Lsi10G009800 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi10G009800
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptiontransmembrane protein 45A
Locationchr10: 14147541 .. 14149739 (-)
RNA-Seq ExpressionLsi10G009800
SyntenyLsi10G009800
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCATGTAATAGCAAGATATATAATTTATATATTCGTCAAAGTTCAATAGCCTTCTTTTCTTTTTTAAAAGTTAAACAATTCATACAAGTATTCGATTCTCTTACCTTTACTCAATTAAATATATTGTTTCACTATTACAATAGTATCGAGTCAAATACATTGTAGTAAATAATGTCACCTATAAATGATCATATTTGAGATCAAAATTTCAATTTTTATTATATTCAAATATAGCTCACAACAACTATAATGTTAATCAATTTCATTTCAATTTTCAACGATAATGAAGTTTATAAATTTCATTTTCCACAATCCAAAAAATAAATGTTGAAAAAATTGGCAAAAAAAGAAGAGAGACAATCTCTTGGAAAGAAAGAAACTTTATTATGAATGTACAAGGATGGACACTTAGAGAGAATACTCTTTTACACTCTCTAACTCACTCACTTTTCTTCCCTAACTCTCTAGCTTTTTGCTTTTTCTTCCTCTCTTTGCTTGCTTTACAAATGGGGTCACACCTCCTATTTATAGGTGTGAGATCCTTGGAGCTTAAGCTAGGAGATTTTTCTAGACTTCTCTTAGATCTAAACTATTCTAGACATTTTTATGACATTATTCCTATCTAAAGGAATTTTCTAGAATTTCGAAAGCTTAGGATATTTCTAGAACATTTCTTAATGAGATACTTATTTACATACTTCTAGATAAATCTAGAAATGTCTAATATTTACACTAAATTATATAAATATCTCAAGAAGCTTCTAGAATAGTTAGTGTTGCTTCTATTTCAACAATAAATATTATTTATATCAATCACATTTACCAAATATGTTTTTGAAAAATATACTTCTACAAGATCAAAATCAAAAGCTAGATTACCAACGGCAGGCTAGCTAGCTGTGAGATGAGGGGTATTTTTATGTCTTTTGAATATTATTGCAATTATAACGATATTATGACAACAGAAATTATTGTTATACATCTACTAATAGGGGTTTAATTAAGCTATGGTAATTACTGTTTCCTTGCTGTTGGTTAGGTAATTATTTTGTATCATATTTTTCTTCTGTTGGGTTGAGAAAGGCAGAGACAGAGATGCAAAATTGTAATTGACGACAAATAATTAAAAATTAGGCCGCCTTTTTTTGTTAAAAGATTAGGACTTGAAGACTTGCTTCACACCAAACAAGCCAAGAGACTGCGAGCTTGGGTTGGCCCACTAATCCTTCTACTACACCTAATCAAATACGTAACAATAATATTTGGTCAACTCTAAACTATCTCTCATTTTATTTTTTTTTTCTTCTTCAAAGCTTCAACAAAATCATCCCCCAACTCAATTCGAATCCTCTAGACCCTTTGACCCTTCTCTTATAAGTTACATTACATAGTCTTAAAATTCCCTTCATAACTCTAACTCCCTTCTCCTCTCCCGGCCTCTAATTTAAAGTTAGTTCTCGTTTTCATTTTTCTTTTATAAAGGGTAGTGATTAATATTATTATGGGGACTTTAGTGGGTCATGTAGCACCCGGGTTTGGGTTCTTCATCATTGGCCTATGGCATCTCTATAACAACATAAGGATTGAGGCTTGTAAGGCTCGTAACGACGACTCTTCAAATACCGCCATATTTCTCTCGTGGTTCCCCACTTCAAGATTCAAGTACTTAGAGCCGTTGTTGATCATGGTTGGTGCCTCGGCCTCTATAGCGATGGAGCTCTTCATCGGCCCGCAAAGGCACCAACCGTTCGACACCGACGGAACCATCCCTTCCTACCATCTCCACAACTTCGAACACTCCAACATTTCTATGAGTTTCTTTGTGTTCGGTGCGTTTTGTGTTATCCTGGATCGGCTTCGTCCGAAAGCTTATTTTGAGCTGACACAGTTTGTTGGGGGTGTGGCTTTTGGTCAAGAGCTTCTATTGTTTCATCTCCACTCTGCTGACCATATGGGCCCTGAAGGACAATACCACTTGCTTCTTCAGCTTCTTGTGCTTGTCTCTTTTGCCACTACTCTTCTTGGGATTGCTCTTCCCACTAGCTTCTTGGTGGCGTTTGTGAGATCTCTTAGTATTCTTTTTCAGGGCCTCTGGCTTATGCTTATGGGCTTCGCGCTTTGGACTCCTTCTTTCATTTCCAAAGGTACCTCACCCTCACTTCTAATAAAAAATTATGATCAACGTCATAAATTGTAA

mRNA sequence

ATGCATCTTCAACAAAATCATCCCCCAACTCAATTCGAATCCTCTAGACCCTTTGACCCTTCTCTTATAATGATTAATATTATTATGGGGACTTTAGTGGGTCATGTAGCACCCGGGTTTGGGTTCTTCATCATTGGCCTATGGCATCTCTATAACAACATAAGGATTGAGGCTTGTAAGGCTCGTAACGACGACTCTTCAAATACCGCCATATTTCTCTCGTGGTTCCCCACTTCAAGATTCAAGTACTTAGAGCCGTTGTTGATCATGGTTGGTGCCTCGGCCTCTATAGCGATGGAGCTCTTCATCGGCCCGCAAAGGCACCAACCGTTCGACACCGACGGAACCATCCCTTCCTACCATCTCCACAACTTCGAACACTCCAACATTTCTATGAGTTTCTTTGTGTTCGGTGCGTTTTGTGTTATCCTGGATCGGCTTCGTCCGAAAGCTTATTTTGAGCTGACACAGTTTGTTGGGGGTGTGGCTTTTGGTCAAGAGCTTCTATTGTTTCATCTCCACTCTGCTGACCATATGGGCCCTGAAGGACAATACCACTTGCTTCTTCAGCTTCTTGTGCTTGTCTCTTTTGCCACTACTCTTCTTGGGATTGCTCTTCCCACTAGCTTCTTGGTGGCGTTTGTGAGATCTCTTAGTATTCTTTTTCAGGGCCTCTGGCTTATGCTTATGGGCTTCGCGCTTTGGACTCCTTCTTTCATTTCCAAAGGTACCTCACCCTCACTTCTAATAAAAAATTATGATCAACGTCATAAATTGTAA

Coding sequence (CDS)

ATGCATCTTCAACAAAATCATCCCCCAACTCAATTCGAATCCTCTAGACCCTTTGACCCTTCTCTTATAATGATTAATATTATTATGGGGACTTTAGTGGGTCATGTAGCACCCGGGTTTGGGTTCTTCATCATTGGCCTATGGCATCTCTATAACAACATAAGGATTGAGGCTTGTAAGGCTCGTAACGACGACTCTTCAAATACCGCCATATTTCTCTCGTGGTTCCCCACTTCAAGATTCAAGTACTTAGAGCCGTTGTTGATCATGGTTGGTGCCTCGGCCTCTATAGCGATGGAGCTCTTCATCGGCCCGCAAAGGCACCAACCGTTCGACACCGACGGAACCATCCCTTCCTACCATCTCCACAACTTCGAACACTCCAACATTTCTATGAGTTTCTTTGTGTTCGGTGCGTTTTGTGTTATCCTGGATCGGCTTCGTCCGAAAGCTTATTTTGAGCTGACACAGTTTGTTGGGGGTGTGGCTTTTGGTCAAGAGCTTCTATTGTTTCATCTCCACTCTGCTGACCATATGGGCCCTGAAGGACAATACCACTTGCTTCTTCAGCTTCTTGTGCTTGTCTCTTTTGCCACTACTCTTCTTGGGATTGCTCTTCCCACTAGCTTCTTGGTGGCGTTTGTGAGATCTCTTAGTATTCTTTTTCAGGGCCTCTGGCTTATGCTTATGGGCTTCGCGCTTTGGACTCCTTCTTTCATTTCCAAAGGTACCTCACCCTCACTTCTAATAAAAAATTATGATCAACGTCATAAATTGTAA

Protein sequence

MHLQQNHPPTQFESSRPFDPSLIMINIIMGTLVGHVAPGFGFFIIGLWHLYNNIRIEACKARNDDSSNTAIFLSWFPTSRFKYLEPLLIMVGASASIAMELFIGPQRHQPFDTDGTIPSYHLHNFEHSNISMSFFVFGAFCVILDRLRPKAYFELTQFVGGVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVLVSFATTLLGIALPTSFLVAFVRSLSILFQGLWLMLMGFALWTPSFISKGTSPSLLIKNYDQRHKL
Homology
BLAST of Lsi10G009800 vs. ExPASy Swiss-Prot
Match: Q9NWC5 (Transmembrane protein 45A OS=Homo sapiens OX=9606 GN=TMEM45A PE=1 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 2.4e-06
Identity = 59/213 (27.70%), Postives = 84/213 (39.44%), Query Frame = 0

Query: 29  MGTLVGHVAPGFGFFIIGLWHLYNNIRIEACKARNDDSSNTAIFLSWFPTSRFKYLEPLL 88
           MG   GH  PG  FFIIGLW    +I    CK +      T    S     R + LE + 
Sbjct: 1   MGNFRGHALPGTFFFIIGLWWCTKSILKYICKKQ----KRTCYLGSKTLFYRLEILEGIT 60

Query: 89  IMVGASASIAMELFIGPQRHQPFDTDGTIPSYHLHNFEHSNISMSFFVFGA---FCVILD 148
           I+  A   +A E FI    H             L  + H  +   F + G     C  + 
Sbjct: 61  IVGMALTGMAGEQFIPGGPHLMLYDYKQGHWNQLLGWHHFTMYFFFGLLGVADILCFTIS 120

Query: 149 RLRPKAYFELTQFVGGVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVLVSFATTLLGIA 208
            L       LT+ +   A   E  +F+ H+      +   H LL L+V ++     L   
Sbjct: 121 SLP----VSLTKLMLSNALFVEAFIFYNHTHGREMLDIFVHQLLVLVVFLTGLVAFLEFL 180

Query: 209 LPTSFLVAFVRSLSILFQGLWLMLMGFALWTPS 239
           +  + L+  +RS  IL QG W   +GF L+ PS
Sbjct: 181 VRNNVLLELLRSSLILLQGSWFFQIGFVLYPPS 205

BLAST of Lsi10G009800 vs. ExPASy TrEMBL
Match: A0A1S3BMV5 (transmembrane protein 45A OS=Cucumis melo OX=3656 GN=LOC103491576 PE=3 SV=1)

HSP 1 Score: 403.3 bits (1035), Expect = 7.7e-109
Identity = 196/215 (91.16%), Postives = 205/215 (95.35%), Query Frame = 0

Query: 29  MGTLVGHVAPGFGFFIIGLWHLYNNIRIEACKARNDDSSNTAIFLSWFPTSRFKYLEPLL 88
           MGTLVGHVAPGFGFFIIGLWHL+NNI+IEAC   N++  NTAIFLSWFPTSRFKYLEP L
Sbjct: 1   MGTLVGHVAPGFGFFIIGLWHLFNNIKIEACNNNNNNHPNTAIFLSWFPTSRFKYLEPFL 60

Query: 89  IMVGASASIAMELFIGPQRHQPFDTDGTIPSYHLHNFEHSNISMSFFVFGAFCVILDRLR 148
           IMVGASASIAMELFIGPQRHQPFDTDGTIPSYHLHNFEHSNISM+FFVF AFCV+LDR+R
Sbjct: 61  IMVGASASIAMELFIGPQRHQPFDTDGTIPSYHLHNFEHSNISMTFFVFAAFCVVLDRVR 120

Query: 149 PKAYFELTQFVGGVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVLVSFATTLLGIALPT 208
           PKAYFELTQFVG VAFGQELLLFHLHSADHMGPEGQYHLLLQLLVLVSFATTLLGIALP 
Sbjct: 121 PKAYFELTQFVGTVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVLVSFATTLLGIALPN 180

Query: 209 SFLVAFVRSLSILFQGLWLMLMGFALWTPSFISKG 244
           SFLVAFVRSLSI+FQGLWL+LMGFALWTPSFI KG
Sbjct: 181 SFLVAFVRSLSIVFQGLWLILMGFALWTPSFIPKG 215

BLAST of Lsi10G009800 vs. ExPASy TrEMBL
Match: A0A0A0KX38 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G280600 PE=3 SV=1)

HSP 1 Score: 392.1 bits (1006), Expect = 1.8e-105
Identity = 192/215 (89.30%), Postives = 204/215 (94.88%), Query Frame = 0

Query: 29  MGTLVGHVAPGFGFFIIGLWHLYNNIRIEACKARNDDSSNTAIFLSWFPTSRFKYLEPLL 88
           MGTLVGHVAPGFGFFIIGLWHL+NNI+IEAC   N++  NTAIFLSWFPTSRFKYLEP L
Sbjct: 1   MGTLVGHVAPGFGFFIIGLWHLFNNIKIEAC---NNNPPNTAIFLSWFPTSRFKYLEPFL 60

Query: 89  IMVGASASIAMELFIGPQRHQPFDTDGTIPSYHLHNFEHSNISMSFFVFGAFCVILDRLR 148
           IMVGASASIAMELFIGPQRHQPFD DGTIPSYHLHNFEHSNISM+FFVF AFCV+LD++R
Sbjct: 61  IMVGASASIAMELFIGPQRHQPFDIDGTIPSYHLHNFEHSNISMTFFVFAAFCVVLDQVR 120

Query: 149 PKAYFELTQFVGGVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVLVSFATTLLGIALPT 208
           PKAYFELTQFVG VAFGQELLLFHLHSADHMGPEGQYHLLLQLLV+VSFATTLLGIALP 
Sbjct: 121 PKAYFELTQFVGTVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVVVSFATTLLGIALPN 180

Query: 209 SFLVAFVRSLSILFQGLWLMLMGFALWTPSFISKG 244
           SFL+AFVRSLSI+FQGLWL+LMGFALWTPSFI KG
Sbjct: 181 SFLLAFVRSLSIVFQGLWLILMGFALWTPSFIPKG 212

BLAST of Lsi10G009800 vs. ExPASy TrEMBL
Match: A0A6J1FXU7 (transmembrane protein 45A-like OS=Cucurbita moschata OX=3662 GN=LOC111448740 PE=3 SV=1)

HSP 1 Score: 369.4 bits (947), Expect = 1.2e-98
Identity = 182/215 (84.65%), Postives = 195/215 (90.70%), Query Frame = 0

Query: 29  MGTLVGHVAPGFGFFIIGLWHLYNNIRIEACKARNDDSSNTAIFLSWFPTSRFKYLEPLL 88
           MGTLVGHVAPGFGFFIIGLWHL+NNI+IEA      +  N+ IFLSWFPTSRFKYLEPLL
Sbjct: 1   MGTLVGHVAPGFGFFIIGLWHLFNNIKIEA----RTNPPNSPIFLSWFPTSRFKYLEPLL 60

Query: 89  IMVGASASIAMELFIGPQRHQPFDTDGTIPSYHLHNFEHSNISMSFFVFGAFCVILDRLR 148
           IM+GASAS+AMELFIGPQRH PFDTDGTIPS HLHN EHSNISM+FFVF AF VILDRLR
Sbjct: 61  IMLGASASVAMELFIGPQRHHPFDTDGTIPSNHLHNIEHSNISMTFFVFAAFSVILDRLR 120

Query: 149 PKAYFELTQFVGGVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVLVSFATTLLGIALPT 208
           PKAYFELTQF+G VAFGQELLLFHLHSADH GPEGQYH+LLQLLVL+S ATTLLGIALPT
Sbjct: 121 PKAYFELTQFLGAVAFGQELLLFHLHSADHNGPEGQYHMLLQLLVLLSLATTLLGIALPT 180

Query: 209 SFLVAFVRSLSILFQGLWLMLMGFALWTPSFISKG 244
           SFLVAF+RS SI+FQG+WLMLMGF LWTPS ISKG
Sbjct: 181 SFLVAFIRSFSIIFQGVWLMLMGFTLWTPSLISKG 211

BLAST of Lsi10G009800 vs. ExPASy TrEMBL
Match: A0A6J1JGR5 (transmembrane protein 45A-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111484363 PE=3 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 3.6e-98
Identity = 180/215 (83.72%), Postives = 194/215 (90.23%), Query Frame = 0

Query: 29  MGTLVGHVAPGFGFFIIGLWHLYNNIRIEACKARNDDSSNTAIFLSWFPTSRFKYLEPLL 88
           MGTLVGHVAPGFGFFIIGLWHL+NN++IEA         N+ +FLSWFPTSRFKYLEPLL
Sbjct: 1   MGTLVGHVAPGFGFFIIGLWHLFNNMKIEA----RSSPPNSPVFLSWFPTSRFKYLEPLL 60

Query: 89  IMVGASASIAMELFIGPQRHQPFDTDGTIPSYHLHNFEHSNISMSFFVFGAFCVILDRLR 148
           IM+GASAS+AMELFIGPQRH PFDTDGTIPS HLHN EHSNISM+FFVF AF VILDRLR
Sbjct: 61  IMLGASASVAMELFIGPQRHHPFDTDGTIPSNHLHNIEHSNISMTFFVFAAFSVILDRLR 120

Query: 149 PKAYFELTQFVGGVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVLVSFATTLLGIALPT 208
           PKAYFELTQF+G VAFGQELLLFHLHSADH GPEGQYH+LLQLLVL+S ATTLLGIALPT
Sbjct: 121 PKAYFELTQFLGAVAFGQELLLFHLHSADHNGPEGQYHMLLQLLVLLSLATTLLGIALPT 180

Query: 209 SFLVAFVRSLSILFQGLWLMLMGFALWTPSFISKG 244
           SFLVAF+RS SI+FQG+WLMLMGF LWTPS ISKG
Sbjct: 181 SFLVAFIRSFSIIFQGVWLMLMGFTLWTPSLISKG 211

BLAST of Lsi10G009800 vs. ExPASy TrEMBL
Match: A0A6J1JEQ6 (transmembrane protein 45A-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111484363 PE=3 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 3.6e-98
Identity = 180/215 (83.72%), Postives = 194/215 (90.23%), Query Frame = 0

Query: 29  MGTLVGHVAPGFGFFIIGLWHLYNNIRIEACKARNDDSSNTAIFLSWFPTSRFKYLEPLL 88
           MGTLVGHVAPGFGFFIIGLWHL+NN++IEA         N+ +FLSWFPTSRFKYLEPLL
Sbjct: 1   MGTLVGHVAPGFGFFIIGLWHLFNNMKIEA----RSSPPNSPVFLSWFPTSRFKYLEPLL 60

Query: 89  IMVGASASIAMELFIGPQRHQPFDTDGTIPSYHLHNFEHSNISMSFFVFGAFCVILDRLR 148
           IM+GASAS+AMELFIGPQRH PFDTDGTIPS HLHN EHSNISM+FFVF AF VILDRLR
Sbjct: 61  IMLGASASVAMELFIGPQRHHPFDTDGTIPSNHLHNIEHSNISMTFFVFAAFSVILDRLR 120

Query: 149 PKAYFELTQFVGGVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVLVSFATTLLGIALPT 208
           PKAYFELTQF+G VAFGQELLLFHLHSADH GPEGQYH+LLQLLVL+S ATTLLGIALPT
Sbjct: 121 PKAYFELTQFLGAVAFGQELLLFHLHSADHNGPEGQYHMLLQLLVLLSLATTLLGIALPT 180

Query: 209 SFLVAFVRSLSILFQGLWLMLMGFALWTPSFISKG 244
           SFLVAF+RS SI+FQG+WLMLMGF LWTPS ISKG
Sbjct: 181 SFLVAFIRSFSIIFQGVWLMLMGFTLWTPSLISKG 211

BLAST of Lsi10G009800 vs. NCBI nr
Match: XP_008449792.1 (PREDICTED: transmembrane protein 45A [Cucumis melo])

HSP 1 Score: 403.3 bits (1035), Expect = 1.6e-108
Identity = 196/215 (91.16%), Postives = 205/215 (95.35%), Query Frame = 0

Query: 29  MGTLVGHVAPGFGFFIIGLWHLYNNIRIEACKARNDDSSNTAIFLSWFPTSRFKYLEPLL 88
           MGTLVGHVAPGFGFFIIGLWHL+NNI+IEAC   N++  NTAIFLSWFPTSRFKYLEP L
Sbjct: 1   MGTLVGHVAPGFGFFIIGLWHLFNNIKIEACNNNNNNHPNTAIFLSWFPTSRFKYLEPFL 60

Query: 89  IMVGASASIAMELFIGPQRHQPFDTDGTIPSYHLHNFEHSNISMSFFVFGAFCVILDRLR 148
           IMVGASASIAMELFIGPQRHQPFDTDGTIPSYHLHNFEHSNISM+FFVF AFCV+LDR+R
Sbjct: 61  IMVGASASIAMELFIGPQRHQPFDTDGTIPSYHLHNFEHSNISMTFFVFAAFCVVLDRVR 120

Query: 149 PKAYFELTQFVGGVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVLVSFATTLLGIALPT 208
           PKAYFELTQFVG VAFGQELLLFHLHSADHMGPEGQYHLLLQLLVLVSFATTLLGIALP 
Sbjct: 121 PKAYFELTQFVGTVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVLVSFATTLLGIALPN 180

Query: 209 SFLVAFVRSLSILFQGLWLMLMGFALWTPSFISKG 244
           SFLVAFVRSLSI+FQGLWL+LMGFALWTPSFI KG
Sbjct: 181 SFLVAFVRSLSIVFQGLWLILMGFALWTPSFIPKG 215

BLAST of Lsi10G009800 vs. NCBI nr
Match: XP_038901896.1 (transmembrane protein 45B-like [Benincasa hispida])

HSP 1 Score: 395.2 bits (1014), Expect = 4.3e-106
Identity = 195/215 (90.70%), Postives = 202/215 (93.95%), Query Frame = 0

Query: 29  MGTLVGHVAPGFGFFIIGLWHLYNNIRIEACKARNDDSSNTAIFLSWFPTSRFKYLEPLL 88
           MGTLVGHVAPGFGFFIIGLWHL+NN +IE   ARN+D  NT  FLSWFPTSRFKYLEP L
Sbjct: 1   MGTLVGHVAPGFGFFIIGLWHLFNNTKIE---ARNNDPPNTVKFLSWFPTSRFKYLEPCL 60

Query: 89  IMVGASASIAMELFIGPQRHQPFDTDGTIPSYHLHNFEHSNISMSFFVFGAFCVILDRLR 148
           IMVGASASIAMELFIGPQ+HQPFDTDGTIPSYHLHNFEHSNISM+FFVF AFCV+LDRLR
Sbjct: 61  IMVGASASIAMELFIGPQQHQPFDTDGTIPSYHLHNFEHSNISMTFFVFAAFCVVLDRLR 120

Query: 149 PKAYFELTQFVGGVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVLVSFATTLLGIALPT 208
           PKAYFELTQFVGGVAFGQELLLFHLHSADHMGPEGQYHLLLQLLV VSFATTLLGIA PT
Sbjct: 121 PKAYFELTQFVGGVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVFVSFATTLLGIAFPT 180

Query: 209 SFLVAFVRSLSILFQGLWLMLMGFALWTPSFISKG 244
           SFLVAFVRSLSI+FQGLWLMLMGFALWTPSFI KG
Sbjct: 181 SFLVAFVRSLSIVFQGLWLMLMGFALWTPSFIPKG 212

BLAST of Lsi10G009800 vs. NCBI nr
Match: XP_004142158.1 (transmembrane protein 45A [Cucumis sativus] >KGN54083.1 hypothetical protein Csa_018130 [Cucumis sativus])

HSP 1 Score: 392.1 bits (1006), Expect = 3.7e-105
Identity = 192/215 (89.30%), Postives = 204/215 (94.88%), Query Frame = 0

Query: 29  MGTLVGHVAPGFGFFIIGLWHLYNNIRIEACKARNDDSSNTAIFLSWFPTSRFKYLEPLL 88
           MGTLVGHVAPGFGFFIIGLWHL+NNI+IEAC   N++  NTAIFLSWFPTSRFKYLEP L
Sbjct: 1   MGTLVGHVAPGFGFFIIGLWHLFNNIKIEAC---NNNPPNTAIFLSWFPTSRFKYLEPFL 60

Query: 89  IMVGASASIAMELFIGPQRHQPFDTDGTIPSYHLHNFEHSNISMSFFVFGAFCVILDRLR 148
           IMVGASASIAMELFIGPQRHQPFD DGTIPSYHLHNFEHSNISM+FFVF AFCV+LD++R
Sbjct: 61  IMVGASASIAMELFIGPQRHQPFDIDGTIPSYHLHNFEHSNISMTFFVFAAFCVVLDQVR 120

Query: 149 PKAYFELTQFVGGVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVLVSFATTLLGIALPT 208
           PKAYFELTQFVG VAFGQELLLFHLHSADHMGPEGQYHLLLQLLV+VSFATTLLGIALP 
Sbjct: 121 PKAYFELTQFVGTVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVVVSFATTLLGIALPN 180

Query: 209 SFLVAFVRSLSILFQGLWLMLMGFALWTPSFISKG 244
           SFL+AFVRSLSI+FQGLWL+LMGFALWTPSFI KG
Sbjct: 181 SFLLAFVRSLSIVFQGLWLILMGFALWTPSFIPKG 212

BLAST of Lsi10G009800 vs. NCBI nr
Match: KAG7010234.1 (hypothetical protein SDJN02_27026, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 370.2 bits (949), Expect = 1.5e-98
Identity = 182/215 (84.65%), Postives = 196/215 (91.16%), Query Frame = 0

Query: 29  MGTLVGHVAPGFGFFIIGLWHLYNNIRIEACKARNDDSSNTAIFLSWFPTSRFKYLEPLL 88
           MGTLVGHVAPGFGFFIIGLWHL+NNI+IEA     ++  N+ IFLSWFPTSRFKYLEPLL
Sbjct: 1   MGTLVGHVAPGFGFFIIGLWHLFNNIKIEA----RNNPPNSPIFLSWFPTSRFKYLEPLL 60

Query: 89  IMVGASASIAMELFIGPQRHQPFDTDGTIPSYHLHNFEHSNISMSFFVFGAFCVILDRLR 148
           IM+GASAS+AMELFIGPQRH PFDTDGTIPS HLHN EHSNISM+FFVF AF VILDRLR
Sbjct: 61  IMLGASASVAMELFIGPQRHHPFDTDGTIPSNHLHNIEHSNISMTFFVFAAFSVILDRLR 120

Query: 149 PKAYFELTQFVGGVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVLVSFATTLLGIALPT 208
           PKAYFELTQF+G VAFGQELLLFHLHSADH GPEGQYH+LLQLLVL+S ATTLLGIALPT
Sbjct: 121 PKAYFELTQFLGAVAFGQELLLFHLHSADHNGPEGQYHMLLQLLVLLSLATTLLGIALPT 180

Query: 209 SFLVAFVRSLSILFQGLWLMLMGFALWTPSFISKG 244
           SFLVAF+RS SI+FQG+WLMLMGF LWTPS ISKG
Sbjct: 181 SFLVAFIRSFSIIFQGVWLMLMGFTLWTPSLISKG 211

BLAST of Lsi10G009800 vs. NCBI nr
Match: XP_022944228.1 (transmembrane protein 45A-like [Cucurbita moschata])

HSP 1 Score: 369.4 bits (947), Expect = 2.6e-98
Identity = 182/215 (84.65%), Postives = 195/215 (90.70%), Query Frame = 0

Query: 29  MGTLVGHVAPGFGFFIIGLWHLYNNIRIEACKARNDDSSNTAIFLSWFPTSRFKYLEPLL 88
           MGTLVGHVAPGFGFFIIGLWHL+NNI+IEA      +  N+ IFLSWFPTSRFKYLEPLL
Sbjct: 1   MGTLVGHVAPGFGFFIIGLWHLFNNIKIEA----RTNPPNSPIFLSWFPTSRFKYLEPLL 60

Query: 89  IMVGASASIAMELFIGPQRHQPFDTDGTIPSYHLHNFEHSNISMSFFVFGAFCVILDRLR 148
           IM+GASAS+AMELFIGPQRH PFDTDGTIPS HLHN EHSNISM+FFVF AF VILDRLR
Sbjct: 61  IMLGASASVAMELFIGPQRHHPFDTDGTIPSNHLHNIEHSNISMTFFVFAAFSVILDRLR 120

Query: 149 PKAYFELTQFVGGVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVLVSFATTLLGIALPT 208
           PKAYFELTQF+G VAFGQELLLFHLHSADH GPEGQYH+LLQLLVL+S ATTLLGIALPT
Sbjct: 121 PKAYFELTQFLGAVAFGQELLLFHLHSADHNGPEGQYHMLLQLLVLLSLATTLLGIALPT 180

Query: 209 SFLVAFVRSLSILFQGLWLMLMGFALWTPSFISKG 244
           SFLVAF+RS SI+FQG+WLMLMGF LWTPS ISKG
Sbjct: 181 SFLVAFIRSFSIIFQGVWLMLMGFTLWTPSLISKG 211

BLAST of Lsi10G009800 vs. TAIR 10
Match: AT1G55230.1 (Family of unknown function (DUF716) )

HSP 1 Score: 269.2 bits (687), Expect = 3.3e-72
Identity = 135/217 (62.21%), Postives = 163/217 (75.12%), Query Frame = 0

Query: 29  MGTLVGHVAPGFGFFIIGLWHLYNNIRIEACKARNDDSSNTAIFLSWFPTSRFKYLEPLL 88
           MGTLVGHVAPGF FF +GLWHL+NNI++         + NT     WFPTS+ ++LE  +
Sbjct: 1   MGTLVGHVAPGFAFFALGLWHLFNNIKLFCL------NPNTFTSSPWFPTSKLRHLELYI 60

Query: 89  IMVGASASIAMELFIGPQRHQPFDTDGTIPSYHLHNFEHSNISMSFFVFGAFCVILDRLR 148
           IMV +S SIA ELFIGP++HQPFD+DGTIPS HLHNFEHS+ISMSF V+  F V+LD+ R
Sbjct: 61  IMVCSSISIASELFIGPKKHQPFDSDGTIPSNHLHNFEHSSISMSFLVYAIFAVVLDQER 120

Query: 149 PKAYFE--LTQFVGGVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVLVSFATTLLGIAL 208
            ++     LT  V   AF Q+L LFHLHSADHMG EGQYH LLQL++ VSF TTL+GI L
Sbjct: 121 RRSNISHGLTMLVASSAFAQQLFLFHLHSADHMGIEGQYHKLLQLIIFVSFLTTLIGITL 180

Query: 209 PTSFLVAFVRSLSILFQGLWLMLMGFALWTPSFISKG 244
           P SFLV+FVRS SI FQG+W ++MG+ LWTPS I KG
Sbjct: 181 PKSFLVSFVRSSSITFQGVWFVVMGYMLWTPSLIPKG 211

BLAST of Lsi10G009800 vs. TAIR 10
Match: AT1G55240.1 (Family of unknown function (DUF716) )

HSP 1 Score: 251.1 bits (640), Expect = 9.4e-67
Identity = 125/217 (57.60%), Postives = 159/217 (73.27%), Query Frame = 0

Query: 29  MGTLVGHVAPGFGFFIIGLWHLYNNIRIEACKARNDDSSNTAIFLSWFPTSRFKYLEPLL 88
           MG+ +GHV PGF F  +GLWHL+NNI++  C   N  SS+T     WFP S+ ++LE  L
Sbjct: 1   MGSFLGHVLPGFAFVALGLWHLFNNIKL-FCLHPNTFSSST-----WFPISKLRHLELYL 60

Query: 89  IMVGASASIAMELFIGPQRHQPFDTDGTIPSYHLHNFEHSNISMSFFVFGAFCVILDRLR 148
           IM+ ++ASI+MELFIGP+RH PFD DGTIPS HLHNFEHS+ISMSF V+    ++LDR R
Sbjct: 61  IMLSSTASISMELFIGPRRHHPFDVDGTIPSNHLHNFEHSSISMSFLVYAVLALVLDRAR 120

Query: 149 PKAYFE--LTQFVGGVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVLVSFATTLLGIAL 208
           P+A     LT      AF Q+LLLFH HS DHMG EGQYH++LQ+++ VS  TT++GI L
Sbjct: 121 PRAAASEGLTMLAAAAAFSQQLLLFHFHSTDHMGVEGQYHVILQVVIFVSLLTTIMGIFL 180

Query: 209 PTSFLVAFVRSLSILFQGLWLMLMGFALWTPSFISKG 244
           P SFLV+ VRS SI FQG+WL+++G  L+TPS I KG
Sbjct: 181 PKSFLVSLVRSSSIAFQGVWLIVIGCMLYTPSLIPKG 211

BLAST of Lsi10G009800 vs. TAIR 10
Match: AT5G19870.1 (Family of unknown function (DUF716) )

HSP 1 Score: 248.8 bits (634), Expect = 4.7e-66
Identity = 121/215 (56.28%), Postives = 157/215 (73.02%), Query Frame = 0

Query: 29  MGTLVGHVAPGFGFFIIGLWHLYNNIRIEACKARNDDSSNTAIFLSWFPTSRFKYLEPLL 88
           MGTLVGH+ PGF FF +GLWHL+N+I+I +   +      + I   WFP S+ +YLEPL+
Sbjct: 1   MGTLVGHILPGFFFFALGLWHLFNHIKIFSLHPK------SYIAPLWFPLSKPRYLEPLV 60

Query: 89  IMVGASASIAMELFIGPQRHQPFD-TDGTIPSYHLHNFEHSNISMSFFVFGAFCVILDRL 148
           I++ +S SI++ELF+G + HQPFD  DGTIPS HLHNFEHS IS +F  + AF ++ D+ 
Sbjct: 61  IIIASSLSISVELFLGQKNHQPFDPNDGTIPSNHLHNFEHSCISFTFITYAAFAIVFDKT 120

Query: 149 RPKAYFELTQFVGGVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVLVSFATTLLGIALP 208
           RP A+  L   +  +AF Q+L LFH HS+DH G EG YHLLLQL+V VS  TTLLGIALP
Sbjct: 121 RPIAHRSLINLIAALAFAQQLFLFHFHSSDHTGVEGHYHLLLQLVVFVSLVTTLLGIALP 180

Query: 209 TSFLVAFVRSLSILFQGLWLMLMGFALWTPSFISK 243
           +SF+++FVRSLS+ FQG+WLM M   LWTPS + K
Sbjct: 181 SSFILSFVRSLSVSFQGIWLMSMACMLWTPSLVPK 209

BLAST of Lsi10G009800 vs. TAIR 10
Match: AT1G49470.1 (Family of unknown function (DUF716) )

HSP 1 Score: 135.2 bits (339), Expect = 7.5e-32
Identity = 76/216 (35.19%), Postives = 108/216 (50.00%), Query Frame = 0

Query: 29  MGTLVGHVAPGFGFFIIGLWHLYNNIRIEACKARNDDSSNTAIFLSWFPTSRFKYLEPLL 88
           MGT +GH  PG    ++GLWHL+N IR    K     S+       WFP  + K+LE +L
Sbjct: 1   MGTFLGHFVPGLSLALLGLWHLFNTIRSYCLKGPEAFSAK-----FWFPFPKLKHLELIL 60

Query: 89  IMVGASASIAMELFIGPQRHQPFDTDGTIPSYHLHNFEHSNISMSFFVFGAFCVILD-RL 148
           I+  +  SI +     P     F+     P     N EH+++ +   +F  F +  +  L
Sbjct: 61  ILFFSFLSITLLTLDFPN----FNFSSLKPD----NLEHASMFLHLIIFACFALFCELTL 120

Query: 149 RPKAYFELTQFVGGVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVLVSFATTLLGIALP 208
               +  L   +    F QEL L H HS DH G EG YH LLQL+  VSF++ L   + P
Sbjct: 121 CSDLFSGLIGVLSASVFAQELFLLHFHSTDHSGLEGHYHFLLQLIAFVSFSSALASASFP 180

Query: 209 TSFLVAFVRSLSILFQGLWLMLMGFALWTPSFISKG 244
            SF  A    +S++FQG W + MGF LW P ++ +G
Sbjct: 181 KSFSAALFLPISVMFQGCWFLNMGFMLWIPEYVPRG 203

BLAST of Lsi10G009800 vs. TAIR 10
Match: AT1G32120.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: membrane; EXPRESSED IN: 14 plant structures; EXPRESSED DURING: 4 anthesis, C globular stage, F mature embryo stage, petal differentiation and expansion stage, E expanded cotyledon stage; CONTAINS InterPro DOMAIN/s: Aminotransferase-like, plant mobile domain (InterPro:IPR019557), Protein of unknown function DUF716 (InterPro:IPR006904); BEST Arabidopsis thaliana protein match is: Aminotransferase-like, plant mobile domain family protein (TAIR:AT1G51538.1); Has 16736 Blast hits to 9656 proteins in 576 species: Archae - 4; Bacteria - 1182; Metazoa - 7098; Fungi - 2631; Plants - 1178; Viruses - 174; Other Eukaryotes - 4469 (source: NCBI BLink). )

HSP 1 Score: 109.8 bits (273), Expect = 3.4e-24
Identity = 66/215 (30.70%), Postives = 105/215 (48.84%), Query Frame = 0

Query: 29   MGTLVGHVAPGFGFFIIGLWHLYNNIRIEACKARNDDSSNTAIFLSWFP----TSRFKYL 88
            MG+  GH  PG  F ++G+WH+++++       R   + ++     W P      R KYL
Sbjct: 943  MGSFKGHALPGTLFLVVGVWHIWSSV------VRYISNPSSFRVRVWHPVPGFNDRIKYL 1002

Query: 89   EPLLIMVGASASIAMELFIGPQRHQPFDTDGTIPSYHLHNFEHSNISMSFFVFGAFCVIL 148
            E  ++ +G+   + +E       H  F  +G +   H+++FEHS + + FF+ G   ++ 
Sbjct: 1003 ELYVVTIGSFIDLCIEFLY--STHLKFFVNGVLNPSHMNDFEHSGMLLMFFILGFIALLS 1062

Query: 149  D--RLRPKAYFELTQFVGGVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVLVSFATTLL 208
            +  RL P    E    +   AF  E LLF  HS  H G EG YHLLL  L+ +   +++ 
Sbjct: 1063 EKTRLLPLPQ-EALCLIAATAFTAECLLFFFHSTSHKGLEGYYHLLLVFLIGLCVISSIA 1122

Query: 209  GIALPTSFLVAFVRSLSILFQGLWLMLMGFALWTP 238
            G   PTSF V     +++  QGLW     F L+ P
Sbjct: 1123 GAICPTSFPVDLCNGIAMTLQGLWFYQTAFTLYGP 1148

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9NWC52.4e-0627.70Transmembrane protein 45A OS=Homo sapiens OX=9606 GN=TMEM45A PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3BMV57.7e-10991.16transmembrane protein 45A OS=Cucumis melo OX=3656 GN=LOC103491576 PE=3 SV=1[more]
A0A0A0KX381.8e-10589.30Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G280600 PE=3 SV=1[more]
A0A6J1FXU71.2e-9884.65transmembrane protein 45A-like OS=Cucurbita moschata OX=3662 GN=LOC111448740 PE=... [more]
A0A6J1JGR53.6e-9883.72transmembrane protein 45A-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1JEQ63.6e-9883.72transmembrane protein 45A-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
XP_008449792.11.6e-10891.16PREDICTED: transmembrane protein 45A [Cucumis melo][more]
XP_038901896.14.3e-10690.70transmembrane protein 45B-like [Benincasa hispida][more]
XP_004142158.13.7e-10589.30transmembrane protein 45A [Cucumis sativus] >KGN54083.1 hypothetical protein Csa... [more]
KAG7010234.11.5e-9884.65hypothetical protein SDJN02_27026, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_022944228.12.6e-9884.65transmembrane protein 45A-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT1G55230.13.3e-7262.21Family of unknown function (DUF716) [more]
AT1G55240.19.4e-6757.60Family of unknown function (DUF716) [more]
AT5G19870.14.7e-6656.28Family of unknown function (DUF716) [more]
AT1G49470.17.5e-3235.19Family of unknown function (DUF716) [more]
AT1G32120.13.4e-2430.70FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow... [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006904Protein of unknown function DUF716PFAMPF04819DUF716coord: 155..244
e-value: 2.3E-31
score: 108.3
NoneNo IPR availablePANTHERPTHR46285PROTEINASE INHIBITOR I4, SERPIN (DUF716)-RELATEDcoord: 29..244
NoneNo IPR availablePANTHERPTHR46285:SF3PROTEINASE INHIBITOR I4, SERPIN (DUF716)coord: 29..244

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi10G009800.1Lsi10G009800.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane