Lsi10G002530 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi10G002530
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionTMV resistance protein N-like
Locationchr10: 4122177 .. 4124436 (-)
RNA-Seq ExpressionLsi10G002530
SyntenyLsi10G002530
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AGGTGAGGACACTCGCAATTCATTTACGGGTCATCTATATGAAGCTTTGATGCAGGTTGGAATCAATACCTTTAGAGATACAGAGGAGCTGAACAGAGGAATGCAAATTTCGCAAGAACTTTGTGAAGCAATCAACGGGTCGAGGTTTTTTATTGTAGTTTTTTCCGAAAACTATGCATCTTCTAGTTGGTGTTTAGATGAGATTGTTCAAATTATGAGTTGTATGGAGTGTGGTAAAGGGCAGATGGTTTGGCCAGTGTTTTATCATGTGGATCCTTCTGAAGTTAGAAAACAAAATGGCATATTTGGAGAAGCATTCATTGTACATCAACAAAGATTCAAGGAAGACTTGAAAAGGGTTCAAAGTTGGAGAGAAGCTCTAACTAAAGCTTCCAACTTATCCGGATGGCATTTAGATAATATGGGATTTTATCTCAAAACTAATCGGTAATGAGAGGAGTGGTCCATCTATCTTATAAAGATTTTGAAGTTCCTTGATTTTTTCAATAACATTGTAATTTTCTTTCTTTTATTACCTTTTATATAATCTCACTATCTATTGTGATTTTGATGTAGGTCGTGTGACGAGCCTACTCTTGTGAAAAAAATTGTTCGAGAGATATGGACTAAACTTAACAAAACACTCTTAACAGTTGCCAAGAATCCAGTTGGGATCGATTCTCATATTGAAAAACTAAAGTCCATATTGGATTTAGAGTGATGTTCGGATGATTGGAATTTATGGACTTGGTGGAATAGGCAAGACAACTATTGCAAAAGCTATTTATAATTGTATTGCAAATCTATTTGAAGCTTGCAGTTTTCTTCCTACTATAAAAGAGAAATGCGAATTCTCGAGGGATAATGAGCTAACTAAATTGCAAGAATCACTTCTTCAAGACCTCTTGTTGATTAAAATGCGTAGGTCGATAAATTTTTCGGATGAAGGAAGTAATATCTTGAGGGATAGACTAAGAAAGAAAAAGGTGCTTGTTGTTCTTGACGATGTGGATCATTCAAACCAGCTAGAAAAACTAGTAGGACATTTGAGTTGGTTTGGTTTGGGAAGTAGGATTATCATAACAACTAGAAATTTGCACCTTCTAAATAAACACAACATTAAGTGGACATACAAGGTTGATAAATTAAATCATAGAGAAGCACTTGAGTTATTTTGTTGGAATGCATTTAACAATTCTGTTCCTGATGTGAGTTTGGAAGAGCTTTCAAATGTTGCAGTTGAACATGCAGATGGGCTTCCCCTTGCTCTTGCAGTGGTGGGAGCTTCTCTATGCGATTGTATTAAAATTGAGTGGGAATGTCAATTGAAGAAGTTGAAAAAGATTCCAAATCAGGATGTTCACAATAAACTCAAACTAAGTTTTGATGGACTTGGGGATCTTGAGAAGGCAATTTTTCTAGATATTGCATGCTTTTTTAGAGGGTATGAAAAAGAAGATGTGTACTCTTTTTTTGATGCTTGTGATTTCTACCCTCATTCTGGAATTGAATTGCTTATTAAGAAATCTCTTATATATATCGATTACAATAAATTACAAATGCATGACTTGATTCAACTAATGGGTAGAGAGATCGTTCGTCAAGAATCTCCATTAGATCTTGGAAGACGTAGTAGATTGTGGTATCACCAAGATGTTCTTCAAGTGCTACAAGAAAATGAGGTATGATCTTATTCCTAGCCTCTTACTGAGATTTAGAGAAAAATTGGGACATGACATTACAAAACACTGATAAGTTTAAATTAAAAACAAATATATATCTTCTCATCTCTTTGTGGGCGTTGGGGGGTTGAGTTGAGTTCTAAAGTTTGAAGTTAATATGTTGAAGTTAGGAAGTATGTGTTTGAGGTGTAAGAATGTGAAGATGAAGTTCTTCGATAAATGTGAAAAAGAGAAAAAAATGAGAAATATAGTTTGTCAATAAATGTACAAACTTCAATAGTGAATACTATCGAAGTTGGTGGAGTTGAGTTATTTAATACTAACTTATGAAATTGGTAGGCCAAACACTCCCGGCATGTTTTAAAAAGACTATGCTTTCATTTTGATGTATAACATTATCAACTACTTCAAGTGTGAGTGCCTTCATGTGTTTTCAGGGAACAAAAGAAGTTGAAGGGATAATTTTAGAAACGCCCAAAGTAGAAGAAGTAGTGTGGTTGCATGCTAAAGCCTTTTCAAGAATGACAAAATTGAGGTTGCTTATAATACGTAATAATGTTAATGTTATTGGGACTCCT

mRNA sequence

AGGTGAGGACACTCGCAATTCATTTACGGGTCATCTATATGAAGCTTTGATGCAGGTTGGAATCAATACCTTTAGAGATACAGAGGAGCTGAACAGAGGAATGCAAATTTCGCAAGAACTTTGTGAAGCAATCAACGGGTCGAGGTTTTTTATTGTAGTTTTTTCCGAAAACTATGCATCTTCTAGTTGGTGTTTAGATGAGATTGTTCAAATTATGAGTTGTATGGAGTGTGGTAAAGGGCAGATGGTTTGGCCAGTGTTTTATCATGTGGATCCTTCTGAAGTTAGAAAACAAAATGGCATATTTGGAGAAGCATTCATTGTACATCAACAAAGATTCAAGGAAGACTTGAAAAGGAGTGATGTTCGGATGATTGGAATTTATGGACTTGGTGGAATAGGCAAGACAACTATTGCAAAAGCTATTTATAATTGTATTGCAAATCTATTTGAAGCTTGCAGTTTTCTTCCTACTATAAAAGAGAAATGCGAATTCTCGAGGGATAATGAGCTAACTAAATTGCAAGAATCACTTCTTCAAGACCTCTTGTTGATTAAAATGCGTAGGTCGATAAATTTTTCGGATGAAGGAAGTAATATCTTGAGGGATAGACTAAGAAAGAAAAAGGTGCTTGTTGTTCTTGACGATGTGGATCATTCAAACCAGCTAGAAAAACTAGTAGGACATTTGAGTTGGTTTGGTTTGGGAAGTAGGATTATCATAACAACTAGAAATTTGCACCTTCTAAATAAACACAACATTAAGTGGACATACAAGGTTGATAAATTAAATCATAGAGAAGCACTTGAGTTATTTTGTTGGAATGCATTTAACAATTCTGTTCCTGATGTGAGTTTGGAAGAGCTTTCAAATGTTGCAGTTGAACATGCAGATGGGCTTCCCCTTGCTCTTGCAGTGGTGGGAGCTTCTCTATGCGATTGTATTAAAATTGAGTGGGAATGTCAATTGAAGAAGTTGAAAAAGATTCCAAATCAGGATGTTCACAATAAACTCAAACTAAGTTTTGATGGACTTGGGGATCTTGAGAAGGCAATTTTTCTAGATATTGCATGCTTTTTTAGAGGGTATGAAAAAGAAGATGTGTACTCTTTTTTTGATGCTTGTGATTTCTACCCTCATTCTGGAATTGAATTGCTTATTAAGAAATCTCTTATATATATCGATTACAATAAATTACAAATGCATGACTTGATTCAACTAATGGGTAGAGAGATCGTTCGTCAAGAATCTCCATTAGATCTTGGAAGACGTAGTAGATTGTGGTATCACCAAGATGTTCTTCAAGTGCTACAAGAAAATGAGGGAACAAAAGAAGTTGAAGGGATAATTTTAGAAACGCCCAAAGTAGAAGAAGTAGTGTGGTTGCATGCTAAAGCCTTTTCAAGAATGACAAAATTGAGGTTGCTTATAATACGTAATAATGTTAATGTTATTGGGACTCCT

Coding sequence (CDS)

ATGCAGGTTGGAATCAATACCTTTAGAGATACAGAGGAGCTGAACAGAGGAATGCAAATTTCGCAAGAACTTTGTGAAGCAATCAACGGGTCGAGGTTTTTTATTGTAGTTTTTTCCGAAAACTATGCATCTTCTAGTTGGTGTTTAGATGAGATTGTTCAAATTATGAGTTGTATGGAGTGTGGTAAAGGGCAGATGGTTTGGCCAGTGTTTTATCATGTGGATCCTTCTGAAGTTAGAAAACAAAATGGCATATTTGGAGAAGCATTCATTGTACATCAACAAAGATTCAAGGAAGACTTGAAAAGGAGTGATGTTCGGATGATTGGAATTTATGGACTTGGTGGAATAGGCAAGACAACTATTGCAAAAGCTATTTATAATTGTATTGCAAATCTATTTGAAGCTTGCAGTTTTCTTCCTACTATAAAAGAGAAATGCGAATTCTCGAGGGATAATGAGCTAACTAAATTGCAAGAATCACTTCTTCAAGACCTCTTGTTGATTAAAATGCGTAGGTCGATAAATTTTTCGGATGAAGGAAGTAATATCTTGAGGGATAGACTAAGAAAGAAAAAGGTGCTTGTTGTTCTTGACGATGTGGATCATTCAAACCAGCTAGAAAAACTAGTAGGACATTTGAGTTGGTTTGGTTTGGGAAGTAGGATTATCATAACAACTAGAAATTTGCACCTTCTAAATAAACACAACATTAAGTGGACATACAAGGTTGATAAATTAAATCATAGAGAAGCACTTGAGTTATTTTGTTGGAATGCATTTAACAATTCTGTTCCTGATGTGAGTTTGGAAGAGCTTTCAAATGTTGCAGTTGAACATGCAGATGGGCTTCCCCTTGCTCTTGCAGTGGTGGGAGCTTCTCTATGCGATTGTATTAAAATTGAGTGGGAATGTCAATTGAAGAAGTTGAAAAAGATTCCAAATCAGGATGTTCACAATAAACTCAAACTAAGTTTTGATGGACTTGGGGATCTTGAGAAGGCAATTTTTCTAGATATTGCATGCTTTTTTAGAGGGTATGAAAAAGAAGATGTGTACTCTTTTTTTGATGCTTGTGATTTCTACCCTCATTCTGGAATTGAATTGCTTATTAAGAAATCTCTTATATATATCGATTACAATAAATTACAAATGCATGACTTGATTCAACTAATGGGTAGAGAGATCGTTCGTCAAGAATCTCCATTAGATCTTGGAAGACGTAGTAGATTGTGGTATCACCAAGATGTTCTTCAAGTGCTACAAGAAAATGAGGGAACAAAAGAAGTTGAAGGGATAATTTTAGAAACGCCCAAAGTAGAAGAAGTAGTGTGGTTGCATGCTAAAGCCTTTTCAAGAATGACAAAATTGAGGTTGCTTATAATACGTAATAATGTTAATGTTATTGGGACTCCT

Protein sequence

MQVGINTFRDTEELNRGMQISQELCEAINGSRFFIVVFSENYASSSWCLDEIVQIMSCMECGKGQMVWPVFYHVDPSEVRKQNGIFGEAFIVHQQRFKEDLKRSDVRMIGIYGLGGIGKTTIAKAIYNCIANLFEACSFLPTIKEKCEFSRDNELTKLQESLLQDLLLIKMRRSINFSDEGSNILRDRLRKKKVLVVLDDVDHSNQLEKLVGHLSWFGLGSRIIITTRNLHLLNKHNIKWTYKVDKLNHREALELFCWNAFNNSVPDVSLEELSNVAVEHADGLPLALAVVGASLCDCIKIEWECQLKKLKKIPNQDVHNKLKLSFDGLGDLEKAIFLDIACFFRGYEKEDVYSFFDACDFYPHSGIELLIKKSLIYIDYNKLQMHDLIQLMGREIVRQESPLDLGRRSRLWYHQDVLQVLQENEGTKEVEGIILETPKVEEVVWLHAKAFSRMTKLRLLIIRNNVNVIGTP
Homology
BLAST of Lsi10G002530 vs. ExPASy Swiss-Prot
Match: V9M398 (Disease resistance protein RUN1 OS=Vitis rotundifolia OX=103349 GN=RUN1 PE=1 SV=1)

HSP 1 Score: 371.3 bits (952), Expect = 1.6e-101
Identity = 220/532 (41.35%), Postives = 305/532 (57.33%), Query Frame = 0

Query: 4   GINTFRDTEELNRGMQISQELCEAINGSRFFIVVFSENYASSSWCLDEIVQIMSCMECGK 63
           GI TFRD ++L RG  I+ EL +AI  SR  ++VFSENYA S WCLDE+V+IM C +  K
Sbjct: 54  GIRTFRD-DKLRRGEAIAPELLKAIEESRSSVIVFSENYARSRWCLDELVKIMECHKDKK 113

Query: 64  --GQMVWPVFYHVDPSEVRKQNGIFGEAFIVHQQRFKEDLKR------------------ 123
             G  V+P+FYHVDPS VRKQ G FGEAF  + +  K+ + R                  
Sbjct: 114 DPGHAVFPIFYHVDPSHVRKQEGSFGEAFAGYGENLKDKIPRWRTALTEAANLSGWPLQD 173

Query: 124 -----------------------------------------------SDVRMIGIYGLGG 183
                                                          SDVRM+G+YG+GG
Sbjct: 174 GYESNQIKEITDSIFRRLKCKRLDAGANLVGIDSHVKEMIWRLHMESSDVRMVGMYGVGG 233

Query: 184 IGKTTIAKAIYNCIANLFEACSFLPTIKEKCEFSRDNELTKLQESLLQDLLLIKMRRSIN 243
           IGKTTIAK IYN ++  FE  SFL  I+EK        ++ LQ  LL D+L  +  ++IN
Sbjct: 234 IGKTTIAKVIYNELSREFEYMSFLENIREK---FNTQGVSPLQNQLLDDILKGEGSQNIN 293

Query: 244 FSDEGSNILRDRLRKKKVLVVLDDVDHSNQLEKLVGHLSWFGLGSRIIITTRNLHLLNKH 303
               G+++++D L  K V +VLDDVD  +QLE L+ H  W G GSR+IITTRN H+L+  
Sbjct: 294 SVAHGASMIKDILSSKIVFIVLDDVDDQSQLEYLLRHREWLGEGSRVIITTRNKHVLDVQ 353

Query: 304 NIKWTYKVDKLNHREALELFCWNAFNNSVPDVSLEELSNVAVEHADGLPLALAVVGASLC 363
            +   Y+V  LN  EA ELF   AF  ++P      LS+  V +  GLPLAL V+G  L 
Sbjct: 354 KVDDLYEVKGLNFEEACELFSLYAFEQNLPKSDYRNLSHRVVGYCQGLPLALKVLGCLLL 413

Query: 364 DCIKIEWECQLKKLKKIPNQDVHNKLKLSFDGLGDLEKAIFLDIACFFRGYEKEDVYSFF 423
                EWE +L+KL + P  ++ + LK S+DGLG  EK+IFLD+ACFF+G +++ V    
Sbjct: 414 KKTIPEWESELRKLDREPEAEILSVLKRSYDGLGRTEKSIFLDVACFFKGEDRDFVSKIL 473

Query: 424 DACDFYPHSGIELLIKKSLIYIDYNKLQMHDLIQLMGREIVRQESPLDLGRRSRLWYHQD 469
           DACDF+   GI+ L  K LI + YN+++MHDLIQ MG EIVR++ P +  + SRLW   D
Sbjct: 474 DACDFHAEIGIKNLNDKCLITLQYNRIRMHDLIQQMGWEIVREKFPDEPNKWSRLWDTCD 533

BLAST of Lsi10G002530 vs. ExPASy Swiss-Prot
Match: V9M2S5 (Disease resistance protein RPV1 OS=Vitis rotundifolia OX=103349 GN=RPV1 PE=1 SV=1)

HSP 1 Score: 360.9 bits (925), Expect = 2.2e-98
Identity = 217/531 (40.87%), Postives = 297/531 (55.93%), Query Frame = 0

Query: 4   GINTFRDTEELNRGMQISQELCEAINGSRFFIVVFSENYASSSWCLDEIVQIMSCMECGK 63
           GI TFRD + L RG  I+ EL +AI  SR  ++VFSENYA S WCLDE+V+IM C +   
Sbjct: 51  GIRTFRD-DRLRRGEAIAPELLKAIEESRSSVIVFSENYAHSRWCLDELVKIMECQK-DL 110

Query: 64  GQMVWPVFYHVDPSEVRKQNGIFGEAFIVHQQRFKEDLKR-------------------- 123
           G  V+P+FYHVDPS VRKQ G FGEAF  +++ +K+ + R                    
Sbjct: 111 GHAVFPIFYHVDPSHVRKQEGSFGEAFAGYEENWKDKIPRWRTALTEAANLSGWHLLDDR 170

Query: 124 ----------------------------------------------SDVRMIGIYGLGGI 183
                                                         SDVRM+GIYG+GGI
Sbjct: 171 YESNQIKEITNSIFRQLKCKRLDVGANLVGIDSHVKEMILRLHLESSDVRMVGIYGVGGI 230

Query: 184 GKTTIAKAIYNCIANLFEACSFLPTIKEKCEFSRDNELTKLQESLLQDLLLIKMRRSINF 243
           GKTTIAK IYN ++  FE  SFL  I+E    S    L  LQ  LL D+L  +  ++I+ 
Sbjct: 231 GKTTIAKVIYNELSCEFEYMSFLENIREG---SNPQVLFHLQNQLLGDILEGEGSQNISS 290

Query: 244 SDEGSNILRDRLRKKKVLVVLDDVDHSNQLEKLVGHLSWFGLGSRIIITTRNLHLLNKHN 303
               +++++D L  ++V +VLDDVD  +QLE L+GH  W G GSR+IITTRN H+L    
Sbjct: 291 VAHRASMIKDILLSRRVFIVLDDVDDLSQLEYLLGHREWLGEGSRVIITTRNKHVLAVQE 350

Query: 304 IKWTYKVDKLNHREALELFCWNAFNNSVPDVSLEELSNVAVEHADGLPLALAVVGASLCD 363
           +   Y+V+ LN  EA ELF   AF  ++P      L+   V +  GLPLAL V+G+ LC 
Sbjct: 351 VDDLYEVEGLNFEEACELFSLYAFKQNLPKSDYRNLTCRVVGYCQGLPLALKVLGSLLCK 410

Query: 364 CIKIEWECQLKKLKKIPNQDVHNKLKLSFDGLGDLEKAIFLDIACFFRGYEKEDVYSFFD 423
               +WE +LKKL   P  D+H  LK S+DGL  ++K IFLD+ACFF+G  ++ V    D
Sbjct: 411 KTIPQWEGELKKLDSEPKADIHKVLKRSYDGLDRIDKNIFLDLACFFKGEGRDFVLRILD 470

Query: 424 ACDFYPHSGIELLIKKSLIYIDYNKLQMHDLIQLMGREIVRQESPLDLGRRSRLWYHQDV 469
            CDF   +GI  L    LI + YN++ MHDLIQ MG EIVR+  P++  + SRLW   D 
Sbjct: 471 GCDFPAETGISNLNDLCLITLPYNQICMHDLIQQMGWEIVRENFPVEPNKWSRLWDPCDF 530

BLAST of Lsi10G002530 vs. ExPASy Swiss-Prot
Match: A0A290U7C4 (Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 2.2e-90
Identity = 209/536 (38.99%), Postives = 301/536 (56.16%), Query Frame = 0

Query: 1   MQVGINTFRDTEELNRGMQISQELCEAINGSRFFIVVFSENYASSSWCLDEIVQIMSCME 60
           ++ GI+TF D +EL RG  IS EL +AI  SRF +VVFS+NYASS+WCL+E+V+I+   E
Sbjct: 36  IEKGIHTFMDDKELKRGKSISSELMKAIGESRFAVVVFSKNYASSTWCLEELVKILEIHE 95

Query: 61  CGKGQMVWPVFYHVDPSEVRKQNGIFGEAFIVHQQRFKED-------------------- 120
             +  +V PVFY VDPS VRKQNG +   F   +    +D                    
Sbjct: 96  KFE-LIVVPVFYDVDPSTVRKQNGEYAVCFTKFEANLVDDRDKVLRWREALTKVANISGH 155

Query: 121 ------------------------------------------------LKRSD---VRMI 180
                                                           L R D   VR++
Sbjct: 156 DLRNTYNGDESKCIQQILKDIFDKFCFSISITNRDLVGIESQIKKLSSLLRMDLKGVRLV 215

Query: 181 GIYGLGGIGKTTIAKAIYNCIANLFEACSFLPTIKEKCEFSRDNELTKLQESLLQDLLLI 240
           GI+G+GG+GKTT A+A++N     FE+  FL  +K   E+ + + L  LQ++LL  LL +
Sbjct: 216 GIWGMGGVGKTTAARALFNRYYQNFESACFLEDVK---EYLQHHTLLYLQKTLLSKLLKV 275

Query: 241 KMRRSINFSDEGSNILRDRLRKKKVLVVLDDVDHSNQLEKLVGHLSWFGLGSRIIITTRN 300
           +     + ++E   IL+ RL  KKVLVVLDDV+H++QL+KLVG   WFG GSRI+ITTR+
Sbjct: 276 EFVDCTD-TEEMCVILKRRLCSKKVLVVLDDVNHNDQLDKLVGAEDWFGSGSRIVITTRD 335

Query: 301 LHLLNKHNIKWTYKVDKLNHREALELFCWNAFNNSVPDVSLEELSNVAVEHADGLPLALA 360
           + LL  H++  TY++  L   EA+ELF  +AF  S P+   +EL N+ V++  GLPLAL 
Sbjct: 336 MKLLKNHDVHETYEIKVLEKDEAIELFNLHAFKRSSPEKEFKELLNLVVDYTGGLPLALK 395

Query: 361 VVGASLCDCIKIEWECQLKKLKKIPNQDVHNKLKLSFDGLGDLEKAIFLDIACFFRGYEK 420
           V+G+ L       W   + +LK  P  ++   LK+SFDGL D EK+IFLDIACFFRGY +
Sbjct: 396 VLGSLLYKEDLDVWISTIDRLKDNPEGEIMATLKISFDGLRDYEKSIFLDIACFFRGYNQ 455

Query: 421 EDVYSFFDACDFYPHSGIELLIKKSLIYIDYNKLQMHDLIQLMGREIVRQESPLDLGRRS 462
            D+ + F A  F+P  G++ L++KSLI+I  +K+QMHDL+Q MGR+I  QESP+      
Sbjct: 456 RDMTALFHASGFHPVLGVKTLVEKSLIFILEDKIQMHDLMQEMGRQIAVQESPM-----R 515

BLAST of Lsi10G002530 vs. ExPASy Swiss-Prot
Match: Q40392 (TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1)

HSP 1 Score: 313.2 bits (801), Expect = 5.2e-84
Identity = 194/507 (38.26%), Postives = 280/507 (55.23%), Query Frame = 0

Query: 4   GINTFRDTEELNRGMQISQELCEAINGSRFFIVVFSENYASSSWCLDEIVQIMSCMECGK 63
           GI TF+D + L  G  I  ELC+AI  S+F IVVFSENYA+S WCL+E+V+IM C    K
Sbjct: 39  GIKTFQDDKRLEYGATIPGELCKAIEESQFAIVVFSENYATSRWCLNELVKIMECKTRFK 98

Query: 64  GQMVWPVFYHVDPSEVRKQNGIFGEAFIVHQQRFKEDLKR-------------------- 123
            Q V P+FY VDPS VR Q   F +AF  H+ ++K+D++                     
Sbjct: 99  -QTVIPIFYDVDPSHVRNQKESFAKAFEEHETKYKDDVEGIQRWRIALNEAANLKGSCDN 158

Query: 124 -------------------------------------------------SDVRMIGIYGL 183
                                                            + VR++GI+G+
Sbjct: 159 RDKTDADCIRQIVDQISSKLCKISLSYLQNIVGIDTHLEKIESLLEIGINGVRIMGIWGM 218

Query: 184 GGIGKTTIAKAIYNCI------ANLFEACSFLPTIKEKCEFSRDNELTKLQESLLQDLLL 243
           GG+GKTTIA+AI++ +      +  F+   FL  IKE         +  LQ +LL +LL 
Sbjct: 219 GGVGKTTIARAIFDTLLGRMDSSYQFDGACFLKDIKE-----NKRGMHSLQNALLSELL- 278

Query: 244 IKMRRSINFSDEGSNILRDRLRKKKVLVVLDDVDHSNQ-LEKLVGHLSWFGLGSRIIITT 303
            + + + N  ++G + +  RLR KKVL+VLDD+D+ +  LE L G L WFG GSRIIITT
Sbjct: 279 -REKANYNNEEDGKHQMASRLRSKKVLIVLDDIDNKDHYLEYLAGDLDWFGNGSRIIITT 338

Query: 304 RNLHLLNKHNIKWTYKVDKLNHREALELFCWNAFNNSVPDVSLEELSNVAVEHADGLPLA 363
           R+ HL+ K++I   Y+V  L   E+++LF  +AF   VP+ + E+LS   V +A GLPLA
Sbjct: 339 RDKHLIEKNDI--IYEVTALPDHESIQLFKQHAFGKEVPNENFEKLSLEVVNYAKGLPLA 398

Query: 364 LAVVGASLCDCIKIEWECQLKKLKKIPNQDVHNKLKLSFDGLGDLEKAIFLDIACFFRGY 423
           L V G+ L +    EW+  ++ +K      + +KLK+S+DGL   ++ +FLDIACF RG 
Sbjct: 399 LKVWGSLLHNLRLTEWKSAIEHMKNNSYSGIIDKLKISYDGLEPKQQEMFLDIACFLRGE 458

Query: 424 EKEDVYSFFDACDFYPHSGIELLIKKSLIYI-DYNKLQMHDLIQLMGREIVRQESPLDLG 434
           EK+ +    ++C      G+ +LI KSL++I +YN++QMHDLIQ MG+ IV  +   D G
Sbjct: 459 EKDYILQILESCHIGAEYGLRILIDKSLVFISEYNQVQMHDLIQDMGKYIVNFQK--DPG 518

BLAST of Lsi10G002530 vs. ExPASy Swiss-Prot
Match: Q9FI14 (Disease resistance protein TAO1 OS=Arabidopsis thaliana OX=3702 GN=TAO1 PE=3 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 1.1e-73
Identity = 186/526 (35.36%), Postives = 267/526 (50.76%), Query Frame = 0

Query: 4   GINTFRDTEELNRGMQISQELCEAINGSRFFIVVFSENYASSSWCLDEIVQIMSCMECGK 63
           GI  F D  E+ RG  I  EL +AI GS+  I++ S NY SS WCLDE+V+IM C E   
Sbjct: 67  GITPFID-NEMKRGGSIGPELLQAIRGSKIAIILLSRNYGSSKWCLDELVEIMKCRE-EL 126

Query: 64  GQMVWPVFYHVDPSEVRKQNGIFGEAF--------IVHQQRFKE---------------- 123
           GQ V  VFY VDPS+VRKQ G FG+ F            QR+K+                
Sbjct: 127 GQTVMTVFYDVDPSDVRKQKGDFGKVFKKTCVGRPEEMVQRWKQALTSAANILGEDSRNW 186

Query: 124 -------------------------------------------DLKRSDVRMIGIYGLGG 183
                                                       L   +VRMIGI+G  G
Sbjct: 187 ENEADMIIKISKDVSDVLSFTPSKDFDEFVGIEAHTTEITSLLQLDLEEVRMIGIWGPAG 246

Query: 184 IGKTTIAKAIYNCIANLFEACSFLPTIKEKC-EFSRDNELTKLQESLLQDLLLIKMRRSI 243
           IGKTTI++ +YN + + F+  + +  IK +      D    KLQ   LQ  LL +M    
Sbjct: 247 IGKTTISRVLYNKLFHQFQLGAIIDNIKVRYPRPCHDEYSAKLQ---LQKELLSQMINQK 306

Query: 244 NFSDEGSNILRDRLRKKKVLVVLDDVDHSNQLEKLVGHLSWFGLGSRIIITTRNLHLLNK 303
           +       + ++RL+ KKVL+VLDDVD   QL+ +   + WFGLGSRII+ T++L LL  
Sbjct: 307 DMVVPHLGVAQERLKDKKVLLVLDDVDGLVQLDAMAKDVQWFGLGSRIIVVTQDLKLLKA 366

Query: 304 HNIKWTYKVDKLNHREALELFCWNAFNNSVPDVSLEELSNVAVEHADGLPLALAVVGASL 363
           H IK+ YKVD     EALE+FC  AF    P V  E+++      A  LPL L V+G+ L
Sbjct: 367 HGIKYIYKVDFPTSDEALEIFCMYAFGEKSPKVGFEQIARTVTTLAGKLPLGLRVMGSYL 426

Query: 364 CDCIKIEWECQLKKLKKIPNQDVHNKLKLSFDGLGDLEKAIFLDIACFFRGYEKEDVYSF 423
               K EW   + +L+   + D+ + LK S++ L + EK +FL I CFFR    E +  F
Sbjct: 427 RRMSKQEWAKSIPRLRTSLDDDIESVLKFSYNSLAEQEKDLFLHITCFFRRERIETLEVF 486

Query: 424 FDACDFYPHSGIELLIKKSLIYIDYNKLQMHDLIQLMGREIVRQESPLDLGRRSRLWYHQ 461
                     G+++L  KSL+ ++   ++MH+L+  +G +IVR++S    G+R  L   +
Sbjct: 487 LAKKSVDMRQGLQILADKSLLSLNLGNIEMHNLLVQLGLDIVRKQSIHKPGKRQFLVDTE 546

BLAST of Lsi10G002530 vs. ExPASy TrEMBL
Match: A0A5D3C5B5 (TMV resistance protein N-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold70G00650 PE=4 SV=1)

HSP 1 Score: 797.7 bits (2059), Expect = 2.6e-227
Identity = 399/542 (73.62%), Postives = 436/542 (80.44%), Query Frame = 0

Query: 1   MQVGINTFRDTEELNRGMQISQELCEAINGSRFFIVVFSENYASSSWCLDEIVQIMSCME 60
           ++ GIN FRDTEEL RGMQISQELCEAINGSRFF+VVFS+NYASSSWCLDE+VQIMSCME
Sbjct: 35  VRAGINVFRDTEELKRGMQISQELCEAINGSRFFVVVFSKNYASSSWCLDEVVQIMSCME 94

Query: 61  CGKGQMVWPVFYHVDPSEVRKQNGIFGEAFIVHQQRFKEDLKR----------------- 120
            GKGQMVWP+FYH+DPSEVRKQ G+FGEAFIVH++RFKEDLK+                 
Sbjct: 95  GGKGQMVWPLFYHIDPSEVRKQTGLFGEAFIVHKERFKEDLKKVERWREALTKAANLSSW 154

Query: 121 -----------------------------------------------------SDVRMIG 180
                                                                +DVRMIG
Sbjct: 155 HLYDSSCDEATLVKEIVHEISTKLNKTLLTIAKNPVGIGSHIEKLNSALDLESNDVRMIG 214

Query: 181 IYGLGGIGKTTIAKAIYNCIANLFEACSFLPTIKEKCEFSRDNELTKLQESLLQDLLLIK 240
           IYGLGGIGKTT+AKA+YNCIA  FE CSFLP IKEKC FSRD+ELTKLQESLLQD+LLIK
Sbjct: 215 IYGLGGIGKTTVAKAVYNCIAYRFEGCSFLPNIKEKCNFSRDDELTKLQESLLQDILLIK 274

Query: 241 MRRSINFSDEGSNILRDRLRKKKVLVVLDDVDHSNQLEKLVGHLSWFGLGSRIIITTRNL 300
           M RSI+FSDEGSN+LR RLR KKVL+VLDDVDHS+QLEKL GHLSWFG GSRI+ITTR+ 
Sbjct: 275 MHRSISFSDEGSNVLRHRLRNKKVLIVLDDVDHSDQLEKLAGHLSWFGSGSRIMITTRDF 334

Query: 301 HLLNKHNIKWTYKVDKLNHREALELFCWNAFNNSVPDVSLEELSNVAVEHADGLPLALAV 360
           HLLNK NIKWTYKVDKLNHREALELFCWNAFNN VPDVS EELSNVAVEHA+GLPLALAV
Sbjct: 335 HLLNKLNIKWTYKVDKLNHREALELFCWNAFNNPVPDVSFEELSNVAVEHANGLPLALAV 394

Query: 361 VGASLCDCIKIEWECQLKKLKKIPNQDVHNKLKLSFDGLGDLEKAIFLDIACFFRGYEKE 420
           VG SLC+C K+EWE QLKKLKKIPNQDVHNKLKLSFD LGDL+K IFLDIACFFRGY  E
Sbjct: 395 VGGSLCNCSKLEWESQLKKLKKIPNQDVHNKLKLSFDKLGDLQKTIFLDIACFFRGYPTE 454

Query: 421 DVYSFFDACDFYPHSGIELLIKKSLIYIDYNKLQMHDLIQLMGREIVRQESPLDLGRRSR 473
           DVYSF DACDFYP S IE+LIKK LIYI+YN+ QMHDLIQLMG+EIVRQESP D+GRRSR
Sbjct: 455 DVYSFLDACDFYPRSEIEVLIKKCLIYIEYNRFQMHDLIQLMGKEIVRQESPSDVGRRSR 514

BLAST of Lsi10G002530 vs. ExPASy TrEMBL
Match: A0A1S4E327 (TMV resistance protein N-like OS=Cucumis melo OX=3656 GN=LOC103498360 PE=4 SV=1)

HSP 1 Score: 797.7 bits (2059), Expect = 2.6e-227
Identity = 399/542 (73.62%), Postives = 436/542 (80.44%), Query Frame = 0

Query: 1   MQVGINTFRDTEELNRGMQISQELCEAINGSRFFIVVFSENYASSSWCLDEIVQIMSCME 60
           ++ GIN FRDTEEL RGMQISQELCEAINGSRFF+VVFS+NYASSSWCLDE+VQIMSCME
Sbjct: 35  VRAGINVFRDTEELKRGMQISQELCEAINGSRFFVVVFSKNYASSSWCLDEVVQIMSCME 94

Query: 61  CGKGQMVWPVFYHVDPSEVRKQNGIFGEAFIVHQQRFKEDLKR----------------- 120
            GKGQMVWP+FYH+DPSEVRKQ G+FGEAFIVH++RFKEDLK+                 
Sbjct: 95  GGKGQMVWPLFYHIDPSEVRKQTGLFGEAFIVHKERFKEDLKKVERWREALTKAANLSSW 154

Query: 121 -----------------------------------------------------SDVRMIG 180
                                                                +DVRMIG
Sbjct: 155 HLYDSSCDEATLVKEIVHEISTKLNKTLLTIAKNPVGIGSHIEKLNSALDLESNDVRMIG 214

Query: 181 IYGLGGIGKTTIAKAIYNCIANLFEACSFLPTIKEKCEFSRDNELTKLQESLLQDLLLIK 240
           IYGLGGIGKTT+AKA+YNCIA  FE CSFLP IKEKC FSRD+ELTKLQESLLQD+LLIK
Sbjct: 215 IYGLGGIGKTTVAKAVYNCIAYRFEGCSFLPNIKEKCNFSRDDELTKLQESLLQDILLIK 274

Query: 241 MRRSINFSDEGSNILRDRLRKKKVLVVLDDVDHSNQLEKLVGHLSWFGLGSRIIITTRNL 300
           M RSI+FSDEGSN+LR RLR KKVL+VLDDVDHS+QLEKL GHLSWFG GSRI+ITTR+ 
Sbjct: 275 MHRSISFSDEGSNVLRHRLRNKKVLIVLDDVDHSDQLEKLAGHLSWFGSGSRIMITTRDF 334

Query: 301 HLLNKHNIKWTYKVDKLNHREALELFCWNAFNNSVPDVSLEELSNVAVEHADGLPLALAV 360
           HLLNK NIKWTYKVDKLNHREALELFCWNAFNN VPDVS EELSNVAVEHA+GLPLALAV
Sbjct: 335 HLLNKLNIKWTYKVDKLNHREALELFCWNAFNNPVPDVSFEELSNVAVEHANGLPLALAV 394

Query: 361 VGASLCDCIKIEWECQLKKLKKIPNQDVHNKLKLSFDGLGDLEKAIFLDIACFFRGYEKE 420
           VG SLC+C K+EWE QLKKLKKIPNQDVHNKLKLSFD LGDL+K IFLDIACFFRGY  E
Sbjct: 395 VGGSLCNCSKLEWESQLKKLKKIPNQDVHNKLKLSFDKLGDLQKTIFLDIACFFRGYPTE 454

Query: 421 DVYSFFDACDFYPHSGIELLIKKSLIYIDYNKLQMHDLIQLMGREIVRQESPLDLGRRSR 473
           DVYSF DACDFYP S IE+LIKK LIYI+YN+ QMHDLIQLMG+EIVRQESP D+GRRSR
Sbjct: 455 DVYSFLDACDFYPRSEIEVLIKKCLIYIEYNRFQMHDLIQLMGKEIVRQESPSDVGRRSR 514

BLAST of Lsi10G002530 vs. ExPASy TrEMBL
Match: A0A5A7TN33 (TMV resistance protein N-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold75G00650 PE=4 SV=1)

HSP 1 Score: 662.1 bits (1707), Expect = 1.7e-186
Identity = 340/496 (68.55%), Postives = 368/496 (74.19%), Query Frame = 0

Query: 1   MQVGINTFRDTEELNRGMQISQELCEAINGSRFFIVVFSENYASSSWCLDEIVQIMSCME 60
           ++ GIN FRDTEEL RGMQISQELCEAINGSRFF+V FS+NYASSSWCLDE+VQIMSCME
Sbjct: 35  VRAGINVFRDTEELKRGMQISQELCEAINGSRFFVVAFSKNYASSSWCLDEVVQIMSCME 94

Query: 61  CGKGQMVWPVFYHVDPSEVRKQNGIFGEAFIVHQQRFKEDLKR----------------- 120
            GKGQMVWP+FYH+DPSEVRKQ G+FGEA+IVH++RFKEDLK+                 
Sbjct: 95  GGKGQMVWPLFYHIDPSEVRKQTGVFGEAYIVHKERFKEDLKKVERWREALTKAANLSSW 154

Query: 121 -----------------------------------------------------SDVRMIG 180
                                                                +DVRMIG
Sbjct: 155 HLYDSSCDEATLVKEIVHEISTKLNKTLLTIAKNPVGISSHIEKLNSALDLETNDVRMIG 214

Query: 181 IYGLGGIGKTTIAKAIYNCIANLFEACSFLPTIKEKCEFSRDNELTKLQESLLQDLLLIK 240
           IYGLGGIGKTT+AKA+YNCIA  FE CSFLP IKEKC FSRD+ELTKLQESLLQD+LLIK
Sbjct: 215 IYGLGGIGKTTVAKAVYNCIAYRFEGCSFLPNIKEKCNFSRDDELTKLQESLLQDILLIK 274

Query: 241 MRRSINFSDEGSNILRDRLRKKKVLVVLDDVDHSNQLEKLVGHLSWFGLGSRIIITTRNL 300
           M RSI+FSDEG                       NQ EKLVGHLSWFG GSRIIITTR+ 
Sbjct: 275 MHRSISFSDEG-----------------------NQQEKLVGHLSWFGSGSRIIITTRDF 334

Query: 301 HLLNKHNIKWTYKVDKLNHREALELFCWNAFNNSVPDVSLEELSNVAVEHADGLPLALAV 360
           HLLNK NIKWTYKVDKLNHREALELFCWNAFNN VPDVS EELSNVAVE A+GLPLAL V
Sbjct: 335 HLLNKLNIKWTYKVDKLNHREALELFCWNAFNNPVPDVSFEELSNVAVEQANGLPLALVV 394

Query: 361 VGASLCDCIKIEWECQLKKLKKIPNQDVHNKLKLSFDGLGDLEKAIFLDIACFFRGYEKE 420
           VG SLC+C K+EWE QLKKLKKIPNQDVHNKLKLSFD LGDL+K IFLDIACFFRGY  E
Sbjct: 395 VGGSLCNCSKLEWESQLKKLKKIPNQDVHNKLKLSFDRLGDLQKTIFLDIACFFRGYPTE 454

Query: 421 DVYSFFDACDFYPHSGIELLIKKSLIYIDYNKLQMHDLIQLMGREIVRQESPLDLGRRSR 427
           DVYSF DACDFY  S IE+LIKK LIYI+YN+ QMHDLIQLMG+EIVRQESP DLGRRSR
Sbjct: 455 DVYSFIDACDFYTRSEIEVLIKKCLIYIEYNRFQMHDLIQLMGKEIVRQESPSDLGRRSR 506

BLAST of Lsi10G002530 vs. ExPASy TrEMBL
Match: A0A6J1D4F5 (TMV resistance protein N-like OS=Momordica charantia OX=3673 GN=LOC111017198 PE=4 SV=1)

HSP 1 Score: 584.3 bits (1505), Expect = 4.5e-163
Identity = 317/543 (58.38%), Postives = 371/543 (68.32%), Query Frame = 0

Query: 2   QVGINTFRDTEELNRGMQISQELCEAINGSRFFIVVFSENYASSSWCLDEIVQIMSCMEC 61
           Q GIN F+D+EEL RG++ISQE+ EAI+GSR  +VVFS+NYASS+WCL+E+ +IM CME 
Sbjct: 31  QAGINAFKDSEELKRGIEISQEIREAIDGSRILVVVFSKNYASSTWCLEEL-RIMGCMER 90

Query: 62  GKGQMVWPVFYHVDPSEVRKQNGIFGEAFIVHQQRFKEDLKR------------------ 121
             GQMV PVFY VDPS+V+KQ+  FG AF  H++ FKEDL++                  
Sbjct: 91  PNGQMVLPVFYDVDPSQVQKQD--FGGAFTQHEETFKEDLEKVQKWRRALTKAANISGWH 150

Query: 122 --------------------------------------------------SDVRMIGIYG 181
                                                              DVRMIGIYG
Sbjct: 151 LNHRSESTLIKDIVQDILTKINRTLLTIAKNPVGIDFHIQQLKSALGLELDDVRMIGIYG 210

Query: 182 LGGIGKTTIAKAIYNCIANLFEACSFLPTIKEKCEFSRDNELTKLQESLLQDLLLIKMRR 241
           LGGIGKTTIAKAIYN IAN F+ CSFLP+IKEKC  S  + L KLQE LLQD+LL KM  
Sbjct: 211 LGGIGKTTIAKAIYNLIANRFDGCSFLPSIKEKCGSSMHDGLVKLQELLLQDILLDKMHW 270

Query: 242 SINFSDEGSNILRDRLRKKKVLVVLDDVDHSNQLEKLVGHLSWFGLGSRIIITTRNLHLL 301
           SINF D G+NILR+RLRKKKVL+VLDDVD+   LEKL G+  WFG GSR+IITTRN  LL
Sbjct: 271 SINFVDRGTNILRERLRKKKVLIVLDDVDNILHLEKLAGNGDWFGRGSRVIITTRNKQLL 330

Query: 302 NKHNIKWTYKVDKLNHREALELFCWNAFNNSVPDVSLEELSNVAVEHADGLPLALAVVGA 361
           + H I WTYKVD+LNH +ALELFCWNAF    P+V+ EELS+  V +ADG PLAL VVGA
Sbjct: 331 DAHGIYWTYKVDELNHEDALELFCWNAFKKPHPNVNFEELSDAVVRYADGHPLALTVVGA 390

Query: 362 SLCDCIKIEWECQLKKLKKIPNQDVHNKLKLSFDGLGDLEKAIFLDIACFFRGYEKEDVY 421
           SLC C K +WE +L+KL++IP +DVH  LKLSFDGL D+EK IFLDIACFF+G E   V 
Sbjct: 391 SLCGCSKAQWESELQKLRRIPKKDVHEMLKLSFDGLDDVEKTIFLDIACFFKGEEVGRVM 450

Query: 422 SFFDACDFYPHSGIELLIKKSLIYID----YNKLQMHDLIQLMGREIVRQESPLDLGRRS 473
           SF DAC FYP SGIE+L KK LIY +     +  QMHD+IQLMGREIVRQESP DLGRRS
Sbjct: 451 SFLDACGFYPDSGIEVLKKKCLIYSEEMFLTSTFQMHDMIQLMGREIVRQESPFDLGRRS 510

BLAST of Lsi10G002530 vs. ExPASy TrEMBL
Match: A0A6J1D393 (TMV resistance protein N-like OS=Momordica charantia OX=3673 GN=LOC111017199 PE=4 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 1.9e-134
Identity = 262/461 (56.83%), Postives = 305/461 (66.16%), Query Frame = 0

Query: 2   QVGINTFRDTEELNRGMQISQELCEAINGSRFFIVVFSENYASSSWCLDEIVQIMSCMEC 61
           Q GIN FRD+EEL RG++IS+ELCEAI+GSRF +VVFS+NYASS+WCL+E+V+IM CME 
Sbjct: 31  QAGINAFRDSEELQRGVEISEELCEAIDGSRFLVVVFSKNYASSTWCLEELVRIMGCMER 90

Query: 62  GKGQMVWPVFYHVDPSEVRKQNGIFGEAFIVHQQRFKEDLKR------------------ 121
             GQMV PVFY VDPSEVRKQ+G FGEAF  H++RFKEDL++                  
Sbjct: 91  LNGQMVLPVFYDVDPSEVRKQDGGFGEAFTKHEERFKEDLEKVQRWRKALTEAANLSGWH 150

Query: 122 --------------------------------------------------SDVRMIGIYG 181
                                                              DVRMIGIYG
Sbjct: 151 LNQRCESTFIKNVVQEILDKINSTLLTIATNPVGIDSHIQQLESVLGLEFDDVRMIGIYG 210

Query: 182 LGGIGKTTIAKAIYNCIANLFEACSFLPTIKEKCEFSRDNELTKLQESLLQDLLLIKMRR 241
           LGGIGKTTIAKAIYN IAN F+ CSFLP ++EKC FS  + L KLQE LLQD+LL+KM  
Sbjct: 211 LGGIGKTTIAKAIYNSIANRFDGCSFLPNVREKCGFSMHDGLVKLQELLLQDILLVKMHW 270

Query: 242 SINFSDEGSNILRDRLRKKKVLVVLDDVDHSNQLEKLVGHLSWFGLGSRIIITTRNLHLL 301
           SINF D G+NILRDRLR KKVL+VLDDVD+   LEKL G++ WFG GSRIIITTRN  LL
Sbjct: 271 SINFVDRGTNILRDRLRNKKVLIVLDDVDNIFHLEKLAGNVDWFGPGSRIIITTRNKQLL 330

Query: 302 NKHNIKWTYKVDKLNHREALELFCWNAFNNSVPDVSLEELSNVAVEHADGLPLALAVVGA 361
           + H I  TYKVD LNH +AL+LFCWNAF  S PDV+ +ELS+  V +ADG PLAL VVGA
Sbjct: 331 DAHGIYLTYKVDGLNHEDALKLFCWNAFKKSDPDVNFDELSDAVVRYADGHPLALTVVGA 390

Query: 362 SLCDCIKIEWECQLKKLKKIPNQDVHNKLKLSFDGLGDLEKAIFLDIACFFRGYEKEDVY 395
           SLC   K +WE +L+KL++IP QDV N LKLSFDGL DL+K IFLDIACFF+G E   V 
Sbjct: 391 SLCGWNKAQWESELQKLRRIPKQDVQNILKLSFDGLDDLQKEIFLDIACFFKGEEVGCVM 450

BLAST of Lsi10G002530 vs. NCBI nr
Match: XP_038901313.1 (disease resistance protein RUN1-like [Benincasa hispida])

HSP 1 Score: 799.3 bits (2063), Expect = 1.8e-227
Identity = 403/542 (74.35%), Postives = 436/542 (80.44%), Query Frame = 0

Query: 1   MQVGINTFRDTEELNRGMQISQELCEAINGSRFFIVVFSENYASSSWCLDEIVQIMSCME 60
           +QVGINTFRDTEEL RG +ISQELCEAI GSRFF+VVFSENYASSSWCLDE+VQIM+C+E
Sbjct: 34  VQVGINTFRDTEELKRGTEISQELCEAIKGSRFFLVVFSENYASSSWCLDEVVQIMNCVE 93

Query: 61  CGKGQMVWPVFYHVDPSEVRKQNGIFGEAFIVHQQRFKEDLKR----------------- 120
            GKGQMVWPVFYHVDPS+VRKQ G FGE FI+ ++RFKEDLKR                 
Sbjct: 94  SGKGQMVWPVFYHVDPSQVRKQKGTFGEPFILQKERFKEDLKRVERWREALTKVANLSGT 153

Query: 121 -----------------------------------------------------SDVRMIG 180
                                                                +DVRMIG
Sbjct: 154 HLDNRSSDEPTIIKEIVQEIWIKLNKTLLIVAKNPVGIGSHIEKLKSTLDIESNDVRMIG 213

Query: 181 IYGLGGIGKTTIAKAIYNCIANLFEACSFLPTIKEKCEFSRDNELTKLQESLLQDLLLIK 240
           IYGLGGIGKTTIAKAIYNCIA+ FEAC+FLP IKEKC+FSRD+ELT+LQESLL+D+LLIK
Sbjct: 214 IYGLGGIGKTTIAKAIYNCIAHRFEACTFLPNIKEKCKFSRDDELTQLQESLLEDILLIK 273

Query: 241 MRRSINFSDEGSNILRDRLRKKKVLVVLDDVDHSNQLEKLVGHLSWFGLGSRIIITTRNL 300
           + RSI FSD+GSN+LRDRLR KKVLVVLDDVDHSNQLEKL GHLSWFG GSRIIITTR+L
Sbjct: 274 IHRSIKFSDKGSNMLRDRLRNKKVLVVLDDVDHSNQLEKLTGHLSWFGSGSRIIITTRDL 333

Query: 301 HLLNKHNIKWTYKVDKLNHREALELFCWNAFNNSVPDVSLEELSNVAVEHADGLPLALAV 360
           HLLNKHNIKWTYKVD+LNHREALELFCWNAFNN VPDVS EELSNVAV+HADGLPLAL V
Sbjct: 334 HLLNKHNIKWTYKVDELNHREALELFCWNAFNNPVPDVSFEELSNVAVKHADGLPLALEV 393

Query: 361 VGASLCDCIKIEWECQLKKLKKIPNQDVHNKLKLSFDGLGDLEKAIFLDIACFFRGYEKE 420
           VGASLC+C K+EWE QLKKLKKIPNQDVHNKLKLSFDGLG+LEKAIFLDIACFFRGY  E
Sbjct: 394 VGASLCNCSKLEWESQLKKLKKIPNQDVHNKLKLSFDGLGELEKAIFLDIACFFRGYSTE 453

Query: 421 DVYSFFDACDFYPHSGIELLIKKSLIYIDYNKLQMHDLIQLMGREIVRQESPLDLGRRSR 473
           DV SFFD CDFYPH GI +LIKKSLIYID +  +MHDLIQLMGREIVRQESP D GRRSR
Sbjct: 454 DVNSFFDVCDFYPHYGIGVLIKKSLIYIDRDTFEMHDLIQLMGREIVRQESPSDPGRRSR 513

BLAST of Lsi10G002530 vs. NCBI nr
Match: TYK06530.1 (TMV resistance protein N-like [Cucumis melo var. makuwa])

HSP 1 Score: 797.7 bits (2059), Expect = 5.3e-227
Identity = 399/542 (73.62%), Postives = 436/542 (80.44%), Query Frame = 0

Query: 1   MQVGINTFRDTEELNRGMQISQELCEAINGSRFFIVVFSENYASSSWCLDEIVQIMSCME 60
           ++ GIN FRDTEEL RGMQISQELCEAINGSRFF+VVFS+NYASSSWCLDE+VQIMSCME
Sbjct: 35  VRAGINVFRDTEELKRGMQISQELCEAINGSRFFVVVFSKNYASSSWCLDEVVQIMSCME 94

Query: 61  CGKGQMVWPVFYHVDPSEVRKQNGIFGEAFIVHQQRFKEDLKR----------------- 120
            GKGQMVWP+FYH+DPSEVRKQ G+FGEAFIVH++RFKEDLK+                 
Sbjct: 95  GGKGQMVWPLFYHIDPSEVRKQTGLFGEAFIVHKERFKEDLKKVERWREALTKAANLSSW 154

Query: 121 -----------------------------------------------------SDVRMIG 180
                                                                +DVRMIG
Sbjct: 155 HLYDSSCDEATLVKEIVHEISTKLNKTLLTIAKNPVGIGSHIEKLNSALDLESNDVRMIG 214

Query: 181 IYGLGGIGKTTIAKAIYNCIANLFEACSFLPTIKEKCEFSRDNELTKLQESLLQDLLLIK 240
           IYGLGGIGKTT+AKA+YNCIA  FE CSFLP IKEKC FSRD+ELTKLQESLLQD+LLIK
Sbjct: 215 IYGLGGIGKTTVAKAVYNCIAYRFEGCSFLPNIKEKCNFSRDDELTKLQESLLQDILLIK 274

Query: 241 MRRSINFSDEGSNILRDRLRKKKVLVVLDDVDHSNQLEKLVGHLSWFGLGSRIIITTRNL 300
           M RSI+FSDEGSN+LR RLR KKVL+VLDDVDHS+QLEKL GHLSWFG GSRI+ITTR+ 
Sbjct: 275 MHRSISFSDEGSNVLRHRLRNKKVLIVLDDVDHSDQLEKLAGHLSWFGSGSRIMITTRDF 334

Query: 301 HLLNKHNIKWTYKVDKLNHREALELFCWNAFNNSVPDVSLEELSNVAVEHADGLPLALAV 360
           HLLNK NIKWTYKVDKLNHREALELFCWNAFNN VPDVS EELSNVAVEHA+GLPLALAV
Sbjct: 335 HLLNKLNIKWTYKVDKLNHREALELFCWNAFNNPVPDVSFEELSNVAVEHANGLPLALAV 394

Query: 361 VGASLCDCIKIEWECQLKKLKKIPNQDVHNKLKLSFDGLGDLEKAIFLDIACFFRGYEKE 420
           VG SLC+C K+EWE QLKKLKKIPNQDVHNKLKLSFD LGDL+K IFLDIACFFRGY  E
Sbjct: 395 VGGSLCNCSKLEWESQLKKLKKIPNQDVHNKLKLSFDKLGDLQKTIFLDIACFFRGYPTE 454

Query: 421 DVYSFFDACDFYPHSGIELLIKKSLIYIDYNKLQMHDLIQLMGREIVRQESPLDLGRRSR 473
           DVYSF DACDFYP S IE+LIKK LIYI+YN+ QMHDLIQLMG+EIVRQESP D+GRRSR
Sbjct: 455 DVYSFLDACDFYPRSEIEVLIKKCLIYIEYNRFQMHDLIQLMGKEIVRQESPSDVGRRSR 514

BLAST of Lsi10G002530 vs. NCBI nr
Match: XP_016902380.1 (PREDICTED: TMV resistance protein N-like [Cucumis melo])

HSP 1 Score: 797.7 bits (2059), Expect = 5.3e-227
Identity = 399/542 (73.62%), Postives = 436/542 (80.44%), Query Frame = 0

Query: 1   MQVGINTFRDTEELNRGMQISQELCEAINGSRFFIVVFSENYASSSWCLDEIVQIMSCME 60
           ++ GIN FRDTEEL RGMQISQELCEAINGSRFF+VVFS+NYASSSWCLDE+VQIMSCME
Sbjct: 35  VRAGINVFRDTEELKRGMQISQELCEAINGSRFFVVVFSKNYASSSWCLDEVVQIMSCME 94

Query: 61  CGKGQMVWPVFYHVDPSEVRKQNGIFGEAFIVHQQRFKEDLKR----------------- 120
            GKGQMVWP+FYH+DPSEVRKQ G+FGEAFIVH++RFKEDLK+                 
Sbjct: 95  GGKGQMVWPLFYHIDPSEVRKQTGLFGEAFIVHKERFKEDLKKVERWREALTKAANLSSW 154

Query: 121 -----------------------------------------------------SDVRMIG 180
                                                                +DVRMIG
Sbjct: 155 HLYDSSCDEATLVKEIVHEISTKLNKTLLTIAKNPVGIGSHIEKLNSALDLESNDVRMIG 214

Query: 181 IYGLGGIGKTTIAKAIYNCIANLFEACSFLPTIKEKCEFSRDNELTKLQESLLQDLLLIK 240
           IYGLGGIGKTT+AKA+YNCIA  FE CSFLP IKEKC FSRD+ELTKLQESLLQD+LLIK
Sbjct: 215 IYGLGGIGKTTVAKAVYNCIAYRFEGCSFLPNIKEKCNFSRDDELTKLQESLLQDILLIK 274

Query: 241 MRRSINFSDEGSNILRDRLRKKKVLVVLDDVDHSNQLEKLVGHLSWFGLGSRIIITTRNL 300
           M RSI+FSDEGSN+LR RLR KKVL+VLDDVDHS+QLEKL GHLSWFG GSRI+ITTR+ 
Sbjct: 275 MHRSISFSDEGSNVLRHRLRNKKVLIVLDDVDHSDQLEKLAGHLSWFGSGSRIMITTRDF 334

Query: 301 HLLNKHNIKWTYKVDKLNHREALELFCWNAFNNSVPDVSLEELSNVAVEHADGLPLALAV 360
           HLLNK NIKWTYKVDKLNHREALELFCWNAFNN VPDVS EELSNVAVEHA+GLPLALAV
Sbjct: 335 HLLNKLNIKWTYKVDKLNHREALELFCWNAFNNPVPDVSFEELSNVAVEHANGLPLALAV 394

Query: 361 VGASLCDCIKIEWECQLKKLKKIPNQDVHNKLKLSFDGLGDLEKAIFLDIACFFRGYEKE 420
           VG SLC+C K+EWE QLKKLKKIPNQDVHNKLKLSFD LGDL+K IFLDIACFFRGY  E
Sbjct: 395 VGGSLCNCSKLEWESQLKKLKKIPNQDVHNKLKLSFDKLGDLQKTIFLDIACFFRGYPTE 454

Query: 421 DVYSFFDACDFYPHSGIELLIKKSLIYIDYNKLQMHDLIQLMGREIVRQESPLDLGRRSR 473
           DVYSF DACDFYP S IE+LIKK LIYI+YN+ QMHDLIQLMG+EIVRQESP D+GRRSR
Sbjct: 455 DVYSFLDACDFYPRSEIEVLIKKCLIYIEYNRFQMHDLIQLMGKEIVRQESPSDVGRRSR 514

BLAST of Lsi10G002530 vs. NCBI nr
Match: XP_038902439.1 (disease resistance protein RUN1-like [Benincasa hispida])

HSP 1 Score: 793.9 bits (2049), Expect = 7.6e-226
Identity = 404/544 (74.26%), Postives = 441/544 (81.07%), Query Frame = 0

Query: 1   MQVGINTFRDTEELNRGMQISQELCEAINGSRFFIVVFSENYASSSWCLDEIVQIMSCME 60
           +QVGINTFRDTEEL RG +ISQELCEAI  SRFF+V+FSENYASSSWCLDEIV+IM+C+E
Sbjct: 34  VQVGINTFRDTEELKRGTEISQELCEAIKESRFFLVIFSENYASSSWCLDEIVEIMNCVE 93

Query: 61  CGKGQMVWPVFYHVDPSEVRKQNGIFGEAFIVHQQRFKEDLKR----------------- 120
            GKGQMVWPVFYHVDPS+VRKQ G+FGEAFI+H+QRFKEDLK+                 
Sbjct: 94  SGKGQMVWPVFYHVDPSQVRKQKGVFGEAFILHKQRFKEDLKKVERWREALTKAANLSGW 153

Query: 121 -----------------------------------------------------SDVRMIG 180
                                                                +DVRMIG
Sbjct: 154 HLDNRSCDEPTLIKEIVPEIWTKLNKILLTVAKNPVGIDSHIENLKSTLALESNDVRMIG 213

Query: 181 IYGLGGIGKTTIAKAIYNCIANLFEACSFLPTIKEKCEFSRDNELTKLQESLLQDLLLIK 240
           IYGLGGIGK+TIAKAIYNCIA+ FEAC+FLP IKEKC+FSRD+ELT+LQESLL+D+LLIK
Sbjct: 214 IYGLGGIGKSTIAKAIYNCIAHRFEACTFLPNIKEKCKFSRDDELTQLQESLLEDILLIK 273

Query: 241 MRRSINFSDEGSNILRDRLRKKKVLVVLDDVDHSNQLEKLVGHLSWFGLGSRIIITTRNL 300
           + R + FSDEGSN+LRDRLR KKVLVVLDDVDHSNQLEKL GHLSWFG GSRIIITTR+L
Sbjct: 274 IHRLVKFSDEGSNMLRDRLRNKKVLVVLDDVDHSNQLEKLAGHLSWFGSGSRIIITTRDL 333

Query: 301 HLLNKHNIKWTYKVDKLNHREALELFCWNAFNNSVPDVSLEELSNVAVEHADGLPLALAV 360
           HLLNKHNIKWTYKVDKLNHREALELFCWNAFNN VPDVS EELSNVAVE+A+GLPLAL V
Sbjct: 334 HLLNKHNIKWTYKVDKLNHREALELFCWNAFNNPVPDVSFEELSNVAVEYANGLPLALVV 393

Query: 361 VGASLCDCIKIEWECQLKKLKKIPNQDVHNKLKLSFDGLGDLEKAIFLDIACFFRGYEKE 420
           VGASLC+C KIEWE QLKKLKKI NQDVHN+LKLSFDGLG+LEKAIFLDIACFFRGYE E
Sbjct: 394 VGASLCNCSKIEWESQLKKLKKIQNQDVHNRLKLSFDGLGELEKAIFLDIACFFRGYETE 453

Query: 421 DVYSFFDACDFYPHSGIELLIKKSLIYIDYNKLQMHDLIQLMGREIVRQESPLDLGRRSR 473
           DVYSF DACDFYP  GI +LIKK LIYI+YN+ QMHDLIQLMGREIVRQESP ++GRRSR
Sbjct: 454 DVYSFLDACDFYPQYGIGVLIKKCLIYIEYNRFQMHDLIQLMGREIVRQESPSNIGRRSR 513

BLAST of Lsi10G002530 vs. NCBI nr
Match: KAA0042991.1 (TMV resistance protein N-like [Cucumis melo var. makuwa])

HSP 1 Score: 662.1 bits (1707), Expect = 3.5e-186
Identity = 340/496 (68.55%), Postives = 368/496 (74.19%), Query Frame = 0

Query: 1   MQVGINTFRDTEELNRGMQISQELCEAINGSRFFIVVFSENYASSSWCLDEIVQIMSCME 60
           ++ GIN FRDTEEL RGMQISQELCEAINGSRFF+V FS+NYASSSWCLDE+VQIMSCME
Sbjct: 35  VRAGINVFRDTEELKRGMQISQELCEAINGSRFFVVAFSKNYASSSWCLDEVVQIMSCME 94

Query: 61  CGKGQMVWPVFYHVDPSEVRKQNGIFGEAFIVHQQRFKEDLKR----------------- 120
            GKGQMVWP+FYH+DPSEVRKQ G+FGEA+IVH++RFKEDLK+                 
Sbjct: 95  GGKGQMVWPLFYHIDPSEVRKQTGVFGEAYIVHKERFKEDLKKVERWREALTKAANLSSW 154

Query: 121 -----------------------------------------------------SDVRMIG 180
                                                                +DVRMIG
Sbjct: 155 HLYDSSCDEATLVKEIVHEISTKLNKTLLTIAKNPVGISSHIEKLNSALDLETNDVRMIG 214

Query: 181 IYGLGGIGKTTIAKAIYNCIANLFEACSFLPTIKEKCEFSRDNELTKLQESLLQDLLLIK 240
           IYGLGGIGKTT+AKA+YNCIA  FE CSFLP IKEKC FSRD+ELTKLQESLLQD+LLIK
Sbjct: 215 IYGLGGIGKTTVAKAVYNCIAYRFEGCSFLPNIKEKCNFSRDDELTKLQESLLQDILLIK 274

Query: 241 MRRSINFSDEGSNILRDRLRKKKVLVVLDDVDHSNQLEKLVGHLSWFGLGSRIIITTRNL 300
           M RSI+FSDEG                       NQ EKLVGHLSWFG GSRIIITTR+ 
Sbjct: 275 MHRSISFSDEG-----------------------NQQEKLVGHLSWFGSGSRIIITTRDF 334

Query: 301 HLLNKHNIKWTYKVDKLNHREALELFCWNAFNNSVPDVSLEELSNVAVEHADGLPLALAV 360
           HLLNK NIKWTYKVDKLNHREALELFCWNAFNN VPDVS EELSNVAVE A+GLPLAL V
Sbjct: 335 HLLNKLNIKWTYKVDKLNHREALELFCWNAFNNPVPDVSFEELSNVAVEQANGLPLALVV 394

Query: 361 VGASLCDCIKIEWECQLKKLKKIPNQDVHNKLKLSFDGLGDLEKAIFLDIACFFRGYEKE 420
           VG SLC+C K+EWE QLKKLKKIPNQDVHNKLKLSFD LGDL+K IFLDIACFFRGY  E
Sbjct: 395 VGGSLCNCSKLEWESQLKKLKKIPNQDVHNKLKLSFDRLGDLQKTIFLDIACFFRGYPTE 454

Query: 421 DVYSFFDACDFYPHSGIELLIKKSLIYIDYNKLQMHDLIQLMGREIVRQESPLDLGRRSR 427
           DVYSF DACDFY  S IE+LIKK LIYI+YN+ QMHDLIQLMG+EIVRQESP DLGRRSR
Sbjct: 455 DVYSFIDACDFYTRSEIEVLIKKCLIYIEYNRFQMHDLIQLMGKEIVRQESPSDLGRRSR 506

BLAST of Lsi10G002530 vs. TAIR 10
Match: AT5G17680.1 (disease resistance protein (TIR-NBS-LRR class), putative )

HSP 1 Score: 337.8 bits (865), Expect = 1.4e-92
Identity = 209/523 (39.96%), Postives = 290/523 (55.45%), Query Frame = 0

Query: 2   QVGINTFRDTEELNRGMQISQELCEAINGSRFFIVVFSENYASSSWCLDEIVQIMSCMEC 61
           ++GI  FRD  +L RG  IS EL +AI GSRF IVV S NYA+SSWCLDE+++I   MEC
Sbjct: 43  RMGIKAFRDDLDLQRGKSISPELIDAIKGSRFAIVVVSRNYAASSWCLDELLKI---MEC 102

Query: 62  GKGQMVWPVFYHVDPSEVRKQNGIFGEAFIVHQQ-----RFKEDLKR------------- 121
            K  +V P+FY VDPS+VR+Q G FGE    H       ++KE LK+             
Sbjct: 103 NKDTIV-PIFYEVDPSDVRRQRGSFGEDVESHSDKEKVGKWKEALKKLAAISGEDSRNWD 162

Query: 122 ---------------------------------------------SDVRMIGIYGLGGIG 181
                                                         DVRM+GI+G+GG+G
Sbjct: 163 DSKLIKKIVKDISDKLVSTSWDDSKGLIGMSSHMDFLQSMISIVDKDVRMLGIWGMGGVG 222

Query: 182 KTTIAKAIYNCIANLFEACSFLPTIKEKCEFSRDNELTKLQESLLQDLLLIKMRRSINFS 241
           KTTIAK +YN ++  F+   F+  +KE C       + +LQ   L  +   + + + + S
Sbjct: 223 KTTIAKYLYNQLSGQFQVHCFMENVKEVC---NRYGVRRLQVEFLCRMFQERDKEAWS-S 282

Query: 242 DEGSNILRDRLRKKKVLVVLDDVDHSNQLEKLVGHLSWFGLGSRIIITTRNLHLLNKHNI 301
               NI+++R R K V +VLDDVD S QL +LV    WFG GSRII+TTR+ HLL  H I
Sbjct: 283 VSCCNIIKERFRHKMVFIVLDDVDRSEQLNELVKETGWFGPGSRIIVTTRDRHLLLSHGI 342

Query: 302 KWTYKVDKLNHREALELFCWNAFNNS-VPDVSLEELSNVAVEHADGLPLALAVVGASLCD 361
              YKV  L  +EAL+LFC  AF    +     EELS  AV +A GLPLAL V+G+ L  
Sbjct: 343 NLVYKVKCLPKKEALQLFCNYAFREEIILPHGFEELSVQAVNYASGLPLALRVLGSFLYR 402

Query: 362 CIKIEWECQLKKLKKIPNQDVHNKLKLSFDGLGDLEKAIFLDIACFFRGYEKEDVYSFFD 421
             +IEWE  L +LK  P+ D+   L++S+DGL + EKAIFL I+CF+   + + V    D
Sbjct: 403 RSQIEWESTLARLKTYPHSDIMEVLRVSYDGLDEQEKAIFLYISCFYNMKQVDYVRKLLD 462

Query: 422 ACDFYPHSGIELLIKKSLIYIDYNKLQMHDLIQLMGREIVRQESPLDLGRRSRLWYHQDV 461
            C +    GI +L +KSLI      +++HDL++ MGRE+VRQ++  +  +R  LW  +D+
Sbjct: 463 LCGYAAEIGITILTEKSLIVESNGCVKIHDLLEQMGRELVRQQAVNNPAQRLLLWDPEDI 522

BLAST of Lsi10G002530 vs. TAIR 10
Match: AT5G36930.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 332.0 bits (850), Expect = 7.7e-91
Identity = 206/527 (39.09%), Postives = 287/527 (54.46%), Query Frame = 0

Query: 4   GINTFRDTEELNRGMQISQELCEAINGSRFFIVVFSENYASSSWCLDEIVQIMSCMECGK 63
           GI+TF D  EL RG  IS EL  AI  S+  IVV +++YASS+WCLDE+V IM   +   
Sbjct: 41  GISTFMDDVELQRGEYISPELLNAIETSKILIVVLTKDYASSAWCLDELVHIMKSHKNNP 100

Query: 64  GQMVWPVFYHVDPSEVRKQNGIFGEAFIVHQQRF-------------------------- 123
             MV+P+F +VDPS++R Q G + ++F  H+                             
Sbjct: 101 SHMVFPIFLYVDPSDIRWQQGSYAKSFSKHKNSHPLNKLKDWREALTKVANISGWDIKNR 160

Query: 124 ----------KEDLKR------------------------------SDVRMIGIYGLGGI 183
                     +E LKR                                VR+I IYG+GGI
Sbjct: 161 NEAECIADITREILKRLPCQYLHVPSYAVGLRSRLQHISSLLSIGSDGVRVIVIYGMGGI 220

Query: 184 GKTTIAKAIYNCIANLFEACSFLPTIKEKCEFSRDNE-LTKLQESLLQDLLLIKMRRSIN 243
           GKTT+AK  +N  ++LFE  SFL   +   E+S+  E  T LQ  LL D+L    R  I 
Sbjct: 221 GKTTLAKVAFNEFSHLFEGSSFLENFR---EYSKKPEGRTHLQHQLLSDIL---RRNDIE 280

Query: 244 FSDEGSNILRDRLRKKKVLVVLDDVDHSNQLEKLVGHLSWFGLGSRIIITTRNLHLLNKH 303
           F     + +++R R K+VL+V+DDVD  +QL         FG GSRIIITTRN+HLL + 
Sbjct: 281 FKGL-DHAVKERFRSKRVLLVVDDVDDVHQLNSAAIDRDCFGHGSRIIITTRNMHLLKQL 340

Query: 304 NIKWTYKVDKLNHREALELFCWNAFNNSVPDVSLEELSNVAVEHADGLPLALAVVGASLC 363
             + +Y   +L+  E+LELF W+AF  S P     + S   V +  GLPLA+ V+GA L 
Sbjct: 341 RAEGSYSPKELDGDESLELFSWHAFRTSEPPKEFLQHSEEVVTYCAGLPLAVEVLGAFLI 400

Query: 364 DCIKIEWECQLKKLKKIPNQDVHNKLKLSFDGLGDLEKAIFLDIACFFRGYEKEDVYSFF 423
           +    EWE  LK LK+IPN ++  KL++SF+ L   +K +FLDIACFF G +   V    
Sbjct: 401 ERSIREWESTLKLLKRIPNDNIQAKLQISFNALTIEQKDVFLDIACFFIGVDSYYVACIL 460

Query: 424 DACDFYPHSGIELLIKKSLIYIDYNKLQMHDLIQLMGREIVRQESPLDLGRRSRLWYHQD 464
           D C+ YP   + LL+++ LI I  N + MHDL++ MGR+IVR+ SP   G RSRLW H D
Sbjct: 461 DGCNLYPDIVLSLLMERCLITISGNNIMMHDLLRDMGRQIVREISPKKCGERSRLWSHND 520

BLAST of Lsi10G002530 vs. TAIR 10
Match: AT5G36930.2 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 332.0 bits (850), Expect = 7.7e-91
Identity = 206/527 (39.09%), Postives = 287/527 (54.46%), Query Frame = 0

Query: 4   GINTFRDTEELNRGMQISQELCEAINGSRFFIVVFSENYASSSWCLDEIVQIMSCMECGK 63
           GI+TF D  EL RG  IS EL  AI  S+  IVV +++YASS+WCLDE+V IM   +   
Sbjct: 44  GISTFMDDVELQRGEYISPELLNAIETSKILIVVLTKDYASSAWCLDELVHIMKSHKNNP 103

Query: 64  GQMVWPVFYHVDPSEVRKQNGIFGEAFIVHQQRF-------------------------- 123
             MV+P+F +VDPS++R Q G + ++F  H+                             
Sbjct: 104 SHMVFPIFLYVDPSDIRWQQGSYAKSFSKHKNSHPLNKLKDWREALTKVANISGWDIKNR 163

Query: 124 ----------KEDLKR------------------------------SDVRMIGIYGLGGI 183
                     +E LKR                                VR+I IYG+GGI
Sbjct: 164 NEAECIADITREILKRLPCQYLHVPSYAVGLRSRLQHISSLLSIGSDGVRVIVIYGMGGI 223

Query: 184 GKTTIAKAIYNCIANLFEACSFLPTIKEKCEFSRDNE-LTKLQESLLQDLLLIKMRRSIN 243
           GKTT+AK  +N  ++LFE  SFL   +   E+S+  E  T LQ  LL D+L    R  I 
Sbjct: 224 GKTTLAKVAFNEFSHLFEGSSFLENFR---EYSKKPEGRTHLQHQLLSDIL---RRNDIE 283

Query: 244 FSDEGSNILRDRLRKKKVLVVLDDVDHSNQLEKLVGHLSWFGLGSRIIITTRNLHLLNKH 303
           F     + +++R R K+VL+V+DDVD  +QL         FG GSRIIITTRN+HLL + 
Sbjct: 284 FKGL-DHAVKERFRSKRVLLVVDDVDDVHQLNSAAIDRDCFGHGSRIIITTRNMHLLKQL 343

Query: 304 NIKWTYKVDKLNHREALELFCWNAFNNSVPDVSLEELSNVAVEHADGLPLALAVVGASLC 363
             + +Y   +L+  E+LELF W+AF  S P     + S   V +  GLPLA+ V+GA L 
Sbjct: 344 RAEGSYSPKELDGDESLELFSWHAFRTSEPPKEFLQHSEEVVTYCAGLPLAVEVLGAFLI 403

Query: 364 DCIKIEWECQLKKLKKIPNQDVHNKLKLSFDGLGDLEKAIFLDIACFFRGYEKEDVYSFF 423
           +    EWE  LK LK+IPN ++  KL++SF+ L   +K +FLDIACFF G +   V    
Sbjct: 404 ERSIREWESTLKLLKRIPNDNIQAKLQISFNALTIEQKDVFLDIACFFIGVDSYYVACIL 463

Query: 424 DACDFYPHSGIELLIKKSLIYIDYNKLQMHDLIQLMGREIVRQESPLDLGRRSRLWYHQD 464
           D C+ YP   + LL+++ LI I  N + MHDL++ MGR+IVR+ SP   G RSRLW H D
Sbjct: 464 DGCNLYPDIVLSLLMERCLITISGNNIMMHDLLRDMGRQIVREISPKKCGERSRLWSHND 523

BLAST of Lsi10G002530 vs. TAIR 10
Match: AT5G44510.1 (target of AVRB operation1 )

HSP 1 Score: 278.9 bits (712), Expect = 7.7e-75
Identity = 186/526 (35.36%), Postives = 267/526 (50.76%), Query Frame = 0

Query: 4   GINTFRDTEELNRGMQISQELCEAINGSRFFIVVFSENYASSSWCLDEIVQIMSCMECGK 63
           GI  F D  E+ RG  I  EL +AI GS+  I++ S NY SS WCLDE+V+IM C E   
Sbjct: 67  GITPFID-NEMKRGGSIGPELLQAIRGSKIAIILLSRNYGSSKWCLDELVEIMKCRE-EL 126

Query: 64  GQMVWPVFYHVDPSEVRKQNGIFGEAF--------IVHQQRFKE---------------- 123
           GQ V  VFY VDPS+VRKQ G FG+ F            QR+K+                
Sbjct: 127 GQTVMTVFYDVDPSDVRKQKGDFGKVFKKTCVGRPEEMVQRWKQALTSAANILGEDSRNW 186

Query: 124 -------------------------------------------DLKRSDVRMIGIYGLGG 183
                                                       L   +VRMIGI+G  G
Sbjct: 187 ENEADMIIKISKDVSDVLSFTPSKDFDEFVGIEAHTTEITSLLQLDLEEVRMIGIWGPAG 246

Query: 184 IGKTTIAKAIYNCIANLFEACSFLPTIKEKC-EFSRDNELTKLQESLLQDLLLIKMRRSI 243
           IGKTTI++ +YN + + F+  + +  IK +      D    KLQ   LQ  LL +M    
Sbjct: 247 IGKTTISRVLYNKLFHQFQLGAIIDNIKVRYPRPCHDEYSAKLQ---LQKELLSQMINQK 306

Query: 244 NFSDEGSNILRDRLRKKKVLVVLDDVDHSNQLEKLVGHLSWFGLGSRIIITTRNLHLLNK 303
           +       + ++RL+ KKVL+VLDDVD   QL+ +   + WFGLGSRII+ T++L LL  
Sbjct: 307 DMVVPHLGVAQERLKDKKVLLVLDDVDGLVQLDAMAKDVQWFGLGSRIIVVTQDLKLLKA 366

Query: 304 HNIKWTYKVDKLNHREALELFCWNAFNNSVPDVSLEELSNVAVEHADGLPLALAVVGASL 363
           H IK+ YKVD     EALE+FC  AF    P V  E+++      A  LPL L V+G+ L
Sbjct: 367 HGIKYIYKVDFPTSDEALEIFCMYAFGEKSPKVGFEQIARTVTTLAGKLPLGLRVMGSYL 426

Query: 364 CDCIKIEWECQLKKLKKIPNQDVHNKLKLSFDGLGDLEKAIFLDIACFFRGYEKEDVYSF 423
               K EW   + +L+   + D+ + LK S++ L + EK +FL I CFFR    E +  F
Sbjct: 427 RRMSKQEWAKSIPRLRTSLDDDIESVLKFSYNSLAEQEKDLFLHITCFFRRERIETLEVF 486

Query: 424 FDACDFYPHSGIELLIKKSLIYIDYNKLQMHDLIQLMGREIVRQESPLDLGRRSRLWYHQ 461
                     G+++L  KSL+ ++   ++MH+L+  +G +IVR++S    G+R  L   +
Sbjct: 487 LAKKSVDMRQGLQILADKSLLSLNLGNIEMHNLLVQLGLDIVRKQSIHKPGKRQFLVDTE 546

BLAST of Lsi10G002530 vs. TAIR 10
Match: AT5G41750.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 272.3 bits (695), Expect = 7.2e-73
Identity = 187/539 (34.69%), Postives = 280/539 (51.95%), Query Frame = 0

Query: 4   GINTFRDTEELNRGMQISQELCEAINGSRFFIVVFSENYASSSWCLDEIVQIMSCMECGK 63
           GI TF D ++++RG  I  EL + I  +R  IVV S+ YASSSWCLDE+V+I+ C E   
Sbjct: 40  GITTFND-QKIDRGQTIGPELIQGIREARVSIVVLSKKYASSSWCLDELVEILKCKE-AL 99

Query: 64  GQMVWPVFYHVDPSEVRKQNGIFGEAFIVHQQRFKEDLK--------------------- 123
           GQ+V  VFY VDPS+V+KQ+G+FGEAF    Q   E++K                     
Sbjct: 100 GQIVMTVFYEVDPSDVKKQSGVFGEAFEKTCQGKNEEVKIRWRNALAHVATIAGEHSLNW 159

Query: 124 ----------------------------------------------RSDVRMIGIYGLGG 183
                                                           +V+MIGI+G  G
Sbjct: 160 DNEAKMIQKIVTDVSDKLNLTPSRDFEGMVGMEAHLKRLNSLLCLESDEVKMIGIWGPAG 219

Query: 184 IGKTTIAKAIYNCIANLFEACSFLPTIKEKCEFSRDNELTKLQESLLQDLLLIKMRRSIN 243
           IGKTTIA+ ++N I+++F    F+  +K   +   ++  +KL    LQ  LL ++ +  N
Sbjct: 220 IGKTTIARTLFNKISSIFPFKCFMENLKGSIKGGAEH-YSKLS---LQKQLLSEILKQEN 279

Query: 244 FSDEGSNILRDRLRKKKVLVVLDDVDHSNQLEKLVGHLSWFGLGSRIIITTRNLHLLNKH 303
                   ++  L  +KVL++LDDVD   QLE L    SWFG GSRII+TT + ++L  H
Sbjct: 280 MKIHHLGTIKQWLHDQKVLIILDDVDDLEQLEVLAEDPSWFGSGSRIIVTTEDKNILKAH 339

Query: 304 NIKWTYKVDKLNHREALELFCWNAF-NNSVPDVSLEELSNVAVEHADGLPLALAVVGASL 363
            I+  Y VD  +  EALE+ C +AF  +S+PD   EEL+N   E    LPL L VVGASL
Sbjct: 340 RIQDIYHVDFPSEEEALEILCLSAFKQSSIPD-GFEELANKVAELCGNLPLGLCVVGASL 399

Query: 364 CDCIKIEWECQLKKLKKIPNQDVHNKLKLSFDGLGDLEKAIFLDIACFFRGYEKEDVYSF 423
               K EWE  L +++   ++++ N L++ +D L   ++++FL IACFF   + + + + 
Sbjct: 400 RRKSKNEWERLLSRIESSLDKNIDNILRIGYDRLSTEDQSLFLHIACFFNNEKVDYLTAL 459

Query: 424 FDACDFYPHSGIELLIKKSLIYI--DYNKLQMHDLIQLMGREIVRQESPLDLGRRSRLWY 472
                    +G  +L  +SL+ I  D + +  H L+Q +GR IV ++ P + G+R  L  
Sbjct: 460 LADRKLDVVNGFNILADRSLVRISTDGHVVMHHYLLQKLGRRIVHEQWPNEPGKRQFLIE 519

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
V9M3981.6e-10141.35Disease resistance protein RUN1 OS=Vitis rotundifolia OX=103349 GN=RUN1 PE=1 SV=... [more]
V9M2S52.2e-9840.87Disease resistance protein RPV1 OS=Vitis rotundifolia OX=103349 GN=RPV1 PE=1 SV=... [more]
A0A290U7C42.2e-9038.99Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV... [more]
Q403925.2e-8438.26TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1[more]
Q9FI141.1e-7335.36Disease resistance protein TAO1 OS=Arabidopsis thaliana OX=3702 GN=TAO1 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
A0A5D3C5B52.6e-22773.62TMV resistance protein N-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A1S4E3272.6e-22773.62TMV resistance protein N-like OS=Cucumis melo OX=3656 GN=LOC103498360 PE=4 SV=1[more]
A0A5A7TN331.7e-18668.55TMV resistance protein N-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sc... [more]
A0A6J1D4F54.5e-16358.38TMV resistance protein N-like OS=Momordica charantia OX=3673 GN=LOC111017198 PE=... [more]
A0A6J1D3931.9e-13456.83TMV resistance protein N-like OS=Momordica charantia OX=3673 GN=LOC111017199 PE=... [more]
Match NameE-valueIdentityDescription
XP_038901313.11.8e-22774.35disease resistance protein RUN1-like [Benincasa hispida][more]
TYK06530.15.3e-22773.62TMV resistance protein N-like [Cucumis melo var. makuwa][more]
XP_016902380.15.3e-22773.62PREDICTED: TMV resistance protein N-like [Cucumis melo][more]
XP_038902439.17.6e-22674.26disease resistance protein RUN1-like [Benincasa hispida][more]
KAA0042991.13.5e-18668.55TMV resistance protein N-like [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT5G17680.11.4e-9239.96disease resistance protein (TIR-NBS-LRR class), putative [more]
AT5G36930.17.7e-9139.09Disease resistance protein (TIR-NBS-LRR class) family [more]
AT5G36930.27.7e-9139.09Disease resistance protein (TIR-NBS-LRR class) family [more]
AT5G44510.17.7e-7535.36target of AVRB operation1 [more]
AT5G41750.17.2e-7334.69Disease resistance protein (TIR-NBS-LRR class) family [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00364DISEASERSISTcoord: 452..468
score: 19.41
coord: 108..123
score: 60.47
coord: 188..202
score: 56.5
coord: 281..295
score: 50.5
NoneNo IPR availablePANTHERPTHR11017:SF422RESISTANCE PROTEIN (TIR-NBS-LRR CLASS), PUTATIVE-RELATEDcoord: 2..103
coord: 105..465
IPR000157Toll/interleukin-1 receptor homology (TIR) domainSMARTSM00255till_3coord: 4..117
e-value: 6.2E-10
score: 49.0
IPR000157Toll/interleukin-1 receptor homology (TIR) domainPFAMPF01582TIRcoord: 2..100
e-value: 7.4E-30
score: 104.0
IPR000157Toll/interleukin-1 receptor homology (TIR) domainPROSITEPS50104TIRcoord: 1..109
score: 23.935143
IPR035897Toll/interleukin-1 receptor homology (TIR) domain superfamilyGENE3D3.40.50.10140coord: 1..93
e-value: 1.6E-18
score: 68.6
IPR035897Toll/interleukin-1 receptor homology (TIR) domain superfamilySUPERFAMILY52200Toll/Interleukin receptor TIR domaincoord: 3..94
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 94..240
e-value: 1.4E-27
score: 98.3
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 96..340
IPR042197Apoptotic protease-activating factors, helical domainGENE3D1.10.8.430coord: 246..330
e-value: 2.7E-15
score: 57.7
IPR002182NB-ARCPFAMPF00931NB-ARCcoord: 96..313
e-value: 1.2E-29
score: 103.3
IPR044974Disease resistance protein, plantsPANTHERPTHR11017LEUCINE-RICH REPEAT-CONTAINING PROTEINcoord: 2..103
coord: 105..465
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 318..422

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi10G002530.1Lsi10G002530.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
biological_process GO:0007165 signal transduction
molecular_function GO:0043531 ADP binding
molecular_function GO:0003953 NAD+ nucleosidase activity