Lsi10G003690 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi10G003690 vs. ExPASy Swiss-Prot
Match: Q8IUS5 (Epoxide hydrolase 4 OS=Homo sapiens OX=9606 GN=EPHX4 PE=1 SV=2)

HSP 1 Score: 58.2 bits (139), Expect = 1.7e-07
Identity = 47/159 (29.56%), Postives = 73/159 (45.91%), Query Frame = 0

Query: 44  VQIEPGTTMNFWVPAGNPDKNHTNHPPLLLLHGFAANAIMTWQFQVLNLAKNYAVYVPDF 103
           V+I+       +V AG   K     P +LLLHGF      +W++Q+      Y V   D 
Sbjct: 74  VRIKDSGLRFHYVAAGERGK-----PLMLLLHGF-PEFWYSWRYQLREFKSEYRVVALDL 133

Query: 104 IFFGESVTDRPDRSPEFQAKC----VAEGLRKLGVDRRCVLVGFSYGVMVGFRLAGMYPE 163
             +GE  TD P     ++  C    + + L  LG   +CVL+G  +G M+ + +A  YPE
Sbjct: 134 RGYGE--TDAPIHRQNYKLDCLITDIKDILDSLGYS-KCVLIGHDWGGMIAWLIAICYPE 193

Query: 164 LVEAVVVT--AAPPVLTELISREALEKIGFKSWSEYLIP 197
           +V  ++V     P V TE I R   + +    +  + IP
Sbjct: 194 MVMKLIVINFPHPNVFTEYILRHPAQLLKSSYYYFFQIP 223

BLAST of Lsi10G003690 vs. ExPASy Swiss-Prot
Match: Q6IE26 (Epoxide hydrolase 4 OS=Mus musculus OX=10090 GN=Ephx4 PE=2 SV=2)

HSP 1 Score: 57.0 bits (136), Expect = 3.8e-07
Identity = 46/160 (28.75%), Postives = 72/160 (45.00%), Query Frame = 0

Query: 44  VQIEPGTTMNFWVPAGNPDKNHTNHPPLLLLHGFAANAIMTWQFQVLNLAKNYAVYVPDF 103
           V+I+       +V AG   K     P +LLLHGF      +W+ Q+      Y V   D 
Sbjct: 72  VRIKDSGLRFHYVAAGERGK-----PLMLLLHGF-PEFWYSWRHQLREFKSEYRVVALDL 131

Query: 104 IFFGESVTDRPDRSPEFQAKC----VAEGLRKLGVDRRCVLVGFSYGVMVGFRLAGMYPE 163
             +GES  D P     ++  C    + + L  LG   +CVL+G  +G M+ + +A  YPE
Sbjct: 132 RGYGES--DAPAHQESYKLDCLIADIKDILDSLGYS-KCVLIGHDWGGMIAWLIAVCYPE 191

Query: 164 LVEAVVVT--AAPPVLTELISREALEKIGFKSWSEYLIPK 198
           ++  ++V     P V TE I R   +      +  + IP+
Sbjct: 192 MIMKLIVINFPHPSVFTEYILRHPAQLFRSSFYYFFQIPR 222

BLAST of Lsi10G003690 vs. ExPASy Swiss-Prot
Match: Q7SHI0 (Epoxide hydrolase srdG OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=srdG PE=2 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 3.2e-06
Identity = 42/143 (29.37%), Postives = 72/143 (50.35%), Query Frame = 0

Query: 48  PGTTMNFWVPAGNPDKNHTNHP--PLLLLHGFAANAIMTWQFQV-LNLAKNYAVYVPDFI 107
           P T++ +     NPD     HP   +LLLHG+  +  + W++QV   L+    V +PD +
Sbjct: 21  PSTSLTYHYLLANPDSASFPHPTATVLLLHGW-PDLSLGWRYQVPFLLSLGLRVIIPDML 80

Query: 108 FFGESVTDRPDRSPEFQAKCVA----EGLRKLGVDRRCVLVG-FSYGVMVGFRLAGMYPE 167
            +G  +T  P    E+  K ++      +R++    + VL+G   +G  +G+RLA  YPE
Sbjct: 81  GYG--LTSAPSSPSEYSLKNLSFHMTHIIRQVNPSGQPVLLGTHDWGAFLGWRLALYYPE 140

Query: 168 LVEAVVVTAAP--PVLTELISRE 181
           L++ +     P  P  T++ S E
Sbjct: 141 LIKGIFAFCIPYSPPETKVTSLE 160

BLAST of Lsi10G003690 vs. ExPASy Swiss-Prot
Match: Q3V1F8 (Epoxide hydrolase 3 OS=Mus musculus OX=10090 GN=Ephx3 PE=2 SV=2)

HSP 1 Score: 52.0 bits (123), Expect = 1.2e-05
Identity = 37/133 (27.82%), Postives = 63/133 (47.37%), Query Frame = 0

Query: 65  HTNHPPLLLLHGFAANAIMTWQFQVLNLAKNYAVYVPDFIFFGESVTDRPDRSPEFQAKC 124
           H N P +L LHGF  N   +W++Q+     ++ V   D    G S +D P     +    
Sbjct: 101 HGNGPLMLFLHGFPEN-WFSWRYQLREFQSHFHVVAVD--MRGYSPSDAPKEVDCYTIDL 160

Query: 125 VAEGLRK--LGVD-RRCVLVGFSYGVMVGFRLAGMYPELVEAVVVTAAPPVLTELISREA 184
           + + ++   LG+   +C+LV   +G  + +  +  YP LVE +VV   PP+   +I   +
Sbjct: 161 LLDDIKDTILGLGYSKCILVSHDWGASLAWEFSIYYPSLVERMVVANGPPM--SVIQEYS 220

Query: 185 LEKIGFKSWSEYL 195
           +  IG    S Y+
Sbjct: 221 IHHIGQIFRSNYM 228

BLAST of Lsi10G003690 vs. ExPASy Swiss-Prot
Match: A8GCT3 (Putative aminoacrylate hydrolase RutD OS=Serratia proteamaculans (strain 568) OX=399741 GN=rutD PE=3 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 6.1e-05
Identity = 39/105 (37.14%), Postives = 51/105 (48.57%), Query Frame = 0

Query: 70  PLLLLHGFAANAIMTWQFQVLNLAKNYAVYVPDFIFFGESVTDRPD-RSPEFQAKCVAEG 129
           P L+L      A   WQ Q+  L +++ V V D      S    PD  S    A  VA+ 
Sbjct: 14  PTLVLSAGLGGAGSFWQPQINALGEHFRVVVYDHFGTARSKGSVPDGYSMADMADEVAQL 73

Query: 130 LRKLGVDRRCVLVGFSYGVMVGFRLAGMYPELVEAVVVTAAPPVL 174
           LR L VD  C  VG + G M+G +LA  +P+LVE +VV    P L
Sbjct: 74  LRSLNVD-CCYFVGHALGGMIGLQLALTHPQLVEKLVVVNGWPTL 117

BLAST of Lsi10G003690 vs. ExPASy TrEMBL
Match: A0A0A0LLY6 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G247580 PE=4 SV=1)

HSP 1 Score: 423.7 bits (1088), Expect = 5.8e-115
Identity = 200/260 (76.92%), Postives = 228/260 (87.69%), Query Frame = 0

Query: 19  MVNIFHVYDRVLRWVMKAAGVRPVQVQIEPGTTMNFWVPAGNPDKNHTNHPPLLLLHGFA 78
           MVNIFH+YD+VLRWVMKAAG++PVQ+QI+PGT MNFWVPAG P+KNH NHPPLL LHGFA
Sbjct: 1   MVNIFHIYDQVLRWVMKAAGIKPVQLQIKPGTAMNFWVPAGKPNKNHQNHPPLLFLHGFA 60

Query: 79  ANAIMTWQFQVLNLAKNYAVYVPDFIFFGESVTDRPDRSPEFQAKCVAEGLRKLGVDRRC 138
            NAIMTWQFQVL  AKN+AVYVPDF+FFG+SVTDRPDR+ EFQA+CV EGLRKLGVDRR 
Sbjct: 61  TNAIMTWQFQVLKFAKNHAVYVPDFMFFGDSVTDRPDRTTEFQAECVVEGLRKLGVDRRF 120

Query: 139 VLVGFSYGVMVGFRLAGMYPELVEAVVVTAAPPVLTELISREALEKIGFKSWSEYLIPKT 198
           VLVGFSYG MVGFRLA MYPE+VEA+VVTAAP VLTE I+ EA+EKIG+KSWSEYLIP+T
Sbjct: 121 VLVGFSYGAMVGFRLAEMYPEMVEAMVVTAAPTVLTERITGEAMEKIGYKSWSEYLIPET 180

Query: 199 AKGVKTLLEIASFKFPRVPRWIFKHYLEAMVIHKTERKELLQALVAADD--------RLH 258
            KG  ++L+IASF+FPR PRWIFK YLEAMV+H+ ER ELL+ALVA +D        +LH
Sbjct: 181 VKGAISMLQIASFEFPRFPRWIFKQYLEAMVVHRKERAELLEALVAPNDVTISQYPQKLH 240

Query: 259 IIWGRNDNLFDVQIAYKMKE 271
           IIWGRNDNLFD+QIAY MKE
Sbjct: 241 IIWGRNDNLFDIQIAYNMKE 260

BLAST of Lsi10G003690 vs. ExPASy TrEMBL
Match: A0A1S4E310 (uncharacterized protein LOC103499739 OS=Cucumis melo OX=3656 GN=LOC103499739 PE=4 SV=1)

HSP 1 Score: 423.7 bits (1088), Expect = 5.8e-115
Identity = 202/260 (77.69%), Postives = 226/260 (86.92%), Query Frame = 0

Query: 19  MVNIFHVYDRVLRWVMKAAGVRPVQVQIEPGTTMNFWVPAGNPDKNHTNHPPLLLLHGFA 78
           ++NIFH+YD VLRWVMKAAG+ PVQ+QIEPGT+MNFWVPA  P+KNH NHPPLL LHGFA
Sbjct: 2   VINIFHIYDLVLRWVMKAAGIIPVQLQIEPGTSMNFWVPARKPNKNHQNHPPLLFLHGFA 61

Query: 79  ANAIMTWQFQVLNLAKNYAVYVPDFIFFGESVTDRPDRSPEFQAKCVAEGLRKLGVDRRC 138
           ANAIMTWQFQVL  AK +AVYVPDF+FFG+SVTDRPDRSPEFQA+CVAEGLRKLGV+RR 
Sbjct: 62  ANAIMTWQFQVLKFAKTHAVYVPDFMFFGDSVTDRPDRSPEFQAECVAEGLRKLGVERRF 121

Query: 139 VLVGFSYGVMVGFRLAGMYPELVEAVVVTAAPPVLTELISREALEKIGFKSWSEYLIPKT 198
           VLVGFSYG MVGFRLA  YPELVEA+VVTAAP VLTE I+  ALEKIG+KSWSEYLIP+T
Sbjct: 122 VLVGFSYGAMVGFRLAEKYPELVEAMVVTAAPTVLTERITEAALEKIGYKSWSEYLIPET 181

Query: 199 AKGVKTLLEIASFKFPRVPRWIFKHYLEAMVIHKTERKELLQALVAADD--------RLH 258
            KGVK++LEIASF+FPR PRWIFKHYLE M+IH  ER ELL+ALV  +D        +LH
Sbjct: 182 VKGVKSMLEIASFEFPRFPRWIFKHYLEGMIIHNKERAELLEALVTPNDVTISRYPQKLH 241

Query: 259 IIWGRNDNLFDVQIAYKMKE 271
           IIWGRNDNLFD+QIAY MKE
Sbjct: 242 IIWGRNDNLFDIQIAYNMKE 261

BLAST of Lsi10G003690 vs. ExPASy TrEMBL
Match: A0A5A7TQB4 (Epoxide hydrolase 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold303G00590 PE=4 SV=1)

HSP 1 Score: 384.8 bits (987), Expect = 3.0e-103
Identity = 182/229 (79.48%), Postives = 204/229 (89.08%), Query Frame = 0

Query: 19  MVNIFHVYDRVLRWVMKAAGVRPVQVQIEPGTTMNFWVPAGNPDKNHTNHPPLLLLHGFA 78
           ++NIFH+YD VLRWVMKAAG+ PVQ+QIEPGT+MNFWVPA  P+KNH NHPPLL LHGFA
Sbjct: 2   VINIFHIYDLVLRWVMKAAGIIPVQLQIEPGTSMNFWVPARKPNKNHQNHPPLLFLHGFA 61

Query: 79  ANAIMTWQFQVLNLAKNYAVYVPDFIFFGESVTDRPDRSPEFQAKCVAEGLRKLGVDRRC 138
           ANAIMTWQFQVL  AK +AVYVPDF+FFG+SVTDRPDRSPEFQA+CVAEGLRKLGV+RR 
Sbjct: 62  ANAIMTWQFQVLKFAKTHAVYVPDFMFFGDSVTDRPDRSPEFQAECVAEGLRKLGVERRF 121

Query: 139 VLVGFSYGVMVGFRLAGMYPELVEAVVVTAAPPVLTELISREALEKIGFKSWSEYLIPKT 198
           VLVGFSYG MVGFRLA  YPELVEA+VVTAAP VLTE I+  ALEKIG+KSWSEYLIP+T
Sbjct: 122 VLVGFSYGAMVGFRLAEKYPELVEAMVVTAAPTVLTERITEAALEKIGYKSWSEYLIPET 181

Query: 199 AKGVKTLLEIASFKFPRVPRWIFKHYLEAMVIHKTERKELLQALVAADD 248
            KGVK++LEIASF+FPR PRWIFKHYLE M+IH  ER ELL+ALV  +D
Sbjct: 182 VKGVKSMLEIASFEFPRFPRWIFKHYLEGMIIHNKERAELLEALVTPND 230

BLAST of Lsi10G003690 vs. ExPASy TrEMBL
Match: A0A6J1G806 (epoxide hydrolase 4-like OS=Cucurbita moschata OX=3662 GN=LOC111451675 PE=4 SV=1)

HSP 1 Score: 375.6 bits (963), Expect = 1.8e-100
Identity = 183/260 (70.38%), Postives = 211/260 (81.15%), Query Frame = 0

Query: 19  MVNIFHVYDRVLRWVMKAAGVRPVQVQIEPGTTMNFWVPAGNPDKNHTNHPPLLLLHGFA 78
           M NIFHVY RVLRW+MK AGVR   VQIEPGTTMNFWVP  NP KN   +PP++LLHGFA
Sbjct: 1   MANIFHVYHRVLRWLMKVAGVRQELVQIEPGTTMNFWVPTRNPKKN--RNPPVVLLHGFA 60

Query: 79  ANAIMTWQFQVLNLAKNYAVYVPDFIFFGESVTDRPDRSPEFQAKCVAEGLRKLGVDRRC 138
            N IMTWQFQV+NLA+NYAVYVPDF+FFGESVTDR +RSPEFQA+ V +GLRKLGV+R C
Sbjct: 61  GNGIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAEGVVKGLRKLGVERPC 120

Query: 139 VLVGFSYGVMVGFRLAGMYPELVEAVVVTAAPPVLTELISREALEKIGFKSWSEYLIPKT 198
           VLVGFSYG MVGFRLA MYP+LV A+VVT+AP  LTE +SR AL +IG+KSWS+YLIP T
Sbjct: 121 VLVGFSYGAMVGFRLAEMYPDLVAAMVVTSAPTALTESLSRVALLEIGYKSWSDYLIPNT 180

Query: 199 AKGVKTLLEIASFKFPRVPRWIFKHYLEAMVIHKTERKELLQALVAADD--------RLH 258
             GVK+L EI SF FP +P WI KHYLEAMV ++ E+ ELL+ALV  DD        ++H
Sbjct: 181 VNGVKSLFEIGSFDFPALPNWIMKHYLEAMVKYRKEQAELLRALVVPDDFTISQFPQKMH 240

Query: 259 IIWGRNDNLFDVQIAYKMKE 271
           IIWGRNDNLF++QIAY MKE
Sbjct: 241 IIWGRNDNLFNMQIAYNMKE 258

BLAST of Lsi10G003690 vs. ExPASy TrEMBL
Match: A0A6J1KWX2 (epoxide hydrolase 4-like OS=Cucurbita maxima OX=3661 GN=LOC111499509 PE=4 SV=1)

HSP 1 Score: 320.9 bits (821), Expect = 5.3e-84
Identity = 151/208 (72.60%), Postives = 177/208 (85.10%), Query Frame = 0

Query: 19  MVNIFHVYDRVLRWVMKAAGVRPVQVQIEPGTTMNFWVPAGNPDKNHTNHPPLLLLHGFA 78
           M NIFHVY RVLRW+MK AGVR   VQI+PGTTMNFWVP  NP KN   +PP++LLHGFA
Sbjct: 1   MANIFHVYHRVLRWLMKVAGVRQELVQIQPGTTMNFWVPTRNPKKN--PNPPVVLLHGFA 60

Query: 79  ANAIMTWQFQVLNLAKNYAVYVPDFIFFGESVTDRPDRSPEFQAKCVAEGLRKLGVDRRC 138
            N IMTWQFQV+NLA+NYAVYVPDF+FFGESVTDR +RSPEFQA+CV +GLR+LGV+R C
Sbjct: 61  GNGIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAECVVKGLRRLGVERPC 120

Query: 139 VLVGFSYGVMVGFRLAGMYPELVEAVVVTAAPPVLTELISREALEKIGFKSWSEYLIPKT 198
           VLVGFSYG MVGFRLA MYP+LV A+VVT+AP VLTE +SREAL++IG+KSWS+YLIP T
Sbjct: 121 VLVGFSYGAMVGFRLAEMYPDLVAAMVVTSAPTVLTESLSREALQEIGYKSWSDYLIPNT 180

Query: 199 AKGVKTLLEIASFKFPRVPRWIFKHYLE 227
            KGVK+L EI SF FP +P WI + Y++
Sbjct: 181 VKGVKSLFEIGSFDFPALPNWIMEQYVK 206

BLAST of Lsi10G003690 vs. NCBI nr
Match: XP_038902558.1 (epoxide hydrolase 4-like [Benincasa hispida])

HSP 1 Score: 454.9 bits (1169), Expect = 4.8e-124
Identity = 221/260 (85.00%), Postives = 238/260 (91.54%), Query Frame = 0

Query: 19  MVNIFHVYDRVLRWVMKAAGVRPVQVQIEPGTTMNFWVPAGNPDKNHTNHPPLLLLHGFA 78
           MVNIFH+YDRV+RWVMKAAGVRPVQVQI+PGTTMNFWVPAGNP+K H NHPPLLLLHGFA
Sbjct: 1   MVNIFHIYDRVVRWVMKAAGVRPVQVQIKPGTTMNFWVPAGNPNKIHQNHPPLLLLHGFA 60

Query: 79  ANAIMTWQFQVLNLAKNYAVYVPDFIFFGESVTDRPDRSPEFQAKCVAEGLRKLGVDRRC 138
           ANAIMTWQFQVLNLAK YAVYVPDFIFFGESVTDRPDRSPEFQA+CVAEGLRKLGV+R C
Sbjct: 61  ANAIMTWQFQVLNLAKKYAVYVPDFIFFGESVTDRPDRSPEFQAECVAEGLRKLGVERGC 120

Query: 139 VLVGFSYGVMVGFRLAGMYPELVEAVVVTAAPPVLTELISREALEKIGFKSWSEYLIPKT 198
           VLVGFSYG MVGFR+A MYPELVEA+VVTAAP VL E ISRE+LEKIGFKSWSEYLIP++
Sbjct: 121 VLVGFSYGAMVGFRMAEMYPELVEAMVVTAAPTVLMESISRESLEKIGFKSWSEYLIPES 180

Query: 199 AKGVKTLLEIASFKFPRVPRWIFKHYLEAMVIHKTERKELLQALVAADD--------RLH 258
           A+GVK+LLEIASFKFPRVPRWIFKHYLEAMV+HK ER ELL+ LVAADD        +LH
Sbjct: 181 AEGVKSLLEIASFKFPRVPRWIFKHYLEAMVMHKKERAELLRELVAADDVIISQYPQKLH 240

Query: 259 IIWGRNDNLFDVQIAYKMKE 271
           +IWGRNDNLFDVQIAY M E
Sbjct: 241 VIWGRNDNLFDVQIAYNMIE 260

BLAST of Lsi10G003690 vs. NCBI nr
Match: XP_004148865.1 (epoxide hydrolase 3 [Cucumis sativus] >KGN61797.1 hypothetical protein Csa_006154 [Cucumis sativus])

HSP 1 Score: 423.7 bits (1088), Expect = 1.2e-114
Identity = 200/260 (76.92%), Postives = 228/260 (87.69%), Query Frame = 0

Query: 19  MVNIFHVYDRVLRWVMKAAGVRPVQVQIEPGTTMNFWVPAGNPDKNHTNHPPLLLLHGFA 78
           MVNIFH+YD+VLRWVMKAAG++PVQ+QI+PGT MNFWVPAG P+KNH NHPPLL LHGFA
Sbjct: 1   MVNIFHIYDQVLRWVMKAAGIKPVQLQIKPGTAMNFWVPAGKPNKNHQNHPPLLFLHGFA 60

Query: 79  ANAIMTWQFQVLNLAKNYAVYVPDFIFFGESVTDRPDRSPEFQAKCVAEGLRKLGVDRRC 138
            NAIMTWQFQVL  AKN+AVYVPDF+FFG+SVTDRPDR+ EFQA+CV EGLRKLGVDRR 
Sbjct: 61  TNAIMTWQFQVLKFAKNHAVYVPDFMFFGDSVTDRPDRTTEFQAECVVEGLRKLGVDRRF 120

Query: 139 VLVGFSYGVMVGFRLAGMYPELVEAVVVTAAPPVLTELISREALEKIGFKSWSEYLIPKT 198
           VLVGFSYG MVGFRLA MYPE+VEA+VVTAAP VLTE I+ EA+EKIG+KSWSEYLIP+T
Sbjct: 121 VLVGFSYGAMVGFRLAEMYPEMVEAMVVTAAPTVLTERITGEAMEKIGYKSWSEYLIPET 180

Query: 199 AKGVKTLLEIASFKFPRVPRWIFKHYLEAMVIHKTERKELLQALVAADD--------RLH 258
            KG  ++L+IASF+FPR PRWIFK YLEAMV+H+ ER ELL+ALVA +D        +LH
Sbjct: 181 VKGAISMLQIASFEFPRFPRWIFKQYLEAMVVHRKERAELLEALVAPNDVTISQYPQKLH 240

Query: 259 IIWGRNDNLFDVQIAYKMKE 271
           IIWGRNDNLFD+QIAY MKE
Sbjct: 241 IIWGRNDNLFDIQIAYNMKE 260

BLAST of Lsi10G003690 vs. NCBI nr
Match: XP_016902619.1 (PREDICTED: uncharacterized protein LOC103499739 [Cucumis melo])

HSP 1 Score: 423.7 bits (1088), Expect = 1.2e-114
Identity = 202/260 (77.69%), Postives = 226/260 (86.92%), Query Frame = 0

Query: 19  MVNIFHVYDRVLRWVMKAAGVRPVQVQIEPGTTMNFWVPAGNPDKNHTNHPPLLLLHGFA 78
           ++NIFH+YD VLRWVMKAAG+ PVQ+QIEPGT+MNFWVPA  P+KNH NHPPLL LHGFA
Sbjct: 2   VINIFHIYDLVLRWVMKAAGIIPVQLQIEPGTSMNFWVPARKPNKNHQNHPPLLFLHGFA 61

Query: 79  ANAIMTWQFQVLNLAKNYAVYVPDFIFFGESVTDRPDRSPEFQAKCVAEGLRKLGVDRRC 138
           ANAIMTWQFQVL  AK +AVYVPDF+FFG+SVTDRPDRSPEFQA+CVAEGLRKLGV+RR 
Sbjct: 62  ANAIMTWQFQVLKFAKTHAVYVPDFMFFGDSVTDRPDRSPEFQAECVAEGLRKLGVERRF 121

Query: 139 VLVGFSYGVMVGFRLAGMYPELVEAVVVTAAPPVLTELISREALEKIGFKSWSEYLIPKT 198
           VLVGFSYG MVGFRLA  YPELVEA+VVTAAP VLTE I+  ALEKIG+KSWSEYLIP+T
Sbjct: 122 VLVGFSYGAMVGFRLAEKYPELVEAMVVTAAPTVLTERITEAALEKIGYKSWSEYLIPET 181

Query: 199 AKGVKTLLEIASFKFPRVPRWIFKHYLEAMVIHKTERKELLQALVAADD--------RLH 258
            KGVK++LEIASF+FPR PRWIFKHYLE M+IH  ER ELL+ALV  +D        +LH
Sbjct: 182 VKGVKSMLEIASFEFPRFPRWIFKHYLEGMIIHNKERAELLEALVTPNDVTISRYPQKLH 241

Query: 259 IIWGRNDNLFDVQIAYKMKE 271
           IIWGRNDNLFD+QIAY MKE
Sbjct: 242 IIWGRNDNLFDIQIAYNMKE 261

BLAST of Lsi10G003690 vs. NCBI nr
Match: KAA0045542.1 (epoxide hydrolase 4-like [Cucumis melo var. makuwa] >TYK28850.1 epoxide hydrolase 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 384.8 bits (987), Expect = 6.1e-103
Identity = 182/229 (79.48%), Postives = 204/229 (89.08%), Query Frame = 0

Query: 19  MVNIFHVYDRVLRWVMKAAGVRPVQVQIEPGTTMNFWVPAGNPDKNHTNHPPLLLLHGFA 78
           ++NIFH+YD VLRWVMKAAG+ PVQ+QIEPGT+MNFWVPA  P+KNH NHPPLL LHGFA
Sbjct: 2   VINIFHIYDLVLRWVMKAAGIIPVQLQIEPGTSMNFWVPARKPNKNHQNHPPLLFLHGFA 61

Query: 79  ANAIMTWQFQVLNLAKNYAVYVPDFIFFGESVTDRPDRSPEFQAKCVAEGLRKLGVDRRC 138
           ANAIMTWQFQVL  AK +AVYVPDF+FFG+SVTDRPDRSPEFQA+CVAEGLRKLGV+RR 
Sbjct: 62  ANAIMTWQFQVLKFAKTHAVYVPDFMFFGDSVTDRPDRSPEFQAECVAEGLRKLGVERRF 121

Query: 139 VLVGFSYGVMVGFRLAGMYPELVEAVVVTAAPPVLTELISREALEKIGFKSWSEYLIPKT 198
           VLVGFSYG MVGFRLA  YPELVEA+VVTAAP VLTE I+  ALEKIG+KSWSEYLIP+T
Sbjct: 122 VLVGFSYGAMVGFRLAEKYPELVEAMVVTAAPTVLTERITEAALEKIGYKSWSEYLIPET 181

Query: 199 AKGVKTLLEIASFKFPRVPRWIFKHYLEAMVIHKTERKELLQALVAADD 248
            KGVK++LEIASF+FPR PRWIFKHYLE M+IH  ER ELL+ALV  +D
Sbjct: 182 VKGVKSMLEIASFEFPRFPRWIFKHYLEGMIIHNKERAELLEALVTPND 230

BLAST of Lsi10G003690 vs. NCBI nr
Match: XP_022947943.1 (epoxide hydrolase 4-like [Cucurbita moschata])

HSP 1 Score: 375.6 bits (963), Expect = 3.7e-100
Identity = 183/260 (70.38%), Postives = 211/260 (81.15%), Query Frame = 0

Query: 19  MVNIFHVYDRVLRWVMKAAGVRPVQVQIEPGTTMNFWVPAGNPDKNHTNHPPLLLLHGFA 78
           M NIFHVY RVLRW+MK AGVR   VQIEPGTTMNFWVP  NP KN   +PP++LLHGFA
Sbjct: 1   MANIFHVYHRVLRWLMKVAGVRQELVQIEPGTTMNFWVPTRNPKKN--RNPPVVLLHGFA 60

Query: 79  ANAIMTWQFQVLNLAKNYAVYVPDFIFFGESVTDRPDRSPEFQAKCVAEGLRKLGVDRRC 138
            N IMTWQFQV+NLA+NYAVYVPDF+FFGESVTDR +RSPEFQA+ V +GLRKLGV+R C
Sbjct: 61  GNGIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAEGVVKGLRKLGVERPC 120

Query: 139 VLVGFSYGVMVGFRLAGMYPELVEAVVVTAAPPVLTELISREALEKIGFKSWSEYLIPKT 198
           VLVGFSYG MVGFRLA MYP+LV A+VVT+AP  LTE +SR AL +IG+KSWS+YLIP T
Sbjct: 121 VLVGFSYGAMVGFRLAEMYPDLVAAMVVTSAPTALTESLSRVALLEIGYKSWSDYLIPNT 180

Query: 199 AKGVKTLLEIASFKFPRVPRWIFKHYLEAMVIHKTERKELLQALVAADD--------RLH 258
             GVK+L EI SF FP +P WI KHYLEAMV ++ E+ ELL+ALV  DD        ++H
Sbjct: 181 VNGVKSLFEIGSFDFPALPNWIMKHYLEAMVKYRKEQAELLRALVVPDDFTISQFPQKMH 240

Query: 259 IIWGRNDNLFDVQIAYKMKE 271
           IIWGRNDNLF++QIAY MKE
Sbjct: 241 IIWGRNDNLFNMQIAYNMKE 258

BLAST of Lsi10G003690 vs. TAIR 10
Match: AT4G36610.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 232.3 bits (591), Expect = 4.7e-61
Identity = 121/271 (44.65%), Postives = 172/271 (63.47%), Query Frame = 0

Query: 19  MVNIFHVYDRVLRWVMKAAGVRPVQVQIEPGTTMNFWVP---------AGNPDK-NHTNH 78
           MVN   V   +L  +MK AGV P  ++IEPGT +NFWVP          G P K +    
Sbjct: 1   MVNFVEVQKPLLYGLMKMAGVVPYTLEIEPGTKINFWVPKETLKKNSGTGKPTKPDKPKK 60

Query: 79  PPLLLLHGFAANAIMTWQFQVLNLAKNYAVYVPDFIFFGESVTDRPDRSPEFQAKCVAEG 138
           P +LL+HGFA   I+TWQFQV  L+K Y+VY+PD +FFG S TD  DRSP FQA C+ +G
Sbjct: 61  PVVLLIHGFAGEGIVTWQFQVGALSKKYSVYIPDLLFFGGSYTDNSDRSPAFQADCLVKG 120

Query: 139 LRKLGVDRRCVLVGFSYGVMVGFRLAGMYPELVEAVVVTAAPPVLTELISREALEKIGFK 198
           LR LGVD + V VGFSYG MV F++A  YP++V A+VV+ + P +T+ I+  +L ++GF 
Sbjct: 121 LRILGVD-KFVPVGFSYGGMVAFKIAEAYPDMVRAIVVSGSIPTMTDTINEASLNRLGFS 180

Query: 199 SWSEYLIPKTAKGVKTLLEIASFKFPRVPRWIFKHYLEAMVIHKTERKELLQALVAADD- 258
           S ++ L+P +  G+K L  IA  K    P+ +FK Y+E M  ++ ER ELL+A+V ++  
Sbjct: 181 SSTDLLLPTSVTGLKALFTIAVHKPLWFPKRLFKDYIEVMFNNRKERAELLEAVVVSNKE 240

Query: 259 --------RLHIIWGRNDNLFDVQIAYKMKE 271
                   ++H +WG +D +FD+++A  MKE
Sbjct: 241 AQIPHFPRKIHFLWGESDQIFDLELARDMKE 270

BLAST of Lsi10G003690 vs. TAIR 10
Match: AT2G18360.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 231.9 bits (590), Expect = 6.2e-61
Identity = 120/273 (43.96%), Postives = 174/273 (63.74%), Query Frame = 0

Query: 19  MVNIFHVYDRVLRWVMKAAGVRPVQVQIEPGTTMNFWVP------AGNPDKNHT------ 78
           MVN   +   +L  +MK AGV P  V++EPGT MNFW+P          DKN        
Sbjct: 1   MVNFVDLQKPLLYRLMKLAGVIPYTVELEPGTKMNFWIPKETLKKTKKSDKNFAVEPQKP 60

Query: 79  NHPPLLLLHGFAANAIMTWQFQVLNLAKNYAVYVPDFIFFGESVTDRPDRSPEFQAKCVA 138
             P LL +HGFAA  I+TWQFQV +LAK Y+VY+PD +FFG S +D  DRSP FQA C+ 
Sbjct: 61  TKPVLLFIHGFAAEGIVTWQFQVGSLAKKYSVYIPDLLFFGGSYSDNADRSPAFQAHCLV 120

Query: 139 EGLRKLGVDRRCVLVGFSYGVMVGFRLAGMYPELVEAVVVTAAPPVLTELISREALEKIG 198
           + LR LG++ +  LVGFSYG MV F++A  YPE+V+A+VV+ +   +T+ IS   L ++G
Sbjct: 121 KSLRILGIE-KFTLVGFSYGGMVAFKIAEEYPEMVQAMVVSGSILAMTDTISESNLNQLG 180

Query: 199 FKSWSEYLIPKTAKGVKTLLEIASFKFPRVPRWIFKHYLEAMVIHKTERKELLQALVAAD 258
           FKS ++ L+P + KG+KTL  +A  K    P+ +FK ++E M+ ++ ER ELL+ALV ++
Sbjct: 181 FKSSADLLLPTSVKGLKTLFTLAVHKPMWFPKRLFKDFIEVMITNRKERAELLEALVISN 240

Query: 259 ---------DRLHIIWGRNDNLFDVQIAYKMKE 271
                     ++H++WG +D +F+++ A  MKE
Sbjct: 241 KDVTIPRFQQKIHLLWGESDQIFNLEFAKSMKE 272

BLAST of Lsi10G003690 vs. TAIR 10
Match: AT5G21950.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 137.1 bits (344), Expect = 2.1e-32
Identity = 84/265 (31.70%), Postives = 138/265 (52.08%), Query Frame = 0

Query: 18  SMVNIFHVYDRVLRWVMKAAGVRPVQVQIEPGTTMNFWVPAGNPDKNHTNHPPLLLLHGF 77
           S +++    + +LR    +AG+    + I+  TT+ FW P  +    +T  P LLLLHGF
Sbjct: 4   SFLSVARFVEALLRRRFSSAGLSLQTLSIDSETTIQFWGPPPSSSSENTQKPSLLLLHGF 63

Query: 78  AANAIMTWQFQVLNLAKNYAVYVPDFIFFGESVTDRPDRSPEFQAKCVAEGLRKLGVDRR 137
             +A+  W  QV  L+  + +YVPD +FFG S +   +RS  FQA C+ + + KL V+R 
Sbjct: 64  GPSAVWQWSHQVKPLSHFFRLYVPDLVFFGGSSSSGENRSEMFQALCMGKLMEKLEVERF 123

Query: 138 CVLVGFSYGVMVGFRLAGMYPELVEAVVVTAAPPVLTELISREALEKIGFKSWSEYLIPK 197
            V VG SYG  V + +A M+PE VE VV+ ++   L    +   + +       E ++P 
Sbjct: 124 SV-VGTSYGGFVAYNMAKMFPEKVEKVVLASSGVNLRRSDNEAFIARAKCHRIKEVMLPA 183

Query: 198 TAKGVKTLLEIASFK-FPRVPRWIFKHYLEAMVIHKTERK-ELLQAL-VAADDRLHI--- 257
           +A  ++    + S K    VP ++   + + M   K E K ELL+ L +  DD+ ++   
Sbjct: 184 SATDLRRFSGMVSSKRLDYVPDFVLNDFCQKMYSEKREEKAELLEGLSIGKDDKTNVSPI 243

Query: 258 ------IWGRNDNLFDVQIAYKMKE 271
                 IWG  D +F +++A+ +KE
Sbjct: 244 QQDVMLIWGEQDQVFPLKMAHDLKE 267

BLAST of Lsi10G003690 vs. TAIR 10
Match: AT1G78210.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 132.5 bits (332), Expect = 5.1e-31
Identity = 82/262 (31.30%), Postives = 137/262 (52.29%), Query Frame = 0

Query: 27  DRVLRWVMKAAGVRPVQVQIEPGTTMNFWVPAGNPDKNHTNHPPLLLLHGFAANAIMTWQ 86
           +R  +   K +G+RPV + ++ GT +NFWV    P+      P LLL+HG  A AI  W 
Sbjct: 12  ERTYKSGFKRSGLRPVTIDLKDGTVVNFWVSKTKPESK--PKPNLLLIHGLGATAIWQWY 71

Query: 87  FQVLNLAKNYAVYVPDFIFFGESVTDRPDRSPEFQAKCVAEGLRKLGVDRRCVLVGFSYG 146
                L++ + +Y+PD +FFG S T RP+RS  FQA+ +   L    V ++  LVG SYG
Sbjct: 72  DVARRLSRYFNLYIPDLVFFGGSSTTRPERSDIFQAQTLMRALEAQSV-KKFSLVGLSYG 131

Query: 147 VMVGFRLAGMYPELVEAVVVTAAPPVLTELISREALEKIG-FKSWSEYLIPKTAKGVKTL 206
             VG+R+A MY + VE VV+  A   + E   +  + K+      S+ L+P++ K ++ L
Sbjct: 132 GFVGYRMASMYADAVEKVVICCAAVCVEEKDMKAGVFKVSDLDEASKILVPESVKKLREL 191

Query: 207 LEIASFKFPRVPRWIFKHYLEAMVIH------KTERKELLQALVAADDRL---------- 266
           +    +K P + R +    L   + H        E++EL++A+    DR+          
Sbjct: 192 MGYIFYK-PALARLVPTCLLHDFIEHALTRDNMEEKRELIKAI--PKDRIISEIPKLKQP 251

Query: 267 -HIIWGRNDNLFDVQIAYKMKE 271
             IIWG +D +F +++  ++++
Sbjct: 252 TLIIWGEHDQVFPLEMGKRLEK 267

BLAST of Lsi10G003690 vs. TAIR 10
Match: AT4G39955.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 129.8 bits (325), Expect = 3.3e-30
Identity = 87/257 (33.85%), Postives = 131/257 (50.97%), Query Frame = 0

Query: 27  DRVLRWVMKAAGVRPVQVQIEPGTTMNFWVPAGNPDKNHTNHPPLLLLHGFAANAIMTWQ 86
           ++  ++    AG+R     +  GT  + W+P  +    HT  P LLLLHG  ANA+  W 
Sbjct: 12  NKCYQYSFSRAGLRSSTSDLGDGTVFHCWIPLTH---IHTK-PTLLLLHGIGANAMWQWD 71

Query: 87  FQVLNLAKNYAVYVPDFIFFGESVTDRPDRSPEFQAKCVAEGLRKLGVDRRCVLVGFSYG 146
             +      + VYVPD IFFG+S T RPDRS  FQA CV + +   GV R   + G SYG
Sbjct: 72  RFIDRFIPRFNVYVPDLIFFGDSYTTRPDRSESFQATCVMKAMDAYGV-RTMTVAGLSYG 131

Query: 147 VMVGFRLAGMYPELVEAVVVTAAPPVLTELISREALEKIGFKSWSE---YLIPKTAKGVK 206
             V + LA  + E V+ VV+  A   L E  S + + K+  KS  E    L P++   ++
Sbjct: 132 GFVAYSLAAQFKERVDRVVLICAGVALEEKDSEDGMFKV--KSPEEAAAVLFPQSPSMLR 191

Query: 207 TLLEIASFKFP-RVPRWIFKHYLEAMV-IHKTERKELLQALVAADDRLHI---------I 266
            LL+++ +K P  +P      Y+  M   +  ERKEL++AL       ++         I
Sbjct: 192 RLLQLSFYKPPIWIPSCFAMDYIHVMCKDYLQERKELVEALHKGRRFANLPKITQPTLMI 251

Query: 267 WGRNDNLFDVQIAYKMK 270
           WG  D +F V++A+++K
Sbjct: 252 WGEEDQVFPVELAHRLK 261