Lsi10G009040 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi10G009040
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionL-ascorbate oxidase homolog
Locationchr10: 13501012 .. 13503256 (+)
RNA-Seq ExpressionLsi10G009040
SyntenyLsi10G009040
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAATAATCCATCTTATGTTTTTGAGATACATAGTCTATTCGAGAACAAAAGTGGTGATTTGACAAAAGAAAAAAAAAAACAAAAAAAATATATCGAAAAGAGAGAAAAAGGAAAGATAAAGATGGGTACGATGATGTTCATTGTGTTGCTTTGGTTTTCGGTTGCAACAATAATGTCGACGGTTCGAGGTGAAGATCCTTATTTCTTCTTCACATGGAACATCACCTATGGCACCATCTCTCCCTTGGGCGTTCCTCAACAAGGCATTCTCATCAATGGTCAATTCCCCGGACCTAATATCAACTCCACATCCAACAATAACATTGTCATTAACGTCTTCAACTACCTCAATGAACCCTTTCTTCTACATTGGTATAGTAATTAATGCTTATATTTACATATATTTAACTCTTTTTATTGGTTATCTCGATCACATGATGCAATAAAAAAAAATGGAATATTGGGACTTGCAGGAGCGGTATTCAACACCGAAAGAACTCATGGCAAGATGGACTACCTGGAACCAGTTGCCCAATCCCACCGGGAGCAAACTTCACTTACCATTTCCAAGTTAAGGACCAAATTGGAAGCTTCTTCTACTACCCATCAACTGCCATGCACAGGGCAGCGGGAGGCTTCGGTGGGCTCCGTGTGAACAGTCGTTTACTCATTCCTGTCCCTTATGCTGACCCTGAAGATGATTACACTGTCCTTATTGGTGATTGGTATACCAAGAGCCACACCACTCTTAAGCAGATCTTAGACAATGGTCATCGCTCCATTGCTCGACCTGAGGGTGTCTTAATCAACGGGAAGATTGCGAAAGGTGATGGAACCGATGAGCCACTCTTCACCATGAAGCCTGGAAAGACTTACAAGTATCGAGTTTGCAATGTGGGACTGAAGACATCTCTCAATTTTAGATTCCAAGGACACACAATGAAGTTGGTAGAGATGGAGGGGTCTCATACCGTGCAAAATGATTATCAATCACTTGACGTCCATGTGGGACAATGCTTTTCAGTGCTAGTCACAGCCGACAAGGAGCCCAGAGATTATTACATGGTTGCATCAACTAGGTTCATTAAGAACGTTGTTATTGGTAAAGGCATTGTGAGATACACCAATGGCAAAGGTCCTGCCTCTCCTGAAATCCCTAAGGCACCCGTGGGTTGGGCTTGGTCCCTCAATCAATTCCGTACCTTCCGTTGGAACCTTACTGCTAGTGCTGCAAGGCCTAATCCTCAAGGCTCTTACCATTATGGTTCCATCAACATTACTCGTACCATCAGGCTATTCAATTCAGCTACTAAGGTGGATGGTAAATTGCGATATGCCATCAATGGTGTCTCCCATGTTGATCCTGAAACTCCCTTGAAGCTTGCAGAATACTTTGGAATTGCTGACAAAGTCTTCAAGTACGATACCATTTCTGATGAGGGGCCAGCAGAAGGTGCAACCACTATGACTATTGCTCCTAATGTTGTTAATGCAACCTTCCGCAACTTCGTAGAGATTATTTTTGAGAATCACGAGAAGAGCCTCCAGTCATGGCATTTAGATGGCTACTCTTTCTTCGCTGTTGCGTAAGTATCATCAGCTTGAAAAAAAATTAGTGTGTATATAAGTATAAATTGTGTATTCCTAAATTTAATGATAGATACTGAGAATTCTTGTTTAATGTTTCACAGCATTGAGCCTGGGAGATGGTCTCCAGAGAAACGATCGAACTATAATCTTCTAGATGCCGTGAGCAGGCATACAATCCAAGTCTTCCCTAATTCATGGGCAGCCATACTTCTTACATTTGACAATGCTGGAATGTGGAACTTGAGGTCTGAGTTGATAGAAAATCGTTACTTGGGACAACAACTTTACATTAGTGTTCTTTCACCAGCTCGCTCCCTTAGAGATGAGTACAACATCCCAGATCACACTTTGCTCTGTGGTTTGGTGAAGGGCATGCCATTGCCCAAGCCATATACCATTTGAAATTGTTCATCTACCCTTCCTCAACTCCAATTCTGAAAATATGATCGATACACATCAAGCATTTTGGAGAGGTTAAATTTTCATGCATGAATGTCGCATGTAATTAAAAGTGGAAGATTTTCTTCACTGAGCCTTCTTGGTTTTTTTTTTTTTTTTTGTCTCTTTTTTCTTTTTATACGATGAATTTTGAAATAAAACACAGATTCATTTGCATCAATTAAGCTTTCCTTTGTTGTTATCATCGTACGTGCAC

mRNA sequence

CAATAATCCATCTTATGTTTTTGAGATACATAGTCTATTCGAGAACAAAAGTGGTGATTTGACAAAAGAAAAAAAAAAACAAAAAAAATATATCGAAAAGAGAGAAAAAGGAAAGATAAAGATGGGTACGATGATGTTCATTGTGTTGCTTTGGTTTTCGGTTGCAACAATAATGTCGACGGTTCGAGGTGAAGATCCTTATTTCTTCTTCACATGGAACATCACCTATGGCACCATCTCTCCCTTGGGCGTTCCTCAACAAGGCATTCTCATCAATGGTCAATTCCCCGGACCTAATATCAACTCCACATCCAACAATAACATTGTCATTAACGTCTTCAACTACCTCAATGAACCCTTTCTTCTACATTGGAGCGGTATTCAACACCGAAAGAACTCATGGCAAGATGGACTACCTGGAACCAGTTGCCCAATCCCACCGGGAGCAAACTTCACTTACCATTTCCAAGTTAAGGACCAAATTGGAAGCTTCTTCTACTACCCATCAACTGCCATGCACAGGGCAGCGGGAGGCTTCGGTGGGCTCCGTGTGAACAGTCGTTTACTCATTCCTGTCCCTTATGCTGACCCTGAAGATGATTACACTGTCCTTATTGGTGATTGGTATACCAAGAGCCACACCACTCTTAAGCAGATCTTAGACAATGGTCATCGCTCCATTGCTCGACCTGAGGGTGTCTTAATCAACGGGAAGATTGCGAAAGGTGATGGAACCGATGAGCCACTCTTCACCATGAAGCCTGGAAAGACTTACAAGTATCGAGTTTGCAATGTGGGACTGAAGACATCTCTCAATTTTAGATTCCAAGGACACACAATGAAGTTGGTAGAGATGGAGGGGTCTCATACCGTGCAAAATGATTATCAATCACTTGACGTCCATGTGGGACAATGCTTTTCAGTGCTAGTCACAGCCGACAAGGAGCCCAGAGATTATTACATGGTTGCATCAACTAGGTTCATTAAGAACGTTGTTATTGGTAAAGGCATTGTGAGATACACCAATGGCAAAGGTCCTGCCTCTCCTGAAATCCCTAAGGCACCCGTGGGTTGGGCTTGGTCCCTCAATCAATTCCGTACCTTCCGTTGGAACCTTACTGCTAGTGCTGCAAGGCCTAATCCTCAAGGCTCTTACCATTATGGTTCCATCAACATTACTCGTACCATCAGGCTATTCAATTCAGCTACTAAGGTGGATGGTAAATTGCGATATGCCATCAATGGTGTCTCCCATGTTGATCCTGAAACTCCCTTGAAGCTTGCAGAATACTTTGGAATTGCTGACAAAGTCTTCAAGTACGATACCATTTCTGATGAGGGGCCAGCAGAAGGTGCAACCACTATGACTATTGCTCCTAATGTTGTTAATGCAACCTTCCGCAACTTCGTAGAGATTATTTTTGAGAATCACGAGAAGAGCCTCCAGTCATGGCATTTAGATGGCTACTCTTTCTTCGCTGTTGCCATTGAGCCTGGGAGATGGTCTCCAGAGAAACGATCGAACTATAATCTTCTAGATGCCGTGAGCAGGCATACAATCCAAGTCTTCCCTAATTCATGGGCAGCCATACTTCTTACATTTGACAATGCTGGAATGTGGAACTTGAGGTCTGAGTTGATAGAAAATCGTTACTTGGGACAACAACTTTACATTAGTGTTCTTTCACCAGCTCGCTCCCTTAGAGATGAGTACAACATCCCAGATCACACTTTGCTCTGTGGTTTGGTGAAGGGCATGCCATTGCCCAAGCCATATACCATTTGAAATTGTTCATCTACCCTTCCTCAACTCCAATTCTGAAAATATGATCGATACACATCAAGCATTTTGGAGAGGTTAAATTTTCATGCATGAATGTCGCATGTAATTAAAAGTGGAAGATTTTCTTCACTGAGCCTTCTTGGTTTTTTTTTTTTTTTTTGTCTCTTTTTTCTTTTTATACGATGAATTTTGAAATAAAACACAGATTCATTTGCATCAATTAAGCTTTCCTTTGTTGTTATCATCGTACGTGCAC

Coding sequence (CDS)

ATGGGTACGATGATGTTCATTGTGTTGCTTTGGTTTTCGGTTGCAACAATAATGTCGACGGTTCGAGGTGAAGATCCTTATTTCTTCTTCACATGGAACATCACCTATGGCACCATCTCTCCCTTGGGCGTTCCTCAACAAGGCATTCTCATCAATGGTCAATTCCCCGGACCTAATATCAACTCCACATCCAACAATAACATTGTCATTAACGTCTTCAACTACCTCAATGAACCCTTTCTTCTACATTGGAGCGGTATTCAACACCGAAAGAACTCATGGCAAGATGGACTACCTGGAACCAGTTGCCCAATCCCACCGGGAGCAAACTTCACTTACCATTTCCAAGTTAAGGACCAAATTGGAAGCTTCTTCTACTACCCATCAACTGCCATGCACAGGGCAGCGGGAGGCTTCGGTGGGCTCCGTGTGAACAGTCGTTTACTCATTCCTGTCCCTTATGCTGACCCTGAAGATGATTACACTGTCCTTATTGGTGATTGGTATACCAAGAGCCACACCACTCTTAAGCAGATCTTAGACAATGGTCATCGCTCCATTGCTCGACCTGAGGGTGTCTTAATCAACGGGAAGATTGCGAAAGGTGATGGAACCGATGAGCCACTCTTCACCATGAAGCCTGGAAAGACTTACAAGTATCGAGTTTGCAATGTGGGACTGAAGACATCTCTCAATTTTAGATTCCAAGGACACACAATGAAGTTGGTAGAGATGGAGGGGTCTCATACCGTGCAAAATGATTATCAATCACTTGACGTCCATGTGGGACAATGCTTTTCAGTGCTAGTCACAGCCGACAAGGAGCCCAGAGATTATTACATGGTTGCATCAACTAGGTTCATTAAGAACGTTGTTATTGGTAAAGGCATTGTGAGATACACCAATGGCAAAGGTCCTGCCTCTCCTGAAATCCCTAAGGCACCCGTGGGTTGGGCTTGGTCCCTCAATCAATTCCGTACCTTCCGTTGGAACCTTACTGCTAGTGCTGCAAGGCCTAATCCTCAAGGCTCTTACCATTATGGTTCCATCAACATTACTCGTACCATCAGGCTATTCAATTCAGCTACTAAGGTGGATGGTAAATTGCGATATGCCATCAATGGTGTCTCCCATGTTGATCCTGAAACTCCCTTGAAGCTTGCAGAATACTTTGGAATTGCTGACAAAGTCTTCAAGTACGATACCATTTCTGATGAGGGGCCAGCAGAAGGTGCAACCACTATGACTATTGCTCCTAATGTTGTTAATGCAACCTTCCGCAACTTCGTAGAGATTATTTTTGAGAATCACGAGAAGAGCCTCCAGTCATGGCATTTAGATGGCTACTCTTTCTTCGCTGTTGCCATTGAGCCTGGGAGATGGTCTCCAGAGAAACGATCGAACTATAATCTTCTAGATGCCGTGAGCAGGCATACAATCCAAGTCTTCCCTAATTCATGGGCAGCCATACTTCTTACATTTGACAATGCTGGAATGTGGAACTTGAGGTCTGAGTTGATAGAAAATCGTTACTTGGGACAACAACTTTACATTAGTGTTCTTTCACCAGCTCGCTCCCTTAGAGATGAGTACAACATCCCAGATCACACTTTGCTCTGTGGTTTGGTGAAGGGCATGCCATTGCCCAAGCCATATACCATTTGA

Protein sequence

MGTMMFIVLLWFSVATIMSTVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNINSTSNNNIVINVFNYLNEPFLLHWSGIQHRKNSWQDGLPGTSCPIPPGANFTYHFQVKDQIGSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQILDNGHRSIARPEGVLINGKIAKGDGTDEPLFTMKPGKTYKYRVCNVGLKTSLNFRFQGHTMKLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTADKEPRDYYMVASTRFIKNVVIGKGIVRYTNGKGPASPEIPKAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIRLFNSATKVDGKLRYAINGVSHVDPETPLKLAEYFGIADKVFKYDTISDEGPAEGATTMTIAPNVVNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRSNYNLLDAVSRHTIQVFPNSWAAILLTFDNAGMWNLRSELIENRYLGQQLYISVLSPARSLRDEYNIPDHTLLCGLVKGMPLPKPYTI
Homology
BLAST of Lsi10G009040 vs. ExPASy Swiss-Prot
Match: Q00624 (L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1)

HSP 1 Score: 887.9 bits (2293), Expect = 6.0e-257
Identity = 405/552 (73.37%), Postives = 465/552 (84.24%), Query Frame = 0

Query: 2   GTMMFIVLLWFSVATIMSTVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNIN 61
           G  +    L+ + A  +  V  EDPYF   WN+TYGT SPLGVPQQ ILINGQFPGPNIN
Sbjct: 3   GVKLLAACLYLAAAATV-VVHAEDPYFHHVWNVTYGTASPLGVPQQVILINGQFPGPNIN 62

Query: 62  STSNNNIVINVFNYLNEPFLLHWSGIQHRKNSWQDGLPGTSCPIPPGANFTYHFQVKDQI 121
           STSNNN++INVFN L+EPFLL W+GIQHRKN WQDG PGT CPI PG N+TYHFQ KDQI
Sbjct: 63  STSNNNVIINVFNNLDEPFLLTWNGIQHRKNCWQDGTPGTMCPIMPGTNYTYHFQPKDQI 122

Query: 122 GSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQILD 181
           GS+FYYP+T MHRAAGG+GGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHT LK+ LD
Sbjct: 123 GSYFYYPTTGMHRAAGGYGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTQLKKFLD 182

Query: 182 NGHRSIARPEGVLINGKIAKGDGTDEPLFTMKPGKTYKYRVCNVGLKTSLNFRFQGHTMK 241
            G R+I RP+G++INGK  KGDG+D PLFT+KPGKTY+ R+CNVG+KTS+NFR Q H MK
Sbjct: 183 GG-RTIGRPDGIVINGKSGKGDGSDAPLFTLKPGKTYRVRICNVGVKTSINFRIQNHKMK 242

Query: 242 LVEMEGSHTVQNDYQSLDVHVGQCFSVLVTADKEPRDYYMVASTRFIKNVVIGKGIVRYT 301
           LVEMEGSH +QNDY SLDVHVGQCF  +VTA++EP+DYYMVAS+RF+K V+   G++RY 
Sbjct: 243 LVEMEGSHVLQNDYDSLDVHVGQCFGTIVTANQEPKDYYMVASSRFLKTVITTTGLLRYE 302

Query: 302 NGKGPASPEIPKAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIRLFNS 361
            GKGPAS ++P  PVGWAWSLNQFR+FRWNLTASAARPNPQGSYHYG INITRTI+L N+
Sbjct: 303 GGKGPASSQLPAGPVGWAWSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVNT 362

Query: 362 ATKVDGKLRYAINGVSHVDPETPLKLAEYFGIADKVFKYDTISDEGPAEGATTMTIAPNV 421
             KVDGKLR+A+NGVSH +PETPLKLAEYFGI+DKVFKYDTI+D+   E    + I PNV
Sbjct: 363 QGKVDGKLRFALNGVSHTEPETPLKLAEYFGISDKVFKYDTITDDPTPEQIKNIKIEPNV 422

Query: 422 VNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRSNYNLLDAVSRHTIQ 481
           +N T R FVE++FENHEKS+QSWHLDGYSFF+VA+EPG W+PEKR NYNLLDAVSRHT+Q
Sbjct: 423 LNITHRTFVEVVFENHEKSVQSWHLDGYSFFSVAVEPGTWTPEKRKNYNLLDAVSRHTVQ 482

Query: 482 VFPNSWAAILLTFDNAGMWNLRSELIENRYLGQQLYISVLSPARSLRDEYNIPDHTLLCG 541
           V+P  WAAILLTFDN GMWN+RSE  E RYLGQQLY SVLSP +SLRDEYN+P+ +L CG
Sbjct: 483 VYPKCWAAILLTFDNCGMWNVRSENTERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCG 542

Query: 542 LVKGMPLP-KPY 553
           LVK  P P  PY
Sbjct: 543 LVKNTPKPVNPY 552

BLAST of Lsi10G009040 vs. ExPASy Swiss-Prot
Match: P29162 (L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 834.7 bits (2155), Expect = 6.0e-241
Identity = 384/556 (69.06%), Postives = 460/556 (82.73%), Query Frame = 0

Query: 2   GTMMFI-VLLWFSVATIMSTVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNI 61
           G + F+ +LL  SV  I      EDPY +F WN+TYGTI+PLGVPQQGILINGQFPGP I
Sbjct: 4   GKVTFVALLLCLSVGVI-----AEDPYLYFNWNVTYGTIAPLGVPQQGILINGQFPGPRI 63

Query: 62  NSTSNNNIVINVFNYLNEPFLLHWSGIQHRKNSWQDGLPGTSCPIPPGANFTYHFQVKDQ 121
           N TSNNNIV+NVFN L+EPFL  W+G+QHRKNSWQDG PGT CPI PG NFTY FQVKDQ
Sbjct: 64  NCTSNNNIVVNVFNNLDEPFLFTWNGVQHRKNSWQDGTPGTMCPIMPGQNFTYRFQVKDQ 123

Query: 122 IGSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQIL 181
           IGS+ Y+P+TA+HRAAGG+G L V+SR LIPVP+ +P D+Y V +GDWY K H TLK+IL
Sbjct: 124 IGSYSYFPTTALHRAAGGYGALNVHSRALIPVPFDNPADEYNVFVGDWYNKGHKTLKKIL 183

Query: 182 DNGHRSIARPEGVLINGKIAKGDGTDEPLFTMKPGKTYKYRVCNVGLKTSLNFRFQGHTM 241
           D G R+I RP+G++INGK AK     EPLFTM+ GKTY+YR CN+G+++S+N RFQGH M
Sbjct: 184 DGG-RTIGRPDGIIINGKSAKVGEAKEPLFTMEAGKTYRYRFCNLGMRSSVNIRFQGHPM 243

Query: 242 KLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTADKEPRDYYMVASTRFIKNVVIGKGIVRY 301
           KLVE+EGSHTVQN Y SLD+HVGQC SVLVTAD+EP+DYY+V S+RF+K  +    I+RY
Sbjct: 244 KLVELEGSHTVQNIYDSLDLHVGQCLSVLVTADQEPKDYYLVVSSRFLKQALSSVAIIRY 303

Query: 302 TNGKGPASPEIPKAP----VGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTI 361
            NGKGPASPE+P  P     G AWS+NQFR+FRWNLTASAARPNPQGSYHYG INITRTI
Sbjct: 304 ANGKGPASPELPTPPPENTEGIAWSMNQFRSFRWNLTASAARPNPQGSYHYGQINITRTI 363

Query: 362 RLFNSATKVDGKLRYAINGVSHVDPETPLKLAEYFGIADKVFKYDTISDEGPAEGATTMT 421
           ++FNS ++V GKLRY +NG+SH + ETPLKL EYFG  +K FKYD ++DE PA+  + +T
Sbjct: 364 KIFNSMSQVGGKLRYGLNGISHTNGETPLKLVEYFGATNKAFKYDLMADEAPAD-PSKLT 423

Query: 422 IAPNVVNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRSNYNLLDAVS 481
           IA NV NAT+RNFVEIIFENHEK+++++HLDGYSFFAVA+EPGRWSPEKR NYNL+D +S
Sbjct: 424 IATNVKNATYRNFVEIIFENHEKTIRTYHLDGYSFFAVAVEPGRWSPEKRKNYNLVDGLS 483

Query: 482 RHTIQVFPNSWAAILLTFDNAGMWNLRSELIENRYLGQQLYISVLSPARSLRDEYNIPDH 541
           R+ IQV+PNSWAAI+LTFDNAGMWNLRSE+ E  YLG+QLY SVLSP+RSLRDEYNIPD+
Sbjct: 484 RNNIQVYPNSWAAIMLTFDNAGMWNLRSEMWEKTYLGEQLYFSVLSPSRSLRDEYNIPDN 543

Query: 542 TLLCGLVKGMPLPKPY 553
             LCG+VKG+ +P PY
Sbjct: 544 HPLCGIVKGLSMPAPY 552

BLAST of Lsi10G009040 vs. ExPASy Swiss-Prot
Match: Q9SU40 (Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=1 SV=1)

HSP 1 Score: 552.4 bits (1422), Expect = 6.0e-156
Identity = 271/562 (48.22%), Postives = 373/562 (66.37%), Query Frame = 0

Query: 7   IVLLWFSVATIMSTVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNINSTSNN 66
           I+LL F V   +S     DPY F+ + ++Y T SPLGVPQQ I ING+FPGP IN T+N 
Sbjct: 6   ILLLVFFVN--ISFCFAADPYSFYNFEVSYITASPLGVPQQVIAINGKFPGPTINVTTNE 65

Query: 67  NIVINVFNYLNEPFLLHWSGIQHRKNSWQDGLPGTSCPIPPGANFTYHFQVKDQIGSFFY 126
           N+V+NV N L+E  LLHW+GIQ R+ SWQDG+ GT+CPIPP  N+TY FQVKDQIGSFFY
Sbjct: 66  NLVVNVRNKLDEGLLLHWNGIQQRRVSWQDGVLGTNCPIPPKWNWTYEFQVKDQIGSFFY 125

Query: 127 YPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQILDNGHRS 186
           +PS    RA+GGFG   VN R +IPVP++ P+ D TV IGDWY ++HT L++ LD+G + 
Sbjct: 126 FPSLHFQRASGGFGSFVVNPRAIIPVPFSTPDGDITVTIGDWYIRNHTALRKALDDG-KD 185

Query: 187 IARPEGVLINGK-------IAKGDGTDEPLFTMKPGKTYKYRVCNVGLKTSLNFRFQGHT 246
           +  P+GVLINGK           DG D    T+ PGKTY+ RV NVG+ TSLNFR QGH 
Sbjct: 186 LGMPDGVLINGKGPYRYNDTLVADGIDFETITVHPGKTYRLRVSNVGISTSLNFRIQGHN 245

Query: 247 MKLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTADKE-PRDYYMVASTRFIKNV----VIG 306
           + L E EGS+TVQ +Y SLD+HVGQ +S LVT D+    DYY+VAS R +       V G
Sbjct: 246 LVLAESEGSYTVQQNYTSLDIHVGQSYSFLVTMDQNASSDYYIVASARVVNETIWRRVTG 305

Query: 307 KGIVRYTNGKGPASPEIPKAP---VGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSIN 366
            GI++YTN KG A  ++P  P       +S+NQ R+ RWN++AS ARPNPQGS+ YGSIN
Sbjct: 306 VGILKYTNSKGKAKGQLPPGPQDEFDKTFSMNQARSIRWNVSASGARPNPQGSFKYGSIN 365

Query: 367 ITRTIRLFN-SATKVDGKLRYAINGVSHVDPETPLKLAEYFGIADKVFKYDTISD--EGP 426
           +T    L N     + GK R  +NG+S  +P TP++LA+   + D V+K D       GP
Sbjct: 366 VTDVYVLRNMPPVTISGKRRTTLNGISFKNPSTPIRLADKLKVKD-VYKLDFPKRPLTGP 425

Query: 427 AEGATTMTIAPNVVNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRSN 486
           A+      +A +++N T+R F+E++ +N++  +QS+H+ GY+FF V ++ G W+   R  
Sbjct: 426 AK------VATSIINGTYRGFMEVVLQNNDTKMQSYHMSGYAFFVVGMDYGEWTENSRGT 485

Query: 487 YNLLDAVSRHTIQVFPNSWAAILLTFDNAGMWNLRSELIENRYLGQQLYISVLSPARSLR 546
           YN  D ++R TIQV+P +W+AIL++ DN G WNLR+E +++ YLGQ+ Y+ V++P  + +
Sbjct: 486 YNKWDGIARSTIQVYPGAWSAILISLDNPGAWNLRTENLDSWYLGQETYVRVVNPDENNK 545

Query: 547 DEYNIPDHTLLCGLVKGMPLPK 551
            E+  PD+ L CG +  +  P+
Sbjct: 546 TEFGHPDNVLYCGALSKLQKPQ 557

BLAST of Lsi10G009040 vs. ExPASy Swiss-Prot
Match: Q9FHN6 (Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=2 SV=1)

HSP 1 Score: 536.2 bits (1380), Expect = 4.4e-151
Identity = 260/563 (46.18%), Postives = 376/563 (66.79%), Query Frame = 0

Query: 1   MGTMMFIVLLWFSVATIMSTVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNI 60
           M    F     FS A I       DPY  + + ++Y T SPLGVPQQ I +NG+FPGP I
Sbjct: 1   MAATDFFFAFVFSFALIFGFSFAGDPYVSYDFTLSYITASPLGVPQQVIAVNGKFPGPVI 60

Query: 61  NSTSNNNIVINVFNYLNEPFLLHWSGIQHRKNSWQDGLPGTSCPIPPGANFTYHFQVKDQ 120
           N+T+N N+ +NV N+L+EP LL W G+Q R+NSWQDG+ GT+CPIPP  NFTY FQ+KDQ
Sbjct: 61  NATTNYNVHVNVLNHLDEPLLLTWPGVQMRRNSWQDGVLGTNCPIPPNWNFTYDFQLKDQ 120

Query: 121 IGSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQIL 180
           IGS+FY PS    RA+GGFG L +N+R L+P+P+ +P+ +   +IGDWYT++HT L++IL
Sbjct: 121 IGSYFYSPSLNFQRASGGFGALIINNRDLVPIPFTEPDGEIIFIIGDWYTQNHTALRRIL 180

Query: 181 DNGHRSIARPEGVLINGK------IAKGDGTDEPLFTMKPGKTYKYRVCNVGLKTSLNFR 240
           D+G + +  P+GVLINGK       +  DG +     + PGKTY+ RV NVG+ TSLNFR
Sbjct: 181 DSG-KELGMPDGVLINGKGPFKYNSSVPDGIEHETVNVDPGKTYRIRVHNVGISTSLNFR 240

Query: 241 FQGHTMKLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTADKE-PRDYYMVASTRFIKNV-- 300
            Q H + L+E EG +T Q ++   DVHVGQ +S LVT D+    DYY+VAS RF+     
Sbjct: 241 IQNHKLLLIETEGRYTSQMNFTDFDVHVGQSYSFLVTMDQNATSDYYIVASARFVNETVW 300

Query: 301 --VIGKGIVRYTNGKGPASPEIP--KAPVGWAWS-LNQFRTFRWNLTASAARPNPQGSYH 360
             V G GI+ Y+N KGPAS  +P     V   WS +NQ R  + N +AS ARPNPQGS+H
Sbjct: 301 QRVTGVGILHYSNSKGPASGPLPVSATDVNHPWSAMNQPRAIKQNTSASGARPNPQGSFH 360

Query: 361 YGSINITRTIRLFN-SATKVDGKLRYAINGVSHVDPETPLKLAEYFGIADKVFKYDTISD 420
           YG INITRT  L +   TK++GKLR  +NG+S V+P TP++LA+     D   K D + D
Sbjct: 361 YGQINITRTYILRSLPPTKINGKLRATLNGISFVNPSTPMRLAD-----DHKVKGDYMLD 420

Query: 421 --EGPAEGATTMTIAPNVVNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSP 480
             + P +      ++ +++NAT++ F+++IF+N++  +QS+H+DGY+F+ VA++ G WS 
Sbjct: 421 FPDRPLD-EKLPRLSSSIINATYKGFIQVIFQNNDTKIQSFHIDGYAFYVVAMDFGIWSE 480

Query: 481 EKRSNYNLLDAVSRHTIQVFPNSWAAILLTFDNAGMWNLRSELIENRYLGQQLYISVLSP 540
           ++ S+YN  DAV+R T++V+P +W A+L++ DN G+WN+R E ++  YLGQ+ Y+ +++P
Sbjct: 481 DRNSSYNNWDAVARSTVEVYPGAWTAVLISLDNVGVWNIRVENLDRWYLGQETYMRIINP 540

Query: 541 ARSLRDEYNIPDHTLLCGLVKGM 547
             +   E + P++ + CG ++ M
Sbjct: 541 EENGSTEMDPPENVMYCGALQAM 556

BLAST of Lsi10G009040 vs. ExPASy Swiss-Prot
Match: Q8VXX5 (Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=2 SV=1)

HSP 1 Score: 528.1 bits (1359), Expect = 1.2e-148
Identity = 258/553 (46.65%), Postives = 372/553 (67.27%), Query Frame = 0

Query: 9   LLWFSVATIMSTVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNINSTSNNNI 68
           LL F++ + +S     DP+  + + ++Y T SPLGVPQQ I +NGQFPGP +N+T+N N+
Sbjct: 12  LLCFALLSAVSF--AADPFVSYDFRVSYLTASPLGVPQQVIAVNGQFPGPLLNATTNYNV 71

Query: 69  VINVFNYLNEPFLLHWSGIQHRKNSWQDGLPGTSCPIPPGANFTYHFQVKDQIGSFFYYP 128
           V+NVFN+L+EP LL W GIQ R+NSWQDG+ GT+CPIPP  NFTY FQVKDQIGSFFY P
Sbjct: 72  VVNVFNHLDEPLLLTWPGIQMRRNSWQDGVLGTNCPIPPRWNFTYQFQVKDQIGSFFYSP 131

Query: 129 STAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQILDNGHRSIA 188
           S    RA+GGFG + +N+R +IP+P+  P+ +   +IGDWYT+ H  L++ LD+G + + 
Sbjct: 132 SLNFQRASGGFGPIVINNRDIIPIPFPQPDGELIFIIGDWYTQDHKALRRALDSG-KELG 191

Query: 189 RPEGVLINGK------IAKGDGTDEPLFTMKPGKTYKYRVCNVGLKTSLNFRFQGHTMKL 248
            P+GVLINGK       +  DG D   F ++PGKTY+ RV NVG+ TSLNFR Q H++ L
Sbjct: 192 MPDGVLINGKGPYKYNSSVPDGIDYLTFHVEPGKTYRIRVHNVGISTSLNFRIQNHSLLL 251

Query: 249 VEMEGSHTVQNDYQSLDVHVGQCFSVLVTADKE-PRDYYMVASTRFIKNV----VIGKGI 308
           VE EG +T Q ++   DVHVGQ +S LVT D++   DYY+VAS RF+       V G  I
Sbjct: 252 VETEGHYTSQANFTDFDVHVGQSYSFLVTMDQDATSDYYIVASARFVNETVWQRVTGVAI 311

Query: 309 VRYTNGKGPASP--EIPKAPVGWAWS-LNQFRTFRWNLTASAARPNPQGSYHYGSINITR 368
           + Y+N KGP S    +PK  V   WS ++Q +T R N +AS ARPNPQGS+HYG INIT 
Sbjct: 312 LHYSNSKGPVSGPLPVPKTDVSSPWSAMSQPKTIRQNTSASGARPNPQGSFHYGQINITN 371

Query: 369 TIRLFN-SATKVDGKLRYAINGVSHVDPETPLKLAEYFGIADKVFKYDTISDEGPAEGAT 428
           T  L +   T ++G LR  +NG+S V+P TP++LA+   +    +K D    + P     
Sbjct: 372 TYILRSLPPTIINGALRATLNGISFVNPSTPVRLADRNKVKG-AYKLD--FPDRPFN--R 431

Query: 429 TMTIAPNVVNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRSNYNLLD 488
            + +  +++NAT++ F++++F+N++  +QS+H+DGYSFF V ++ G WS +K+ +YN  D
Sbjct: 432 PLRLDRSMINATYKGFIQVVFQNNDTKIQSFHVDGYSFFVVGMDFGIWSEDKKGSYNNWD 491

Query: 489 AVSRHTIQVFPNSWAAILLTFDNAGMWNLRSELIENRYLGQQLYISVLSPARSLRDEYNI 547
           A+SR TI+V+P  W A+L++ DN G+WN+R E ++  YLG++ Y+ + +P    + E + 
Sbjct: 492 AISRSTIEVYPGGWTAVLISLDNVGVWNIRVENLDRWYLGEETYMRITNPEEDGKTEMDP 551

BLAST of Lsi10G009040 vs. ExPASy TrEMBL
Match: A0A5A7TF08 (L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold366G001020 PE=3 SV=1)

HSP 1 Score: 1112.1 bits (2875), Expect = 0.0e+00
Identity = 524/554 (94.58%), Postives = 539/554 (97.29%), Query Frame = 0

Query: 1   MGTMMFIVLLWFSVATIMSTVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNI 60
           M TMMF VLL  S AT MS VRGEDPYFFFTWN+TYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 2   MATMMFTVLLCLSAAT-MSMVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 61

Query: 61  NSTSNNNIVINVFNYLNEPFLLHWSGIQHRKNSWQDGLPGTSCPIPPGANFTYHFQVKDQ 120
           NSTSNNNIV+NVFN LNEPFLLHWSGIQHRKNSWQDGL GT+CPIPPGANFTYHFQVKDQ
Sbjct: 62  NSTSNNNIVVNVFNNLNEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGANFTYHFQVKDQ 121

Query: 121 IGSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQIL 180
           IGSFFYYPS AMHRAAGGFGGLR+NSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQIL
Sbjct: 122 IGSFFYYPSIAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQIL 181

Query: 181 DNGHRSIARPEGVLINGKIAKGDGTDEPLFTMKPGKTYKYRVCNVGLKTSLNFRFQGHTM 240
           DNGHRSIARPEGVLINGK AKGDGTDEPLFTMKPGKTYKYRVCNVGLKTSLNFRFQGHTM
Sbjct: 182 DNGHRSIARPEGVLINGKTAKGDGTDEPLFTMKPGKTYKYRVCNVGLKTSLNFRFQGHTM 241

Query: 241 KLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTADKEPRDYYMVASTRFIKNVVIGKGIVRY 300
           KLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTA++EP+DYYMVASTRFIKNV+IGKGI+RY
Sbjct: 242 KLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTANQEPKDYYMVASTRFIKNVIIGKGIIRY 301

Query: 301 TNGKGPASPEIPKAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIRLFN 360
           TNGKGPASPEIPKAP+GWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTI+LFN
Sbjct: 302 TNGKGPASPEIPKAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLFN 361

Query: 361 SATKVDGKLRYAINGVSHVDPETPLKLAEYFGIADKVFKYDTISDEGPAEGATTMTIAPN 420
           SATKVDGKLRYAINGVSH+DPETPLKLAEYFGIADKVFKYD ISDEGP EGAT +TIAPN
Sbjct: 362 SATKVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDIISDEGPTEGATAVTIAPN 421

Query: 421 VVNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRSNYNLLDAVSRHTI 480
           VVNATFRNF+EIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRSNYNLLDAVSRHTI
Sbjct: 422 VVNATFRNFIEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRSNYNLLDAVSRHTI 481

Query: 481 QVFPNSWAAILLTFDNAGMWNLRSELIENRYLGQQLYISVLSPARSLRDEYNIPDHTLLC 540
           QVFPNSWAAILLTFDNAGMWNLRSEL ENRYLGQQLY+SVLSPARSLRDEYNIPDHTLLC
Sbjct: 482 QVFPNSWAAILLTFDNAGMWNLRSELTENRYLGQQLYMSVLSPARSLRDEYNIPDHTLLC 541

Query: 541 GLVKGMPLPKPYTI 555
           GLVKGMPLPKPYTI
Sbjct: 542 GLVKGMPLPKPYTI 554

BLAST of Lsi10G009040 vs. ExPASy TrEMBL
Match: A0A1S3BNS2 (L-ascorbate oxidase homolog OS=Cucumis melo OX=3656 GN=LOC103491644 PE=3 SV=1)

HSP 1 Score: 1112.1 bits (2875), Expect = 0.0e+00
Identity = 524/554 (94.58%), Postives = 539/554 (97.29%), Query Frame = 0

Query: 1   MGTMMFIVLLWFSVATIMSTVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNI 60
           M TMMF VLL  S AT MS VRGEDPYFFFTWN+TYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 1   MATMMFTVLLCLSAAT-MSMVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTSNNNIVINVFNYLNEPFLLHWSGIQHRKNSWQDGLPGTSCPIPPGANFTYHFQVKDQ 120
           NSTSNNNIV+NVFN LNEPFLLHWSGIQHRKNSWQDGL GT+CPIPPGANFTYHFQVKDQ
Sbjct: 61  NSTSNNNIVVNVFNNLNEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGANFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQIL 180
           IGSFFYYPS AMHRAAGGFGGLR+NSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQIL
Sbjct: 121 IGSFFYYPSIAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQIL 180

Query: 181 DNGHRSIARPEGVLINGKIAKGDGTDEPLFTMKPGKTYKYRVCNVGLKTSLNFRFQGHTM 240
           DNGHRSIARPEGVLINGK AKGDGTDEPLFTMKPGKTYKYRVCNVGLKTSLNFRFQGHTM
Sbjct: 181 DNGHRSIARPEGVLINGKTAKGDGTDEPLFTMKPGKTYKYRVCNVGLKTSLNFRFQGHTM 240

Query: 241 KLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTADKEPRDYYMVASTRFIKNVVIGKGIVRY 300
           KLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTA++EP+DYYMVASTRFIKNV+IGKGI+RY
Sbjct: 241 KLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTANQEPKDYYMVASTRFIKNVIIGKGIIRY 300

Query: 301 TNGKGPASPEIPKAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIRLFN 360
           TNGKGPASPEIPKAP+GWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTI+LFN
Sbjct: 301 TNGKGPASPEIPKAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLFN 360

Query: 361 SATKVDGKLRYAINGVSHVDPETPLKLAEYFGIADKVFKYDTISDEGPAEGATTMTIAPN 420
           SATKVDGKLRYAINGVSH+DPETPLKLAEYFGIADKVFKYD ISDEGP EGAT +TIAPN
Sbjct: 361 SATKVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDIISDEGPTEGATAVTIAPN 420

Query: 421 VVNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRSNYNLLDAVSRHTI 480
           VVNATFRNF+EIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRSNYNLLDAVSRHTI
Sbjct: 421 VVNATFRNFIEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRSNYNLLDAVSRHTI 480

Query: 481 QVFPNSWAAILLTFDNAGMWNLRSELIENRYLGQQLYISVLSPARSLRDEYNIPDHTLLC 540
           QVFPNSWAAILLTFDNAGMWNLRSEL ENRYLGQQLY+SVLSPARSLRDEYNIPDHTLLC
Sbjct: 481 QVFPNSWAAILLTFDNAGMWNLRSELTENRYLGQQLYMSVLSPARSLRDEYNIPDHTLLC 540

Query: 541 GLVKGMPLPKPYTI 555
           GLVKGMPLPKPYTI
Sbjct: 541 GLVKGMPLPKPYTI 553

BLAST of Lsi10G009040 vs. ExPASy TrEMBL
Match: A0A5D3DE75 (L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G001200 PE=3 SV=1)

HSP 1 Score: 1111.3 bits (2873), Expect = 0.0e+00
Identity = 524/554 (94.58%), Postives = 539/554 (97.29%), Query Frame = 0

Query: 1   MGTMMFIVLLWFSVATIMSTVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNI 60
           M TMMF VLL  S AT MS VRGEDPYFFFTWN+TYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 2   MATMMFTVLLCLSAAT-MSMVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 61

Query: 61  NSTSNNNIVINVFNYLNEPFLLHWSGIQHRKNSWQDGLPGTSCPIPPGANFTYHFQVKDQ 120
           NSTSNNNIV+NVFN LNEPFLLHWSGIQHRKNSWQDGL GT+CPIPPGANFTYHFQVKDQ
Sbjct: 62  NSTSNNNIVVNVFNNLNEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGANFTYHFQVKDQ 121

Query: 121 IGSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQIL 180
           IGSFFYYPS AMHRAAGGFGGLR+NSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQIL
Sbjct: 122 IGSFFYYPSIAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQIL 181

Query: 181 DNGHRSIARPEGVLINGKIAKGDGTDEPLFTMKPGKTYKYRVCNVGLKTSLNFRFQGHTM 240
           DNGHRSIARPEGVLINGK AKGDGTDEPLFTMKPGKTYKYRVCNVGLKTSLNFRFQGHTM
Sbjct: 182 DNGHRSIARPEGVLINGKTAKGDGTDEPLFTMKPGKTYKYRVCNVGLKTSLNFRFQGHTM 241

Query: 241 KLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTADKEPRDYYMVASTRFIKNVVIGKGIVRY 300
           KLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTA++EP+DYYMVASTRFIKNV+IGKGI+RY
Sbjct: 242 KLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTANQEPKDYYMVASTRFIKNVIIGKGIIRY 301

Query: 301 TNGKGPASPEIPKAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIRLFN 360
           TNGKGPASPEIPKAP+GWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTI+LFN
Sbjct: 302 TNGKGPASPEIPKAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLFN 361

Query: 361 SATKVDGKLRYAINGVSHVDPETPLKLAEYFGIADKVFKYDTISDEGPAEGATTMTIAPN 420
           SATKVDGKLRYAINGVSHVDPETPLKLAEYFG+ADKVFKYD ISDEGP EGAT +TIAPN
Sbjct: 362 SATKVDGKLRYAINGVSHVDPETPLKLAEYFGMADKVFKYDIISDEGPTEGATAVTIAPN 421

Query: 421 VVNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRSNYNLLDAVSRHTI 480
           VVNATFRNF+EIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRSNYNLLDAVSRHTI
Sbjct: 422 VVNATFRNFIEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRSNYNLLDAVSRHTI 481

Query: 481 QVFPNSWAAILLTFDNAGMWNLRSELIENRYLGQQLYISVLSPARSLRDEYNIPDHTLLC 540
           QVFPNSWAAILLTFDNAGMWNLRSEL ENRYLGQQLY+SVLSPARSLRDEYNIPDHTLLC
Sbjct: 482 QVFPNSWAAILLTFDNAGMWNLRSELTENRYLGQQLYMSVLSPARSLRDEYNIPDHTLLC 541

Query: 541 GLVKGMPLPKPYTI 555
           GLVKGMPLPKPYTI
Sbjct: 542 GLVKGMPLPKPYTI 554

BLAST of Lsi10G009040 vs. ExPASy TrEMBL
Match: A0A5A7TDG2 (L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold366G001010 PE=3 SV=1)

HSP 1 Score: 1071.2 bits (2769), Expect = 1.4e-309
Identity = 505/554 (91.16%), Postives = 532/554 (96.03%), Query Frame = 0

Query: 1   MGTMMFIVLLWFSVATIMSTVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNI 60
           M  +MF  LL  S A +M+TVRGEDPYFFFTWN+TYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 1   MARLMFTALLCLSAAAMMATVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTSNNNIVINVFNYLNEPFLLHWSGIQHRKNSWQDGLPGTSCPIPPGANFTYHFQVKDQ 120
           NSTSNNN+VINVFN L+EPFLLHWSGIQHRKNSWQDGL GT+CPI PG NFTYHFQVKDQ
Sbjct: 61  NSTSNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIQPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQIL 180
           IGSFFYYPSTAMHRAAGGFGGLR+NSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQ L
Sbjct: 121 IGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQYL 180

Query: 181 DNGHRSIARPEGVLINGKIAKGDGTDEPLFTMKPGKTYKYRVCNVGLKTSLNFRFQGHTM 240
           D+G RSIARP+GVLINGK AKGDGTDEPLFTMKPGKTYKYRVCNVGLK+SLNFRFQGHTM
Sbjct: 181 DSG-RSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRVCNVGLKSSLNFRFQGHTM 240

Query: 241 KLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTADKEPRDYYMVASTRFIKNVVIGKGIVRY 300
           KLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTA++E +DYYMVASTRFIKNV++GKGIVRY
Sbjct: 241 KLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTANQESKDYYMVASTRFIKNVLVGKGIVRY 300

Query: 301 TNGKGPASPEIPKAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIRLFN 360
           T+GKGPASPEIP+APVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTI+L N
Sbjct: 301 TDGKGPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVN 360

Query: 361 SATKVDGKLRYAINGVSHVDPETPLKLAEYFGIADKVFKYDTISDEGPAEGATTMTIAPN 420
           SA+KVDGKLRYAINGVSHVDPETPLKLAEYFGIADKVFKYDTISDEG AEGATT+T+APN
Sbjct: 361 SASKVDGKLRYAINGVSHVDPETPLKLAEYFGIADKVFKYDTISDEGLAEGATTVTVAPN 420

Query: 421 VVNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRSNYNLLDAVSRHTI 480
           VVNATFR+F+EIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKR+NYNLLDAVSRHTI
Sbjct: 421 VVNATFRDFIEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRANYNLLDAVSRHTI 480

Query: 481 QVFPNSWAAILLTFDNAGMWNLRSELIENRYLGQQLYISVLSPARSLRDEYNIPDHTLLC 540
           QVFP SWAAILLTFDNAGMWNLRSEL ENRYLG QLYISVLSPARSLRDEYNIPD+TLLC
Sbjct: 481 QVFPKSWAAILLTFDNAGMWNLRSELTENRYLGSQLYISVLSPARSLRDEYNIPDNTLLC 540

Query: 541 GLVKGMPLPKPYTI 555
           GLVK MP+PKPYTI
Sbjct: 541 GLVKDMPMPKPYTI 553

BLAST of Lsi10G009040 vs. ExPASy TrEMBL
Match: A0A5D3DDZ2 (L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G001170 PE=3 SV=1)

HSP 1 Score: 1070.1 bits (2766), Expect = 3.1e-309
Identity = 504/554 (90.97%), Postives = 532/554 (96.03%), Query Frame = 0

Query: 1   MGTMMFIVLLWFSVATIMSTVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNI 60
           M  +MF  LL  S A +M+TV+GEDPYFFFTWN+TYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 1   MAGLMFTALLCLSAAAMMATVQGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTSNNNIVINVFNYLNEPFLLHWSGIQHRKNSWQDGLPGTSCPIPPGANFTYHFQVKDQ 120
           NSTSNNN+VINVFN L+EPFLLHWSGIQHRKN WQDGL GT+CPI PG NFTYHFQVKDQ
Sbjct: 61  NSTSNNNLVINVFNNLDEPFLLHWSGIQHRKNCWQDGLLGTNCPIQPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQIL 180
           IGSFFYYPSTAMHRAAGGFGGLR+NSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQ L
Sbjct: 121 IGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQYL 180

Query: 181 DNGHRSIARPEGVLINGKIAKGDGTDEPLFTMKPGKTYKYRVCNVGLKTSLNFRFQGHTM 240
           D+G RSIARP+GVLINGK AKGDGTDEPLFTMKPGKTYKYRVCNVGLK+SLNFRFQGHTM
Sbjct: 181 DSG-RSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRVCNVGLKSSLNFRFQGHTM 240

Query: 241 KLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTADKEPRDYYMVASTRFIKNVVIGKGIVRY 300
           KLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTA++E +DYYMVASTRFIKNV++GKGIVRY
Sbjct: 241 KLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTANQESKDYYMVASTRFIKNVLVGKGIVRY 300

Query: 301 TNGKGPASPEIPKAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIRLFN 360
           T+GKGPASPEIP+APVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTI+L N
Sbjct: 301 TDGKGPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVN 360

Query: 361 SATKVDGKLRYAINGVSHVDPETPLKLAEYFGIADKVFKYDTISDEGPAEGATTMTIAPN 420
           SA+KVDGKLRYAINGVSHVDPETPLKLAEYFGIADKVFKYDTISDEG AEGATT+T+APN
Sbjct: 361 SASKVDGKLRYAINGVSHVDPETPLKLAEYFGIADKVFKYDTISDEGLAEGATTVTVAPN 420

Query: 421 VVNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRSNYNLLDAVSRHTI 480
           VVNATFR+F+EIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKR+NYNLLDAVSRHTI
Sbjct: 421 VVNATFRDFIEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRANYNLLDAVSRHTI 480

Query: 481 QVFPNSWAAILLTFDNAGMWNLRSELIENRYLGQQLYISVLSPARSLRDEYNIPDHTLLC 540
           QVFP SWAAILLTFDNAGMWNLRSEL ENRYLG QLYISVLSPARSLRDEYNIPD+TLLC
Sbjct: 481 QVFPKSWAAILLTFDNAGMWNLRSELTENRYLGSQLYISVLSPARSLRDEYNIPDNTLLC 540

Query: 541 GLVKGMPLPKPYTI 555
           GLVKGMP+PKPYTI
Sbjct: 541 GLVKGMPMPKPYTI 553

BLAST of Lsi10G009040 vs. NCBI nr
Match: KAA0040127.1 (L-ascorbate oxidase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 1112.1 bits (2875), Expect = 0.0e+00
Identity = 524/554 (94.58%), Postives = 539/554 (97.29%), Query Frame = 0

Query: 1   MGTMMFIVLLWFSVATIMSTVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNI 60
           M TMMF VLL  S AT MS VRGEDPYFFFTWN+TYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 2   MATMMFTVLLCLSAAT-MSMVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 61

Query: 61  NSTSNNNIVINVFNYLNEPFLLHWSGIQHRKNSWQDGLPGTSCPIPPGANFTYHFQVKDQ 120
           NSTSNNNIV+NVFN LNEPFLLHWSGIQHRKNSWQDGL GT+CPIPPGANFTYHFQVKDQ
Sbjct: 62  NSTSNNNIVVNVFNNLNEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGANFTYHFQVKDQ 121

Query: 121 IGSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQIL 180
           IGSFFYYPS AMHRAAGGFGGLR+NSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQIL
Sbjct: 122 IGSFFYYPSIAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQIL 181

Query: 181 DNGHRSIARPEGVLINGKIAKGDGTDEPLFTMKPGKTYKYRVCNVGLKTSLNFRFQGHTM 240
           DNGHRSIARPEGVLINGK AKGDGTDEPLFTMKPGKTYKYRVCNVGLKTSLNFRFQGHTM
Sbjct: 182 DNGHRSIARPEGVLINGKTAKGDGTDEPLFTMKPGKTYKYRVCNVGLKTSLNFRFQGHTM 241

Query: 241 KLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTADKEPRDYYMVASTRFIKNVVIGKGIVRY 300
           KLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTA++EP+DYYMVASTRFIKNV+IGKGI+RY
Sbjct: 242 KLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTANQEPKDYYMVASTRFIKNVIIGKGIIRY 301

Query: 301 TNGKGPASPEIPKAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIRLFN 360
           TNGKGPASPEIPKAP+GWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTI+LFN
Sbjct: 302 TNGKGPASPEIPKAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLFN 361

Query: 361 SATKVDGKLRYAINGVSHVDPETPLKLAEYFGIADKVFKYDTISDEGPAEGATTMTIAPN 420
           SATKVDGKLRYAINGVSH+DPETPLKLAEYFGIADKVFKYD ISDEGP EGAT +TIAPN
Sbjct: 362 SATKVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDIISDEGPTEGATAVTIAPN 421

Query: 421 VVNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRSNYNLLDAVSRHTI 480
           VVNATFRNF+EIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRSNYNLLDAVSRHTI
Sbjct: 422 VVNATFRNFIEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRSNYNLLDAVSRHTI 481

Query: 481 QVFPNSWAAILLTFDNAGMWNLRSELIENRYLGQQLYISVLSPARSLRDEYNIPDHTLLC 540
           QVFPNSWAAILLTFDNAGMWNLRSEL ENRYLGQQLY+SVLSPARSLRDEYNIPDHTLLC
Sbjct: 482 QVFPNSWAAILLTFDNAGMWNLRSELTENRYLGQQLYMSVLSPARSLRDEYNIPDHTLLC 541

Query: 541 GLVKGMPLPKPYTI 555
           GLVKGMPLPKPYTI
Sbjct: 542 GLVKGMPLPKPYTI 554

BLAST of Lsi10G009040 vs. NCBI nr
Match: XP_008449912.2 (PREDICTED: L-ascorbate oxidase homolog [Cucumis melo])

HSP 1 Score: 1112.1 bits (2875), Expect = 0.0e+00
Identity = 524/554 (94.58%), Postives = 539/554 (97.29%), Query Frame = 0

Query: 1   MGTMMFIVLLWFSVATIMSTVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNI 60
           M TMMF VLL  S AT MS VRGEDPYFFFTWN+TYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 1   MATMMFTVLLCLSAAT-MSMVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTSNNNIVINVFNYLNEPFLLHWSGIQHRKNSWQDGLPGTSCPIPPGANFTYHFQVKDQ 120
           NSTSNNNIV+NVFN LNEPFLLHWSGIQHRKNSWQDGL GT+CPIPPGANFTYHFQVKDQ
Sbjct: 61  NSTSNNNIVVNVFNNLNEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGANFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQIL 180
           IGSFFYYPS AMHRAAGGFGGLR+NSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQIL
Sbjct: 121 IGSFFYYPSIAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQIL 180

Query: 181 DNGHRSIARPEGVLINGKIAKGDGTDEPLFTMKPGKTYKYRVCNVGLKTSLNFRFQGHTM 240
           DNGHRSIARPEGVLINGK AKGDGTDEPLFTMKPGKTYKYRVCNVGLKTSLNFRFQGHTM
Sbjct: 181 DNGHRSIARPEGVLINGKTAKGDGTDEPLFTMKPGKTYKYRVCNVGLKTSLNFRFQGHTM 240

Query: 241 KLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTADKEPRDYYMVASTRFIKNVVIGKGIVRY 300
           KLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTA++EP+DYYMVASTRFIKNV+IGKGI+RY
Sbjct: 241 KLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTANQEPKDYYMVASTRFIKNVIIGKGIIRY 300

Query: 301 TNGKGPASPEIPKAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIRLFN 360
           TNGKGPASPEIPKAP+GWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTI+LFN
Sbjct: 301 TNGKGPASPEIPKAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLFN 360

Query: 361 SATKVDGKLRYAINGVSHVDPETPLKLAEYFGIADKVFKYDTISDEGPAEGATTMTIAPN 420
           SATKVDGKLRYAINGVSH+DPETPLKLAEYFGIADKVFKYD ISDEGP EGAT +TIAPN
Sbjct: 361 SATKVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDIISDEGPTEGATAVTIAPN 420

Query: 421 VVNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRSNYNLLDAVSRHTI 480
           VVNATFRNF+EIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRSNYNLLDAVSRHTI
Sbjct: 421 VVNATFRNFIEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRSNYNLLDAVSRHTI 480

Query: 481 QVFPNSWAAILLTFDNAGMWNLRSELIENRYLGQQLYISVLSPARSLRDEYNIPDHTLLC 540
           QVFPNSWAAILLTFDNAGMWNLRSEL ENRYLGQQLY+SVLSPARSLRDEYNIPDHTLLC
Sbjct: 481 QVFPNSWAAILLTFDNAGMWNLRSELTENRYLGQQLYMSVLSPARSLRDEYNIPDHTLLC 540

Query: 541 GLVKGMPLPKPYTI 555
           GLVKGMPLPKPYTI
Sbjct: 541 GLVKGMPLPKPYTI 553

BLAST of Lsi10G009040 vs. NCBI nr
Match: TYK21748.1 (L-ascorbate oxidase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 1111.3 bits (2873), Expect = 0.0e+00
Identity = 524/554 (94.58%), Postives = 539/554 (97.29%), Query Frame = 0

Query: 1   MGTMMFIVLLWFSVATIMSTVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNI 60
           M TMMF VLL  S AT MS VRGEDPYFFFTWN+TYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 2   MATMMFTVLLCLSAAT-MSMVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 61

Query: 61  NSTSNNNIVINVFNYLNEPFLLHWSGIQHRKNSWQDGLPGTSCPIPPGANFTYHFQVKDQ 120
           NSTSNNNIV+NVFN LNEPFLLHWSGIQHRKNSWQDGL GT+CPIPPGANFTYHFQVKDQ
Sbjct: 62  NSTSNNNIVVNVFNNLNEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGANFTYHFQVKDQ 121

Query: 121 IGSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQIL 180
           IGSFFYYPS AMHRAAGGFGGLR+NSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQIL
Sbjct: 122 IGSFFYYPSIAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQIL 181

Query: 181 DNGHRSIARPEGVLINGKIAKGDGTDEPLFTMKPGKTYKYRVCNVGLKTSLNFRFQGHTM 240
           DNGHRSIARPEGVLINGK AKGDGTDEPLFTMKPGKTYKYRVCNVGLKTSLNFRFQGHTM
Sbjct: 182 DNGHRSIARPEGVLINGKTAKGDGTDEPLFTMKPGKTYKYRVCNVGLKTSLNFRFQGHTM 241

Query: 241 KLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTADKEPRDYYMVASTRFIKNVVIGKGIVRY 300
           KLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTA++EP+DYYMVASTRFIKNV+IGKGI+RY
Sbjct: 242 KLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTANQEPKDYYMVASTRFIKNVIIGKGIIRY 301

Query: 301 TNGKGPASPEIPKAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIRLFN 360
           TNGKGPASPEIPKAP+GWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTI+LFN
Sbjct: 302 TNGKGPASPEIPKAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLFN 361

Query: 361 SATKVDGKLRYAINGVSHVDPETPLKLAEYFGIADKVFKYDTISDEGPAEGATTMTIAPN 420
           SATKVDGKLRYAINGVSHVDPETPLKLAEYFG+ADKVFKYD ISDEGP EGAT +TIAPN
Sbjct: 362 SATKVDGKLRYAINGVSHVDPETPLKLAEYFGMADKVFKYDIISDEGPTEGATAVTIAPN 421

Query: 421 VVNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRSNYNLLDAVSRHTI 480
           VVNATFRNF+EIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRSNYNLLDAVSRHTI
Sbjct: 422 VVNATFRNFIEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRSNYNLLDAVSRHTI 481

Query: 481 QVFPNSWAAILLTFDNAGMWNLRSELIENRYLGQQLYISVLSPARSLRDEYNIPDHTLLC 540
           QVFPNSWAAILLTFDNAGMWNLRSEL ENRYLGQQLY+SVLSPARSLRDEYNIPDHTLLC
Sbjct: 482 QVFPNSWAAILLTFDNAGMWNLRSELTENRYLGQQLYMSVLSPARSLRDEYNIPDHTLLC 541

Query: 541 GLVKGMPLPKPYTI 555
           GLVKGMPLPKPYTI
Sbjct: 542 GLVKGMPLPKPYTI 554

BLAST of Lsi10G009040 vs. NCBI nr
Match: XP_038902254.1 (L-ascorbate oxidase homolog [Benincasa hispida])

HSP 1 Score: 1099.0 bits (2841), Expect = 0.0e+00
Identity = 521/554 (94.04%), Postives = 540/554 (97.47%), Query Frame = 0

Query: 1   MGTMMFIVLLWFSVATIMSTVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNI 60
           M TMMF VLL  S AT MSTVRGEDPYFFFTWN+TYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 1   MATMMFTVLLCLSAAT-MSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTSNNNIVINVFNYLNEPFLLHWSGIQHRKNSWQDGLPGTSCPIPPGANFTYHFQVKDQ 120
           NSTSNNNIVINVFN LNEPFLLHWSGIQHRKNSWQDGLPGT+CPIPPGANFTYHFQVKDQ
Sbjct: 61  NSTSNNNIVINVFNNLNEPFLLHWSGIQHRKNSWQDGLPGTNCPIPPGANFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQIL 180
           IGSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVL+GDWYTKSHTTLKQIL
Sbjct: 121 IGSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLVGDWYTKSHTTLKQIL 180

Query: 181 DNGHRSIARPEGVLINGKIAKGDGTDEPLFTMKPGKTYKYRVCNVGLKTSLNFRFQGHTM 240
           DNGHRSIARPEGVLINGK AKGDGTDEPLFTMKPGKTYKYRVCNVGLKTSLNFRFQGHTM
Sbjct: 181 DNGHRSIARPEGVLINGKTAKGDGTDEPLFTMKPGKTYKYRVCNVGLKTSLNFRFQGHTM 240

Query: 241 KLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTADKEPRDYYMVASTRFIKNVVIGKGIVRY 300
           KLVEMEGSHTVQNDY+SLD+HVGQCFSVLVTA++EP+DYY+VASTRFIKN+VIGKGI+RY
Sbjct: 241 KLVEMEGSHTVQNDYESLDIHVGQCFSVLVTANQEPKDYYVVASTRFIKNIVIGKGIIRY 300

Query: 301 TNGKGPASPEIPKAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIRLFN 360
           TNGKGPASPEIPKAP+GWAWSLNQFRTFRWNLTASAARPNPQGSY YGSINITRTI+LFN
Sbjct: 301 TNGKGPASPEIPKAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYRYGSINITRTIKLFN 360

Query: 361 SATKVDGKLRYAINGVSHVDPETPLKLAEYFGIADKVFKYDTISDEGPAEGATTMTIAPN 420
           SA+KVDGKLRYAINGVSHVDPETPLKLAEYFGIA+KVFKYD ISDEGP EG TTMTIAPN
Sbjct: 361 SASKVDGKLRYAINGVSHVDPETPLKLAEYFGIAEKVFKYDMISDEGP-EG-TTMTIAPN 420

Query: 421 VVNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRSNYNLLDAVSRHTI 480
           V+NATFRNFVEIIFENHEKSLQSWHL+GYSFFAVAIEPGRWSPEKR+NYNLLDAVSRHTI
Sbjct: 421 VINATFRNFVEIIFENHEKSLQSWHLNGYSFFAVAIEPGRWSPEKRANYNLLDAVSRHTI 480

Query: 481 QVFPNSWAAILLTFDNAGMWNLRSELIENRYLGQQLYISVLSPARSLRDEYNIPDHTLLC 540
           QVFP+SWAAILLTFDNAGMWNLRSEL ENRYLGQQLYISVLSPARSLRDEYNIPDHTLLC
Sbjct: 481 QVFPHSWAAILLTFDNAGMWNLRSELTENRYLGQQLYISVLSPARSLRDEYNIPDHTLLC 540

Query: 541 GLVKGMPLPKPYTI 555
           GLV GMPLPKPYTI
Sbjct: 541 GLVNGMPLPKPYTI 551

BLAST of Lsi10G009040 vs. NCBI nr
Match: XP_011653509.2 (LOW QUALITY PROTEIN: L-ascorbate oxidase homolog [Cucumis sativus])

HSP 1 Score: 1099.0 bits (2841), Expect = 0.0e+00
Identity = 523/554 (94.40%), Postives = 534/554 (96.39%), Query Frame = 0

Query: 1   MGTMMFIVLLWFSVATIMSTVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNI 60
           M TMMF VLL  S AT MS VRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 1   MATMMFTVLLCLSAAT-MSMVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTSNNNIVINVFNYLNEPFLLHWSGIQHRKNSWQDGLPGTSCPIPPGANFTYHFQVKDQ 120
           NSTSNNNIVINVFN LNEPFLLHWSGIQHRKNSWQDGL GT+CPI PG NFTYHFQVKDQ
Sbjct: 61  NSTSNNNIVINVFNNLNEPFLLHWSGIQHRKNSWQDGLLGTNCPILPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQIL 180
           IGSFFYYP  AMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQIL
Sbjct: 121 IGSFFYYPPIAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQIL 180

Query: 181 DNGHRSIARPEGVLINGKIAKGDGTDEPLFTMKPGKTYKYRVCNVGLKTSLNFRFQGHTM 240
           DNGHRSIARPEGVLINGK AKGDGTDEPLFTMKP KTYKYRVCNVGLKTSLNFRFQGHTM
Sbjct: 181 DNGHRSIARPEGVLINGKTAKGDGTDEPLFTMKPEKTYKYRVCNVGLKTSLNFRFQGHTM 240

Query: 241 KLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTADKEPRDYYMVASTRFIKNVVIGKGIVRY 300
           KLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTA++EP+DYYMVASTRFIKNV+IGKGI+RY
Sbjct: 241 KLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTANQEPKDYYMVASTRFIKNVLIGKGIIRY 300

Query: 301 TNGKGPASPEIPKAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIRLFN 360
           TNGKGPASPEIPKAP+GWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTI+LFN
Sbjct: 301 TNGKGPASPEIPKAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLFN 360

Query: 361 SATKVDGKLRYAINGVSHVDPETPLKLAEYFGIADKVFKYDTISDEGPAEGATTMTIAPN 420
           SATKVDGKLRYAINGVSHVDPETPLKLAEYF IA+KVFKYD ISDEGP EGAT +TIAPN
Sbjct: 361 SATKVDGKLRYAINGVSHVDPETPLKLAEYFEIAEKVFKYDMISDEGPTEGATAVTIAPN 420

Query: 421 VVNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRSNYNLLDAVSRHTI 480
           VVNATFRNF+EIIFENHEKSLQSWHLDGYSFFAVAIEPGRW PEKRSNYNLLDAVSRHTI
Sbjct: 421 VVNATFRNFIEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWXPEKRSNYNLLDAVSRHTI 480

Query: 481 QVFPNSWAAILLTFDNAGMWNLRSELIENRYLGQQLYISVLSPARSLRDEYNIPDHTLLC 540
           QVFPNSWAAILLTFDNAGMWNLRSELIENRYLGQQLYISVLSPARSLRDEYNIPDHTLLC
Sbjct: 481 QVFPNSWAAILLTFDNAGMWNLRSELIENRYLGQQLYISVLSPARSLRDEYNIPDHTLLC 540

Query: 541 GLVKGMPLPKPYTI 555
           GLVKGMPLPKPYTI
Sbjct: 541 GLVKGMPLPKPYTI 553

BLAST of Lsi10G009040 vs. TAIR 10
Match: AT3G13390.1 (SKU5 similar 11 )

HSP 1 Score: 899.8 bits (2324), Expect = 1.1e-261
Identity = 407/551 (73.87%), Postives = 470/551 (85.30%), Query Frame = 0

Query: 2   GTMMFIVLLWFSVATIMSTVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNIN 61
           G  +    L+ + A  +  VR EDPYF   WN+TYGT+SPLGVPQQ ILINGQFPGPN+N
Sbjct: 3   GVKLLAACLYLAAAATV-VVRAEDPYFHHVWNVTYGTVSPLGVPQQVILINGQFPGPNVN 62

Query: 62  STSNNNIVINVFNYLNEPFLLHWSGIQHRKNSWQDGLPGTSCPIPPGANFTYHFQVKDQI 121
           STSNNN++INVFN L+EPFLL W+GIQHRKN WQDG PGT CPI PG N+TYHFQ KDQI
Sbjct: 63  STSNNNVIINVFNNLDEPFLLTWNGIQHRKNCWQDGTPGTMCPIMPGTNYTYHFQPKDQI 122

Query: 122 GSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQILD 181
           GS+FYYPSTAMHR+AGGFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHT LK+ LD
Sbjct: 123 GSYFYYPSTAMHRSAGGFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTQLKKFLD 182

Query: 182 NGHRSIARPEGVLINGKIAKGDGTDEPLFTMKPGKTYKYRVCNVGLKTSLNFRFQGHTMK 241
           +G R++ RP+G+LINGK  KGDG+D PLFT+KPGKTY+ R+CNVGLKTSLNFR Q H +K
Sbjct: 183 SG-RTLGRPDGILINGKSGKGDGSDAPLFTLKPGKTYRVRICNVGLKTSLNFRIQNHKLK 242

Query: 242 LVEMEGSHTVQNDYQSLDVHVGQCFSVLVTADKEPRDYYMVASTRFIKNVVIGKGIVRYT 301
           LVEMEGSH +QNDY SLDVHVGQC+  ++TA++E +DYYMVAS+RF+K+V+   G++RY 
Sbjct: 243 LVEMEGSHVLQNDYDSLDVHVGQCYGTILTANQEAKDYYMVASSRFLKSVITTTGLLRYE 302

Query: 302 NGKGPASPEIPKAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIRLFNS 361
            GKGPAS ++P  PVGWAWSLNQFR+FRWNLTASAARPNPQGSYHYG INITRTI+L N+
Sbjct: 303 GGKGPASSQLPPGPVGWAWSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVNT 362

Query: 362 ATKVDGKLRYAINGVSHVDPETPLKLAEYFGIADKVFKYDTISDEGPAEGATTMTIAPNV 421
             KVDGKLRYA+NGVSH DPETPLKLAEYFG+ADKVFKYD+I+D    E   ++ I PNV
Sbjct: 363 QGKVDGKLRYALNGVSHTDPETPLKLAEYFGVADKVFKYDSITDNPTPEQIKSIKIVPNV 422

Query: 422 VNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRSNYNLLDAVSRHTIQ 481
           +N T R F+E++FENHEKS+QSWHLDGYSFFAVA+EPG W+PEKR NYNLLDAVSRHT+Q
Sbjct: 423 LNITHRTFIEVVFENHEKSVQSWHLDGYSFFAVAVEPGTWTPEKRKNYNLLDAVSRHTVQ 482

Query: 482 VFPNSWAAILLTFDNAGMWNLRSELIENRYLGQQLYISVLSPARSLRDEYNIPDHTLLCG 541
           V+P  WAAILLTFDN GMWN+RSE  E RYLGQQLY SVLSP +SLRDEYN+P+ +L CG
Sbjct: 483 VYPKCWAAILLTFDNCGMWNVRSENSERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCG 542

Query: 542 LVKGMPLPKPY 553
           LVKG P P PY
Sbjct: 543 LVKGTPKPNPY 551

BLAST of Lsi10G009040 vs. TAIR 10
Match: AT1G55570.1 (SKU5 similar 12 )

HSP 1 Score: 897.9 bits (2319), Expect = 4.1e-261
Identity = 409/551 (74.23%), Postives = 468/551 (84.94%), Query Frame = 0

Query: 2   GTMMFIVLLWFSVATIMSTVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNIN 61
           G  +  V L  + AT+M  V+ EDPYF   WN+TYGT SPLGVPQQ ILINGQFPGPNIN
Sbjct: 4   GVKLLAVCLCVATATVM-MVQAEDPYFHHVWNVTYGTASPLGVPQQVILINGQFPGPNIN 63

Query: 62  STSNNNIVINVFNYLNEPFLLHWSGIQHRKNSWQDGLPGTSCPIPPGANFTYHFQVKDQI 121
           STSNNN+++NVFN L+EPFL+ W+GIQHRKN WQDG  GT CPIPPG NFTYHFQ KDQI
Sbjct: 64  STSNNNVIVNVFNNLDEPFLITWAGIQHRKNCWQDGTAGTMCPIPPGQNFTYHFQPKDQI 123

Query: 122 GSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQILD 181
           GS+FYYP+TAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYT+LI DWYTKSHT LK+ LD
Sbjct: 124 GSYFYYPTTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTILINDWYTKSHTQLKKFLD 183

Query: 182 NGHRSIARPEGVLINGKIAKGDGTDEPLFTMKPGKTYKYRVCNVGLKTSLNFRFQGHTMK 241
           +G R+I RP+G+LINGK  K DG+D+PLFT+KPGKTY+ R+CNVGLK SLNFR Q H MK
Sbjct: 184 SG-RTIGRPDGILINGKSGKTDGSDKPLFTLKPGKTYRVRICNVGLKASLNFRIQNHKMK 243

Query: 242 LVEMEGSHTVQNDYQSLDVHVGQCFSVLVTADKEPRDYYMVASTRFIKNVVIGKGIVRYT 301
           LVEMEGSH +QNDY SLDVHVGQCF V+VTAD+EP+DYYM+ASTRF+K  +   G++RY 
Sbjct: 244 LVEMEGSHVLQNDYDSLDVHVGQCFGVIVTADQEPKDYYMIASTRFLKKPLTTTGLLRYE 303

Query: 302 NGKGPASPEIPKAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIRLFNS 361
            GKGPAS ++P APVGWAWSLNQ+R+FRWNLTASAARPNPQGSYHYG INITRTI+L N+
Sbjct: 304 GGKGPASSQLPAAPVGWAWSLNQYRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVNT 363

Query: 362 ATKVDGKLRYAINGVSHVDPETPLKLAEYFGIADKVFKYDTISDEGPAEGATTMTIAPNV 421
             KVDGKLRYA++GVSH DPETPLKLAEYFG+ADKVFKYDTISD    +    + I PNV
Sbjct: 364 QGKVDGKLRYALSGVSHTDPETPLKLAEYFGVADKVFKYDTISDNPNPDQIKNIKIEPNV 423

Query: 422 VNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRSNYNLLDAVSRHTIQ 481
           +N T R F+E++FENHE+S+QSWHLDGYSFFAVA+EPG W+PEKR NYNLLDAVSRHT+Q
Sbjct: 424 LNITHRTFIEVVFENHERSVQSWHLDGYSFFAVAVEPGTWTPEKRKNYNLLDAVSRHTVQ 483

Query: 482 VFPNSWAAILLTFDNAGMWNLRSELIENRYLGQQLYISVLSPARSLRDEYNIPDHTLLCG 541
           V+P  WAAILLTFDN GMWN+RSE  E RYLGQQLY SVLSP +SLRDEYN+P+ +L CG
Sbjct: 484 VYPKCWAAILLTFDNCGMWNIRSENAERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCG 543

Query: 542 LVKGMPLPKPY 553
           LVKG P   PY
Sbjct: 544 LVKGKPKVNPY 552

BLAST of Lsi10G009040 vs. TAIR 10
Match: AT3G13400.1 (SKU5 similar 13 )

HSP 1 Score: 880.6 bits (2274), Expect = 6.8e-256
Identity = 404/541 (74.68%), Postives = 466/541 (86.14%), Query Frame = 0

Query: 14  VATIMSTVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNINSTSNNNIVINVF 73
           +A+ ++ V   DPYF++TWN+TYGT +PLG+PQQ ILINGQFPGPN+NSTSNNN+VINVF
Sbjct: 13  LASTVALVSAGDPYFYYTWNVTYGTAAPLGIPQQVILINGQFPGPNLNSTSNNNVVINVF 72

Query: 74  NYLNEPFLLHWSGIQHRKNSWQDGLPGTSCPIPPGANFTYHFQVKDQIGSFFYYPSTAMH 133
           N L+EPFLL WSG+QHRKNSWQDG+ GTSCPIP G NFTYHFQ KDQIGS+FYYPSTA+H
Sbjct: 73  NNLDEPFLLTWSGLQHRKNSWQDGVTGTSCPIPAGTNFTYHFQPKDQIGSYFYYPSTALH 132

Query: 134 RAAGGFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQILDNGHRSIARPEGV 193
           R AGGFGGLRVNSRLLIPVPYADPEDD T+LI DWY KSHT LK  LD+G R++  P+GV
Sbjct: 133 RFAGGFGGLRVNSRLLIPVPYADPEDDRTILINDWYAKSHTALKNFLDSG-RTLGSPDGV 192

Query: 194 LINGKIAKGDGTDEPLFTMKPGKTYKYRVCNVGLKTSLNFRFQGHTMKLVEMEGSHTVQN 253
           LINGK  K  G + PLFTMKPGKTYKYR+CNVG K++LNFR QGH MKLVEMEGSH +QN
Sbjct: 193 LINGKSGKLGGNNAPLFTMKPGKTYKYRICNVGFKSTLNFRIQGHKMKLVEMEGSHVLQN 252

Query: 254 DYQSLDVHVGQCFSVLVTADKEPRDYYMVASTRFIKNVVIGKGIVRYTNGKGPASPEIPK 313
           DY SLDVHVGQCF+VLVTAD+  ++YYMVASTRF+K  V   G++ Y      AS +IPK
Sbjct: 253 DYDSLDVHVGQCFAVLVTADQVAKNYYMVASTRFLKKEVSTVGVMSYEGSNVQASSDIPK 312

Query: 314 APVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIRLFNSATKVDGKLRYAI 373
           APVGWAWSLNQFR+FRWNLTASAARPNPQGSYHYG INITRTI+L N+   V+GK+R+  
Sbjct: 313 APVGWAWSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLANTKNLVNGKVRFGF 372

Query: 374 NGVSHVDPETPLKLAEYFGIADKVFKYDTISDEGPAEGATTMTIAPNVVNATFRNFVEII 433
           NGVSHVD ETPLKLAEYFG+++KVFKY+ I DE PA   TT+T+ PNV+N TFR FVE++
Sbjct: 373 NGVSHVDTETPLKLAEYFGMSEKVFKYNVIKDE-PAAKITTLTVEPNVLNITFRTFVEVV 432

Query: 434 FENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRSNYNLLDAVSRHTIQVFPNSWAAILLT 493
           FENHEKS+QS+HLDGYSFFAVA EPGRW+PEKR+NYNLLDAVSRHT+QV+P SW+AILLT
Sbjct: 433 FENHEKSMQSFHLDGYSFFAVASEPGRWTPEKRNNYNLLDAVSRHTVQVYPKSWSAILLT 492

Query: 494 FDNAGMWNLRSELIENRYLGQQLYISVLSPARSLRDEYNIPDHTLLCGLVKGMPLPKPYT 553
           FDNAGMWN+RSE  E RYLGQQLY+SVLSP +SLRDEYNIP +T LCG+VKG+PLP PYT
Sbjct: 493 FDNAGMWNIRSENWERRYLGQQLYVSVLSPEKSLRDEYNIPLNTNLCGIVKGLPLPTPYT 551

Query: 554 I 555
           I
Sbjct: 553 I 551

BLAST of Lsi10G009040 vs. TAIR 10
Match: AT1G55560.1 (SKU5 similar 14 )

HSP 1 Score: 870.2 bits (2247), Expect = 9.2e-253
Identity = 398/540 (73.70%), Postives = 462/540 (85.56%), Query Frame = 0

Query: 14  VATIMSTVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNINSTSNNNIVINVF 73
           + + ++ V   DPYFF TWN+TYGT SPLGVPQ+ ILINGQFPGPN+NSTSNNN+VINVF
Sbjct: 12  LVSTVAIVNAGDPYFFHTWNVTYGTASPLGVPQKVILINGQFPGPNLNSTSNNNVVINVF 71

Query: 74  NYLNEPFLLHWSGIQHRKNSWQDGLPGTSCPIPPGANFTYHFQVKDQIGSFFYYPSTAMH 133
           N+L+EPFLL WSGIQHRKN WQDG+ GTSCPIP G NFTYHFQ KDQIGS+FYYP+T++H
Sbjct: 72  NHLDEPFLLTWSGIQHRKNCWQDGVAGTSCPIPAGQNFTYHFQPKDQIGSYFYYPTTSLH 131

Query: 134 RAAGGFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQILDNGHRSIARPEGV 193
           R AGGFGGLRVNSRLLIPVPYADPEDDYTVL+GDWYT  HT LK  LD+G R++  P GV
Sbjct: 132 RFAGGFGGLRVNSRLLIPVPYADPEDDYTVLLGDWYTAGHTALKNFLDSG-RTLGLPNGV 191

Query: 194 LINGKIAKGDGTDEPLFTMKPGKTYKYRVCNVGLKTSLNFRFQGHTMKLVEMEGSHTVQN 253
           LINGK  K  G +EPLFTMKPGKTYKYR+CNVG K++LNFR Q H MKLVEMEGSH +QN
Sbjct: 192 LINGKSGKVGGKNEPLFTMKPGKTYKYRLCNVGFKSTLNFRIQNHKMKLVEMEGSHVIQN 251

Query: 254 DYQSLDVHVGQCFSVLVTADKEPRDYYMVASTRFIKNVVIGKGIVRYTNGKGPASPEIPK 313
           DY SLDVHVGQCFSVLVTA++  +DYYMVASTRF+K  +   G++RY      AS E+PK
Sbjct: 252 DYDSLDVHVGQCFSVLVTANQAAKDYYMVASTRFLKKELSTVGVIRYEGSNVQASTELPK 311

Query: 314 APVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIRLFNSATKVDGKLRYAI 373
           APVGWAWSLNQFR+FRWNLT++AARPNPQGSYHYG INITR+I+L NS + VDGK+R+  
Sbjct: 312 APVGWAWSLNQFRSFRWNLTSNAARPNPQGSYHYGKINITRSIKLVNSKSVVDGKVRFGF 371

Query: 374 NGVSHVDPETPLKLAEYFGIADKVFKYDTISDEGPAEGATTMTIAPNVVNATFRNFVEII 433
           NGVSHVD ETPLKLAEYF +++KVFKY+ I DE PA   T +T+ PNV+N TFR FVEII
Sbjct: 372 NGVSHVDTETPLKLAEYFQMSEKVFKYNVIKDE-PAAKITALTVQPNVLNITFRTFVEII 431

Query: 434 FENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRSNYNLLDAVSRHTIQVFPNSWAAILLT 493
           FENHEK++QS+HLDGYSFFAVA EPGRW+PEKR NYNLLDAVSRHT+QV+P SW+AILLT
Sbjct: 432 FENHEKTMQSFHLDGYSFFAVASEPGRWTPEKRENYNLLDAVSRHTVQVYPKSWSAILLT 491

Query: 494 FDNAGMWNLRSELIENRYLGQQLYISVLSPARSLRDEYNIPDHTLLCGLVKGMPLPKPYT 553
           FDNAGMWN+RSE +E +YLG+QLY+SVLSP +SLRDEYNIP +T LCG+VKG+PLP  Y+
Sbjct: 492 FDNAGMWNIRSENLERKYLGEQLYVSVLSPEKSLRDEYNIPLNTNLCGIVKGLPLPAHYS 549

BLAST of Lsi10G009040 vs. TAIR 10
Match: AT1G21860.1 (SKU5 similar 7 )

HSP 1 Score: 635.6 bits (1638), Expect = 3.8e-182
Identity = 306/541 (56.56%), Postives = 388/541 (71.72%), Query Frame = 0

Query: 1   MGTMMFIVLLWFSVATIMSTVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNI 60
           M T   I  L F    ++S    EDPY FF W++TYG ISPLGV QQGILING+FPGP+I
Sbjct: 6   MNTRAMITTLLF----LISLAFAEDPYRFFEWHVTYGNISPLGVAQQGILINGKFPGPDI 65

Query: 61  NSTSNNNIVINVFNYLNEPFLLHWSGIQHRKNSWQDGLPGTSCPIPPGANFTYHFQVKDQ 120
            S +N+N++INVFN+L+EPFLL W+GI++ KNS+QDG+ GT CPIPPG N+TY  QVKDQ
Sbjct: 66  ISITNDNLIINVFNHLDEPFLLSWNGIRNWKNSFQDGVYGTMCPIPPGKNYTYALQVKDQ 125

Query: 121 IGSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQIL 180
           IGSF+Y+PS   H+AAGGFGG+R++SR LIPVP+  P DDYT+L+GDWY  +H  LK  L
Sbjct: 126 IGSFYYFPSLGFHKAAGGFGGIRISSRALIPVPFPTPADDYTLLVGDWYKTNHKDLKAQL 185

Query: 181 DNGHRSIARPEGVLINGKIAKGDGTDEPLFTMKPGKTYKYRVCNVGLKTSLNFRFQGHTM 240
           DNG + +  P+G+LING+      +      ++PGKTY+ R+ NVGL+ SLNFR Q HTM
Sbjct: 186 DNGGK-LPLPDGILINGR------SSGATLNIEPGKTYRLRISNVGLQNSLNFRIQNHTM 245

Query: 241 KLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTADKEPRDYYMVASTRFIKNVVIGKGIVRY 300
           KLVE+EG +T+QN + SLDVHVGQ +SVL+TAD+  +DYY+V S+RF   ++   G++ Y
Sbjct: 246 KLVEVEGRYTIQNLFSSLDVHVGQSYSVLITADQPAKDYYVVVSSRFTSKILTTTGVLHY 305

Query: 301 TNGKGPASPEIPKAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIRLFN 360
           +N   P S  IP  P+  +WS NQ R  R NLTAS  RPNPQGSY YG INITRTIRL N
Sbjct: 306 SNSVAPVSGPIPDGPIKLSWSFNQARAIRTNLTASGPRPNPQGSYRYGVINITRTIRLAN 365

Query: 361 SATKVDGKLRYAINGVSHVDPETPLKLAEYFGIADKVFKYDTISDEGPAEGATTMTIAPN 420
           +   ++GK RYA+N  S    +TPLKL +YF I D V+K  +ISD+ P  GA   T   +
Sbjct: 366 NLGHIEGKQRYAVNSASFYPADTPLKLVDYFKI-DGVYKPGSISDQ-PTNGAIFPT--TS 425

Query: 421 VVNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRSNYNLLDAVSRHTI 480
           V+ A FR FVE+IFEN E  +QSWHLDGYSF+ V +E G+WSP  R  YNL DA+ R TI
Sbjct: 426 VMQADFRAFVEVIFENSEDIVQSWHLDGYSFYVVGMELGKWSPASRKVYNLNDAILRCTI 485

Query: 481 QVFPNSWAAILLTFDNAGMWNLRSELIENRYLGQQLYISVLSPARSLRDEYNIPDHTLLC 540
           QV+P SW AI +  DN GMWN+RSE+ E +YLGQQ Y+ V + + SLRDEY IP + LLC
Sbjct: 486 QVYPRSWTAIYIALDNVGMWNMRSEIWERQYLGQQFYMRVYTTSTSLRDEYLIPKNALLC 531

Query: 541 G 542
           G
Sbjct: 546 G 531

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q006246.0e-25773.37L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1[more]
P291626.0e-24169.06L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q9SU406.0e-15648.22Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=... [more]
Q9FHN64.4e-15146.18Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=... [more]
Q8VXX51.2e-14846.65Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=... [more]
Match NameE-valueIdentityDescription
A0A5A7TF080.0e+0094.58L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A1S3BNS20.0e+0094.58L-ascorbate oxidase homolog OS=Cucumis melo OX=3656 GN=LOC103491644 PE=3 SV=1[more]
A0A5D3DE750.0e+0094.58L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A5A7TDG21.4e-30991.16L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A5D3DDZ23.1e-30990.97L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
Match NameE-valueIdentityDescription
KAA0040127.10.0e+0094.58L-ascorbate oxidase-like protein [Cucumis melo var. makuwa][more]
XP_008449912.20.0e+0094.58PREDICTED: L-ascorbate oxidase homolog [Cucumis melo][more]
TYK21748.10.0e+0094.58L-ascorbate oxidase-like protein [Cucumis melo var. makuwa][more]
XP_038902254.10.0e+0094.04L-ascorbate oxidase homolog [Benincasa hispida][more]
XP_011653509.20.0e+0094.40LOW QUALITY PROTEIN: L-ascorbate oxidase homolog [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT3G13390.11.1e-26173.87SKU5 similar 11 [more]
AT1G55570.14.1e-26174.23SKU5 similar 12 [more]
AT3G13400.16.8e-25674.68SKU5 similar 13 [more]
AT1G55560.19.2e-25373.70SKU5 similar 14 [more]
AT1G21860.13.8e-18256.56SKU5 similar 7 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001117Multicopper oxidase, type 1PFAMPF00394Cu-oxidasecoord: 159..299
e-value: 4.8E-39
score: 134.0
IPR008972CupredoxinGENE3D2.60.40.420coord: 157..338
e-value: 3.8E-35
score: 123.8
IPR008972CupredoxinGENE3D2.60.40.420coord: 348..545
e-value: 1.1E-34
score: 121.9
IPR008972CupredoxinGENE3D2.60.40.420coord: 29..148
e-value: 2.1E-33
score: 117.1
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 24..174
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 350..540
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 156..339
IPR011706Multicopper oxidase, C-terminalPFAMPF07731Cu-oxidase_2coord: 383..522
e-value: 2.3E-24
score: 85.7
IPR011707Multicopper oxidase, N-termianlPFAMPF07732Cu-oxidase_3coord: 34..147
e-value: 8.7E-40
score: 135.5
NoneNo IPR availablePANTHERPTHR11709:SF246L-ASCORBATE OXIDASE-LIKE PROTEINcoord: 4..545
IPR045087Multicopper oxidasePANTHERPTHR11709MULTI-COPPER OXIDASEcoord: 4..545
IPR034271Ascorbate oxidase homologue, second cupredoxin domainCDDcd13872CuRO_2_AAO_like_1coord: 159..300
e-value: 2.10503E-82
score: 251.553

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi10G009040.1Lsi10G009040.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005507 copper ion binding
molecular_function GO:0016491 oxidoreductase activity