Lsi10G005170 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi10G005170
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionGTP-binding protein At2g22870
Locationchr10: 7379208 .. 7381465 (+)
RNA-Seq ExpressionLsi10G005170
SyntenyLsi10G005170
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTACTGAAGAGCCTCAGCCGCCTCCAAAACCTTCCTCTCGCCTCTCTCTTTTCCCCAACTCTCTCTCCAAAATCCTCTCCCCACTTTCTCTCTTTCTCAGCCCCGCTTTCACATTCCACCCCTCCGAATCGTTCCCCATCCCACCTTGTTTCCGCTTCCAACGCCACCGCCAAGACATCAATCTACGATTCCGCTCGATTCGCCCGCACGGTTCTGTTTGTTCCTCCTGGAGTCGACGCCGATGAGGTTTCCGAAGAAATGATCCTTCCAGGCTCCAATATTGTGCTCGGCCCTTACGCCGGCGACTCTCGTGTCAAGGAGGTTGAGTTCGTCAAAAGCAGCGCTCGGGCTAGGGATTGCCCTAAAGATAACAAACCTGAATTTGCGATTCTTGGTCGTTCCAATGTCGGCAAATCTTCACTTATTAATGCCCTTGTTCGCAAAAAGGAAGTTGCTCTTACTTCCAAGAAGCCAGGTCTGTTTGATTTTACGTTTGTTTTAACTATTCCGTATGCCACTGGAATCTATTTGTGGTTAATACCATTGAATTTCTGTTTGATTGCAGGGAAGACGCAGCTGATTAATCACTTCTTAGTGAATAAAAGCTGGTACATAGTGGACTTACCAGGCTATGGGTAATTTCTGGTTCTTTTCTTGCAAGCTATATTTGGGTTGTACTCTTTTACATAAGTCTAACGATTGAGATGTTTATGTGCATATTTGTGCAATTACTTTTCGTTTTTGCATGTTGGGGCTGTCCTTTTTGGTGATATTTTGTTTTGGAGATTCTATGTTAACTCATATGATTTGATTAGTACAGTGGAGGAAATAAAGTACTGTTTTTCTTTTTAGTTATGTAGTTCAATGGCTGCTATCTGTTCTTTGTCTCTTAATAATACACTATTGCTTTTCCAAATTGGTGTGCTTGGTCTTTTAAGTCGATAGCTACTTCTCTAGCTTCTATTGCTGTTTGGATGATCGGATTGGTCATATGGGTTGATCTTGTTTATAATTTCTTTAGTAATAGGTAAACTGGGGGATTGTCCAACTACTATGTAAACCTGTTTAAGTGATCAATTACTACCAATTTGATGCATTAGAGGCTTATAGTAAGTGTTTTGTTTTATTTTATTTTATTTTTATGGTAGTGGTGCTATTTTGCTATATATTTCTGTTTTTTTTTGCAAGATATGGAGCTTTCAGGGATCTTAGGGAGTGTTTCTAAGTGTTCTAAAATTATAAAAGCACTTCTAGTAAGCAGGCAAATATCGTGAAACGTATTTTTTAACCAGCAGAATTTCTCTTAAGAATTCTAAAAATTGAATTGAACTCTAGAATCAAAACCGTGAAGATTTTGAGAACCAATTCATAAAAGCTTAAGATGGCACGGTAAATTATCTATCATCCTTCTCAACTATCTCAACAGAATTTGATGTGGAATGGAACCAAAAATGTAAAAGATTTGGTTGTGTATTGCTTTGCCATTTTGTTTATAAGGTGCCTTTTCTTCGTTCTCTAATTTGGTCCCTCAAGAATTTCCCCTTGGCTTGAGATATTTTGTTCTATTAAGAGTTTTATTGTTAATAAGAGGTTTCAACTTTCGACCAAAAAGAGGTCCGAAGAGCATTGCTATCTTCTTAAGGTAGATAAAGTTTTTAAGTTGTATTAACTTCTTTTACTCTAAATGCAAAGCAATCACTACTTCTACTTCTGCTTCTACGTATTGTTTTGTAGTTGTTTAACACTTCAAATTTGATTCAGTTTTGCCAAAGCCTCGGAAACTGCTCGAATGGATTGGTCCTCATTCACAAAAGGGTATTTTATAAACCGAGATACTTTAGTAGCTGTGCTGCTTCTTGTTGATGCAAGTGTTCCACCTCAAAAAATAGACTTAGATTGCGCCAATTGGCTTGGCCGCAACAATGTATGCTATCTGAATTTAAGTTCATTGCTTTTTATGTTGAAATATGGTGCTAGTTGAATACATAGATAGTTCTTGTCTGATATAATTACCAAATATCTTATGTTGTGTTGTGCGTTTTCAGATACCGATGACCTTTGTTTTCACGAAATGCGACAAAATGAAGGTGAGCAAAGGAAAAAGACCCGACGAGAACATCAGGGAGTTTCAACAACTTATCAGACAGAATTACAAGGTACATCCTTCTTGGATCATGACTAGTAGTGTCACAGGTTTAGGTCGGGATGAATTACTTCTACACATGTCGCAACTGAGGAATTACTGGGATCAATAA

mRNA sequence

ATGTTACTGAAGAGCCTCAGCCGCCTCCAAAACCTTCCTCTCGCCTCTCTCTTTTCCCCAACTCTCTCTCCAAAATCCTCTCCCCACTTTCTCTCTTTCTCAGCCCCGCTTTCACATTCCACCCCTCCGAATCGTTCCCCATCCCACCTTGTTTCCGCTTCCAACGCCACCGCCAAGACATCAATCTACGATTCCGCTCGATTCGCCCGCACGGTTCTGTTTGTTCCTCCTGGAGTCGACGCCGATGAGGTTTCCGAAGAAATGATCCTTCCAGGCTCCAATATTGTGCTCGGCCCTTACGCCGGCGACTCTCGTGTCAAGGAGGTTGAGTTCGTCAAAAGCAGCGCTCGGGCTAGGGATTGCCCTAAAGATAACAAACCTGAATTTGCGATTCTTGGTCGTTCCAATGTCGGCAAATCTTCACTTATTAATGCCCTTGTTCGCAAAAAGGAAGTTGCTCTTACTTCCAAGAAGCCAGGTCTGTTTGATTTTACGTTTGTTTTAACTATTCCGTATGCCACTGGAATCTATTTGTGGTTAATACCATTGAATTTCTGTTTGATTGCAGGGAAGACGCAGCTGATTAATCACTTCTTAGTGAATAAAAGCTGGTACATAGTGGACTTACCAGGCTATGGTTTTGCCAAAGCCTCGGAAACTGCTCGAATGGATTGGTCCTCATTCACAAAAGGGTATTTTATAAACCGAGATACTTTAGTAGCTGTGCTGCTTCTTGTTGATGCAAGTGTTCCACCTCAAAAAATAGACTTAGATTGCGCCAATTGGCTTGGCCGCAACAATATACCGATGACCTTTGTTTTCACGAAATGCGACAAAATGAAGGTGAGCAAAGGAAAAAGACCCGACGAGAACATCAGGGAGTTTCAACAACTTATCAGACAGAATTACAAGGTACATCCTTCTTGGATCATGACTAGTAGTGTCACAGGTTTAGGTCGGGATGAATTACTTCTACACATGTCGCAACTGAGGAATTACTGGGATCAATAA

Coding sequence (CDS)

ATGTTACTGAAGAGCCTCAGCCGCCTCCAAAACCTTCCTCTCGCCTCTCTCTTTTCCCCAACTCTCTCTCCAAAATCCTCTCCCCACTTTCTCTCTTTCTCAGCCCCGCTTTCACATTCCACCCCTCCGAATCGTTCCCCATCCCACCTTGTTTCCGCTTCCAACGCCACCGCCAAGACATCAATCTACGATTCCGCTCGATTCGCCCGCACGGTTCTGTTTGTTCCTCCTGGAGTCGACGCCGATGAGGTTTCCGAAGAAATGATCCTTCCAGGCTCCAATATTGTGCTCGGCCCTTACGCCGGCGACTCTCGTGTCAAGGAGGTTGAGTTCGTCAAAAGCAGCGCTCGGGCTAGGGATTGCCCTAAAGATAACAAACCTGAATTTGCGATTCTTGGTCGTTCCAATGTCGGCAAATCTTCACTTATTAATGCCCTTGTTCGCAAAAAGGAAGTTGCTCTTACTTCCAAGAAGCCAGGTCTGTTTGATTTTACGTTTGTTTTAACTATTCCGTATGCCACTGGAATCTATTTGTGGTTAATACCATTGAATTTCTGTTTGATTGCAGGGAAGACGCAGCTGATTAATCACTTCTTAGTGAATAAAAGCTGGTACATAGTGGACTTACCAGGCTATGGTTTTGCCAAAGCCTCGGAAACTGCTCGAATGGATTGGTCCTCATTCACAAAAGGGTATTTTATAAACCGAGATACTTTAGTAGCTGTGCTGCTTCTTGTTGATGCAAGTGTTCCACCTCAAAAAATAGACTTAGATTGCGCCAATTGGCTTGGCCGCAACAATATACCGATGACCTTTGTTTTCACGAAATGCGACAAAATGAAGGTGAGCAAAGGAAAAAGACCCGACGAGAACATCAGGGAGTTTCAACAACTTATCAGACAGAATTACAAGGTACATCCTTCTTGGATCATGACTAGTAGTGTCACAGGTTTAGGTCGGGATGAATTACTTCTACACATGTCGCAACTGAGGAATTACTGGGATCAATAA

Protein sequence

MLLKSLSRLQNLPLASLFSPTLSPKSSPHFLSFSAPLSHSTPPNRSPSHLVSASNATAKTSIYDSARFARTVLFVPPGVDADEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARARDCPKDNKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGLFDFTFVLTIPYATGIYLWLIPLNFCLIAGKTQLINHFLVNKSWYIVDLPGYGFAKASETARMDWSSFTKGYFINRDTLVAVLLLVDASVPPQKIDLDCANWLGRNNIPMTFVFTKCDKMKVSKGKRPDENIREFQQLIRQNYKVHPSWIMTSSVTGLGRDELLLHMSQLRNYWDQ
Homology
BLAST of Lsi10G005170 vs. ExPASy Swiss-Prot
Match: O81004 (GTP-binding protein At2g22870 OS=Arabidopsis thaliana OX=3702 GN=EMB2001 PE=2 SV=2)

HSP 1 Score: 426.8 bits (1096), Expect = 2.3e-118
Identity = 211/318 (66.35%), Postives = 247/318 (77.67%), Query Frame = 0

Query: 21  TLSPKSSPHFLSFSAPLSHSTPPNRSPSHLVSASNATAKT--SIYDSARFARTVLFVPPG 80
           TL    +P+F+S              P    S +N T  T  SI D+ +FA++VLF+PPG
Sbjct: 26  TLQSFRNPNFISI-------------PKISASTNNPTTTTNRSISDATKFAKSVLFIPPG 85

Query: 81  VDADEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARARDCPKDNKPEFAILGRSNVG 140
           V+ +E++++M+LPGSNIV+GP+AG S++KEVEFVKSSARARDCPKD++PE AILGRSNVG
Sbjct: 86  VEIEELTDDMVLPGSNIVIGPFAGHSQIKEVEFVKSSARARDCPKDDRPEIAILGRSNVG 145

Query: 141 KSSLINALVRKKEVALTSKKPGLFDFTFVLTIPYATGIYLWLIPLNFCLIAGKTQLINHF 200
           KSSLIN LVRKKEVALTSKKP                              GKTQLINHF
Sbjct: 146 KSSLINCLVRKKEVALTSKKP------------------------------GKTQLINHF 205

Query: 201 LVNKSWYIVDLPGYGFAKASETARMDWSSFTKGYFINRDTLVAVLLLVDASVPPQKIDLD 260
           LVNKSWYIVDLPGYGFAK S+ A+ DWS+FTKGYF+NRD+LV VLLL+DASVPPQKIDLD
Sbjct: 206 LVNKSWYIVDLPGYGFAKVSDAAKTDWSAFTKGYFLNRDSLVCVLLLIDASVPPQKIDLD 265

Query: 261 CANWLGRNNIPMTFVFTKCDKMKVSKGKRPDENIREFQQLIRQNYKVHPSWIMTSSVTGL 320
           CANWLGRNN+PMTFVFTKCDKMK +KGKRPDENI+ FQQ+IR+N+KVHP WI+TSSV+GL
Sbjct: 266 CANWLGRNNVPMTFVFTKCDKMKATKGKRPDENIKAFQQIIRENFKVHPPWILTSSVSGL 300

Query: 321 GRDELLLHMSQLRNYWDQ 337
           GRDELLLHMSQLRNYWDQ
Sbjct: 326 GRDELLLHMSQLRNYWDQ 300

BLAST of Lsi10G005170 vs. ExPASy Swiss-Prot
Match: A0M537 (Probable GTP-binding protein EngB OS=Gramella forsetii (strain KT0803) OX=411154 GN=engB PE=3 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 1.3e-41
Identity = 84/228 (36.84%), Postives = 131/228 (57.46%), Query Frame = 0

Query: 105 RVKEVEFVKSSARARDCPKDNKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGLFDF 164
           ++K  EFV S+++   CP    PE+A +GRSNVGKSSLIN L  +K +A TS KP     
Sbjct: 2   KIKTAEFVISNSKVDHCPNSTLPEYAFIGRSNVGKSSLINMLTGRKSLAKTSAKP----- 61

Query: 165 TFVLTIPYATGIYLWLIPLNFCLIAGKTQLINHFLVNKSWYIVDLPGYGFAKASETARMD 224
                                    GKTQLINHFL+N +W++VDLPGYG+A+ S++ +  
Sbjct: 62  -------------------------GKTQLINHFLINNNWHLVDLPGYGYAQVSKSTKKV 121

Query: 225 WSSFTKGYFINRDTLVAVLLLVDASVPPQKIDLDCANWLGRNNIPMTFVFTKCDKMKVSK 284
           +  F   YF  R+ ++   +L+D+   PQ ID++   WLG +NIP   +FTK DK+   K
Sbjct: 122 FQKFITAYFKKREQMICAFVLIDSRHKPQPIDMEFMQWLGEHNIPFCIIFTKADKL---K 181

Query: 285 GKRPDENIREFQQLIRQNYKVHPSWIMTSSVTGLGRDELLLHMSQLRN 333
            K  D+NI  ++  + +++   P + +TS+ + LG+D++L ++  + N
Sbjct: 182 PKILDKNIENYKNEMLESWVEMPEYFITSATSKLGQDDILDYIEGINN 196

BLAST of Lsi10G005170 vs. ExPASy Swiss-Prot
Match: A5FIZ1 (Probable GTP-binding protein EngB OS=Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / JCM 8514 / NBRC 14942 / NCIMB 11054 / UW101) OX=376686 GN=engB PE=3 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 1.5e-40
Identity = 86/226 (38.05%), Postives = 125/226 (55.31%), Query Frame = 0

Query: 105 RVKEVEFVKSSARARDCPKDNKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGLFDF 164
           ++   EF+ S++ A  CPKD  PE+A +GRSNVGKSSLIN +   K +A TS KP     
Sbjct: 2   KINTAEFIVSNSDAAKCPKDFLPEYAFIGRSNVGKSSLINMITNHKNLAKTSGKP----- 61

Query: 165 TFVLTIPYATGIYLWLIPLNFCLIAGKTQLINHFLVNKSWYIVDLPGYGFAKASETARMD 224
                                    GKTQLINHF +N +W++VDLPGYG+AK S+  +  
Sbjct: 62  -------------------------GKTQLINHFKINNNWFLVDLPGYGYAKVSKKTKSI 121

Query: 225 WSSFTKGYFINRDTLVAVLLLVDASVPPQKIDLDCANWLGRNNIPMTFVFTKCDKMKVSK 284
           +  F   YF NR+ LV   +L+D     QKID++  +++G + IP   +FTK D  K+SK
Sbjct: 122 FQQFITDYFENREQLVCAFVLIDIRHEAQKIDIEFMSYMGESEIPFCIIFTKAD--KISK 181

Query: 285 GKRPDENIREFQQLIRQNYKVHPSWIMTSSVTGLGRDELLLHMSQL 331
           GK         +Q+   N+   P + +TSS   +G+DELL ++ ++
Sbjct: 182 GKIDSHIAAYRKQMYANNWAEMPQYFVTSSTESIGKDELLTYIDEV 195

BLAST of Lsi10G005170 vs. ExPASy Swiss-Prot
Match: A6LBY4 (Probable GTP-binding protein EngB OS=Parabacteroides distasonis (strain ATCC 8503 / DSM 20701 / CIP 104284 / JCM 5825 / NCTC 11152) OX=435591 GN=engB PE=3 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 3.3e-40
Identity = 88/227 (38.77%), Postives = 126/227 (55.51%), Query Frame = 0

Query: 106 VKEVEFVKSSARARDCPKDNKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGLFDFT 165
           +K  EFV S+   R CP+ NKPE+A +GRSNVGKSSLIN L  KK +A+TS+ P      
Sbjct: 3   IKSAEFVISNTDVRKCPEGNKPEYAFIGRSNVGKSSLINMLTNKKGLAMTSQTP------ 62

Query: 166 FVLTIPYATGIYLWLIPLNFCLIAGKTQLINHFLVNKSWYIVDLPGYGFAKASETARMDW 225
                                   GKT LINHFL+N  WY+VDLPGYGFA+  +  R   
Sbjct: 63  ------------------------GKTLLINHFLINNEWYLVDLPGYGFAQRGKENREQL 122

Query: 226 SSFTKGYFINRDTLVAVLLLVDASVPPQKIDLDCANWLGRNNIPMTFVFTKCDKMKVSKG 285
               + Y ++R+ L  + +L+D     QKIDL+   WLG + +P + +FTK D  K+SKG
Sbjct: 123 KRIIENYILDREQLTNLFVLLDCRHEAQKIDLEFMEWLGESGVPFSIIFTKID--KISKG 182

Query: 286 KRPDENIREFQQLIRQNYKVHPSWIMTSSVTGLGRDELLLHMSQLRN 333
            R  EN++ +   + + ++  P   ++SS    GRDE+L ++  + N
Sbjct: 183 -RLKENLKVYTDKLLETWEELPPIFLSSSEKKEGRDEILDYIESINN 196

BLAST of Lsi10G005170 vs. ExPASy Swiss-Prot
Match: Q5LDL2 (Probable GTP-binding protein EngB OS=Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343) OX=272559 GN=engB PE=3 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 4.7e-39
Identity = 84/225 (37.33%), Postives = 123/225 (54.67%), Query Frame = 0

Query: 106 VKEVEFVKSSARARDCPKDNKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGLFDFT 165
           +   EFV S+   + CP    PE+A +GRSNVGKSSLIN L  +K +A+TS  P      
Sbjct: 3   ITNAEFVISNTDVKKCPAGTFPEYAFIGRSNVGKSSLINMLTGRKGLAMTSATP------ 62

Query: 166 FVLTIPYATGIYLWLIPLNFCLIAGKTQLINHFLVNKSWYIVDLPGYGFAKASETARMDW 225
                                   GKT LINHFL+N SWY+VDLPGYG+A+  +  +   
Sbjct: 63  ------------------------GKTMLINHFLINNSWYLVDLPGYGYARRGQKGQKQI 122

Query: 226 SSFTKGYFINRDTLVAVLLLVDASVPPQKIDLDCANWLGRNNIPMTFVFTKCDKMKVSKG 285
            +  + Y + R+ +  + +L+D+ + PQKIDL+   WLG N IP   +FTK DK+   KG
Sbjct: 123 RTIIEDYILEREQMTNLFVLIDSRLEPQKIDLEFMEWLGENGIPFAIIFTKADKL---KG 182

Query: 286 KRPDENIREFQQLIRQNYKVHPSWIMTSSVTGLGRDELLLHMSQL 331
            R   NI  + + +R+ ++  P + +TSS   LGR E+L ++  +
Sbjct: 183 GRLKINISAYLRELRKQWEELPPYFITSSEERLGRTEVLNYIESI 194

BLAST of Lsi10G005170 vs. ExPASy TrEMBL
Match: A0A6J1J701 (GTP-binding protein At2g22870 OS=Cucurbita maxima OX=3661 GN=LOC111484051 PE=3 SV=1)

HSP 1 Score: 566.2 bits (1458), Expect = 8.9e-158
Identity = 291/336 (86.61%), Postives = 296/336 (88.10%), Query Frame = 0

Query: 1   MLLKSLSRLQNLPLASLFSPTLSPKSSPHFLSFSAPLSHSTPPNRSPSHLVSASNATAKT 60
           M+LK+LSRL  LPLASLFSPTLSPKSSPHFL  S+PLSHST PNRSPS L S SN TAKT
Sbjct: 1   MILKTLSRLHTLPLASLFSPTLSPKSSPHFLPISSPLSHSTAPNRSPSLLASVSNTTAKT 60

Query: 61  SIYDSARFARTVLFVPPGVDADEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARARD 120
           SI DSARFARTVLFVPPGVDADEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARARD
Sbjct: 61  SISDSARFARTVLFVPPGVDADEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARARD 120

Query: 121 CPKDNKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGLFDFTFVLTIPYATGIYLWL 180
           CPKDNKPEFAILGRSNVGKSSLINALVRKKEVALTSKKP                     
Sbjct: 121 CPKDNKPEFAILGRSNVGKSSLINALVRKKEVALTSKKP--------------------- 180

Query: 181 IPLNFCLIAGKTQLINHFLVNKSWYIVDLPGYGFAKASETARMDWSSFTKGYFINRDTLV 240
                    GKTQLINHFLVNKSWYIVDLPGYGFAKASETARMDWSSFTKGYFINRDTLV
Sbjct: 181 ---------GKTQLINHFLVNKSWYIVDLPGYGFAKASETARMDWSSFTKGYFINRDTLV 240

Query: 241 AVLLLVDASVPPQKIDLDCANWLGRNNIPMTFVFTKCDKMKVSKGKRPDENIREFQQLIR 300
           AVLLLVDASVPPQKIDLDCANWLGRNNIP+TFVFTKCDKMKVSKGKRPDENIR+FQQLIR
Sbjct: 241 AVLLLVDASVPPQKIDLDCANWLGRNNIPLTFVFTKCDKMKVSKGKRPDENIRDFQQLIR 300

Query: 301 QNYKVHPSWIMTSSVTGLGRDELLLHMSQLRNYWDQ 337
           QNYKVHPSWIMTSSVTGLGRDELLLHMSQLRNYWDQ
Sbjct: 301 QNYKVHPSWIMTSSVTGLGRDELLLHMSQLRNYWDQ 306

BLAST of Lsi10G005170 vs. ExPASy TrEMBL
Match: A0A6J1EIY5 (GTP-binding protein At2g22870 OS=Cucurbita moschata OX=3662 GN=LOC111434809 PE=3 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 5.8e-157
Identity = 290/336 (86.31%), Postives = 295/336 (87.80%), Query Frame = 0

Query: 1   MLLKSLSRLQNLPLASLFSPTLSPKSSPHFLSFSAPLSHSTPPNRSPSHLVSASNATAKT 60
           M+LK+LSRL  LPLASLFSPTLSPKSSPHFL  S+PLSHST PNRSPS L S SN TAKT
Sbjct: 1   MILKTLSRLHTLPLASLFSPTLSPKSSPHFLPVSSPLSHSTAPNRSPSLLASVSNTTAKT 60

Query: 61  SIYDSARFARTVLFVPPGVDADEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARARD 120
           SI DSARFARTVLFVPPGVDADEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARARD
Sbjct: 61  SISDSARFARTVLFVPPGVDADEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARARD 120

Query: 121 CPKDNKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGLFDFTFVLTIPYATGIYLWL 180
           CPKDNKPEFAILGRSNVGKSSLINALVRKKEVALTSKKP                     
Sbjct: 121 CPKDNKPEFAILGRSNVGKSSLINALVRKKEVALTSKKP--------------------- 180

Query: 181 IPLNFCLIAGKTQLINHFLVNKSWYIVDLPGYGFAKASETARMDWSSFTKGYFINRDTLV 240
                    GKTQLINHFLVNKSWYIVDLPGYGFAKASETAR DWSSFTKGYFINRDTLV
Sbjct: 181 ---------GKTQLINHFLVNKSWYIVDLPGYGFAKASETARTDWSSFTKGYFINRDTLV 240

Query: 241 AVLLLVDASVPPQKIDLDCANWLGRNNIPMTFVFTKCDKMKVSKGKRPDENIREFQQLIR 300
           AVLLLVDASVPPQKIDLDCANWLGRNNIP+TFVFTKCDKMKVSKGKRPDENIR+FQQLIR
Sbjct: 241 AVLLLVDASVPPQKIDLDCANWLGRNNIPLTFVFTKCDKMKVSKGKRPDENIRDFQQLIR 300

Query: 301 QNYKVHPSWIMTSSVTGLGRDELLLHMSQLRNYWDQ 337
           QNYKVHPSWIMTSSVTGLGRDELLLHMSQLRNYWDQ
Sbjct: 301 QNYKVHPSWIMTSSVTGLGRDELLLHMSQLRNYWDQ 306

BLAST of Lsi10G005170 vs. ExPASy TrEMBL
Match: A0A5D3BI84 (GTP-binding protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G00870 PE=3 SV=1)

HSP 1 Score: 530.0 bits (1364), Expect = 7.1e-147
Identity = 274/336 (81.55%), Postives = 283/336 (84.23%), Query Frame = 0

Query: 1   MLLKSLSRLQNLPLASLFSPTLSPKSSPHFLSFSAPLSHSTPPNRSPSHLVSASNATAKT 60
           MLLK+ SRL   PLASLFSPTLSPK SPH LSFS             S LVS+SNATAK+
Sbjct: 1   MLLKNFSRLHTFPLASLFSPTLSPKPSPHSLSFS-------------SRLVSSSNATAKS 60

Query: 61  SIYDSARFARTVLFVPPGVDADEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARARD 120
           SI DSARFARTVLF+PPGVDAD++S+E ILPGSNIVLGPYAGDSRVKEVEFVKSS RARD
Sbjct: 61  SISDSARFARTVLFIPPGVDADDISDEKILPGSNIVLGPYAGDSRVKEVEFVKSSGRARD 120

Query: 121 CPKDNKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGLFDFTFVLTIPYATGIYLWL 180
           CPKDNKPEFAILGRSNVGKSSLINALVRKKEVALTSKKP                     
Sbjct: 121 CPKDNKPEFAILGRSNVGKSSLINALVRKKEVALTSKKP--------------------- 180

Query: 181 IPLNFCLIAGKTQLINHFLVNKSWYIVDLPGYGFAKASETARMDWSSFTKGYFINRDTLV 240
                    GKTQLINHFLVNKSWYIVDLPGYGFAKASETARMDWSSFTKGYF+NRDTLV
Sbjct: 181 ---------GKTQLINHFLVNKSWYIVDLPGYGFAKASETARMDWSSFTKGYFMNRDTLV 240

Query: 241 AVLLLVDASVPPQKIDLDCANWLGRNNIPMTFVFTKCDKMKVSKGKRPDENIREFQQLIR 300
           AVLLL+DASVPPQKIDLDCANWLGRNNIPMTFVFTKCDKMKVSKGKRPDENIREFQQLIR
Sbjct: 241 AVLLLIDASVPPQKIDLDCANWLGRNNIPMTFVFTKCDKMKVSKGKRPDENIREFQQLIR 293

Query: 301 QNYKVHPSWIMTSSVTGLGRDELLLHMSQLRNYWDQ 337
           QNYKVHPSWIMTSSVTGLGRDELLLHMSQLRNYWDQ
Sbjct: 301 QNYKVHPSWIMTSSVTGLGRDELLLHMSQLRNYWDQ 293

BLAST of Lsi10G005170 vs. ExPASy TrEMBL
Match: A0A1S3C6C3 (GTP-binding protein At2g22870 OS=Cucumis melo OX=3656 GN=LOC103497360 PE=3 SV=1)

HSP 1 Score: 530.0 bits (1364), Expect = 7.1e-147
Identity = 274/336 (81.55%), Postives = 283/336 (84.23%), Query Frame = 0

Query: 1   MLLKSLSRLQNLPLASLFSPTLSPKSSPHFLSFSAPLSHSTPPNRSPSHLVSASNATAKT 60
           MLLK+ SRL   PLASLFSPTLSPK SPH LSFS             S LVS+SNATAK+
Sbjct: 1   MLLKNFSRLHTFPLASLFSPTLSPKPSPHSLSFS-------------SRLVSSSNATAKS 60

Query: 61  SIYDSARFARTVLFVPPGVDADEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARARD 120
           SI DSARFARTVLF+PPGVDAD++S+E ILPGSNIVLGPYAGDSRVKEVEFVKSS RARD
Sbjct: 61  SISDSARFARTVLFIPPGVDADDISDEKILPGSNIVLGPYAGDSRVKEVEFVKSSGRARD 120

Query: 121 CPKDNKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGLFDFTFVLTIPYATGIYLWL 180
           CPKDNKPEFAILGRSNVGKSSLINALVRKKEVALTSKKP                     
Sbjct: 121 CPKDNKPEFAILGRSNVGKSSLINALVRKKEVALTSKKP--------------------- 180

Query: 181 IPLNFCLIAGKTQLINHFLVNKSWYIVDLPGYGFAKASETARMDWSSFTKGYFINRDTLV 240
                    GKTQLINHFLVNKSWYIVDLPGYGFAKASETARMDWSSFTKGYF+NRDTLV
Sbjct: 181 ---------GKTQLINHFLVNKSWYIVDLPGYGFAKASETARMDWSSFTKGYFMNRDTLV 240

Query: 241 AVLLLVDASVPPQKIDLDCANWLGRNNIPMTFVFTKCDKMKVSKGKRPDENIREFQQLIR 300
           AVLLL+DASVPPQKIDLDCANWLGRNNIPMTFVFTKCDKMKVSKGKRPDENIREFQQLIR
Sbjct: 241 AVLLLIDASVPPQKIDLDCANWLGRNNIPMTFVFTKCDKMKVSKGKRPDENIREFQQLIR 293

Query: 301 QNYKVHPSWIMTSSVTGLGRDELLLHMSQLRNYWDQ 337
           QNYKVHPSWIMTSSVTGLGRDELLLHMSQLRNYWDQ
Sbjct: 301 QNYKVHPSWIMTSSVTGLGRDELLLHMSQLRNYWDQ 293

BLAST of Lsi10G005170 vs. ExPASy TrEMBL
Match: A0A6J1D8D5 (GTP-binding protein At2g22870 OS=Momordica charantia OX=3673 GN=LOC111017869 PE=3 SV=1)

HSP 1 Score: 516.9 bits (1330), Expect = 6.2e-143
Identity = 270/338 (79.88%), Postives = 284/338 (84.02%), Query Frame = 0

Query: 1   MLLKSLSRLQNLPLASLFSPTLSPKSSPH--FLSFSAPLSHSTPPNRSPSHLVSASNATA 60
           M+LK  SRLQNLPLA++FS  LSPK+SP    LSF  PLSHS  P+  P  L  +++  A
Sbjct: 1   MILK--SRLQNLPLAAVFSAALSPKTSPGPLSLSFRTPLSHSAAPHPPPEGLKRSASNAA 60

Query: 61  KTSIYDSARFARTVLFVPPGVDADEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARA 120
           K SI DSARFARTVLF+PPGV+ DEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARA
Sbjct: 61  KPSISDSARFARTVLFIPPGVEPDEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARA 120

Query: 121 RDCPKDNKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGLFDFTFVLTIPYATGIYL 180
           RDCPKD+KPEFAILGRSNVGKSSLINALVRKKEVALTSKKP                   
Sbjct: 121 RDCPKDDKPEFAILGRSNVGKSSLINALVRKKEVALTSKKP------------------- 180

Query: 181 WLIPLNFCLIAGKTQLINHFLVNKSWYIVDLPGYGFAKASETARMDWSSFTKGYFINRDT 240
                      GKTQLINHFLVNKSWYIVDLPGYGFAKASE ARMDWSSFTKGYFINRDT
Sbjct: 181 -----------GKTQLINHFLVNKSWYIVDLPGYGFAKASEAARMDWSSFTKGYFINRDT 240

Query: 241 LVAVLLLVDASVPPQKIDLDCANWLGRNNIPMTFVFTKCDKMKVSKGKRPDENIREFQQL 300
           LVAVLLL+DASVPPQKIDLDCANWLGRNNIPMTFVFTKCDKMKVSKGKRPDENIR+FQQL
Sbjct: 241 LVAVLLLIDASVPPQKIDLDCANWLGRNNIPMTFVFTKCDKMKVSKGKRPDENIRDFQQL 300

Query: 301 IRQNYKVHPSWIMTSSVTGLGRDELLLHMSQLRNYWDQ 337
           IRQNYKVHPSWIMTSSVTGLGRDELLLHMSQLRN+WDQ
Sbjct: 301 IRQNYKVHPSWIMTSSVTGLGRDELLLHMSQLRNFWDQ 306

BLAST of Lsi10G005170 vs. NCBI nr
Match: XP_038900534.1 (GTP-binding protein At2g22870 [Benincasa hispida])

HSP 1 Score: 575.1 bits (1481), Expect = 4.0e-160
Identity = 296/336 (88.10%), Postives = 300/336 (89.29%), Query Frame = 0

Query: 1   MLLKSLSRLQNLPLASLFSPTLSPKSSPHFLSFSAPLSHSTPPNRSPSHLVSASNATAKT 60
           MLLKSLSRL NLPLASLFSPTLSPKSSPHFLSFSA LSHST PNRSP HL+SASNATAKT
Sbjct: 1   MLLKSLSRLHNLPLASLFSPTLSPKSSPHFLSFSARLSHSTTPNRSPPHLISASNATAKT 60

Query: 61  SIYDSARFARTVLFVPPGVDADEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARARD 120
           SI DSARFARTVLFVPPGVDAD+VSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARARD
Sbjct: 61  SISDSARFARTVLFVPPGVDADDVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARARD 120

Query: 121 CPKDNKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGLFDFTFVLTIPYATGIYLWL 180
           CPKDNKPEFAILGRSNVGKSSLINALVRKKEVALTSKKP                     
Sbjct: 121 CPKDNKPEFAILGRSNVGKSSLINALVRKKEVALTSKKP--------------------- 180

Query: 181 IPLNFCLIAGKTQLINHFLVNKSWYIVDLPGYGFAKASETARMDWSSFTKGYFINRDTLV 240
                    G+TQLINHFLVNKSWYIVDLPGYGFAKASETARMDWSSFTKGYFINRDTLV
Sbjct: 181 ---------GRTQLINHFLVNKSWYIVDLPGYGFAKASETARMDWSSFTKGYFINRDTLV 240

Query: 241 AVLLLVDASVPPQKIDLDCANWLGRNNIPMTFVFTKCDKMKVSKGKRPDENIREFQQLIR 300
           AVLLLVDASVPPQKIDLDCANWLGRNNIPMTFVFTKCDKMKVSKGKRPDENI+EF QLIR
Sbjct: 241 AVLLLVDASVPPQKIDLDCANWLGRNNIPMTFVFTKCDKMKVSKGKRPDENIKEFLQLIR 300

Query: 301 QNYKVHPSWIMTSSVTGLGRDELLLHMSQLRNYWDQ 337
           QNYKVHPSWIMTSSVTGLGRDELLLHMSQLRNYWDQ
Sbjct: 301 QNYKVHPSWIMTSSVTGLGRDELLLHMSQLRNYWDQ 306

BLAST of Lsi10G005170 vs. NCBI nr
Match: XP_022986232.1 (GTP-binding protein At2g22870 [Cucurbita maxima])

HSP 1 Score: 566.2 bits (1458), Expect = 1.8e-157
Identity = 291/336 (86.61%), Postives = 296/336 (88.10%), Query Frame = 0

Query: 1   MLLKSLSRLQNLPLASLFSPTLSPKSSPHFLSFSAPLSHSTPPNRSPSHLVSASNATAKT 60
           M+LK+LSRL  LPLASLFSPTLSPKSSPHFL  S+PLSHST PNRSPS L S SN TAKT
Sbjct: 1   MILKTLSRLHTLPLASLFSPTLSPKSSPHFLPISSPLSHSTAPNRSPSLLASVSNTTAKT 60

Query: 61  SIYDSARFARTVLFVPPGVDADEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARARD 120
           SI DSARFARTVLFVPPGVDADEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARARD
Sbjct: 61  SISDSARFARTVLFVPPGVDADEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARARD 120

Query: 121 CPKDNKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGLFDFTFVLTIPYATGIYLWL 180
           CPKDNKPEFAILGRSNVGKSSLINALVRKKEVALTSKKP                     
Sbjct: 121 CPKDNKPEFAILGRSNVGKSSLINALVRKKEVALTSKKP--------------------- 180

Query: 181 IPLNFCLIAGKTQLINHFLVNKSWYIVDLPGYGFAKASETARMDWSSFTKGYFINRDTLV 240
                    GKTQLINHFLVNKSWYIVDLPGYGFAKASETARMDWSSFTKGYFINRDTLV
Sbjct: 181 ---------GKTQLINHFLVNKSWYIVDLPGYGFAKASETARMDWSSFTKGYFINRDTLV 240

Query: 241 AVLLLVDASVPPQKIDLDCANWLGRNNIPMTFVFTKCDKMKVSKGKRPDENIREFQQLIR 300
           AVLLLVDASVPPQKIDLDCANWLGRNNIP+TFVFTKCDKMKVSKGKRPDENIR+FQQLIR
Sbjct: 241 AVLLLVDASVPPQKIDLDCANWLGRNNIPLTFVFTKCDKMKVSKGKRPDENIRDFQQLIR 300

Query: 301 QNYKVHPSWIMTSSVTGLGRDELLLHMSQLRNYWDQ 337
           QNYKVHPSWIMTSSVTGLGRDELLLHMSQLRNYWDQ
Sbjct: 301 QNYKVHPSWIMTSSVTGLGRDELLLHMSQLRNYWDQ 306

BLAST of Lsi10G005170 vs. NCBI nr
Match: XP_022927961.1 (GTP-binding protein At2g22870 [Cucurbita moschata])

HSP 1 Score: 563.5 bits (1451), Expect = 1.2e-156
Identity = 290/336 (86.31%), Postives = 295/336 (87.80%), Query Frame = 0

Query: 1   MLLKSLSRLQNLPLASLFSPTLSPKSSPHFLSFSAPLSHSTPPNRSPSHLVSASNATAKT 60
           M+LK+LSRL  LPLASLFSPTLSPKSSPHFL  S+PLSHST PNRSPS L S SN TAKT
Sbjct: 1   MILKTLSRLHTLPLASLFSPTLSPKSSPHFLPVSSPLSHSTAPNRSPSLLASVSNTTAKT 60

Query: 61  SIYDSARFARTVLFVPPGVDADEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARARD 120
           SI DSARFARTVLFVPPGVDADEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARARD
Sbjct: 61  SISDSARFARTVLFVPPGVDADEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARARD 120

Query: 121 CPKDNKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGLFDFTFVLTIPYATGIYLWL 180
           CPKDNKPEFAILGRSNVGKSSLINALVRKKEVALTSKKP                     
Sbjct: 121 CPKDNKPEFAILGRSNVGKSSLINALVRKKEVALTSKKP--------------------- 180

Query: 181 IPLNFCLIAGKTQLINHFLVNKSWYIVDLPGYGFAKASETARMDWSSFTKGYFINRDTLV 240
                    GKTQLINHFLVNKSWYIVDLPGYGFAKASETAR DWSSFTKGYFINRDTLV
Sbjct: 181 ---------GKTQLINHFLVNKSWYIVDLPGYGFAKASETARTDWSSFTKGYFINRDTLV 240

Query: 241 AVLLLVDASVPPQKIDLDCANWLGRNNIPMTFVFTKCDKMKVSKGKRPDENIREFQQLIR 300
           AVLLLVDASVPPQKIDLDCANWLGRNNIP+TFVFTKCDKMKVSKGKRPDENIR+FQQLIR
Sbjct: 241 AVLLLVDASVPPQKIDLDCANWLGRNNIPLTFVFTKCDKMKVSKGKRPDENIRDFQQLIR 300

Query: 301 QNYKVHPSWIMTSSVTGLGRDELLLHMSQLRNYWDQ 337
           QNYKVHPSWIMTSSVTGLGRDELLLHMSQLRNYWDQ
Sbjct: 301 QNYKVHPSWIMTSSVTGLGRDELLLHMSQLRNYWDQ 306

BLAST of Lsi10G005170 vs. NCBI nr
Match: KAG7011050.1 (GTP-binding protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 563.1 bits (1450), Expect = 1.6e-156
Identity = 289/336 (86.01%), Postives = 295/336 (87.80%), Query Frame = 0

Query: 1   MLLKSLSRLQNLPLASLFSPTLSPKSSPHFLSFSAPLSHSTPPNRSPSHLVSASNATAKT 60
           M+LK+L+RL  LPLASLFSPTLSPKSSPHFL  S+PLSHST PNRSPS L S SN TAKT
Sbjct: 1   MILKTLTRLHTLPLASLFSPTLSPKSSPHFLPISSPLSHSTAPNRSPSLLASVSNTTAKT 60

Query: 61  SIYDSARFARTVLFVPPGVDADEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARARD 120
           SI DSARFARTVLFVPPGVDAD VSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARARD
Sbjct: 61  SISDSARFARTVLFVPPGVDADHVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARARD 120

Query: 121 CPKDNKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGLFDFTFVLTIPYATGIYLWL 180
           CPKDNKPEFAILGRSNVGKSSLINALVRKKEVALTSKKP                     
Sbjct: 121 CPKDNKPEFAILGRSNVGKSSLINALVRKKEVALTSKKP--------------------- 180

Query: 181 IPLNFCLIAGKTQLINHFLVNKSWYIVDLPGYGFAKASETARMDWSSFTKGYFINRDTLV 240
                    GKTQLINHFLVNKSWYIVDLPGYGFAKASETARMDWSSFTKGYFINRDTLV
Sbjct: 181 ---------GKTQLINHFLVNKSWYIVDLPGYGFAKASETARMDWSSFTKGYFINRDTLV 240

Query: 241 AVLLLVDASVPPQKIDLDCANWLGRNNIPMTFVFTKCDKMKVSKGKRPDENIREFQQLIR 300
           AVLLLVDASVPPQKIDLDCANWLGRNNIP+TFVFTKCDKMKVSKGKRPDENIR+FQQLIR
Sbjct: 241 AVLLLVDASVPPQKIDLDCANWLGRNNIPLTFVFTKCDKMKVSKGKRPDENIRDFQQLIR 300

Query: 301 QNYKVHPSWIMTSSVTGLGRDELLLHMSQLRNYWDQ 337
           QNYKVHPSWIMTSSVTGLGRDELLLHMSQLRNYWDQ
Sbjct: 301 QNYKVHPSWIMTSSVTGLGRDELLLHMSQLRNYWDQ 306

BLAST of Lsi10G005170 vs. NCBI nr
Match: KAG6571274.1 (GTP-binding protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 561.2 bits (1445), Expect = 5.9e-156
Identity = 288/336 (85.71%), Postives = 294/336 (87.50%), Query Frame = 0

Query: 1   MLLKSLSRLQNLPLASLFSPTLSPKSSPHFLSFSAPLSHSTPPNRSPSHLVSASNATAKT 60
           M+LK+L+RL  LPLASLFSPTLSPKSSPHFL  S+PLSHST PNRSPS L S SN TAK 
Sbjct: 1   MILKTLTRLHTLPLASLFSPTLSPKSSPHFLPISSPLSHSTAPNRSPSLLASVSNTTAKA 60

Query: 61  SIYDSARFARTVLFVPPGVDADEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARARD 120
           SI DSARFARTVLFVPPGVDAD VSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARARD
Sbjct: 61  SISDSARFARTVLFVPPGVDADHVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARARD 120

Query: 121 CPKDNKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGLFDFTFVLTIPYATGIYLWL 180
           CPKDNKPEFAILGRSNVGKSSLINALVRKKEVALTSKKP                     
Sbjct: 121 CPKDNKPEFAILGRSNVGKSSLINALVRKKEVALTSKKP--------------------- 180

Query: 181 IPLNFCLIAGKTQLINHFLVNKSWYIVDLPGYGFAKASETARMDWSSFTKGYFINRDTLV 240
                    GKTQLINHFLVNKSWYIVDLPGYGFAKASETARMDWSSFTKGYFINRDTLV
Sbjct: 181 ---------GKTQLINHFLVNKSWYIVDLPGYGFAKASETARMDWSSFTKGYFINRDTLV 240

Query: 241 AVLLLVDASVPPQKIDLDCANWLGRNNIPMTFVFTKCDKMKVSKGKRPDENIREFQQLIR 300
           AVLLLVDASVPPQKIDLDCANWLGRNNIP+TFVFTKCDKMKVSKGKRPDENIR+FQQLIR
Sbjct: 241 AVLLLVDASVPPQKIDLDCANWLGRNNIPLTFVFTKCDKMKVSKGKRPDENIRDFQQLIR 300

Query: 301 QNYKVHPSWIMTSSVTGLGRDELLLHMSQLRNYWDQ 337
           QNYKVHPSWIMTSSVTGLGRDELLLHMSQLRNYWDQ
Sbjct: 301 QNYKVHPSWIMTSSVTGLGRDELLLHMSQLRNYWDQ 306

BLAST of Lsi10G005170 vs. TAIR 10
Match: AT2G22870.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 426.8 bits (1096), Expect = 1.6e-119
Identity = 211/318 (66.35%), Postives = 247/318 (77.67%), Query Frame = 0

Query: 21  TLSPKSSPHFLSFSAPLSHSTPPNRSPSHLVSASNATAKT--SIYDSARFARTVLFVPPG 80
           TL    +P+F+S              P    S +N T  T  SI D+ +FA++VLF+PPG
Sbjct: 26  TLQSFRNPNFISI-------------PKISASTNNPTTTTNRSISDATKFAKSVLFIPPG 85

Query: 81  VDADEVSEEMILPGSNIVLGPYAGDSRVKEVEFVKSSARARDCPKDNKPEFAILGRSNVG 140
           V+ +E++++M+LPGSNIV+GP+AG S++KEVEFVKSSARARDCPKD++PE AILGRSNVG
Sbjct: 86  VEIEELTDDMVLPGSNIVIGPFAGHSQIKEVEFVKSSARARDCPKDDRPEIAILGRSNVG 145

Query: 141 KSSLINALVRKKEVALTSKKPGLFDFTFVLTIPYATGIYLWLIPLNFCLIAGKTQLINHF 200
           KSSLIN LVRKKEVALTSKKP                              GKTQLINHF
Sbjct: 146 KSSLINCLVRKKEVALTSKKP------------------------------GKTQLINHF 205

Query: 201 LVNKSWYIVDLPGYGFAKASETARMDWSSFTKGYFINRDTLVAVLLLVDASVPPQKIDLD 260
           LVNKSWYIVDLPGYGFAK S+ A+ DWS+FTKGYF+NRD+LV VLLL+DASVPPQKIDLD
Sbjct: 206 LVNKSWYIVDLPGYGFAKVSDAAKTDWSAFTKGYFLNRDSLVCVLLLIDASVPPQKIDLD 265

Query: 261 CANWLGRNNIPMTFVFTKCDKMKVSKGKRPDENIREFQQLIRQNYKVHPSWIMTSSVTGL 320
           CANWLGRNN+PMTFVFTKCDKMK +KGKRPDENI+ FQQ+IR+N+KVHP WI+TSSV+GL
Sbjct: 266 CANWLGRNNVPMTFVFTKCDKMKATKGKRPDENIKAFQQIIRENFKVHPPWILTSSVSGL 300

Query: 321 GRDELLLHMSQLRNYWDQ 337
           GRDELLLHMSQLRNYWDQ
Sbjct: 326 GRDELLLHMSQLRNYWDQ 300

BLAST of Lsi10G005170 vs. TAIR 10
Match: AT5G11480.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 288.1 bits (736), Expect = 9.0e-78
Identity = 169/346 (48.84%), Postives = 213/346 (61.56%), Query Frame = 0

Query: 6   LSRLQNLPLASLFSPTLSPKSSPHFLSFSAPLSHSTPPNRSPSHLVSASN-ATAK---TS 65
           L+ L    L+ L  P+ S  SS HF SF  P   S   +R+     S SN AT +    S
Sbjct: 3   LAHLPRFHLSILAKPSFS-LSSSHF-SFLNP-KPSVTVSRTLFSFASKSNLATVEPIPLS 62

Query: 66  IYDS-------ARFARTVLFVPPGVD-ADEVSEEM---ILPGSNIVLGPYAGDSRVKEVE 125
           + DS          A   LF+PP  D + E S  +   IL GSNIVL  YA D++V + +
Sbjct: 63  VSDSTDLDDAPVEIALDKLFIPPETDISGEDSASLTTRILKGSNIVLSKYARDAQVVQAD 122

Query: 126 FVKSSARARDCPKDNKPEFAILGRSNVGKSSLINALVRKKEVALTSKKPGLFDFTFVLTI 185
           +VKSS +  DCP D  PEFA++GRSNVGKSSL+N+LVR+K +ALTSKKP           
Sbjct: 123 YVKSSVKTEDCPADGLPEFALVGRSNVGKSSLLNSLVRRKRLALTSKKP----------- 182

Query: 186 PYATGIYLWLIPLNFCLIAGKTQLINHFLVNKSWYIVDLPGYGFAKASETARMDWSSFTK 245
                              GKTQ INHF +N  WY+VDLPGYG+A A    + DW+ FTK
Sbjct: 183 -------------------GKTQCINHFRINDKWYLVDLPGYGYASAPHELKQDWNKFTK 242

Query: 246 GYFINRDTLVAVLLLVDASVPPQKIDLDCANWLGRNNIPMTFVFTKCDKMKVSK--GKRP 305
            YF+NR TLV+V LLVDAS+P + IDL+ A+WLG+N +PMT +FTKCDK K  K  GK+P
Sbjct: 243 DYFLNRSTLVSVFLLVDASIPVKPIDLEYASWLGQNQVPMTLIFTKCDKRKKKKNGGKKP 302

Query: 306 DENIREFQQLIRQNYKVHPSWIMTSSVTGLGRDELLLHMSQLRNYW 335
           + NI+EFQ LI+  ++  P WIMTSSVT  GRDE+LLHM+QLRNYW
Sbjct: 303 EANIKEFQDLIQGFFETTPPWIMTSSVTNQGRDEILLHMAQLRNYW 315

BLAST of Lsi10G005170 vs. TAIR 10
Match: AT5G58370.2 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 98.2 bits (243), Expect = 1.3e-20
Identity = 74/254 (29.13%), Postives = 109/254 (42.91%), Query Frame = 0

Query: 87  EMILPGSNIVLGPYAGDSRVKE------VEFVKSSARARDCPKDNKPEFAILGRSNVGKS 146
           E+  P  NI         R++E      +EF  ++  +   P  + PE A  GRSNVGKS
Sbjct: 243 ELPAPLDNIPQSTSTERERIEESVFRNKLEFFAAANVSSSFPPPDVPEIAFAGRSNVGKS 302

Query: 147 SLINALVRKKEVALTSKKPGLFDFTFVLTIPYATGIYLWLIPLNFCLIAGKTQLINHFLV 206
           SL+NAL R+  V  TS KPGL                              TQ IN F +
Sbjct: 303 SLLNALTRQWGVVRTSDKPGL------------------------------TQTINFFGL 362

Query: 207 NKSWYIVDLPGYGFAKASETARMDWSSFTKGYFINRDTLVAVLLLVDASVPPQKIDLDCA 266
                +VDLPGYGFA A +  +  W    K Y   R +L  V LLVD     +  D +  
Sbjct: 363 GPKVRLVDLPGYGFAFAKDEVKEAWEDLVKEYVSTRTSLKRVCLLVDTKWGMKPRDQELI 422

Query: 267 NWLGRNNIPMTFVFTKCDKM-KVSKGKRPDENIREFQQLIRQNYKVHPSWIMTSSVTGLG 326
           N + R+N     V TK D +  +   +R      + ++ ++ N  +    +M SS +G G
Sbjct: 423 NLMERSNTKYQIVLTKTDVVFPIDVARR----AMQIEEKLKANRSIVQPLMMVSSRSGAG 462

Query: 327 RDELLLHMSQLRNY 334
              L   ++++  +
Sbjct: 483 IGSLRTALAKIARF 462

BLAST of Lsi10G005170 vs. TAIR 10
Match: AT5G58370.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 93.6 bits (231), Expect = 3.3e-19
Identity = 66/198 (33.33%), Postives = 85/198 (42.93%), Query Frame = 0

Query: 87  EMILPGSNIVLGPYAGDSRVKE------VEFVKSSARARDCPKDNKPEFAILGRSNVGKS 146
           E+  P  NI         R++E      +EF  ++  +   P  + PE A  GRSNVGKS
Sbjct: 243 ELPAPLDNIPQSTSTERERIEESVFRNKLEFFAAANVSSSFPPPDVPEIAFAGRSNVGKS 302

Query: 147 SLINALVRKKEVALTSKKPGLFDFTFVLTIPYATGIYLWLIPLNFCLIAGKTQLINHFLV 206
           SL+NAL R+  V  TS KPGL                              TQ IN F +
Sbjct: 303 SLLNALTRQWGVVRTSDKPGL------------------------------TQTINFFGL 362

Query: 207 NKSWYIVDLPGYGFAKASETARMDWSSFTKGYFINRDTLVAVLLLVDASVPPQKIDLDCA 266
                +VDLPGYGFA A +  +  W    K Y   R +L  V LLVD     +  D +  
Sbjct: 363 GPKVRLVDLPGYGFAFAKDEVKEAWEDLVKEYVSTRTSLKRVCLLVDTKWGMKPRDQELI 410

Query: 267 NWLGRNNIPMTFVFTKCD 279
           N + R+N     V TK D
Sbjct: 423 NLMERSNTKYQIVLTKTD 410

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O810042.3e-11866.35GTP-binding protein At2g22870 OS=Arabidopsis thaliana OX=3702 GN=EMB2001 PE=2 SV... [more]
A0M5371.3e-4136.84Probable GTP-binding protein EngB OS=Gramella forsetii (strain KT0803) OX=411154... [more]
A5FIZ11.5e-4038.05Probable GTP-binding protein EngB OS=Flavobacterium johnsoniae (strain ATCC 1706... [more]
A6LBY43.3e-4038.77Probable GTP-binding protein EngB OS=Parabacteroides distasonis (strain ATCC 850... [more]
Q5LDL24.7e-3937.33Probable GTP-binding protein EngB OS=Bacteroides fragilis (strain ATCC 25285 / D... [more]
Match NameE-valueIdentityDescription
A0A6J1J7018.9e-15886.61GTP-binding protein At2g22870 OS=Cucurbita maxima OX=3661 GN=LOC111484051 PE=3 S... [more]
A0A6J1EIY55.8e-15786.31GTP-binding protein At2g22870 OS=Cucurbita moschata OX=3662 GN=LOC111434809 PE=3... [more]
A0A5D3BI847.1e-14781.55GTP-binding protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G... [more]
A0A1S3C6C37.1e-14781.55GTP-binding protein At2g22870 OS=Cucumis melo OX=3656 GN=LOC103497360 PE=3 SV=1[more]
A0A6J1D8D56.2e-14379.88GTP-binding protein At2g22870 OS=Momordica charantia OX=3673 GN=LOC111017869 PE=... [more]
Match NameE-valueIdentityDescription
XP_038900534.14.0e-16088.10GTP-binding protein At2g22870 [Benincasa hispida][more]
XP_022986232.11.8e-15786.61GTP-binding protein At2g22870 [Cucurbita maxima][more]
XP_022927961.11.2e-15686.31GTP-binding protein At2g22870 [Cucurbita moschata][more]
KAG7011050.11.6e-15686.01GTP-binding protein, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6571274.15.9e-15685.71GTP-binding protein, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT2G22870.11.6e-11966.35P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT5G11480.19.0e-7848.84P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT5G58370.21.3e-2029.13P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT5G58370.13.3e-1933.33P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 101..333
e-value: 1.2E-51
score: 177.1
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 118..329
IPR006073GTP binding domainPFAMPF01926MMR_HSR1coord: 129..160
e-value: 6.3E-8
score: 32.7
coord: 189..276
e-value: 2.4E-7
score: 30.8
NoneNo IPR availablePANTHERPTHR11649MSS1/TRME-RELATED GTP-BINDING PROTEINcoord: 48..336
NoneNo IPR availablePANTHERPTHR11649:SF75PROTEIN, PUTATIVE, EXPRESSED-RELATEDcoord: 48..336
IPR030393EngB-type guanine nucleotide-binding (G) domainPROSITEPS51706G_ENGBcoord: 125..333
score: 29.855093
IPR030393EngB-type guanine nucleotide-binding (G) domainCDDcd01876YihA_EngBcoord: 128..330
e-value: 1.70868E-64
score: 199.659
IPR019987GTP-binding protein, ribosome biogenesis, YsxCHAMAPMF_00321GTPase_EngBcoord: 114..331
score: 21.438562

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi10G005170.1Lsi10G005170.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005525 GTP binding
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0046872 metal ion binding