Lsi10G005050 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi10G005050
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionTranslation initiation factor 3 family protein, putative isoform 1
Locationchr10: 7199511 .. 7205260 (-)
RNA-Seq ExpressionLsi10G005050
SyntenyLsi10G005050
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCAGTTTCTCCATTGGCTCGAGCTTTGCTCGTGGAGAAGCTCTGCAACTGCGACTGGCGCGAGCCACCGTCTTGTTCGTTTGAAGGAAGCCTGGAAGGAGTGTTAAATCCATCGATCTTCGTACAAAATTTGCGCCCTAAGATGATTCTATGGTTGGGTCATTGAAATTAGGATACGATGACATTCTTCTGTAGAATAAACCAATCCAAGCTCAAGTACCTTTCGGATCAATTCAAGAGACATTATTTTCAATTCTCTTATCCTGCTTCGCTTGATTCCGGTCGTACTACGAAGCATGATTCTGGGTATCTAGGGCGGATAATTCCCCGCAGACCAGCTGATTTTGCCGATAATGTTAGGTTCTATGCTGCTCCGGTTCAGGTAGTGATTCTGATCCTTATGGATGAGTAATGGCGCATTGGTTTTTGAACTTGAGTTAATTTATATGAAACCTTGTGTAGGGGTACTTTTTTTCTTTTTCTTTTTTCTTTTTCTTTTTTCTTTTTCTACTTCGTTTAAGGTTTCAAATTTTGTTTTAGTCGTTAAAATTTCATGCCTAGAAACTTTTCAGAAGTTACATATTAGAATAGGGAACAAAATTAGATTTGAATTATTAATTTCTAATGTCATATTGGTTTTCGCTAAAAAATTTACTATTAACGGGTTATGTTCGAATTCAGCACTGGTTTTAAATTAGGCAGGTTGTTTTAATTATAGGCTGTTTAGTTTATGGTGACTGATGTTGTTCACTCTATATGACATGTTGTTGAAGTTTCAAGGTAAGGCGAAGGAAGAGAAGGACACGAGTGGGCCGCGACTTAATGATCAAATTAAGGCTGATTTTGTGAGGCTTGTACATGATGATGGTATGTAGGATAAAAAATATTGAATTTTTGTTAATATCATAGTTTGAGATTCCCAAGTTGCGATGCGTTTTCACGTTCTTAAGATTGGTACCACGTTCATAGTGATGCATCTAGTGGTTAGCATAGCTCTCTTCCCTCCGATAAAATGGGAAGAGTGCATGTAACATTAATTTATCATATTTTGGGGAAGAAAGGTGTGTGTTTACTGTTTGATAAGTATTTTAGTTTTTTCTTCATGTCTGATATTTTATGTACAACGACATACTATATAGATAAAATTTGCTAGAAAGCAAGTATTGCATCTATCAATTGAAATGCGTGATGGGTTATGCTAACTTGTTTGTGGAAAAATAGTAGAGCATGACAATAATGGAAGCATGAGTAAGAAAATAAATGTACATTTTTTTAAGAGAAGGAAGAAGTATAATTTGGACAAGAAGGAGGGTTTCCTCCTTATGGGGCGAAGGAATTGAACAACATTCTCCATTTGACGTGAATGTCAAAAGCAGGATTAGTATGAAAAGTTCTTTTAGGAGACACTATAAACGAAACCTTAAAATAGATTCTACTAAAGAAAAAAAACTGTACTGAAATTTTTCTTTAAAGGTCATACTATCTCTTTAAACCAACTAGCCAAGCAAGAATCAATTCATCCACCATCACCAGTCTCCAAAAAATCTTAGAGAATGGATTTTCTCATGTAGAGAAGGTGTGTAAAAATGTATCGGCTTTCATTATGCATGTGGATTATGTTTATTTATTACTTGTTGACGTGTTGCACATATTTTTGTAGGACATACAATTCTTTCGCTACATGAAGCTCTTGAACGTGCTCGTGATCTCAAGCTTGATTTGGTTGAGGTAAACTTCCAGTTTTAATGTTTCTATCTTATGTCGTCTTTTTATATTCATAGTTGTACTTTCAATCTTAGTAATTGATGAAGTCTTTCCTACTTATTATTAGGTCTTGCAAAGTTTGATTCCATGTGTCAAATATTTTTCAGGTTCAACAAAAAGCCAATCCACCTGTGTGTAAAATTATGGACTTCCACAGAGAGAAGTACAAACAGCAAATAAGGGAAAAGGATCGTGTTAAAAGCAAGGTGAGTTTCTCAAGGCTGTGTGCATATGGGATTGTGACTACTGTTAAGACAGTGATAATGCTTTTAGTCTAACTGGTTTTGTATTGTTGTTCATTTTGGTAATGAATGAAACGTGATATAAATTAGCCAAAAATGTGGATGATTGAACACATCTAACATTTTTGGACTACCTTCATGGTTGCATAAATAAAACACAGTCATATTTGGTTGTCCGAGCTTCATTATTAATCACATGAAAAAGATTATTCATTCACCTTGCCTTCGCTTTGTTAAGTTGGGTGCTTTGAAAAGTACAATGAATTTGTTGCAATAAATTTTGGCCTTTTTGAAGGCCACATGCAAATCTCTAGAGCTTTGGCGTAATTTCTTGAATGGCCATTTTGAACTTATGCCAGTTTTTTAGTTACCTTTTTCCCTGCAGGAAAACCTGTTAGTAGTAGTACTTCAGACCTTCAGAAATTACACCTTAAAAATGATATTAGAAAAAGTGTTGTACATATTGTGATACAAATTCATATTCTTGCAGTCTGAAGTGAGTTTAAAAACGGGAGATCACAAAGAAGTCCGCTTCACTGGGAAAACTGTAAGTAATTTATTTTTTAATGTTTAGACCTTTTGCTGCCTGGAATGTAACTTGAAGACTCGCTGGTAGTGATATGATTGAATGCCTTACTGATGAAACAGATTTTACTATCCCAGTCTCTATCTCTCCCCACCTCTATAGTAAAACTAAATTGAGTACAAAAGTCCCAAGTATGACCATAATTGTTGTGTTTTAAACTATGGTTTGTACTCTAGTTCACGTTCACTCGTGAAAGTTTTGTACCATCAGTTCTTTAAAATGACTTGATAGCTCAAGATAAATATGTTCTTAAAGGATTATTAGTTCATCTTTATCATTTTTTGTAATGCACTTTTTAGGCGTTCGAAAATCAAGTGGCCAAAAATGTTAACCAACTTATAATTGAGGTGACCACTTAAGGTATCCATCCTAATTCCGGGAAGGATTATATATACCAAACTTTTATTGACAAAACATGTTTGTGGATAGAAGCATTTAAAATTCAAAGGTGGATATCTTCATCAACCCATTTGTCATTACCTGACGTCTAAAAGTAAAAAATTTGAAATGTAGGCAAAGATTAGTCATAGCTATATTTTTGGTTTTTTTCCCCTCACCGAATGATTGTTGAGGATTTGGTTCCTTTCTCTCTTTGACACCATTTTCTGGTAATTTATGCAAGTTGAGTCACAACTCTGTTTCCGAGCTCCAAATCAACGGCCTTTTGCCCTCTATGAGGGCTTAGAAAAACAACTTTTGGTATTTGATGCTAAAACCTTTTTTTTTTGTTTTAATTTTTAAAATCTGATCTTCAGGAGGAGAAGGACTTAAGGATGAAAGCTGATATGGTAAAGCGATTGATGGAACGTGGGTACCGTGTGAAGGTATTACTTTTCCATATTACAAAGTAACTCCTTATAATTTCTGTTCTTTTAGTTTCTTATTTCTTTTCAGAGGTTAATAGTCGTTTGTTTGTGTTTTTTTCGATACAAAATCCCCTCAGAATGGCCTAGCCCATCTTGCCTTTTTCTTTCCTGATTTAGAATTGCTGACTGTTACAATTCCCTAATCCCTATAACAAACTACAACCACGTCATTAACTATTGACCTAGTTACCAACCCTTATCCAGACCCTGACCTAAGATATTTCACCAGCTTCGTTGTAGTTCTAATTTCACTAAATTTTCAGTTGCGACATCCGGTTCTCATTTTCTATCCTTTATTTGTTTTTGAAGTATCCTCATTTAGATGGGGTGTCTTTGACTACTCTCGATTTGCCTAGTGGTCAATAAGAGCCATGAAAATAATAAAGAGCTCGGAAGAAATGAGTAATAGCCTTGGTAACCACTTACCTAAGTTTTAATATCCTACGAGTTTTTTTGGCAACTAAATGTTGTAGGGTAAAGTAGTTTGTCTCGTAAGAATAGTTGAGGTGCTCTTTTTTTGTAATTTAATATCATCAATTCGATTGTTTCCTTTTCAAAAAAGAGAGTGGTCGAGCCCAAACACTCACGGATATTAAAAAAAGAGAATAGTGGAGGTGCTTACAAACTGTCTTGAACCCTCACAAATATTTAAAAATAAAATAAAATGTGGTGTCCTATGGTATAAGGCCATGCCATTTGTGTGTAAAGATCATGACTATTTGCTTGTTCTATTTGTACAATCCTTGTAATCACTTTCCAAGACCCCTCCGCTTTGTGAAACGTACGATTGCTGATCTTAGTTGTTCAAATTTGACGTTTTAAGCATCATGGTGTTCGATAGTAGCTTACTCTATATGTTCCCTGTACAGTGTAGTGCCAAGGGTCGTGAGAATGAGGACTTGGGTGACTTGTTATCTCGTCTCTCTGCTTTGGTATTGCCTCATGAACTACTTCATTTTCTCTCATAAGGATTCCAATTTATCAAATTTCTGTTAAGTGTCTTATTCTTCGCACGGCTTATCACTATTTTTTGCAGATTGAGGATGTGGCAGTGGTGGAAAGTGGACCAAGGGTGGATAAAGGGCAGGCATATGTTATAGTCAGGCATGTTAAATTTGGCCCCTTGAAGAAAGGGGGTGGCGGCAAAGCATTGAAAGTTGTTGAAAAAAAGAAACAGAAGGTTCAAAATGGCACCACCCCTCTGGTTTCAGCAGCCCGTTCTTCAAGCTCCGACGAAGTAGAAATGGGTCCTGAAGAATCAGAAACTGAAGATGATTTTGAGAGTTCTAAAAAGACTACCTGGTCATTTGTTGATGGTAATAAAGATTTTGACGAAGTGTTTGACTTGAAAGATGATGCCAATGGGATTCCCTCCACAGCCACTACCAAGAAAATGAATGTCGTTTCAGACAGGGATGTAAATGTCTCAGACCTTTCTAAATTAAGGCAGTTACAAGATTCTCCCATCACAGCCGATAATAGATACAAAAAGACTGCACCTACGAACCGTTTCCAACCATCAAATGTAGCAGACCGCAACAGCCGAGGTGCTAGAGATCCACTCCGATCAGAACCCGAGAATCGCGATCAGAGGAGGCACCCTCCTACAAACACAAACTTCTCACCCTCAATGAGAGAAGCCAGACGATATGATAATAATACTTCTGCATCAAGAAACAGTAATCATTCTTCAAATGAGACATCCAAGCCTGGTCCATTACAACATACCAATCCTGGTCCATCCAAAACTAGCTTTGGGATCTTCAGTTCTCTAAACTCCAATGCTCCAAGGAAGCAAGATGCACCAGCCACAAGTTATCAAGGCAATCGAGGGCCTCCATACACGCCATCGGCTAATCCTAATGCACCTGGAGCTGCAGGAAATACAAAGTTTTCGATAACGAACTCTGAAGACAATTTGAACAAGGGACAGAAAAGCTGGGGAATTTTCAGCAAGGGGAGTCCAAATGATAACCCAGACAGGATATCAAAATCTAACTAAGGTAAGTGGTAGAACATGCTTACATTAATTGTTTCAGATATTACATGGTAGGCTGAATTTTGATGAGGATATTTATCTAGACATAAGACAGAGCAGAGATTTTTGTTTCAGACAGGTACTGCTGTGCATCCCCTGTTTTAAATGGGCAACATAATTTTTTTCAAATGTATCAGTAATAAATTTAATTTCTTGTCATTGGAGATGTTTGGCAGCTTACTGATTCAGATCTTTACTCTTGTGGTTAAAAAATTTTAAGAGATGAGAATGAGCACATAGTTAATTTATTCCCT

mRNA sequence

CTCAGTTTCTCCATTGGCTCGAGCTTTGCTCGTGGAGAAGCTCTGCAACTGCGACTGGCGCGAGCCACCGTCTTGTTCGTTTGAAGGAAGCCTGGAAGGAGTGTTAAATCCATCGATCTTCGTACAAAATTTGCGCCCTAAGATGATTCTATGGTTGGGTCATTGAAATTAGGATACGATGACATTCTTCTGTAGAATAAACCAATCCAAGCTCAAGTACCTTTCGGATCAATTCAAGAGACATTATTTTCAATTCTCTTATCCTGCTTCGCTTGATTCCGGTCGTACTACGAAGCATGATTCTGGGTATCTAGGGCGGATAATTCCCCGCAGACCAGCTGATTTTGCCGATAATGTTAGGTTCTATGCTGCTCCGGTTCAGTTTCAAGGTAAGGCGAAGGAAGAGAAGGACACGAGTGGGCCGCGACTTAATGATCAAATTAAGGCTGATTTTGTGAGGCTTGTACATGATGATGGACATACAATTCTTTCGCTACATGAAGCTCTTGAACGTGCTCGTGATCTCAAGCTTGATTTGGTTGAGGTTCAACAAAAAGCCAATCCACCTGTGTGTAAAATTATGGACTTCCACAGAGAGAAGTACAAACAGCAAATAAGGGAAAAGGATCGTGTTAAAAGCAAGGAAAACCTGTTAGTAGTATCTGAAGTGAGTTTAAAAACGGGAGATCACAAAGAAGTCCGCTTCACTGGGAAAACTGAGGAGAAGGACTTAAGGATGAAAGCTGATATGGTAAAGCGATTGATGGAACGTGGGTACCGTGTGAAGTGTAGTGCCAAGGGTCGTGAGAATGAGGACTTGGGTGACTTGTTATCTCGTCTCTCTGCTTTGATTGAGGATGTGGCAGTGGTGGAAAGTGGACCAAGGGTGGATAAAGGGCAGGCATATGTTATAGTCAGGCATGTTAAATTTGGCCCCTTGAAGAAAGGGGGTGGCGGCAAAGCATTGAAAGTTGTTGAAAAAAAGAAACAGAAGGTTCAAAATGGCACCACCCCTCTGGTTTCAGCAGCCCGTTCTTCAAGCTCCGACGAAGTAGAAATGGGTCCTGAAGAATCAGAAACTGAAGATGATTTTGAGAGTTCTAAAAAGACTACCTGGTCATTTGTTGATGGTAATAAAGATTTTGACGAAGTGTTTGACTTGAAAGATGATGCCAATGGGATTCCCTCCACAGCCACTACCAAGAAAATGAATGTCGTTTCAGACAGGGATGTAAATGTCTCAGACCTTTCTAAATTAAGGCAGTTACAAGATTCTCCCATCACAGCCGATAATAGATACAAAAAGACTGCACCTACGAACCGTTTCCAACCATCAAATGTAGCAGACCGCAACAGCCGAGGTGCTAGAGATCCACTCCGATCAGAACCCGAGAATCGCGATCAGAGGAGGCACCCTCCTACAAACACAAACTTCTCACCCTCAATGAGAGAAGCCAGACGATATGATAATAATACTTCTGCATCAAGAAACAGTAATCATTCTTCAAATGAGACATCCAAGCCTGGTCCATTACAACATACCAATCCTGGTCCATCCAAAACTAGCTTTGGGATCTTCAGTTCTCTAAACTCCAATGCTCCAAGGAAGCAAGATGCACCAGCCACAAGTTATCAAGGCAATCGAGGGCCTCCATACACGCCATCGGCTAATCCTAATGCACCTGGAGCTGCAGGAAATACAAAGTTTTCGATAACGAACTCTGAAGACAATTTGAACAAGGGACAGAAAAGCTGGGGAATTTTCAGCAAGGGGAGTCCAAATGATAACCCAGACAGGATATCAAAATCTAACTAAGGTAAGTGGTAGAACATGCTTACATTAATTGTTTCAGATATTACATGGTAGGCTGAATTTTGATGAGGATATTTATCTAGACATAAGACAGAGCAGAGATTTTTGTTTCAGACAGGTACTGCTGTGCATCCCCTGTTTTAAATGGGCAACATAATTTTTTTCAAATGTATCAGTAATAAATTTAATTTCTTGTCATTGGAGATGTTTGGCAGCTTACTGATTCAGATCTTTACTCTTGTGGTTAAAAAATTTTAAGAGATGAGAATGAGCACATAGTTAATTTATTCCCT

Coding sequence (CDS)

ATGACATTCTTCTGTAGAATAAACCAATCCAAGCTCAAGTACCTTTCGGATCAATTCAAGAGACATTATTTTCAATTCTCTTATCCTGCTTCGCTTGATTCCGGTCGTACTACGAAGCATGATTCTGGGTATCTAGGGCGGATAATTCCCCGCAGACCAGCTGATTTTGCCGATAATGTTAGGTTCTATGCTGCTCCGGTTCAGTTTCAAGGTAAGGCGAAGGAAGAGAAGGACACGAGTGGGCCGCGACTTAATGATCAAATTAAGGCTGATTTTGTGAGGCTTGTACATGATGATGGACATACAATTCTTTCGCTACATGAAGCTCTTGAACGTGCTCGTGATCTCAAGCTTGATTTGGTTGAGGTTCAACAAAAAGCCAATCCACCTGTGTGTAAAATTATGGACTTCCACAGAGAGAAGTACAAACAGCAAATAAGGGAAAAGGATCGTGTTAAAAGCAAGGAAAACCTGTTAGTAGTATCTGAAGTGAGTTTAAAAACGGGAGATCACAAAGAAGTCCGCTTCACTGGGAAAACTGAGGAGAAGGACTTAAGGATGAAAGCTGATATGGTAAAGCGATTGATGGAACGTGGGTACCGTGTGAAGTGTAGTGCCAAGGGTCGTGAGAATGAGGACTTGGGTGACTTGTTATCTCGTCTCTCTGCTTTGATTGAGGATGTGGCAGTGGTGGAAAGTGGACCAAGGGTGGATAAAGGGCAGGCATATGTTATAGTCAGGCATGTTAAATTTGGCCCCTTGAAGAAAGGGGGTGGCGGCAAAGCATTGAAAGTTGTTGAAAAAAAGAAACAGAAGGTTCAAAATGGCACCACCCCTCTGGTTTCAGCAGCCCGTTCTTCAAGCTCCGACGAAGTAGAAATGGGTCCTGAAGAATCAGAAACTGAAGATGATTTTGAGAGTTCTAAAAAGACTACCTGGTCATTTGTTGATGGTAATAAAGATTTTGACGAAGTGTTTGACTTGAAAGATGATGCCAATGGGATTCCCTCCACAGCCACTACCAAGAAAATGAATGTCGTTTCAGACAGGGATGTAAATGTCTCAGACCTTTCTAAATTAAGGCAGTTACAAGATTCTCCCATCACAGCCGATAATAGATACAAAAAGACTGCACCTACGAACCGTTTCCAACCATCAAATGTAGCAGACCGCAACAGCCGAGGTGCTAGAGATCCACTCCGATCAGAACCCGAGAATCGCGATCAGAGGAGGCACCCTCCTACAAACACAAACTTCTCACCCTCAATGAGAGAAGCCAGACGATATGATAATAATACTTCTGCATCAAGAAACAGTAATCATTCTTCAAATGAGACATCCAAGCCTGGTCCATTACAACATACCAATCCTGGTCCATCCAAAACTAGCTTTGGGATCTTCAGTTCTCTAAACTCCAATGCTCCAAGGAAGCAAGATGCACCAGCCACAAGTTATCAAGGCAATCGAGGGCCTCCATACACGCCATCGGCTAATCCTAATGCACCTGGAGCTGCAGGAAATACAAAGTTTTCGATAACGAACTCTGAAGACAATTTGAACAAGGGACAGAAAAGCTGGGGAATTTTCAGCAAGGGGAGTCCAAATGATAACCCAGACAGGATATCAAAATCTAACTAA

Protein sequence

MTFFCRINQSKLKYLSDQFKRHYFQFSYPASLDSGRTTKHDSGYLGRIIPRRPADFADNVRFYAAPVQFQGKAKEEKDTSGPRLNDQIKADFVRLVHDDGHTILSLHEALERARDLKLDLVEVQQKANPPVCKIMDFHREKYKQQIREKDRVKSKENLLVVSEVSLKTGDHKEVRFTGKTEEKDLRMKADMVKRLMERGYRVKCSAKGRENEDLGDLLSRLSALIEDVAVVESGPRVDKGQAYVIVRHVKFGPLKKGGGGKALKVVEKKKQKVQNGTTPLVSAARSSSSDEVEMGPEESETEDDFESSKKTTWSFVDGNKDFDEVFDLKDDANGIPSTATTKKMNVVSDRDVNVSDLSKLRQLQDSPITADNRYKKTAPTNRFQPSNVADRNSRGARDPLRSEPENRDQRRHPPTNTNFSPSMREARRYDNNTSASRNSNHSSNETSKPGPLQHTNPGPSKTSFGIFSSLNSNAPRKQDAPATSYQGNRGPPYTPSANPNAPGAAGNTKFSITNSEDNLNKGQKSWGIFSKGSPNDNPDRISKSN
Homology
BLAST of Lsi10G005050 vs. ExPASy Swiss-Prot
Match: Q6NLP2 (Translation initiation factor IF3-1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=IF3-1 PE=2 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 7.4e-42
Identity = 170/515 (33.01%), Postives = 250/515 (48.54%), Query Frame = 0

Query: 7   INQSKLKYLSDQFKRHYFQFSYPASLDS--GRTTKHDSGYL-GRIIPRRPADFADNVRFY 66
           IN+S LKY S+Q  R+Y+     AS      +TTK  S  +    I  RP++   N+RF 
Sbjct: 7   INRSYLKYASNQLTRNYYTQVCLASSTHVVKQTTKLSSFDIPNSDICTRPSNIFQNLRFL 66

Query: 67  AAPVQFQGKAKEEKDTSGPRLNDQIKADFVRLVHDDGHTILSLHEALERARDLKLDLVEV 126
           A   Q + K + E D+ GPRLN++I  D+VRLV ++GH ++SL EAL RA++L+ DLVEV
Sbjct: 67  ATSAQTR-KKEAEVDSDGPRLNEKITGDYVRLVSEEGHCVVSLREALRRAKELQCDLVEV 126

Query: 127 QQKANPPVCKIMDFHREKYKQQIREKDRVKSKENLLVVSEVSLKTGDHKEVRFTGKTEEK 186
           Q+ A PPVCKI+ +  E YK+    K+R K+K    +  ++       KE+RFT K E K
Sbjct: 127 QRDAKPPVCKIVKYSLELYKKAKVGKERAKAKRAEAIRPDI-------KEIRFTPKIEAK 186

Query: 187 DLRMKADMVKRLMERGYRVKCSA-------KGRENEDLGDLLSRLSALIEDVAVVESGPR 246
           DL+ K+D   +LME GYRVKC A       K  E E L +LL R +  I D A+VES P 
Sbjct: 187 DLKFKSDQALKLMESGYRVKCLAVPDKDKHKELEPEKLLELLFRFTCFIGD-ALVESWPE 246

Query: 247 VDKGQAYVIVRHVKFGPLKKGGGGKALKVVEKKKQKVQNGTTPLVSAARSSSSDEVEMGP 306
            D+  A VIVRH KFGP KK GG K +K ++ K  +V+  +    S+    ++ + +   
Sbjct: 247 ADRKGAVVIVRHAKFGPPKK-GGVKLMKDIDIKSARVKEESPKPDSSKAGVATVDDQEDI 306

Query: 307 EESETEDDFESSKKTTWSFVDGNKDFDEVFDLKDDANGIPSTATTKKMNVVSDRDVNVSD 366
           E+SE     E ++   +      ++    F    DA+     +  + +N    R  N + 
Sbjct: 307 EKSEPRFSVEQAQPVKFQNAYAKREPSSEFSGGRDASRFEPQSPNQHVNPQRPRFSNQAP 366

Query: 367 LSKLR-----QLQDSPITADN-RYKKTAPTNRFQPSNVADRNSRGARDPLRSE-PENRDQ 426
             +       Q  + P +A   ++    PT RF P       S+  R    ++ P  +  
Sbjct: 367 NQQPTGRFDPQSPNQPPSAPRPQFPNQQPTGRFDP----QFPSQPPRPQFPNQAPNQQST 426

Query: 427 RRHPPTNTNFSPSMREARRYDNNTSASRNSNHSSNETSKPG-PLQHTNPGPSKTSFGIFS 486
            R  P   N  PS  ++R  D   +   +    +    + G P +  N  P++   G F 
Sbjct: 427 GRFNPQFPNQRPSPPQSRFPDQAPNQQPSGPSPNRHPDRQGPPPRFQNQAPNQQPTGRFE 486

Query: 487 SLNSNAPRKQDAPATSYQGNRGPPYTPSANPNAPG 504
               N PR    P T     R P  T +  P APG
Sbjct: 487 PQPPNPPRAPPRPQT-----RLPNETSNEQPTAPG 502

BLAST of Lsi10G005050 vs. ExPASy Swiss-Prot
Match: Q5LQ58 (Translation initiation factor IF-3 OS=Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3) OX=246200 GN=infC PE=3 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 6.7e-19
Identity = 66/189 (34.92%), Postives = 112/189 (59.26%), Query Frame = 0

Query: 73  AKEEKDTSGPRLNDQIKADFVRLVHDDGHTILSLH--EALERARDLKLDLVEVQQKANPP 132
           A  ++DT GPR+ND+I+A  +RL+  DG  +  +H  +A+  A +  LDLVE+   A PP
Sbjct: 8   APPQRDT-GPRVNDKIRASEIRLIGADGENVGVVHPAKAMAMAEEAGLDLVEISPNATPP 67

Query: 133 VCKIMDFHREKYKQQIREKDRVKSKENLLVVSEVSLKTGDHKEVRFTGKTEEKDLRMKAD 192
           VCKIMDF + KY+QQ RE +  + K+ ++ V          KEV+F   T+  D  +K  
Sbjct: 68  VCKIMDFGKYKYEQQKRESE-ARKKQKIIEV----------KEVKFRPNTDTHDYEVKMR 127

Query: 193 MVKRLMERGYRVKCSA--KGRE--NEDLG-DLLSRLSALIEDVAVVESGPRVDKGQAYVI 252
            V + +E G +VK +   +GRE  +++LG +LL R++  ++++  +E+ P+++  Q  ++
Sbjct: 128 NVYKFLENGDKVKVTLRFRGREMAHQNLGRELLERVAEDVKEMGKIENMPKMEGRQMIMM 179

Query: 253 VRHVKFGPL 255
           +     GPL
Sbjct: 188 I-----GPL 179

BLAST of Lsi10G005050 vs. ExPASy Swiss-Prot
Match: Q8PJE2 (Translation initiation factor IF-3 OS=Xanthomonas axonopodis pv. citri (strain 306) OX=190486 GN=infC PE=3 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 6.3e-17
Identity = 60/176 (34.09%), Postives = 103/176 (58.52%), Query Frame = 0

Query: 78  DTSGPRLNDQIKADFVRLVHDDGHT--ILSLHEALERARDLKLDLVEVQQKANPPVCKIM 137
           D    R N +I+   VR++  DG    +LS  EAL +A +  LDLVE+Q +A+PPVCKIM
Sbjct: 12  DNKQNRKNQEIRVPRVRVIGSDGEMVGVLSRDEALAKAEEEGLDLVEIQPQADPPVCKIM 71

Query: 138 DFHREKYKQQIREKDRVKSKENLLVVSEVSLKTGDHKEVRFTGKTEEKDLRMKADMVKRL 197
           DF + K++QQ +  +  K  + + +           KE++F   T+E D ++K   ++R 
Sbjct: 72  DFGKFKFEQQKKANEAKKKTKQVEI-----------KELKFRPVTDEGDYQIKLRNMRRF 131

Query: 198 MERGYRVKCSA--KGRE--NEDLG-DLLSRLSALIEDVAVVESGPRVDKGQAYVIV 247
           +E G +VK +   +GRE  +++LG ++ +R+ A + D  V+ES PR++  Q  +++
Sbjct: 132 LEEGDKVKVNIRFRGREMSHQELGREMAARIEADLGDDIVIESRPRLEGRQMVMMI 176

BLAST of Lsi10G005050 vs. ExPASy Swiss-Prot
Match: Q5QYN5 (Translation initiation factor IF-3 OS=Idiomarina loihiensis (strain ATCC BAA-735 / DSM 15497 / L2-TR) OX=283942 GN=infC PE=3 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 1.8e-16
Identity = 59/185 (31.89%), Postives = 111/185 (60.00%), Query Frame = 0

Query: 70  QGKAKEEKDTSGPRLNDQIK-ADFVRLVHDDGHT--ILSLHEALERARDLKLDLVEVQQK 129
           +G  + +K     R+N+QI   D VRL+  DG    ++S++EAL+ A +  +DLVE+   
Sbjct: 2   KGGKRTQKAADKNRINEQITGVDEVRLIGTDGEQAGVVSINEALDAAAEAGVDLVEMSPN 61

Query: 130 ANPPVCKIMDFHREKYKQQIREKDRVKSKENLLVVSEVSLKTGDHKEVRFTGKTEEKDLR 189
           A PPVC++MD+ +  +++   +K++ K ++ + V           KEV+F   T+E D +
Sbjct: 62  AEPPVCRLMDYGKFLFEKSKEQKEQKKKQKQIQV-----------KEVKFRPGTDEGDYQ 121

Query: 190 MKADMVKRLMERGYRVKCSA--KGRE--NEDLG-DLLSRLSALIEDVAVVESGPRVDKGQ 247
           +K   ++R +E G + K +   +GRE  +++LG +LL+R+   +E++++VES PR  +G+
Sbjct: 122 VKLRNLRRFLEGGDKTKVTIRFRGREMAHQELGIELLNRVKNDLEEISIVESFPRRAEGR 175

BLAST of Lsi10G005050 vs. ExPASy Swiss-Prot
Match: Q8P7Z3 (Translation initiation factor IF-3 OS=Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25) OX=190485 GN=infC PE=3 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 1.8e-16
Identity = 59/176 (33.52%), Postives = 103/176 (58.52%), Query Frame = 0

Query: 78  DTSGPRLNDQIKADFVRLVHDDGHT--ILSLHEALERARDLKLDLVEVQQKANPPVCKIM 137
           D    R N +I+   VR++  DG    +LS  EAL +A +  LDLVE+Q +A+PPVCKIM
Sbjct: 12  DNKQNRKNQEIRVPRVRVIGSDGEMVGVLSRDEALAKAEEEGLDLVEIQPQADPPVCKIM 71

Query: 138 DFHREKYKQQIREKDRVKSKENLLVVSEVSLKTGDHKEVRFTGKTEEKDLRMKADMVKRL 197
           DF + K++QQ +  +  K  + + +           KE++F   T+E D ++K   ++R 
Sbjct: 72  DFGKFKFEQQKKANEAKKKTKQVEI-----------KELKFRPVTDEGDYQIKLRNMRRF 131

Query: 198 MERGYRVKCSA--KGRE--NEDLG-DLLSRLSALIEDVAVVESGPRVDKGQAYVIV 247
           +E G +VK +   +GRE  +++LG ++ +R+ A + +  V+ES PR++  Q  +++
Sbjct: 132 LEEGDKVKVNIRFRGREMSHQELGREMAARIEADLGEDIVIESRPRLEGRQMVMMI 176

BLAST of Lsi10G005050 vs. ExPASy TrEMBL
Match: A0A6J1CFY9 (translation initiation factor IF3-1, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111011172 PE=3 SV=1)

HSP 1 Score: 745.0 bits (1922), Expect = 2.3e-211
Identity = 409/557 (73.43%), Postives = 447/557 (80.25%), Query Frame = 0

Query: 1   MTFFCRINQSKLKYLSDQFKRHYFQFSYPASLDSGRTTKHDSGYLGRIIPRRPADFADNV 60
           M FFCRINQSKLK LS+QFKRHYFQ SY +SL SGR  KH S  L R+IP RPAD    V
Sbjct: 1   MAFFCRINQSKLKLLSNQFKRHYFQISYASSLGSGRREKHVSENLERVIPIRPADLGGKV 60

Query: 61  RFYAAPVQFQGKA-KEEKDTSGPRLNDQIKADFVRLVHDDGHTILSLHEALERARDLKLD 120
           RFYAAPVQFQGKA KEEKDTSGPRLND+IKA+FVRLV DDGHTILSLHEALERAR+LKLD
Sbjct: 61  RFYAAPVQFQGKAKKEEKDTSGPRLNDKIKAEFVRLVTDDGHTILSLHEALERARELKLD 120

Query: 121 LVEVQQKANPPVCKIMDFHREKYKQQIREKDRVKSKENLLVVSEVSLKTGDHKEVRFTGK 180
           LVEVQQKANPPVCKIMDFHREKYKQQIREKDRVKSK      +EVSL+ GDHKEVRFTGK
Sbjct: 121 LVEVQQKANPPVCKIMDFHREKYKQQIREKDRVKSK------AEVSLRKGDHKEVRFTGK 180

Query: 181 TEEKDLRMKADMVKRLMERGYRVKCSAKGRENEDLGDLLSRLSALIEDVAVVESGPRVDK 240
           TEEKDLRMKADMVKRLMERGYRVKCSA+G E++DLG LLSRLSALIED AVVESGPRV++
Sbjct: 181 TEEKDLRMKADMVKRLMERGYRVKCSARGGEDQDLGGLLSRLSALIEDEAVVESGPRVER 240

Query: 241 GQAYVIVRHVKFGPLKKGGGGKALKVVEKKKQKVQNGTTPLVSAARSSSSDEVEMGPEES 300
           GQAYV+VRHVKFGP KKGGGGKA KVV   + K+QNGT PLVS AR+S + E EM PEES
Sbjct: 241 GQAYVVVRHVKFGPSKKGGGGKASKVVANPRLKIQNGTAPLVSVARASRTSEEEMDPEES 300

Query: 301 ETEDDFESSKKTTWSFVDGNKDFDEVFDLKDDANGIPSTATTKKMNVVSDRDVNVSDLSK 360
           ++E D E+SK+ TWS VDGN DFDEVFDLKDDAN IPS  T KKM+VVSDRDVN+SDLS+
Sbjct: 301 DSEADLENSKE-TWSAVDGNNDFDEVFDLKDDANRIPSKYTAKKMDVVSDRDVNISDLSQ 360

Query: 361 LRQL-------------QDSPITADNRYKKTAPTNRFQPSNVADRNSRGARDPLRSEPEN 420
           LR+L             QDS + A+NRYKK  P  RFQP+NV D   RGARD  RSE + 
Sbjct: 361 LRRLPDSGRTSSVPLSQQDSSVKAENRYKKIEPVTRFQPTNVMDNKGRGARDSHRSETQI 420

Query: 421 RDQRRHPPTNTNFSPSMREARRYDNNTSASRNSNHSSNETSKPGPLQHTNPGPSKTSFGI 480
           RDQRRH PTN NFSP MRE RR +NNTS  RN  H  N TSKP P  H +P PSKTSFGI
Sbjct: 421 RDQRRHRPTNPNFSPPMRETRREENNTSPLRNIKHPLNATSKPDPSHHADPSPSKTSFGI 480

Query: 481 FSSLNSNAPRKQDAPATSYQGNRGPPYTPSANPNAPGAAGNTKFSITNSEDNLNKGQKSW 540
           FSSLN+NAP KQDA ATSYQ NR PPY PS NP   GA+ NTKF  T S +N+NKG+KSW
Sbjct: 481 FSSLNANAPGKQDAAATSYQSNRCPPYAPSNNPYLHGASENTKFPATKSNENINKGKKSW 540

Query: 541 GIFSKGSPNDNPDRISK 544
           GIFSK S ND P+RISK
Sbjct: 541 GIFSKDSQNDPPNRISK 550

BLAST of Lsi10G005050 vs. ExPASy TrEMBL
Match: A0A6J1G8E4 (translation initiation factor IF3-1, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111451694 PE=3 SV=1)

HSP 1 Score: 715.7 bits (1846), Expect = 1.5e-202
Identity = 399/560 (71.25%), Postives = 442/560 (78.93%), Query Frame = 0

Query: 1   MTFFCRINQSKLKYLSDQFKRHYFQFSYPASLDSGRTTKHDSGYLGRIIPRRPADFADNV 60
           M FFCRINQSKL + S+QFKRHYFQ  Y  S+ SGR+TKHDS Y+ RIIPR PADF   V
Sbjct: 1   MAFFCRINQSKLTHFSNQFKRHYFQVCYAPSIGSGRSTKHDSEYIARIIPRGPADFGGKV 60

Query: 61  RFYAAPVQFQGKA-KEEKDTSGPRLNDQIKADFVRLVHDDGHTILSLHEALERARDLKLD 120
           RFYAAPVQFQGKA KEE +TSGPRLND+IKA+FVRLV DDGH I+SL EAL RAR+LKLD
Sbjct: 61  RFYAAPVQFQGKAKKEENNTSGPRLNDKIKAEFVRLVSDDGHAIVSLREALGRARELKLD 120

Query: 121 LVEVQQKANPPVCKIMDFHREKYKQQIREKDRVKSKENLLVVSEVSLKTGDHKEVRFTGK 180
           LVEVQQKANPPVCKIMDFHREKYKQQIREKDRVKSK      +E  L+ GDHKEVRFTGK
Sbjct: 121 LVEVQQKANPPVCKIMDFHREKYKQQIREKDRVKSK------AEGGLRKGDHKEVRFTGK 180

Query: 181 TEEKDLRMKADMVKRLMERGYRVKCSAKGRENEDLGDLLSRLSALIEDVAVVESGPRVDK 240
           TEEKDLRMK DMVKRLMERGYRVKCSA+G E++DLG LLSRLSALIEDVA+VESGPRV++
Sbjct: 181 TEEKDLRMKVDMVKRLMERGYRVKCSARGGEDQDLGGLLSRLSALIEDVAMVESGPRVER 240

Query: 241 GQAYVIVRHVKFGPLKKGGGGKALKVVEKKKQKVQNGTTPLVSAARSSSSDEVEMGPEES 300
           GQAYVIVRHVKFGP KKGGGGKA KV    +Q+V+NGTTPLV AARSS+ DE EMGPEES
Sbjct: 241 GQAYVIVRHVKFGPSKKGGGGKASKVAVNARQEVKNGTTPLVPAARSSNPDEEEMGPEES 300

Query: 301 ETEDDFESSKKTTWSFVDGNKDFDEVFDLKDDANGIPSTATTKKMNVVSDRDVNVSDLSK 360
           ++          TWS VDGN DFDEVFDLKDDA GIP   T  KMNV SDRDVNVSDLS 
Sbjct: 301 DS--------GATWSVVDGNNDFDEVFDLKDDAKGIPLETTANKMNVASDRDVNVSDLSP 360

Query: 361 LRQL-------------QDSPITADNRYKKTAPTNRFQPSNVADRNSRGARDPLRSEPEN 420
             +L             QDS   ADNRYKK  P NRFQP NV D   RGARD LRSEP+ 
Sbjct: 361 PTRLPDTSRTSSVPLSRQDSSTKADNRYKKIEPANRFQPPNVMDNKGRGARDSLRSEPQI 420

Query: 421 RDQRRHPPTNTNFS-PSMREARRYDNNTSASRNSNHSSNETSKPGPLQHTNPGPSKTSFG 480
           R+Q R P TNTN S PSMRE R  DN +SASRN+ H SNETSK G  QHT P PSKTSFG
Sbjct: 421 RNQWRQPLTNTNLSPPSMRETRPDDNVSSASRNTKHPSNETSKSGSSQHTVPDPSKTSFG 480

Query: 481 IFSSLNSNAPRKQDAPATSYQGNRGPPYTPSANPNAPGAAGNTKFSITNSEDNLNKGQKS 540
           IFS+LN+NAP KQDA +TSY+GNRG    PS NPNAPGA+ + KFS+T S++N+NKG KS
Sbjct: 481 IFSALNANAPVKQDAASTSYRGNRG----PSTNPNAPGASESLKFSLTKSDNNVNKGSKS 540

Query: 541 WGIFSKGSPNDNPDRISKSN 546
           WGIFS+G PN++P+RISK N
Sbjct: 541 WGIFSEG-PNNSPNRISKPN 541

BLAST of Lsi10G005050 vs. ExPASy TrEMBL
Match: A0A6J1I5H4 (translation initiation factor IF3-1, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111469788 PE=3 SV=1)

HSP 1 Score: 703.0 bits (1813), Expect = 9.9e-199
Identity = 392/559 (70.13%), Postives = 432/559 (77.28%), Query Frame = 0

Query: 1   MTFFCRINQSKLKYLSDQFKRHYFQFSYPASLDSGRTTKHDSGYLGRIIPRRPADFADNV 60
           M FFCRIN         QFKRHYFQ  Y +S+ SGRTTKH S Y+ RIIPR PADF   V
Sbjct: 1   MAFFCRIN---------QFKRHYFQVCYASSIGSGRTTKHVSEYISRIIPRGPADFGGKV 60

Query: 61  RFYAAPVQFQGKA-KEEKDTSGPRLNDQIKADFVRLVHDDGHTILSLHEALERARDLKLD 120
           RFYAAPVQFQGKA KEE +TSGPRLND+IKA+FVRLV DDGH ILSL EAL RAR+LKLD
Sbjct: 61  RFYAAPVQFQGKAKKEENNTSGPRLNDKIKAEFVRLVSDDGHAILSLREALGRARELKLD 120

Query: 121 LVEVQQKANPPVCKIMDFHREKYKQQIREKDRVKSKENLLVVSEVSLKTGDHKEVRFTGK 180
           LVEVQQKANPPVCKIMDFHREKYKQQIREKDRVKSK      +EV L+ GDHKEVRFTGK
Sbjct: 121 LVEVQQKANPPVCKIMDFHREKYKQQIREKDRVKSK------AEVGLRKGDHKEVRFTGK 180

Query: 181 TEEKDLRMKADMVKRLMERGYRVKCSAKGRENEDLGDLLSRLSALIEDVAVVESGPRVDK 240
           TEEKDLRMKADMVKRLME+GYRVKCSA+G E++DLG LLSRLSALIEDVA+VESGPRV++
Sbjct: 181 TEEKDLRMKADMVKRLMEQGYRVKCSARGGEDQDLGGLLSRLSALIEDVAMVESGPRVER 240

Query: 241 GQAYVIVRHVKFGPLKKGGGGKALKVVEKKKQKVQNGTTPLVSAARSSSSDEVEMGPEES 300
           GQAYVIVRHVKFGP KKGGGGKA KV    +Q+V NGTTPLV AARSS+ DE E GPEE 
Sbjct: 241 GQAYVIVRHVKFGPSKKGGGGKASKVAVSARQEVINGTTPLVPAARSSTPDEEEKGPEEP 300

Query: 301 ETEDDFESSKKTTWSFVDGNKDFDEVFDLKDDANGIPSTATTKKMNVVSDRDVNVSDLSK 360
           ++E         TWS VDGN DFDEVFDLKDDANGIP   T  KMNV SDRD NVSDLS 
Sbjct: 301 DSE--------ATWSVVDGNNDFDEVFDLKDDANGIPLETTANKMNVASDRDANVSDLSP 360

Query: 361 LRQL-------------QDSPITADNRYKKTAPTNRFQPSNVADRNSRGARDPLRSEPEN 420
             +L             QDS   ADNRYKK  P NRFQP NV D   RGARD LRS+P+ 
Sbjct: 361 PTRLPDTSRTSSVPLSRQDSSTKADNRYKKIEPANRFQPPNVTDNKGRGARDSLRSDPQI 420

Query: 421 RDQRRHPPTNTNFSPSMREARRYDNNTSASRNSNHSSNETSKPGPLQHTNPGPSKTSFGI 480
           R+QRR         P M+E R  DNN+SASRN  H SNETSK GP  HT+PGPSKTSFGI
Sbjct: 421 RNQRR--------QPLMKETRLDDNNSSASRNIKHPSNETSKSGPSHHTDPGPSKTSFGI 480

Query: 481 FSSLNSNAPRKQDAPATSYQGNRGPPYTPSANPNAPGAAGNTKFSITNSEDNLNKGQKSW 540
           FSSLN+NAP KQDA +TSY+GNRG PY PS NPN PGA+ + KFS+T S+DN+NKG KSW
Sbjct: 481 FSSLNANAPGKQDAASTSYRGNRGLPYPPSTNPNVPGASESLKFSLTKSDDNVNKGSKSW 527

Query: 541 GIFSKGSPNDNPDRISKSN 546
           GIFS+G PN++P+RISK N
Sbjct: 541 GIFSEG-PNNSPNRISKPN 527

BLAST of Lsi10G005050 vs. ExPASy TrEMBL
Match: A0A0A0LJ90 (IF3_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G270880 PE=3 SV=1)

HSP 1 Score: 672.5 bits (1734), Expect = 1.4e-189
Identity = 387/589 (65.70%), Postives = 423/589 (71.82%), Query Frame = 0

Query: 1   MTFFCRINQSKLKYLSDQFKRHYFQFSYPASLDSGRTTKHDSGYLGRIIPRRPADFADNV 60
           M FFCRI+QSKLK   DQFKRHYFQ S+ A+LDS RTT +  GY+ R IPRRPADFAD  
Sbjct: 1   MAFFCRISQSKLKSFLDQFKRHYFQISHSAALDSRRTTHNVYGYVER-IPRRPADFADKF 60

Query: 61  RFYAAPVQFQGKA-KEEKDTSGPRLNDQIKADFVRLVHDDGHTILSLHEALERARDLKLD 120
           RFYAAPVQFQG A KEEKD SGPRLND+IKADFVRLV DDGHTILSL +AL+RAR+LKLD
Sbjct: 61  RFYAAPVQFQGNAKKEEKDKSGPRLNDKIKADFVRLVSDDGHTILSLRDALKRARELKLD 120

Query: 121 LVEVQQKANPPVCKIMDFHREKYKQQIREKDRVKSKENLLVVSEVSLKTGDHKEVRFTGK 180
           LVEVQQKANPPVCK+MDFHREKYK+QIREKDRVKSK       E+ +K   HKEVRFTGK
Sbjct: 121 LVEVQQKANPPVCKLMDFHREKYKKQIREKDRVKSK------VEMVMKKAGHKEVRFTGK 180

Query: 181 TEEKDLRMKADMVKRLMERGYRVKCSAKGRENEDLGDLLSRLSALIEDVAVVESGPRVDK 240
           TEEKDLRMKADMVKRLMERGYRVKC+A+G EN+DLG LLSRL+ALIEDVA+VE+GP V+K
Sbjct: 181 TEEKDLRMKADMVKRLMERGYRVKCTARGGENQDLGALLSRLTALIEDVALVENGPSVEK 240

Query: 241 GQAYVIVRHVKFGPLKKGGGGKALKVVEKKKQKVQNGTTPLVSAARSSSSDEVEMGPEES 300
           GQA++IVRHVKFGP KKGGG KA KVV   +QKVQNG+TPLVS                 
Sbjct: 241 GQAFIIVRHVKFGPSKKGGGSKASKVVANAEQKVQNGSTPLVSTV--------------- 300

Query: 301 ETEDDFESSKKTTWSFVDGNKDFDEVFDLKDDANGIPSTATTKKMNVVSDRDVNVSDLSK 360
           + ED FESS++TTW+ VDG  D DE FDLKDD N I ST  TKKMNVVSDRDV  S  + 
Sbjct: 301 DPEDGFESSEETTWT-VDGTSDSDEAFDLKDDRNVITSTTATKKMNVVSDRDVPDSGRTS 360

Query: 361 LRQL--QDSPITADNRYKK----------------------------------------- 420
              L  QDS I  DNR KK                                         
Sbjct: 361 SVPLFQQDSSIKTDNRPKKIEPMNHFQPPNSQGARDSLRSGPQIREPVNRFQPPNATDRY 420

Query: 421 --------------TAPTNRFQPSNVADRNSRGARDPLRSEPENRDQRRHPPTNTNFSPS 480
                           P NRFQP NV DR SRGARD LRSEP+ RDQR HPP NTNFSP+
Sbjct: 421 SQGARDSLRSEPQIREPMNRFQPPNVTDRYSRGARDSLRSEPQIRDQRWHPPPNTNFSPT 480

Query: 481 MREARRYDNNTSASRNSNHSSNETSKPGPLQHTNPGPSKTSFGIFSSLNSNAPRKQDAPA 532
           MRE+R+YDNNTS SRN NHSSN TS P P  H++  PSKTSFGIFSS NSNAP KQD PA
Sbjct: 481 MRESRQYDNNTSVSRNINHSSNSTSTPAPSHHSDSSPSKTSFGIFSSSNSNAPGKQDPPA 540

BLAST of Lsi10G005050 vs. ExPASy TrEMBL
Match: A0A6J1FYA8 (translation initiation factor IF3-1, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111448446 PE=3 SV=1)

HSP 1 Score: 670.6 bits (1729), Expect = 5.5e-189
Identity = 382/545 (70.09%), Postives = 409/545 (75.05%), Query Frame = 0

Query: 1   MTFFCRINQSKLKYLSDQFKRHYFQFSYPASLDSGRTTKHDSGYLGRIIPRRPADFADNV 60
           M  FCRINQSKLK LSDQFKRHYFQ SY ASLDSGR TKH  G + R IPRRPAD   NV
Sbjct: 1   MAVFCRINQSKLKSLSDQFKRHYFQISYAASLDSGRITKHVFGCVERTIPRRPADCGGNV 60

Query: 61  RFYAAPVQFQGKA-KEEKDTSGPRLNDQIKADFVRLVHDDGHTILSLHEALERARDLKLD 120
           RF+AAPVQFQGKA KEEKDTSGPRLND+IKADFVRLV DDGHT+L LHEAL RAR+L LD
Sbjct: 61  RFFAAPVQFQGKAKKEEKDTSGPRLNDKIKADFVRLVSDDGHTVLKLHEALARARELNLD 120

Query: 121 LVEVQQKANPPVCKIMDFHREKYKQQIREKDRVKSKENLLVVSEVSLKTGDHKEVRFTGK 180
           LVEVQ KANPPVCKIMDFHREKYKQQIREKDRVKSK      +E+SLK GDHKEVRFTGK
Sbjct: 121 LVEVQTKANPPVCKIMDFHREKYKQQIREKDRVKSK------AELSLKKGDHKEVRFTGK 180

Query: 181 TEEKDLRMKADMVKRLMERGYRVKCSAKGRENEDLGDLLSRLSALIEDVAVVESGPRVDK 240
           TEEKDLRMKADMVKRLMERGYRVKCSA+G E++DLG LLSRLSALIEDVA+VESGPRV++
Sbjct: 181 TEEKDLRMKADMVKRLMERGYRVKCSARGGEDQDLGGLLSRLSALIEDVALVESGPRVER 240

Query: 241 GQAYVIVRHVKFGPLKKGGGGKALKVVEKKKQKVQNGTTPLVSAARSSSSDEVEMGPEES 300
           GQAYVIVRHVKFGP KKGGGGKA  VV   KQK QNGTT   S ARSSS  E EMGPE  
Sbjct: 241 GQAYVIVRHVKFGPSKKGGGGKASNVVANAKQKAQNGTTSPTSPARSSSPREEEMGPEV- 300

Query: 301 ETEDDFESSKKTTWSFVDGNKDFDEVFDLKDDANGIPSTATTKKMNVVSDRDVNVSDLSK 360
               DFESSK+TTWS VDGN DFDEVFDLKDDANG PS     KM        NVSDLS+
Sbjct: 301 ----DFESSKETTWSVVDGNNDFDEVFDLKDDANGTPSKTAAMKM--------NVSDLSQ 360

Query: 361 LRQL-------------QDSPITADNRYKKTAPTNRFQPSNVADRNSRGARDPLRSEPEN 420
            R L             QD  I ADNRYKK  P NRFQP N      RGA+D  RSEPE 
Sbjct: 361 PRGLPDSGRTSSVPLSRQDPSIKADNRYKKIEPVNRFQPPNAMGSKGRGAKDSFRSEPEI 420

Query: 421 RDQRRHPPTNTNFSPSMREARRYDNNTSASRNSNHSSNETSKPGPLQHTNPGPSKTSFGI 480
           RDQRRH P NT+FSP  RE RRY++N SASRN         KPGP  +T P PSKTSFGI
Sbjct: 421 RDQRRHTP-NTDFSPLTRETRRYEDNASASRN--------IKPGPPHNTAPSPSKTSFGI 480

Query: 481 FSSLNSNAPRKQDAPATSYQGNRGPPYTPSANPNAPGAAGNTKFSITNSEDNLNKGQKSW 532
           FSSL++NAP KQDAPATSY+GN+GPPYTPS NP+                   +KGQKSW
Sbjct: 481 FSSLSANAPGKQDAPATSYRGNQGPPYTPSTNPD-------------------DKGQKSW 498

BLAST of Lsi10G005050 vs. NCBI nr
Match: XP_038902038.1 (translation initiation factor IF3-1, mitochondrial [Benincasa hispida])

HSP 1 Score: 828.6 bits (2139), Expect = 3.2e-236
Identity = 448/547 (81.90%), Postives = 475/547 (86.84%), Query Frame = 0

Query: 1   MTFFCRINQSKLKYLSDQFKRHYFQFSYPASLDSGRTTKHDSGYLGRIIPRRPADFADNV 60
           M FFCRINQSKLK LS QFKRHYFQ S+ ASLD G TTKH  GY+ RIIPRRPADFADNV
Sbjct: 1   MAFFCRINQSKLKSLSAQFKRHYFQISHVASLDPGCTTKH-VGYVERIIPRRPADFADNV 60

Query: 61  RFYAAPVQFQGKA-KEEKDTSGPRLNDQIKADFVRLVHDDG-HTILSLHEALERARDLKL 120
           RFYAAPVQFQGKA KEEKDTSGPRLND+IKAD VRLV+DDG HTILSL EALERAR+LKL
Sbjct: 61  RFYAAPVQFQGKAKKEEKDTSGPRLNDKIKADLVRLVYDDGQHTILSLREALERARELKL 120

Query: 121 DLVEVQQKANPPVCKIMDFHREKYKQQIREKDRVKSKENLLVVSEVSLKTGDHKEVRFTG 180
           DLVEVQQKANPPVCKIMDFHR+KYKQQI+EKDRVKSK      +EVSL+ GDHKEVRFTG
Sbjct: 121 DLVEVQQKANPPVCKIMDFHRQKYKQQIKEKDRVKSK------AEVSLRKGDHKEVRFTG 180

Query: 181 KTEEKDLRMKADMVKRLMERGYRVKCSAKGRENEDLGDLLSRLSALIEDVAVVESGPRVD 240
           KTEEKDLRMKADM+KRLMERGYRVKCSA+G EN+DLG LLSRLSALIEDVA+VESGPR++
Sbjct: 181 KTEEKDLRMKADMIKRLMERGYRVKCSARGGENQDLGGLLSRLSALIEDVAIVESGPRLE 240

Query: 241 KGQAYVIVRHVKFGPLKKGGGGKALKVVEKKKQKVQNGTTPLVSAARSSSSDEVEMGPEE 300
           KGQAYV+VRHVKFGP KKGGGGKA KVV   KQ VQNGTTPLVSAARSSS  + EM PEE
Sbjct: 241 KGQAYVVVRHVKFGPSKKGGGGKASKVVANAKQMVQNGTTPLVSAARSSSPCDKEMDPEE 300

Query: 301 SETEDDFESSKKTTWSFVDGNKDFDEVFDLKDDANGIPSTATTKKMNVVSDRDVNVSDLS 360
           S+ E+DFESS+KTTWS VD N D DEVFDLKDD NGIPST TTKKMNVVSD DV  S + 
Sbjct: 301 SDPENDFESSEKTTWSVVDRNNDLDEVFDLKDDTNGIPSTTTTKKMNVVSDGDVRTSSV- 360

Query: 361 KLRQLQDSPITADNRYKKTAPTNRFQPSNVADRNSRGARDPLRSEPENRDQRRHPPTNTN 420
            L Q QDS    DNRYKK  PTNRFQP NV DRNSRGARD LRSEP+ RDQRRHPPTNTN
Sbjct: 361 PLSQ-QDSSFKVDNRYKKIEPTNRFQPPNVMDRNSRGARDSLRSEPQIRDQRRHPPTNTN 420

Query: 421 FSPSMREARRYDNNTSASRNSNHSSNETSKPGPLQHTNPGPSKTSFGIFSSLNSNAPRKQ 480
           FSPSMRE  +YDNNTS+SRN  HSSNETSK GP  HTN GPSKTSFGIFSSLNSNAP KQ
Sbjct: 421 FSPSMRETSQYDNNTSSSRNVKHSSNETSKLGPSHHTNSGPSKTSFGIFSSLNSNAPGKQ 480

Query: 481 DAPATSYQGNRGPPYTPSANPNAPGAAGNTKFSITNSEDNLNKGQKSWGIFSKGSPNDNP 540
           D PATSYQ NRGPP+TPS NPNAPGAAGNTKF ITNSEDNLN+GQKSWG+FSKGSPND P
Sbjct: 481 DPPATSYQHNRGPPFTPSTNPNAPGAAGNTKFPITNSEDNLNEGQKSWGVFSKGSPNDTP 538

Query: 541 DRISKSN 546
           DRISKSN
Sbjct: 541 DRISKSN 538

BLAST of Lsi10G005050 vs. NCBI nr
Match: XP_022140534.1 (translation initiation factor IF3-1, mitochondrial [Momordica charantia])

HSP 1 Score: 745.0 bits (1922), Expect = 4.7e-211
Identity = 409/557 (73.43%), Postives = 447/557 (80.25%), Query Frame = 0

Query: 1   MTFFCRINQSKLKYLSDQFKRHYFQFSYPASLDSGRTTKHDSGYLGRIIPRRPADFADNV 60
           M FFCRINQSKLK LS+QFKRHYFQ SY +SL SGR  KH S  L R+IP RPAD    V
Sbjct: 1   MAFFCRINQSKLKLLSNQFKRHYFQISYASSLGSGRREKHVSENLERVIPIRPADLGGKV 60

Query: 61  RFYAAPVQFQGKA-KEEKDTSGPRLNDQIKADFVRLVHDDGHTILSLHEALERARDLKLD 120
           RFYAAPVQFQGKA KEEKDTSGPRLND+IKA+FVRLV DDGHTILSLHEALERAR+LKLD
Sbjct: 61  RFYAAPVQFQGKAKKEEKDTSGPRLNDKIKAEFVRLVTDDGHTILSLHEALERARELKLD 120

Query: 121 LVEVQQKANPPVCKIMDFHREKYKQQIREKDRVKSKENLLVVSEVSLKTGDHKEVRFTGK 180
           LVEVQQKANPPVCKIMDFHREKYKQQIREKDRVKSK      +EVSL+ GDHKEVRFTGK
Sbjct: 121 LVEVQQKANPPVCKIMDFHREKYKQQIREKDRVKSK------AEVSLRKGDHKEVRFTGK 180

Query: 181 TEEKDLRMKADMVKRLMERGYRVKCSAKGRENEDLGDLLSRLSALIEDVAVVESGPRVDK 240
           TEEKDLRMKADMVKRLMERGYRVKCSA+G E++DLG LLSRLSALIED AVVESGPRV++
Sbjct: 181 TEEKDLRMKADMVKRLMERGYRVKCSARGGEDQDLGGLLSRLSALIEDEAVVESGPRVER 240

Query: 241 GQAYVIVRHVKFGPLKKGGGGKALKVVEKKKQKVQNGTTPLVSAARSSSSDEVEMGPEES 300
           GQAYV+VRHVKFGP KKGGGGKA KVV   + K+QNGT PLVS AR+S + E EM PEES
Sbjct: 241 GQAYVVVRHVKFGPSKKGGGGKASKVVANPRLKIQNGTAPLVSVARASRTSEEEMDPEES 300

Query: 301 ETEDDFESSKKTTWSFVDGNKDFDEVFDLKDDANGIPSTATTKKMNVVSDRDVNVSDLSK 360
           ++E D E+SK+ TWS VDGN DFDEVFDLKDDAN IPS  T KKM+VVSDRDVN+SDLS+
Sbjct: 301 DSEADLENSKE-TWSAVDGNNDFDEVFDLKDDANRIPSKYTAKKMDVVSDRDVNISDLSQ 360

Query: 361 LRQL-------------QDSPITADNRYKKTAPTNRFQPSNVADRNSRGARDPLRSEPEN 420
           LR+L             QDS + A+NRYKK  P  RFQP+NV D   RGARD  RSE + 
Sbjct: 361 LRRLPDSGRTSSVPLSQQDSSVKAENRYKKIEPVTRFQPTNVMDNKGRGARDSHRSETQI 420

Query: 421 RDQRRHPPTNTNFSPSMREARRYDNNTSASRNSNHSSNETSKPGPLQHTNPGPSKTSFGI 480
           RDQRRH PTN NFSP MRE RR +NNTS  RN  H  N TSKP P  H +P PSKTSFGI
Sbjct: 421 RDQRRHRPTNPNFSPPMRETRREENNTSPLRNIKHPLNATSKPDPSHHADPSPSKTSFGI 480

Query: 481 FSSLNSNAPRKQDAPATSYQGNRGPPYTPSANPNAPGAAGNTKFSITNSEDNLNKGQKSW 540
           FSSLN+NAP KQDA ATSYQ NR PPY PS NP   GA+ NTKF  T S +N+NKG+KSW
Sbjct: 481 FSSLNANAPGKQDAAATSYQSNRCPPYAPSNNPYLHGASENTKFPATKSNENINKGKKSW 540

Query: 541 GIFSKGSPNDNPDRISK 544
           GIFSK S ND P+RISK
Sbjct: 541 GIFSKDSQNDPPNRISK 550

BLAST of Lsi10G005050 vs. NCBI nr
Match: XP_023534160.1 (translation initiation factor IF3-1, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 721.1 bits (1860), Expect = 7.3e-204
Identity = 402/560 (71.79%), Postives = 445/560 (79.46%), Query Frame = 0

Query: 1   MTFFCRINQSKLKYLSDQFKRHYFQFSYPASLDSGRTTKHDSGYLGRIIPRRPADFADNV 60
           M F CRINQSKL + S+QFKRHYF   Y +S+DSGRTTKH S Y+ RIIPR PADF   V
Sbjct: 1   MAFLCRINQSKLTHFSNQFKRHYFHICYASSIDSGRTTKHVSEYIARIIPRGPADFGGKV 60

Query: 61  RFYAAPVQFQGKA-KEEKDTSGPRLNDQIKADFVRLVHDDGHTILSLHEALERARDLKLD 120
           RFYAAPVQFQGKA KEE +TSGPRLND+IKA+FVRLV DDGH I+SL EAL RAR+LKLD
Sbjct: 61  RFYAAPVQFQGKAKKEENNTSGPRLNDKIKAEFVRLVSDDGHAIVSLREALGRARELKLD 120

Query: 121 LVEVQQKANPPVCKIMDFHREKYKQQIREKDRVKSKENLLVVSEVSLKTGDHKEVRFTGK 180
           LVEVQQKANPPVCKIMDFHREKYKQQIREKDRVKSK      +EV L+ GDHKEVRFTGK
Sbjct: 121 LVEVQQKANPPVCKIMDFHREKYKQQIREKDRVKSK------AEVGLRKGDHKEVRFTGK 180

Query: 181 TEEKDLRMKADMVKRLMERGYRVKCSAKGRENEDLGDLLSRLSALIEDVAVVESGPRVDK 240
           TEEKDLRMKADMVKRLMERGYRVKCSA+G E++DLG LLSRLSALIEDVA+VESGPRV++
Sbjct: 181 TEEKDLRMKADMVKRLMERGYRVKCSARGGEDQDLGGLLSRLSALIEDVAMVESGPRVER 240

Query: 241 GQAYVIVRHVKFGPLKKGGGGKALKVVEKKKQKVQNGTTPLVSAARSSSSDEVEMGPEES 300
           GQAYVIVRHVKFGP KKGGGGKA KV    +Q+V+NGTTP V AARSS+ DE EMGPEES
Sbjct: 241 GQAYVIVRHVKFGPSKKGGGGKASKVAVNARQEVKNGTTPPVPAARSSTPDEEEMGPEES 300

Query: 301 ETEDDFESSKKTTWSFVDGNKDFDEVFDLKDDANGIPSTATTKKMNVVSDRDVNVSDLSK 360
           ++E         TWS VDGN DFDEVFDLKDDANGIP   T  KMNV SDRDVNVSDLS 
Sbjct: 301 DSE--------ATWSVVDGNNDFDEVFDLKDDANGIPLETTANKMNVASDRDVNVSDLSP 360

Query: 361 LRQL-------------QDSPITADNRYKKTAPTNRFQPSNVADRNSRGARDPLRSEPEN 420
             +L             QDS   ADNRYKK  P NRFQP NV D   RGARD LRSEP+ 
Sbjct: 361 PSRLPGTSRTSSVPLSRQDSSTKADNRYKKIEPANRFQPPNVMDNKGRGARDSLRSEPQI 420

Query: 421 RDQRRHPPTNTNFS-PSMREARRYDNNTSASRNSNHSSNETSKPGPLQHTNPGPSKTSFG 480
           R+Q R P TNTN S PSMRE R  DN +SASRN+ + SNETSK G  QHT P PSKTSFG
Sbjct: 421 RNQWRQPLTNTNLSPPSMRETRPDDNVSSASRNTTYPSNETSKSGSSQHTVPDPSKTSFG 480

Query: 481 IFSSLNSNAPRKQDAPATSYQGNRGPPYTPSANPNAPGAAGNTKFSITNSEDNLNKGQKS 540
           IFS+LN+NAP KQDA +TSY+GNRG    PS NPNAPGA+ + KFS+T S+DN+NKGQKS
Sbjct: 481 IFSALNANAPVKQDAASTSYRGNRG----PSTNPNAPGASESLKFSLTKSDDNVNKGQKS 540

Query: 541 WGIFSKGSPNDNPDRISKSN 546
           WGIFS+G PN++P+RISK N
Sbjct: 541 WGIFSEG-PNNSPNRISKPN 541

BLAST of Lsi10G005050 vs. NCBI nr
Match: XP_022947975.1 (translation initiation factor IF3-1, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 715.7 bits (1846), Expect = 3.1e-202
Identity = 399/560 (71.25%), Postives = 442/560 (78.93%), Query Frame = 0

Query: 1   MTFFCRINQSKLKYLSDQFKRHYFQFSYPASLDSGRTTKHDSGYLGRIIPRRPADFADNV 60
           M FFCRINQSKL + S+QFKRHYFQ  Y  S+ SGR+TKHDS Y+ RIIPR PADF   V
Sbjct: 1   MAFFCRINQSKLTHFSNQFKRHYFQVCYAPSIGSGRSTKHDSEYIARIIPRGPADFGGKV 60

Query: 61  RFYAAPVQFQGKA-KEEKDTSGPRLNDQIKADFVRLVHDDGHTILSLHEALERARDLKLD 120
           RFYAAPVQFQGKA KEE +TSGPRLND+IKA+FVRLV DDGH I+SL EAL RAR+LKLD
Sbjct: 61  RFYAAPVQFQGKAKKEENNTSGPRLNDKIKAEFVRLVSDDGHAIVSLREALGRARELKLD 120

Query: 121 LVEVQQKANPPVCKIMDFHREKYKQQIREKDRVKSKENLLVVSEVSLKTGDHKEVRFTGK 180
           LVEVQQKANPPVCKIMDFHREKYKQQIREKDRVKSK      +E  L+ GDHKEVRFTGK
Sbjct: 121 LVEVQQKANPPVCKIMDFHREKYKQQIREKDRVKSK------AEGGLRKGDHKEVRFTGK 180

Query: 181 TEEKDLRMKADMVKRLMERGYRVKCSAKGRENEDLGDLLSRLSALIEDVAVVESGPRVDK 240
           TEEKDLRMK DMVKRLMERGYRVKCSA+G E++DLG LLSRLSALIEDVA+VESGPRV++
Sbjct: 181 TEEKDLRMKVDMVKRLMERGYRVKCSARGGEDQDLGGLLSRLSALIEDVAMVESGPRVER 240

Query: 241 GQAYVIVRHVKFGPLKKGGGGKALKVVEKKKQKVQNGTTPLVSAARSSSSDEVEMGPEES 300
           GQAYVIVRHVKFGP KKGGGGKA KV    +Q+V+NGTTPLV AARSS+ DE EMGPEES
Sbjct: 241 GQAYVIVRHVKFGPSKKGGGGKASKVAVNARQEVKNGTTPLVPAARSSNPDEEEMGPEES 300

Query: 301 ETEDDFESSKKTTWSFVDGNKDFDEVFDLKDDANGIPSTATTKKMNVVSDRDVNVSDLSK 360
           ++          TWS VDGN DFDEVFDLKDDA GIP   T  KMNV SDRDVNVSDLS 
Sbjct: 301 DS--------GATWSVVDGNNDFDEVFDLKDDAKGIPLETTANKMNVASDRDVNVSDLSP 360

Query: 361 LRQL-------------QDSPITADNRYKKTAPTNRFQPSNVADRNSRGARDPLRSEPEN 420
             +L             QDS   ADNRYKK  P NRFQP NV D   RGARD LRSEP+ 
Sbjct: 361 PTRLPDTSRTSSVPLSRQDSSTKADNRYKKIEPANRFQPPNVMDNKGRGARDSLRSEPQI 420

Query: 421 RDQRRHPPTNTNFS-PSMREARRYDNNTSASRNSNHSSNETSKPGPLQHTNPGPSKTSFG 480
           R+Q R P TNTN S PSMRE R  DN +SASRN+ H SNETSK G  QHT P PSKTSFG
Sbjct: 421 RNQWRQPLTNTNLSPPSMRETRPDDNVSSASRNTKHPSNETSKSGSSQHTVPDPSKTSFG 480

Query: 481 IFSSLNSNAPRKQDAPATSYQGNRGPPYTPSANPNAPGAAGNTKFSITNSEDNLNKGQKS 540
           IFS+LN+NAP KQDA +TSY+GNRG    PS NPNAPGA+ + KFS+T S++N+NKG KS
Sbjct: 481 IFSALNANAPVKQDAASTSYRGNRG----PSTNPNAPGASESLKFSLTKSDNNVNKGSKS 540

Query: 541 WGIFSKGSPNDNPDRISKSN 546
           WGIFS+G PN++P+RISK N
Sbjct: 541 WGIFSEG-PNNSPNRISKPN 541

BLAST of Lsi10G005050 vs. NCBI nr
Match: XP_022970983.1 (translation initiation factor IF3-1, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 703.0 bits (1813), Expect = 2.0e-198
Identity = 392/559 (70.13%), Postives = 432/559 (77.28%), Query Frame = 0

Query: 1   MTFFCRINQSKLKYLSDQFKRHYFQFSYPASLDSGRTTKHDSGYLGRIIPRRPADFADNV 60
           M FFCRIN         QFKRHYFQ  Y +S+ SGRTTKH S Y+ RIIPR PADF   V
Sbjct: 1   MAFFCRIN---------QFKRHYFQVCYASSIGSGRTTKHVSEYISRIIPRGPADFGGKV 60

Query: 61  RFYAAPVQFQGKA-KEEKDTSGPRLNDQIKADFVRLVHDDGHTILSLHEALERARDLKLD 120
           RFYAAPVQFQGKA KEE +TSGPRLND+IKA+FVRLV DDGH ILSL EAL RAR+LKLD
Sbjct: 61  RFYAAPVQFQGKAKKEENNTSGPRLNDKIKAEFVRLVSDDGHAILSLREALGRARELKLD 120

Query: 121 LVEVQQKANPPVCKIMDFHREKYKQQIREKDRVKSKENLLVVSEVSLKTGDHKEVRFTGK 180
           LVEVQQKANPPVCKIMDFHREKYKQQIREKDRVKSK      +EV L+ GDHKEVRFTGK
Sbjct: 121 LVEVQQKANPPVCKIMDFHREKYKQQIREKDRVKSK------AEVGLRKGDHKEVRFTGK 180

Query: 181 TEEKDLRMKADMVKRLMERGYRVKCSAKGRENEDLGDLLSRLSALIEDVAVVESGPRVDK 240
           TEEKDLRMKADMVKRLME+GYRVKCSA+G E++DLG LLSRLSALIEDVA+VESGPRV++
Sbjct: 181 TEEKDLRMKADMVKRLMEQGYRVKCSARGGEDQDLGGLLSRLSALIEDVAMVESGPRVER 240

Query: 241 GQAYVIVRHVKFGPLKKGGGGKALKVVEKKKQKVQNGTTPLVSAARSSSSDEVEMGPEES 300
           GQAYVIVRHVKFGP KKGGGGKA KV    +Q+V NGTTPLV AARSS+ DE E GPEE 
Sbjct: 241 GQAYVIVRHVKFGPSKKGGGGKASKVAVSARQEVINGTTPLVPAARSSTPDEEEKGPEEP 300

Query: 301 ETEDDFESSKKTTWSFVDGNKDFDEVFDLKDDANGIPSTATTKKMNVVSDRDVNVSDLSK 360
           ++E         TWS VDGN DFDEVFDLKDDANGIP   T  KMNV SDRD NVSDLS 
Sbjct: 301 DSE--------ATWSVVDGNNDFDEVFDLKDDANGIPLETTANKMNVASDRDANVSDLSP 360

Query: 361 LRQL-------------QDSPITADNRYKKTAPTNRFQPSNVADRNSRGARDPLRSEPEN 420
             +L             QDS   ADNRYKK  P NRFQP NV D   RGARD LRS+P+ 
Sbjct: 361 PTRLPDTSRTSSVPLSRQDSSTKADNRYKKIEPANRFQPPNVTDNKGRGARDSLRSDPQI 420

Query: 421 RDQRRHPPTNTNFSPSMREARRYDNNTSASRNSNHSSNETSKPGPLQHTNPGPSKTSFGI 480
           R+QRR         P M+E R  DNN+SASRN  H SNETSK GP  HT+PGPSKTSFGI
Sbjct: 421 RNQRR--------QPLMKETRLDDNNSSASRNIKHPSNETSKSGPSHHTDPGPSKTSFGI 480

Query: 481 FSSLNSNAPRKQDAPATSYQGNRGPPYTPSANPNAPGAAGNTKFSITNSEDNLNKGQKSW 540
           FSSLN+NAP KQDA +TSY+GNRG PY PS NPN PGA+ + KFS+T S+DN+NKG KSW
Sbjct: 481 FSSLNANAPGKQDAASTSYRGNRGLPYPPSTNPNVPGASESLKFSLTKSDDNVNKGSKSW 527

Query: 541 GIFSKGSPNDNPDRISKSN 546
           GIFS+G PN++P+RISK N
Sbjct: 541 GIFSEG-PNNSPNRISKPN 527

BLAST of Lsi10G005050 vs. TAIR 10
Match: AT1G34360.1 (translation initiation factor 3 (IF-3) family protein )

HSP 1 Score: 173.3 bits (438), Expect = 5.3e-43
Identity = 170/515 (33.01%), Postives = 250/515 (48.54%), Query Frame = 0

Query: 7   INQSKLKYLSDQFKRHYFQFSYPASLDS--GRTTKHDSGYL-GRIIPRRPADFADNVRFY 66
           IN+S LKY S+Q  R+Y+     AS      +TTK  S  +    I  RP++   N+RF 
Sbjct: 7   INRSYLKYASNQLTRNYYTQVCLASSTHVVKQTTKLSSFDIPNSDICTRPSNIFQNLRFL 66

Query: 67  AAPVQFQGKAKEEKDTSGPRLNDQIKADFVRLVHDDGHTILSLHEALERARDLKLDLVEV 126
           A   Q + K + E D+ GPRLN++I  D+VRLV ++GH ++SL EAL RA++L+ DLVEV
Sbjct: 67  ATSAQTR-KKEAEVDSDGPRLNEKITGDYVRLVSEEGHCVVSLREALRRAKELQCDLVEV 126

Query: 127 QQKANPPVCKIMDFHREKYKQQIREKDRVKSKENLLVVSEVSLKTGDHKEVRFTGKTEEK 186
           Q+ A PPVCKI+ +  E YK+    K+R K+K    +  ++       KE+RFT K E K
Sbjct: 127 QRDAKPPVCKIVKYSLELYKKAKVGKERAKAKRAEAIRPDI-------KEIRFTPKIEAK 186

Query: 187 DLRMKADMVKRLMERGYRVKCSA-------KGRENEDLGDLLSRLSALIEDVAVVESGPR 246
           DL+ K+D   +LME GYRVKC A       K  E E L +LL R +  I D A+VES P 
Sbjct: 187 DLKFKSDQALKLMESGYRVKCLAVPDKDKHKELEPEKLLELLFRFTCFIGD-ALVESWPE 246

Query: 247 VDKGQAYVIVRHVKFGPLKKGGGGKALKVVEKKKQKVQNGTTPLVSAARSSSSDEVEMGP 306
            D+  A VIVRH KFGP KK GG K +K ++ K  +V+  +    S+    ++ + +   
Sbjct: 247 ADRKGAVVIVRHAKFGPPKK-GGVKLMKDIDIKSARVKEESPKPDSSKAGVATVDDQEDI 306

Query: 307 EESETEDDFESSKKTTWSFVDGNKDFDEVFDLKDDANGIPSTATTKKMNVVSDRDVNVSD 366
           E+SE     E ++   +      ++    F    DA+     +  + +N    R  N + 
Sbjct: 307 EKSEPRFSVEQAQPVKFQNAYAKREPSSEFSGGRDASRFEPQSPNQHVNPQRPRFSNQAP 366

Query: 367 LSKLR-----QLQDSPITADN-RYKKTAPTNRFQPSNVADRNSRGARDPLRSE-PENRDQ 426
             +       Q  + P +A   ++    PT RF P       S+  R    ++ P  +  
Sbjct: 367 NQQPTGRFDPQSPNQPPSAPRPQFPNQQPTGRFDP----QFPSQPPRPQFPNQAPNQQST 426

Query: 427 RRHPPTNTNFSPSMREARRYDNNTSASRNSNHSSNETSKPG-PLQHTNPGPSKTSFGIFS 486
            R  P   N  PS  ++R  D   +   +    +    + G P +  N  P++   G F 
Sbjct: 427 GRFNPQFPNQRPSPPQSRFPDQAPNQQPSGPSPNRHPDRQGPPPRFQNQAPNQQPTGRFE 486

Query: 487 SLNSNAPRKQDAPATSYQGNRGPPYTPSANPNAPG 504
               N PR    P T     R P  T +  P APG
Sbjct: 487 PQPPNPPRAPPRPQT-----RLPNETSNEQPTAPG 502

BLAST of Lsi10G005050 vs. TAIR 10
Match: AT4G30690.1 (Translation initiation factor 3 protein )

HSP 1 Score: 65.1 bits (157), Expect = 2.0e-10
Identity = 65/234 (27.78%), Postives = 114/234 (48.72%), Query Frame = 0

Query: 66  PVQFQGKAKEEKDTSGPRLNDQIKADFVRLVHDDGHTI--LSLHEALERARDLKLDLVEV 125
           P   +G+ KE +D     ++  I++  VRL+ D  + I  +S  EA+ RA D +LDLV +
Sbjct: 70  PGDRRGRQKESEDDDSLDIS-AIRSATVRLIDDQQNMIGLVSKEEAVRRAEDAELDLVIL 129

Query: 126 QQKANPPVCKIMDFHREKYKQQIREKDRVKSKENLLVVSEVSLKTGDHKEVRFTGKTEEK 185
              A+PPV ++MD+ + +Y+QQ R+K++ K    +           D KE++     ++ 
Sbjct: 130 SPDADPPVVRMMDYSKYRYEQQKRKKEQQKKTTRM-----------DLKELKMGYNIDQH 189

Query: 186 DLRMKADMVKRLMERGYRVK--CSAKGRENEDLG---DLLSRLSALIEDVAVVESGPRVD 245
           D  ++    ++ ++ G +VK   + KGRENE      +LL R    I ++   ES    D
Sbjct: 190 DYSVRMRAARKFLQDGDKVKVIVNMKGRENEFRNIAIELLRRFQTEIGELGTEESKNFRD 249

Query: 246 KGQAYVIVRHVKFGPLKKGGGGKALKVVEKKKQKVQNGTTPLVSAARSSSSDEV 293
           +    V+V      P K+       KV E   +K +      VSAA  +++ ++
Sbjct: 250 RNLFIVLV------PNKE----VIRKVQEPPPKKKKKPADDKVSAANITATQDI 281

BLAST of Lsi10G005050 vs. TAIR 10
Match: AT2G24060.1 (Translation initiation factor 3 protein )

HSP 1 Score: 59.3 bits (142), Expect = 1.1e-08
Identity = 67/227 (29.52%), Postives = 109/227 (48.02%), Query Frame = 0

Query: 88  IKADFVRLVHDDGHT--ILSLHEALERARDLKLDLVEVQQKANPPVCKIMDFHREKYKQQ 147
           I++  VRL+    +   ++S  EA+  A D +LDLV +   A+PPV K+MD+ + +Y+QQ
Sbjct: 95  IRSATVRLIDGQQNMLGLVSKDEAVRMADDAELDLVILSPDADPPVVKMMDYSKYRYEQQ 154

Query: 148 IREKDRVKSKENLLVVSEVSLKTGDHKEVRFTGKTEEKDLRMKADMVKRLMERGYRVK-- 207
            R+KD+ K    +           D KE++     ++ D  ++    ++ ++ G +VK  
Sbjct: 155 KRKKDQQKKTTRM-----------DLKELKMGYNIDQHDYSVRLRAAQKFLQDGDKVKVI 214

Query: 208 CSAKGRENEDLG---DLLSRLSALIEDVAVVESGPRVDKGQAYVIVRHVKFGPLKKGGGG 267
            S KGRENE      +LL R    I ++A  ES    D+    ++V      P K+    
Sbjct: 215 VSMKGRENEFRNIAIELLRRFQTEIGELATEESKNFRDRNMFIILV------PNKEMIRK 274

Query: 268 KALKVVEKKKQKVQNGTTPLVSAARSSSSDEVEMGPE-ESETEDDFE 307
                  KKK+  +N  +   SAA  ++  E E  PE E E E + E
Sbjct: 275 PQEPPTRKKKKTAENEAS--ASAAEITAEPEPEPEPEPEPEPEPEPE 302

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6NLP27.4e-4233.01Translation initiation factor IF3-1, mitochondrial OS=Arabidopsis thaliana OX=37... [more]
Q5LQ586.7e-1934.92Translation initiation factor IF-3 OS=Ruegeria pomeroyi (strain ATCC 700808 / DS... [more]
Q8PJE26.3e-1734.09Translation initiation factor IF-3 OS=Xanthomonas axonopodis pv. citri (strain 3... [more]
Q5QYN51.8e-1631.89Translation initiation factor IF-3 OS=Idiomarina loihiensis (strain ATCC BAA-735... [more]
Q8P7Z31.8e-1633.52Translation initiation factor IF-3 OS=Xanthomonas campestris pv. campestris (str... [more]
Match NameE-valueIdentityDescription
A0A6J1CFY92.3e-21173.43translation initiation factor IF3-1, mitochondrial OS=Momordica charantia OX=367... [more]
A0A6J1G8E41.5e-20271.25translation initiation factor IF3-1, mitochondrial-like OS=Cucurbita moschata OX... [more]
A0A6J1I5H49.9e-19970.13translation initiation factor IF3-1, mitochondrial-like OS=Cucurbita maxima OX=3... [more]
A0A0A0LJ901.4e-18965.70IF3_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G270880 PE=3 ... [more]
A0A6J1FYA85.5e-18970.09translation initiation factor IF3-1, mitochondrial-like OS=Cucurbita moschata OX... [more]
Match NameE-valueIdentityDescription
XP_038902038.13.2e-23681.90translation initiation factor IF3-1, mitochondrial [Benincasa hispida][more]
XP_022140534.14.7e-21173.43translation initiation factor IF3-1, mitochondrial [Momordica charantia][more]
XP_023534160.17.3e-20471.79translation initiation factor IF3-1, mitochondrial-like [Cucurbita pepo subsp. p... [more]
XP_022947975.13.1e-20271.25translation initiation factor IF3-1, mitochondrial-like [Cucurbita moschata][more]
XP_022970983.12.0e-19870.13translation initiation factor IF3-1, mitochondrial-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT1G34360.15.3e-4333.01translation initiation factor 3 (IF-3) family protein [more]
AT4G30690.12.0e-1027.78Translation initiation factor 3 protein [more]
AT2G24060.11.1e-0829.52Translation initiation factor 3 protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 106..126
NoneNo IPR availableCOILSCoilCoilcoord: 135..155
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 363..391
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 413..545
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 363..545
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 394..412
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 272..289
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 267..310
NoneNo IPR availablePANTHERPTHR10938:SF4TRANSLATION INITIATION FACTOR IF3-1, MITOCHONDRIALcoord: 1..532
IPR001288Translation initiation factor 3TIGRFAMTIGR00168TIGR00168coord: 84..245
e-value: 2.0E-26
score: 90.6
IPR001288Translation initiation factor 3PANTHERPTHR10938TRANSLATION INITIATION FACTOR IF-3coord: 1..532
IPR036788Translation initiation factor 3 (IF-3), C-terminal domain superfamilyGENE3D3.30.110.10coord: 164..251
e-value: 4.1E-13
score: 51.0
IPR036788Translation initiation factor 3 (IF-3), C-terminal domain superfamilySUPERFAMILY55200Translation initiation factor IF3, C-terminal domaincoord: 171..250
IPR019814Translation initiation factor 3, N-terminalPFAMPF05198IF3_Ncoord: 84..150
e-value: 1.6E-23
score: 82.8
IPR036787Translation initiation factor 3 (IF-3), N-terminal domain superfamilyGENE3D3.10.20.80coord: 75..150
e-value: 1.6E-25
score: 90.9
IPR036787Translation initiation factor 3 (IF-3), N-terminal domain superfamilySUPERFAMILY54364Translation initiation factor IF3, N-terminal domaincoord: 81..151

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi10G005050.1Lsi10G005050.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032790 ribosome disassembly
biological_process GO:0006413 translational initiation
molecular_function GO:0043022 ribosome binding
molecular_function GO:0003743 translation initiation factor activity