Lsi10G006640 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi10G006640
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Shikimate kinase
Location	chr10: 9237032 .. 9239256 (-)
RNA-Seq Expression	Lsi10G006640
Synteny	Lsi10G006640

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATCTTGATCGGGCTGCTCTTTTTCTCTTCAATAATGAGGATATTTCAACTGTTTGTCTCCCTAGTACAATTGAATTTGTACTTCAGAGTACTCTTAAAGTGTTATATATAGTTTCTAGAATTGATCAAAATGATCTTATCATATTTCATGATTCGAGATTTTCACTTTGTTTAGTTTAGGGGATTGGGTTCTTGCTTAGAATAAAACCTCCTATTAGCCTCGAAGGTAAAAGTTAATATCATGCTAATAATGCTGTATGATAAATAGTAGTTACTTTGTTTTTTTATTCTTCCTTCTTAAAGATAATATTATAATACAGTGAAAAGGTTCATTATCACTTGTGAAAAATAATTTCAAACTGTTCACTGGGCCATTATATTTTGCCATAGACTTGGAACGTGTAACGTTTCGAATTTCTCACTCTTAAAATATTGATAGCGCAAGGCAAGCTCTATTTTATGCATATATGAACTCCAAAACAGAATCTTAGCTGATTAACATACCGGTGAACCTAACTTATCTTGATTCCTGCAGACGGAGGCATTGAGGAAGTTATCTTTGATGCGTCAGTTTGTTATTTCCACTGGTGGAGGTGCCGTTACGAGAACGATAAACTGGTAAAGAAGATAGACTAGTTGGATACAAGTGCTACATTTTTCCAGCTGAACTTAACTTTCAATATCATCTGACTGAACCTTTATTTTTAACTTTCCAGGAAATATATGCATAAAGGGATCAGTGTCTGGTTGGATGTGCCTTTGGAAGCCTTGGTTAAGAGAATTTCAGCTGTAGGAACCAATTCACGCCCCCTTTTGCATCATGATTCAACTGATGTATACTCAAAGGTATCTGTTTTCAGTGTTTGTTTCCCTTGACTCATAAATATGATTTTCCAGGTTTCTGATGTTGAAGTTCTTTTACATGAAACATACACAGAGTGTATGATTTCAAAGTTTCTGTTTTCTCGTGACAACTTGAGAAATAATAATCCTTGAAGATTAGTTTTAAAATTGACTCTCGAATAATATTTGATTCCTGTTATCTACAGACCCTCGTCCGTTTATCTACTCTCCTGGAAGAGAGGGGTGAAGCATATGCCAACGCTGAAGTCAAAGTTTCCTGCGAAAGTAAGACATCTCTCCTTGTTAGTTTATTCATGTATTCTACCAGGGGATGTACAAATAAGAACTTTTAAAATAATGGCAGCTTAATGTCAAAAACTCAATGGTTTTGGTTTTTATTCTCAAATTATAATCTCATAAGACTACCGACCACTAAAAGGATGCCATTTAGTTTAGGATGAGTTTGAAATGAATTTTAGAGGGTAAATTTTAACTTTGACTGCTTTAGCACATACACTTCTTAGTATTTCTTTGATACAGAAGATTGCTTTCATTTAAAGCTCTTTTTTCAAATGCCATTCCAAACAATATGTATTATTTGGTTGTTCCTCTATGCTAAGAATCTAAAATCTCAATATCAAATGACATAAGCTGAATGTTTAACTTGTGCAGAAATTGCAGCCAAACTGGGTACTAAAGACGTATCTAATGTCACACCTATGGCCATAGCAATCGAGGTACGGTCTATCTCTCTCCGTCTTTGAATAAATATGATAATTTACACTACAATTAGCTAAAGCCTTTTAGATAACGAGTCTTATTTTATTTCTATAACAATATTTTGCCTCTTGTTTATATTATGGAATCTCAGGCACTCGAACAAATCGAGATCTTTTTGAAGAGAGAAGATGGTTATTGTGCATTTTGATGGCCACAGGAAATGACTCATTTAGGTATAATAGCAGCAATGAAATGAAGTAGATGATGTTTTCATTTCATTTTTCCTTATGGAGTCGTGTATTTTTCTTATGATTTCAGGTACATATGAATAAAGGCAATGGGTGGAACAAATTTGATTTTTTCATTATTTCTGGATAATCTTCTCAATCAAGTATCAAGGCATGTTTCAGTTTCACTCAAGGCTGTCCCATATTTATTGATCTCAGTAAGTGTGTTTGATGTAGTGTAGTGTATCTACAATGTATCATCTGAAAAATGTGGATGGTTATAAGTTTCCAAGCATATGGAAAAACTAGCCATTAGTTGTATTTGAAAGAGTTGGTGAATTCATCTAAGTTAAGAACTTGATGATATCATTCAATTAAGGTTATATTATTGAAATACCCCTAAATTAGGTTCTATTGCATTAGATTTGAAAA

mRNA sequence

ATGGATCTTGATCGGGCTGCTCTTTTTCTCTTCAATAATGAGGATATTTCAACTACGGAGGCATTGAGGAAGTTATCTTTGATGCGTCAGTTTGTTATTTCCACTGGTGGAGGTGCCGTTACGAGAACGATAAACTGGAAATATATGCATAAAGGGATCAGTGTCTGGTTGGATGTGCCTTTGGAAGCCTTGGTTAAGAGAATTTCAGCTGTTTCTGATGTTGAAACCCTCGTCCGTTTATCTACTCTCCTGGAAGAGAGGGGTGAAGCATATGCCAACGCTGAAGTCAAAGTTTCCTGCGAAAAAATTGCAGCCAAACTGGGTACTAAAGACGTATCTAATGTCACACCTATGGCCATAGCAATCGAGGCACTCGAACAAATCGAGATCTTTTTGAAGAGAGAAGATGGTTATTGTGCATTTTGATGGCCACAGGAAATGACTCATTTAGGTACATATGAATAAAGGCAATGGGTGGAACAAATTTGATTTTTTCATTATTTCTGGATAATCTTCTCAATCAAGTATCAAGGCATGTTTCAGTTTCACTCAAGGCTGTCCCATATTTATTGATCTCAGTAAGTGTGTTTGATGTAGTGTAGTGTATCTACAATGTATCATCTGAAAAATGTGGATGGTTATAAGTTTCCAAGCATATGGAAAAACTAGCCATTAGTTGTATTTGAAAGAGTTGGTGAATTCATCTAAGTTAAGAACTTGATGATATCATTCAATTAAGGTTATATTATTGAAATACCCCTAAATTAGGTTCTATTGCATTAGATTTGAAAA

Coding sequence (CDS)

ATGGATCTTGATCGGGCTGCTCTTTTTCTCTTCAATAATGAGGATATTTCAACTACGGAGGCATTGAGGAAGTTATCTTTGATGCGTCAGTTTGTTATTTCCACTGGTGGAGGTGCCGTTACGAGAACGATAAACTGGAAATATATGCATAAAGGGATCAGTGTCTGGTTGGATGTGCCTTTGGAAGCCTTGGTTAAGAGAATTTCAGCTGTTTCTGATGTTGAAACCCTCGTCCGTTTATCTACTCTCCTGGAAGAGAGGGGTGAAGCATATGCCAACGCTGAAGTCAAAGTTTCCTGCGAAAAAATTGCAGCCAAACTGGGTACTAAAGACGTATCTAATGTCACACCTATGGCCATAGCAATCGAGGCACTCGAACAAATCGAGATCTTTTTGAAGAGAGAAGATGGTTATTGTGCATTTTGA

Protein sequence

MDLDRAALFLFNNEDISTTEALRKLSLMRQFVISTGGGAVTRTINWKYMHKGISVWLDVPLEALVKRISAVSDVETLVRLSTLLEERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIEALEQIEIFLKREDGYCAF

Homology

[Close]

BLAST of Lsi10G006640 vs. ExPASy Swiss-Prot
Match: Q8GY88 (Shikimate kinase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=SK2 PE=1 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 8.9e-32
Identity = 76/135 (56.30%), Postives = 91/135 (67.41%), Query Frame = 0


Query: 19  TEALRKLSLM-RQFVISTGGGAVTRTINWKYMHKGISVWLDVPLEALVKRISAVSD---- 78
           TEAL+KLSLM  Q V+STGGGAV R INWKYMHKGIS+WLDVPLEAL  RI+AV      
Sbjct: 159 TEALKKLSLMYHQVVVSTGGGAVIRPINWKYMHKGISIWLDVPLEALAHRIAAVGTGSRP 218

Query: 79  -----------VETLVRLSTLLEERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAI 138
                         L RLST+ + RGEAY  A  +VS E I  KLG + VS++TP  IAI
Sbjct: 219 LLHDDESGDTYTAALNRLSTIWDARGEAYTKASARVSLENITLKLGYRSVSDLTPAEIAI 278

[Close]

BLAST of Lsi10G006640 vs. ExPASy Swiss-Prot
Match: Q9SJ05 (Shikimate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=SK1 PE=1 SV=2)

HSP 1 Score: 137.5 bits (345), Expect = 1.2e-31
Identity = 79/148 (53.38%), Postives = 100/148 (67.57%), Query Frame = 0


Query: 6   AALFLFNNEDI---STTEALRKLSLMRQFVISTGGGAVTRTINWKYMHKGISVWLDVPLE 65
           A +F+ + E+      T+AL+KLS   Q V+STGGGAV R INWKYMHKGIS+WLDVPLE
Sbjct: 147 AEIFVHHGENFFRGKETDALKKLSSRYQVVVSTGGGAVIRPINWKYMHKGISIWLDVPLE 206

Query: 66  ALVKRISAV-SDVETLV-------------RLSTLLEERGEAYANAEVKVSCEKIAAKLG 125
           AL  RI+AV +D   L+             RLS + +ERGEAY NA  +VS E IAAK G
Sbjct: 207 ALAHRIAAVGTDSRPLLHDESGDAYSVAFKRLSAIWDERGEAYTNANARVSLENIAAKRG 266

Query: 126 TKDVSNVTPMAIAIEALEQIEIFLKRED 137
            K+VS++TP  IAIEA EQ+  FL++E+
Sbjct: 267 YKNVSDLTPTEIAIEAFEQVLSFLEKEE 294

[Close]

BLAST of Lsi10G006640 vs. ExPASy Swiss-Prot
Match: Q00497 (Shikimate kinase, chloroplastic OS=Solanum lycopersicum OX=4081 GN=SK PE=1 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 7.1e-29
Identity = 79/145 (54.48%), Postives = 92/145 (63.45%), Query Frame = 0


Query: 5   RAALFLFNNEDISTTEALRKLSLMRQFVISTGGGAVTRTINWKYMHKGISVWLDVPLEAL 64
           R   F  +NE    TE L KLSLM + V+STGGGAV R INW++MHKGISVWLDVPLEAL
Sbjct: 155 RGESFFRDNE----TEVLHKLSLMHRLVVSTGGGAVVRPINWRHMHKGISVWLDVPLEAL 214

Query: 65  VKRISA------------VSDV--ETLVRLSTLLEERGEAYANAEVKVSCEKIAAKLGTK 124
            KRI+               DV   TL RL+TL+E RGE YANA  +VS E IA K   K
Sbjct: 215 AKRITTEGTKSRPLLHEESGDVYDTTLKRLTTLMETRGENYANASARVSLENIALK-REK 274

Query: 125 DVSNVTPMAIAIEALEQIEIFLKRE 136
           DV ++TP  I +E L QIE FLK +
Sbjct: 275 DVCHITPAEITLEVLIQIENFLKTQ 294

[Close]

BLAST of Lsi10G006640 vs. ExPASy Swiss-Prot
Match: Q5NTH3 (Shikimate kinase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=SK2 PE=1 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 3.0e-27
Identity = 73/143 (51.05%), Postives = 94/143 (65.73%), Query Frame = 0


Query: 4   DRAALFLFNNEDISTTEALRKLSLMRQFVISTGGGAVTRTINWKYMHKGISVWLDVPLEA 63
           + +  F  +NE    +  LR LS MR+ V++TGGGAV R +NWKYM KG+SVWLDVPL+A
Sbjct: 144 EHSEAFFRDNE----SSVLRDLSSMRRLVVATGGGAVIRPVNWKYMKKGLSVWLDVPLDA 203

Query: 64  LVKRISAV------------SDVETLV--RLSTLLEERGEAYANAEVKVSCEKIAAKLGT 123
           L +RI+ V            SD  T    +LS L E+RG+AYANA+ +VS E+IAAK G 
Sbjct: 204 LARRIAQVGTASRPLLDQPSSDPYTAAFSKLSMLAEQRGDAYANADARVSLEEIAAKQGH 263

Query: 124 KDVSNVTPMAIAIEALEQIEIFL 133
            DVS +TP  IAIEAL +IE F+
Sbjct: 264 DDVSKLTPTDIAIEALLKIENFV 282

[Close]

BLAST of Lsi10G006640 vs. ExPASy Swiss-Prot
Match: Q5NTH4 (Shikimate kinase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=SK1 PE=1 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 1.9e-26
Identity = 66/128 (51.56%), Postives = 88/128 (68.75%), Query Frame = 0


Query: 19  TEALRKLSLMRQFVISTGGGAVTRTINWKYMHKGISVWLDVPLEALVKRISAV------- 78
           +  LR LS M++ V++TGGGAV R +NWKYM KG+SVWLDVPL+AL +RI+ V       
Sbjct: 157 SSVLRDLSSMKRLVVATGGGAVIRPVNWKYMKKGLSVWLDVPLDALARRIAKVGTASRPL 216

Query: 79  -----SDVETLV--RLSTLLEERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIEA 133
                 D  T+   +LS L E+RG+AYANA+V+VS E+IA+K G  DVS +TP  IAIE+
Sbjct: 217 LDQPSGDPYTMAFSKLSMLAEQRGDAYANADVRVSLEEIASKQGHDDVSKLTPTDIAIES 276

[Close]

BLAST of Lsi10G006640 vs. ExPASy TrEMBL
Match: A0A1S3C781 (Shikimate kinase OS=Cucumis melo OX=3656 GN=LOC103497472 PE=3 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 4.3e-53
Identity = 121/153 (79.08%), Postives = 124/153 (81.05%), Query Frame = 0


Query: 6   AALFLFNNEDI---STTEALRKLSLMRQFVISTGGGAVTRTINWKYMHKGISVWLDVPLE 65
           A +F    ED      TEALRKLSLMRQFVISTGGGAVTR+INWKYMHKGISVWLDVPLE
Sbjct: 146 AEIFKVYGEDFFRERETEALRKLSLMRQFVISTGGGAVTRSINWKYMHKGISVWLDVPLE 205

Query: 66  ALVKRISAVSD--------------VETLVRLSTLLEERGEAYANAEVKVSCEKIAAKLG 125
           ALVKRISAV                 +TLVRLSTLLEERGEAYANAEV VSCEKIAAKLG
Sbjct: 206 ALVKRISAVGTNSRPLLHHDSTDAYSKTLVRLSTLLEERGEAYANAEVTVSCEKIAAKLG 265

Query: 126 TKDVSNVTPMAIAIEALEQIEIFLKREDGYCAF 142
           TKDVSNVTPMAIAIEALEQIEIFLKREDGYCAF
Sbjct: 266 TKDVSNVTPMAIAIEALEQIEIFLKREDGYCAF 298

[Close]

BLAST of Lsi10G006640 vs. ExPASy TrEMBL
Match: A0A5A7TS61 (Shikimate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold299G002170 PE=3 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 4.3e-53
Identity = 121/153 (79.08%), Postives = 124/153 (81.05%), Query Frame = 0


Query: 6   AALFLFNNEDI---STTEALRKLSLMRQFVISTGGGAVTRTINWKYMHKGISVWLDVPLE 65
           A +F    ED      TEALRKLSLMRQFVISTGGGAVTR+INWKYMHKGISVWLDVPLE
Sbjct: 146 AEIFKVYGEDFFRERETEALRKLSLMRQFVISTGGGAVTRSINWKYMHKGISVWLDVPLE 205

Query: 66  ALVKRISAVSD--------------VETLVRLSTLLEERGEAYANAEVKVSCEKIAAKLG 125
           ALVKRISAV                 +TLVRLSTLLEERGEAYANAEV VSCEKIAAKLG
Sbjct: 206 ALVKRISAVGTNSRPLLHHDSTDAYSKTLVRLSTLLEERGEAYANAEVTVSCEKIAAKLG 265

Query: 126 TKDVSNVTPMAIAIEALEQIEIFLKREDGYCAF 142
           TKDVSNVTPMAIAIEALEQIEIFLKREDGYCAF
Sbjct: 266 TKDVSNVTPMAIAIEALEQIEIFLKREDGYCAF 298

[Close]

BLAST of Lsi10G006640 vs. ExPASy TrEMBL
Match: A0A0A0LJX8 (Shikimate kinase OS=Cucumis sativus OX=3659 GN=Csa_2G296130 PE=3 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 4.3e-53
Identity = 121/158 (76.58%), Postives = 126/158 (79.75%), Query Frame = 0


Query: 1   MDLDRAALFLFNNEDI---STTEALRKLSLMRQFVISTGGGAVTRTINWKYMHKGISVWL 60
           M +  A +F    ED      TEALRKLSLMRQFVISTGGGAVTR+INWKYMHKGISVWL
Sbjct: 145 MGISVAEVFKVYGEDFFRERETEALRKLSLMRQFVISTGGGAVTRSINWKYMHKGISVWL 204

Query: 61  DVPLEALVKRISAVSD--------------VETLVRLSTLLEERGEAYANAEVKVSCEKI 120
           DVPLEALVKRISAV                 +TLVRLSTLLEERGEAYANAEVKVSCEKI
Sbjct: 205 DVPLEALVKRISAVGTNSRPLLHHDSNDAYSKTLVRLSTLLEERGEAYANAEVKVSCEKI 264

Query: 121 AAKLGTKDVSNVTPMAIAIEALEQIEIFLKREDGYCAF 142
           AAKLGTKDVSNVTPMAIAIEALE+IE FLKREDGYCAF
Sbjct: 265 AAKLGTKDVSNVTPMAIAIEALEEIETFLKREDGYCAF 302

[Close]

BLAST of Lsi10G006640 vs. ExPASy TrEMBL
Match: Q6PLR3 (Shikimate kinase (Fragment) OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 9.5e-53
Identity = 116/137 (84.67%), Postives = 119/137 (86.86%), Query Frame = 0


Query: 19  TEALRKLSLMRQFVISTGGGAVTRTINWKYMHKGISVWLDVPLEALVKRISAVSD----- 78
           TEALRKLSLMRQFVISTGGGAVTR+INWKYMHKGISVWLDVPLEALVKRISAV       
Sbjct: 21  TEALRKLSLMRQFVISTGGGAVTRSINWKYMHKGISVWLDVPLEALVKRISAVGTNSRPL 80

Query: 79  ---------VETLVRLSTLLEERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIEA 138
                     +TLVRLSTLLEERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIEA
Sbjct: 81  LHHDSNDAYSKTLVRLSTLLEERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIEA 140

Query: 139 LEQIEIFLKREDGYCAF 142
           LE+IE FLKREDGYCAF
Sbjct: 141 LEEIETFLKREDGYCAF 157

[Close]

BLAST of Lsi10G006640 vs. ExPASy TrEMBL
Match: E7BBT4 (Shikimate kinase OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 2.8e-52
Identity = 120/158 (75.95%), Postives = 125/158 (79.11%), Query Frame = 0


Query: 1   MDLDRAALFLFNNEDI---STTEALRKLSLMRQFVISTGGGAVTRTINWKYMHKGISVWL 60
           M +  A +F    ED      TEAL KLSLMRQFVISTGGGAVTR+INWKYMHKGISVWL
Sbjct: 145 MGISVAEVFKVYGEDFFRERETEALGKLSLMRQFVISTGGGAVTRSINWKYMHKGISVWL 204

Query: 61  DVPLEALVKRISAVSD--------------VETLVRLSTLLEERGEAYANAEVKVSCEKI 120
           DVPLEALVKRISAV                 +TLVRLSTLLEERGEAYANAEVKVSCEKI
Sbjct: 205 DVPLEALVKRISAVGTNSRPLLHHDSNDAYSKTLVRLSTLLEERGEAYANAEVKVSCEKI 264

Query: 121 AAKLGTKDVSNVTPMAIAIEALEQIEIFLKREDGYCAF 142
           AAKLGTKDVSNVTPMAIAIEALE+IE FLKREDGYCAF
Sbjct: 265 AAKLGTKDVSNVTPMAIAIEALEEIETFLKREDGYCAF 302

[Close]

BLAST of Lsi10G006640 vs. NCBI nr
Match: XP_011649381.1 (shikimate kinase, chloroplastic isoform X1 [Cucumis sativus] >XP_011649382.1 shikimate kinase, chloroplastic isoform X1 [Cucumis sativus] >KGN62088.1 hypothetical protein Csa_006505 [Cucumis sativus])

HSP 1 Score: 217.2 bits (552), Expect = 8.8e-53
Identity = 121/158 (76.58%), Postives = 126/158 (79.75%), Query Frame = 0


Query: 1   MDLDRAALFLFNNEDI---STTEALRKLSLMRQFVISTGGGAVTRTINWKYMHKGISVWL 60
           M +  A +F    ED      TEALRKLSLMRQFVISTGGGAVTR+INWKYMHKGISVWL
Sbjct: 145 MGISVAEVFKVYGEDFFRERETEALRKLSLMRQFVISTGGGAVTRSINWKYMHKGISVWL 204

Query: 61  DVPLEALVKRISAVSD--------------VETLVRLSTLLEERGEAYANAEVKVSCEKI 120
           DVPLEALVKRISAV                 +TLVRLSTLLEERGEAYANAEVKVSCEKI
Sbjct: 205 DVPLEALVKRISAVGTNSRPLLHHDSNDAYSKTLVRLSTLLEERGEAYANAEVKVSCEKI 264

Query: 121 AAKLGTKDVSNVTPMAIAIEALEQIEIFLKREDGYCAF 142
           AAKLGTKDVSNVTPMAIAIEALE+IE FLKREDGYCAF
Sbjct: 265 AAKLGTKDVSNVTPMAIAIEALEEIETFLKREDGYCAF 302

[Close]

BLAST of Lsi10G006640 vs. NCBI nr
Match: XP_008457898.1 (PREDICTED: shikimate kinase, chloroplastic-like [Cucumis melo] >XP_008457899.1 PREDICTED: shikimate kinase, chloroplastic-like [Cucumis melo] >KAA0045894.1 shikimate kinase [Cucumis melo var. makuwa] >TYK13694.1 shikimate kinase [Cucumis melo var. makuwa])

HSP 1 Score: 217.2 bits (552), Expect = 8.8e-53
Identity = 121/153 (79.08%), Postives = 124/153 (81.05%), Query Frame = 0


Query: 6   AALFLFNNEDI---STTEALRKLSLMRQFVISTGGGAVTRTINWKYMHKGISVWLDVPLE 65
           A +F    ED      TEALRKLSLMRQFVISTGGGAVTR+INWKYMHKGISVWLDVPLE
Sbjct: 146 AEIFKVYGEDFFRERETEALRKLSLMRQFVISTGGGAVTRSINWKYMHKGISVWLDVPLE 205

Query: 66  ALVKRISAVSD--------------VETLVRLSTLLEERGEAYANAEVKVSCEKIAAKLG 125
           ALVKRISAV                 +TLVRLSTLLEERGEAYANAEV VSCEKIAAKLG
Sbjct: 206 ALVKRISAVGTNSRPLLHHDSTDAYSKTLVRLSTLLEERGEAYANAEVTVSCEKIAAKLG 265

Query: 126 TKDVSNVTPMAIAIEALEQIEIFLKREDGYCAF 142
           TKDVSNVTPMAIAIEALEQIEIFLKREDGYCAF
Sbjct: 266 TKDVSNVTPMAIAIEALEQIEIFLKREDGYCAF 298

[Close]

BLAST of Lsi10G006640 vs. NCBI nr
Match: AAT02351.1 (shikimate kinase, partial [Cucumis sativus])

HSP 1 Score: 216.1 bits (549), Expect = 2.0e-52
Identity = 116/137 (84.67%), Postives = 119/137 (86.86%), Query Frame = 0


Query: 19  TEALRKLSLMRQFVISTGGGAVTRTINWKYMHKGISVWLDVPLEALVKRISAVSD----- 78
           TEALRKLSLMRQFVISTGGGAVTR+INWKYMHKGISVWLDVPLEALVKRISAV       
Sbjct: 21  TEALRKLSLMRQFVISTGGGAVTRSINWKYMHKGISVWLDVPLEALVKRISAVGTNSRPL 80

Query: 79  ---------VETLVRLSTLLEERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIEA 138
                     +TLVRLSTLLEERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIEA
Sbjct: 81  LHHDSNDAYSKTLVRLSTLLEERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIEA 140

Query: 139 LEQIEIFLKREDGYCAF 142
           LE+IE FLKREDGYCAF
Sbjct: 141 LEEIETFLKREDGYCAF 157

[Close]

BLAST of Lsi10G006640 vs. NCBI nr
Match: NP_001292691.1 (shikimate kinase, chloroplastic [Cucumis sativus] >CBY85385.1 shikimate kinase [Cucumis sativus])

HSP 1 Score: 214.5 bits (545), Expect = 5.7e-52
Identity = 120/158 (75.95%), Postives = 125/158 (79.11%), Query Frame = 0


Query: 1   MDLDRAALFLFNNEDI---STTEALRKLSLMRQFVISTGGGAVTRTINWKYMHKGISVWL 60
           M +  A +F    ED      TEAL KLSLMRQFVISTGGGAVTR+INWKYMHKGISVWL
Sbjct: 145 MGISVAEVFKVYGEDFFRERETEALGKLSLMRQFVISTGGGAVTRSINWKYMHKGISVWL 204

Query: 61  DVPLEALVKRISAVSD--------------VETLVRLSTLLEERGEAYANAEVKVSCEKI 120
           DVPLEALVKRISAV                 +TLVRLSTLLEERGEAYANAEVKVSCEKI
Sbjct: 205 DVPLEALVKRISAVGTNSRPLLHHDSNDAYSKTLVRLSTLLEERGEAYANAEVKVSCEKI 264

Query: 121 AAKLGTKDVSNVTPMAIAIEALEQIEIFLKREDGYCAF 142
           AAKLGTKDVSNVTPMAIAIEALE+IE FLKREDGYCAF
Sbjct: 265 AAKLGTKDVSNVTPMAIAIEALEEIETFLKREDGYCAF 302

[Close]

BLAST of Lsi10G006640 vs. NCBI nr
Match: XP_038902715.1 (shikimate kinase, chloroplastic-like [Benincasa hispida] >XP_038902716.1 shikimate kinase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 214.5 bits (545), Expect = 5.7e-52
Identity = 119/153 (77.78%), Postives = 122/153 (79.74%), Query Frame = 0


Query: 6   AALFLFNNEDI---STTEALRKLSLMRQFVISTGGGAVTRTINWKYMHKGISVWLDVPLE 65
           A +F    ED      TEALRKLSLM QFVISTGGGAVTRTINWKYMHKGISVWLDVPLE
Sbjct: 146 AEIFKVYGEDFFRERETEALRKLSLMHQFVISTGGGAVTRTINWKYMHKGISVWLDVPLE 205

Query: 66  ALVKRISAVSD--------------VETLVRLSTLLEERGEAYANAEVKVSCEKIAAKLG 125
           ALVKRIS V                 +TLVRLSTLLEERGEAYANA+V VSCEKIAAKLG
Sbjct: 206 ALVKRISVVGTSSRPLLHHDSTDAYTKTLVRLSTLLEERGEAYANAKVTVSCEKIAAKLG 265

Query: 126 TKDVSNVTPMAIAIEALEQIEIFLKREDGYCAF 142
           TKDVSNVTPMAIAIEALEQIEIFLKREDGYCAF
Sbjct: 266 TKDVSNVTPMAIAIEALEQIEIFLKREDGYCAF 298

[Close]

BLAST of Lsi10G006640 vs. TAIR 10
Match: AT4G39540.1 (shikimate kinase 2 )

HSP 1 Score: 137.9 bits (346), Expect = 6.3e-33
Identity = 76/135 (56.30%), Postives = 91/135 (67.41%), Query Frame = 0


Query: 19  TEALRKLSLM-RQFVISTGGGAVTRTINWKYMHKGISVWLDVPLEALVKRISAVSD---- 78
           TEAL+KLSLM  Q V+STGGGAV R INWKYMHKGIS+WLDVPLEAL  RI+AV      
Sbjct: 159 TEALKKLSLMYHQVVVSTGGGAVIRPINWKYMHKGISIWLDVPLEALAHRIAAVGTGSRP 218

Query: 79  -----------VETLVRLSTLLEERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAI 138
                         L RLST+ + RGEAY  A  +VS E I  KLG + VS++TP  IAI
Sbjct: 219 LLHDDESGDTYTAALNRLSTIWDARGEAYTKASARVSLENITLKLGYRSVSDLTPAEIAI 278

[Close]

BLAST of Lsi10G006640 vs. TAIR 10
Match: AT4G39540.2 (shikimate kinase 2 )

HSP 1 Score: 137.9 bits (346), Expect = 6.3e-33
Identity = 76/135 (56.30%), Postives = 91/135 (67.41%), Query Frame = 0


Query: 19  TEALRKLSLM-RQFVISTGGGAVTRTINWKYMHKGISVWLDVPLEALVKRISAVSD---- 78
           TEAL+KLSLM  Q V+STGGGAV R INWKYMHKGIS+WLDVPLEAL  RI+AV      
Sbjct: 159 TEALKKLSLMYHQVVVSTGGGAVIRPINWKYMHKGISIWLDVPLEALAHRIAAVGTGSRP 218

Query: 79  -----------VETLVRLSTLLEERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAI 138
                         L RLST+ + RGEAY  A  +VS E I  KLG + VS++TP  IAI
Sbjct: 219 LLHDDESGDTYTAALNRLSTIWDARGEAYTKASARVSLENITLKLGYRSVSDLTPAEIAI 278

[Close]

BLAST of Lsi10G006640 vs. TAIR 10
Match: AT4G39540.3 (shikimate kinase 2 )

HSP 1 Score: 137.9 bits (346), Expect = 6.3e-33
Identity = 76/135 (56.30%), Postives = 91/135 (67.41%), Query Frame = 0


Query: 19  TEALRKLSLM-RQFVISTGGGAVTRTINWKYMHKGISVWLDVPLEALVKRISAVSD---- 78
           TEAL+KLSLM  Q V+STGGGAV R INWKYMHKGIS+WLDVPLEAL  RI+AV      
Sbjct: 167 TEALKKLSLMYHQVVVSTGGGAVIRPINWKYMHKGISIWLDVPLEALAHRIAAVGTGSRP 226

Query: 79  -----------VETLVRLSTLLEERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAI 138
                         L RLST+ + RGEAY  A  +VS E I  KLG + VS++TP  IAI
Sbjct: 227 LLHDDESGDTYTAALNRLSTIWDARGEAYTKASARVSLENITLKLGYRSVSDLTPAEIAI 286

[Close]

BLAST of Lsi10G006640 vs. TAIR 10
Match: AT2G21940.1 (shikimate kinase 1 )

HSP 1 Score: 137.5 bits (345), Expect = 8.3e-33
Identity = 79/148 (53.38%), Postives = 100/148 (67.57%), Query Frame = 0


Query: 6   AALFLFNNEDI---STTEALRKLSLMRQFVISTGGGAVTRTINWKYMHKGISVWLDVPLE 65
           A +F+ + E+      T+AL+KLS   Q V+STGGGAV R INWKYMHKGIS+WLDVPLE
Sbjct: 147 AEIFVHHGENFFRGKETDALKKLSSRYQVVVSTGGGAVIRPINWKYMHKGISIWLDVPLE 206

Query: 66  ALVKRISAV-SDVETLV-------------RLSTLLEERGEAYANAEVKVSCEKIAAKLG 125
           AL  RI+AV +D   L+             RLS + +ERGEAY NA  +VS E IAAK G
Sbjct: 207 ALAHRIAAVGTDSRPLLHDESGDAYSVAFKRLSAIWDERGEAYTNANARVSLENIAAKRG 266

Query: 126 TKDVSNVTPMAIAIEALEQIEIFLKRED 137
            K+VS++TP  IAIEA EQ+  FL++E+
Sbjct: 267 YKNVSDLTPTEIAIEAFEQVLSFLEKEE 294

[Close]

BLAST of Lsi10G006640 vs. TAIR 10
Match: AT2G21940.2 (shikimate kinase 1 )

HSP 1 Score: 137.5 bits (345), Expect = 8.3e-33
Identity = 79/148 (53.38%), Postives = 100/148 (67.57%), Query Frame = 0


Query: 6   AALFLFNNEDI---STTEALRKLSLMRQFVISTGGGAVTRTINWKYMHKGISVWLDVPLE 65
           A +F+ + E+      T+AL+KLS   Q V+STGGGAV R INWKYMHKGIS+WLDVPLE
Sbjct: 147 AEIFVHHGENFFRGKETDALKKLSSRYQVVVSTGGGAVIRPINWKYMHKGISIWLDVPLE 206

Query: 66  ALVKRISAV-SDVETLV-------------RLSTLLEERGEAYANAEVKVSCEKIAAKLG 125
           AL  RI+AV +D   L+             RLS + +ERGEAY NA  +VS E IAAK G
Sbjct: 207 ALAHRIAAVGTDSRPLLHDESGDAYSVAFKRLSAIWDERGEAYTNANARVSLENIAAKRG 266

Query: 126 TKDVSNVTPMAIAIEALEQIEIFLKRED 137
            K+VS++TP  IAIEA EQ+  FL++E+
Sbjct: 267 YKNVSDLTPTEIAIEAFEQVLSFLEKEE 294

The following BLAST results are available for this feature:

BLAST of Lsi10G006640 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GY88	8.9e-32	56.30	Shikimate kinase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=SK2 PE=1 SV...	[more]
Q9SJ05	1.2e-31	53.38	Shikimate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=SK1 PE=1 SV...	[more]
Q00497	7.1e-29	54.48	Shikimate kinase, chloroplastic OS=Solanum lycopersicum OX=4081 GN=SK PE=1 SV=1	[more]
Q5NTH3	3.0e-27	51.05	Shikimate kinase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=SK...	[more]
Q5NTH4	1.9e-26	51.56	Shikimate kinase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=SK...	[more]

BLAST of Lsi10G006640 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3C781	4.3e-53	79.08	Shikimate kinase OS=Cucumis melo OX=3656 GN=LOC103497472 PE=3 SV=1	[more]
A0A5A7TS61	4.3e-53	79.08	Shikimate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold299G002...	[more]
A0A0A0LJX8	4.3e-53	76.58	Shikimate kinase OS=Cucumis sativus OX=3659 GN=Csa_2G296130 PE=3 SV=1	[more]
Q6PLR3	9.5e-53	84.67	Shikimate kinase (Fragment) OS=Cucumis sativus OX=3659 PE=2 SV=1	[more]
E7BBT4	2.8e-52	75.95	Shikimate kinase OS=Cucumis sativus OX=3659 PE=2 SV=1	[more]

BLAST of Lsi10G006640 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011649381.1	8.8e-53	76.58	shikimate kinase, chloroplastic isoform X1 [Cucumis sativus] >XP_011649382.1 shi...	[more]
XP_008457898.1	8.8e-53	79.08	PREDICTED: shikimate kinase, chloroplastic-like [Cucumis melo] >XP_008457899.1 P...	[more]
AAT02351.1	2.0e-52	84.67	shikimate kinase, partial [Cucumis sativus]	[more]
NP_001292691.1	5.7e-52	75.95	shikimate kinase, chloroplastic [Cucumis sativus] >CBY85385.1 shikimate kinase [...	[more]
XP_038902715.1	5.7e-52	77.78	shikimate kinase, chloroplastic-like [Benincasa hispida] >XP_038902716.1 shikima...	[more]

BLAST of Lsi10G006640 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G39540.1	6.3e-33	56.30	shikimate kinase 2	[more]
AT4G39540.2	6.3e-33	56.30	shikimate kinase 2	[more]
AT4G39540.3	6.3e-33	56.30	shikimate kinase 2	[more]
AT2G21940.1	8.3e-33	53.38	shikimate kinase 1	[more]
AT2G21940.2	8.3e-33	53.38	shikimate kinase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR01100	SHIKIMTKNASE	coord: 32..41 score: 66.15 coord: 52..69 score: 34.62
None	No IPR available	PANTHER	PTHR21087:SF22	SHIKIMATE KINASE 2, CHLOROPLASTIC	coord: 18..138
None	No IPR available	PANTHER	PTHR21087	SHIKIMATE KINASE	coord: 18..138
IPR031322	Shikimate kinase/gluconokinase	PFAM	PF01202	SKI	coord: 19..109 e-value: 7.0E-16 score: 58.8
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 16..139 e-value: 2.0E-28 score: 101.7

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi10G006640.1	Lsi10G006640.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008652	cellular amino acid biosynthetic process
biological_process	GO:0009423	chorismate biosynthetic process
biological_process	GO:0016310	phosphorylation
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004765	shikimate kinase activity