Lsi10G001210 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi10G001210
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionMembrane metalloprotease ARASP, chloroplastic
Locationchr10: 1874915 .. 1876288 (-)
RNA-Seq ExpressionLsi10G001210
SyntenyLsi10G001210
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTAGTAAATCTCTCTTCCTCTTCTTTTTCTGTCATTAAATTAGCGAATTTAAAGTCCCCCTTCTCTGATTTTTCAGTCAAGTCCAGAACCCACTTCTCTAAATCCCCATCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCATTATGTCATTCTTTCCCGTCCTCGGACCTCAGTTTCAAGAACAGACACCAAATTTTCTGGGACAAGGCCAAAATCCCATGTCGGAGGAATGGCGATTTCAGGCTGTGGGCTTTTGCCGGAATCGATATTGGAAGTGCTCAGTCGGTACTGGAGGCGGCGGCGGTGTTGACGGCTATCATTGTTGTTCATGAGAGTGGACACTTCCTCGCCGCCTGTCTACAGGGCATCCATGTAAGTAAATTTGCAATCGGTTTTGGTCCAATTCTTGCAAAATTCAACAAAAACAATGTAGAATACTCCATTAGGGCTTTTCCTCTCGGTGGATTTGTGGGTTTTCCTGATAATGATCCTGAAAGCGATATTCCAGTGGATGATGAAAATTTGCTTAAAAATAGACCGATATTGGATAGAGTGTTAGTGATTTCTGCTGGTGTTATTGCCAATATAATCTTTGCATACATAATTATCTTAGTTCAAGTCTCGTCCGTTGGTTTGCCTGTTCAAGAGCCCTTTCCAGGCGTTCTTGTGCCGGAGGTTCGAACTCTTTCGGCTGCTTCTCGTGATGGGTTGCTTCCAGGGGATGTGATTCTTGCTGTTAATGGAAATGAACTGCCAAAATTGGGTTCTACAGCAGTTTCTGATCTCGTTGAAGCAATTAAAAGAAGCCCCAATAGAACTGTGCTTCTCAAAGTTGAAAGAGGGAAACAGGATCTTGAAATTGGGGTCACCCCAGATGAGAGTTTTGATGGAACAGGGAGAATTGGGGTTCAGCTTTCACCTAATGTAAAGATTTCAAAAGTTGTAGCAAAGAATTTCTTGGAGGCTTTTAACTACTCTAGAAAGGAATTTTTGGGTCTTTCATATAATGTTCTGGATAGCTTAAAACAAACTTTTCTGAATTTCTCTCAGTCAGCTAGTAAGGTTTCTGGTCCTGTGGCCATTATTGCTGTTGGTGCAGAAGTTGCGAGGTCCAACATTGATGGGCTCTATCAATTTGCAGCTGTGCTTAACATTAACCTTGCTGTTATAAACCTTTTGCCCTTGCCTGCTTTGGATGGTGGTTCGTTGGCTTTGATCCTCATTGAGGCTGTAAGGGGAGGGAGGAAGTTGCCTCTAGAACTCGAGCAGCGAATTATGTCATCTGGGGTCATGTTCGTTGTACTTCTCGGGCTATTCCTTATCATCCGAGACACGTTAAACCTTGAATTTATAAAGGACTTGTTATGA

mRNA sequence

ATGTTAGTAAATCTCTCTTCCTCTTCTTTTTCTGTCATTAAATTAGCGAATTTAAAGTCCCCCTTCTCTGATTTTTCAGTCAAGTCCAGAACCCACTTCTCTAAATCCCCATCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCATTATGTCATTCTTTCCCGTCCTCGGACCTCAGTTTCAAGAACAGACACCAAATTTTCTGGGACAAGGCCAAAATCCCATGTCGGAGGAATGGCGATTTCAGGCTGTGGGCTTTTGCCGGAATCGATATTGGAAGTGCTCAGTCGGTACTGGAGGCGGCGGCGGTGTTGACGGCTATCATTGTTGTTCATGAGAGTGGACACTTCCTCGCCGCCTGTCTACAGGGCATCCATGTAAGTAAATTTGCAATCGGTTTTGGTCCAATTCTTGCAAAATTCAACAAAAACAATGTAGAATACTCCATTAGGGCTTTTCCTCTCGGTGGATTTGTGGGTTTTCCTGATAATGATCCTGAAAGCGATATTCCAGTGGATGATGAAAATTTGCTTAAAAATAGACCGATATTGGATAGAGTGTTAGTGATTTCTGCTGGTGTTATTGCCAATATAATCTTTGCATACATAATTATCTTAGTTCAAGTCTCGTCCGTTGGTTTGCCTGTTCAAGAGCCCTTTCCAGGCGTTCTTGTGCCGGAGGTTCGAACTCTTTCGGCTGCTTCTCGTGATGGGTTGCTTCCAGGGGATGTGATTCTTGCTGTTAATGGAAATGAACTGCCAAAATTGGGTTCTACAGCAGTTTCTGATCTCGTTGAAGCAATTAAAAGAAGCCCCAATAGAACTGTGCTTCTCAAAGTTGAAAGAGGGAAACAGGATCTTGAAATTGGGGTCACCCCAGATGAGAGTTTTGATGGAACAGGGAGAATTGGGGTTCAGCTTTCACCTAATGTAAAGATTTCAAAAGTTGTAGCAAAGAATTTCTTGGAGGCTTTTAACTACTCTAGAAAGGAATTTTTGGGTCTTTCATATAATGTTCTGGATAGCTTAAAACAAACTTTTCTGAATTTCTCTCAGTCAGCTAGTAAGGTTTCTGGTCCTGTGGCCATTATTGCTGTTGGTGCAGAAGTTGCGAGGTCCAACATTGATGGGCTCTATCAATTTGCAGCTGTGCTTAACATTAACCTTGCTGTTATAAACCTTTTGCCCTTGCCTGCTTTGGATGGTGGTTCGTTGGCTTTGATCCTCATTGAGGCTGTAAGGGGAGGGAGGAAGTTGCCTCTAGAACTCGAGCAGCGAATTATGTCATCTGGGGTCATGTTCGTTGTACTTCTCGGGCTATTCCTTATCATCCGAGACACGTTAAACCTTGAATTTATAAAGGACTTGTTATGA

Coding sequence (CDS)

ATGTTAGTAAATCTCTCTTCCTCTTCTTTTTCTGTCATTAAATTAGCGAATTTAAAGTCCCCCTTCTCTGATTTTTCAGTCAAGTCCAGAACCCACTTCTCTAAATCCCCATCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCATTATGTCATTCTTTCCCGTCCTCGGACCTCAGTTTCAAGAACAGACACCAAATTTTCTGGGACAAGGCCAAAATCCCATGTCGGAGGAATGGCGATTTCAGGCTGTGGGCTTTTGCCGGAATCGATATTGGAAGTGCTCAGTCGGTACTGGAGGCGGCGGCGGTGTTGACGGCTATCATTGTTGTTCATGAGAGTGGACACTTCCTCGCCGCCTGTCTACAGGGCATCCATGTAAGTAAATTTGCAATCGGTTTTGGTCCAATTCTTGCAAAATTCAACAAAAACAATGTAGAATACTCCATTAGGGCTTTTCCTCTCGGTGGATTTGTGGGTTTTCCTGATAATGATCCTGAAAGCGATATTCCAGTGGATGATGAAAATTTGCTTAAAAATAGACCGATATTGGATAGAGTGTTAGTGATTTCTGCTGGTGTTATTGCCAATATAATCTTTGCATACATAATTATCTTAGTTCAAGTCTCGTCCGTTGGTTTGCCTGTTCAAGAGCCCTTTCCAGGCGTTCTTGTGCCGGAGGTTCGAACTCTTTCGGCTGCTTCTCGTGATGGGTTGCTTCCAGGGGATGTGATTCTTGCTGTTAATGGAAATGAACTGCCAAAATTGGGTTCTACAGCAGTTTCTGATCTCGTTGAAGCAATTAAAAGAAGCCCCAATAGAACTGTGCTTCTCAAAGTTGAAAGAGGGAAACAGGATCTTGAAATTGGGGTCACCCCAGATGAGAGTTTTGATGGAACAGGGAGAATTGGGGTTCAGCTTTCACCTAATGTAAAGATTTCAAAAGTTGTAGCAAAGAATTTCTTGGAGGCTTTTAACTACTCTAGAAAGGAATTTTTGGGTCTTTCATATAATGTTCTGGATAGCTTAAAACAAACTTTTCTGAATTTCTCTCAGTCAGCTAGTAAGGTTTCTGGTCCTGTGGCCATTATTGCTGTTGGTGCAGAAGTTGCGAGGTCCAACATTGATGGGCTCTATCAATTTGCAGCTGTGCTTAACATTAACCTTGCTGTTATAAACCTTTTGCCCTTGCCTGCTTTGGATGGTGGTTCGTTGGCTTTGATCCTCATTGAGGCTGTAAGGGGAGGGAGGAAGTTGCCTCTAGAACTCGAGCAGCGAATTATGTCATCTGGGGTCATGTTCGTTGTACTTCTCGGGCTATTCCTTATCATCCGAGACACGTTAAACCTTGAATTTATAAAGGACTTGTTATGA

Protein sequence

MLVNLSSSSFSVIKLANLKSPFSDFSVKSRTHFSKSPSSSSSSSSSSSSLCHSFPSSDLSFKNRHQIFWDKAKIPCRRNGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPESDIPVDDENLLKNRPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGKQDLEIGVTPDESFDGTGRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFLNFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAVRGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL
Homology
BLAST of Lsi10G001210 vs. ExPASy Swiss-Prot
Match: O80885 (Membrane metalloprotease ARASP, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ARASP PE=2 SV=1)

HSP 1 Score: 569.7 bits (1467), Expect = 3.0e-161
Identity = 312/464 (67.24%), Postives = 370/464 (79.74%), Query Frame = 0

Query: 1   MLVNLSSSSFSVIK---LANLKSPFSDFSVKSRTHFSKSPSSSSSSSSSSSSLCHSFPS- 60
           ML+N+SSS  S      L+N  +P S F  +S+TH SKS               H FP  
Sbjct: 1   MLLNISSSPISHRNPHFLSNFNNPISYFPRRSKTHLSKS---------------HFFPKF 60

Query: 61  ---SDLSFKNRHQIFWDKAKIPCRRNGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHE 120
              S+ S KNR  + +   + P     DFR  A +GID+GS +SVLEA AVLT IIVVHE
Sbjct: 61  TPLSNQSLKNR--VLFGNKRYPDGERFDFRSRAISGIDLGSFESVLEAIAVLTTIIVVHE 120

Query: 121 SGHFLAACLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPESDIPVD 180
           SGHFLAA LQGIHVSKFAIGFGPILAKF+ NNVEYS+RAFPLGGFVGFPDNDP+S+IP+D
Sbjct: 121 SGHFLAASLQGIHVSKFAIGFGPILAKFDYNNVEYSLRAFPLGGFVGFPDNDPDSEIPID 180

Query: 181 DENLLKNRPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEVRTLSA 240
           DENLLKNRP LDR +V+SAG+IAN+IFAY II VQV SVGLPVQE FPGVLVPEV+T SA
Sbjct: 181 DENLLKNRPTLDRSIVVSAGIIANVIFAYAIIFVQVLSVGLPVQEAFPGVLVPEVKTFSA 240

Query: 241 ASRDGLLPGDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGKQDLEIGVTP 300
           ASRDGLL GDVILAV+G EL K G  AVS +V+ +KR+P   V+ ++ERG +D +I VTP
Sbjct: 241 ASRDGLLSGDVILAVDGTELSKTGPDAVSKIVDIVKRNPKSNVVFRIERGGEDFDIRVTP 300

Query: 301 DESFDGTGRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFLNFSQS 360
           D++FDGTG+IGVQLSPNV+I+KV  +N  E F +  +EF+GLS NVLD LKQTF NFSQ+
Sbjct: 301 DKNFDGTGKIGVQLSPNVRITKVRPRNIPETFRFVGREFMGLSSNVLDGLKQTFFNFSQT 360

Query: 361 ASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAV 420
           ASKV+GPVAIIAVGAEVARSNIDGLYQFAA+LNINLAVINLLPLPALDGG+LALIL+EAV
Sbjct: 361 ASKVAGPVAIIAVGAEVARSNIDGLYQFAALLNINLAVINLLPLPALDGGTLALILLEAV 420

Query: 421 RGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 458
           RGG+KLP+E+EQ IMSSG+M V+ LGLFLI++DTL+L+FIK++L
Sbjct: 421 RGGKKLPVEVEQGIMSSGIMLVIFLGLFLIVKDTLSLDFIKEML 447

BLAST of Lsi10G001210 vs. ExPASy Swiss-Prot
Match: O23053 (Probable membrane metalloprotease ARASP2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ARASP2 PE=2 SV=1)

HSP 1 Score: 562.8 bits (1449), Expect = 3.6e-159
Identity = 312/460 (67.83%), Postives = 369/460 (80.22%), Query Frame = 0

Query: 1   MLVNLSSSSFS---VIKLANLKSPFSDFSVKSRTHFSKSPSSSSSSSSSSSSLCHSFPSS 60
           ML+N+SSS  S      L++  +P S+F  KS+TH  KS  S+ S+ S   +   +F   
Sbjct: 1   MLLNISSSPISHRIPHFLSDFNNPTSNFPPKSKTHLPKSNLSTLSNHSLYGTKNRAF--- 60

Query: 61  DLSFKNRHQIFWDKAKIPCRRNGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHF 120
              +KN             +RN   R  A    D GS +SVLEA+AVLTAIIVVHE+GHF
Sbjct: 61  ---YKN-------------KRNPYNRTQALGRFDFGSLESVLEASAVLTAIIVVHETGHF 120

Query: 121 LAACLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPESDIPVDDENL 180
           LAA LQGI VSKFAIGFGPILAKFN NNVEYS+RAFPLGGFVGFPDNDP+SDIPVDD NL
Sbjct: 121 LAASLQGIRVSKFAIGFGPILAKFNSNNVEYSLRAFPLGGFVGFPDNDPDSDIPVDDRNL 180

Query: 181 LKNRPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEVRTLSAASRD 240
           LKNRPILDRV+V+SAG++AN+IFAY II  QV SVGLPVQE FPGVLVP+V++ SAASRD
Sbjct: 181 LKNRPILDRVIVVSAGIVANVIFAYAIIFTQVVSVGLPVQESFPGVLVPDVKSFSAASRD 240

Query: 241 GLLPGDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGKQDLEIGVTPDESF 300
           GLLPGDVILAV+G EL   GS +VS +V+ +KR+P   VLL++ERGK+  EI +TPD+SF
Sbjct: 241 GLLPGDVILAVDGTELSNSGSDSVSKVVDVVKRNPEHNVLLRIERGKESFEIRITPDKSF 300

Query: 301 DGTGRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFLNFSQSASKV 360
           DGTG+IGVQLSPNV+  KV  KN  E F+++ +EF GLSYNVLDSLKQTFLNFSQ+ASKV
Sbjct: 301 DGTGKIGVQLSPNVRFGKVRPKNIPETFSFAGREFFGLSYNVLDSLKQTFLNFSQTASKV 360

Query: 361 SGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAVRGGR 420
           +GPVAIIAVGAEVARSN DGLYQFAA+LN+NLAVINLLPLPALDGG+LALIL+EAVRGGR
Sbjct: 361 AGPVAIIAVGAEVARSNADGLYQFAALLNLNLAVINLLPLPALDGGTLALILLEAVRGGR 420

Query: 421 KLPLELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 458
           KLPLE+EQ IMSSG+M V+ LGLFLI++DTLNL+FIK++L
Sbjct: 421 KLPLEVEQGIMSSGIMLVLFLGLFLIVKDTLNLDFIKEML 441

BLAST of Lsi10G001210 vs. ExPASy Swiss-Prot
Match: P73714 (Putative zinc metalloprotease slr1821 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=slr1821 PE=3 SV=1)

HSP 1 Score: 331.3 bits (848), Expect = 1.8e-89
Identity = 176/364 (48.35%), Postives = 252/364 (69.23%), Query Frame = 0

Query: 94  SAQSVLEAAAVLTAIIVVHESGHFLAACLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAF 153
           S  + L A  VL  +I VHE GHF AA LQGIHV++FA+GFGP L K+     EYSIRA 
Sbjct: 2   SVLAALAAIGVLAVLIAVHELGHFAAARLQGIHVTRFALGFGPPLLKYQGAETEYSIRAI 61

Query: 154 PLGGFVGFPDNDPESDIPVDDENLLKNRPILDRVLVISAGVIANIIFAYIIILVQVSSVG 213
           PLGG+V FPD+DP+S+IP DD NLLKNRPILDR +VISAGVIAN++FAY +++ QVS++G
Sbjct: 62  PLGGYVAFPDDDPDSEIPADDPNLLKNRPILDRAIVISAGVIANLVFAYFLLIGQVSTIG 121

Query: 214 LPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSDLVEAIKRSPN 273
              Q   PG+++P+V + SAA   G+ PGD++L++ GN LP     A +  ++ ++RSP+
Sbjct: 122 F--QNIQPGLVIPQVDSASAAQVAGMEPGDIVLSLQGNTLPGF-PDATTQFIDIVRRSPS 181

Query: 274 RTVLLKVERGKQDLEIGVTPDESFDGTGRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFL 333
             + ++V+RG++   + +TP    +G G+IGV L PNV+  +  A N LEA  YS + F 
Sbjct: 182 VPITVEVQRGEETKTLTITPTPDAEGKGKIGVALLPNVETKR--ASNPLEALTYSAEAFE 241

Query: 334 GLSYNVLDSLKQTFLNFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVIN 393
            +         Q   NF+ +AS+V+GPV I+  GA +ARS+   L+QF A+++INLAVIN
Sbjct: 242 RIVKLTTQGFWQLISNFADNASQVAGPVKIVEYGANIARSDASNLFQFGALISINLAVIN 301

Query: 394 LLPLPALDGGSLALILIEAVRGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFI 453
           +LPLPALDGG L  +LIE +  G+ LP + +  +M +G++ ++ LG+FLI+RDTLNL F+
Sbjct: 302 ILPLPALDGGQLVFLLIEGLL-GKPLPEKFQMGVMQTGLVLLLSLGVFLIVRDTLNLTFV 359

Query: 454 KDLL 458
           ++ L
Sbjct: 362 QEFL 359

BLAST of Lsi10G001210 vs. ExPASy Swiss-Prot
Match: Q8YQ64 (Putative zinc metalloprotease all3971 OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=all3971 PE=3 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 2.0e-80
Identity = 172/358 (48.04%), Postives = 243/358 (67.88%), Query Frame = 0

Query: 97  SVLEAAAVLTAIIVVHESGHFLAACLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLG 156
           SVL A AVL  +I+VHE GHF+AA  QGIHV++F++GFGP+L K+     EY+IRAFPLG
Sbjct: 2   SVLAAIAVLAVLILVHELGHFVAARSQGIHVNRFSLGFGPVLWKYQGAETEYAIRAFPLG 61

Query: 157 GFVGFPDNDPESDIPVDDENLLKNRPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPV 216
           GFVGFPD+DP+SDIP +D NLL+NRPILDR +VISAGVIAN+IFAY+++L QV  VG+  
Sbjct: 62  GFVGFPDDDPDSDIPPNDPNLLRNRPILDRAIVISAGVIANLIFAYMLLLAQVGFVGIG- 121

Query: 217 QEPFPGV----LVPEVRTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSDLVEAIKRSP 276
           Q   PGV    L PEV   + A+  GL PGDVIL+ N  E        +  L + IK SP
Sbjct: 122 QASQPGVSIQQLAPEVS--AVATNAGLKPGDVILSANQKEF-GTSLQEIEALRDIIKNSP 181

Query: 277 NRTVLLKVERGKQDLEIGVTPDESFDGTGRIGVQLSPNVKISKVVAKNFLEAFNYSRKEF 336
            +++ L V RG + L + V P+    G G IG+ L+PN K+ +    +  +AF+    EF
Sbjct: 182 GKSIQLTVARGDERLSVNVIPEAKPAG-GSIGIGLAPNGKVERRPV-SLSKAFSVGASEF 241

Query: 337 LGLSYNVLDSLKQTFLNFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVI 396
             +         Q   NF ++AS+V+GP+ I+ +GA +A+++   L+ FAA+++INLAVI
Sbjct: 242 QRIVVMTFKGFGQLVTNFGETASQVAGPIKIVEIGANIAQNDTGSLFFFAALISINLAVI 301

Query: 397 NLLPLPALDGGSLALILIEAVRGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDTLNL 451
           N+LPLPALDGG LA +LIE +R G+ LP  +++ +M +G++ ++ LG+FLI+++T  L
Sbjct: 302 NILPLPALDGGQLAFLLIEGLR-GKPLPNRIQEGVMQTGLVLLLGLGIFLIVKETTQL 352

BLAST of Lsi10G001210 vs. ExPASy Swiss-Prot
Match: O31754 (Regulator of sigma-W protease RasP OS=Bacillus subtilis (strain 168) OX=224308 GN=rasP PE=1 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 2.4e-33
Identity = 127/435 (29.20%), Postives = 185/435 (42.53%), Query Frame = 0

Query: 97  SVLEAAAVLTAIIVVHESGHFLAACLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLG 156
           +V+    +   ++  HE GH L A   GI   +FAIGFGP +  F KN   Y+IR  P+G
Sbjct: 5   TVIAFIIIFGTLVFFHELGHLLLAQRAGILCREFAIGFGPKIFSFKKNETVYTIRLLPVG 64

Query: 157 GFVGFPDNDPE------------------------------------------------- 216
           GFV     DPE                                                 
Sbjct: 65  GFVRMAGEDPEMIEVKPGYTVGLLFNKEDQVEKVIINQKEKYPDALVIEVETADLEHDMK 124

Query: 217 --------------------SDIPVDDENL--------LKNRPILDRVLVISAGVIANII 276
                               S   VD E +          ++P+  R+  I+AG I N I
Sbjct: 125 ITGYEQGKEDELSSFTVSETSFFIVDGEEVQIAPYNRQFGSKPVWQRIKAIAAGPIMNFI 184

Query: 277 FAYIIILVQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAVNGNELPKLGST 336
            AY+I+++     G+P  EP  G L    R    A+  GL  GD I ++NG ++      
Sbjct: 185 LAYVILVMLGLIQGVPSNEPMLGQLTDNGR----AAEAGLKEGDYIQSINGEKM-----R 244

Query: 337 AVSDLVEAIKRSPNRTVLLKVERGKQDLEIGVTP----DESFDGTGRIGVQLSPNVKISK 396
           + +D+V A+K +P + + + V+R  + L I VTP    DE+    GR G        +  
Sbjct: 245 SWTDIVSAVKENPEKEMDVAVKRDNKTLHISVTPEAVKDENKKTIGRFGSYAPTEKGVLS 304

Query: 397 VVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFLNFSQSASKVSGPVAIIAVGAEVARSNI 451
            VA       + ++     LS  V    K   L         SGPV I  +  +VA++ I
Sbjct: 305 AVAYGATSTVDVTKAILTNLSKLVTGQFKLDML---------SGPVGIYDMTDQVAKTGI 364

BLAST of Lsi10G001210 vs. ExPASy TrEMBL
Match: A0A5D3E082 (Putative membrane metalloprotease ARASP2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold3695G00250 PE=3 SV=1)

HSP 1 Score: 817.0 bits (2109), Expect = 4.0e-233
Identity = 444/457 (97.16%), Postives = 448/457 (98.03%), Query Frame = 0

Query: 1   MLVNLSSSSFSVIKLANLKSPFSDFSVKSRTHFSKSPSSSSSSSSSSSSLCHSFPSSDLS 60
           ML+NL SSSFSVIKLANLKSPFSDFSVKSRTHFSKS SSSSSSSSSSSSL HS PSS+LS
Sbjct: 1   MLLNLPSSSFSVIKLANLKSPFSDFSVKSRTHFSKSLSSSSSSSSSSSSLWHSPPSSELS 60

Query: 61  FKNRHQIFWDKAKIPCRRNGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAA 120
           FKNRHQIFWDK KI CRRNGDFRLWAFAG+DIGSAQSVLEAAAVLTAIIVVHESGHFLAA
Sbjct: 61  FKNRHQIFWDKTKIRCRRNGDFRLWAFAGMDIGSAQSVLEAAAVLTAIIVVHESGHFLAA 120

Query: 121 CLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPESDIPVDDENLLKN 180
           CLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPESDIPVDDENLLKN
Sbjct: 121 CLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPESDIPVDDENLLKN 180

Query: 181 RPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLL 240
           RPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEV TLSAASRDGLL
Sbjct: 181 RPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEVLTLSAASRDGLL 240

Query: 241 PGDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGKQDLEIGVTPDESFDGT 300
           PGDVILAVNGNELP LGSTAVSDLVEAIKRSPNRTVLLKVERG QDLEIGVTPD+SFDGT
Sbjct: 241 PGDVILAVNGNELPNLGSTAVSDLVEAIKRSPNRTVLLKVERGNQDLEIGVTPDKSFDGT 300

Query: 301 GRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFLNFSQSASKVSGP 360
           GRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFLNFSQSASKVSGP
Sbjct: 301 GRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFLNFSQSASKVSGP 360

Query: 361 VAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAVRGGRKLP 420
           VAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAVRGGRKLP
Sbjct: 361 VAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAVRGGRKLP 420

Query: 421 LELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 458
           LELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL
Sbjct: 421 LELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 457

BLAST of Lsi10G001210 vs. ExPASy TrEMBL
Match: A0A1S3C376 (probable membrane metalloprotease ARASP2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103495966 PE=3 SV=1)

HSP 1 Score: 817.0 bits (2109), Expect = 4.0e-233
Identity = 444/457 (97.16%), Postives = 448/457 (98.03%), Query Frame = 0

Query: 1   MLVNLSSSSFSVIKLANLKSPFSDFSVKSRTHFSKSPSSSSSSSSSSSSLCHSFPSSDLS 60
           ML+NL SSSFSVIKLANLKSPFSDFSVKSRTHFSKS SSSSSSSSSSSSL HS PSS+LS
Sbjct: 1   MLLNLPSSSFSVIKLANLKSPFSDFSVKSRTHFSKSLSSSSSSSSSSSSLWHSPPSSELS 60

Query: 61  FKNRHQIFWDKAKIPCRRNGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAA 120
           FKNRHQIFWDK KI CRRNGDFRLWAFAG+DIGSAQSVLEAAAVLTAIIVVHESGHFLAA
Sbjct: 61  FKNRHQIFWDKTKIRCRRNGDFRLWAFAGMDIGSAQSVLEAAAVLTAIIVVHESGHFLAA 120

Query: 121 CLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPESDIPVDDENLLKN 180
           CLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPESDIPVDDENLLKN
Sbjct: 121 CLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPESDIPVDDENLLKN 180

Query: 181 RPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLL 240
           RPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEV TLSAASRDGLL
Sbjct: 181 RPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEVLTLSAASRDGLL 240

Query: 241 PGDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGKQDLEIGVTPDESFDGT 300
           PGDVILAVNGNELP LGSTAVSDLVEAIKRSPNRTVLLKVERG QDLEIGVTPD+SFDGT
Sbjct: 241 PGDVILAVNGNELPNLGSTAVSDLVEAIKRSPNRTVLLKVERGNQDLEIGVTPDKSFDGT 300

Query: 301 GRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFLNFSQSASKVSGP 360
           GRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFLNFSQSASKVSGP
Sbjct: 301 GRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFLNFSQSASKVSGP 360

Query: 361 VAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAVRGGRKLP 420
           VAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAVRGGRKLP
Sbjct: 361 VAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAVRGGRKLP 420

Query: 421 LELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 458
           LELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL
Sbjct: 421 LELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 457

BLAST of Lsi10G001210 vs. ExPASy TrEMBL
Match: A0A0A0LT84 (PDZ domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G417840 PE=3 SV=1)

HSP 1 Score: 811.6 bits (2095), Expect = 1.7e-231
Identity = 443/457 (96.94%), Postives = 448/457 (98.03%), Query Frame = 0

Query: 1   MLVNLSSSSFSVIKLANLKSPFSDFSVKSRTHFSKSPSSSSSSSSSSSSLCHSFPSSDLS 60
           ML+NL SSSFSVIKLANLKSPFSDFSVKSRTHFSKS  SSSSSSSSSSSL HS PSS+LS
Sbjct: 1   MLLNLPSSSFSVIKLANLKSPFSDFSVKSRTHFSKS-LSSSSSSSSSSSLWHSPPSSELS 60

Query: 61  FKNRHQIFWDKAKIPCRRNGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAA 120
           FK+RHQIFWDK KI CRRNGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAA
Sbjct: 61  FKDRHQIFWDKTKIRCRRNGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAA 120

Query: 121 CLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPESDIPVDDENLLKN 180
           CLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDP+SDIPVDDENLLKN
Sbjct: 121 CLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPVDDENLLKN 180

Query: 181 RPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLL 240
           RPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEV TLSAASRDGLL
Sbjct: 181 RPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEVLTLSAASRDGLL 240

Query: 241 PGDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGKQDLEIGVTPDESFDGT 300
           PGDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERG QDLEIGVTPD+SFDGT
Sbjct: 241 PGDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGNQDLEIGVTPDKSFDGT 300

Query: 301 GRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFLNFSQSASKVSGP 360
           GRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFLNFSQSASKVSGP
Sbjct: 301 GRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFLNFSQSASKVSGP 360

Query: 361 VAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAVRGGRKLP 420
           VAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAVRGGRKLP
Sbjct: 361 VAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAVRGGRKLP 420

Query: 421 LELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 458
           LELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL
Sbjct: 421 LELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 456

BLAST of Lsi10G001210 vs. ExPASy TrEMBL
Match: A0A6J1CFR2 (probable membrane metalloprotease ARASP2, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111011017 PE=3 SV=1)

HSP 1 Score: 788.5 bits (2035), Expect = 1.5e-224
Identity = 423/457 (92.56%), Postives = 442/457 (96.72%), Query Frame = 0

Query: 1   MLVNLSSSSFSVIKLANLKSPFSDFSVKSRTHFSKSPSSSSSSSSSSSSLCHSFPSSDLS 60
           ML+NLSS+SFSVIKLAN KSP  DFSVKSRTHFS+S  SSSSSSSSSSSLCHSF SSDLS
Sbjct: 1   MLLNLSSTSFSVIKLANSKSPIPDFSVKSRTHFSES-LSSSSSSSSSSSLCHSFSSSDLS 60

Query: 61  FKNRHQIFWDKAKIPCRRNGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAA 120
           FK RH+IFW+K++IPCR++ DFRLWAFAG+DIGSAQSVLEAAAVLTAIIVVHESGHFLAA
Sbjct: 61  FKTRHKIFWEKSRIPCRKSADFRLWAFAGLDIGSAQSVLEAAAVLTAIIVVHESGHFLAA 120

Query: 121 CLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPESDIPVDDENLLKN 180
           CLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPESDIPVDDENLLKN
Sbjct: 121 CLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPESDIPVDDENLLKN 180

Query: 181 RPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLL 240
           RPILDRV+VISAGVIANIIFAYIIIL+QVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLL
Sbjct: 181 RPILDRVIVISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLL 240

Query: 241 PGDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGKQDLEIGVTPDESFDGT 300
           PGDVILAVNGNELPKLGSTAVS+LV+AIK SP RTVLLKVERGKQD+EIGV PDESFDGT
Sbjct: 241 PGDVILAVNGNELPKLGSTAVSELVDAIKNSPKRTVLLKVERGKQDIEIGVIPDESFDGT 300

Query: 301 GRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFLNFSQSASKVSGP 360
           GRIGVQLSPNVKISKVVA+N LEAFNYSR EFLGLSYNVLDSLKQTFLNFSQSASKVSGP
Sbjct: 301 GRIGVQLSPNVKISKVVARNLLEAFNYSRTEFLGLSYNVLDSLKQTFLNFSQSASKVSGP 360

Query: 361 VAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAVRGGRKLP 420
           VAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEA RGGRKLP
Sbjct: 361 VAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLP 420

Query: 421 LELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 458
           LELEQRIMSSG+M VVLLGLFLI+RDTLNL+FIKDLL
Sbjct: 421 LELEQRIMSSGIMLVVLLGLFLIVRDTLNLDFIKDLL 456

BLAST of Lsi10G001210 vs. ExPASy TrEMBL
Match: A0A6J1JFQ4 (probable membrane metalloprotease ARASP2, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111484092 PE=3 SV=1)

HSP 1 Score: 788.1 bits (2034), Expect = 2.0e-224
Identity = 422/457 (92.34%), Postives = 437/457 (95.62%), Query Frame = 0

Query: 1   MLVNLSSSSFSVIKLANLKSPFSDFSVKSRTHFSKSPSSSSSSSSSSSSLCHSFPSSDLS 60
           ML+NLSSSS+SVIK AN KSPFSDFSVK RTHFSK        S  SSSLCHS  SS+LS
Sbjct: 1   MLLNLSSSSYSVIKFANSKSPFSDFSVKYRTHFSK--------SQFSSSLCHSLTSSELS 60

Query: 61  FKNRHQIFWDKAKIPCRRNGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAA 120
           FKNRHQIFW+K+KIPCRR+GDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAA
Sbjct: 61  FKNRHQIFWEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAA 120

Query: 121 CLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPESDIPVDDENLLKN 180
           CLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDP+SDIP+DDENLLKN
Sbjct: 121 CLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKN 180

Query: 181 RPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLL 240
           RPILDRVLVISAGVIANIIFAYIIIL+QVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLL
Sbjct: 181 RPILDRVLVISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLL 240

Query: 241 PGDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGKQDLEIGVTPDESFDGT 300
           PGDVILAVNGNELPKLG TAVS+LVE IKRSPNRTVLLKVERG+QDLEIGVTPDESFDGT
Sbjct: 241 PGDVILAVNGNELPKLGPTAVSELVETIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGT 300

Query: 301 GRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFLNFSQSASKVSGP 360
           GRIGVQLSPNVKISK+VAKNFLEAF+YSRKEFLGLSYNVLDSLKQT LNFSQSASKVSGP
Sbjct: 301 GRIGVQLSPNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNFSQSASKVSGP 360

Query: 361 VAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAVRGGRKLP 420
           VAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEA RGGRKLP
Sbjct: 361 VAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLP 420

Query: 421 LELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 458
           LELEQRIMSSG+M VVLLGLFLI+RDTLNLEFIKDLL
Sbjct: 421 LELEQRIMSSGIMVVVLLGLFLIVRDTLNLEFIKDLL 449

BLAST of Lsi10G001210 vs. NCBI nr
Match: XP_038902635.1 (probable membrane metalloprotease ARASP2, chloroplastic [Benincasa hispida])

HSP 1 Score: 827.0 bits (2135), Expect = 7.9e-236
Identity = 448/457 (98.03%), Postives = 450/457 (98.47%), Query Frame = 0

Query: 1   MLVNLSSSSFSVIKLANLKSPFSDFSVKSRTHFSKSPSSSSSSSSSSSSLCHSFPSSDLS 60
           MLVNLSSSSFSVIKLA LKSPFSDFSVKSRTHFSK  S SSSSSSSSSS CHSFP SDLS
Sbjct: 1   MLVNLSSSSFSVIKLAKLKSPFSDFSVKSRTHFSK--SLSSSSSSSSSSFCHSFPLSDLS 60

Query: 61  FKNRHQIFWDKAKIPCRRNGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAA 120
           FKNRHQIFWDK KIPCRRNGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAA
Sbjct: 61  FKNRHQIFWDKTKIPCRRNGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAA 120

Query: 121 CLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPESDIPVDDENLLKN 180
           CLQGIHVSKFAIGFGP+LAKFNKNNVEYSIRAFPLGGFVGFPDNDPESDIPVDDENLLKN
Sbjct: 121 CLQGIHVSKFAIGFGPVLAKFNKNNVEYSIRAFPLGGFVGFPDNDPESDIPVDDENLLKN 180

Query: 181 RPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLL 240
           RPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLL
Sbjct: 181 RPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLL 240

Query: 241 PGDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGKQDLEIGVTPDESFDGT 300
           PGDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGKQDLEIGVTPDESFDGT
Sbjct: 241 PGDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGKQDLEIGVTPDESFDGT 300

Query: 301 GRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFLNFSQSASKVSGP 360
           GRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSY+VLDSLKQTFLNFSQSASKVSGP
Sbjct: 301 GRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYSVLDSLKQTFLNFSQSASKVSGP 360

Query: 361 VAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAVRGGRKLP 420
           VAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAVRGGRKLP
Sbjct: 361 VAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAVRGGRKLP 420

Query: 421 LELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 458
           LELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL
Sbjct: 421 LELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 455

BLAST of Lsi10G001210 vs. NCBI nr
Match: XP_008455887.1 (PREDICTED: probable membrane metalloprotease ARASP2, chloroplastic [Cucumis melo] >TYK28920.1 putative membrane metalloprotease ARASP2 [Cucumis melo var. makuwa])

HSP 1 Score: 817.0 bits (2109), Expect = 8.2e-233
Identity = 444/457 (97.16%), Postives = 448/457 (98.03%), Query Frame = 0

Query: 1   MLVNLSSSSFSVIKLANLKSPFSDFSVKSRTHFSKSPSSSSSSSSSSSSLCHSFPSSDLS 60
           ML+NL SSSFSVIKLANLKSPFSDFSVKSRTHFSKS SSSSSSSSSSSSL HS PSS+LS
Sbjct: 1   MLLNLPSSSFSVIKLANLKSPFSDFSVKSRTHFSKSLSSSSSSSSSSSSLWHSPPSSELS 60

Query: 61  FKNRHQIFWDKAKIPCRRNGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAA 120
           FKNRHQIFWDK KI CRRNGDFRLWAFAG+DIGSAQSVLEAAAVLTAIIVVHESGHFLAA
Sbjct: 61  FKNRHQIFWDKTKIRCRRNGDFRLWAFAGMDIGSAQSVLEAAAVLTAIIVVHESGHFLAA 120

Query: 121 CLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPESDIPVDDENLLKN 180
           CLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPESDIPVDDENLLKN
Sbjct: 121 CLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPESDIPVDDENLLKN 180

Query: 181 RPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLL 240
           RPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEV TLSAASRDGLL
Sbjct: 181 RPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEVLTLSAASRDGLL 240

Query: 241 PGDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGKQDLEIGVTPDESFDGT 300
           PGDVILAVNGNELP LGSTAVSDLVEAIKRSPNRTVLLKVERG QDLEIGVTPD+SFDGT
Sbjct: 241 PGDVILAVNGNELPNLGSTAVSDLVEAIKRSPNRTVLLKVERGNQDLEIGVTPDKSFDGT 300

Query: 301 GRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFLNFSQSASKVSGP 360
           GRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFLNFSQSASKVSGP
Sbjct: 301 GRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFLNFSQSASKVSGP 360

Query: 361 VAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAVRGGRKLP 420
           VAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAVRGGRKLP
Sbjct: 361 VAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAVRGGRKLP 420

Query: 421 LELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 458
           LELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL
Sbjct: 421 LELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 457

BLAST of Lsi10G001210 vs. NCBI nr
Match: XP_004151933.2 (probable membrane metalloprotease ARASP2, chloroplastic [Cucumis sativus])

HSP 1 Score: 811.6 bits (2095), Expect = 3.4e-231
Identity = 443/457 (96.94%), Postives = 448/457 (98.03%), Query Frame = 0

Query: 1   MLVNLSSSSFSVIKLANLKSPFSDFSVKSRTHFSKSPSSSSSSSSSSSSLCHSFPSSDLS 60
           ML+NL SSSFSVIKLANLKSPFSDFSVKSRTHFSKS  SSSSSSSSSSSL HS PSS+LS
Sbjct: 1   MLLNLPSSSFSVIKLANLKSPFSDFSVKSRTHFSKS-LSSSSSSSSSSSLWHSPPSSELS 60

Query: 61  FKNRHQIFWDKAKIPCRRNGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAA 120
           FK+RHQIFWDK KI CRRNGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAA
Sbjct: 61  FKDRHQIFWDKTKIRCRRNGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAA 120

Query: 121 CLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPESDIPVDDENLLKN 180
           CLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDP+SDIPVDDENLLKN
Sbjct: 121 CLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPVDDENLLKN 180

Query: 181 RPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLL 240
           RPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEV TLSAASRDGLL
Sbjct: 181 RPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEVLTLSAASRDGLL 240

Query: 241 PGDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGKQDLEIGVTPDESFDGT 300
           PGDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERG QDLEIGVTPD+SFDGT
Sbjct: 241 PGDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGNQDLEIGVTPDKSFDGT 300

Query: 301 GRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFLNFSQSASKVSGP 360
           GRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFLNFSQSASKVSGP
Sbjct: 301 GRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFLNFSQSASKVSGP 360

Query: 361 VAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAVRGGRKLP 420
           VAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAVRGGRKLP
Sbjct: 361 VAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAVRGGRKLP 420

Query: 421 LELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 458
           LELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL
Sbjct: 421 LELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 456

BLAST of Lsi10G001210 vs. NCBI nr
Match: XP_022140316.1 (probable membrane metalloprotease ARASP2, chloroplastic [Momordica charantia])

HSP 1 Score: 788.5 bits (2035), Expect = 3.1e-224
Identity = 423/457 (92.56%), Postives = 442/457 (96.72%), Query Frame = 0

Query: 1   MLVNLSSSSFSVIKLANLKSPFSDFSVKSRTHFSKSPSSSSSSSSSSSSLCHSFPSSDLS 60
           ML+NLSS+SFSVIKLAN KSP  DFSVKSRTHFS+S  SSSSSSSSSSSLCHSF SSDLS
Sbjct: 1   MLLNLSSTSFSVIKLANSKSPIPDFSVKSRTHFSES-LSSSSSSSSSSSLCHSFSSSDLS 60

Query: 61  FKNRHQIFWDKAKIPCRRNGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAA 120
           FK RH+IFW+K++IPCR++ DFRLWAFAG+DIGSAQSVLEAAAVLTAIIVVHESGHFLAA
Sbjct: 61  FKTRHKIFWEKSRIPCRKSADFRLWAFAGLDIGSAQSVLEAAAVLTAIIVVHESGHFLAA 120

Query: 121 CLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPESDIPVDDENLLKN 180
           CLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPESDIPVDDENLLKN
Sbjct: 121 CLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPESDIPVDDENLLKN 180

Query: 181 RPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLL 240
           RPILDRV+VISAGVIANIIFAYIIIL+QVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLL
Sbjct: 181 RPILDRVIVISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLL 240

Query: 241 PGDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGKQDLEIGVTPDESFDGT 300
           PGDVILAVNGNELPKLGSTAVS+LV+AIK SP RTVLLKVERGKQD+EIGV PDESFDGT
Sbjct: 241 PGDVILAVNGNELPKLGSTAVSELVDAIKNSPKRTVLLKVERGKQDIEIGVIPDESFDGT 300

Query: 301 GRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFLNFSQSASKVSGP 360
           GRIGVQLSPNVKISKVVA+N LEAFNYSR EFLGLSYNVLDSLKQTFLNFSQSASKVSGP
Sbjct: 301 GRIGVQLSPNVKISKVVARNLLEAFNYSRTEFLGLSYNVLDSLKQTFLNFSQSASKVSGP 360

Query: 361 VAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAVRGGRKLP 420
           VAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEA RGGRKLP
Sbjct: 361 VAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLP 420

Query: 421 LELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 458
           LELEQRIMSSG+M VVLLGLFLI+RDTLNL+FIKDLL
Sbjct: 421 LELEQRIMSSGIMLVVLLGLFLIVRDTLNLDFIKDLL 456

BLAST of Lsi10G001210 vs. NCBI nr
Match: XP_022986309.1 (probable membrane metalloprotease ARASP2, chloroplastic [Cucurbita maxima])

HSP 1 Score: 788.1 bits (2034), Expect = 4.1e-224
Identity = 422/457 (92.34%), Postives = 437/457 (95.62%), Query Frame = 0

Query: 1   MLVNLSSSSFSVIKLANLKSPFSDFSVKSRTHFSKSPSSSSSSSSSSSSLCHSFPSSDLS 60
           ML+NLSSSS+SVIK AN KSPFSDFSVK RTHFSK        S  SSSLCHS  SS+LS
Sbjct: 1   MLLNLSSSSYSVIKFANSKSPFSDFSVKYRTHFSK--------SQFSSSLCHSLTSSELS 60

Query: 61  FKNRHQIFWDKAKIPCRRNGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAA 120
           FKNRHQIFW+K+KIPCRR+GDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAA
Sbjct: 61  FKNRHQIFWEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAA 120

Query: 121 CLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPESDIPVDDENLLKN 180
           CLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDP+SDIP+DDENLLKN
Sbjct: 121 CLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKN 180

Query: 181 RPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLL 240
           RPILDRVLVISAGVIANIIFAYIIIL+QVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLL
Sbjct: 181 RPILDRVLVISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLL 240

Query: 241 PGDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGKQDLEIGVTPDESFDGT 300
           PGDVILAVNGNELPKLG TAVS+LVE IKRSPNRTVLLKVERG+QDLEIGVTPDESFDGT
Sbjct: 241 PGDVILAVNGNELPKLGPTAVSELVETIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGT 300

Query: 301 GRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFLNFSQSASKVSGP 360
           GRIGVQLSPNVKISK+VAKNFLEAF+YSRKEFLGLSYNVLDSLKQT LNFSQSASKVSGP
Sbjct: 301 GRIGVQLSPNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNFSQSASKVSGP 360

Query: 361 VAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAVRGGRKLP 420
           VAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEA RGGRKLP
Sbjct: 361 VAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLP 420

Query: 421 LELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 458
           LELEQRIMSSG+M VVLLGLFLI+RDTLNLEFIKDLL
Sbjct: 421 LELEQRIMSSGIMVVVLLGLFLIVRDTLNLEFIKDLL 449

BLAST of Lsi10G001210 vs. TAIR 10
Match: AT2G32480.1 (ARABIDOPSIS SERIN PROTEASE )

HSP 1 Score: 569.7 bits (1467), Expect = 2.1e-162
Identity = 312/464 (67.24%), Postives = 370/464 (79.74%), Query Frame = 0

Query: 1   MLVNLSSSSFSVIK---LANLKSPFSDFSVKSRTHFSKSPSSSSSSSSSSSSLCHSFPS- 60
           ML+N+SSS  S      L+N  +P S F  +S+TH SKS               H FP  
Sbjct: 1   MLLNISSSPISHRNPHFLSNFNNPISYFPRRSKTHLSKS---------------HFFPKF 60

Query: 61  ---SDLSFKNRHQIFWDKAKIPCRRNGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHE 120
              S+ S KNR  + +   + P     DFR  A +GID+GS +SVLEA AVLT IIVVHE
Sbjct: 61  TPLSNQSLKNR--VLFGNKRYPDGERFDFRSRAISGIDLGSFESVLEAIAVLTTIIVVHE 120

Query: 121 SGHFLAACLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPESDIPVD 180
           SGHFLAA LQGIHVSKFAIGFGPILAKF+ NNVEYS+RAFPLGGFVGFPDNDP+S+IP+D
Sbjct: 121 SGHFLAASLQGIHVSKFAIGFGPILAKFDYNNVEYSLRAFPLGGFVGFPDNDPDSEIPID 180

Query: 181 DENLLKNRPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEVRTLSA 240
           DENLLKNRP LDR +V+SAG+IAN+IFAY II VQV SVGLPVQE FPGVLVPEV+T SA
Sbjct: 181 DENLLKNRPTLDRSIVVSAGIIANVIFAYAIIFVQVLSVGLPVQEAFPGVLVPEVKTFSA 240

Query: 241 ASRDGLLPGDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGKQDLEIGVTP 300
           ASRDGLL GDVILAV+G EL K G  AVS +V+ +KR+P   V+ ++ERG +D +I VTP
Sbjct: 241 ASRDGLLSGDVILAVDGTELSKTGPDAVSKIVDIVKRNPKSNVVFRIERGGEDFDIRVTP 300

Query: 301 DESFDGTGRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFLNFSQS 360
           D++FDGTG+IGVQLSPNV+I+KV  +N  E F +  +EF+GLS NVLD LKQTF NFSQ+
Sbjct: 301 DKNFDGTGKIGVQLSPNVRITKVRPRNIPETFRFVGREFMGLSSNVLDGLKQTFFNFSQT 360

Query: 361 ASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAV 420
           ASKV+GPVAIIAVGAEVARSNIDGLYQFAA+LNINLAVINLLPLPALDGG+LALIL+EAV
Sbjct: 361 ASKVAGPVAIIAVGAEVARSNIDGLYQFAALLNINLAVINLLPLPALDGGTLALILLEAV 420

Query: 421 RGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 458
           RGG+KLP+E+EQ IMSSG+M V+ LGLFLI++DTL+L+FIK++L
Sbjct: 421 RGGKKLPVEVEQGIMSSGIMLVIFLGLFLIVKDTLSLDFIKEML 447

BLAST of Lsi10G001210 vs. TAIR 10
Match: AT1G05140.1 (Peptidase M50 family protein )

HSP 1 Score: 562.8 bits (1449), Expect = 2.6e-160
Identity = 312/460 (67.83%), Postives = 369/460 (80.22%), Query Frame = 0

Query: 1   MLVNLSSSSFS---VIKLANLKSPFSDFSVKSRTHFSKSPSSSSSSSSSSSSLCHSFPSS 60
           ML+N+SSS  S      L++  +P S+F  KS+TH  KS  S+ S+ S   +   +F   
Sbjct: 1   MLLNISSSPISHRIPHFLSDFNNPTSNFPPKSKTHLPKSNLSTLSNHSLYGTKNRAF--- 60

Query: 61  DLSFKNRHQIFWDKAKIPCRRNGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHF 120
              +KN             +RN   R  A    D GS +SVLEA+AVLTAIIVVHE+GHF
Sbjct: 61  ---YKN-------------KRNPYNRTQALGRFDFGSLESVLEASAVLTAIIVVHETGHF 120

Query: 121 LAACLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPESDIPVDDENL 180
           LAA LQGI VSKFAIGFGPILAKFN NNVEYS+RAFPLGGFVGFPDNDP+SDIPVDD NL
Sbjct: 121 LAASLQGIRVSKFAIGFGPILAKFNSNNVEYSLRAFPLGGFVGFPDNDPDSDIPVDDRNL 180

Query: 181 LKNRPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEVRTLSAASRD 240
           LKNRPILDRV+V+SAG++AN+IFAY II  QV SVGLPVQE FPGVLVP+V++ SAASRD
Sbjct: 181 LKNRPILDRVIVVSAGIVANVIFAYAIIFTQVVSVGLPVQESFPGVLVPDVKSFSAASRD 240

Query: 241 GLLPGDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGKQDLEIGVTPDESF 300
           GLLPGDVILAV+G EL   GS +VS +V+ +KR+P   VLL++ERGK+  EI +TPD+SF
Sbjct: 241 GLLPGDVILAVDGTELSNSGSDSVSKVVDVVKRNPEHNVLLRIERGKESFEIRITPDKSF 300

Query: 301 DGTGRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFLNFSQSASKV 360
           DGTG+IGVQLSPNV+  KV  KN  E F+++ +EF GLSYNVLDSLKQTFLNFSQ+ASKV
Sbjct: 301 DGTGKIGVQLSPNVRFGKVRPKNIPETFSFAGREFFGLSYNVLDSLKQTFLNFSQTASKV 360

Query: 361 SGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAVRGGR 420
           +GPVAIIAVGAEVARSN DGLYQFAA+LN+NLAVINLLPLPALDGG+LALIL+EAVRGGR
Sbjct: 361 AGPVAIIAVGAEVARSNADGLYQFAALLNLNLAVINLLPLPALDGGTLALILLEAVRGGR 420

Query: 421 KLPLELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 458
           KLPLE+EQ IMSSG+M V+ LGLFLI++DTLNL+FIK++L
Sbjct: 421 KLPLEVEQGIMSSGIMLVLFLGLFLIVKDTLNLDFIKEML 441

BLAST of Lsi10G001210 vs. TAIR 10
Match: AT2G32480.2 (ARABIDOPSIS SERIN PROTEASE )

HSP 1 Score: 509.6 bits (1311), Expect = 2.6e-144
Identity = 290/464 (62.50%), Postives = 344/464 (74.14%), Query Frame = 0

Query: 1   MLVNLSSSSFSVIK---LANLKSPFSDFSVKSRTHFSKSPSSSSSSSSSSSSLCHSFPS- 60
           ML+N+SSS  S      L+N  +P S F  +S+TH SKS               H FP  
Sbjct: 1   MLLNISSSPISHRNPHFLSNFNNPISYFPRRSKTHLSKS---------------HFFPKF 60

Query: 61  ---SDLSFKNRHQIFWDKAKIPCRRNGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHE 120
              S+ S KNR  + +   + P     DFR  A +GID+GS +SVLEA AVLT IIVVHE
Sbjct: 61  TPLSNQSLKNR--VLFGNKRYPDGERFDFRSRAISGIDLGSFESVLEAIAVLTTIIVVHE 120

Query: 121 SGHFLAACLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPESDIPVD 180
           SGHFLAA LQGIHVSKFAIGFGPILAKF+ NNVEYS+RAFPLGGFVGFPDNDP+S+IP+D
Sbjct: 121 SGHFLAASLQGIHVSKFAIGFGPILAKFDYNNVEYSLRAFPLGGFVGFPDNDPDSEIPID 180

Query: 181 DENLLKNRPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEVRTLSA 240
           DENLLKNRP LDR +V+SAG+IAN+IFAY II VQV SVGLPVQE FPGVLVPEV+T SA
Sbjct: 181 DENLLKNRPTLDRSIVVSAGIIANVIFAYAIIFVQVLSVGLPVQEAFPGVLVPEVKTFSA 240

Query: 241 ASRDGLLPGDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGKQDLEIGVTP 300
           ASRDGLL GDVILAV+G EL K G  AVS +V+ +KR+P   V+ ++ERG +D +I VTP
Sbjct: 241 ASRDGLLSGDVILAVDGTELSKTGPDAVSKIVDIVKRNPKSNVVFRIERGGEDFDIRVTP 300

Query: 301 DESFDGTGRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFLNFSQS 360
           D++FDGTG+IGVQLSPNV+I+K                                     +
Sbjct: 301 DKNFDGTGKIGVQLSPNVRITK-------------------------------------T 360

Query: 361 ASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAV 420
           ASKV+GPVAIIAVGAEVARSNIDGLYQFAA+LNINLAVINLLPLPALDGG+LALIL+EAV
Sbjct: 361 ASKVAGPVAIIAVGAEVARSNIDGLYQFAALLNINLAVINLLPLPALDGGTLALILLEAV 410

Query: 421 RGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 458
           RGG+KLP+E+EQ IMSSG+M V+ LGLFLI++DTL+L+FIK++L
Sbjct: 421 RGGKKLPVEVEQGIMSSGIMLVIFLGLFLIVKDTLSLDFIKEML 410

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O808853.0e-16167.24Membrane metalloprotease ARASP, chloroplastic OS=Arabidopsis thaliana OX=3702 GN... [more]
O230533.6e-15967.83Probable membrane metalloprotease ARASP2, chloroplastic OS=Arabidopsis thaliana ... [more]
P737141.8e-8948.35Putative zinc metalloprotease slr1821 OS=Synechocystis sp. (strain PCC 6803 / Ka... [more]
Q8YQ642.0e-8048.04Putative zinc metalloprotease all3971 OS=Nostoc sp. (strain PCC 7120 / SAG 25.82... [more]
O317542.4e-3329.20Regulator of sigma-W protease RasP OS=Bacillus subtilis (strain 168) OX=224308 G... [more]
Match NameE-valueIdentityDescription
A0A5D3E0824.0e-23397.16Putative membrane metalloprotease ARASP2 OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A1S3C3764.0e-23397.16probable membrane metalloprotease ARASP2, chloroplastic OS=Cucumis melo OX=3656 ... [more]
A0A0A0LT841.7e-23196.94PDZ domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G417840 PE=3 SV... [more]
A0A6J1CFR21.5e-22492.56probable membrane metalloprotease ARASP2, chloroplastic OS=Momordica charantia O... [more]
A0A6J1JFQ42.0e-22492.34probable membrane metalloprotease ARASP2, chloroplastic OS=Cucurbita maxima OX=3... [more]
Match NameE-valueIdentityDescription
XP_038902635.17.9e-23698.03probable membrane metalloprotease ARASP2, chloroplastic [Benincasa hispida][more]
XP_008455887.18.2e-23397.16PREDICTED: probable membrane metalloprotease ARASP2, chloroplastic [Cucumis melo... [more]
XP_004151933.23.4e-23196.94probable membrane metalloprotease ARASP2, chloroplastic [Cucumis sativus][more]
XP_022140316.13.1e-22492.56probable membrane metalloprotease ARASP2, chloroplastic [Momordica charantia][more]
XP_022986309.14.1e-22492.34probable membrane metalloprotease ARASP2, chloroplastic [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT2G32480.12.1e-16267.24ARABIDOPSIS SERIN PROTEASE [more]
AT1G05140.12.6e-16067.83Peptidase M50 family protein [more]
AT2G32480.22.6e-14462.50ARABIDOPSIS SERIN PROTEASE [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001478PDZ domainSMARTSM00228pdz_newcoord: 210..284
e-value: 1.0E-10
score: 51.6
IPR001478PDZ domainPROSITEPS50106PDZcoord: 212..283
score: 11.2599
IPR041489PDZ domain 6PFAMPF17820PDZ_6coord: 225..282
e-value: 8.4E-9
score: 35.2
IPR008915Peptidase M50PFAMPF02163Peptidase_M50coord: 102..438
e-value: 1.1E-60
score: 205.1
IPR036034PDZ superfamilyGENE3D2.30.42.10coord: 212..308
e-value: 4.9E-16
score: 60.8
IPR036034PDZ superfamilySUPERFAMILY50156PDZ domain-likecoord: 210..295
IPR004387Peptidase M50, putative membrane-associated zinc metallopeptidasePANTHERPTHR42837REGULATOR OF SIGMA-E PROTEASE RSEPcoord: 23..457
NoneNo IPR availablePANTHERPTHR42837:SF4MEMBRANE METALLOPROTEASE ARASP2, CHLOROPLASTIC-RELATEDcoord: 23..457
NoneNo IPR availableCDDcd06163S2P-M50_PDZ_RseP-likecoord: 98..449
e-value: 2.01458E-42
score: 146.405
NoneNo IPR availableCDDcd00989PDZ_metalloproteasecoord: 210..293
e-value: 6.03639E-15
score: 67.6439

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi10G001210.1Lsi10G001210.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004222 metalloendopeptidase activity
molecular_function GO:0005515 protein binding