Lsi10G005190 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi10G005190
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionprostamide/prostaglandin F synthase
Locationchr10: 7396345 .. 7399857 (+)
RNA-Seq ExpressionLsi10G005190
SyntenyLsi10G005190
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTATTACCAACTCTAATCCATGGGCTACCTTTGTGTTCTCGCCAAATAGTTAATCAAATTGTGACAGTCTATTGCTTCCTCTTCTGGTTGGAAATGACATGAGACTTCTGTGGAGGGAATTTATAGTAAGACAGGGAAATGGAAGGTACTGAACTATACAGCACGTAGAAAAATCAGTTCTCACTTCATCTCTAATCTAAGAAGAAGAAGTGAAGCTAAATTTTGTGGCATACGATACGATTTCATTTCATAGTGAAGCTTCAGAGCTATGAATTCCATTTCTGCTCCCACCCAAAACCCTTTTCCATCCGCTTCTCTTTCGATTTTCCCTTCCTCTTTCTGTTTCAGTTCCAACCTCCCTTCCCAATTTCCCTCTCCTCCATTTCTCAACCCTTATGCTCCCGATTACATCCGCAATTCTCCAAAATTATCAACCCCTAATCCCCATGGTCGTCGCAATTTCAGGCCGCCTTTCGTTTTAAAGGCTTCCACTACTTCAGGGTACGGCCCAGAAATTGGGGAAATTCTCGGCGATGTTCGTATTTTCACTGTTGCTGGTGAGCCAGTTCTGTTCAAGGACCTCTGGGATCAGAGTGAGGTAACGATCATTTCCTCTTCTGGGTTTATCTTTATTTACTGATAGCTGCTCTACGTGAAGTTCTTTTGGTGATTGATTCTGATGAATTGATGATGGATGTGTGTTTATTGCTTAATAAACTGAAAGCTGCAACTGTAGACTGCATATTGCTGAGAAGGGAAAAAGTCAATTAAGAATTGGACATGGAATTCATTGTTATTCTGCATAGTTTTGAATGACCCAAGTCAGAACAAGTTTTAAACATTCTTTATTGGTAATTTATTTGGCTTCCTGAACTATGATATTTCTACCATTCAATCCATTTCTAAGTTTGCCTGTCTTAGTTTGCATTAACTATTACTAACTTAGGCCCATTTTGGTAACCGTTTCGTTTATGAAAATTGAGCGTATTTCCTCTCAATTTCTTATCATGATTTGCATCCTTCTTAAAGTTGAATTGTTTGCCAAATTTCAAAAATAAAAAGTATAACTATGTTTTTTTAGTTTTCAAATTTTGGCTTGGTTTTTAAAAACATTGATAATATGTAGATAACAAAGCAAGAAATTTAGAGGTAGAAAGAATTTTTTTTTATTAAATTTCTTTTATTCCTTTCCCAAGCCACAGGTGTAAGGAGTTTTTATAGACTTAATTTCAAAAACTAAAAATAAGAAACCAAATGGTTACCAAACGGGGTCGTAGCTAACTGAGATCTCAACTGCCAACTGGTAAACAGTGAGTGTAATGTACTTGTCAAGATTTCAAATTCCCCACCCCCAATTTTTCTTGTTAAATAAAGAGACTCGGTTTTTTTCCCTTGCTGACATATTTGACAAACTCCAATGTAATGCTTATCCTGGAATATTTCATGGTAGAATAGATATCCGGAAGTATTTGATTCATGAGTAACTTATCTAGAGAGCTCAATAGTGTTACATTTATTGAATTGATTGTTACAATTTGATACGATATGTAATAAGTTGAGGCATCTATATCTTCATATTAGAAGTTATCGATTCGAATCTCCACTTGCTTTCTTTATTCTTAAAAATGATATTTGATAATATTTGTTTGGTGTTTACCTAGGGAATGGCTGTTGTTGCACTTTTGAGGCATTTTGGATGCTTTTGCTGGTAAGTCTCGTCAATTTGGACCATTCATTTGCAGATTTTTCTTTTTTCCTTTGCAGAAAAAAGGTTAAATGCCCTTTTGTTTTGGTAATTCCATCTGTGTGATTTTGGTGACAGACAGCTACTTGTTCTTATTTCTAGTTGGGAACTTGCTTCTACTTTAAAAGAATCCAAAGCAAGATTTGATTCATCTGGTGTTAAACTAATAGCCGTAGGCATTGGTACACCAAATAAAGCACGTATGCTAGCCGAGCGGGTATCAATTTTTCTTCTGTAAATTAGCACTTAATTGTCCATGGCTGCATCATGTTTTCAAATCCTAAAAGACATTTCATTTTCTAAGATTTACTTATTAACAATAATTCTACTTAGATTAGATGCTCTAAATCAAAATATTATATTTTTGACATCATTGAATTTGGATATTGTTAATATAGTTTAATGTTGATTCTGAAGAGATTCAAACATTTTATAATGTAAGCATTAGTTTGCATTTTAAAAAATTTCTAATAAGTTATTTAACTTTTAAGTTTGTATGTAATAAGTATTTATCGTCAACGTTAACGATAATTTTGAAAGCATTGATTGTAGACCGAACTTATAAATTAAATAAAATGACAAGTATTATCATATCCCTTTTGATTTCTCTGTTTTGCCTTCATGGCATTAACATTGCTTCTTAATCCTTCAAATTCCAGTTACCTTTTCCAATGGATTGCCTTTATGCTGATCCTGATAGGAAGGTAATGCAAAGCTATAAATGTTGATAGCATTATTACATTTATTGTCGTTTTTCTTTGTTTTTTCAAGTTGTGAATATTCGAACCTTCAACCTATAAGATAAGTATGTAATGTCTAAACTAGTTGATGAATGCTGTATATATATATGTAAAAACAATCTGTCAAAGTGAATAATTTGAATTGGCCTTTTAGTTTTTAGTTCTTATAGATGATTGAAGATAAGAGAAGTGAAGACAATTGAAATTTCACATCTTTTTTTATGTTTGTCACTGATTCTTGTGAAAGCTAATCAGAATATGTTCTTGCAGGCATATGATTTACTAGGTCTATACTATGGCTTTGGTCGTACATTCTTTAATCCAGCTAGTGTAAAAATTCTCCTTCCATATACTTTATGATCATTTAAGAGTATTTATTTGCATGTTTTACTTCTTTTTATTTGAACTTTGTTTTCCCCTCGATTGTCAAATGTTTTTAGGTCAAGGTGTTTTCGAAGTCGAGATTTGACGCTACACGAGAAGCCATGAAAAACTATACTTTTGAAGCTACCCCGGACGATAAAAGCAGCGTACTGCAACAGGTCTTGTTTGTGTCTTAATCACACATGAAAAACATTAGCTTTTTGATCTTCCGTTTGTGGAAACTCTCTCTTATTTCAATATGTTTGTGTTGTGATTAGGGAGGGATGTTTGTTTTCAAAGGCAAGCAACTGTTGTATGCTCGAAAAGACGAAGGTACTGGTGACCATGCTCCGTTAGACGACATTTACGACATCTGTTGTCGAGTCCCAGCCTGAAGCAACTGCTGTATGCTCGAAAAGACGGTGACCACACTTCGTTAACAACATCGATGACATCGGTTGTCAAGTCCTAGCCTGAATCTTCACTCATGGCGTGTAAATATTTTGATTGGTATGTATGCTTCTTTTAATCTCTTAGCAAAGCATGAGCAAGGATTAAGATATCTTTGAGGATGACATGTCTGTAAATTGAAAGGTTCAACATTGATATTAATAATTAATCGATCTTGTTGCTGTCTTTTACTAGTCCTAAATTCTTAATATGAA

mRNA sequence

ATTATTACCAACTCTAATCCATGGGCTACCTTTGTGTTCTCGCCAAATAGTTAATCAAATTGTGACAGTCTATTGCTTCCTCTTCTGGTTGGAAATGACATGAGACTTCTGTGGAGGGAATTTATAGTAAGACAGGGAAATGGAAGGTACTGAACTATACAGCACGTAGAAAAATCAGTTCTCACTTCATCTCTAATCTAAGAAGAAGAAGTGAAGCTAAATTTTGTGGCATACGATACGATTTCATTTCATAGTGAAGCTTCAGAGCTATGAATTCCATTTCTGCTCCCACCCAAAACCCTTTTCCATCCGCTTCTCTTTCGATTTTCCCTTCCTCTTTCTGTTTCAGTTCCAACCTCCCTTCCCAATTTCCCTCTCCTCCATTTCTCAACCCTTATGCTCCCGATTACATCCGCAATTCTCCAAAATTATCAACCCCTAATCCCCATGGTCGTCGCAATTTCAGGCCGCCTTTCGTTTTAAAGGCTTCCACTACTTCAGGGTACGGCCCAGAAATTGGGGAAATTCTCGGCGATGTTCGTATTTTCACTGTTGCTGGTGAGCCAGTTCTGTTCAAGGACCTCTGGGATCAGAGTGAGGGAATGGCTGTTGTTGCACTTTTGAGGCATTTTGGATGCTTTTGCTGTTGGGAACTTGCTTCTACTTTAAAAGAATCCAAAGCAAGATTTGATTCATCTGGTGTTAAACTAATAGCCGTAGGCATTGGTACACCAAATAAAGCACGTATGCTAGCCGAGCGGTTACCTTTTCCAATGGATTGCCTTTATGCTGATCCTGATAGGAAGGTCAAGGTGTTTTCGAAGTCGAGATTTGACGCTACACGAGAAGCCATGAAAAACTATACTTTTGAAGCTACCCCGGACGATAAAAGCAGCGTACTGCAACAGGGAGGGATGTTTGTTTTCAAAGGCAAGCAACTGTTGTATGCTCGAAAAGACGAAGGTACTGGTGACCATGCTCCGTTAGACGACATTTACGACATCTGTTGTCGAGTCCCAGCCTGAAGCAACTGCTGTATGCTCGAAAAGACGGTGACCACACTTCGTTAACAACATCGATGACATCGGTTGTCAAGTCCTAGCCTGAATCTTCACTCATGGCGTGTAAATATTTTGATTGGTATGTATGCTTCTTTTAATCTCTTAGCAAAGCATGAGCAAGGATTAAGATATCTTTGAGGATGACATGTCTGTAAATTGAAAGGTTCAACATTGATATTAATAATTAATCGATCTTGTTGCTGTCTTTTACTAGTCCTAAATTCTTAATATGAA

Coding sequence (CDS)

ATGAATTCCATTTCTGCTCCCACCCAAAACCCTTTTCCATCCGCTTCTCTTTCGATTTTCCCTTCCTCTTTCTGTTTCAGTTCCAACCTCCCTTCCCAATTTCCCTCTCCTCCATTTCTCAACCCTTATGCTCCCGATTACATCCGCAATTCTCCAAAATTATCAACCCCTAATCCCCATGGTCGTCGCAATTTCAGGCCGCCTTTCGTTTTAAAGGCTTCCACTACTTCAGGGTACGGCCCAGAAATTGGGGAAATTCTCGGCGATGTTCGTATTTTCACTGTTGCTGGTGAGCCAGTTCTGTTCAAGGACCTCTGGGATCAGAGTGAGGGAATGGCTGTTGTTGCACTTTTGAGGCATTTTGGATGCTTTTGCTGTTGGGAACTTGCTTCTACTTTAAAAGAATCCAAAGCAAGATTTGATTCATCTGGTGTTAAACTAATAGCCGTAGGCATTGGTACACCAAATAAAGCACGTATGCTAGCCGAGCGGTTACCTTTTCCAATGGATTGCCTTTATGCTGATCCTGATAGGAAGGTCAAGGTGTTTTCGAAGTCGAGATTTGACGCTACACGAGAAGCCATGAAAAACTATACTTTTGAAGCTACCCCGGACGATAAAAGCAGCGTACTGCAACAGGGAGGGATGTTTGTTTTCAAAGGCAAGCAACTGTTGTATGCTCGAAAAGACGAAGGTACTGGTGACCATGCTCCGTTAGACGACATTTACGACATCTGTTGTCGAGTCCCAGCCTGA

Protein sequence

MNSISAPTQNPFPSASLSIFPSSFCFSSNLPSQFPSPPFLNPYAPDYIRNSPKLSTPNPHGRRNFRPPFVLKASTTSGYGPEIGEILGDVRIFTVAGEPVLFKDLWDQSEGMAVVALLRHFGCFCCWELASTLKESKARFDSSGVKLIAVGIGTPNKARMLAERLPFPMDCLYADPDRKVKVFSKSRFDATREAMKNYTFEATPDDKSSVLQQGGMFVFKGKQLLYARKDEGTGDHAPLDDIYDICCRVPA
Homology
[Close]
BLAST of Lsi10G005190 vs. ExPASy Swiss-Prot
Match: Q58CY6 (Prostamide/prostaglandin F synthase OS=Bos taurus OX=9913 GN=PRXL2B PE=2 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 2.0e-05
Identity = 51/165 (30.91%), Postives = 71/165 (43.03%), Query Frame = 0 

Query: 95  VAGEPVLFKDLWDQSEGMAVVALLRHFGCFCCWELASTLKESKARFDSSGVKLIAVGIGT 154
           V GE V  ++LW   E   VVA LR FGC  C  +A  L   K   D  GV+L+ VG   
Sbjct: 18  VTGEAVELRNLW--QEQACVVAGLRRFGCMVCRWIARDLSNLKGLLDQHGVRLVGVG--- 77

Query: 155 PNKARMLAERLP--FPMDCLYADPDRK-VKVFSKSRFDA-----------TRE-AMKNYT 214
             +A  L E L   +    LY D  ++  K     R+++            RE A K   
Sbjct: 78  -PEALGLQEFLDGGYFAGELYLDESKQFYKELGFKRYNSLSILPAALGKPVREVAAKAKA 137

Query: 215 FEATPDDKSSVLQQGGMFVFK--GKQLLYARKDEGTGDHAPLDDI 243
                +    +LQ GG+ V    G ++L     +  GD+APL+ I
Sbjct: 138 VGIQGNLSGDLLQSGGLLVVAKGGDKVLLHFVQKSPGDYAPLESI 176

[Close]
BLAST of Lsi10G005190 vs. ExPASy Swiss-Prot
Match: A9CQL8 (Prostamide/prostaglandin F synthase OS=Sus scrofa OX=9823 GN=PRXL2B PE=1 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 4.4e-05
Identity = 51/171 (29.82%), Postives = 72/171 (42.11%), Query Frame = 0 

Query: 95  VAGEPVLFKDLWDQSEGMAVVALLRHFGCFCCWELASTLKESKARFDSSGVKLIAVGIGT 154
           V GE V  + LW   E   VVA LR FGC  C  +A  L   K   D  GV+L+ VG   
Sbjct: 18  VTGEAVELRSLW--QEQACVVAGLRRFGCMVCRWIARDLSSLKGLLDQHGVRLVGVG--- 77

Query: 155 PNKARMLAERLP---FPMDCLYADPDRK-VKVFSKSRFD-------ATREAMKNYTFEAT 214
             +A  L E L    F  D LY D  ++  K     R+        A  + +++   +A 
Sbjct: 78  -PEALGLQEFLDGGYFAGD-LYLDESKQFYKELGFKRYSSLSILPAALGKPVRDVAAKAK 137

Query: 215 P-----DDKSSVLQQGGMFVFK--GKQLLYARKDEGTGDHAPLDDIYDICC 248
                 +    +LQ GG+ V    G ++L     +  GD+AP + I    C
Sbjct: 138 AAGIQGNLSGDLLQSGGLLVVAKGGDKVLLHFVQKSPGDYAPQESILQALC 181

[Close]
BLAST of Lsi10G005190 vs. ExPASy TrEMBL
Match: A0A1S4E1M8 (prostamide/prostaglandin F synthase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103497363 PE=4 SV=1)

HSP 1 Score: 421.4 bits (1082), Expect = 2.7e-114
Identity = 213/271 (78.60%), Postives = 225/271 (83.03%), Query Frame = 0 

Query: 1   MNSISAPTQNPFPSASLSIFPSSFCFSSNLPSQFPSPPFLNPYAPDYIRNSPKLSTPNPH 60
           MNSIS  T  PF S+SLSIFPSSF F  N PSQFPS PFLNP +P+ I NS +L TPNPH
Sbjct: 1   MNSISTHTHYPFRSSSLSIFPSSFTF--NFPSQFPSHPFLNPISPNCILNSARLRTPNPH 60

Query: 61  GRRNFRPPFVLKASTTSGYGPEIGEILGDVRIFTVAGEPVLFKDLWDQSEGMAVVALLRH 120
            RRN+R   VLKAST+SGY PEIGEILGDVRIFT  GEPVLFKDLWDQ+EGMAVVALLRH
Sbjct: 61  VRRNYRSSQVLKASTSSGYAPEIGEILGDVRIFTAGGEPVLFKDLWDQTEGMAVVALLRH 120

Query: 121 FGCFCCWELASTLKESKARFDSSGVKLIAVGIGTPNKARMLAERLPFPMDCLYADPDRK- 180
           FGCFCCWELASTLKE+K RFDSSGVKLIAVGIGTPNKAR+LAERLPFPMDCLYADPDRK 
Sbjct: 121 FGCFCCWELASTLKEAKERFDSSGVKLIAVGIGTPNKARILAERLPFPMDCLYADPDRKA 180

Query: 181 -------------------VKVFSKSRFDATREAMKNYTFEATPDDKSSVLQQGGMFVFK 240
                              VKVFSK+RFD  REAMKNYT EATPDDKSSVLQQGGMFVFK
Sbjct: 181 YDLLGLYYGFGRTFLNPASVKVFSKTRFDVMREAMKNYTLEATPDDKSSVLQQGGMFVFK 240

Query: 241 GKQLLYARKDEGTGDHAPLDDIYDICCRVPA 252
           GKQLLYARKDEGTGDHAPLDDIY+ICC+VPA
Sbjct: 241 GKQLLYARKDEGTGDHAPLDDIYNICCQVPA 269

[Close]
BLAST of Lsi10G005190 vs. ExPASy TrEMBL
Match: A0A5D3BK60 (Prostamide/prostaglandin F synthase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G00840 PE=4 SV=1)

HSP 1 Score: 421.4 bits (1082), Expect = 2.7e-114
Identity = 213/271 (78.60%), Postives = 225/271 (83.03%), Query Frame = 0 

Query: 1   MNSISAPTQNPFPSASLSIFPSSFCFSSNLPSQFPSPPFLNPYAPDYIRNSPKLSTPNPH 60
           MNSIS  T  PF S+SLSIFPSSF F  N PSQFPS PFLNP +P+ I NS +L TPNPH
Sbjct: 1   MNSISTHTHYPFRSSSLSIFPSSFTF--NFPSQFPSHPFLNPISPNCILNSARLRTPNPH 60

Query: 61  GRRNFRPPFVLKASTTSGYGPEIGEILGDVRIFTVAGEPVLFKDLWDQSEGMAVVALLRH 120
            RRN+R   VLKAST+SGY PEIGEILGDVRIFT  GEPVLFKDLWDQ+EGMAVVALLRH
Sbjct: 61  VRRNYRSSQVLKASTSSGYAPEIGEILGDVRIFTAGGEPVLFKDLWDQTEGMAVVALLRH 120

Query: 121 FGCFCCWELASTLKESKARFDSSGVKLIAVGIGTPNKARMLAERLPFPMDCLYADPDRK- 180
           FGCFCCWELASTLKE+K RFDSSGVKLIAVGIGTPNKAR+LAERLPFPMDCLYADPDRK 
Sbjct: 121 FGCFCCWELASTLKEAKERFDSSGVKLIAVGIGTPNKARILAERLPFPMDCLYADPDRKA 180

Query: 181 -------------------VKVFSKSRFDATREAMKNYTFEATPDDKSSVLQQGGMFVFK 240
                              VKVFSK+RFD  REAMKNYT EATPDDKSSVLQQGGMFVFK
Sbjct: 181 YDLLGLYYGFGRTFLNPASVKVFSKTRFDVMREAMKNYTLEATPDDKSSVLQQGGMFVFK 240

Query: 241 GKQLLYARKDEGTGDHAPLDDIYDICCRVPA 252
           GKQLLYARKDEGTGDHAPLDDIY+ICC+VPA
Sbjct: 241 GKQLLYARKDEGTGDHAPLDDIYNICCQVPA 269

[Close]
BLAST of Lsi10G005190 vs. ExPASy TrEMBL
Match: A0A0A0LJF1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G279270 PE=4 SV=1)

HSP 1 Score: 417.2 bits (1071), Expect = 5.0e-113
Identity = 216/272 (79.41%), Postives = 224/272 (82.35%), Query Frame = 0 

Query: 1   MNSISAPTQNPFPSASLSIFPSSFCFSSNLPSQFPSPPFLNPYAPDYIRNSPKLSTPNPH 60
           MNSISA T NPF SAS SIFPSSF F  N PSQFPS PFLNP +P  I NS +L T NPH
Sbjct: 1   MNSISAHTHNPFRSASHSIFPSSFTF--NFPSQFPSHPFLNPISPSCILNSARLRTSNPH 60

Query: 61  GRRNFRPPFVLKASTTSGYGPEIGEILGDVRIFTVAGEPVLFKDLWDQSEGMAVVALLRH 120
            RRNFR   VLKAST+SGY PEIGEILGDVRIFT  GEPVLFKDLWDQ+EGMAVVALLRH
Sbjct: 61  VRRNFRSSQVLKASTSSGYVPEIGEILGDVRIFTAGGEPVLFKDLWDQTEGMAVVALLRH 120

Query: 121 FGCFCCWELASTLKESKARFDSSGVKLIAVGIGTPNKARMLAERLPFPMDCLYADPDRK- 180
           FGCFCCWELASTLKESK RFDSSGVKLIAVGIGTPNKAR+LAERLPFPMDCLYADPDRK 
Sbjct: 121 FGCFCCWELASTLKESKERFDSSGVKLIAVGIGTPNKARILAERLPFPMDCLYADPDRKA 180

Query: 181 -------------------VKVFSKSRFDATREAMKNYTFEATPDDKSSVLQQGGMFVFK 240
                              VKVFSK+R D  REAMKNYTFEATPDDKSSVLQQGGMFVFK
Sbjct: 181 YDLLGLYYGFGRTFLNPASVKVFSKTRLDIMREAMKNYTFEATPDDKSSVLQQGGMFVFK 240

Query: 241 GKQLLYARKDEGTGDHAPLDDIYDICC-RVPA 252
           GKQLLYARKDEGTGDHAPLDDIY+ICC +VPA
Sbjct: 241 GKQLLYARKDEGTGDHAPLDDIYNICCTQVPA 270

[Close]
BLAST of Lsi10G005190 vs. ExPASy TrEMBL
Match: A0A6J1I579 (prostamide/prostaglandin F synthase OS=Cucurbita maxima OX=3661 GN=LOC111469330 PE=4 SV=1)

HSP 1 Score: 404.1 bits (1037), Expect = 4.4e-109
Identity = 207/269 (76.95%), Postives = 216/269 (80.30%), Query Frame = 0 

Query: 1   MNSISAPTQNPFPSASLSIFPSSFCFSSNLPSQFPSPPFLNPYAPDYIRNSPKLSTPNPH 60
           MN +SA TQNP   ASLSI PSSF    NLPS FPSP  L PYAPD    S KL+T N  
Sbjct: 1   MNLVSARTQNPLRCASLSILPSSF----NLPSHFPSPLLLKPYAPDSKLYSAKLATTNGD 60

Query: 61  GRRNFRPPFVLKASTTSGYGPEIGEILGDVRIFTVAGEPVLFKDLWDQSEGMAVVALLRH 120
           GRRNFR P V+KAS TSGYGPEIGE LGDVRIFT AGEPVLFKDLWDQ+EG+AVVALLRH
Sbjct: 61  GRRNFRSPVVVKASITSGYGPEIGENLGDVRIFTAAGEPVLFKDLWDQTEGIAVVALLRH 120

Query: 121 FGCFCCWELASTLKESKARFDSSGVKLIAVGIGTPNKARMLAERLPFPMDCLYADPDRK- 180
           FGCFCCWELASTLKESKARFDSSGVKLIAVGIGTPNKAR+LAERLPFPMDCLYADPDRK 
Sbjct: 121 FGCFCCWELASTLKESKARFDSSGVKLIAVGIGTPNKARILAERLPFPMDCLYADPDRKA 180

Query: 181 -------------------VKVFSKSRFDATREAMKNYTFEATPDDKSSVLQQGGMFVFK 240
                               KVFSKSRF   REAMKNYT EATPDD+SSVLQQGGM VFK
Sbjct: 181 YDLLGLYYGFGRTFFNPASTKVFSKSRFTTLREAMKNYTLEATPDDRSSVLQQGGMLVFK 240

Query: 241 GKQLLYARKDEGTGDHAPLDDIYDICCRV 250
           GKQLLYARKDEGTGDHAPLD+IYD+CCRV
Sbjct: 241 GKQLLYARKDEGTGDHAPLDEIYDVCCRV 265

[Close]
BLAST of Lsi10G005190 vs. ExPASy TrEMBL
Match: A0A6J1ETX1 (prostamide/prostaglandin F synthase OS=Cucurbita moschata OX=3662 GN=LOC111436511 PE=4 SV=1)

HSP 1 Score: 400.6 bits (1028), Expect = 4.8e-108
Identity = 205/270 (75.93%), Postives = 214/270 (79.26%), Query Frame = 0 

Query: 1   MNSISAPTQNPFPSASLSIFPSSFCFSSNLPSQFPSPPFLNPYAPDYIRNSPKLSTPNPH 60
           MN +SA TQNP   ASLS+  SS     NLPS FPSP  L PYAPD      KL+T N  
Sbjct: 1   MNLVSARTQNPLRCASLSMLSSSI----NLPSHFPSPLLLKPYAPDSKLYPAKLATTNGD 60

Query: 61  GRRNFRPPFVLKASTTSGYGPEIGEILGDVRIFTVAGEPVLFKDLWDQSEGMAVVALLRH 120
            RRNFR P V+KAS TSGYGPEIGEILGDVRIFT AGEPVLFKDLWDQ+EGMAVVALLRH
Sbjct: 61  ARRNFRSPVVVKASITSGYGPEIGEILGDVRIFTAAGEPVLFKDLWDQTEGMAVVALLRH 120

Query: 121 FGCFCCWELASTLKESKARFDSSGVKLIAVGIGTPNKARMLAERLPFPMDCLYADPDRK- 180
           FGCFCCWELASTLKESKARFDSSGVKLIAVGIGTPNKAR+LAERLPFPMDCLYADPDRK 
Sbjct: 121 FGCFCCWELASTLKESKARFDSSGVKLIAVGIGTPNKARILAERLPFPMDCLYADPDRKA 180

Query: 181 -------------------VKVFSKSRFDATREAMKNYTFEATPDDKSSVLQQGGMFVFK 240
                               KVFSKSRF   REAMKNYT EATPDD+SSVLQQGGM VFK
Sbjct: 181 YDLLGLYYGFGRTFFNPASTKVFSKSRFSTLREAMKNYTLEATPDDRSSVLQQGGMLVFK 240

Query: 241 GKQLLYARKDEGTGDHAPLDDIYDICCRVP 251
           GKQLLYARKDEGTGDHAPLD+IYD+CCRVP
Sbjct: 241 GKQLLYARKDEGTGDHAPLDEIYDVCCRVP 266

[Close]
BLAST of Lsi10G005190 vs. NCBI nr
Match: XP_008457741.1 (PREDICTED: prostamide/prostaglandin F synthase isoform X1 [Cucumis melo] >XP_016902130.1 PREDICTED: prostamide/prostaglandin F synthase isoform X1 [Cucumis melo] >XP_016902131.1 PREDICTED: prostamide/prostaglandin F synthase isoform X1 [Cucumis melo] >KAA0045765.1 prostamide/prostaglandin F synthase isoform X1 [Cucumis melo var. makuwa] >TYJ99517.1 prostamide/prostaglandin F synthase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 421.4 bits (1082), Expect = 5.5e-114
Identity = 213/271 (78.60%), Postives = 225/271 (83.03%), Query Frame = 0 

Query: 1   MNSISAPTQNPFPSASLSIFPSSFCFSSNLPSQFPSPPFLNPYAPDYIRNSPKLSTPNPH 60
           MNSIS  T  PF S+SLSIFPSSF F  N PSQFPS PFLNP +P+ I NS +L TPNPH
Sbjct: 1   MNSISTHTHYPFRSSSLSIFPSSFTF--NFPSQFPSHPFLNPISPNCILNSARLRTPNPH 60

Query: 61  GRRNFRPPFVLKASTTSGYGPEIGEILGDVRIFTVAGEPVLFKDLWDQSEGMAVVALLRH 120
            RRN+R   VLKAST+SGY PEIGEILGDVRIFT  GEPVLFKDLWDQ+EGMAVVALLRH
Sbjct: 61  VRRNYRSSQVLKASTSSGYAPEIGEILGDVRIFTAGGEPVLFKDLWDQTEGMAVVALLRH 120

Query: 121 FGCFCCWELASTLKESKARFDSSGVKLIAVGIGTPNKARMLAERLPFPMDCLYADPDRK- 180
           FGCFCCWELASTLKE+K RFDSSGVKLIAVGIGTPNKAR+LAERLPFPMDCLYADPDRK 
Sbjct: 121 FGCFCCWELASTLKEAKERFDSSGVKLIAVGIGTPNKARILAERLPFPMDCLYADPDRKA 180

Query: 181 -------------------VKVFSKSRFDATREAMKNYTFEATPDDKSSVLQQGGMFVFK 240
                              VKVFSK+RFD  REAMKNYT EATPDDKSSVLQQGGMFVFK
Sbjct: 181 YDLLGLYYGFGRTFLNPASVKVFSKTRFDVMREAMKNYTLEATPDDKSSVLQQGGMFVFK 240

Query: 241 GKQLLYARKDEGTGDHAPLDDIYDICCRVPA 252
           GKQLLYARKDEGTGDHAPLDDIY+ICC+VPA
Sbjct: 241 GKQLLYARKDEGTGDHAPLDDIYNICCQVPA 269

[Close]
BLAST of Lsi10G005190 vs. NCBI nr
Match: XP_004149685.1 (prostamide/prostaglandin F synthase isoform X1 [Cucumis sativus] >KGN61990.1 hypothetical protein Csa_006427 [Cucumis sativus])

HSP 1 Score: 417.2 bits (1071), Expect = 1.0e-112
Identity = 216/272 (79.41%), Postives = 224/272 (82.35%), Query Frame = 0 

Query: 1   MNSISAPTQNPFPSASLSIFPSSFCFSSNLPSQFPSPPFLNPYAPDYIRNSPKLSTPNPH 60
           MNSISA T NPF SAS SIFPSSF F  N PSQFPS PFLNP +P  I NS +L T NPH
Sbjct: 1   MNSISAHTHNPFRSASHSIFPSSFTF--NFPSQFPSHPFLNPISPSCILNSARLRTSNPH 60

Query: 61  GRRNFRPPFVLKASTTSGYGPEIGEILGDVRIFTVAGEPVLFKDLWDQSEGMAVVALLRH 120
            RRNFR   VLKAST+SGY PEIGEILGDVRIFT  GEPVLFKDLWDQ+EGMAVVALLRH
Sbjct: 61  VRRNFRSSQVLKASTSSGYVPEIGEILGDVRIFTAGGEPVLFKDLWDQTEGMAVVALLRH 120

Query: 121 FGCFCCWELASTLKESKARFDSSGVKLIAVGIGTPNKARMLAERLPFPMDCLYADPDRK- 180
           FGCFCCWELASTLKESK RFDSSGVKLIAVGIGTPNKAR+LAERLPFPMDCLYADPDRK 
Sbjct: 121 FGCFCCWELASTLKESKERFDSSGVKLIAVGIGTPNKARILAERLPFPMDCLYADPDRKA 180

Query: 181 -------------------VKVFSKSRFDATREAMKNYTFEATPDDKSSVLQQGGMFVFK 240
                              VKVFSK+R D  REAMKNYTFEATPDDKSSVLQQGGMFVFK
Sbjct: 181 YDLLGLYYGFGRTFLNPASVKVFSKTRLDIMREAMKNYTFEATPDDKSSVLQQGGMFVFK 240

Query: 241 GKQLLYARKDEGTGDHAPLDDIYDICC-RVPA 252
           GKQLLYARKDEGTGDHAPLDDIY+ICC +VPA
Sbjct: 241 GKQLLYARKDEGTGDHAPLDDIYNICCTQVPA 270

[Close]
BLAST of Lsi10G005190 vs. NCBI nr
Match: XP_038902955.1 (prostamide/prostaglandin F synthase [Benincasa hispida])

HSP 1 Score: 410.6 bits (1054), Expect = 9.7e-111
Identity = 212/271 (78.23%), Postives = 223/271 (82.29%), Query Frame = 0 

Query: 1   MNSISAPTQNPFPSASLSIFPSSFCFSSNLPSQFPSPPFLNPYAPDYIRNSPKLSTPNPH 60
           MNSISA TQNPF SAS SIFP++F F  N PSQFP      P++PD    S KLSTPNPH
Sbjct: 1   MNSISARTQNPFRSASFSIFPATFTF--NPPSQFPF-----PHSPDSTLYSAKLSTPNPH 60

Query: 61  GRRNFRPPFVLKASTTSGYGPEIGEILGDVRIFTVAGEPVLFKDLWDQSEGMAVVALLRH 120
           GRRNFR P VLKASTT+ Y PEIGEILGDVRIFT AG+PVLFKDLWDQSEGMAVVALLRH
Sbjct: 61  GRRNFRSPLVLKASTTA-YAPEIGEILGDVRIFTAAGDPVLFKDLWDQSEGMAVVALLRH 120

Query: 121 FGCFCCWELASTLKESKARFDSSGVKLIAVGIGTPNKARMLAERLPFPMDCLYADPDRK- 180
           FGCFCCWE ASTLKESKARFDSSGVKLIAVGIGTPNKAR+LAERLPFPMDCLYADPDRK 
Sbjct: 121 FGCFCCWEFASTLKESKARFDSSGVKLIAVGIGTPNKARILAERLPFPMDCLYADPDRKA 180

Query: 181 -------------------VKVFSKSRFDATREAMKNYTFEATPDDKSSVLQQGGMFVFK 240
                               KVFSKSR +A REAMKNY+ EATPDDKSSVLQQGGMFVFK
Sbjct: 181 YDLLGLYYGFGRTFFNPASAKVFSKSRLEAMREAMKNYSLEATPDDKSSVLQQGGMFVFK 240

Query: 241 GKQLLYARKDEGTGDHAPLDDIYDICCRVPA 252
           GKQLLYARKDEGTGDHAPL+DIY+ICCRVPA
Sbjct: 241 GKQLLYARKDEGTGDHAPLEDIYNICCRVPA 263

[Close]
BLAST of Lsi10G005190 vs. NCBI nr
Match: XP_022970334.1 (prostamide/prostaglandin F synthase [Cucurbita maxima])

HSP 1 Score: 404.1 bits (1037), Expect = 9.1e-109
Identity = 207/269 (76.95%), Postives = 216/269 (80.30%), Query Frame = 0 

Query: 1   MNSISAPTQNPFPSASLSIFPSSFCFSSNLPSQFPSPPFLNPYAPDYIRNSPKLSTPNPH 60
           MN +SA TQNP   ASLSI PSSF    NLPS FPSP  L PYAPD    S KL+T N  
Sbjct: 1   MNLVSARTQNPLRCASLSILPSSF----NLPSHFPSPLLLKPYAPDSKLYSAKLATTNGD 60

Query: 61  GRRNFRPPFVLKASTTSGYGPEIGEILGDVRIFTVAGEPVLFKDLWDQSEGMAVVALLRH 120
           GRRNFR P V+KAS TSGYGPEIGE LGDVRIFT AGEPVLFKDLWDQ+EG+AVVALLRH
Sbjct: 61  GRRNFRSPVVVKASITSGYGPEIGENLGDVRIFTAAGEPVLFKDLWDQTEGIAVVALLRH 120

Query: 121 FGCFCCWELASTLKESKARFDSSGVKLIAVGIGTPNKARMLAERLPFPMDCLYADPDRK- 180
           FGCFCCWELASTLKESKARFDSSGVKLIAVGIGTPNKAR+LAERLPFPMDCLYADPDRK 
Sbjct: 121 FGCFCCWELASTLKESKARFDSSGVKLIAVGIGTPNKARILAERLPFPMDCLYADPDRKA 180

Query: 181 -------------------VKVFSKSRFDATREAMKNYTFEATPDDKSSVLQQGGMFVFK 240
                               KVFSKSRF   REAMKNYT EATPDD+SSVLQQGGM VFK
Sbjct: 181 YDLLGLYYGFGRTFFNPASTKVFSKSRFTTLREAMKNYTLEATPDDRSSVLQQGGMLVFK 240

Query: 241 GKQLLYARKDEGTGDHAPLDDIYDICCRV 250
           GKQLLYARKDEGTGDHAPLD+IYD+CCRV
Sbjct: 241 GKQLLYARKDEGTGDHAPLDEIYDVCCRV 265

[Close]
BLAST of Lsi10G005190 vs. NCBI nr
Match: XP_023519132.1 (prostamide/prostaglandin F synthase [Cucurbita pepo subsp. pepo] >XP_023519133.1 prostamide/prostaglandin F synthase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 403.3 bits (1035), Expect = 1.5e-108
Identity = 207/270 (76.67%), Postives = 214/270 (79.26%), Query Frame = 0 

Query: 1   MNSISAPTQNPFPSASLSIFPSSFCFSSNLPSQFPSPPFLNPYAPDYIRNSPKLSTPNPH 60
           MN +SA TQNP   ASLSI  SS     NLPS FPSP  L PYAPD    S KL+T N  
Sbjct: 1   MNLVSASTQNPLRCASLSILSSSI----NLPSHFPSPLLLKPYAPDSKLYSAKLATTNGD 60

Query: 61  GRRNFRPPFVLKASTTSGYGPEIGEILGDVRIFTVAGEPVLFKDLWDQSEGMAVVALLRH 120
            RRNFR P V KAS TSGYGPEIGEILGDVRIFT AGEPVLFKDLWDQ+EGMAVVALLRH
Sbjct: 61  ARRNFRSPVVAKASITSGYGPEIGEILGDVRIFTAAGEPVLFKDLWDQTEGMAVVALLRH 120

Query: 121 FGCFCCWELASTLKESKARFDSSGVKLIAVGIGTPNKARMLAERLPFPMDCLYADPDRK- 180
           FGCFCCWELASTLKESKARFDSSGVKLIAVGIGTPNKAR+LAERLPFPMDCLYADPDRK 
Sbjct: 121 FGCFCCWELASTLKESKARFDSSGVKLIAVGIGTPNKARILAERLPFPMDCLYADPDRKA 180

Query: 181 -------------------VKVFSKSRFDATREAMKNYTFEATPDDKSSVLQQGGMFVFK 240
                               KVFSKSRF   REAMKNYT EATPDD+SSVLQQGGM VFK
Sbjct: 181 YDLLGLYYGFGRTFFNPASTKVFSKSRFSTLREAMKNYTLEATPDDRSSVLQQGGMLVFK 240

Query: 241 GKQLLYARKDEGTGDHAPLDDIYDICCRVP 251
           GKQLLYARKDEGTGDHAPLD+IYD+CCRVP
Sbjct: 241 GKQLLYARKDEGTGDHAPLDEIYDVCCRVP 266

[Close]
BLAST of Lsi10G005190 vs. TAIR 10
Match: AT5G65840.1 (Thioredoxin superfamily protein )

HSP 1 Score: 255.8 bits (652), Expect = 3.7e-68
Identity = 125/206 (60.68%), Postives = 149/206 (72.33%), Query Frame = 0 

Query: 66  RPPFVLKASTT---SGYGPEIGEILGDVRIFTVAGEPVLFKDLWDQSEGMAVVALLRHFG 125
           RP  V   + T   + Y  +IGEILGDV IFT +G+ V F DLWDQ +G+A V LLRHFG
Sbjct: 68  RPRVVSARAATESFTDYREDIGEILGDVSIFTASGQRVQFSDLWDQKDGIAAVVLLRHFG 127

Query: 126 CFCCWELASTLKESKARFDSSGVKLIAVGIGTPNKARMLAERLPFPMDCLYADPDRK--- 185
           C CCWELA+ LKE+K RFD++GVKLIAVG+GTP+KAR+LA RLPFPM+CLYADP+RK   
Sbjct: 128 CVCCWELATALKEAKPRFDAAGVKLIAVGVGTPDKARILATRLPFPMECLYADPERKAYD 187

Query: 186 -----------------VKVFSKSRFDATREAMKNYTFEATPDDKSSVLQQGGMFVFKGK 245
                             KVF  SRF   REA KNYT EATP+D+SSVLQQGG FVF+GK
Sbjct: 188 VLGLYFGLGRTFFNPASTKVF--SRFSEIREATKNYTIEATPEDRSSVLQQGGTFVFRGK 247

Query: 246 QLLYARKDEGTGDHAPLDDIYDICCR 249
           +LLY RKDEGTGDH  LDD+ ++CC+
Sbjct: 248 KLLYGRKDEGTGDHPSLDDVINVCCK 271

The following BLAST results are available for this feature:
BLAST of Lsi10G005190 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q58CY62.0e-0530.91Prostamide/prostaglandin F synthase OS=Bos taurus OX=9913 GN=PRXL2B PE=2 SV=1[more]
A9CQL84.4e-0529.82Prostamide/prostaglandin F synthase OS=Sus scrofa OX=9823 GN=PRXL2B PE=1 SV=1[more]
BLAST of Lsi10G005190 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A1S4E1M82.7e-11478.60prostamide/prostaglandin F synthase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A5D3BK602.7e-11478.60Prostamide/prostaglandin F synthase isoform X1 OS=Cucumis melo var. makuwa OX=11... [more]
A0A0A0LJF15.0e-11379.41Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G279270 PE=4 SV=1[more]
A0A6J1I5794.4e-10976.95prostamide/prostaglandin F synthase OS=Cucurbita maxima OX=3661 GN=LOC111469330 ... [more]
A0A6J1ETX14.8e-10875.93prostamide/prostaglandin F synthase OS=Cucurbita moschata OX=3662 GN=LOC11143651... [more]
BLAST of Lsi10G005190 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_008457741.15.5e-11478.60PREDICTED: prostamide/prostaglandin F synthase isoform X1 [Cucumis melo] >XP_016... [more]
XP_004149685.11.0e-11279.41prostamide/prostaglandin F synthase isoform X1 [Cucumis sativus] >KGN61990.1 hyp... [more]
XP_038902955.19.7e-11178.23prostamide/prostaglandin F synthase [Benincasa hispida][more]
XP_022970334.19.1e-10976.95prostamide/prostaglandin F synthase [Cucurbita maxima][more]
XP_023519132.11.5e-10876.67prostamide/prostaglandin F synthase [Cucurbita pepo subsp. pepo] >XP_023519133.1... [more]
BLAST of Lsi10G005190 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G65840.13.7e-6860.68Thioredoxin superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.30.10Glutaredoxincoord: 80..246
e-value: 3.5E-7
score: 31.9
NoneNo IPR availablePANTHERPTHR28630:SF26OS03G0729300 PROTEINcoord: 181..250
coord: 76..179
NoneNo IPR availableCDDcd02970PRX_like2coord: 87..231
e-value: 1.47821E-30
score: 109.371
IPR032801Peroxiredoxin-like 2A/B/CPFAMPF13911AhpC-TSA_2coord: 133..232
e-value: 1.1E-11
score: 44.8
IPR032801Peroxiredoxin-like 2A/B/CPANTHERPTHR28630FAMILY NOT NAMEDcoord: 181..250
coord: 76..179
IPR036249Thioredoxin-like superfamilySUPERFAMILY52833Thioredoxin-likecoord: 82..185

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi10G005190.1Lsi10G005190.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0098869 cellular oxidant detoxification
molecular_function GO:0016209 antioxidant activity