGLNLGYLYHCIALEEISRASGAVAVSYGV 120

Query: 121 HSNLCINQLVRNGNPAQKEKYLPKLIRGEHVGALAMSEPNAGSDVVSMKCKADRVDGGYV 180
            SN+CINQLVRNG P QK+KYLPKLI G+H+GALAMSEPNAGSDVVSMKC+ADRVDGGYV
Sbjct: 121 QSNVCINQLVRNGTPDQKQKYLPKLISGDHIGALAMSEPNAGSDVVSMKCRADRVDGGYV 180

Query: 181 INGNKMWCTNGPTAQTLVVYAKTDANAGSKGITAFIIEKGMPGFSTAQKLDKLGMRGSDT 240
           +NGNKMWCTNGP A TL+VYAKTD  AGSKGITAFIIEK MPGFSTAQKLDKLGMRGSDT
Sbjct: 181 LNGNKMWCTNGPVANTLIVYAKTDTTAGSKGITAFIIEKEMPGFSTAQKLDKLGMRGSDT 240

Query: 241 CELVFENCFVPEENILGQEGKGVYVMMSGLDLERLVLAAGPLGLMQACLDVVLPYVKQRE 300
           CELVFENCFVP EN+LGQEGKGVYV+MSGLDLERLVLAAGP+G+MQAC+D+V+PYV+QRE
Sbjct: 241 CELVFENCFVPNENVLGQEGKGVYVLMSGLDLERLVLAAGPVGIMQACMDIVIPYVRQRE 300

Query: 301 QFGRPIGEFQFIQGKLADMYTSLQSSRAYVYSVARDCDSGKVDPKDCAGVILCAAERATQ 360
           QFGRPIGEFQ IQGKLADMYT+LQSSR+YVY+VA+DCD+GK+DPKDC+G IL AAERATQ
Sbjct: 301 QFGRPIGEFQLIQGKLADMYTALQSSRSYVYAVAKDCDNGKIDPKDCSGTILLAAERATQ 360

Query: 361 VALQAIQCLGGNGYVNEYVTGRLLRDAKLYEIGAGTSEVRRMIIGRSLFKEQ 409
           VALQAIQCLGGNGY+NEY TGRLLRDAK+YEI AGTSE+RR++IGR LFK Q
Sbjct: 361 VALQAIQCLGGNGYINEYPTGRLLRDAKMYEIAAGTSEIRRLVIGRELFKHQ 412

BLAST of Lsi10G012850 vs. ExPASy Swiss-Prot
Match: Q9JHI5 (Isovaleryl-CoA dehydrogenase, mitochondrial OS=Mus musculus OX=10090 GN=Ivd PE=1 SV=1)

HSP 1 Score: 529.6 bits (1363), Expect = 3.0e-149
Identity = 257/375 (68.53%), Postives = 303/375 (80.80%), Query Frame = 0

Query: 31  DDTQLQFKESVKQFAQDNIAPHAAKIDQTNSFPQDVNLWKLMGDFNLHGITAPEEYGGLG 90
           ++ Q Q + ++ +F Q+N+AP A +IDQTN F      WK +G   + GITAP +YGG G
Sbjct: 44  NEEQKQLRHTISKFLQENLAPKAQEIDQTNDFKNLREFWKQLGSLGVLGITAPVQYGGSG 103

Query: 91  LGYLYHCMAMEEISRASGSVALSFGAHSNLCINQLVRNGNPAQKEKYLPKLIRGEHVGAL 150
           LGYL H + MEEISRASG+V LS+GAHSNLC+NQ+VRNGN AQKEKYLPKLI GE +GAL
Sbjct: 104 LGYLEHVLVMEEISRASGAVGLSYGAHSNLCVNQIVRNGNEAQKEKYLPKLISGEFIGAL 163

Query: 151 AMSEPNAGSDVVSMKCKADRVDGGYVINGNKMWCTNGPTAQTLVVYAKTDANA--GSKGI 210
           AMSEPNAGSDVVSMK KA++    YV+NGNK W TNGP A  LVVYAKTD  A   S+GI
Sbjct: 164 AMSEPNAGSDVVSMKLKAEKKGDHYVLNGNKFWITNGPDADILVVYAKTDLTAVPASRGI 223

Query: 211 TAFIIEKGMPGFSTAQKLDKLGMRGSDTCELVFENCFVPEENILGQEGKGVYVMMSGLDL 270
           TAFI+EKGMPGFST++KLDKLGMRGS+TCELVFE+C VP  N+L QE KGVYV+MSGLDL
Sbjct: 224 TAFIVEKGMPGFSTSKKLDKLGMRGSNTCELVFEDCKVPAANVLSQESKGVYVLMSGLDL 283

Query: 271 ERLVLAAGPLGLMQACLDVVLPYVKQREQFGRPIGEFQFIQGKLADMYTSLQSSRAYVYS 330
           ERLVLA GPLG+MQA LD  +PY+  RE FG+ IG+FQ +QGK+ADMYT L +SR YVY+
Sbjct: 284 ERLVLAGGPLGIMQAVLDHTIPYLHVREAFGQKIGQFQLMQGKMADMYTRLMASRQYVYN 343

Query: 331 VARDCDSGKVDPKDCAGVILCAAERATQVALQAIQCLGGNGYVNEYVTGRLLRDAKLYEI 390
           VA+ CD G + PKDCAGVIL AAE ATQVAL  IQCLGGNGY+N++  GR LRDAKLYEI
Sbjct: 344 VAKACDEGHIIPKDCAGVILYAAECATQVALDGIQCLGGNGYINDFPMGRFLRDAKLYEI 403

Query: 391 GAGTSEVRRMIIGRS 404
           GAGTSEVRR++IGR+
Sbjct: 404 GAGTSEVRRLVIGRA 418

BLAST of Lsi10G012850 vs. ExPASy TrEMBL
Match: A0A5D3CRA1 (Isovaleryl-CoA dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84G00370 PE=3 SV=1)

HSP 1 Score: 807.0 bits (2083), Expect = 3.7e-230
Identity = 397/408 (97.30%), Postives = 405/408 (99.26%), Query Frame = 0

Query: 1   MQKLFAARTACASILRRQRHSASFSSALLFDDTQLQFKESVKQFAQDNIAPHAAKIDQTN 60
           MQKLFAA TACASILRRQRHSASFSSALLFDDTQ+QFKESVKQFAQ+NIAPHAAKIDQTN
Sbjct: 1   MQKLFAATTACASILRRQRHSASFSSALLFDDTQIQFKESVKQFAQENIAPHAAKIDQTN 60

Query: 61  SFPQDVNLWKLMGDFNLHGITAPEEYGGLGLGYLYHCMAMEEISRASGSVALSFGAHSNL 120
           SFP+DVNLWKLMGDF+LHGITAPEEYGGLGLGYLYHCMAMEEISRASGSVALSFGAHSNL
Sbjct: 61  SFPKDVNLWKLMGDFDLHGITAPEEYGGLGLGYLYHCMAMEEISRASGSVALSFGAHSNL 120

Query: 121 CINQLVRNGNPAQKEKYLPKLIRGEHVGALAMSEPNAGSDVVSMKCKADRVDGGYVINGN 180
           CINQLVRNGNPAQKEKYLPKLI+GEHVGALAMSEPNAGSDVVSMKCKADRVDGGYVINGN
Sbjct: 121 CINQLVRNGNPAQKEKYLPKLIKGEHVGALAMSEPNAGSDVVSMKCKADRVDGGYVINGN 180

Query: 181 KMWCTNGPTAQTLVVYAKTDANAGSKGITAFIIEKGMPGFSTAQKLDKLGMRGSDTCELV 240
           KMWCTNGPTAQTL+VYAKTD NAGSKGITAFIIEKGMPGFSTAQKLDKLGMRGSDTCELV
Sbjct: 181 KMWCTNGPTAQTLIVYAKTDPNAGSKGITAFIIEKGMPGFSTAQKLDKLGMRGSDTCELV 240

Query: 241 FENCFVPEENILGQEGKGVYVMMSGLDLERLVLAAGPLGLMQACLDVVLPYVKQREQFGR 300
           FENCFVPEEN+LGQEGKGVYVMMSGLDLERLVLAAGPLGLMQACLD VLPYVKQREQFGR
Sbjct: 241 FENCFVPEENVLGQEGKGVYVMMSGLDLERLVLAAGPLGLMQACLDAVLPYVKQREQFGR 300

Query: 301 PIGEFQFIQGKLADMYTSLQSSRAYVYSVARDCDSGKVDPKDCAGVILCAAERATQVALQ 360
           PIGEFQFIQGKLADMYTSLQSSRAYVYSVARDCD+GKVDPKDCAGVILCAAERATQVALQ
Sbjct: 301 PIGEFQFIQGKLADMYTSLQSSRAYVYSVARDCDNGKVDPKDCAGVILCAAERATQVALQ 360

Query: 361 AIQCLGGNGYVNEYVTGRLLRDAKLYEIGAGTSEVRRMIIGRSLFKEQ 409
           AIQCLGGNGYVNEYVTGRLLRDAKLYEIGAGTSEVRRMIIGRSLFKEQ
Sbjct: 361 AIQCLGGNGYVNEYVTGRLLRDAKLYEIGAGTSEVRRMIIGRSLFKEQ 408

BLAST of Lsi10G012850 vs. ExPASy TrEMBL
Match: A0A1S3CKE1 (isovaleryl-CoA dehydrogenase, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103501934 PE=3 SV=1)

HSP 1 Score: 807.0 bits (2083), Expect = 3.7e-230
Identity = 397/408 (97.30%), Postives = 405/408 (99.26%), Query Frame = 0

Query: 1   MQKLFAARTACASILRRQRHSASFSSALLFDDTQLQFKESVKQFAQDNIAPHAAKIDQTN 60
           MQKLFAA TACASILRRQRHSASFSSALLFDDTQ+QFKESVKQFAQ+NIAPHAAKIDQTN
Sbjct: 1   MQKLFAATTACASILRRQRHSASFSSALLFDDTQIQFKESVKQFAQENIAPHAAKIDQTN 60

Query: 61  SFPQDVNLWKLMGDFNLHGITAPEEYGGLGLGYLYHCMAMEEISRASGSVALSFGAHSNL 120
           SFP+DVNLWKLMGDF+LHGITAPEEYGGLGLGYLYHCMAMEEISRASGSVALSFGAHSNL
Sbjct: 61  SFPKDVNLWKLMGDFDLHGITAPEEYGGLGLGYLYHCMAMEEISRASGSVALSFGAHSNL 120

Query: 121 CINQLVRNGNPAQKEKYLPKLIRGEHVGALAMSEPNAGSDVVSMKCKADRVDGGYVINGN 180
           CINQLVRNGNPAQKEKYLPKLI+GEHVGALAMSEPNAGSDVVSMKCKADRVDGGYVINGN
Sbjct: 121 CINQLVRNGNPAQKEKYLPKLIKGEHVGALAMSEPNAGSDVVSMKCKADRVDGGYVINGN 180

Query: 181 KMWCTNGPTAQTLVVYAKTDANAGSKGITAFIIEKGMPGFSTAQKLDKLGMRGSDTCELV 240
           KMWCTNGPTAQTL+VYAKTD NAGSKGITAFIIEKGMPGFSTAQKLDKLGMRGSDTCELV
Sbjct: 181 KMWCTNGPTAQTLIVYAKTDPNAGSKGITAFIIEKGMPGFSTAQKLDKLGMRGSDTCELV 240

Query: 241 FENCFVPEENILGQEGKGVYVMMSGLDLERLVLAAGPLGLMQACLDVVLPYVKQREQFGR 300
           FENCFVPEEN+LGQEGKGVYVMMSGLDLERLVLAAGPLGLMQACLD VLPYVKQREQFGR
Sbjct: 241 FENCFVPEENVLGQEGKGVYVMMSGLDLERLVLAAGPLGLMQACLDAVLPYVKQREQFGR 300

Query: 301 PIGEFQFIQGKLADMYTSLQSSRAYVYSVARDCDSGKVDPKDCAGVILCAAERATQVALQ 360
           PIGEFQFIQGKLADMYTSLQSSRAYVYSVARDCD+GKVDPKDCAGVILCAAERATQVALQ
Sbjct: 301 PIGEFQFIQGKLADMYTSLQSSRAYVYSVARDCDNGKVDPKDCAGVILCAAERATQVALQ 360

Query: 361 AIQCLGGNGYVNEYVTGRLLRDAKLYEIGAGTSEVRRMIIGRSLFKEQ 409
           AIQCLGGNGYVNEYVTGRLLRDAKLYEIGAGTSEVRRMIIGRSLFKEQ
Sbjct: 361 AIQCLGGNGYVNEYVTGRLLRDAKLYEIGAGTSEVRRMIIGRSLFKEQ 408

BLAST of Lsi10G012850 vs. ExPASy TrEMBL
Match: A0A6J1CIC1 (isovaleryl-CoA dehydrogenase, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111011209 PE=3 SV=1)

HSP 1 Score: 802.7 bits (2072), Expect = 6.9e-229
Identity = 395/408 (96.81%), Postives = 404/408 (99.02%), Query Frame = 0

Query: 1   MQKLFAARTACASILRRQRHSASFSSALLFDDTQLQFKESVKQFAQDNIAPHAAKIDQTN 60
           MQ+LFAARTACA+ILRRQR SASFSSALLFDDTQLQFKESVKQFAQDNIAPHA+ IDQTN
Sbjct: 1   MQRLFAARTACAAILRRQRQSASFSSALLFDDTQLQFKESVKQFAQDNIAPHASIIDQTN 60

Query: 61  SFPQDVNLWKLMGDFNLHGITAPEEYGGLGLGYLYHCMAMEEISRASGSVALSFGAHSNL 120
           SFPQDVNLWKLMGDFNLHGITAPEEYGGLGLGYLYHC+AMEEISRASGSVALSFGAHSNL
Sbjct: 61  SFPQDVNLWKLMGDFNLHGITAPEEYGGLGLGYLYHCIAMEEISRASGSVALSFGAHSNL 120

Query: 121 CINQLVRNGNPAQKEKYLPKLIRGEHVGALAMSEPNAGSDVVSMKCKADRVDGGYVINGN 180
           CINQLVRNG+PAQKEKYLPKLIRGEHVGALAMSEPNAGSDVVSMKCKADRVDGGY+INGN
Sbjct: 121 CINQLVRNGSPAQKEKYLPKLIRGEHVGALAMSEPNAGSDVVSMKCKADRVDGGYIINGN 180

Query: 181 KMWCTNGPTAQTLVVYAKTDANAGSKGITAFIIEKGMPGFSTAQKLDKLGMRGSDTCELV 240
           KMWCTNGPTAQTLVVYAKTD NAGSKGITAFIIEKGMPGFSTAQKLDKLGMRGSDTCELV
Sbjct: 181 KMWCTNGPTAQTLVVYAKTDTNAGSKGITAFIIEKGMPGFSTAQKLDKLGMRGSDTCELV 240

Query: 241 FENCFVPEENILGQEGKGVYVMMSGLDLERLVLAAGPLGLMQACLDVVLPYVKQREQFGR 300
           FENCFVPEEN+LGQEGKGVYVMMSGLDLERLVLAAGPLG+MQACLD VLPYVKQREQFGR
Sbjct: 241 FENCFVPEENVLGQEGKGVYVMMSGLDLERLVLAAGPLGIMQACLDAVLPYVKQREQFGR 300

Query: 301 PIGEFQFIQGKLADMYTSLQSSRAYVYSVARDCDSGKVDPKDCAGVILCAAERATQVALQ 360
           PIGEFQFIQGKLADMYTSLQSSR+YVYSVARDCDSGKVDPKDCAGVILCAAERATQVALQ
Sbjct: 301 PIGEFQFIQGKLADMYTSLQSSRSYVYSVARDCDSGKVDPKDCAGVILCAAERATQVALQ 360

Query: 361 AIQCLGGNGYVNEYVTGRLLRDAKLYEIGAGTSEVRRMIIGRSLFKEQ 409
           AIQCLGGNGYVNEYVTGRLLRDAKLYEIGAGTSEVRRMIIGRSLFKEQ
Sbjct: 361 AIQCLGGNGYVNEYVTGRLLRDAKLYEIGAGTSEVRRMIIGRSLFKEQ 408

BLAST of Lsi10G012850 vs. ExPASy TrEMBL
Match: A0A6J1H1Y4 (isovaleryl-CoA dehydrogenase, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111459300 PE=3 SV=1)

HSP 1 Score: 799.3 bits (2063), Expect = 7.6e-228
Identity = 396/409 (96.82%), Postives = 404/409 (98.78%), Query Frame = 0

Query: 1   MQKLFAARTAC-ASILRRQRHSASFSSALLFDDTQLQFKESVKQFAQDNIAPHAAKIDQT 60
           MQ+LFAARTA  ASI R+QRHSASFSSALLFDDTQLQFKESVKQFAQDNIAPHAAKIDQ 
Sbjct: 1   MQRLFAARTAAYASIFRKQRHSASFSSALLFDDTQLQFKESVKQFAQDNIAPHAAKIDQM 60

Query: 61  NSFPQDVNLWKLMGDFNLHGITAPEEYGGLGLGYLYHCMAMEEISRASGSVALSFGAHSN 120
           NSFPQDVNLWKLMGDFNLHGITAPEEYGGLGLGYLYHC+AMEEISRASGSVALSFGAHSN
Sbjct: 61  NSFPQDVNLWKLMGDFNLHGITAPEEYGGLGLGYLYHCIAMEEISRASGSVALSFGAHSN 120

Query: 121 LCINQLVRNGNPAQKEKYLPKLIRGEHVGALAMSEPNAGSDVVSMKCKADRVDGGYVING 180
           LCINQLVRNG+PAQKEKYLPKLI+GEHVGALAMSEPNAGSDVVSMKCKADRVDGGY+ING
Sbjct: 121 LCINQLVRNGSPAQKEKYLPKLIKGEHVGALAMSEPNAGSDVVSMKCKADRVDGGYIING 180

Query: 181 NKMWCTNGPTAQTLVVYAKTDANAGSKGITAFIIEKGMPGFSTAQKLDKLGMRGSDTCEL 240
           NKMWCTNGPTAQTLVVYAKTDANAGSKGITAFIIEKGMPGFSTAQKLDKLGMRGSDTCEL
Sbjct: 181 NKMWCTNGPTAQTLVVYAKTDANAGSKGITAFIIEKGMPGFSTAQKLDKLGMRGSDTCEL 240

Query: 241 VFENCFVPEENILGQEGKGVYVMMSGLDLERLVLAAGPLGLMQACLDVVLPYVKQREQFG 300
           VFENCFVPEEN+LGQEGKGVYVMMSGLDLERLVLAAGPLGLMQACLDVVLPYVKQREQFG
Sbjct: 241 VFENCFVPEENVLGQEGKGVYVMMSGLDLERLVLAAGPLGLMQACLDVVLPYVKQREQFG 300

Query: 301 RPIGEFQFIQGKLADMYTSLQSSRAYVYSVARDCDSGKVDPKDCAGVILCAAERATQVAL 360
           RPIGEFQFIQGKLADMYTSLQSSR+YVYSVARDCDSGKVDPKDCAGVILCAAERATQVAL
Sbjct: 301 RPIGEFQFIQGKLADMYTSLQSSRSYVYSVARDCDSGKVDPKDCAGVILCAAERATQVAL 360

Query: 361 QAIQCLGGNGYVNEYVTGRLLRDAKLYEIGAGTSEVRRMIIGRSLFKEQ 409
           QAIQCLGGNGYVNEY TGRLLRDAKLYEIGAGTSEVRRMIIGRSLFKEQ
Sbjct: 361 QAIQCLGGNGYVNEYTTGRLLRDAKLYEIGAGTSEVRRMIIGRSLFKEQ 409

BLAST of Lsi10G012850 vs. ExPASy TrEMBL
Match: A0A6J1K1P8 (isovaleryl-CoA dehydrogenase, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111490920 PE=3 SV=1)

HSP 1 Score: 794.7 bits (2051), Expect = 1.9e-226
Identity = 395/409 (96.58%), Postives = 403/409 (98.53%), Query Frame = 0

Query: 1   MQKLFAART-ACASILRRQRHSASFSSALLFDDTQLQFKESVKQFAQDNIAPHAAKIDQT 60
           MQ+LFAART A ASI R+QRHSASFSSALLFDDTQLQFKESVKQFAQDNIAPHAAKIDQ 
Sbjct: 1   MQRLFAARTVAYASIFRKQRHSASFSSALLFDDTQLQFKESVKQFAQDNIAPHAAKIDQM 60

Query: 61  NSFPQDVNLWKLMGDFNLHGITAPEEYGGLGLGYLYHCMAMEEISRASGSVALSFGAHSN 120
           NSFPQDVNLWKLMGDFNLHGITAPEEYGGLGLGYLYHC+AMEEISRASGSVALSFGAHSN
Sbjct: 61  NSFPQDVNLWKLMGDFNLHGITAPEEYGGLGLGYLYHCIAMEEISRASGSVALSFGAHSN 120

Query: 121 LCINQLVRNGNPAQKEKYLPKLIRGEHVGALAMSEPNAGSDVVSMKCKADRVDGGYVING 180
           LCINQLVRNG+PAQKEKYLPKLI+GEHVGALAMSEPNAGSDVVSMKC+ADRVDGGY+ING
Sbjct: 121 LCINQLVRNGSPAQKEKYLPKLIKGEHVGALAMSEPNAGSDVVSMKCRADRVDGGYIING 180

Query: 181 NKMWCTNGPTAQTLVVYAKTDANAGSKGITAFIIEKGMPGFSTAQKLDKLGMRGSDTCEL 240
           NKMWCTNGPTAQTLVVYAKTDANAGSKGITAFIIEKGMPGFSTAQKLDKLGMRGSDTCEL
Sbjct: 181 NKMWCTNGPTAQTLVVYAKTDANAGSKGITAFIIEKGMPGFSTAQKLDKLGMRGSDTCEL 240

Query: 241 VFENCFVPEENILGQEGKGVYVMMSGLDLERLVLAAGPLGLMQACLDVVLPYVKQREQFG 300
           VFENCFVPEEN+LGQEGKGVYVMMSGLDLERLVLAAGPLGLMQACLDVVLPYVKQREQFG
Sbjct: 241 VFENCFVPEENVLGQEGKGVYVMMSGLDLERLVLAAGPLGLMQACLDVVLPYVKQREQFG 300

Query: 301 RPIGEFQFIQGKLADMYTSLQSSRAYVYSVARDCDSGKVDPKDCAGVILCAAERATQVAL 360
           RPIGEFQFIQGKLADMYTSLQSSRAYVYSVARDCDSGKVDPKDCAGVIL AAERATQVAL
Sbjct: 301 RPIGEFQFIQGKLADMYTSLQSSRAYVYSVARDCDSGKVDPKDCAGVILSAAERATQVAL 360

Query: 361 QAIQCLGGNGYVNEYVTGRLLRDAKLYEIGAGTSEVRRMIIGRSLFKEQ 409
           QAIQCLGGNGYVNEY TGRLLRDAKLYEIGAGTSEVRRMIIGRSLFKEQ
Sbjct: 361 QAIQCLGGNGYVNEYTTGRLLRDAKLYEIGAGTSEVRRMIIGRSLFKEQ 409

BLAST of Lsi10G012850 vs. NCBI nr
Match: XP_038901894.1 (isovaleryl-CoA dehydrogenase, mitochondrial [Benincasa hispida])

HSP 1 Score: 814.7 bits (2103), Expect = 3.6e-232
Identity = 403/408 (98.77%), Postives = 407/408 (99.75%), Query Frame = 0

Query: 1   MQKLFAARTACASILRRQRHSASFSSALLFDDTQLQFKESVKQFAQDNIAPHAAKIDQTN 60
           MQKLFAARTACASILRRQ HSASFSSALLFDDTQLQFKESVKQFAQDNIAPHAAKIDQTN
Sbjct: 1   MQKLFAARTACASILRRQGHSASFSSALLFDDTQLQFKESVKQFAQDNIAPHAAKIDQTN 60

Query: 61  SFPQDVNLWKLMGDFNLHGITAPEEYGGLGLGYLYHCMAMEEISRASGSVALSFGAHSNL 120
           SFPQDVNLWKLMGDFNLHGITAPEEYGGLGLGYLYHCMAMEEISRASGSVALSFGAHSNL
Sbjct: 61  SFPQDVNLWKLMGDFNLHGITAPEEYGGLGLGYLYHCMAMEEISRASGSVALSFGAHSNL 120

Query: 121 CINQLVRNGNPAQKEKYLPKLIRGEHVGALAMSEPNAGSDVVSMKCKADRVDGGYVINGN 180
           CINQLVRNGNPAQKEKYLPKLI+G+HVGALAMSEPNAGSDVVSMKCKAD+VDGGYVINGN
Sbjct: 121 CINQLVRNGNPAQKEKYLPKLIKGDHVGALAMSEPNAGSDVVSMKCKADQVDGGYVINGN 180

Query: 181 KMWCTNGPTAQTLVVYAKTDANAGSKGITAFIIEKGMPGFSTAQKLDKLGMRGSDTCELV 240
           KMWCTNGPTAQTLVVYAKTDANAGSKGITAFIIEKGMPGFSTAQKLDKLGMRGSDTCELV
Sbjct: 181 KMWCTNGPTAQTLVVYAKTDANAGSKGITAFIIEKGMPGFSTAQKLDKLGMRGSDTCELV 240

Query: 241 FENCFVPEENILGQEGKGVYVMMSGLDLERLVLAAGPLGLMQACLDVVLPYVKQREQFGR 300
           FENCFVPEEN+LGQEGKGVYVMMSGLDLERLVLAAGPLGLMQACLDVVLPYVKQREQFGR
Sbjct: 241 FENCFVPEENVLGQEGKGVYVMMSGLDLERLVLAAGPLGLMQACLDVVLPYVKQREQFGR 300

Query: 301 PIGEFQFIQGKLADMYTSLQSSRAYVYSVARDCDSGKVDPKDCAGVILCAAERATQVALQ 360
           PIGEFQFIQGKLADMYTSLQSSRAYVYSVARDCDSGKVDPKDCAGVILCAAERATQVALQ
Sbjct: 301 PIGEFQFIQGKLADMYTSLQSSRAYVYSVARDCDSGKVDPKDCAGVILCAAERATQVALQ 360

Query: 361 AIQCLGGNGYVNEYVTGRLLRDAKLYEIGAGTSEVRRMIIGRSLFKEQ 409
           AIQCLGGNGYVNEYVTGRLLRDAKLYEIGAGTSEVRRMIIGRSLFKEQ
Sbjct: 361 AIQCLGGNGYVNEYVTGRLLRDAKLYEIGAGTSEVRRMIIGRSLFKEQ 408

BLAST of Lsi10G012850 vs. NCBI nr
Match: XP_008463922.1 (PREDICTED: isovaleryl-CoA dehydrogenase, mitochondrial [Cucumis melo] >KAA0035326.1 isovaleryl-CoA dehydrogenase [Cucumis melo var. makuwa] >TYK14311.1 isovaleryl-CoA dehydrogenase [Cucumis melo var. makuwa])

HSP 1 Score: 807.0 bits (2083), Expect = 7.5e-230
Identity = 397/408 (97.30%), Postives = 405/408 (99.26%), Query Frame = 0

Query: 1   MQKLFAARTACASILRRQRHSASFSSALLFDDTQLQFKESVKQFAQDNIAPHAAKIDQTN 60
           MQKLFAA TACASILRRQRHSASFSSALLFDDTQ+QFKESVKQFAQ+NIAPHAAKIDQTN
Sbjct: 1   MQKLFAATTACASILRRQRHSASFSSALLFDDTQIQFKESVKQFAQENIAPHAAKIDQTN 60

Query: 61  SFPQDVNLWKLMGDFNLHGITAPEEYGGLGLGYLYHCMAMEEISRASGSVALSFGAHSNL 120
           SFP+DVNLWKLMGDF+LHGITAPEEYGGLGLGYLYHCMAMEEISRASGSVALSFGAHSNL
Sbjct: 61  SFPKDVNLWKLMGDFDLHGITAPEEYGGLGLGYLYHCMAMEEISRASGSVALSFGAHSNL 120

Query: 121 CINQLVRNGNPAQKEKYLPKLIRGEHVGALAMSEPNAGSDVVSMKCKADRVDGGYVINGN 180
           CINQLVRNGNPAQKEKYLPKLI+GEHVGALAMSEPNAGSDVVSMKCKADRVDGGYVINGN
Sbjct: 121 CINQLVRNGNPAQKEKYLPKLIKGEHVGALAMSEPNAGSDVVSMKCKADRVDGGYVINGN 180

Query: 181 KMWCTNGPTAQTLVVYAKTDANAGSKGITAFIIEKGMPGFSTAQKLDKLGMRGSDTCELV 240
           KMWCTNGPTAQTL+VYAKTD NAGSKGITAFIIEKGMPGFSTAQKLDKLGMRGSDTCELV
Sbjct: 181 KMWCTNGPTAQTLIVYAKTDPNAGSKGITAFIIEKGMPGFSTAQKLDKLGMRGSDTCELV 240

Query: 241 FENCFVPEENILGQEGKGVYVMMSGLDLERLVLAAGPLGLMQACLDVVLPYVKQREQFGR 300
           FENCFVPEEN+LGQEGKGVYVMMSGLDLERLVLAAGPLGLMQACLD VLPYVKQREQFGR
Sbjct: 241 FENCFVPEENVLGQEGKGVYVMMSGLDLERLVLAAGPLGLMQACLDAVLPYVKQREQFGR 300

Query: 301 PIGEFQFIQGKLADMYTSLQSSRAYVYSVARDCDSGKVDPKDCAGVILCAAERATQVALQ 360
           PIGEFQFIQGKLADMYTSLQSSRAYVYSVARDCD+GKVDPKDCAGVILCAAERATQVALQ
Sbjct: 301 PIGEFQFIQGKLADMYTSLQSSRAYVYSVARDCDNGKVDPKDCAGVILCAAERATQVALQ 360

Query: 361 AIQCLGGNGYVNEYVTGRLLRDAKLYEIGAGTSEVRRMIIGRSLFKEQ 409
           AIQCLGGNGYVNEYVTGRLLRDAKLYEIGAGTSEVRRMIIGRSLFKEQ
Sbjct: 361 AIQCLGGNGYVNEYVTGRLLRDAKLYEIGAGTSEVRRMIIGRSLFKEQ 408

BLAST of Lsi10G012850 vs. NCBI nr
Match: XP_022140593.1 (isovaleryl-CoA dehydrogenase, mitochondrial [Momordica charantia])

HSP 1 Score: 802.7 bits (2072), Expect = 1.4e-228
Identity = 395/408 (96.81%), Postives = 404/408 (99.02%), Query Frame = 0

Query: 1   MQKLFAARTACASILRRQRHSASFSSALLFDDTQLQFKESVKQFAQDNIAPHAAKIDQTN 60
           MQ+LFAARTACA+ILRRQR SASFSSALLFDDTQLQFKESVKQFAQDNIAPHA+ IDQTN
Sbjct: 1   MQRLFAARTACAAILRRQRQSASFSSALLFDDTQLQFKESVKQFAQDNIAPHASIIDQTN 60

Query: 61  SFPQDVNLWKLMGDFNLHGITAPEEYGGLGLGYLYHCMAMEEISRASGSVALSFGAHSNL 120
           SFPQDVNLWKLMGDFNLHGITAPEEYGGLGLGYLYHC+AMEEISRASGSVALSFGAHSNL
Sbjct: 61  SFPQDVNLWKLMGDFNLHGITAPEEYGGLGLGYLYHCIAMEEISRASGSVALSFGAHSNL 120

Query: 121 CINQLVRNGNPAQKEKYLPKLIRGEHVGALAMSEPNAGSDVVSMKCKADRVDGGYVINGN 180
           CINQLVRNG+PAQKEKYLPKLIRGEHVGALAMSEPNAGSDVVSMKCKADRVDGGY+INGN
Sbjct: 121 CINQLVRNGSPAQKEKYLPKLIRGEHVGALAMSEPNAGSDVVSMKCKADRVDGGYIINGN 180

Query: 181 KMWCTNGPTAQTLVVYAKTDANAGSKGITAFIIEKGMPGFSTAQKLDKLGMRGSDTCELV 240
           KMWCTNGPTAQTLVVYAKTD NAGSKGITAFIIEKGMPGFSTAQKLDKLGMRGSDTCELV
Sbjct: 181 KMWCTNGPTAQTLVVYAKTDTNAGSKGITAFIIEKGMPGFSTAQKLDKLGMRGSDTCELV 240

Query: 241 FENCFVPEENILGQEGKGVYVMMSGLDLERLVLAAGPLGLMQACLDVVLPYVKQREQFGR 300
           FENCFVPEEN+LGQEGKGVYVMMSGLDLERLVLAAGPLG+MQACLD VLPYVKQREQFGR
Sbjct: 241 FENCFVPEENVLGQEGKGVYVMMSGLDLERLVLAAGPLGIMQACLDAVLPYVKQREQFGR 300

Query: 301 PIGEFQFIQGKLADMYTSLQSSRAYVYSVARDCDSGKVDPKDCAGVILCAAERATQVALQ 360
           PIGEFQFIQGKLADMYTSLQSSR+YVYSVARDCDSGKVDPKDCAGVILCAAERATQVALQ
Sbjct: 301 PIGEFQFIQGKLADMYTSLQSSRSYVYSVARDCDSGKVDPKDCAGVILCAAERATQVALQ 360

Query: 361 AIQCLGGNGYVNEYVTGRLLRDAKLYEIGAGTSEVRRMIIGRSLFKEQ 409
           AIQCLGGNGYVNEYVTGRLLRDAKLYEIGAGTSEVRRMIIGRSLFKEQ
Sbjct: 361 AIQCLGGNGYVNEYVTGRLLRDAKLYEIGAGTSEVRRMIIGRSLFKEQ 408

BLAST of Lsi10G012850 vs. NCBI nr
Match: XP_011657216.1 (isovaleryl-CoA dehydrogenase, mitochondrial [Cucumis sativus] >KAE8647055.1 hypothetical protein Csa_022979 [Cucumis sativus])

HSP 1 Score: 800.4 bits (2066), Expect = 7.1e-228
Identity = 394/408 (96.57%), Postives = 402/408 (98.53%), Query Frame = 0

Query: 1   MQKLFAARTACASILRRQRHSASFSSALLFDDTQLQFKESVKQFAQDNIAPHAAKIDQTN 60
           MQKLFAA TACASILR  RHSASFSSALLFDDTQLQFKESVKQFAQDNIAPHAAKIDQTN
Sbjct: 1   MQKLFAATTACASILRTHRHSASFSSALLFDDTQLQFKESVKQFAQDNIAPHAAKIDQTN 60

Query: 61  SFPQDVNLWKLMGDFNLHGITAPEEYGGLGLGYLYHCMAMEEISRASGSVALSFGAHSNL 120
           +FP+DVNLWKLMGDF+LHGITAPEEYGGLGLGYLYHCMAMEEISRASGSVALSFGAHSNL
Sbjct: 61  TFPKDVNLWKLMGDFHLHGITAPEEYGGLGLGYLYHCMAMEEISRASGSVALSFGAHSNL 120

Query: 121 CINQLVRNGNPAQKEKYLPKLIRGEHVGALAMSEPNAGSDVVSMKCKADRVDGGYVINGN 180
           CINQLVRNGNP QKEKYLPKLI+GEHVGALAMSEPNAGSDVVSMKCKADRVDGGY+INGN
Sbjct: 121 CINQLVRNGNPTQKEKYLPKLIKGEHVGALAMSEPNAGSDVVSMKCKADRVDGGYIINGN 180

Query: 181 KMWCTNGPTAQTLVVYAKTDANAGSKGITAFIIEKGMPGFSTAQKLDKLGMRGSDTCELV 240
           KMWCTNGPTAQTLVVYAKT+ NAGSKGITAFIIEKGMPGFSTAQKLDKLGMRGSDTCELV
Sbjct: 181 KMWCTNGPTAQTLVVYAKTNPNAGSKGITAFIIEKGMPGFSTAQKLDKLGMRGSDTCELV 240

Query: 241 FENCFVPEENILGQEGKGVYVMMSGLDLERLVLAAGPLGLMQACLDVVLPYVKQREQFGR 300
           FENCFVPEEN+LGQEGKGVYVMMSGLDLERLVLAAGPLGLMQACLD VLPYVKQREQFGR
Sbjct: 241 FENCFVPEENVLGQEGKGVYVMMSGLDLERLVLAAGPLGLMQACLDAVLPYVKQREQFGR 300

Query: 301 PIGEFQFIQGKLADMYTSLQSSRAYVYSVARDCDSGKVDPKDCAGVILCAAERATQVALQ 360
           PIGEFQFIQGKLADMYTSLQSSRAYVYSVARDCD+GKVDPKDCAGVILCAAERATQVALQ
Sbjct: 301 PIGEFQFIQGKLADMYTSLQSSRAYVYSVARDCDNGKVDPKDCAGVILCAAERATQVALQ 360

Query: 361 AIQCLGGNGYVNEYVTGRLLRDAKLYEIGAGTSEVRRMIIGRSLFKEQ 409
           AIQCLGGNGYVNEYVTGRLLRDAKLYEIGAGTSEVRRMIIGRSLFKEQ
Sbjct: 361 AIQCLGGNGYVNEYVTGRLLRDAKLYEIGAGTSEVRRMIIGRSLFKEQ 408

BLAST of Lsi10G012850 vs. NCBI nr
Match: XP_022957905.1 (isovaleryl-CoA dehydrogenase, mitochondrial [Cucurbita moschata] >KAG7013907.1 Isovaleryl-CoA dehydrogenase, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 799.3 bits (2063), Expect = 1.6e-227
Identity = 396/409 (96.82%), Postives = 404/409 (98.78%), Query Frame = 0

Query: 1   MQKLFAARTAC-ASILRRQRHSASFSSALLFDDTQLQFKESVKQFAQDNIAPHAAKIDQT 60
           MQ+LFAARTA  ASI R+QRHSASFSSALLFDDTQLQFKESVKQFAQDNIAPHAAKIDQ 
Sbjct: 1   MQRLFAARTAAYASIFRKQRHSASFSSALLFDDTQLQFKESVKQFAQDNIAPHAAKIDQM 60

Query: 61  NSFPQDVNLWKLMGDFNLHGITAPEEYGGLGLGYLYHCMAMEEISRASGSVALSFGAHSN 120
           NSFPQDVNLWKLMGDFNLHGITAPEEYGGLGLGYLYHC+AMEEISRASGSVALSFGAHSN
Sbjct: 61  NSFPQDVNLWKLMGDFNLHGITAPEEYGGLGLGYLYHCIAMEEISRASGSVALSFGAHSN 120

Query: 121 LCINQLVRNGNPAQKEKYLPKLIRGEHVGALAMSEPNAGSDVVSMKCKADRVDGGYVING 180
           LCINQLVRNG+PAQKEKYLPKLI+GEHVGALAMSEPNAGSDVVSMKCKADRVDGGY+ING
Sbjct: 121 LCINQLVRNGSPAQKEKYLPKLIKGEHVGALAMSEPNAGSDVVSMKCKADRVDGGYIING 180

Query: 181 NKMWCTNGPTAQTLVVYAKTDANAGSKGITAFIIEKGMPGFSTAQKLDKLGMRGSDTCEL 240
           NKMWCTNGPTAQTLVVYAKTDANAGSKGITAFIIEKGMPGFSTAQKLDKLGMRGSDTCEL
Sbjct: 181 NKMWCTNGPTAQTLVVYAKTDANAGSKGITAFIIEKGMPGFSTAQKLDKLGMRGSDTCEL 240

Query: 241 VFENCFVPEENILGQEGKGVYVMMSGLDLERLVLAAGPLGLMQACLDVVLPYVKQREQFG 300
           VFENCFVPEEN+LGQEGKGVYVMMSGLDLERLVLAAGPLGLMQACLDVVLPYVKQREQFG
Sbjct: 241 VFENCFVPEENVLGQEGKGVYVMMSGLDLERLVLAAGPLGLMQACLDVVLPYVKQREQFG 300

Query: 301 RPIGEFQFIQGKLADMYTSLQSSRAYVYSVARDCDSGKVDPKDCAGVILCAAERATQVAL 360
           RPIGEFQFIQGKLADMYTSLQSSR+YVYSVARDCDSGKVDPKDCAGVILCAAERATQVAL
Sbjct: 301 RPIGEFQFIQGKLADMYTSLQSSRSYVYSVARDCDSGKVDPKDCAGVILCAAERATQVAL 360

Query: 361 QAIQCLGGNGYVNEYVTGRLLRDAKLYEIGAGTSEVRRMIIGRSLFKEQ 409
           QAIQCLGGNGYVNEY TGRLLRDAKLYEIGAGTSEVRRMIIGRSLFKEQ
Sbjct: 361 QAIQCLGGNGYVNEYTTGRLLRDAKLYEIGAGTSEVRRMIIGRSLFKEQ 409

BLAST of Lsi10G012850 vs. TAIR 10
Match: AT3G45300.1 (isovaleryl-CoA-dehydrogenase )

HSP 1 Score: 724.5 bits (1869), Expect = 4.6e-209
Identity = 349/409 (85.33%), Postives = 385/409 (94.13%), Query Frame = 0

Query: 1   MQKLFAARTACA-SILRRQRHSASFSSALLFDDTQLQFKESVKQFAQDNIAPHAAKIDQT 60
           MQ+ F+AR+    ++  R+R  +S SS+LLFDDTQLQFKESV +FAQDNIAPHA +ID+T
Sbjct: 1   MQRFFSARSILGYAVKTRRRSFSSRSSSLLFDDTQLQFKESVSKFAQDNIAPHAERIDKT 60

Query: 61  NSFPQDVNLWKLMGDFNLHGITAPEEYGGLGLGYLYHCMAMEEISRASGSVALSFGAHSN 120
           NSFP+DVNLWKLMG+FNLHGITAPEEYGGLGLGYLYHC+AMEEISRASGSVALS+GAHSN
Sbjct: 61  NSFPKDVNLWKLMGEFNLHGITAPEEYGGLGLGYLYHCIAMEEISRASGSVALSYGAHSN 120

Query: 121 LCINQLVRNGNPAQKEKYLPKLIRGEHVGALAMSEPNAGSDVVSMKCKADRVDGGYVING 180
           LCINQLVRNG  AQKEKYLPKLI GEHVGALAMSEPNAGSDVV MKCKA++VDGGY++NG
Sbjct: 121 LCINQLVRNGTAAQKEKYLPKLISGEHVGALAMSEPNAGSDVVGMKCKAEKVDGGYILNG 180

Query: 181 NKMWCTNGPTAQTLVVYAKTDANAGSKGITAFIIEKGMPGFSTAQKLDKLGMRGSDTCEL 240
           NKMWCTNGP+A+TLVVYAKTD  AGSKGITAFIIEKGM GFSTAQKLDKLGMRGSDTCEL
Sbjct: 181 NKMWCTNGPSAETLVVYAKTDTKAGSKGITAFIIEKGMTGFSTAQKLDKLGMRGSDTCEL 240

Query: 241 VFENCFVPEENILGQEGKGVYVMMSGLDLERLVLAAGPLGLMQACLDVVLPYVKQREQFG 300
           VFENCFVPEENIL +EGKGVYV+MSGLDLERLVLAAGPLG+MQACLD VLPY++QREQFG
Sbjct: 241 VFENCFVPEENILDKEGKGVYVLMSGLDLERLVLAAGPLGIMQACLDNVLPYIRQREQFG 300

Query: 301 RPIGEFQFIQGKLADMYTSLQSSRAYVYSVARDCDSGKVDPKDCAGVILCAAERATQVAL 360
           RP+GEFQFIQGK+ADMYT+LQSSR+YVYSVARDCD+GKVDPKDCAG ILCAAERATQVAL
Sbjct: 301 RPVGEFQFIQGKVADMYTALQSSRSYVYSVARDCDNGKVDPKDCAGTILCAAERATQVAL 360

Query: 361 QAIQCLGGNGYVNEYVTGRLLRDAKLYEIGAGTSEVRRMIIGRSLFKEQ 409
           QAIQCLGGNGY+NEY TGRLLRDAKLYEIGAGTSE+RR++IGR LFKE+
Sbjct: 361 QAIQCLGGNGYINEYATGRLLRDAKLYEIGAGTSEIRRIVIGRELFKEE 409

BLAST of Lsi10G012850 vs. TAIR 10
Match: AT3G51840.1 (acyl-CoA oxidase 4 )

HSP 1 Score: 160.6 bits (405), Expect = 2.6e-39
Identity = 101/368 (27.45%), Postives = 186/368 (50.54%), Query Frame = 0

Query: 38  KESVKQFAQDNIAPHAAKIDQTNSFPQDVNLWKLMGDFNLHGITAPEEYGGLGLGYLYHC 97
           ++ V++  +  +AP   +  +   FP   ++   +G   + G  + + YG  GL    + 
Sbjct: 63  RKKVRECMEKEVAPIMTEYWEKAEFP--FHITPKLGAMGVAG-GSIKGYGCPGLSITANA 122

Query: 98  MAMEEISRASGSVALSFGAHSNLCINQLVRNGNPAQKEKYLPKLIRGEHVGALAMSEPNA 157
           +A  EI+R   S +     HS+L +  +   G+ AQKEKYLP L +   V   A++EP+ 
Sbjct: 123 IATAEIARVDASCSTFILVHSSLGMLTIALCGSEAQKEKYLPSLAQLNTVACWALTEPDN 182

Query: 158 GSDVVSMKCKADRVDGGYVINGNKMWCTNGPTAQTLVVYAKTDANAGSKGITAFIIEKGM 217
           GSD   +   A +V+GG+ ING K W  N   A  L+++A+   N  +  I  FI++K  
Sbjct: 183 GSDASGLGTTATKVEGGWKINGQKRWIGNSTFADLLIIFAR---NTTTNQINGFIVKKDA 242

Query: 218 PGFSTAQKLDKLGMRGSDTCELVFENCFVPEENILGQEGKGVYVMMSG-LDLERLVLAAG 277
           PG    +  +K+G+R     +++ +N FVP+E+ L   G   +   S  L + R+++A  
Sbjct: 243 PGLKATKIPNKIGLRMVQNGDILLQNVFVPDEDRL--PGVNSFQDTSKVLAVSRVMVAWQ 302

Query: 278 PLGLMQACLDVVLPYVKQREQFGRPIGEFQFIQGKLADMYTSLQSSRAYVYSVARDCDSG 337
           P+G+     D+   Y+K+R+QFG P+  FQ  Q KL  M  ++Q+     + + +  ++G
Sbjct: 303 PIGISMGIYDMCHRYLKERKQFGAPLAAFQLNQQKLVQMLGNVQAMFLMGWRLCKLYETG 362

Query: 338 KVDPKDCAGVILCAAERATQVALQAIQCLGGNGYVNEYVTGRLLRDAKLYEIGAGTSEVR 397
           ++ P   +      + +A + A    + LGGNG + +++  +   D +      GT ++ 
Sbjct: 363 QMTPGQASLGKAWISSKARETASLGRELLGGNGILADFLVAKAFCDLEPIYTYEGTYDIN 422

Query: 398 RMIIGRSL 405
            ++ GR +
Sbjct: 423 TLVTGREV 422

BLAST of Lsi10G012850 vs. TAIR 10
Match: AT3G06810.1 (acyl-CoA dehydrogenase-related )

HSP 1 Score: 110.2 bits (274), Expect = 4.1e-24
Identity = 98/339 (28.91%), Postives = 146/339 (43.07%), Query Frame = 0

Query: 76  NLHGITAPEEYG-GL-GLGYLYHCMAMEEISRASGSVALSFGAHSNLCINQLVRNGNPAQ 135
           NL G +  + +G GL  L Y Y C  M     A      + GA     +  ++R GN  Q
Sbjct: 481 NLSGKSFDQLFGEGLTNLEYGYLCEIMGRSVWA--PQVFNCGAPDTGNMEVILRYGNKEQ 540

Query: 136 KEKYLPKLIRGEHVGALAMSEPN-AGSDVVSMKCKADRVDGGYVINGNKMWCTNG--PTA 195
             ++L  L+ G      AM+EP  A SD  +++C   R    YVING K W +    P  
Sbjct: 541 ISEWLIPLLEGRIRSGFAMTEPQVASSDATNIECSIRRQGDSYVINGTKWWTSGAMDPRC 600

Query: 196 QTLVVYAKTDANAGS-KGITAFIIEKGMPGFSTAQKLDKLGMRGS--DTCELVFENCFVP 255
           + L++  KTD NA   K  +  +++   PG S  + L   G   +     E+ FEN  VP
Sbjct: 601 RVLILMGKTDFNAPKHKQQSMILVDMRTPGISVKRPLTVFGFDDAPHGHAEISFENVVVP 660

Query: 256 EENILGQEGKGVYVMMSGLDLERLVLAAGPLGLMQACLDVVLPYVKQREQFGRPIGEFQF 315
            +NIL  EG+G  +    L   RL      +G  +  ++++      R+ FG+ I +   
Sbjct: 661 AKNILLGEGRGFEIAQGRLGPGRLHHCMRLIGAAERGMELMAQRALSRKTFGKFIAQHGS 720

Query: 316 IQGKLADMYTSLQSSRAYVYSVARDCDSGKVDPKDCAGVILCAAERATQVALQ----AIQ 375
               LA +   L+ +R  V   A   D  K   K   G++  A   A  +AL+    AIQ
Sbjct: 721 FVSDLAKLRVELEGTRLLVLEAADHLD--KFGNKKARGILAMAKVAAPNMALKVLDTAIQ 780

Query: 376 CLGGNGYVNEYVTGRLLRDAKLYEIGAGTSEVRRMIIGR 403
             G  G  ++ V   L   A+   I  G  EV    IG+
Sbjct: 781 VHGAAGVSSDTVLAHLWATARTLRIADGPDEVHLGTIGK 815

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SWG06.5e-20885.33Isovaleryl-CoA dehydrogenase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=I... [more]
Q9FS877.4e-20484.22Isovaleryl-CoA dehydrogenase, mitochondrial OS=Solanum tuberosum OX=4113 GN=IVD ... [more]
Q75IM99.0e-20285.01Isovaleryl-CoA dehydrogenase, mitochondrial OS=Oryza sativa subsp. japonica OX=3... [more]
Q9FS881.1e-19980.102-methylacyl-CoA dehydrogenase, mitochondrial OS=Solanum tuberosum OX=4113 GN=2M... [more]
Q9JHI53.0e-14968.53Isovaleryl-CoA dehydrogenase, mitochondrial OS=Mus musculus OX=10090 GN=Ivd PE=1... [more]
Match NameE-valueIdentityDescription
A0A5D3CRA13.7e-23097.30Isovaleryl-CoA dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3CKE13.7e-23097.30isovaleryl-CoA dehydrogenase, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103501... [more]
A0A6J1CIC16.9e-22996.81isovaleryl-CoA dehydrogenase, mitochondrial OS=Momordica charantia OX=3673 GN=LO... [more]
A0A6J1H1Y47.6e-22896.82isovaleryl-CoA dehydrogenase, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1K1P81.9e-22696.58isovaleryl-CoA dehydrogenase, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
Match NameE-valueIdentityDescription
XP_038901894.13.6e-23298.77isovaleryl-CoA dehydrogenase, mitochondrial [Benincasa hispida][more]
XP_008463922.17.5e-23097.30PREDICTED: isovaleryl-CoA dehydrogenase, mitochondrial [Cucumis melo] >KAA003532... [more]
XP_022140593.11.4e-22896.81isovaleryl-CoA dehydrogenase, mitochondrial [Momordica charantia][more]
XP_011657216.17.1e-22896.57isovaleryl-CoA dehydrogenase, mitochondrial [Cucumis sativus] >KAE8647055.1 hypo... [more]
XP_022957905.11.6e-22796.82isovaleryl-CoA dehydrogenase, mitochondrial [Cucurbita moschata] >KAG7013907.1 I... [more]
Match NameE-valueIdentityDescription
AT3G45300.14.6e-20985.33isovaleryl-CoA-dehydrogenase [more]
AT3G51840.12.6e-3927.45acyl-CoA oxidase 4 [more]
AT3G06810.14.1e-2428.91acyl-CoA dehydrogenase-related [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePIRSFPIRSF016578PIGMcoord: 155..408
e-value: 1.1E-17
score: 60.9
coord: 21..122
e-value: 0.036
score: 9.8
NoneNo IPR availableGENE3D1.20.140.10coord: 261..404
e-value: 4.7E-49
score: 168.1
NoneNo IPR availableGENE3D2.40.110.10coord: 148..260
e-value: 2.8E-45
score: 155.1
NoneNo IPR availablePANTHERPTHR43884ACYL-COA DEHYDROGENASEcoord: 1..408
NoneNo IPR availablePANTHERPTHR43884:SF272-METHYLACYL-COA DEHYDROGENASE, MITOCHONDRIALcoord: 1..408
IPR009075Acyl-CoA dehydrogenase/oxidase C-terminalPFAMPF00441Acyl-CoA_dh_1coord: 256..404
e-value: 1.1E-43
score: 149.0
IPR013786Acyl-CoA dehydrogenase/oxidase, N-terminalPFAMPF02771Acyl-CoA_dh_Ncoord: 32..145
e-value: 6.9E-35
score: 120.1
IPR037069Acyl-CoA dehydrogenase/oxidase, N-terminal domain superfamilyGENE3D1.10.540.10coord: 14..146
e-value: 7.3E-37
score: 128.2
IPR006091Acyl-CoA oxidase/dehydrogenase, central domainPFAMPF02770Acyl-CoA_dh_Mcoord: 149..244
e-value: 1.4E-26
score: 92.5
IPR006089Acyl-CoA dehydrogenase, conserved sitePROSITEPS00073ACYL_COA_DH_2coord: 363..382
IPR006089Acyl-CoA dehydrogenase, conserved sitePROSITEPS00072ACYL_COA_DH_1coord: 151..163
IPR034183Isovaleryl-CoA dehydrogenaseCDDcd01156IVDcoord: 30..406
e-value: 0.0
score: 720.735
IPR036250Acyl-CoA dehydrogenase-like, C-terminalSUPERFAMILY47203Acyl-CoA dehydrogenase C-terminal domain-likecoord: 254..406
IPR009100Acyl-CoA dehydrogenase/oxidase, N-terminal and middle domain superfamilySUPERFAMILY56645Acyl-CoA dehydrogenase NM domain-likecoord: 27..267

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi10G012850.1Lsi10G012850.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006552 leucine catabolic process
cellular_component GO:0005759 mitochondrial matrix
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0008470 isovaleryl-CoA dehydrogenase activity
molecular_function GO:0003995 acyl-CoA dehydrogenase activity
molecular_function GO:0016627 oxidoreductase activity, acting on the CH-CH group of donors