Lsi10G006890 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi10G006890
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	terpene synthase 10-like
Location	chr10: 9664196 .. 9667135 (+)
RNA-Seq Expression	Lsi10G006890
Synteny	Lsi10G006890

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTCTTCACCATGTTCTTGGTTCTTCCTTGGTGAGATCTTTCATCAAACAAGTTTCTTTTCATCCTAAACCTTCTTTTGTTCCTCGTATTGCTAAAATAAAGGGGACTACAAAAATATCGGAGGATGATCAGATTATCGTTCGACGATCTGCAAATTACCAACCTCCTATATGGAAATACGAGTTTGTCCAATCACTGACGAGTGAGTTTAAGGTATATAATATGAGTTGAAATTATGTATTAGTTGTTTAGGTGTACTTCGTTAGTAATTAGGTTTAGTTATAGAATTATTTTGTGACCTATTTCATGTGATTTTCAGGGAGAAACATGCTCAAGACGTTTCAATGAGCTAAAGAAAAAGGTTGAAGTCCTGATGAGACGAATAGTTGATGACCCATTAAAACAACTCGAGTTCATTGACATTTTGCAACGACTTGGAATATCATACCACTTTGAGAATGAGATTAAGGATGTCTTAGAAAGAATATACAACAAGAGTTATGAAGATGGCGATAGGTTGAATAAGAGTTCTCTTTATCAAACATCCCTTGAATTTAGACTCTTGAGACAACATGGATTCAATGTTTCAGAAGGTATTGATTCATTCTTTTTTATTAACGTCCATTATATTACACATAATCATTCAAAATATTATAATATAGACATCATTTATTTTCTTATACTTGTAATACACACACTATTTAATTTAATAAAAGTGAGTTACTATAATGAAGAGATCTCCACATCTCAACATTTCTTATAGTAGAAAATTTGAAATAAAATCTTATGAGATAAAACTATTACAACTTTTTGTTAAAGGAAAAGAGAATATATGAAGTTATGTATAAAAAAAAAAGAATTTATGTATATTAAGTTATAATGTACTAGAATTGATCTCTTCGAAAAAGTTCATCCTAACAATTTAATTGTAATTAATAGTATAAGTATCAATTATTTACGGGTGATTTTTAAATATAATAAAATAAGTCAATTTATTTATAAATATAACAAAATGTAGTGATAGACATTGGTATGTACTGATAGATGTCTATTCGTGCTTATCATTGTTTATTACTGATAGACAGTAACATTTTGCTATATTTAAAAATATTTTCAATAGTTTTGTCATTTAAAATAATTATTTATTATTTATTTTACAATTAAATAATTTTACACATTTTAAATTAAATCACATATAATTCAGTATATTTAACGTGATATTATAATCAATATTTATACTACTACTTAAAAAAATTTAAGTATTGACAGTTTGCAAATAATTTGTAAATTTATATGCACATGTTTTACAAATAAACAATGGTAATACTTTAATCAAATGAAAAACAAAATAATGCTATTTGTTAAATTACCAATTTAAGAGAGGTTGTGTACAAGTACAACTGCCAAAAGTGATTCGTTAAGATTTTACCTTTCAATAAAATAATGATTAAGAACTATAAAATGAATATAACATAATGCTCGTAATATCAATTACTTTTGAGAGAAGTTGGATTTCAAAATAATTAACATAACAAGATGAGCACACATTCCTTGAAACTAAGGGGTCATGAGCCCACCATACCCCAAATAATGATTGTTTATTCTTTTAGCAATTATTTGACTACTTAATTATAAAATAAAATAAAATAAAACAGGAGATTTTTAAAAATAAAAAAAGGAAATTATTTAAACAAAATAACAAAATTTTTAGANTAAAAATATATATGGATCATTTAATGTAATTACATATTACAGTTCTAGAACTCCGCCTAGTTTATGAACATGAGGTTTAATTTAGAAAAATCCATTTGATTAAAAATGGTTTAAATATGGCAAATTGCAAAAGAATGGATAATAAAATAATAAGAAATACAAAAAGCAATTTGAATTCACTATGTAGGCGGCCAATCAAAAGCAAAAAGAATACATTAATTAATCAAATTTTGTTTTTTAAAATAAATAATTAATTATATATATATATGTATGTATGTATGATTGGTCCTCCTTAGAAATTACACAATCAATCAAACAAGGCTTCAAATATACCTATCTTTCTCTCATATGGCTCTTCACCATGTTCTTGGTTCTTCCTTGGTGAGATCTTTCATCAAACAAGTTTCTTTTCATCCTAAACCTTCTTTTGTTCCTCGTATTGCTAAAATAAAGGGGACTACAAAAATATCGGAGGATGATCAGATTATCGTTCGACGATCTGCAAATTACCAACCTCCTATATGGAAATACGAGTTTGTCCAATCACTGACGAGTGAGTTTAAGGTATATAATATGAGTTGAAATTATGTATTAGTTGTTTAGGTGTACTTCGTTAGTAATTAGGTTTAGTTATAGAATTATTTTGTGACCTATTTCATGTGATTTTCAGGGAGAAACATGCTCAAGACGTTTCAATGAGCTAAAGAAAAAGGTTGAAGTCCTGATGAGACGAATAGTTGATGACCCATTAAAACAACTCGAGTTCATTGACATTTTGCAACGACTTGGAATATCATACCACTTTGAGAATGAGATTAAGGATGTCTTAGAAAGAATATACAACAAGAGTTATGAAGATGGCGATAGGTTGAATAAGAGTTCTCTTTATCAAACATCCCTTGAATTTAGACTCTTGAGACAACATGGATTCAATGTTTCAGAAGGTATTGATTCATTCTTTTTTATTAACGTCCATTATATTACACATAATCATTCAAAATATTATAATATAGACATCATTTATTTTCTTATACTTGTAATACACACACTATTTAATTTAATAAAAGTGAGTTACTATAATGAAGAGATCTCCACATCTCAACATTTCTTATAGTAGAAAATTTGAAATAAAATCTTATGAGATAAAACTATTACAACTTTTTGTTAAAGGAAAAGAGAATATATGAAGTTATGTATAAAAAAAAAAGAATTTATGTATATTAA

mRNA sequence

ATGGCTCTTCACCATGTTCTTGGTTCTTCCTTGGTGAGATCTTTCATCAAACAAGTTTCTTTTCATCCTAAACCTTCTTTTGTTCCTCGTATTGCTAAAATAAAGGGGACTACAAAAATATCGGAGGATGATCAGATTATCGTTCGACGATCTGCAAATTACCAACCTCCTATATGGAAATACGAGTTTGTCCAATCACTGACGAGTGAGTTTAAGGGAGAAACATGCTCAAGACGTTTCAATGAGCTAAAGAAAAAGGTTGAAGTCCTGATGAGACGAATAGTTGATGACCCATTAAAACAACTCGAGTTCATTGACATTTTGCAACGACTTGGAATATCATACCACTTTGAGAATGAGATTAAGGATGTCTTAGAAAGAATATACAACAAGAGTTATGAAGATGGCGATAGAAATTACACAATCAATCAAACAAGGCTTCAAATATACCTATCTTTCTCTCATATGGCTCTTCACCATGTTCTTGGTTCTTCCTTGGTGAGATCTTTCATCAAACAAGTTTCTTTTCATCCTAAACCTTCTTTTGTTCCTCGTATTGCTAAAATAAAGGGGACTACAAAAATATCGGAGGATGATCAGATTATCGTTCGACGATCTGCAAATTACCAACCTCCTATATGGAAATACGAGTTTGTCCAATCACTGACGAGTGAGTTTAAGGGAGAAACATGCTCAAGACGTTTCAATGAGCTAAAGAAAAAGGTTGAAGTCCTGATGAGACGAATAGTTGATGACCCATTAAAACAACTCGAGTTCATTGACATTTTGCAACGACTTGGAATATCATACCACTTTGAGAATGAGATTAAGGATGTCTTAGAAAGAATATACAACAAGAGTTATGAAGATGGCGATAGGTTGAATAAGAGTTCTCTTTATCAAACATCCCTTGAATTTAGACTCTTGAGACAACATGGATTCAATGTTTCAGAAGGTATTGATTCATTCTTTTTTATTAACGAAAAGAGAATATATGAAGTTATGTATAAAAAAAAAAGAATTTATGTATATTAA

Coding sequence (CDS)

ATGGCTCTTCACCATGTTCTTGGTTCTTCCTTGGTGAGATCTTTCATCAAACAAGTTTCTTTTCATCCTAAACCTTCTTTTGTTCCTCGTATTGCTAAAATAAAGGGGACTACAAAAATATCGGAGGATGATCAGATTATCGTTCGACGATCTGCAAATTACCAACCTCCTATATGGAAATACGAGTTTGTCCAATCACTGACGAGTGAGTTTAAGGGAGAAACATGCTCAAGACGTTTCAATGAGCTAAAGAAAAAGGTTGAAGTCCTGATGAGACGAATAGTTGATGACCCATTAAAACAACTCGAGTTCATTGACATTTTGCAACGACTTGGAATATCATACCACTTTGAGAATGAGATTAAGGATGTCTTAGAAAGAATATACAACAAGAGTTATGAAGATGGCGATAGAAATTACACAATCAATCAAACAAGGCTTCAAATATACCTATCTTTCTCTCATATGGCTCTTCACCATGTTCTTGGTTCTTCCTTGGTGAGATCTTTCATCAAACAAGTTTCTTTTCATCCTAAACCTTCTTTTGTTCCTCGTATTGCTAAAATAAAGGGGACTACAAAAATATCGGAGGATGATCAGATTATCGTTCGACGATCTGCAAATTACCAACCTCCTATATGGAAATACGAGTTTGTCCAATCACTGACGAGTGAGTTTAAGGGAGAAACATGCTCAAGACGTTTCAATGAGCTAAAGAAAAAGGTTGAAGTCCTGATGAGACGAATAGTTGATGACCCATTAAAACAACTCGAGTTCATTGACATTTTGCAACGACTTGGAATATCATACCACTTTGAGAATGAGATTAAGGATGTCTTAGAAAGAATATACAACAAGAGTTATGAAGATGGCGATAGGTTGAATAAGAGTTCTCTTTATCAAACATCCCTTGAATTTAGACTCTTGAGACAACATGGATTCAATGTTTCAGAAGGTATTGATTCATTCTTTTTTATTAACGAAAAGAGAATATATGAAGTTATGTATAAAAAAAAAAGAATTTATGTATATTAA

Protein sequence

MALHHVLGSSLVRSFIKQVSFHPKPSFVPRIAKIKGTTKISEDDQIIVRRSANYQPPIWKYEFVQSLTSEFKGETCSRRFNELKKKVEVLMRRIVDDPLKQLEFIDILQRLGISYHFENEIKDVLERIYNKSYEDGDRNYTINQTRLQIYLSFSHMALHHVLGSSLVRSFIKQVSFHPKPSFVPRIAKIKGTTKISEDDQIIVRRSANYQPPIWKYEFVQSLTSEFKGETCSRRFNELKKKVEVLMRRIVDDPLKQLEFIDILQRLGISYHFENEIKDVLERIYNKSYEDGDRLNKSSLYQTSLEFRLLRQHGFNVSEGIDSFFFINEKRIYEVMYKKKRIYVY

Homology

[Close]

BLAST of Lsi10G006890 vs. ExPASy Swiss-Prot
Match: Q93X23 (Myrcene synthase, chloroplastic OS=Quercus ilex OX=58334 PE=1 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 4.8e-31
Identity = 68/151 (45.03%), Postives = 106/151 (70.20%), Query Frame = 0


Query: 174 VSFHPKPSFVPRIAKIKGTTKISEDDQIIVRRSANYQPPIWKYEFVQSLTSEFKGETCSR 233
           V+   +  ++ R  +     K+S    I+ RRSANYQP IW +++++SL  E+ GETC+R
Sbjct: 28  VTSRTRNGYLARPVQCMVANKVSTSPDIL-RRSANYQPSIWNHDYIESLRIEYVGETCTR 87

Query: 234 RFNELKKKVEVLMRRIVDDPLKQLEFIDILQRLGISYHFENEIKDVLERIYNKSYEDGDR 293
           + N LK++V +++ ++V +PL+QLE I+ILQRLG+SYHFE EIK +L+ +YN  +  GD 
Sbjct: 88  QINVLKEQVRMMLHKVV-NPLEQLELIEILQRLGLSYHFEEEIKRILDGVYNNDH-GGDT 147

Query: 294 LNKSSLYQTSLEFRLLRQHGFNVSEGIDSFF 325
               +LY T+L+FRLLRQHG++VS+ + + F
Sbjct: 148 WKAENLYATALKFRLLRQHGYSVSQEVFNSF 175

[Close]

BLAST of Lsi10G006890 vs. ExPASy Swiss-Prot
Match: Q6PWU2 ((-)-alpha-terpineol synthase OS=Vitis vinifera OX=29760 PE=1 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 1.6e-29
Identity = 72/141 (51.06%), Postives = 99/141 (70.21%), Query Frame = 0


Query: 184 PRIAKIKGTTKISEDDQIIVRRSANYQPPIWKYEFVQSLTSEFKGETCSRRFNELKKKVE 243
           PR  K+K     S  ++IIVRR+ANY P IW Y++VQSL S++ GET +RR ++LK+ V+
Sbjct: 31  PRGIKVK--IGNSNCEEIIVRRTANYHPTIWDYDYVQSLRSDYVGETYTRRLDKLKRDVK 90

Query: 244 VLMRRIVDDPLKQLEFIDILQRLGISYHFENEIKDVLERIYNKSYEDGDRLNKSSLYQTS 303
            ++ + V  PL QLE ID+LQRLGI YHF++EIK +L  IYN+ Y   +   K  LY T+
Sbjct: 91  PMLGK-VKKPLDQLELIDVLQRLGIYYHFKDEIKRILNGIYNQ-YNRHEEWQKDDLYATA 150

Query: 304 LEFRLLRQHGFNVSEGIDSFF 325
           LEFRLLRQHG++V + + S F
Sbjct: 151 LEFRLLRQHGYDVPQDVFSRF 167

[Close]

BLAST of Lsi10G006890 vs. ExPASy Swiss-Prot
Match: A0A1C9J6A7 ((R)-limonene synthase 1, chloroplastic OS=Citrus sinensis OX=2711 PE=1 SV=2)

HSP 1 Score: 122.9 bits (307), Expect = 7.2e-27
Identity = 67/127 (52.76%), Postives = 89/127 (70.08%), Query Frame = 0


Query: 198 DDQIIVRRSANYQPPIWKYEFVQSLTSEFKGETCSRRFNELKKKVEVLMRRIVDDPLKQL 257
           D+  + RRSANYQP IW ++F+QSL S +  ET  RR  ELK KV+  ++  V +PL QL
Sbjct: 47  DNLTVDRRSANYQPSIWDHDFLQSLNSNYTDETYKRRAEELKGKVKTAIKD-VTEPLDQL 106

Query: 258 EFIDILQRLGISYHFENEIKDVLERIYNKSYEDGDRLNKSSLYQTSLEFRLLRQHGFNVS 317
           E ID LQRLG++YHFE EI+++L  I+N + +   R  K +LY TSLEFRLLRQHG+ VS
Sbjct: 107 ELIDNLQRLGLAYHFEPEIRNILRNIHNHNKDYNWR--KENLYATSLEFRLLRQHGYPVS 166

Query: 318 EGIDSFF 325
           + + S F
Sbjct: 167 QEVFSGF 170

[Close]

BLAST of Lsi10G006890 vs. ExPASy Swiss-Prot
Match: B9T536 (Terpene synthase 10 OS=Ricinus communis OX=3988 GN=TPS10 PE=2 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 4.7e-26
Identity = 60/126 (47.62%), Postives = 91/126 (72.22%), Query Frame = 0


Query: 199 DQIIVRRSANYQPPIWKYEFVQSLTSEFKGETCSRRFNELKKKVEVLMRRIVDDPLKQLE 258
           ++ I+RRSANY+PPIW ++FVQSL SEF GE   +R ++LK+ V++++ + +  P  Q E
Sbjct: 8   NETIIRRSANYRPPIWDFDFVQSLKSEFVGELNIKRIDKLKEDVKMMLNKTM-APSDQFE 67

Query: 259 FIDILQRLGISYHFENEIKDVLERIYNKSYEDGDRLNKSSLYQTSLEFRLLRQHGFNVSE 318
            ID LQRLG++YHF +EIK +++ IYN S+ + +   K  L+  +L+FRLLRQHG+N+S+
Sbjct: 68  LIDTLQRLGLAYHFGDEIKRIVKSIYN-SHRNDNTWMKEDLHTIALQFRLLRQHGYNISQ 127

Query: 319 GIDSFF 325
            I   F
Sbjct: 128 EIFDIF 131

[Close]

BLAST of Lsi10G006890 vs. ExPASy Swiss-Prot
Match: Q8L5K3 ((R)-limonene synthase 1, chloroplastic OS=Citrus limon OX=2708 PE=2 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 1.4e-25
Identity = 62/127 (48.82%), Postives = 89/127 (70.08%), Query Frame = 0


Query: 198 DDQIIVRRSANYQPPIWKYEFVQSLTSEFKGETCSRRFNELKKKVEVLMRRIVDDPLKQL 257
           D+  + RRSANYQP IW ++F+QSL S +  E   RR  EL+ KV++ ++ ++ +PL QL
Sbjct: 47  DNLTVDRRSANYQPSIWDHDFLQSLNSNYTDEAYKRRAEELRGKVKIAIKDVI-EPLDQL 106

Query: 258 EFIDILQRLGISYHFENEIKDVLERIYNKSYEDGDRLNKSSLYQTSLEFRLLRQHGFNVS 317
           E ID LQRLG+++ FE EI+++L  IYN + +   R  K +LY TSLEFRLLRQHG+ VS
Sbjct: 107 ELIDNLQRLGLAHRFETEIRNILNNIYNNNKDYNWR--KENLYATSLEFRLLRQHGYPVS 166

Query: 318 EGIDSFF 325
           + + + F
Sbjct: 167 QEVFNGF 170

[Close]

BLAST of Lsi10G006890 vs. ExPASy TrEMBL
Match: A0A0A0LM62 (Terpene_synth domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G299890 PE=4 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 3.6e-45
Identity = 104/184 (56.52%), Postives = 132/184 (71.74%), Query Frame = 0


Query: 156 MALHHVLGSSLVRSFIKQVSFHPKPSFVPRIAK-IKGTTKISEDDQIIVRRSANYQPPIW 215
           MALH    SS    FI++VS +  PSF+PRI K I  TT      Q IVRRS NYQPP W
Sbjct: 1   MALHQFPTSSQTSRFIERVSCNFNPSFIPRIVKVISKTTATRMCSQTIVRRSGNYQPPFW 60

Query: 216 KYEFVQSLTSEFKGETCSRRFNELKKKVEVLMRRIVDDPLKQLEFIDILQRLGISYHFEN 275
           K+EF+QSL SEF GE    RFNELK+++ +++ +I+DDPLKQLE ID LQRLGISYHFEN
Sbjct: 61  KHEFIQSLRSEFGGEIYVGRFNELKREIRLIINQIIDDPLKQLELIDTLQRLGISYHFEN 120

Query: 276 EIKDVLERIYNKSYEDGDRLNKSSLYQTSLEFRLLRQHGFNVSEGIDSFFFINEKRIYEV 335
           EIK+VL+  Y KSYE+ D    ++LY TSLEFRLLRQHGFN+S+ + + F+ +E + +  
Sbjct: 121 EIKNVLKTTYEKSYEN-DYWKNNNLYATSLEFRLLRQHGFNLSQDVFNNFYSSETKSFNT 180

Query: 336 -MYK 338
            MY+
Sbjct: 181 QMYQ 183

[Close]

BLAST of Lsi10G006890 vs. ExPASy TrEMBL
Match: A0A6J1I2T8 (terpene synthase 10-like OS=Cucurbita maxima OX=3661 GN=LOC111470415 PE=4 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 1.0e-44
Identity = 103/182 (56.59%), Postives = 129/182 (70.88%), Query Frame = 0


Query: 156 MALHHVLGSSLVRSFIKQVSFHPKPSFVPRIAKIKGTTKISEDDQIIVRRSANYQPPIWK 215
           MALH    +S    FIK+VSF    SFVP + K+ G  ++S   Q IVRRS NY+PPIWK
Sbjct: 1   MALHQFF-TSFTSLFIKRVSFDLNCSFVPPVLKVHGGVRMS--SQTIVRRSGNYKPPIWK 60

Query: 216 YEFVQSLTSEFKGETCSRRFNELKKKVEVLMRRIVDDPLKQLEFIDILQRLGISYHFENE 275
            EF++SL SEFKGE      NELK++++++M +I+DDPLKQLE ID LQRLGISYHFENE
Sbjct: 61  LEFIESLKSEFKGEMYVEHLNELKEEIDIIMNQIIDDPLKQLELIDTLQRLGISYHFENE 120

Query: 276 IKDVLERIYNKSYEDGDRLNKSSLYQTSLEFRLLRQHGFNVSEGIDSFFFINEKRIYEVM 335
           IKD+L+R Y KSYE  D   K++L+ TSLEFRLLRQHGFNVS+ + + F    K     +
Sbjct: 121 IKDMLKRTYEKSYESDD-WKKNNLHATSLEFRLLRQHGFNVSQEVFNNFKDEMKNFSTRL 178

Query: 336 YK 338
           YK
Sbjct: 181 YK 178

[Close]

BLAST of Lsi10G006890 vs. ExPASy TrEMBL
Match: A0A6J1EJW9 (terpene synthase 10-like OS=Cucurbita moschata OX=3662 GN=LOC111434993 PE=4 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 1.8e-44
Identity = 101/182 (55.49%), Postives = 127/182 (69.78%), Query Frame = 0


Query: 156 MALHHVLGSSLVRSFIKQVSFHPKPSFVPRIAKIKGTTKISEDDQIIVRRSANYQPPIWK 215
           MALH  L S     F+K+V F    SF+P   K+     +S  +Q IVRRS NYQPPIWK
Sbjct: 1   MALHPFLTSFTTDFFMKRVYFDLNSSFIPPTVKVNCVAIVS--NQTIVRRSGNYQPPIWK 60

Query: 216 YEFVQSLTSEFKGETCSRRFNELKKKVEVLMRRIVDDPLKQLEFIDILQRLGISYHFENE 275
           +E++QSL SEFKGE    RFNELKK+V+V++ +I+DDPLK  E ID LQRLGISYHFENE
Sbjct: 61  HEYIQSLNSEFKGEIYVERFNELKKEVDVMINQIIDDPLKNFELIDTLQRLGISYHFENE 120

Query: 276 IKDVLERIYNKSYEDGDRLNKSSLYQTSLEFRLLRQHGFNVSEGIDSFFFINEKRIYEVM 335
           IKD+L+R Y KSYE  D   K++LY TSLEFR+LRQHG NVS+ + + F    K   + +
Sbjct: 121 IKDLLKRTYEKSYESDD-WKKNNLYATSLEFRILRQHGLNVSQEVFNNFKDETKNFSKQL 179

Query: 336 YK 338
           Y+
Sbjct: 181 YE 179

[Close]

BLAST of Lsi10G006890 vs. ExPASy TrEMBL
Match: A0A6J1DKS9 (terpene synthase 10-like OS=Momordica charantia OX=3673 GN=LOC111022002 PE=4 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 3.4e-43
Identity = 100/169 (59.17%), Postives = 122/169 (72.19%), Query Frame = 0


Query: 156 MALHHVLGSSLVRSFIKQVSFHPKPSFVPRIAKIKGTTKISEDDQIIVRRSANYQPPIWK 215
           MALH  L SS +  F KQVSF  +PSFVPR+ K KG   +S  +  IVRRSAN+ P IWK
Sbjct: 1   MALHQFLSSSPMTHFTKQVSFSLEPSFVPRVVKAKGIATMS-SNTTIVRRSANFHPSIWK 60

Query: 216 YEFVQSLTSEFKGETCSRRFNELKKKVEVLMRRIVDDPLKQLEFIDILQRLGISYHFENE 275
            EF+QSL+SEF GET   RFN LKK+VE +M  I+DDPLKQL+ ID LQRLGISYHFENE
Sbjct: 61  NEFIQSLSSEFMGETYVDRFNMLKKEVEGIMNHIIDDPLKQLDLIDTLQRLGISYHFENE 120

Query: 276 IKDVLERIYNKSYEDGDRLNKSSLYQTSLEFRLLRQHGFNVSEGIDSFF 325
           IK +LER Y +S +  +   K  +Y T+LEFRL+RQHGF+VS  + + F
Sbjct: 121 IKGILERRY-RSDQGNNSCKKIDVYATALEFRLMRQHGFSVSPEVFNNF 167

[Close]

BLAST of Lsi10G006890 vs. ExPASy TrEMBL
Match: A0A6J1EMY1 (terpene synthase 10-like OS=Cucurbita moschata OX=3662 GN=LOC111434992 PE=4 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 3.4e-43
Identity = 98/165 (59.39%), Postives = 118/165 (71.52%), Query Frame = 0


Query: 156 MALHHVLGSSLVRSFIKQVSFHPKPSFVPRIAKIKGTTKISEDDQIIVRRSANYQPPIWK 215
           M LH    S     FIK+VSF    SFV    K+ G  ++S   Q IVRRS NY+PPIWK
Sbjct: 1   MTLHQPFTSFTTSLFIKRVSFDLNCSFVSPALKVHGGVRMS--SQTIVRRSGNYKPPIWK 60

Query: 216 YEFVQSLTSEFKGETCSRRFNELKKKVEVLMRRIVDDPLKQLEFIDILQRLGISYHFENE 275
            EF++SL SEFKGE    R NELK+++ ++M +I+DDPLKQLE ID LQRLGISYHFENE
Sbjct: 61  AEFIESLKSEFKGEIYVERLNELKEEIGIIMNQIIDDPLKQLELIDTLQRLGISYHFENE 120

Query: 276 IKDVLERIYNKSYEDGDRLNKSSLYQTSLEFRLLRQHGFNVSEGI 321
           IKDVL+R Y K YE GD   K+++Y TSLEFRLLRQHGF VS+ +
Sbjct: 121 IKDVLKRTYEKRYE-GDDWKKNNMYATSLEFRLLRQHGFKVSQEV 162

[Close]

BLAST of Lsi10G006890 vs. NCBI nr
Match: XP_023512342.1 (terpene synthase 10-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 193.4 bits (490), Expect = 3.3e-45
Identity = 102/165 (61.82%), Postives = 122/165 (73.94%), Query Frame = 0


Query: 156 MALHHVLGSSLVRSFIKQVSFHPKPSFVPRIAKIKGTTKISEDDQIIVRRSANYQPPIWK 215
           MALH    S     FIK+VSF    SFVP   K+ G+ ++S   Q IVRRS NY+PPIWK
Sbjct: 1   MALHQSFTSFTTSLFIKRVSFDLNCSFVPPALKVHGSVRMS--SQTIVRRSGNYKPPIWK 60

Query: 216 YEFVQSLTSEFKGETCSRRFNELKKKVEVLMRRIVDDPLKQLEFIDILQRLGISYHFENE 275
            EF++SL SEFKGE    R NELK+++ ++M +I+DDPLKQLE IDILQRLGISYHFENE
Sbjct: 61  PEFIESLKSEFKGEMYVERLNELKEEIGIIMSQIIDDPLKQLELIDILQRLGISYHFENE 120

Query: 276 IKDVLERIYNKSYEDGDRLNKSSLYQTSLEFRLLRQHGFNVSEGI 321
           IKDVL+R Y K YE GD   K++LY TSLEFRLLRQHGF VS+ +
Sbjct: 121 IKDVLKRTYEKRYE-GDDWKKNNLYATSLEFRLLRQHGFKVSQEV 162

[Close]

BLAST of Lsi10G006890 vs. NCBI nr
Match: XP_031736485.1 (terpene synthase 10 isoform X2 [Cucumis sativus])

HSP 1 Score: 192.2 bits (487), Expect = 7.4e-45
Identity = 104/184 (56.52%), Postives = 132/184 (71.74%), Query Frame = 0


Query: 156 MALHHVLGSSLVRSFIKQVSFHPKPSFVPRIAK-IKGTTKISEDDQIIVRRSANYQPPIW 215
           MALH    SS    FI++VS +  PSF+PRI K I  TT      Q IVRRS NYQPP W
Sbjct: 1   MALHQFPTSSQTSRFIERVSCNFNPSFIPRIVKVISKTTATRMCSQTIVRRSGNYQPPFW 60

Query: 216 KYEFVQSLTSEFKGETCSRRFNELKKKVEVLMRRIVDDPLKQLEFIDILQRLGISYHFEN 275
           K+EF+QSL SEF GE    RFNELK+++ +++ +I+DDPLKQLE ID LQRLGISYHFEN
Sbjct: 61  KHEFIQSLRSEFGGEIYVGRFNELKREIRLIINQIIDDPLKQLELIDTLQRLGISYHFEN 120

Query: 276 EIKDVLERIYNKSYEDGDRLNKSSLYQTSLEFRLLRQHGFNVSEGIDSFFFINEKRIYEV 335
           EIK+VL+  Y KSYE+ D    ++LY TSLEFRLLRQHGFN+S+ + + F+ +E + +  
Sbjct: 121 EIKNVLKTTYEKSYEN-DYWKNNNLYATSLEFRLLRQHGFNLSQDVFNNFYSSETKSFNT 180

Query: 336 -MYK 338
            MY+
Sbjct: 181 QMYQ 183

[Close]

BLAST of Lsi10G006890 vs. NCBI nr
Match: XP_011649391.2 (terpene synthase 10 isoform X1 [Cucumis sativus])

HSP 1 Score: 192.2 bits (487), Expect = 7.4e-45
Identity = 104/184 (56.52%), Postives = 132/184 (71.74%), Query Frame = 0


Query: 156 MALHHVLGSSLVRSFIKQVSFHPKPSFVPRIAK-IKGTTKISEDDQIIVRRSANYQPPIW 215
           MALH    SS    FI++VS +  PSF+PRI K I  TT      Q IVRRS NYQPP W
Sbjct: 1   MALHQFPTSSQTSRFIERVSCNFNPSFIPRIVKVISKTTATRMCSQTIVRRSGNYQPPFW 60

Query: 216 KYEFVQSLTSEFKGETCSRRFNELKKKVEVLMRRIVDDPLKQLEFIDILQRLGISYHFEN 275
           K+EF+QSL SEF GE    RFNELK+++ +++ +I+DDPLKQLE ID LQRLGISYHFEN
Sbjct: 61  KHEFIQSLRSEFGGEIYVGRFNELKREIRLIINQIIDDPLKQLELIDTLQRLGISYHFEN 120

Query: 276 EIKDVLERIYNKSYEDGDRLNKSSLYQTSLEFRLLRQHGFNVSEGIDSFFFINEKRIYEV 335
           EIK+VL+  Y KSYE+ D    ++LY TSLEFRLLRQHGFN+S+ + + F+ +E + +  
Sbjct: 121 EIKNVLKTTYEKSYEN-DYWKNNNLYATSLEFRLLRQHGFNLSQDVFNNFYSSETKSFNT 180

Query: 336 -MYK 338
            MY+
Sbjct: 181 QMYQ 183

[Close]

BLAST of Lsi10G006890 vs. NCBI nr
Match: XP_022971747.1 (terpene synthase 10-like [Cucurbita maxima])

HSP 1 Score: 190.7 bits (483), Expect = 2.2e-44
Identity = 103/182 (56.59%), Postives = 129/182 (70.88%), Query Frame = 0


Query: 156 MALHHVLGSSLVRSFIKQVSFHPKPSFVPRIAKIKGTTKISEDDQIIVRRSANYQPPIWK 215
           MALH    +S    FIK+VSF    SFVP + K+ G  ++S   Q IVRRS NY+PPIWK
Sbjct: 1   MALHQFF-TSFTSLFIKRVSFDLNCSFVPPVLKVHGGVRMS--SQTIVRRSGNYKPPIWK 60

Query: 216 YEFVQSLTSEFKGETCSRRFNELKKKVEVLMRRIVDDPLKQLEFIDILQRLGISYHFENE 275
            EF++SL SEFKGE      NELK++++++M +I+DDPLKQLE ID LQRLGISYHFENE
Sbjct: 61  LEFIESLKSEFKGEMYVEHLNELKEEIDIIMNQIIDDPLKQLELIDTLQRLGISYHFENE 120

Query: 276 IKDVLERIYNKSYEDGDRLNKSSLYQTSLEFRLLRQHGFNVSEGIDSFFFINEKRIYEVM 335
           IKD+L+R Y KSYE  D   K++L+ TSLEFRLLRQHGFNVS+ + + F    K     +
Sbjct: 121 IKDMLKRTYEKSYESDD-WKKNNLHATSLEFRLLRQHGFNVSQEVFNNFKDEMKNFSTRL 178

Query: 336 YK 338
           YK
Sbjct: 181 YK 178

[Close]

BLAST of Lsi10G006890 vs. NCBI nr
Match: XP_022928104.1 (terpene synthase 10-like [Cucurbita moschata])

HSP 1 Score: 189.9 bits (481), Expect = 3.7e-44
Identity = 101/182 (55.49%), Postives = 127/182 (69.78%), Query Frame = 0


Query: 156 MALHHVLGSSLVRSFIKQVSFHPKPSFVPRIAKIKGTTKISEDDQIIVRRSANYQPPIWK 215
           MALH  L S     F+K+V F    SF+P   K+     +S  +Q IVRRS NYQPPIWK
Sbjct: 1   MALHPFLTSFTTDFFMKRVYFDLNSSFIPPTVKVNCVAIVS--NQTIVRRSGNYQPPIWK 60

Query: 216 YEFVQSLTSEFKGETCSRRFNELKKKVEVLMRRIVDDPLKQLEFIDILQRLGISYHFENE 275
           +E++QSL SEFKGE    RFNELKK+V+V++ +I+DDPLK  E ID LQRLGISYHFENE
Sbjct: 61  HEYIQSLNSEFKGEIYVERFNELKKEVDVMINQIIDDPLKNFELIDTLQRLGISYHFENE 120

Query: 276 IKDVLERIYNKSYEDGDRLNKSSLYQTSLEFRLLRQHGFNVSEGIDSFFFINEKRIYEVM 335
           IKD+L+R Y KSYE  D   K++LY TSLEFR+LRQHG NVS+ + + F    K   + +
Sbjct: 121 IKDLLKRTYEKSYESDD-WKKNNLYATSLEFRILRQHGLNVSQEVFNNFKDETKNFSKQL 179

Query: 336 YK 338
           Y+
Sbjct: 181 YE 179

[Close]

BLAST of Lsi10G006890 vs. TAIR 10
Match: AT2G24210.1 (terpene synthase 10 )

HSP 1 Score: 101.7 bits (252), Expect = 1.2e-21
Identity = 57/126 (45.24%), Postives = 77/126 (61.11%), Query Frame = 0


Query: 202 IVRRSANYQPPIWKYEFVQSLTSEFKGETCSRRFNELKKKVEVLMRRIVDDPLKQLEFID 261
           + RRSANYQP  W +  + S+ ++F  +   R  + LK+KV  ++       L QLEFID
Sbjct: 44  VQRRSANYQPSRWDHHHLLSVENKFAKDKRVRERDLLKEKVRKMLNDEQKTYLDQLEFID 103

Query: 262 ILQRLGISYHFENEIKDVLERIYNKSYEDGDRLNKSSLYQTSLEFRLLRQHGFNVSEGID 321
            LQ+LG+SYHFE EI ++L   Y K   D   + +S L+ T+LEFRL RQHGFNVSE + 
Sbjct: 104 DLQKLGVSYHFEAEIDNILTSSYKK---DRTNIQESDLHATALEFRLFRQHGFNVSEDVF 163

Query: 322 SFFFIN 328
             F  N
Sbjct: 164 DVFMEN 166

[Close]

BLAST of Lsi10G006890 vs. TAIR 10
Match: AT4G16740.1 (terpene synthase 03 )

HSP 1 Score: 100.5 bits (249), Expect = 2.7e-21
Identity = 54/124 (43.55%), Postives = 79/124 (63.71%), Query Frame = 0


Query: 201 IIVRRSANYQPPIWKYEFVQSLTSEFKGETCSRRFNELKKKVEVLMRRIVDDPLKQLEFI 260
           ++ RRSANYQP +W++E++ SL + +  E    R   LK++V  ++    +  L+QLE I
Sbjct: 23  LVSRRSANYQPSLWQHEYLLSLGNTYVKEDNVERVTLLKQEVSKMLNE-TEGLLEQLELI 82

Query: 261 DILQRLGISYHFENEIKDVLERIYNKSYEDG----DRLNKSSLYQTSLEFRLLRQHGFNV 320
           D LQRLG+SYHFE EIK  L  ++ K+        DR     LY T+LEFRLLRQHGF++
Sbjct: 83  DTLQRLGVSYHFEQEIKKTLTNVHVKNVRAHKNRIDRNRWGDLYATALEFRLLRQHGFSI 142

[Close]

BLAST of Lsi10G006890 vs. TAIR 10
Match: AT4G16740.2 (terpene synthase 03 )

HSP 1 Score: 100.5 bits (249), Expect = 2.7e-21
Identity = 54/124 (43.55%), Postives = 79/124 (63.71%), Query Frame = 0


Query: 201 IIVRRSANYQPPIWKYEFVQSLTSEFKGETCSRRFNELKKKVEVLMRRIVDDPLKQLEFI 260
           ++ RRSANYQP +W++E++ SL + +  E    R   LK++V  ++    +  L+QLE I
Sbjct: 23  LVSRRSANYQPSLWQHEYLLSLGNTYVKEDNVERVTLLKQEVSKMLNE-TEGLLEQLELI 82

Query: 261 DILQRLGISYHFENEIKDVLERIYNKSYEDG----DRLNKSSLYQTSLEFRLLRQHGFNV 320
           D LQRLG+SYHFE EIK  L  ++ K+        DR     LY T+LEFRLLRQHGF++
Sbjct: 83  DTLQRLGVSYHFEQEIKKTLTNVHVKNVRAHKNRIDRNRWGDLYATALEFRLLRQHGFSI 142

[Close]

BLAST of Lsi10G006890 vs. TAIR 10
Match: AT3G25820.1 (terpene synthase-like sequence-1,8-cineole )

HSP 1 Score: 98.2 bits (243), Expect = 1.4e-20
Identity = 59/134 (44.03%), Postives = 83/134 (61.94%), Query Frame = 0


Query: 190 KGTTKISEDDQII--VRRSANYQPPIWKYEFVQSLTSEFKGETCSRRFNELKKKVEVLMR 249
           K  TK + D  ++   RRS NYQP  W + ++ S+ +++  ET     + LKKKV+ ++ 
Sbjct: 31  KSMTKTTPDTTLVELSRRSGNYQPSPWNHCYLLSIENKYASETEVITRDVLKKKVKSMLD 90

Query: 250 RIVDDPLKQLEFIDILQRLGISYHFENEIKDVLERIYNKSYEDGDRLNK-SSLYQTSLEF 309
                 L+QLE ID LQ+LG+SYHFE EI D L  ++ K   +  + +K   L+ TSLEF
Sbjct: 91  DEKKSRLEQLELIDDLQKLGVSYHFEIEINDTLTDLHLKMGRNCWKCDKEEDLHATSLEF 150

Query: 310 RLLRQHGFNVSEGI 321
           RLLRQHGF+VSE I
Sbjct: 151 RLLRQHGFDVSENI 164

[Close]

BLAST of Lsi10G006890 vs. TAIR 10
Match: AT3G25830.1 (terpene synthase-like sequence-1,8-cineole )

HSP 1 Score: 98.2 bits (243), Expect = 1.4e-20
Identity = 59/134 (44.03%), Postives = 83/134 (61.94%), Query Frame = 0


Query: 190 KGTTKISEDDQII--VRRSANYQPPIWKYEFVQSLTSEFKGETCSRRFNELKKKVEVLMR 249
           K  TK + D  ++   RRS NYQP  W + ++ S+ +++  ET     + LKKKV+ ++ 
Sbjct: 31  KSMTKTTPDTTLVELSRRSGNYQPSPWNHCYLLSIENKYASETEVITRDVLKKKVKSMLD 90

Query: 250 RIVDDPLKQLEFIDILQRLGISYHFENEIKDVLERIYNKSYEDGDRLNK-SSLYQTSLEF 309
                 L+QLE ID LQ+LG+SYHFE EI D L  ++ K   +  + +K   L+ TSLEF
Sbjct: 91  DEKKSRLEQLELIDDLQKLGVSYHFEIEINDTLTDLHLKMGRNCWKCDKEEDLHATSLEF 150

Query: 310 RLLRQHGFNVSEGI 321
           RLLRQHGF+VSE I
Sbjct: 151 RLLRQHGFDVSENI 164

The following BLAST results are available for this feature:

BLAST of Lsi10G006890 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93X23	4.8e-31	45.03	Myrcene synthase, chloroplastic OS=Quercus ilex OX=58334 PE=1 SV=1	[more]
Q6PWU2	1.6e-29	51.06	(-)-alpha-terpineol synthase OS=Vitis vinifera OX=29760 PE=1 SV=1	[more]
A0A1C9J6A7	7.2e-27	52.76	(R)-limonene synthase 1, chloroplastic OS=Citrus sinensis OX=2711 PE=1 SV=2	[more]
B9T536	4.7e-26	47.62	Terpene synthase 10 OS=Ricinus communis OX=3988 GN=TPS10 PE=2 SV=1	[more]
Q8L5K3	1.4e-25	48.82	(R)-limonene synthase 1, chloroplastic OS=Citrus limon OX=2708 PE=2 SV=1	[more]

BLAST of Lsi10G006890 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LM62	3.6e-45	56.52	Terpene_synth domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G2998...	[more]
A0A6J1I2T8	1.0e-44	56.59	terpene synthase 10-like OS=Cucurbita maxima OX=3661 GN=LOC111470415 PE=4 SV=1	[more]
A0A6J1EJW9	1.8e-44	55.49	terpene synthase 10-like OS=Cucurbita moschata OX=3662 GN=LOC111434993 PE=4 SV=1	[more]
A0A6J1DKS9	3.4e-43	59.17	terpene synthase 10-like OS=Momordica charantia OX=3673 GN=LOC111022002 PE=4 SV=...	[more]
A0A6J1EMY1	3.4e-43	59.39	terpene synthase 10-like OS=Cucurbita moschata OX=3662 GN=LOC111434992 PE=4 SV=1	[more]

BLAST of Lsi10G006890 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023512342.1	3.3e-45	61.82	terpene synthase 10-like [Cucurbita pepo subsp. pepo]	[more]
XP_031736485.1	7.4e-45	56.52	terpene synthase 10 isoform X2 [Cucumis sativus]	[more]
XP_011649391.2	7.4e-45	56.52	terpene synthase 10 isoform X1 [Cucumis sativus]	[more]
XP_022971747.1	2.2e-44	56.59	terpene synthase 10-like [Cucurbita maxima]	[more]
XP_022928104.1	3.7e-44	55.49	terpene synthase 10-like [Cucurbita moschata]	[more]

BLAST of Lsi10G006890 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G24210.1	1.2e-21	45.24	terpene synthase 10	[more]
AT4G16740.1	2.7e-21	43.55	terpene synthase 03	[more]
AT4G16740.2	2.7e-21	43.55	terpene synthase 03	[more]
AT3G25820.1	1.4e-20	44.03	terpene synthase-like sequence-1,8-cineole	[more]
AT3G25830.1	1.4e-20	44.03	terpene synthase-like sequence-1,8-cineole	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001906	Terpene synthase, N-terminal domain	PFAM	PF01397	Terpene_synth	coord: 213..319 e-value: 5.8E-28 score: 97.9 coord: 58..137 e-value: 1.8E-16 score: 60.4
IPR036965	Terpene synthase, N-terminal domain superfamily	GENE3D	1.50.10.130		coord: 214..340 e-value: 1.4E-33 score: 118.1 coord: 59..175 e-value: 4.6E-22 score: 80.3
None	No IPR available	PANTHER	PTHR31225	OS04G0344100 PROTEIN-RELATED	coord: 24..156
None	No IPR available	PANTHER	PTHR31225:SF114	TERPENE SYNTHASE 10-LIKE	coord: 179..324
None	No IPR available	PANTHER	PTHR31225:SF114	TERPENE SYNTHASE 10-LIKE	coord: 24..156
None	No IPR available	PANTHER	PTHR31225	OS04G0344100 PROTEIN-RELATED	coord: 179..324
IPR008930	Terpenoid cyclases/protein prenyltransferase alpha-alpha toroid	SUPERFAMILY	48239	Terpenoid cyclases/Protein prenyltransferases	coord: 210..328
IPR008930	Terpenoid cyclases/protein prenyltransferase alpha-alpha toroid	SUPERFAMILY	48239	Terpenoid cyclases/Protein prenyltransferases	coord: 55..141

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi10G006890.1	Lsi10G006890.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016114	terpenoid biosynthetic process
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0010333	terpene synthase activity
molecular_function	GO:0016829	lyase activity