Lsi10G006560 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi10G006560
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Pectinesterase
Location	chr10: 9123500 .. 9129145 (-)
RNA-Seq Expression	Lsi10G006560
Synteny	Lsi10G006560

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATGTTTTAAAAAAATTGCATTCATCCTTTTTGTTTCCATAAAATGAAATTTTAAACCCTTTTTATTATATATATATATCTCCTTTTTTTTTTTTTATTTATAGTCACACTTTATTCCGGATTGACCTTCTAAAATTAAGATGAGCATTAATATCAAAATACTAGTACTATGCTATGGTAAGCTAGGACAAGGTGTGACATTTATTATCATTAAAAAAAAAAAAGGTTGTGTTCACTCATACTCAAATCATAACAACTCTTCATAAAATGTTATAGGGTTGTCACACATGGTGCTTTAACCTCTCACCATACTTAAACTAGAATACATTTTTATGGCACATTTATACTTAAAAGAAACATAACAAAGAAATTAAAACAAAGCAATAAATTTGGGAACACAATAATTAATGTGAAAAAACTTTAAAGTGAAGAAAAAACTACAGACCATAAAGAAATTTCACTATGTGAAAAATTATCACAATCACACAAAAATCTCTCCTCTACCCCAATTACAAAAGAAAACATTCTCTCAAAGCATTATATCACTCACTCAACTGAGTTAGTATACCACGCTTAAAGTTCTTCCAAATAGAGATGATTTGAAGCCAAAAGCATAGGCTCTTTTTATAATGTTTTGAGTTCATCACTTTCTTGAACTTTTTCGATATGGGACAACTACTCTTCTAATATTTTGCCAAAATCCAATAAGTTAAGACCTACAATCTAAAATAGAGAACATGTCAGATTATTACGAAGATATGTGAAGGTGGTTGTCCCTAATATTAAGCCATCAACAAAAGGATTGTATATCCAAAGATAGATATGAGATGTTAGCCGGTTGGCAACTACGCATATGATGAAACGTTGACAATCAACCATTCACACTCTGTTGGTGAGAATTAATGTATATAAAACCTCAAATTGCCCTTTGTGTATGCAACTAAACAATATTATATTCAATTCCCAATATAATAACACATACTTGCATGCACGTTTGCTTCACAAATACAATACATCCTTCCTCCTTCCTCCTTTCTCCCTTCCCTCCCTAAATTTAAGGAATTTATCATTTTAAATCTTGAACTTTTGTAAATGTATCAATTTATACCCATTTGTTAAATTCTATTTTAAAAATCTCGTGTAAAACTTATAATTGTATCAATTAAACTCCTAAACTTTCATAAATGAATCAATTTACTTTTAGTAAAAAAAATTGAGAATCGTCTATGCATTTACTCTCATTGTCCATTTTGTAAATCCAACATTTGAAGAACTCTACACATATTCCAAAAAATGCCAAAAGACTAAGGAGTTATGGGTTCAATCCATGGTGGCTACTTACCTAGGGTTTAATATCCTACGGGTTTCCTTGACACCCAAATGTTGTAGGGTCAGGCGGGTTGTCCCGTGAGATTAGTCGAGGTGCGCATAAGCTGATCCGGACACTCACAAATATATATATATATAAAAAAAGAACTCTACACATATTTGAGAATTAATGCATGGATAATTTTTACATATAATTGTAATGAATAGTCTAAAGATTGATTTATGAATATTTTAGGAGTTTAATTGACTCAAATTATAAATTTTGTGATATTGATGGAACGATATTTGGAGGAAGGTATGAATTCATAGTTAATTCCTATCTAAGTATTGTTACAAAGCAAAATTAAACAACTTGTTGATTAAAAGGAACATGAATTATATTATTCTTCATATTTCACGTGGGGAAGGCAAATTTATCCTTATAAATAAGCGATATATGTAAAGCCAAGCCAAAAAAAGAAACAAACACATACACACCATGGCTTCTTCAAATCCCATATCTCTCCTCACATTTCTCACTTTCCTTTTCCTCCTCTCTTCTTCATCGTTCGCCGTCGTTCCAACTGCGGAGATTTGCCAATCCACCACGTCCCCCTCCTTCTGCAAATCTTTCCTTCCCAACTCCCCCGCCTCCATCCACTCACATTGTCGCTTCACTCTCCGCAACGCCCTCAACCATGCACGCACCTTCTTGACACTCATCGACGCCCACCTCAACCTCCTTCCCTTCCCTTCCGCCCTCCACGACTGTCGTGGCCTTGCGGAAGCCAACCTCGACTTCATATCCCAAACTTTCTCCATAGTCAACTTCACAACCATGGCCCTTCCTTATTACCAAGCCCATGAGATGATGTCCTTGATAAGTGCAATGATCACCAATGAAGACACTTGTTATGAAGGACTGGTAGGTTTGGGCTCGGTCGTGGGCTTGGTTGATAAGGTGTTGGAGGCCATCTCTTTTGATAGAAAGCTATATAGTTTGTATCTTTCTTTGTTCAAAATGGGTTGGGTTTCTGAGGACATGAAGGCTCCTATGTTGCCAAACATGAACCATTTTGGGGTGGGGAAAGGGCAGTTGAAGCTTAAAATGTCGCCCAAAGACCGTGCGTATTATGAGCGCTTGGTTCATAGGAAGAAGCCCGCGGCCGCTAGAAGGCTTCTTCAAACCAATTACGAAGATGATGGAATTTTGATCAATGGTAATAACCTTTCGTTATTCTTTTGCAAATTTTTTTACAAAATTACAAAAATACCCTTTTTTCAAAAATTCTATATACTTTCAAATTTTACTATATTAATTAAAACTACGGAAGATTTTTGAAGATTTTGAGTTTAGTTTCTATTTGAGATATGTTTCAATTTACTGTCTAAGTTTCAAAATAAACTAAGTTTTGTTATAATTTAGTTCCTAGGTTTCAAGATTTATAGTTTTAACCTTGATTTTTCATTCAATACTCATTTTCAATTATTAACGTTTATTCTATTAATTAATTTAAAATAATTAGAAAGTGAAATTTAAATTTTAAATAGTGAAACTTAATTAATTAAATTTTTTTATTTTTTAAAAATTAATTAATTGAAATTAACCCTAAAGATGAAAAGTGAAGTATGTAATGAAAAGTCGAGGTTGAAAGTGTACATCTTGAAATCTATTGATCAAATTGAAACAAAATTCAAATCTTAATGGTAAAAATATTGTAACATATTGAAATCTAGAGACTAAATTGAAACCAAACTCAATACTTAATGATTAAATATGTAACATTTAAAACTTAGAGGCCAAATTGAAATTATATTCAAAATTTATGGATCAAAAAAGTATTACCCCTCTCCCCCCTTAAAACTACTTTTGGAACATGAAATTCGTTAGGATGTTTGATAGAAATTAGGATGATTGAAAAAGATGTAAAGATGACGTAGCAAAAATGAGAGTCAAGACACTATCACATCATTTTAGGTCACCACGACATTGTTTCAAATCTCAATTTACATCTAATATTCTAAGAAGAAAAACTTGGGTGCAATCCAACAAGCTTCCAATGGAATTGTGCCACGTGGCACAAAAAAATTGTGAGTAGAAGAAAGGGAGAGTATGGGAGCTGCACCTAATCATTCTCCATATTCTAATGGGATAATTGTCACTCCAATTGTAGCTTTGATGCATTTATAAAAAAAGTAATAAAAAAAAAATATTGCTACTTTTAAAACAATATCGACATTTTCTATAACAAATAGCAAATTAAAGATTGGATTTTTCTTTTCATAATTTAGGCCTTCATGATGGATATTAATTCTTTTGTTCAATAGGCATGGTTGCGGTGGATCAAAATGGAACATACGACTTCACAACTATCAGCGACGCCATTGCGGCAGCTCCGAACAAGTCTGTCATCACTAAAGGATACTTCTTGATCTATGTTACTGCCGGAATTTACAACGAAAGTGTGTTGGTTCCAAAAGAAAAAAGGTATGTGTTGATGATCGGAGATGGTAACAACGAAACCATCATCACCGGCAACAAAAACGTCGTTGATGGCTCAACCACTTTCAATAGTGCAACTGTTGGTGAGCTCCTTTAAAATCTAGTCACAACTATAAAATTTCTCTAAATTTCTCTAACATGTCCAAGATTTTAGATGATGACATTTTATTATATTTATTAATATTTTGACTCATTTTGTTATATTGAAAACAACTCTTAATTAAACGTCAATCTCATGATACAAGTTTCGAAATTTGAGTAAATGTCAATGAAAATATTTTAAAGTTTAATTTAAAGAATATTTTTGCAATAATAAAAATTAAAGACGGAATACACACTCTTAAGTCATTAGGTTTAAAGTAGATATTAATTTTGTTTATAAGATTTCAAATGCTACATTTTTAATCTAGAGATTTAAAAAATATATCTAAATAGTTTTTGAGCTTAAATTGATGAGTATTTAATTAATTGTTGATTGTACTTGTTTATATATCAAATTGCATTAAATTTTAATGATTGGATAACAAAAGTTACATCTCCTTGCTTTTTCTCTCCCTTCTTTTTTCCTCTTAATTAATTTACAAGTTTTAGTTTAGATAGTTTTTTGAAGTCGTATCTATAGGCCTCTCAAACTTTAAAAATACTCGTTAGTATGTAAACTCGTTAAAAATTGAGAAATTTTCATAGATAAAAAAAGAAGTCAAATTATTTATAGAAAATAACAAAAAAAAAATACTGGTAGATATTGATAGACTTCTATCAATATGTATCAATAATAAACTTCTATCATTTCTATCACTTATAGATCCTCATAGACATCTATCACAACTATCTAAAATTTTTGTTATTTTATGTAAATAATTTTTTTTATTTTTCTATTTTGAAAAATTCCTCCAAAAAAATTTAAAGATTTATTAAATATAAAGTTTACGTACTTAAACTTATATTTATACTAAATTATTATTGCTCGCAGCTGTGGAGGGAACAGGGTTCTTCGGAGTGAACCTAACGATCAGCAACACAGCCGGCGCCGCCAAACACCAAGCGGTAGCGCTGCGAGTGAGCGCTGACAATGTGACGTTATACAGTTGCATTTTCGAAGGCTATCAAGACACTCTCTACACACACTCCCTCCGTCAATTCTACAGAGAATGTGACATCTACGGTACCGTCGACTTCATCTTCGGCAATGCTGCCGTCGTCTTGCAAAACTGCAACATTTACGCCCGCCTCCCGTTGAGTGGCCAGTTTAATGCCCTCACGGCGCAGGGTCGGACCGACCCAAATCAGAACACTGGAACCTCTATCCACAACTGCACCATCAAGCCGACGCCGGAGCTTGCCGCGAGCCCCGCCACAAAGAGCTACTTGGGGCGGCCGTGGAAGGAATATTCTCGAACAGTTTACATGCAGTCGTTTATTGGTGCCTTTATTGATCCTGCAGGCTGGAAGGAGTGGGACGGCACTTTGAATTTAAATACTTCTTATTACGCCGAGTTTAATAACTCAGGGCCGGGCTCCGACACTTCACGGCGGGCTTCGTGGGTGGTCGGTGTCATCAACGCCACCGTTGCTAATAACTTCACTGTGTCTCAATTCTTGGCCGGAGATCAATGGCTGCCACCCACCGCCGTGCCCTACACCGGGGGTTTGATATCATAAATTACCAAATTAATTTCTTGATTTTCTGTTGGATTTTAATTTGATGTATTGATTTCTTTGAAAGGTGGTGGATGATTTATGTTTTGTTTCTTTGTATTATTATAAATTTAATGGAGGTTTTTGTTATTAATTTTTTGCCACTAGTTTATTTATTTATTTATTTTTCAAAGG

mRNA sequence

ATGATCCAAGCCAAAAAAAGAAACAAACACATACACACCATGGCTTCTTCAAATCCCATATCTCTCCTCACATTTCTCACTTTCCTTTTCCTCCTCTCTTCTTCATCGTTCGCCGTCGTTCCAACTGCGGAGATTTGCCAATCCACCACGTCCCCCTCCTTCTGCAAATCTTTCCTTCCCAACTCCCCCGCCTCCATCCACTCACATTGTCGCTTCACTCTCCGCAACGCCCTCAACCATGCACGCACCTTCTTGACACTCATCGACGCCCACCTCAACCTCCTTCCCTTCCCTTCCGCCCTCCACGACTGTCGTGGCCTTGCGGAAGCCAACCTCGACTTCATATCCCAAACTTTCTCCATAGTCAACTTCACAACCATGGCCCTTCCTTATTACCAAGCCCATGAGATGATGTCCTTGATAAGTGCAATGATCACCAATGAAGACACTTGTTATGAAGGACTGGTAGGTTTGGGCTCGGTCGTGGGCTTGGTTGATAAGGTGTTGGAGGCCATCTCTTTTGATAGAAAGCTATATAGTTTGTATCTTTCTTTGTTCAAAATGGGTTGGGTTTCTGAGGACATGAAGGCTCCTATGTTGCCAAACATGAACCATTTTGGGGTGGGGAAAGGGCAGTTGAAGCTTAAAATGTCGCCCAAAGACCGTGCGTATTATGAGCGCTTGGTTCATAGGAAGAAGCCCGCGGCCGCTAGAAGGCTTCTTCAAACCAATTACGAAGATGATGGAATTTTGATCAATGGTAATAACCTTTCCGACGCCATTGCGGCAGCTCCGAACAAGTCTGTCATCACTAAAGGATACTTCTTGATCTATGTTACTGCCGGAATTTACAACGAAAGTGTGTTGGTTCCAAAAGAAAAAAGGTATGTGTTGATGATCGGAGATGGTAACAACGAAACCATCATCACCGGCAACAAAAACGTCGTTGATGGCTCAACCACTTTCAATAGTGCAACTGTTGCTGTGGAGGGAACAGGGTTCTTCGGAGTGAACCTAACGATCAGCAACACAGCCGGCGCCGCCAAACACCAAGCGGTAGCGCTGCGAGTGAGCGCTGACAATGTGACGTTATACAGTTGCATTTTCGAAGGCTATCAAGACACTCTCTACACACACTCCCTCCGTCAATTCTACAGAGAATGTGACATCTACGGTACCGTCGACTTCATCTTCGGCAATGCTGCCGTCGTCTTGCAAAACTGCAACATTTACGCCCGCCTCCCGTTGAGTGGCCAGTTTAATGCCCTCACGGCGCAGGGTCGGACCGACCCAAATCAGAACACTGGAACCTCTATCCACAACTGCACCATCAAGCCGACGCCGGAGCTTGCCGCGAGCCCCGCCACAAAGAGCTACTTGGGGCGGCCGTGGAAGGAATATTCTCGAACAGTTTACATGCAGTCGTTTATTGGTGCCTTTATTGATCCTGCAGGCTGGAAGGAGTGGGACGGCACTTTGAATTTAAATACTTCTTATTACGCCGAGTTTAATAACTCAGGGCCGGGCTCCGACACTTCACGGCGGGCTTCGTGGGTGGTCGGTGTCATCAACGCCACCGTTGCTAATAACTTCACTGTGTCTCAATTCTTGGCCGGAGATCAATGGCTGCCACCCACCGCCGTGCCCTACACCGGGGGTTTGATATCATAAATTACCAAATTAATTTCTTGATTTTCTGTTGGATTTTAATTTGATGTATTGATTTCTTTGAAAGGTGGTGGATGATTTATGTTTTGTTTCTTTGTATTATTATAAATTTAATGGAGGTTTTTGTTATTAATTTTTTGCCACTAGTTTATTTATTTATTTATTTTTCAAAGG

Coding sequence (CDS)

ATGATCCAAGCCAAAAAAAGAAACAAACACATACACACCATGGCTTCTTCAAATCCCATATCTCTCCTCACATTTCTCACTTTCCTTTTCCTCCTCTCTTCTTCATCGTTCGCCGTCGTTCCAACTGCGGAGATTTGCCAATCCACCACGTCCCCCTCCTTCTGCAAATCTTTCCTTCCCAACTCCCCCGCCTCCATCCACTCACATTGTCGCTTCACTCTCCGCAACGCCCTCAACCATGCACGCACCTTCTTGACACTCATCGACGCCCACCTCAACCTCCTTCCCTTCCCTTCCGCCCTCCACGACTGTCGTGGCCTTGCGGAAGCCAACCTCGACTTCATATCCCAAACTTTCTCCATAGTCAACTTCACAACCATGGCCCTTCCTTATTACCAAGCCCATGAGATGATGTCCTTGATAAGTGCAATGATCACCAATGAAGACACTTGTTATGAAGGACTGGTAGGTTTGGGCTCGGTCGTGGGCTTGGTTGATAAGGTGTTGGAGGCCATCTCTTTTGATAGAAAGCTATATAGTTTGTATCTTTCTTTGTTCAAAATGGGTTGGGTTTCTGAGGACATGAAGGCTCCTATGTTGCCAAACATGAACCATTTTGGGGTGGGGAAAGGGCAGTTGAAGCTTAAAATGTCGCCCAAAGACCGTGCGTATTATGAGCGCTTGGTTCATAGGAAGAAGCCCGCGGCCGCTAGAAGGCTTCTTCAAACCAATTACGAAGATGATGGAATTTTGATCAATGGTAATAACCTTTCCGACGCCATTGCGGCAGCTCCGAACAAGTCTGTCATCACTAAAGGATACTTCTTGATCTATGTTACTGCCGGAATTTACAACGAAAGTGTGTTGGTTCCAAAAGAAAAAAGGTATGTGTTGATGATCGGAGATGGTAACAACGAAACCATCATCACCGGCAACAAAAACGTCGTTGATGGCTCAACCACTTTCAATAGTGCAACTGTTGCTGTGGAGGGAACAGGGTTCTTCGGAGTGAACCTAACGATCAGCAACACAGCCGGCGCCGCCAAACACCAAGCGGTAGCGCTGCGAGTGAGCGCTGACAATGTGACGTTATACAGTTGCATTTTCGAAGGCTATCAAGACACTCTCTACACACACTCCCTCCGTCAATTCTACAGAGAATGTGACATCTACGGTACCGTCGACTTCATCTTCGGCAATGCTGCCGTCGTCTTGCAAAACTGCAACATTTACGCCCGCCTCCCGTTGAGTGGCCAGTTTAATGCCCTCACGGCGCAGGGTCGGACCGACCCAAATCAGAACACTGGAACCTCTATCCACAACTGCACCATCAAGCCGACGCCGGAGCTTGCCGCGAGCCCCGCCACAAAGAGCTACTTGGGGCGGCCGTGGAAGGAATATTCTCGAACAGTTTACATGCAGTCGTTTATTGGTGCCTTTATTGATCCTGCAGGCTGGAAGGAGTGGGACGGCACTTTGAATTTAAATACTTCTTATTACGCCGAGTTTAATAACTCAGGGCCGGGCTCCGACACTTCACGGCGGGCTTCGTGGGTGGTCGGTGTCATCAACGCCACCGTTGCTAATAACTTCACTGTGTCTCAATTCTTGGCCGGAGATCAATGGCTGCCACCCACCGCCGTGCCCTACACCGGGGGTTTGATATCATAA

Protein sequence

MIQAKKRNKHIHTMASSNPISLLTFLTFLFLLSSSSFAVVPTAEICQSTTSPSFCKSFLPNSPASIHSHCRFTLRNALNHARTFLTLIDAHLNLLPFPSALHDCRGLAEANLDFISQTFSIVNFTTMALPYYQAHEMMSLISAMITNEDTCYEGLVGLGSVVGLVDKVLEAISFDRKLYSLYLSLFKMGWVSEDMKAPMLPNMNHFGVGKGQLKLKMSPKDRAYYERLVHRKKPAAARRLLQTNYEDDGILINGNNLSDAIAAAPNKSVITKGYFLIYVTAGIYNESVLVPKEKRYVLMIGDGNNETIITGNKNVVDGSTTFNSATVAVEGTGFFGVNLTISNTAGAAKHQAVALRVSADNVTLYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFNALTAQGRTDPNQNTGTSIHNCTIKPTPELAASPATKSYLGRPWKEYSRTVYMQSFIGAFIDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRASWVVGVINATVANNFTVSQFLAGDQWLPPTAVPYTGGLIS

Homology

[Close]

BLAST of Lsi10G006560 vs. ExPASy Swiss-Prot
Match: O22256 (Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3702 GN=PME20 PE=2 SV=2)

HSP 1 Score: 509.6 bits (1311), Expect = 4.4e-143
Identity = 275/531 (51.79%), Postives = 349/531 (65.73%), Query Frame = 0


Query: 43  AEICQSTTSPSFCKSFLPNSPASIHSHCRFTLRNALNHARTFLTLIDAHLN------LLP 102
           + IC+    PS+C+S LPN P  I+S+ R +LR +L+ AR F+++IDA L+         
Sbjct: 36  SNICRFAPDPSYCRSVLPNQPGDIYSYGRLSLRRSLSRARRFISMIDAELDRKGKVAAKS 95

Query: 103 FPSALHDCRGLAEANLDFISQTFSIVNFTTMALPYYQAHEMMSLISAMITNEDTCYEGLV 162
              AL DC+ LA   +D++  +    + +T  L   +A ++ + +SA ITNE TC EGL 
Sbjct: 96  TVGALEDCKFLASLTMDYLLSSSQTAD-STKTLSLSRAEDVHTFLSAAITNEQTCLEGLK 155

Query: 163 GLGSVVGLVDKVLEAISFDRKLYSLYLSLFKMGWV-----SEDMKAPMLPNMNHFGVGKG 222
              S  GL   +      D KLY + L+LF  GWV     S  +  P       FG   G
Sbjct: 156 STASENGLSGDLFN----DTKLYGVSLALFSKGWVPRRQRSRPIWQPQARFKKFFGFRNG 215

Query: 223 QLKLKMSPKDRAYYERLVHRKKPAAARRLLQTNYEDDGILING----NNLSDAIAAAPNK 282
           +L LKM+ + RA Y  +  RK   +    +Q + +   ++ NG      ++ AIAAAPNK
Sbjct: 216 KLPLKMTERARAVYNTVTRRKLLQSDADAVQVS-DIVTVIQNGTGNFTTINAAIAAAPNK 275

Query: 283 SVITKGYFLIYVTAGIYNESVLVPKEKRYVLMIGDGNNETIITGNKNVVDGSTTFNSATV 342
           +  + GYFLIYVTAG+Y E V VPK KRYV+MIGDG N+T+ITGN++VVDG TTFNSAT 
Sbjct: 276 TDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGINQTVITGNRSVVDGWTTFNSATF 335

Query: 343 AVEGTGFFGVNLTISNTAGAAKHQAVALRVSADNVTLYSCIFEGYQDTLYTHSLRQFYRE 402
            + G  F GVN+TI NTAG  K QAVALR   D    YSC FE YQDTLYTHSLRQFYRE
Sbjct: 336 ILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSVFYSCSFEAYQDTLYTHSLRQFYRE 395

Query: 403 CDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFNALTAQGRTDPNQNTGTSIHNCTIKPT 462
           CD+YGTVDFIFGNAAVVLQNCN+Y R P  GQ N +TAQGRTDPNQNTGT+IH CTI+P 
Sbjct: 396 CDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEVTAQGRTDPNQNTGTAIHGCTIRPA 455

Query: 463 PELAASPAT-KSYLGRPWKEYSRTVYMQSFIGAFIDPAGWKEWDGTLNLNTSYYAEFNNS 522
            +LA S  T K+YLGRPWKEYSRTV MQ++I  F++P+GW  W G   L+T YYAE+NN+
Sbjct: 456 DDLATSNYTVKTYLGRPWKEYSRTVVMQTYIDGFLEPSGWNAWSGDFALSTLYYAEYNNT 515

Query: 523 GPGSDTSRRASWV-VGVINATVANNFTVSQFLAGDQWLPPTAVPYTGGLIS 557
           GPGSDT+ R +W    VINAT A+NFTV+ FL G+ W+  T VP+ GGLI+
Sbjct: 516 GPGSDTTNRVTWPGYHVINATDASNFTVTNFLVGEGWIGQTGVPFVGGLIA 560

[Close]

BLAST of Lsi10G006560 vs. ExPASy Swiss-Prot
Match: Q8RXK7 (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3702 GN=PME41 PE=2 SV=2)

HSP 1 Score: 493.8 bits (1270), Expect = 2.5e-138
Identity = 280/566 (49.47%), Postives = 358/566 (63.25%), Query Frame = 0


Query: 20  ISLLTFLTFLFLLSSSSFAVVPTAEICQSTTSPSFCKSFLP-NSPASIHSHCRFTLRNAL 79
           ++L   L  LF+ S +      ++ IC++T  P FCKS  P  S   +  + RF+LR +L
Sbjct: 9   VTLFLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFPQTSQGDVREYGRFSLRKSL 68

Query: 80  NHARTFLTLIDAHL---NLLPFPS---ALHDCRGLAEANLDFISQTFSIVNFTTMA--LP 139
             +R F   ID +L   N L   S   AL DCR LA    D++  +F  VN TT +  L 
Sbjct: 69  TQSRKFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETVNITTSSKTLS 128

Query: 140 YYQAHEMMSLISAMITNEDTCYEGL-VGLGSVVGLVDKVLEAISFDRKLYSLYLSLFKMG 199
           + +A E+ +L+SA +TNE TC +G+     S   + + V   +  D KL+S+ L+LF  G
Sbjct: 129 FSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSVSLALFTKG 188

Query: 200 WVSEDMKAPML-----PNMNHFGV------GKGQLKLKMSPKDRAYYERLVHRKKPAAAR 259
           WV +  K         P   H           G L LKM+   RA YE L  R+K A   
Sbjct: 189 WVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESL-SRRKLADDD 248

Query: 260 RLLQTNYEDDGILINGN------NLSDAIAAAPNKSVITKGYFLIYVTAGIYNESVLVPK 319
             + T    D + +N N       +++A+ +APNK+  T GYF+IYVT+G+Y E+V++ K
Sbjct: 249 NDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVVIAK 308

Query: 320 EKRYVLMIGDGNNETIITGNKNVVDGSTTFNSATVAVEGTGFFGVNLTISNTAGAAKHQA 379
            KRY++MIGDG N T++TGN+NVVDG TTFNSAT AV    F  VN+T  NTAG  KHQA
Sbjct: 309 NKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPEKHQA 368

Query: 380 VALRVSADNVTLYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYA 439
           VA+R SAD    YSC FE YQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVV Q+CN+Y 
Sbjct: 369 VAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNLYP 428

Query: 440 RLPLSGQFNALTAQGRTDPNQNTGTSIHNCTIKPTPELAASPAT-KSYLGRPWKEYSRTV 499
           R P+  QFNA+TAQGRTDPNQNTG SIHNCTIKP  +L +S  T K+YLGRPWKEYSRTV
Sbjct: 429 RQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTV 488

Query: 500 YMQSFIGAFIDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRASWV-VGVINATVANN 557
           +MQS+I   ++P GW+EW+G   L+T YYAE+NN+G GS T+ R  W    VIN+T ANN
Sbjct: 489 FMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVINSTDANN 548

[Close]

BLAST of Lsi10G006560 vs. ExPASy Swiss-Prot
Match: Q9SRX4 (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PME7 PE=2 SV=1)

HSP 1 Score: 485.3 bits (1248), Expect = 9.0e-136
Identity = 275/576 (47.74%), Postives = 356/576 (61.81%), Query Frame = 0


Query: 22  LLTFLTFLFLLSSSSFAVVPTAEICQSTTSPSFCKSFLPNSPASIHSHCRFTLRNALNHA 81
           L+T   FL  + +SS  +  ++ IC++T  P +CKS  P+S  ++  +  F++R +L+ +
Sbjct: 8   LITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFPHSQGNVQQYGCFSIRKSLSQS 67

Query: 82  RTFLTLID------AHLNLLPFPSALHDCRGLAEANLDFISQTFSIVNFTT-----MALP 141
           R F+  +D      AHL+      AL DCR LA   +D++  +F  VN T+       L 
Sbjct: 68  RKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTSFKPLS 127

Query: 142 YYQAHEMMSLISAMITNEDTCYEGLVGLGSVVG---LVDKVLEAISFDRKLYSLYLSLFK 201
           + +A ++ +L+SA +TNE TC EGL    S      +   V   +  D KL  + L+LF 
Sbjct: 128 FPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVSLALFT 187

Query: 202 MGWVSEDMK-------APMLPNMNHFG----VGKGQLKLKMSPKDRAYYERLVHRKKPAA 261
            GWV +  K        P   +  H         G L LKM+ K +A YE L  RK    
Sbjct: 188 KGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAVYESLSRRKLADG 247

Query: 262 ARRLLQTNYEDDGILI--------------NGNNLSDAIAAAPNKSVITKGYFLIYVTAG 321
                     DDG ++              N  N++ A+AAAPN +  + G+FLIYVTAG
Sbjct: 248 D----SNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAG 307

Query: 322 IYNESVLVPKEKRYVLMIGDGNNETIITGNKNVVDGSTTFNSATVAVEGTGFFGVNLTIS 381
           IY E + + K KRY++MIGDG N+T++TGN++VVDG TTFNSAT AV    F  VN+T  
Sbjct: 308 IYEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFR 367

Query: 382 NTAGAAKHQAVALRVSADNVTLYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAA 441
           NTAG  KHQAVALR  AD    YSC FE YQDTLYTHSLRQFYRECD+YGTVDFIFGNAA
Sbjct: 368 NTAGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAA 427

Query: 442 VVLQNCNIYARLPLSGQFNALTAQGRTDPNQNTGTSIHNCTIKPTPELAASPAT-KSYLG 501
           VV QNCN+Y R P+  QFNA+TAQGR+DPNQNTGTSI NCTIKP  +L +S  T K+YLG
Sbjct: 428 VVFQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLVSSNYTVKTYLG 487

Query: 502 RPWKEYSRTVYMQSFIGAFIDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRASWV-V 557
           RPWKEYSRTVYMQS+I  F++P GW+EW+G   L+T YYAE+NN+GPGS+T+ R +W   
Sbjct: 488 RPWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGY 547

[Close]

BLAST of Lsi10G006560 vs. ExPASy Swiss-Prot
Match: Q94CB1 (Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3702 GN=PME25 PE=2 SV=1)

HSP 1 Score: 401.4 bits (1030), Expect = 1.7e-110
Identity = 227/547 (41.50%), Postives = 320/547 (58.50%), Query Frame = 0


Query: 46  CQSTTSPSFCKSFL---PNSPASIHSHCRFTLRNALNHARTFLTLIDAHLNLLPFP---- 105
           C+ST  P  C++ L    +SP+  + + +FT++  L  A     +I ++   +       
Sbjct: 83  CKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVITSYARRVESKPGSA 142

Query: 106 -----SALHDCRGLAEANLDFISQTFSIVNFTTMALPYYQAHEMMSLISAMITNEDTCYE 165
                 A+ DC  L+E +++++ +T +    T   +       + SL+S ++TN+ TC +
Sbjct: 143 TAEEIGAVADCGELSELSVNYL-ETVTTELKTAQVMTAALVEHVNSLLSGVVTNQQTCLD 202

Query: 166 GLVGLGSVVGLVDKVLEAISFDRKLYSLYLSLFKMGWVSEDMKAPMLPNMNHFGVGKGQL 225
           GLV   S  G    +   +    +LYS+      +G VS  +      N+  F   KG++
Sbjct: 203 GLVEAKS--GFAAAIGSPMGNLTRLYSI-----SLGLVSHALNR----NLKRFKASKGKI 262

Query: 226 KLKMSPKDRAYYERLVHRKKPA---------AARRLLQTNYEDDGILI------------ 285
               +   R   E L+   +            +R L +      G ++            
Sbjct: 263 LGGGNSTYREPLETLIKGLRKTCDNDKDCRKTSRNLGELGETSGGSILVSKAVIVGPFKS 322

Query: 286 -NGNNLSDAIAAAPNKSVITKGYFLIYVTAGIYNESVLVPKEKRYVLMIGDGNNETIITG 345
            N   ++DAIAAAPN +    GYF+IY   G+Y E ++VP  K+ ++++GDG N+TIITG
Sbjct: 323 DNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTIITG 382

Query: 346 NKNVVDGSTTFNSATVAVEGTGFFGVNLTISNTAGAAKHQAVALRVSADNVTLYSCIFEG 405
           N NVVDG TT+N ++ AV G  F  V++T  NTAG  KHQAVALR +A+  + Y C FEG
Sbjct: 383 NHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCSFEG 442

Query: 406 YQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFNALTAQGRTDP 465
           YQDTLY HSLRQFYRECDIYGTVDFIFGNAA + QNCNIYAR P++ Q NA+TA GR DP
Sbjct: 443 YQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGRLDP 502

Query: 466 NQNTGTSIHNCTIKPTPELAASP-ATKSYLGRPWKEYSRTVYMQSFIGAFIDPAGWKEWD 525
           NQNTG SI NCTIK  P+LAA P +  ++LGRPWK YSRTV+MQS+I   + P GW EW+
Sbjct: 503 NQNTGISIINCTIKAAPDLAAEPKSAMTFLGRPWKPYSRTVFMQSYISDIVQPVGWLEWN 562

Query: 526 GTLNLNTSYYAEFNNSGPGSDTSRRASWV-VGVINATVANNFTVSQFLAGDQWLPPTAVP 557
           GT+ L+T YY E++N GPG++T++R  W+   ++N   A NFTV  F  GD WLP T +P
Sbjct: 563 GTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDTWLPQTDIP 617

[Close]

BLAST of Lsi10G006560 vs. ExPASy Swiss-Prot
Match: Q9FF77 (Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3702 GN=PME47 PE=2 SV=1)

HSP 1 Score: 401.0 bits (1029), Expect = 2.2e-110
Identity = 233/554 (42.06%), Postives = 323/554 (58.30%), Query Frame = 0


Query: 33  SSSSFAVVPTAEICQSTTSPSFCKSFL---PNSPASIHSHCRFTLRNALNHARTFLTLI- 92
           S S  + +P    C+ST  P  C++ L    +SP+  + + +FT++  L  AR    +I 
Sbjct: 75  SPSQPSPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVIN 134

Query: 93  --------DAHLNLLPFPSALHDCRGLAEANLDF---ISQTFSIVNFTTMALPYYQAHEM 152
                   D   + +   SA+ DC  LAE ++++   +++        T AL       +
Sbjct: 135 RFAQRVEADPGTSTVEEVSAVADCGELAELSVEYLETVTEELKAAELMTAAL----VDRV 194

Query: 153 MSLISAMITNEDTCYEGLVGLGSVVGLVDKVLEAISFDRKLYSLYLSLFKMGWVSEDMKA 212
            SL+  ++TN+ TC +GLV   S  G    +   +    +LYS+ L L     ++ ++K 
Sbjct: 195 TSLLGGVVTNQQTCLDGLVDAKS--GFATAIGTPLGNLTRLYSVSLGLVSHA-LNRNLKR 254

Query: 213 PMLPNMNHFGVGKGQLKLKMSPKDRAYYERLVHRKKPAAARRLLQTNYEDDG--ILI--- 272
                   FG G   ++  +    +   +     K    A R L    E  G  IL+   
Sbjct: 255 YKGSKGKIFGGGNKPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGGSILVREA 314

Query: 273 ---------NGNNLSDAIAAAPNKSVITKGYFLIYVTAGIYNESVLVPKEKRYVLMIGDG 332
                    N   +++A+AAAPN +   +GYF+IY  AG+Y E V++  +KR +++IGDG
Sbjct: 315 VTVGPYETDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDG 374

Query: 333 NNETIITGNKNVVDGSTTFNSATVAVEGTGFFGVNLTISNTAGAAKHQAVALRVSADNVT 392
            N+TII+GN + +DG TT+NS+T AV G  F  V++T  NTAG  KHQAVA+R +AD  T
Sbjct: 375 INKTIISGNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGST 434

Query: 393 LYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFNAL 452
            Y C FEGYQDTLY HSLRQFYRECDIYGT+DFIFGNAA + QNCNIYAR P++ Q NA+
Sbjct: 435 FYRCSFEGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAV 494

Query: 453 TAQGRTDPNQNTGTSIHNCTIKPTPELAASP-ATKSYLGRPWKEYSRTVYMQSFIGAFID 512
           TA GRTDPNQ TG SI NCTI   P+LAA P +T ++LGRPWK YSRTVY+QS+I   + 
Sbjct: 495 TAHGRTDPNQKTGISIINCTIGAAPDLAADPKSTMTFLGRPWKPYSRTVYIQSYISDVVQ 554

Query: 513 PAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRASWV-VGVINATVANNFTVSQFLAGDQ 556
           P GW EW+GT  L+T  Y E++N GPG+DTS+R  W    ++N   A NFTV  F  GD 
Sbjct: 555 PVGWLEWNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDT 614

[Close]

BLAST of Lsi10G006560 vs. ExPASy TrEMBL
Match: A0A5A7TRW1 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold299G002210 PE=3 SV=1)

HSP 1 Score: 934.9 bits (2415), Expect = 1.6e-268
Identity = 467/556 (83.99%), Postives = 501/556 (90.11%), Query Frame = 0


Query: 14  MASSNPISLLTFLTFLFLLSSSSFAVVPTAEICQSTTSPSFCKSFLPNSPASIHSHCRFT 73
           M SSNPIS+LTFLT LF LS SS A VPTAEICQSTTSP FC  FLPNSPAS+HSHCRFT
Sbjct: 1   MVSSNPISVLTFLTLLFFLSPSSSA-VPTAEICQSTTSPPFCNFFLPNSPASVHSHCRFT 60

Query: 74  LRNALNHARTFLTLIDAHLNLLPFPSALHDCRGLAEANLDFISQTFSIVNFTTMA-LPYY 133
           L +AL HARTFL+L++AHLNL P  S LHDCR LAEANLDF+SQTFSIVN TT   LPYY
Sbjct: 61  LHHALVHARTFLSLVNAHLNLFPSLSPLHDCRSLAEANLDFLSQTFSIVNSTTTTLLPYY 120

Query: 134 QAHEMMSLISAMITNEDTCYEGLVGLGSVVGLVDKVLEAISFDRKLYSLYLSLFKMGWVS 193
            AHE ++L+SA+ITNEDTCYEGL GL S VGLVDKVLEAISFD+KLYSLYLSLFKMGWVS
Sbjct: 121 DAHEKLTLMSAVITNEDTCYEGLAGLDSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVS 180

Query: 194 EDMKAPMLPNMNHFGVGKGQLKLKMSPKDRAYYERLVHRKKPAAARRLLQTNYEDDGILI 253
           +D+KAP LP M HFGVGKGQLKLKMSPKDRAYYERLVHR KP  ARRLLQTNY+DDGI+I
Sbjct: 181 KDLKAPTLPKMKHFGVGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVI 240

Query: 254 NG------------NNLSDAIAAAPNKSVITKGYFLIYVTAGIYNESVLVPKEKRYVLMI 313
           NG             N++ AIAAAPNK+ + KGYFLI+V AGIYNE+VL+PKEKRYVL+I
Sbjct: 241 NGIVGVDQNGTYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLIPKEKRYVLLI 300

Query: 314 GDGNNETIITGNKNVVDGSTTFNSATVAVEGTGFFGVNLTISNTAGAAKHQAVALRVSAD 373
           G+GNN+TIITGNKNVVDGSTTFNSATVAVEGTGF GVNLTISNTAGAAKHQAVALRVSAD
Sbjct: 301 GEGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTISNTAGAAKHQAVALRVSAD 360

Query: 374 NVTLYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQF 433
           N TLY+CIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQF
Sbjct: 361 NATLYNCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQF 420

Query: 434 NALTAQGRTDPNQNTGTSIHNCTIKPTPELAASPATKSYLGRPWKEYSRTVYMQSFIGAF 493
           NA+TAQGRTDPNQNTGTSIHNCTIK TPELAASPATKSYLGRPWK+YSRTVYMQSFI +F
Sbjct: 421 NAVTAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSF 480

Query: 494 IDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRASWVVGVINATVANNFTVSQFLAGD 553
           IDPAGWKEWDGT+NLNTSYYAEFNNSGPGSDTS+R SW VGVINATVAN+FTVSQFLAGD
Sbjct: 481 IDPAGWKEWDGTMNLNTSYYAEFNNSGPGSDTSQRVSWAVGVINATVANSFTVSQFLAGD 540

Query: 554 QWLPPTAVPYTGGLIS 557
           +WLPPT VPYTGGLIS
Sbjct: 541 RWLPPTDVPYTGGLIS 555

[Close]

BLAST of Lsi10G006560 vs. ExPASy TrEMBL
Match: A0A1S3C644 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103497470 PE=3 SV=1)

HSP 1 Score: 934.9 bits (2415), Expect = 1.6e-268
Identity = 467/556 (83.99%), Postives = 501/556 (90.11%), Query Frame = 0


Query: 14  MASSNPISLLTFLTFLFLLSSSSFAVVPTAEICQSTTSPSFCKSFLPNSPASIHSHCRFT 73
           M SSNPIS+LTFLT LF LS SS A VPTAEICQSTTSP FC  FLPNSPAS+HSHCRFT
Sbjct: 1   MVSSNPISVLTFLTLLFFLSPSSSA-VPTAEICQSTTSPPFCNFFLPNSPASVHSHCRFT 60

Query: 74  LRNALNHARTFLTLIDAHLNLLPFPSALHDCRGLAEANLDFISQTFSIVNFTTMA-LPYY 133
           L +AL HARTFL+L++AHLNL P  S LHDCR LAEANLDF+SQTFSIVN TT   LPYY
Sbjct: 61  LHHALVHARTFLSLVNAHLNLFPSLSPLHDCRSLAEANLDFLSQTFSIVNSTTTTLLPYY 120

Query: 134 QAHEMMSLISAMITNEDTCYEGLVGLGSVVGLVDKVLEAISFDRKLYSLYLSLFKMGWVS 193
            AHE ++L+SA+ITNEDTCYEGL GL S VGLVDKVLEAISFD+KLYSLYLSLFKMGWVS
Sbjct: 121 DAHEKLTLMSAVITNEDTCYEGLAGLDSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVS 180

Query: 194 EDMKAPMLPNMNHFGVGKGQLKLKMSPKDRAYYERLVHRKKPAAARRLLQTNYEDDGILI 253
           +D+KAP LP M HFGVGKGQLKLKMSPKDRAYYERLVHR KP  ARRLLQTNY+DDGI+I
Sbjct: 181 KDLKAPTLPKMKHFGVGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVI 240

Query: 254 NG------------NNLSDAIAAAPNKSVITKGYFLIYVTAGIYNESVLVPKEKRYVLMI 313
           NG             N++ AIAAAPNK+ + KGYFLI+V AGIYNE+VL+PKEKRYVL+I
Sbjct: 241 NGIVGVDQNGTYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLIPKEKRYVLLI 300

Query: 314 GDGNNETIITGNKNVVDGSTTFNSATVAVEGTGFFGVNLTISNTAGAAKHQAVALRVSAD 373
           G+GNN+TIITGNKNVVDGSTTFNSATVAVEGTGF GVNLTISNTAGAAKHQAVALRVSAD
Sbjct: 301 GEGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTISNTAGAAKHQAVALRVSAD 360

Query: 374 NVTLYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQF 433
           N TLY+CIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQF
Sbjct: 361 NATLYNCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQF 420

Query: 434 NALTAQGRTDPNQNTGTSIHNCTIKPTPELAASPATKSYLGRPWKEYSRTVYMQSFIGAF 493
           NA+TAQGRTDPNQNTGTSIHNCTIK TPELAASPATKSYLGRPWK+YSRTVYMQSFI +F
Sbjct: 421 NAVTAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSF 480

Query: 494 IDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRASWVVGVINATVANNFTVSQFLAGD 553
           IDPAGWKEWDGT+NLNTSYYAEFNNSGPGSDTS+R SW VGVINATVAN+FTVSQFLAGD
Sbjct: 481 IDPAGWKEWDGTMNLNTSYYAEFNNSGPGSDTSQRVSWAVGVINATVANSFTVSQFLAGD 540

Query: 554 QWLPPTAVPYTGGLIS 557
           +WLPPT VPYTGGLIS
Sbjct: 541 RWLPPTDVPYTGGLIS 555

[Close]

BLAST of Lsi10G006560 vs. ExPASy TrEMBL
Match: A0A6J1EJM8 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111434929 PE=3 SV=1)

HSP 1 Score: 868.2 bits (2242), Expect = 1.8e-248
Identity = 439/554 (79.24%), Postives = 475/554 (85.74%), Query Frame = 0


Query: 15  ASSNPISLLTFLTFLFLLSSSSFAVVPTAEICQSTTSPSFCKSFLPNSPASIHSHCRFTL 74
           +SSNPISLL F + LFL +SSS   VPT E+CQS TSPSFCKSFLP+SPAS+HSHCR+TL
Sbjct: 3   SSSNPISLLLFFSLLFLFTSSS--AVPTVEVCQSATSPSFCKSFLPSSPASVHSHCRYTL 62

Query: 75  RNALNHARTFLTLIDAHLNLLPFPSALHDCRGLAEANLDFISQTFSIVNFTTMALPYYQA 134
            +AL  ARTF+TL+D  L L P  SALHDCR LA ANLDFIS T   VN TT ALP+YQA
Sbjct: 63  HHALVQARTFVTLVDLQLTLFPSLSALHDCRSLAVANLDFISSTVDTVNTTTNALPFYQA 122

Query: 135 HEMMSLISAMITNEDTCYEGLVGLGSVVGLVDKVLEAISFDRKLYSLYLSLFKMGWVSED 194
            EM+SL+SA+ITN DTC++GL G G  +G VDKV++AISFDR+LYSLYLSLFKMGWVS++
Sbjct: 123 DEMISLLSAVITNIDTCHDGLAGSGLGLGFVDKVVKAISFDRQLYSLYLSLFKMGWVSKN 182

Query: 195 MKAPMLPNMNHFGVGKGQLKLKMSPKDRAYYERLVHRKKPAAARRLLQTNYEDDGILING 254
           MKAP LP  NHFG GKG LKLKMSPKDRAYYERLVH KKP AARRLLQTNY DDGIL+NG
Sbjct: 183 MKAPKLPKTNHFGFGKGPLKLKMSPKDRAYYERLVHGKKP-AARRLLQTNYADDGILVNG 242

Query: 255 ------------NNLSDAIAAAPNKSVITKGYFLIYVTAGIYNESVLVPKEKRYVLMIGD 314
                         ++DAIAAAPNKS    GYFLI+VTAGIY ESV+VPK K+YVLMIG+
Sbjct: 243 VVAVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESVVVPKSKKYVLMIGE 302

Query: 315 GNNETIITGNKNVVDGSTTFNSATVAVEGTGFFGVNLTISNTAGAAKHQAVALRVSADNV 374
           GNN TIITGNKNVVDGSTTFNSATV VEGTGFFG NLTI+NTAGA KHQAVALRVSAD  
Sbjct: 303 GNNRTIITGNKNVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAIKHQAVALRVSADMT 362

Query: 375 TLYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFNA 434
           T YSCIFEGYQDTLY HSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFNA
Sbjct: 363 TFYSCIFEGYQDTLYAHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFNA 422

Query: 435 LTAQGRTDPNQNTGTSIHNCTIKPTPELAASPATKSYLGRPWKEYSRTVYMQSFIGAFID 494
           LTAQGRTD NQNTG  IH CTIKPTP+LAASP TKSYLGRPWKE+SRTVYMQSFI AFID
Sbjct: 423 LTAQGRTDQNQNTGIFIHKCTIKPTPDLAASPTTKSYLGRPWKEFSRTVYMQSFIDAFID 482

Query: 495 PAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRASWVVGVINATVANNFTVSQFLAGDQW 554
           PAGWKEWDGTLNLNTSYYAEF+NSGPGSDTS+RA W VGVINATVAN FTVS+ LAGDQW
Sbjct: 483 PAGWKEWDGTLNLNTSYYAEFDNSGPGSDTSQRAQWAVGVINATVANIFTVSEILAGDQW 542

Query: 555 LPPTAVPYTGGLIS 557
           LPPTAVPY+GGLIS
Sbjct: 543 LPPTAVPYSGGLIS 553

[Close]

BLAST of Lsi10G006560 vs. ExPASy TrEMBL
Match: A0A6J1I6B8 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111470332 PE=3 SV=1)

HSP 1 Score: 865.1 bits (2234), Expect = 1.5e-247
Identity = 438/554 (79.06%), Postives = 475/554 (85.74%), Query Frame = 0


Query: 15  ASSNPISLLTFLTFLFLLSSSSFAVVPTAEICQSTTSPSFCKSFLPNSPASIHSHCRFTL 74
           +SSNPISLLTF + LFL   +S + VPT E+CQS TSPSFCKSFLP+SPAS+HSHCR+TL
Sbjct: 3   SSSNPISLLTFFSLLFLFLFTSSSAVPTVEVCQSATSPSFCKSFLPSSPASVHSHCRYTL 62

Query: 75  RNALNHARTFLTLIDAHLNLLPFPSALHDCRGLAEANLDFISQTFSIVNFTTMALPYYQA 134
            +AL  ARTF+TL+D  L LLP  SALHDCR LA ANLDFIS T + VN TT ALP+YQA
Sbjct: 63  HHALVQARTFITLVDLQLTLLPSFSALHDCRSLAMANLDFISSTVNTVNTTTNALPFYQA 122

Query: 135 HEMMSLISAMITNEDTCYEGLVGLGSVVGLVDKVLEAISFDRKLYSLYLSLFKMGWVSED 194
            EM+SL+SA+ITN DTC++GL G G  +GLVDKV++AISFDR+LYSLYLSLFKMGWVS+ 
Sbjct: 123 DEMISLLSAVITNVDTCHDGLAGSG--LGLVDKVVKAISFDRQLYSLYLSLFKMGWVSKT 182

Query: 195 MKAPMLPNMNHFGVGKGQLKLKMSPKDRAYYERLVHRKKPAAARRLLQTNYEDDGILING 254
           MKAP LP  NHFG G G LKLKMSPKDRAYYERL+H KKP AARRLLQTN  DDGIL+NG
Sbjct: 183 MKAPKLPKTNHFGFGNGPLKLKMSPKDRAYYERLMHGKKP-AARRLLQTNNADDGILVNG 242

Query: 255 ------------NNLSDAIAAAPNKSVITKGYFLIYVTAGIYNESVLVPKEKRYVLMIGD 314
                         ++DAIAAAPNKS    GYFLI+VTAGIY ESV+VPK K+YVLMIG+
Sbjct: 243 VVAVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESVVVPKSKKYVLMIGE 302

Query: 315 GNNETIITGNKNVVDGSTTFNSATVAVEGTGFFGVNLTISNTAGAAKHQAVALRVSADNV 374
           GNN TIITGNKNVVDGSTTFNSATV VEGTGFFG NLTI+NTAGA KHQAVALRVSAD  
Sbjct: 303 GNNRTIITGNKNVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAIKHQAVALRVSADMT 362

Query: 375 TLYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFNA 434
           T YSCIFEGYQDTLY HSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFNA
Sbjct: 363 TFYSCIFEGYQDTLYAHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFNA 422

Query: 435 LTAQGRTDPNQNTGTSIHNCTIKPTPELAASPATKSYLGRPWKEYSRTVYMQSFIGAFID 494
           LTAQGRTD NQNTG  IH CTIKPTP+LAASP TKSYLGRPWKE+SRTVYMQSFI AFID
Sbjct: 423 LTAQGRTDQNQNTGIFIHKCTIKPTPDLAASPTTKSYLGRPWKEFSRTVYMQSFIDAFID 482

Query: 495 PAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRASWVVGVINATVANNFTVSQFLAGDQW 554
           PAGWKEWDGTLNLNTSYYAEF+NSGPGSDTSRRA W VGVINATVAN FTVS+ LAGDQW
Sbjct: 483 PAGWKEWDGTLNLNTSYYAEFDNSGPGSDTSRRAQWAVGVINATVANIFTVSEILAGDQW 542

Query: 555 LPPTAVPYTGGLIS 557
           LPPTAVPY+GGLIS
Sbjct: 543 LPPTAVPYSGGLIS 553

[Close]

BLAST of Lsi10G006560 vs. ExPASy TrEMBL
Match: A0A6J1L6X0 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111499728 PE=3 SV=1)

HSP 1 Score: 829.3 bits (2141), Expect = 9.4e-237
Identity = 423/563 (75.13%), Postives = 463/563 (82.24%), Query Frame = 0


Query: 14  MASSNPISLLTFLTFLFLLSSSSFAV---VPTAEICQSTTSPSFCKSFLPNSPASIHSHC 73
           M SS+ I LLTFLT L+L S SS A+   VP A+IC STTSPSFCK+FLPNS A++HSHC
Sbjct: 1   MGSSSAIPLLTFLTLLYLFSFSSSAIHSPVPAADICHSTTSPSFCKAFLPNSLATVHSHC 60

Query: 74  RFTLRNALNHARTFLTLIDAHLNLLPFPSALHDCRGLAEANLDFISQTFSIVNFTTM--- 133
           RFTLR+AL HARTFL L+D HL L P  +ALHDCR LA ANLDF+S TF  VN TT    
Sbjct: 61  RFTLRHALAHARTFLALVDTHLTLFPSLAALHDCRSLAAANLDFLSNTFPTVNTTTTTTT 120

Query: 134 -ALPYYQAHEMMSLISAMITNEDTCYEGLVGLGSVVGLVDKVLEAISFDRKLYSLYLSLF 193
            ALP+Y+A EM+SL+SA+ITNE+TCYEGL+  GS  GLVDKVL+AISFD KLYSLYLSLF
Sbjct: 121 NALPFYKAEEMVSLLSAVITNENTCYEGLMSSGSASGLVDKVLKAISFDTKLYSLYLSLF 180

Query: 194 KMGWVSEDMKAPMLPNMNHFGVGKGQLKLKMSPKDRAYYERLVHRKKPAAARRLLQTN-Y 253
           KMGWVS D+KAP +P M HFG GKGQLKL MSPK+RAYYERL+HR KP  ARRLLQTN  
Sbjct: 181 KMGWVSNDVKAPHVPKMKHFGRGKGQLKLNMSPKNRAYYERLMHRSKP-PARRLLQTNSN 240

Query: 254 EDDGILING------------NNLSDAIAAAPNKSVITKGYFLIYVTAGIYNESVLVPKE 313
           EDDGIL+NG              ++ AI AAPN SV   GYFLI VTAG+YNESV+VPK 
Sbjct: 241 EDDGILVNGIVAVDQNGTYDFTTINAAIQAAPNNSVAADGYFLIIVTAGVYNESVMVPKH 300

Query: 314 KRYVLMIGDGNNETIITGNKNVVDGSTTFNSATVAVEGTGFFGVNLTISNTAGAAKHQAV 373
           K+YVLMIG+GNN+TIITGNKNVVDGSTTFNS T  VEGTGFFGVNLTI+NTAG  K QAV
Sbjct: 301 KKYVLMIGEGNNQTIITGNKNVVDGSTTFNSGTFIVEGTGFFGVNLTITNTAGPNKFQAV 360

Query: 374 ALRVSADNVTLYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYAR 433
           ALRVSAD  T YSCIFEGYQDTLY HSLRQFYRECDIYGT+DFI G+AAVV QNCNIYAR
Sbjct: 361 ALRVSADMTTFYSCIFEGYQDTLYPHSLRQFYRECDIYGTIDFILGDAAVVFQNCNIYAR 420

Query: 434 LPLSGQFNALTAQGRTDPNQNTGTSIHNCTIKPTPELAASPATKSYLGRPWKEYSRTVYM 493
           LP+ GQ+  +TAQGRTD NQNTG SIHNCTIKPT +LAASP  KSY GRPWKEY+RTVYM
Sbjct: 421 LPMKGQYIVITAQGRTDRNQNTGISIHNCTIKPTAQLAASPTIKSYFGRPWKEYARTVYM 480

Query: 494 QSFIGAFIDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRASWVVGVINATVANNFTV 553
           QSFI AFIDP GW  WDGTLNLNTSYYAEFNNSGPGSDTSRRA W VG+INATVANNFTV
Sbjct: 481 QSFIDAFIDPEGWNVWDGTLNLNTSYYAEFNNSGPGSDTSRRAKWAVGLINATVANNFTV 540

Query: 554 SQFLAGDQWLPPTAVPYTGGLIS 557
           S FL GDQWLPPT VPYTGGLIS
Sbjct: 541 SPFLGGDQWLPPTTVPYTGGLIS 562

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BLAST of Lsi10G006560 vs. NCBI nr
Match: XP_038902853.1 (probable pectinesterase/pectinesterase inhibitor 20 [Benincasa hispida])

HSP 1 Score: 976.1 bits (2522), Expect = 1.3e-280
Identity = 485/555 (87.39%), Postives = 515/555 (92.79%), Query Frame = 0


Query: 14  MASSNPISLLTFLTFLFLLSSSSFAVVPTAEICQSTTSPSFCKSFLPNSPASIHSHCRFT 73
           M SSN ISLLTFLTFLFLL SS  AVVPT EIC+STT PSFC +FLPNSPAS+HSHCRFT
Sbjct: 1   MVSSNSISLLTFLTFLFLLPSSFSAVVPTTEICRSTTFPSFCNAFLPNSPASVHSHCRFT 60

Query: 74  LRNALNHARTFLTLIDAHLNLLPFPSALHDCRGLAEANLDFISQTFSIVNFTTMALPYYQ 133
           L +AL HA+TFL L+DAHL+L PFPSALHDCR LAEANLD+I+QTFSIVN T++ALPYYQ
Sbjct: 61  LHHALTHAQTFLALVDAHLSLFPFPSALHDCRSLAEANLDYITQTFSIVNSTSVALPYYQ 120

Query: 134 AHEMMSLISAMITNEDTCYEGLVGLGSVVGLVDKVLEAISFDRKLYSLYLSLFKMGWVSE 193
           AHEMMSL+SAMITNE+TCYEGL GLGS +GLVDKVLEAISFDRKLYSLYLSLFKMGWVS+
Sbjct: 121 AHEMMSLMSAMITNENTCYEGLAGLGSAIGLVDKVLEAISFDRKLYSLYLSLFKMGWVSK 180

Query: 194 DMKAPMLPNMNHFGVGKGQLKLKMSPKDRAYYERLVHRKKPAAARRLLQTNYEDDGILIN 253
           +MKAP+LP MNHFGVGKGQLKLKMSPKDRAYYERLVH KKPAAARRLLQTNYE+DGIL+N
Sbjct: 181 NMKAPILPKMNHFGVGKGQLKLKMSPKDRAYYERLVHSKKPAAARRLLQTNYENDGILVN 240

Query: 254 G------------NNLSDAIAAAPNKSVITKGYFLIYVTAGIYNESVLVPKEKRYVLMIG 313
           G             N+S AIAAAP+K+ +TKGYFLI+VTAGIYNE+VLVPK KRYVL+IG
Sbjct: 241 GVVAVDQNGTYDFTNISAAIAAAPDKTTVTKGYFLIFVTAGIYNETVLVPKNKRYVLLIG 300

Query: 314 DGNNETIITGNKNVVDGSTTFNSATVAVEGTGFFGVNLTISNTAGAAKHQAVALRVSADN 373
           +GNN+TII GNKNVVDGSTTFNSATVAVEGTGFFGVNLTISNTAG AKHQAVALRVSADN
Sbjct: 301 EGNNQTIINGNKNVVDGSTTFNSATVAVEGTGFFGVNLTISNTAGPAKHQAVALRVSADN 360

Query: 374 VTLYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFN 433
            TLYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFN
Sbjct: 361 ATLYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFN 420

Query: 434 ALTAQGRTDPNQNTGTSIHNCTIKPTPELAASPATKSYLGRPWKEYSRTVYMQSFIGAFI 493
           ALTAQGRTDPNQNTGTSIHNCTIK TPELAA+PATKSYLGRPWKEYSRTVYMQSFI AFI
Sbjct: 421 ALTAQGRTDPNQNTGTSIHNCTIKATPELAANPATKSYLGRPWKEYSRTVYMQSFIDAFI 480

Query: 494 DPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRASWVVGVINATVANNFTVSQFLAGDQ 553
           DPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRA WVVGVINATVAN+FTVSQFLAGDQ
Sbjct: 481 DPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRAPWVVGVINATVANSFTVSQFLAGDQ 540

Query: 554 WLPPTAVPYTGGLIS 557
           WLPPTAVPYTGGLIS
Sbjct: 541 WLPPTAVPYTGGLIS 555

[Close]

BLAST of Lsi10G006560 vs. NCBI nr
Match: XP_008457895.1 (PREDICTED: pectinesterase-like [Cucumis melo] >KAA0045890.1 pectinesterase-like [Cucumis melo var. makuwa] >TYK13697.1 pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 934.9 bits (2415), Expect = 3.3e-268
Identity = 467/556 (83.99%), Postives = 501/556 (90.11%), Query Frame = 0


Query: 14  MASSNPISLLTFLTFLFLLSSSSFAVVPTAEICQSTTSPSFCKSFLPNSPASIHSHCRFT 73
           M SSNPIS+LTFLT LF LS SS A VPTAEICQSTTSP FC  FLPNSPAS+HSHCRFT
Sbjct: 1   MVSSNPISVLTFLTLLFFLSPSSSA-VPTAEICQSTTSPPFCNFFLPNSPASVHSHCRFT 60

Query: 74  LRNALNHARTFLTLIDAHLNLLPFPSALHDCRGLAEANLDFISQTFSIVNFTTMA-LPYY 133
           L +AL HARTFL+L++AHLNL P  S LHDCR LAEANLDF+SQTFSIVN TT   LPYY
Sbjct: 61  LHHALVHARTFLSLVNAHLNLFPSLSPLHDCRSLAEANLDFLSQTFSIVNSTTTTLLPYY 120

Query: 134 QAHEMMSLISAMITNEDTCYEGLVGLGSVVGLVDKVLEAISFDRKLYSLYLSLFKMGWVS 193
            AHE ++L+SA+ITNEDTCYEGL GL S VGLVDKVLEAISFD+KLYSLYLSLFKMGWVS
Sbjct: 121 DAHEKLTLMSAVITNEDTCYEGLAGLDSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVS 180

Query: 194 EDMKAPMLPNMNHFGVGKGQLKLKMSPKDRAYYERLVHRKKPAAARRLLQTNYEDDGILI 253
           +D+KAP LP M HFGVGKGQLKLKMSPKDRAYYERLVHR KP  ARRLLQTNY+DDGI+I
Sbjct: 181 KDLKAPTLPKMKHFGVGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVI 240

Query: 254 NG------------NNLSDAIAAAPNKSVITKGYFLIYVTAGIYNESVLVPKEKRYVLMI 313
           NG             N++ AIAAAPNK+ + KGYFLI+V AGIYNE+VL+PKEKRYVL+I
Sbjct: 241 NGIVGVDQNGTYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLIPKEKRYVLLI 300

Query: 314 GDGNNETIITGNKNVVDGSTTFNSATVAVEGTGFFGVNLTISNTAGAAKHQAVALRVSAD 373
           G+GNN+TIITGNKNVVDGSTTFNSATVAVEGTGF GVNLTISNTAGAAKHQAVALRVSAD
Sbjct: 301 GEGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTISNTAGAAKHQAVALRVSAD 360

Query: 374 NVTLYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQF 433
           N TLY+CIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQF
Sbjct: 361 NATLYNCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQF 420

Query: 434 NALTAQGRTDPNQNTGTSIHNCTIKPTPELAASPATKSYLGRPWKEYSRTVYMQSFIGAF 493
           NA+TAQGRTDPNQNTGTSIHNCTIK TPELAASPATKSYLGRPWK+YSRTVYMQSFI +F
Sbjct: 421 NAVTAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSF 480

Query: 494 IDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRASWVVGVINATVANNFTVSQFLAGD 553
           IDPAGWKEWDGT+NLNTSYYAEFNNSGPGSDTS+R SW VGVINATVAN+FTVSQFLAGD
Sbjct: 481 IDPAGWKEWDGTMNLNTSYYAEFNNSGPGSDTSQRVSWAVGVINATVANSFTVSQFLAGD 540

Query: 554 QWLPPTAVPYTGGLIS 557
           +WLPPT VPYTGGLIS
Sbjct: 541 RWLPPTDVPYTGGLIS 555

[Close]

BLAST of Lsi10G006560 vs. NCBI nr
Match: XP_004148470.1 (pectinesterase [Cucumis sativus] >KAE8651957.1 hypothetical protein Csa_006047 [Cucumis sativus])

HSP 1 Score: 929.9 bits (2402), Expect = 1.1e-266
Identity = 464/555 (83.60%), Postives = 496/555 (89.37%), Query Frame = 0


Query: 14  MASSNPISLLTFLTFLFLLSSSSFAVVPTAEICQSTTSPSFCKSFLPNSPASIHSHCRFT 73
           M SSNPISLLTFLT LFLLS SS A  PTA+ICQSTTSP FC SFLP SP SIHSHCRFT
Sbjct: 1   MVSSNPISLLTFLTLLFLLSPSSSA-APTADICQSTTSPPFCNSFLPKSPDSIHSHCRFT 60

Query: 74  LRNALNHARTFLTLIDAHLNLLPFPSALHDCRGLAEANLDFISQTFSIVNFTTMALPYYQ 133
           L  AL HARTFL+L++A LNLLP  SALHDCR LAEANLDF+ QTFSIVN TT  LPYY 
Sbjct: 61  LHQALAHARTFLSLVNAQLNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYD 120

Query: 134 AHEMMSLISAMITNEDTCYEGLVGLGSVVGLVDKVLEAISFDRKLYSLYLSLFKMGWVSE 193
           AHEM+SLISA+ITN DTCYEGL  L S VGLVDKVLEAISFD+KLYSLYLSLFKMGWVS+
Sbjct: 121 AHEMLSLISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSK 180

Query: 194 DMKAPMLPNMNHFGVGKGQLKLKMSPKDRAYYERLVHRKKPAAARRLLQTNYEDDGILIN 253
           D+KAP  P MNHFG GKGQLKLKMSPKDRAYYERLVHR KP  ARRLLQTNY+DDGI++N
Sbjct: 181 DLKAPTFPKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVVN 240

Query: 254 G------------NNLSDAIAAAPNKSVITKGYFLIYVTAGIYNESVLVPKEKRYVLMIG 313
           G             N++ AIAAAPNK+ + KGYFLI+V AGIYNE+VLVPKEKRYVL+IG
Sbjct: 241 GIVGVDQNGMYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLVPKEKRYVLLIG 300

Query: 314 DGNNETIITGNKNVVDGSTTFNSATVAVEGTGFFGVNLTISNTAGAAKHQAVALRVSADN 373
           +GNN+TIITGNKNVVDGSTTFNSATVAVEGTGF GVNLTI+NTAG+AKHQAVALRVSADN
Sbjct: 301 EGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTITNTAGSAKHQAVALRVSADN 360

Query: 374 VTLYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFN 433
           VTLY+CIFEGYQDTLYTHSLRQFYRECD+YGTVDFIFGNAAVVLQNCNIYARLP+SGQFN
Sbjct: 361 VTLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSGQFN 420

Query: 434 ALTAQGRTDPNQNTGTSIHNCTIKPTPELAASPATKSYLGRPWKEYSRTVYMQSFIGAFI 493
           ALTAQGRTDPNQNTGTSIHNCTIK TPELAASPATKSYLGRPWK+YSRTVYMQSFI +FI
Sbjct: 421 ALTAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFI 480

Query: 494 DPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRASWVVGVINATVANNFTVSQFLAGDQ 553
           DP GWKEWDGTLNLNTSYYAEFNNSGPG DTS+RASW VGVINATVA+NFTVSQ LAGD+
Sbjct: 481 DPVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASWAVGVINATVASNFTVSQLLAGDK 540

Query: 554 WLPPTAVPYTGGLIS 557
           WLPPT VPYTGGLIS
Sbjct: 541 WLPPTEVPYTGGLIS 554

[Close]

BLAST of Lsi10G006560 vs. NCBI nr
Match: KAG6571391.1 (putative pectinesterase/pectinesterase inhibitor 41, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 873.2 bits (2255), Expect = 1.2e-249
Identity = 444/556 (79.86%), Postives = 479/556 (86.15%), Query Frame = 0


Query: 15  ASSNPISLLTFLTFLFLLSSSSFAVVPTAEICQSTTSPSFCKSFLPNSPASIHSHCRFTL 74
           +SSNPISLL F + LFL +SSS   VPT E+CQS TSPSFCKSFLP+SPAS+HSHCR+TL
Sbjct: 3   SSSNPISLLLFFSLLFLFTSSS--AVPTVEVCQSATSPSFCKSFLPSSPASVHSHCRYTL 62

Query: 75  RNALNHARTFLTLIDAHLNLLPFPSALHDCRGLAEANLDFISQTFSIVNFTTMALPYYQA 134
            +AL  ARTF+TL+D  L L P  SALHDCR LAEANLDFIS T   VN TT ALP+YQA
Sbjct: 63  HHALVQARTFVTLVDLQLTLFPSLSALHDCRSLAEANLDFISSTVDTVNTTTNALPFYQA 122

Query: 135 HEMMSLISAMITNEDTCYEGLV--GLGSVVGLVDKVLEAISFDRKLYSLYLSLFKMGWVS 194
            EM+SL+SA+ITN DTC++GL   G GS +GLVDKV++AISFDR+LYSLYLSLFKMGWVS
Sbjct: 123 DEMISLLSAVITNVDTCHDGLAGSGSGSGLGLVDKVVKAISFDRQLYSLYLSLFKMGWVS 182

Query: 195 EDMKAPMLPNMNHFGVGKGQLKLKMSPKDRAYYERLVHRKKPAAARRLLQTNYEDDGILI 254
           ++MKAP LP  NHFG G G LKLKMSPKDRAYYERLVH KKP AARRLLQTNY DDGIL+
Sbjct: 183 KNMKAPKLPKTNHFGFGNGPLKLKMSPKDRAYYERLVHGKKP-AARRLLQTNYADDGILV 242

Query: 255 NG------------NNLSDAIAAAPNKSVITKGYFLIYVTAGIYNESVLVPKEKRYVLMI 314
           NG              ++DAIAAAPNKS    GYFLI+VTAGIY ESV+VPK K+YVLMI
Sbjct: 243 NGVVAVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESVVVPKSKKYVLMI 302

Query: 315 GDGNNETIITGNKNVVDGSTTFNSATVAVEGTGFFGVNLTISNTAGAAKHQAVALRVSAD 374
           G+GNN TIITGNKNVVDGSTTFNSATV VEGTGFFG NLTI+NTAGA KHQAVALRVSAD
Sbjct: 303 GEGNNRTIITGNKNVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAIKHQAVALRVSAD 362

Query: 375 NVTLYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQF 434
             T YSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQF
Sbjct: 363 MTTFYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQF 422

Query: 435 NALTAQGRTDPNQNTGTSIHNCTIKPTPELAASPATKSYLGRPWKEYSRTVYMQSFIGAF 494
           NALTAQGRTD NQNTG  IH CTIKPTP+LAASPATKSYLGRPWKE+SRTVYMQSFI AF
Sbjct: 423 NALTAQGRTDQNQNTGIFIHKCTIKPTPDLAASPATKSYLGRPWKEFSRTVYMQSFIDAF 482

Query: 495 IDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRASWVVGVINATVANNFTVSQFLAGD 554
           IDPAGWKEWDGTLNLNTSYYAEF+NSGPGSDTSRRA W VGVINATVAN FTVS+ LAGD
Sbjct: 483 IDPAGWKEWDGTLNLNTSYYAEFDNSGPGSDTSRRAQWAVGVINATVANIFTVSEILAGD 542

Query: 555 QWLPPTAVPYTGGLIS 557
           QWLPPTAVPY+GGLIS
Sbjct: 543 QWLPPTAVPYSGGLIS 555

[Close]

BLAST of Lsi10G006560 vs. NCBI nr
Match: XP_022928019.1 (pectinesterase-like [Cucurbita moschata])

HSP 1 Score: 868.2 bits (2242), Expect = 3.8e-248
Identity = 439/554 (79.24%), Postives = 475/554 (85.74%), Query Frame = 0


Query: 15  ASSNPISLLTFLTFLFLLSSSSFAVVPTAEICQSTTSPSFCKSFLPNSPASIHSHCRFTL 74
           +SSNPISLL F + LFL +SSS   VPT E+CQS TSPSFCKSFLP+SPAS+HSHCR+TL
Sbjct: 3   SSSNPISLLLFFSLLFLFTSSS--AVPTVEVCQSATSPSFCKSFLPSSPASVHSHCRYTL 62

Query: 75  RNALNHARTFLTLIDAHLNLLPFPSALHDCRGLAEANLDFISQTFSIVNFTTMALPYYQA 134
            +AL  ARTF+TL+D  L L P  SALHDCR LA ANLDFIS T   VN TT ALP+YQA
Sbjct: 63  HHALVQARTFVTLVDLQLTLFPSLSALHDCRSLAVANLDFISSTVDTVNTTTNALPFYQA 122

Query: 135 HEMMSLISAMITNEDTCYEGLVGLGSVVGLVDKVLEAISFDRKLYSLYLSLFKMGWVSED 194
            EM+SL+SA+ITN DTC++GL G G  +G VDKV++AISFDR+LYSLYLSLFKMGWVS++
Sbjct: 123 DEMISLLSAVITNIDTCHDGLAGSGLGLGFVDKVVKAISFDRQLYSLYLSLFKMGWVSKN 182

Query: 195 MKAPMLPNMNHFGVGKGQLKLKMSPKDRAYYERLVHRKKPAAARRLLQTNYEDDGILING 254
           MKAP LP  NHFG GKG LKLKMSPKDRAYYERLVH KKP AARRLLQTNY DDGIL+NG
Sbjct: 183 MKAPKLPKTNHFGFGKGPLKLKMSPKDRAYYERLVHGKKP-AARRLLQTNYADDGILVNG 242

Query: 255 ------------NNLSDAIAAAPNKSVITKGYFLIYVTAGIYNESVLVPKEKRYVLMIGD 314
                         ++DAIAAAPNKS    GYFLI+VTAGIY ESV+VPK K+YVLMIG+
Sbjct: 243 VVAVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESVVVPKSKKYVLMIGE 302

Query: 315 GNNETIITGNKNVVDGSTTFNSATVAVEGTGFFGVNLTISNTAGAAKHQAVALRVSADNV 374
           GNN TIITGNKNVVDGSTTFNSATV VEGTGFFG NLTI+NTAGA KHQAVALRVSAD  
Sbjct: 303 GNNRTIITGNKNVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAIKHQAVALRVSADMT 362

Query: 375 TLYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFNA 434
           T YSCIFEGYQDTLY HSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFNA
Sbjct: 363 TFYSCIFEGYQDTLYAHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFNA 422

Query: 435 LTAQGRTDPNQNTGTSIHNCTIKPTPELAASPATKSYLGRPWKEYSRTVYMQSFIGAFID 494
           LTAQGRTD NQNTG  IH CTIKPTP+LAASP TKSYLGRPWKE+SRTVYMQSFI AFID
Sbjct: 423 LTAQGRTDQNQNTGIFIHKCTIKPTPDLAASPTTKSYLGRPWKEFSRTVYMQSFIDAFID 482

Query: 495 PAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRASWVVGVINATVANNFTVSQFLAGDQW 554
           PAGWKEWDGTLNLNTSYYAEF+NSGPGSDTS+RA W VGVINATVAN FTVS+ LAGDQW
Sbjct: 483 PAGWKEWDGTLNLNTSYYAEFDNSGPGSDTSQRAQWAVGVINATVANIFTVSEILAGDQW 542

Query: 555 LPPTAVPYTGGLIS 557
           LPPTAVPY+GGLIS
Sbjct: 543 LPPTAVPYSGGLIS 553

[Close]

BLAST of Lsi10G006560 vs. TAIR 10
Match: AT2G47550.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 509.6 bits (1311), Expect = 3.2e-144
Identity = 275/531 (51.79%), Postives = 349/531 (65.73%), Query Frame = 0


Query: 43  AEICQSTTSPSFCKSFLPNSPASIHSHCRFTLRNALNHARTFLTLIDAHLN------LLP 102
           + IC+    PS+C+S LPN P  I+S+ R +LR +L+ AR F+++IDA L+         
Sbjct: 36  SNICRFAPDPSYCRSVLPNQPGDIYSYGRLSLRRSLSRARRFISMIDAELDRKGKVAAKS 95

Query: 103 FPSALHDCRGLAEANLDFISQTFSIVNFTTMALPYYQAHEMMSLISAMITNEDTCYEGLV 162
              AL DC+ LA   +D++  +    + +T  L   +A ++ + +SA ITNE TC EGL 
Sbjct: 96  TVGALEDCKFLASLTMDYLLSSSQTAD-STKTLSLSRAEDVHTFLSAAITNEQTCLEGLK 155

Query: 163 GLGSVVGLVDKVLEAISFDRKLYSLYLSLFKMGWV-----SEDMKAPMLPNMNHFGVGKG 222
              S  GL   +      D KLY + L+LF  GWV     S  +  P       FG   G
Sbjct: 156 STASENGLSGDLFN----DTKLYGVSLALFSKGWVPRRQRSRPIWQPQARFKKFFGFRNG 215

Query: 223 QLKLKMSPKDRAYYERLVHRKKPAAARRLLQTNYEDDGILING----NNLSDAIAAAPNK 282
           +L LKM+ + RA Y  +  RK   +    +Q + +   ++ NG      ++ AIAAAPNK
Sbjct: 216 KLPLKMTERARAVYNTVTRRKLLQSDADAVQVS-DIVTVIQNGTGNFTTINAAIAAAPNK 275

Query: 283 SVITKGYFLIYVTAGIYNESVLVPKEKRYVLMIGDGNNETIITGNKNVVDGSTTFNSATV 342
           +  + GYFLIYVTAG+Y E V VPK KRYV+MIGDG N+T+ITGN++VVDG TTFNSAT 
Sbjct: 276 TDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGINQTVITGNRSVVDGWTTFNSATF 335

Query: 343 AVEGTGFFGVNLTISNTAGAAKHQAVALRVSADNVTLYSCIFEGYQDTLYTHSLRQFYRE 402
            + G  F GVN+TI NTAG  K QAVALR   D    YSC FE YQDTLYTHSLRQFYRE
Sbjct: 336 ILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSVFYSCSFEAYQDTLYTHSLRQFYRE 395

Query: 403 CDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFNALTAQGRTDPNQNTGTSIHNCTIKPT 462
           CD+YGTVDFIFGNAAVVLQNCN+Y R P  GQ N +TAQGRTDPNQNTGT+IH CTI+P 
Sbjct: 396 CDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEVTAQGRTDPNQNTGTAIHGCTIRPA 455

Query: 463 PELAASPAT-KSYLGRPWKEYSRTVYMQSFIGAFIDPAGWKEWDGTLNLNTSYYAEFNNS 522
            +LA S  T K+YLGRPWKEYSRTV MQ++I  F++P+GW  W G   L+T YYAE+NN+
Sbjct: 456 DDLATSNYTVKTYLGRPWKEYSRTVVMQTYIDGFLEPSGWNAWSGDFALSTLYYAEYNNT 515

Query: 523 GPGSDTSRRASWV-VGVINATVANNFTVSQFLAGDQWLPPTAVPYTGGLIS 557
           GPGSDT+ R +W    VINAT A+NFTV+ FL G+ W+  T VP+ GGLI+
Sbjct: 516 GPGSDTTNRVTWPGYHVINATDASNFTVTNFLVGEGWIGQTGVPFVGGLIA 560

[Close]

BLAST of Lsi10G006560 vs. TAIR 10
Match: AT4G02330.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 493.8 bits (1270), Expect = 1.8e-139
Identity = 280/566 (49.47%), Postives = 358/566 (63.25%), Query Frame = 0


Query: 20  ISLLTFLTFLFLLSSSSFAVVPTAEICQSTTSPSFCKSFLP-NSPASIHSHCRFTLRNAL 79
           ++L   L  LF+ S +      ++ IC++T  P FCKS  P  S   +  + RF+LR +L
Sbjct: 9   VTLFLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFPQTSQGDVREYGRFSLRKSL 68

Query: 80  NHARTFLTLIDAHL---NLLPFPS---ALHDCRGLAEANLDFISQTFSIVNFTTMA--LP 139
             +R F   ID +L   N L   S   AL DCR LA    D++  +F  VN TT +  L 
Sbjct: 69  TQSRKFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETVNITTSSKTLS 128

Query: 140 YYQAHEMMSLISAMITNEDTCYEGL-VGLGSVVGLVDKVLEAISFDRKLYSLYLSLFKMG 199
           + +A E+ +L+SA +TNE TC +G+     S   + + V   +  D KL+S+ L+LF  G
Sbjct: 129 FSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSVSLALFTKG 188

Query: 200 WVSEDMKAPML-----PNMNHFGV------GKGQLKLKMSPKDRAYYERLVHRKKPAAAR 259
           WV +  K         P   H           G L LKM+   RA YE L  R+K A   
Sbjct: 189 WVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESL-SRRKLADDD 248

Query: 260 RLLQTNYEDDGILINGN------NLSDAIAAAPNKSVITKGYFLIYVTAGIYNESVLVPK 319
             + T    D + +N N       +++A+ +APNK+  T GYF+IYVT+G+Y E+V++ K
Sbjct: 249 NDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVVIAK 308

Query: 320 EKRYVLMIGDGNNETIITGNKNVVDGSTTFNSATVAVEGTGFFGVNLTISNTAGAAKHQA 379
            KRY++MIGDG N T++TGN+NVVDG TTFNSAT AV    F  VN+T  NTAG  KHQA
Sbjct: 309 NKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPEKHQA 368

Query: 380 VALRVSADNVTLYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYA 439
           VA+R SAD    YSC FE YQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVV Q+CN+Y 
Sbjct: 369 VAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNLYP 428

Query: 440 RLPLSGQFNALTAQGRTDPNQNTGTSIHNCTIKPTPELAASPAT-KSYLGRPWKEYSRTV 499
           R P+  QFNA+TAQGRTDPNQNTG SIHNCTIKP  +L +S  T K+YLGRPWKEYSRTV
Sbjct: 429 RQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTV 488

Query: 500 YMQSFIGAFIDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRASWV-VGVINATVANN 557
           +MQS+I   ++P GW+EW+G   L+T YYAE+NN+G GS T+ R  W    VIN+T ANN
Sbjct: 489 FMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVINSTDANN 548

[Close]

BLAST of Lsi10G006560 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 485.3 bits (1248), Expect = 6.4e-137
Identity = 275/576 (47.74%), Postives = 356/576 (61.81%), Query Frame = 0


Query: 22  LLTFLTFLFLLSSSSFAVVPTAEICQSTTSPSFCKSFLPNSPASIHSHCRFTLRNALNHA 81
           L+T   FL  + +SS  +  ++ IC++T  P +CKS  P+S  ++  +  F++R +L+ +
Sbjct: 8   LITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFPHSQGNVQQYGCFSIRKSLSQS 67

Query: 82  RTFLTLID------AHLNLLPFPSALHDCRGLAEANLDFISQTFSIVNFTT-----MALP 141
           R F+  +D      AHL+      AL DCR LA   +D++  +F  VN T+       L 
Sbjct: 68  RKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTSFKPLS 127

Query: 142 YYQAHEMMSLISAMITNEDTCYEGLVGLGSVVG---LVDKVLEAISFDRKLYSLYLSLFK 201
           + +A ++ +L+SA +TNE TC EGL    S      +   V   +  D KL  + L+LF 
Sbjct: 128 FPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVSLALFT 187

Query: 202 MGWVSEDMK-------APMLPNMNHFG----VGKGQLKLKMSPKDRAYYERLVHRKKPAA 261
            GWV +  K        P   +  H         G L LKM+ K +A YE L  RK    
Sbjct: 188 KGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAVYESLSRRKLADG 247

Query: 262 ARRLLQTNYEDDGILI--------------NGNNLSDAIAAAPNKSVITKGYFLIYVTAG 321
                     DDG ++              N  N++ A+AAAPN +  + G+FLIYVTAG
Sbjct: 248 D----SNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAG 307

Query: 322 IYNESVLVPKEKRYVLMIGDGNNETIITGNKNVVDGSTTFNSATVAVEGTGFFGVNLTIS 381
           IY E + + K KRY++MIGDG N+T++TGN++VVDG TTFNSAT AV    F  VN+T  
Sbjct: 308 IYEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFR 367

Query: 382 NTAGAAKHQAVALRVSADNVTLYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAA 441
           NTAG  KHQAVALR  AD    YSC FE YQDTLYTHSLRQFYRECD+YGTVDFIFGNAA
Sbjct: 368 NTAGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAA 427

Query: 442 VVLQNCNIYARLPLSGQFNALTAQGRTDPNQNTGTSIHNCTIKPTPELAASPAT-KSYLG 501
           VV QNCN+Y R P+  QFNA+TAQGR+DPNQNTGTSI NCTIKP  +L +S  T K+YLG
Sbjct: 428 VVFQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLVSSNYTVKTYLG 487

Query: 502 RPWKEYSRTVYMQSFIGAFIDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRASWV-V 557
           RPWKEYSRTVYMQS+I  F++P GW+EW+G   L+T YYAE+NN+GPGS+T+ R +W   
Sbjct: 488 RPWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGY 547

[Close]

BLAST of Lsi10G006560 vs. TAIR 10
Match: AT3G10720.2 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 401.4 bits (1030), Expect = 1.2e-111
Identity = 227/547 (41.50%), Postives = 320/547 (58.50%), Query Frame = 0


Query: 46  CQSTTSPSFCKSFL---PNSPASIHSHCRFTLRNALNHARTFLTLIDAHLNLLPFP---- 105
           C+ST  P  C++ L    +SP+  + + +FT++  L  A     +I ++   +       
Sbjct: 83  CKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVITSYARRVESKPGSA 142

Query: 106 -----SALHDCRGLAEANLDFISQTFSIVNFTTMALPYYQAHEMMSLISAMITNEDTCYE 165
                 A+ DC  L+E +++++ +T +    T   +       + SL+S ++TN+ TC +
Sbjct: 143 TAEEIGAVADCGELSELSVNYL-ETVTTELKTAQVMTAALVEHVNSLLSGVVTNQQTCLD 202

Query: 166 GLVGLGSVVGLVDKVLEAISFDRKLYSLYLSLFKMGWVSEDMKAPMLPNMNHFGVGKGQL 225
           GLV   S  G    +   +    +LYS+      +G VS  +      N+  F   KG++
Sbjct: 203 GLVEAKS--GFAAAIGSPMGNLTRLYSI-----SLGLVSHALNR----NLKRFKASKGKI 262

Query: 226 KLKMSPKDRAYYERLVHRKKPA---------AARRLLQTNYEDDGILI------------ 285
               +   R   E L+   +            +R L +      G ++            
Sbjct: 263 LGGGNSTYREPLETLIKGLRKTCDNDKDCRKTSRNLGELGETSGGSILVSKAVIVGPFKS 322

Query: 286 -NGNNLSDAIAAAPNKSVITKGYFLIYVTAGIYNESVLVPKEKRYVLMIGDGNNETIITG 345
            N   ++DAIAAAPN +    GYF+IY   G+Y E ++VP  K+ ++++GDG N+TIITG
Sbjct: 323 DNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTIITG 382

Query: 346 NKNVVDGSTTFNSATVAVEGTGFFGVNLTISNTAGAAKHQAVALRVSADNVTLYSCIFEG 405
           N NVVDG TT+N ++ AV G  F  V++T  NTAG  KHQAVALR +A+  + Y C FEG
Sbjct: 383 NHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCSFEG 442

Query: 406 YQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFNALTAQGRTDP 465
           YQDTLY HSLRQFYRECDIYGTVDFIFGNAA + QNCNIYAR P++ Q NA+TA GR DP
Sbjct: 443 YQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGRLDP 502

Query: 466 NQNTGTSIHNCTIKPTPELAASP-ATKSYLGRPWKEYSRTVYMQSFIGAFIDPAGWKEWD 525
           NQNTG SI NCTIK  P+LAA P +  ++LGRPWK YSRTV+MQS+I   + P GW EW+
Sbjct: 503 NQNTGISIINCTIKAAPDLAAEPKSAMTFLGRPWKPYSRTVFMQSYISDIVQPVGWLEWN 562

Query: 526 GTLNLNTSYYAEFNNSGPGSDTSRRASWV-VGVINATVANNFTVSQFLAGDQWLPPTAVP 557
           GT+ L+T YY E++N GPG++T++R  W+   ++N   A NFTV  F  GD WLP T +P
Sbjct: 563 GTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDTWLPQTDIP 617

[Close]

BLAST of Lsi10G006560 vs. TAIR 10
Match: AT5G04970.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 401.0 bits (1029), Expect = 1.6e-111
Identity = 233/554 (42.06%), Postives = 323/554 (58.30%), Query Frame = 0


Query: 33  SSSSFAVVPTAEICQSTTSPSFCKSFL---PNSPASIHSHCRFTLRNALNHARTFLTLI- 92
           S S  + +P    C+ST  P  C++ L    +SP+  + + +FT++  L  AR    +I 
Sbjct: 75  SPSQPSPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVIN 134

Query: 93  --------DAHLNLLPFPSALHDCRGLAEANLDF---ISQTFSIVNFTTMALPYYQAHEM 152
                   D   + +   SA+ DC  LAE ++++   +++        T AL       +
Sbjct: 135 RFAQRVEADPGTSTVEEVSAVADCGELAELSVEYLETVTEELKAAELMTAAL----VDRV 194

Query: 153 MSLISAMITNEDTCYEGLVGLGSVVGLVDKVLEAISFDRKLYSLYLSLFKMGWVSEDMKA 212
            SL+  ++TN+ TC +GLV   S  G    +   +    +LYS+ L L     ++ ++K 
Sbjct: 195 TSLLGGVVTNQQTCLDGLVDAKS--GFATAIGTPLGNLTRLYSVSLGLVSHA-LNRNLKR 254

Query: 213 PMLPNMNHFGVGKGQLKLKMSPKDRAYYERLVHRKKPAAARRLLQTNYEDDG--ILI--- 272
                   FG G   ++  +    +   +     K    A R L    E  G  IL+   
Sbjct: 255 YKGSKGKIFGGGNKPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGGSILVREA 314

Query: 273 ---------NGNNLSDAIAAAPNKSVITKGYFLIYVTAGIYNESVLVPKEKRYVLMIGDG 332
                    N   +++A+AAAPN +   +GYF+IY  AG+Y E V++  +KR +++IGDG
Sbjct: 315 VTVGPYETDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDG 374

Query: 333 NNETIITGNKNVVDGSTTFNSATVAVEGTGFFGVNLTISNTAGAAKHQAVALRVSADNVT 392
            N+TII+GN + +DG TT+NS+T AV G  F  V++T  NTAG  KHQAVA+R +AD  T
Sbjct: 375 INKTIISGNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGST 434

Query: 393 LYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFNAL 452
            Y C FEGYQDTLY HSLRQFYRECDIYGT+DFIFGNAA + QNCNIYAR P++ Q NA+
Sbjct: 435 FYRCSFEGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAV 494

Query: 453 TAQGRTDPNQNTGTSIHNCTIKPTPELAASP-ATKSYLGRPWKEYSRTVYMQSFIGAFID 512
           TA GRTDPNQ TG SI NCTI   P+LAA P +T ++LGRPWK YSRTVY+QS+I   + 
Sbjct: 495 TAHGRTDPNQKTGISIINCTIGAAPDLAADPKSTMTFLGRPWKPYSRTVYIQSYISDVVQ 554

Query: 513 PAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRASWV-VGVINATVANNFTVSQFLAGDQ 556
           P GW EW+GT  L+T  Y E++N GPG+DTS+R  W    ++N   A NFTV  F  GD 
Sbjct: 555 PVGWLEWNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDT 614

The following BLAST results are available for this feature:

BLAST of Lsi10G006560 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22256	4.4e-143	51.79	Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3...	[more]
Q8RXK7	2.5e-138	49.47	Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3...	[more]
Q9SRX4	9.0e-136	47.74	Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=37...	[more]
Q94CB1	1.7e-110	41.50	Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3...	[more]
Q9FF77	2.2e-110	42.06	Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of Lsi10G006560 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TRW1	1.6e-268	83.99	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold299G00221...	[more]
A0A1S3C644	1.6e-268	83.99	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103497470 PE=3 SV=1	[more]
A0A6J1EJM8	1.8e-248	79.24	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111434929 PE=3 SV=1	[more]
A0A6J1I6B8	1.5e-247	79.06	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111470332 PE=3 SV=1	[more]
A0A6J1L6X0	9.4e-237	75.13	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111499728 PE=3 SV=1	[more]

BLAST of Lsi10G006560 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038902853.1	1.3e-280	87.39	probable pectinesterase/pectinesterase inhibitor 20 [Benincasa hispida]	[more]
XP_008457895.1	3.3e-268	83.99	PREDICTED: pectinesterase-like [Cucumis melo] >KAA0045890.1 pectinesterase-like ...	[more]
XP_004148470.1	1.1e-266	83.60	pectinesterase [Cucumis sativus] >KAE8651957.1 hypothetical protein Csa_006047 [...	[more]
KAG6571391.1	1.2e-249	79.86	putative pectinesterase/pectinesterase inhibitor 41, partial [Cucurbita argyrosp...	[more]
XP_022928019.1	3.8e-248	79.24	pectinesterase-like [Cucurbita moschata]	[more]

BLAST of Lsi10G006560 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G47550.1	3.2e-144	51.79	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G02330.1	1.8e-139	49.47	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT1G02810.1	6.4e-137	47.74	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G10720.2	1.2e-111	41.50	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G04970.1	1.6e-111	42.06	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 36..185 e-value: 0.0027 score: 23.8
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 44..157 e-value: 9.5E-12 score: 45.5
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 256..540 e-value: 6.2E-122 score: 406.0
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 43..188 e-value: 3.3E-13 score: 51.8
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 44..188
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 253..554 e-value: 6.2E-109 score: 366.2
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 26..554
None	No IPR available	PANTHER	PTHR31707:SF275	PECTINESTERASE	coord: 26..554
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 45..190 e-value: 4.30898E-27 score: 105.213
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 390..399
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 256..553

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi10G006560.1	Lsi10G006560.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0030599	pectinesterase activity