Lsi09G016780 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi09G016780
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionDUF761 domain-containing protein
Locationchr09: 25214204 .. 25215985 (+)
RNA-Seq ExpressionLsi09G016780
SyntenyLsi09G016780
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGTCTTCAACTTCCAGCCCTTTCACCAAACCCCATTTTCCCCATTCTCCACTTCCATCAATATCTACCACTCACCATAGCAAGTCCTGCACACAGTTCCTCTGTAAATCCCTCTTCTTCTGCATTTTTCTCCTCCTCCTTCCTCTCTTCCCTTCCGAAGCTCCAGAATTCGTCAATCAGACTTTGCTCACCAAATTCTGGGAGCTCTTTCATCTCTTGTTCGTCGGCATTGCTGTTTCCTATGGTCTCTTCAGCAGAAGGAACATCCAGGTGAGTGTAGAAGAACCTCGCTTCTCCAATTTTGAAAATCCGCAGTCGTATTTGTCTAAGATGTTTCAAGTCGCTTCGATTTTTGAAGATGTTGACGATTTGAGTGTTTCTGATGAGAGGAAATTGAGTGAAGTTTTGTACATTCAGCCGAATCTTGGATCTGTGAGTGATTTTAATGCCCAATCTCACGAACTGGAAAAATACCGTTATTCAATACCCAAAAAAAGGTATGAAAATTCTCATGAATTTGCTGATACTGATAATGTCGGTCATGTTTGTAAATCGAGATATACTCGGGGTGGATCTGTGGTGGTTGTTACTGAAACAAATCGTAGTTCTGGTGAATGGTTGGAATCAGGAGCCATTGTAAATTATAAACCTCTAGGTTTGCCTGTTAGGAGTCTGAGGTCGAGTCTTACTGAGCCCGACGATGTTGAATTTGATTGTAATGATGAATCTTGTTTGAGTTCTAAAAGTTCATCCAAGAGCTCTGAGAATAATTGTGAAAGAAGTGAATTTGGTGATAATTGTTGTGTGAATTTGGAGGAGAAGTTTGATGAGACTGTAATTGCATCAATGTCCCCATTTCAATTGCGTGAGAAATTTGGAAAGAAGATGATGAGAGAGAGAGGAGTTAGGAATGCTGTTCTTCGCCCTTCCCATTTTAGACCTCCTTCCATTGATGAAACTCAATTTGAATCACTAAAAAAATCAAGTTCTTTTCATTCTAATCTTTCTCAGTCATCACAAACTAGTTCCCTCTCTTCTTCGTTGTCATCGACAACGAGAAAGCACCGTAAAATGTCGTCGCTCGGTAACATTTCCTATAAATCGTTGCATTCTCGACAATATAGTATGAGTTCTCTGTCTGAAAACAGTAGAGGGAGCTCTGAAGACCCTCTGATTGAACCAGAAAATTCATCTGAATGCAACGAATCCGTGGTAAGTTCCCCGCGTTTGGACAGGAATTTCGCAAGTATTCCGAAAGCTTTATCTCGGGGAAAATCCGTTAGAACAATTAGAGCAAATGCAATTGCCATAGAGGAAATGAAAGCTCAAGAGGAGATGTATAGAAACCAAGTTGAACATGATGACAATATAGGGAATAAGTTTGAAGGTGGAATGTCACCATATATGAGAGAAGATGGAATAGGACATGGATGGTCTGGTGTTGTTAACCCGAATGCTGGTAACTCTAATCGTTTTTCGAAGATGGCATTCTCGGGGATTGAGGAGCAAAAGGAAGACACTGAGAGTCAGCTAACAGATGATGGTAAAGATAACTCTGAGAGGGAGGATGAAAGTTTGTTTGCGAGTTCAGATGAAGAAGCTGCTTTGAGTATGGCGGGCGATTCGGAATCGGGGGCTCACGAGGTTGACAAGAAGGCAGGCGAGTTCATAGCCAAGTTCAGGGAGCAAATACAGCTTCAGAGGATGGCTTCAGTAGATAAAAGATTGAGAGGAGGATGGGGCTCATTCAGCAGCACAAGCAGCAGCTATTTTAGTTGA

mRNA sequence

ATGGCGTCTTCAACTTCCAGCCCTTTCACCAAACCCCATTTTCCCCATTCTCCACTTCCATCAATATCTACCACTCACCATAGCAAGTCCTGCACACAGTTCCTCTGTAAATCCCTCTTCTTCTGCATTTTTCTCCTCCTCCTTCCTCTCTTCCCTTCCGAAGCTCCAGAATTCGTCAATCAGACTTTGCTCACCAAATTCTGGGAGCTCTTTCATCTCTTGTTCGTCGGCATTGCTGTTTCCTATGGTCTCTTCAGCAGAAGGAACATCCAGGTGAGTGTAGAAGAACCTCGCTTCTCCAATTTTGAAAATCCGCAGTCGTATTTGTCTAAGATGTTTCAAGTCGCTTCGATTTTTGAAGATGTTGACGATTTGAGTGTTTCTGATGAGAGGAAATTGAGTGAAGTTTTGTACATTCAGCCGAATCTTGGATCTGTGAGTGATTTTAATGCCCAATCTCACGAACTGGAAAAATACCGTTATTCAATACCCAAAAAAAGGTATGAAAATTCTCATGAATTTGCTGATACTGATAATGTCGGTCATGTTTGTAAATCGAGATATACTCGGGGTGGATCTGTGGTGGTTGTTACTGAAACAAATCGTAGTTCTGGTGAATGGTTGGAATCAGGAGCCATTGTAAATTATAAACCTCTAGGTTTGCCTGTTAGGAGTCTGAGGTCGAGTCTTACTGAGCCCGACGATGTTGAATTTGATTGTAATGATGAATCTTGTTTGAGTTCTAAAAGTTCATCCAAGAGCTCTGAGAATAATTGTGAAAGAAGTGAATTTGGTGATAATTGTTGTGTGAATTTGGAGGAGAAGTTTGATGAGACTGTAATTGCATCAATGTCCCCATTTCAATTGCGTGAGAAATTTGGAAAGAAGATGATGAGAGAGAGAGGAGTTAGGAATGCTGTTCTTCGCCCTTCCCATTTTAGACCTCCTTCCATTGATGAAACTCAATTTGAATCACTAAAAAAATCAAGTTCTTTTCATTCTAATCTTTCTCAGTCATCACAAACTAGTTCCCTCTCTTCTTCGTTGTCATCGACAACGAGAAAGCACCGTAAAATGTCGTCGCTCGGTAACATTTCCTATAAATCGTTGCATTCTCGACAATATAGTATGAGTTCTCTGTCTGAAAACAGTAGAGGGAGCTCTGAAGACCCTCTGATTGAACCAGAAAATTCATCTGAATGCAACGAATCCGTGGTAAGTTCCCCGCGTTTGGACAGGAATTTCGCAAGTATTCCGAAAGCTTTATCTCGGGGAAAATCCGTTAGAACAATTAGAGCAAATGCAATTGCCATAGAGGAAATGAAAGCTCAAGAGGAGATGTATAGAAACCAAGTTGAACATGATGACAATATAGGGAATAAGTTTGAAGGTGGAATGTCACCATATATGAGAGAAGATGGAATAGGACATGGATGGTCTGGTGTTGTTAACCCGAATGCTGGTAACTCTAATCGTTTTTCGAAGATGGCATTCTCGGGGATTGAGGAGCAAAAGGAAGACACTGAGAGTCAGCTAACAGATGATGGTAAAGATAACTCTGAGAGGGAGGATGAAAGTTTGTTTGCGAGTTCAGATGAAGAAGCTGCTTTGAGTATGGCGGGCGATTCGGAATCGGGGGCTCACGAGGTTGACAAGAAGGCAGGCGAGTTCATAGCCAAGTTCAGGGAGCAAATACAGCTTCAGAGGATGGCTTCAGTAGATAAAAGATTGAGAGGAGGATGGGGCTCATTCAGCAGCACAAGCAGCAGCTATTTTAGTTGA

Coding sequence (CDS)

ATGGCGTCTTCAACTTCCAGCCCTTTCACCAAACCCCATTTTCCCCATTCTCCACTTCCATCAATATCTACCACTCACCATAGCAAGTCCTGCACACAGTTCCTCTGTAAATCCCTCTTCTTCTGCATTTTTCTCCTCCTCCTTCCTCTCTTCCCTTCCGAAGCTCCAGAATTCGTCAATCAGACTTTGCTCACCAAATTCTGGGAGCTCTTTCATCTCTTGTTCGTCGGCATTGCTGTTTCCTATGGTCTCTTCAGCAGAAGGAACATCCAGGTGAGTGTAGAAGAACCTCGCTTCTCCAATTTTGAAAATCCGCAGTCGTATTTGTCTAAGATGTTTCAAGTCGCTTCGATTTTTGAAGATGTTGACGATTTGAGTGTTTCTGATGAGAGGAAATTGAGTGAAGTTTTGTACATTCAGCCGAATCTTGGATCTGTGAGTGATTTTAATGCCCAATCTCACGAACTGGAAAAATACCGTTATTCAATACCCAAAAAAAGGTATGAAAATTCTCATGAATTTGCTGATACTGATAATGTCGGTCATGTTTGTAAATCGAGATATACTCGGGGTGGATCTGTGGTGGTTGTTACTGAAACAAATCGTAGTTCTGGTGAATGGTTGGAATCAGGAGCCATTGTAAATTATAAACCTCTAGGTTTGCCTGTTAGGAGTCTGAGGTCGAGTCTTACTGAGCCCGACGATGTTGAATTTGATTGTAATGATGAATCTTGTTTGAGTTCTAAAAGTTCATCCAAGAGCTCTGAGAATAATTGTGAAAGAAGTGAATTTGGTGATAATTGTTGTGTGAATTTGGAGGAGAAGTTTGATGAGACTGTAATTGCATCAATGTCCCCATTTCAATTGCGTGAGAAATTTGGAAAGAAGATGATGAGAGAGAGAGGAGTTAGGAATGCTGTTCTTCGCCCTTCCCATTTTAGACCTCCTTCCATTGATGAAACTCAATTTGAATCACTAAAAAAATCAAGTTCTTTTCATTCTAATCTTTCTCAGTCATCACAAACTAGTTCCCTCTCTTCTTCGTTGTCATCGACAACGAGAAAGCACCGTAAAATGTCGTCGCTCGGTAACATTTCCTATAAATCGTTGCATTCTCGACAATATAGTATGAGTTCTCTGTCTGAAAACAGTAGAGGGAGCTCTGAAGACCCTCTGATTGAACCAGAAAATTCATCTGAATGCAACGAATCCGTGGTAAGTTCCCCGCGTTTGGACAGGAATTTCGCAAGTATTCCGAAAGCTTTATCTCGGGGAAAATCCGTTAGAACAATTAGAGCAAATGCAATTGCCATAGAGGAAATGAAAGCTCAAGAGGAGATGTATAGAAACCAAGTTGAACATGATGACAATATAGGGAATAAGTTTGAAGGTGGAATGTCACCATATATGAGAGAAGATGGAATAGGACATGGATGGTCTGGTGTTGTTAACCCGAATGCTGGTAACTCTAATCGTTTTTCGAAGATGGCATTCTCGGGGATTGAGGAGCAAAAGGAAGACACTGAGAGTCAGCTAACAGATGATGGTAAAGATAACTCTGAGAGGGAGGATGAAAGTTTGTTTGCGAGTTCAGATGAAGAAGCTGCTTTGAGTATGGCGGGCGATTCGGAATCGGGGGCTCACGAGGTTGACAAGAAGGCAGGCGAGTTCATAGCCAAGTTCAGGGAGCAAATACAGCTTCAGAGGATGGCTTCAGTAGATAAAAGATTGAGAGGAGGATGGGGCTCATTCAGCAGCACAAGCAGCAGCTATTTTAGTTGA

Protein sequence

MASSTSSPFTKPHFPHSPLPSISTTHHSKSCTQFLCKSLFFCIFLLLLPLFPSEAPEFVNQTLLTKFWELFHLLFVGIAVSYGLFSRRNIQVSVEEPRFSNFENPQSYLSKMFQVASIFEDVDDLSVSDERKLSEVLYIQPNLGSVSDFNAQSHELEKYRYSIPKKRYENSHEFADTDNVGHVCKSRYTRGGSVVVVTETNRSSGEWLESGAIVNYKPLGLPVRSLRSSLTEPDDVEFDCNDESCLSSKSSSKSSENNCERSEFGDNCCVNLEEKFDETVIASMSPFQLREKFGKKMMRERGVRNAVLRPSHFRPPSIDETQFESLKKSSSFHSNLSQSSQTSSLSSSLSSTTRKHRKMSSLGNISYKSLHSRQYSMSSLSENSRGSSEDPLIEPENSSECNESVVSSPRLDRNFASIPKALSRGKSVRTIRANAIAIEEMKAQEEMYRNQVEHDDNIGNKFEGGMSPYMREDGIGHGWSGVVNPNAGNSNRFSKMAFSGIEEQKEDTESQLTDDGKDNSEREDESLFASSDEEAALSMAGDSESGAHEVDKKAGEFIAKFREQIQLQRMASVDKRLRGGWGSFSSTSSSYFS
Homology
BLAST of Lsi09G016780 vs. ExPASy TrEMBL
Match: A0A0A0K9X1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G103540 PE=4 SV=1)

HSP 1 Score: 974.5 bits (2518), Expect = 1.9e-280
Identity = 523/601 (87.02%), Postives = 548/601 (91.18%), Query Frame = 0

Query: 1   MASSTSSPFTKPHFPHSPLPSISTTHHSKSCTQFLCKSLFFCIFLLLLPLFPSEAPEFVN 60
           MA S S+PFTKPHFPHSPLP  STT HS SCTQF+CKSLFFCIFLLLLPLFPSEAPEFVN
Sbjct: 1   MAPSPSTPFTKPHFPHSPLPPTSTTRHSNSCTQFICKSLFFCIFLLLLPLFPSEAPEFVN 60

Query: 61  QTLLTKFWELFHLLFVGIAVSYGLFSRRNIQVSV--EEPRFSNFENPQSYLSKMFQVASI 120
           QT LTKFWELFHL+F+GIAVSYGLFSRRN+QVSV  +EPRFSNFENPQSYLSKMF VASI
Sbjct: 61  QTFLTKFWELFHLMFIGIAVSYGLFSRRNVQVSVDSDEPRFSNFENPQSYLSKMFHVASI 120

Query: 121 FEDVDDLSVSDERKLSEVLYIQPNLGSVSDFNAQSHELEKYRYSIPKKRYENSHEFADTD 180
           FEDVDD SVSDERKLSEVLYIQPNLGSVS  NA S + E + YSIPKKRYENS EFA+TD
Sbjct: 121 FEDVDDFSVSDERKLSEVLYIQPNLGSVSGLNAISRQQENFHYSIPKKRYENSLEFAETD 180

Query: 181 NVGHVCKSRYTRGGSVVVVTETNRS-SGEWLESGAIVNYKPLGLPVRSLRSSLTEPDDVE 240
           NVGH CKSRYTRGGSVVVV ETNRS SGEWLESGAIVNYKPLGLPVRSL+SSLTEPDDVE
Sbjct: 181 NVGHACKSRYTRGGSVVVVAETNRSNSGEWLESGAIVNYKPLGLPVRSLKSSLTEPDDVE 240

Query: 241 FDCNDESCLSSKSSSKSSENNCER-SEFGDNCCVNLEEKFDETVIASMSPFQLREKFGKK 300
           FDC DESCLSSKSSSK+SE+NCER SEFGDNCCVNLEEKFDETVIASMSPFQLREKF K 
Sbjct: 241 FDCGDESCLSSKSSSKNSESNCERTSEFGDNCCVNLEEKFDETVIASMSPFQLREKFEKN 300

Query: 301 MMRERGVRNAVLRPSHFRPPSIDETQFESLKKSSSFHSNLSQSSQTSSLSSSLSSTTRKH 360
           MMRER V+NAVLRPSHFRP SIDETQFESLKKS+S HSNLSQSSQTSSLSS LSS TRKH
Sbjct: 301 MMRERRVKNAVLRPSHFRPSSIDETQFESLKKSTSLHSNLSQSSQTSSLSSPLSSRTRKH 360

Query: 361 RKMSSLGNISYKSLHSRQYSMSSLSENSRGSSEDPLIEPENSSECNESVVSSPRLDRNFA 420
           RKMSSLGNISYKS HSRQYS+SSLSENSRGSSEDPLI+PENSSECNESVVSSPRLDRNFA
Sbjct: 361 RKMSSLGNISYKSSHSRQYSLSSLSENSRGSSEDPLIDPENSSECNESVVSSPRLDRNFA 420

Query: 421 SIPKALSRGKSVRTIRANAIAIEEMKAQEEMYRNQVEHDDNIGNKFEGGMSPYMREDGIG 480
           + PKALSRGKSVRT+RA+  AIEEMKAQ EMYRNQVEHDDN+ NKFEGGMSPYMRED  G
Sbjct: 421 NTPKALSRGKSVRTVRASTSAIEEMKAQ-EMYRNQVEHDDNVENKFEGGMSPYMREDETG 480

Query: 481 HGWSGVVNPNAGNSNRFSK----MAFSGIEEQKEDTESQLTDDGKDNSEREDESLFASSD 540
           HGW G+ N NA  SNR+SK      FSGIEEQKEDTESQ+TDDGKDNSERED+S F SSD
Sbjct: 481 HGWPGINNLNAAYSNRYSKTTATTTFSGIEEQKEDTESQVTDDGKDNSEREDDSFFESSD 540

Query: 541 EEAALSMAGDSESGAHEVDKKAGEFIAKFREQIQLQRMASVDKRLRGGWGSFSSTSSSYF 594
           EEAALSM GDSESGAHEVDKKAGEFIAKFREQIQLQRMASVDKRLRGGWGSFSST+SSYF
Sbjct: 541 EEAALSMTGDSESGAHEVDKKAGEFIAKFREQIQLQRMASVDKRLRGGWGSFSSTTSSYF 600

BLAST of Lsi09G016780 vs. ExPASy TrEMBL
Match: A0A5D3DMA5 (DUF761 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G002840 PE=4 SV=1)

HSP 1 Score: 971.5 bits (2510), Expect = 1.6e-279
Identity = 525/600 (87.50%), Postives = 546/600 (91.00%), Query Frame = 0

Query: 1   MASSTSSPFTKPHFPHSPLPSISTTHHSKSCTQFLCKSLFFCIFLLLLPLFPSEAPEFVN 60
           MASS S+PFTKPHFPHSPLP  STT HS SCT FLCKSLFFCIFLLLLPLFPSEAPEFVN
Sbjct: 1   MASSPSTPFTKPHFPHSPLPPTSTTRHSNSCTHFLCKSLFFCIFLLLLPLFPSEAPEFVN 60

Query: 61  QTLLTKFWELFHLLFVGIAVSYGLFSRRNIQVSV--EEPRFSNFENPQSYLSKMFQVASI 120
           QTLLTKFWELFHL+FVGIAVSYGLFSRRN+QVSV  +EPRFSNFENPQSYLSKM  VASI
Sbjct: 61  QTLLTKFWELFHLMFVGIAVSYGLFSRRNVQVSVDSDEPRFSNFENPQSYLSKMLHVASI 120

Query: 121 FEDVDDLSVSDERKLSEVLYIQPNLGSVSDFNAQSHELEKYRYSIPKKRYENSHEFADTD 180
           FEDVDD SVSDERKLSEVLYIQPNLGSV  FNA S + E + YSIPKKRYENS EF DT+
Sbjct: 121 FEDVDDFSVSDERKLSEVLYIQPNLGSVRGFNAISRQQENFHYSIPKKRYENSLEFDDTN 180

Query: 181 NVGHVCKSRYTRGGSVVVVTETNRS-SGEWLESGAIVNYKPLGLPVRSLRSSLTEPDDVE 240
           +VGH CKSRYTRGGSVVVV ETNRS SGEWLESGAIVNYKPLGLPVRSLRS+LTEPDDVE
Sbjct: 181 SVGHACKSRYTRGGSVVVVAETNRSNSGEWLESGAIVNYKPLGLPVRSLRSNLTEPDDVE 240

Query: 241 FDCNDESCLSSKSSSKSSENNCER-SEFGDNCCVNLEEKFDETVIASMSPFQLREKFGKK 300
           FDC DESCLSSKSSSK+SE+NCER SEFGDNCCVNLEEKFDETVIA MSPFQLRE FGK 
Sbjct: 241 FDCGDESCLSSKSSSKNSESNCERTSEFGDNCCVNLEEKFDETVIAKMSPFQLRENFGKN 300

Query: 301 MMRERGVRNAVLRPSHFRPPSIDETQFESLKKSSSFHSNLSQSSQTSSLSSSLSSTTRKH 360
           MMRERGV+NAVLRPSHFRP SIDETQFESLKKS S HSNLSQSSQTSSLS SLSSTTRKH
Sbjct: 301 MMRERGVKNAVLRPSHFRPSSIDETQFESLKKSRSLHSNLSQSSQTSSLSPSLSSTTRKH 360

Query: 361 RKMSSLGNISYKSLHSRQYSMSSLSENSRGSSEDPLIEPENSSECNESVVSSPRLDRNFA 420
           RKMSSLGNISYKS HSRQYS+SSLSENSRGSSEDPLIEPENSSECNES++SSPRLDRNFA
Sbjct: 361 RKMSSLGNISYKSSHSRQYSLSSLSENSRGSSEDPLIEPENSSECNESIISSPRLDRNFA 420

Query: 421 SIPKALSRGKSVRTIRANAIAIEEMKAQEEMYRNQVEHDDNIGNKFEGGMSPYMREDGIG 480
            IPKALSRGKSVRTIRAN  AIEEMKAQ EMYRNQVEHDDN+GNKFEGGMSPYMREDG G
Sbjct: 421 HIPKALSRGKSVRTIRANTSAIEEMKAQ-EMYRNQVEHDDNVGNKFEGGMSPYMREDGTG 480

Query: 481 HGWSGVVNPNAGNSNRFSK-MAFSGIEEQKEDTESQLTDDG--KDNSEREDESLFASSDE 540
           HGW G+ +PNAG SNR  K   FSGIEEQKED ESQLTDD   +DNSERED S F SSDE
Sbjct: 481 HGWPGINSPNAGYSNRHPKTTTFSGIEEQKEDIESQLTDDDGKEDNSEREDVSFFESSDE 540

Query: 541 EAALSMAGDSESGAHEVDKKAGEFIAKFREQIQLQRMASVDKRLRGGWGSFSSTSSSYFS 594
           EAA SMAG+SESGA+EVDKKAGEFIAKFREQIQLQRMASVDKRLRGGWGSFSSTSSSYFS
Sbjct: 541 EAASSMAGESESGAYEVDKKAGEFIAKFREQIQLQRMASVDKRLRGGWGSFSSTSSSYFS 599

BLAST of Lsi09G016780 vs. ExPASy TrEMBL
Match: E5GCN2 (Uncharacterized protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 971.5 bits (2510), Expect = 1.6e-279
Identity = 525/600 (87.50%), Postives = 546/600 (91.00%), Query Frame = 0

Query: 1   MASSTSSPFTKPHFPHSPLPSISTTHHSKSCTQFLCKSLFFCIFLLLLPLFPSEAPEFVN 60
           MASS S+PFTKPHFPHSPLP  STT HS SCT FLCKSLFFCIFLLLLPLFPSEAPEFVN
Sbjct: 1   MASSPSTPFTKPHFPHSPLPPTSTTRHSNSCTHFLCKSLFFCIFLLLLPLFPSEAPEFVN 60

Query: 61  QTLLTKFWELFHLLFVGIAVSYGLFSRRNIQVSV--EEPRFSNFENPQSYLSKMFQVASI 120
           QTLLTKFWELFHL+FVGIAVSYGLFSRRN+QVSV  +EPRFSNFENPQSYLSKM  VASI
Sbjct: 61  QTLLTKFWELFHLMFVGIAVSYGLFSRRNVQVSVDSDEPRFSNFENPQSYLSKMLHVASI 120

Query: 121 FEDVDDLSVSDERKLSEVLYIQPNLGSVSDFNAQSHELEKYRYSIPKKRYENSHEFADTD 180
           FEDVDD SVSDERKLSEVLYIQPNLGSV  FNA S + E + YSIPKKRYENS EF DT+
Sbjct: 121 FEDVDDFSVSDERKLSEVLYIQPNLGSVRGFNAISRQQENFHYSIPKKRYENSLEFDDTN 180

Query: 181 NVGHVCKSRYTRGGSVVVVTETNRS-SGEWLESGAIVNYKPLGLPVRSLRSSLTEPDDVE 240
           +VGH CKSRYTRGGSVVVV ETNRS SGEWLESGAIVNYKPLGLPVRSLRS+LTEPDDVE
Sbjct: 181 SVGHACKSRYTRGGSVVVVAETNRSNSGEWLESGAIVNYKPLGLPVRSLRSNLTEPDDVE 240

Query: 241 FDCNDESCLSSKSSSKSSENNCER-SEFGDNCCVNLEEKFDETVIASMSPFQLREKFGKK 300
           FDC DESCLSSKSSSK+SE+NCER SEFGDNCCVNLEEKFDETVIA MSPFQLRE FGK 
Sbjct: 241 FDCGDESCLSSKSSSKNSESNCERTSEFGDNCCVNLEEKFDETVIAKMSPFQLRENFGKN 300

Query: 301 MMRERGVRNAVLRPSHFRPPSIDETQFESLKKSSSFHSNLSQSSQTSSLSSSLSSTTRKH 360
           MMRERGV+NAVLRPSHFRP SIDETQFESLKKS S HSNLSQSSQTSSLS SLSSTTRKH
Sbjct: 301 MMRERGVKNAVLRPSHFRPSSIDETQFESLKKSRSLHSNLSQSSQTSSLSPSLSSTTRKH 360

Query: 361 RKMSSLGNISYKSLHSRQYSMSSLSENSRGSSEDPLIEPENSSECNESVVSSPRLDRNFA 420
           RKMSSLGNISYKS HSRQYS+SSLSENSRGSSEDPLIEPENSSECNES++SSPRLDRNFA
Sbjct: 361 RKMSSLGNISYKSSHSRQYSLSSLSENSRGSSEDPLIEPENSSECNESIISSPRLDRNFA 420

Query: 421 SIPKALSRGKSVRTIRANAIAIEEMKAQEEMYRNQVEHDDNIGNKFEGGMSPYMREDGIG 480
            IPKALSRGKSVRTIRAN  AIEEMKAQ EMYRNQVEHDDN+GNKFEGGMSPYMREDG G
Sbjct: 421 HIPKALSRGKSVRTIRANTSAIEEMKAQ-EMYRNQVEHDDNVGNKFEGGMSPYMREDGTG 480

Query: 481 HGWSGVVNPNAGNSNRFSK-MAFSGIEEQKEDTESQLTDDG--KDNSEREDESLFASSDE 540
           HGW G+ +PNAG SNR  K   FSGIEEQKED ESQLTDD   +DNSERED S F SSDE
Sbjct: 481 HGWPGINSPNAGYSNRHPKTTTFSGIEEQKEDIESQLTDDDGKEDNSEREDVSFFESSDE 540

Query: 541 EAALSMAGDSESGAHEVDKKAGEFIAKFREQIQLQRMASVDKRLRGGWGSFSSTSSSYFS 594
           EAA SMAG+SESGA+EVDKKAGEFIAKFREQIQLQRMASVDKRLRGGWGSFSSTSSSYFS
Sbjct: 541 EAASSMAGESESGAYEVDKKAGEFIAKFREQIQLQRMASVDKRLRGGWGSFSSTSSSYFS 599

BLAST of Lsi09G016780 vs. ExPASy TrEMBL
Match: A0A6J1KUS4 (uncharacterized protein LOC111498900 OS=Cucurbita maxima OX=3661 GN=LOC111498900 PE=4 SV=1)

HSP 1 Score: 907.9 bits (2345), Expect = 2.2e-260
Identity = 495/597 (82.91%), Postives = 523/597 (87.60%), Query Frame = 0

Query: 1   MASSTSSPFTKPHFPHSPLPSISTTHHSKSCTQFLCKSLFFCIFLLLLPLFPSEAPEFVN 60
           MASS SSPFTK HFPHSPLP    THHS SC QFLCKSLFFC FLLLLPLFPSEAP+FV+
Sbjct: 1   MASSASSPFTKLHFPHSPLPQPPATHHSNSCAQFLCKSLFFCFFLLLLPLFPSEAPDFVD 60

Query: 61  QTLLTKFWELFHLLFVGIAVSYGLFSRRNIQVSVEEPRFSNFENPQSYLSKMFQVASIFE 120
           QTL TKFWELFHL+ VGIAVSYGLFS RN Q++V+EPR+S+FENPQSYLSKM  VASIF+
Sbjct: 61  QTLFTKFWELFHLMLVGIAVSYGLFSTRNNQMNVDEPRYSSFENPQSYLSKMLYVASIFD 120

Query: 121 DVDDLSVSDERKLSEVLYIQPNLGSVSDFNAQSHELEKYRYSIPKKRYENSHEFADTDNV 180
           DVDD SVSDERKLSEVLYIQPNLGS SD NAQS + EK RYSIPKKRYENS+EFADTDNV
Sbjct: 121 DVDDFSVSDERKLSEVLYIQPNLGSASDLNAQSRQQEKLRYSIPKKRYENSYEFADTDNV 180

Query: 181 GHVCKSRYTRGGSVVVVTETNRSSGEWLESGAIVNYKPLGLPVRSLRSSLTEPDDVEFDC 240
            H CKSRYTRGGSVVVV ETNRSS     SG IVNYKPLGLPVRSL+SSLTE DDVEFDC
Sbjct: 181 AHACKSRYTRGGSVVVVPETNRSS-----SGGIVNYKPLGLPVRSLKSSLTESDDVEFDC 240

Query: 241 NDESCLSSKSSSKSSENNCE-RSEFGDNCCVNLEEKFDETVIASMSPFQLREKFGKKMMR 300
            DESCLSSKSS KSSENNCE  SEFGDNCCVNLEEKFDET IASMS FQLREKFGKK++R
Sbjct: 241 GDESCLSSKSSPKSSENNCEGNSEFGDNCCVNLEEKFDETAIASMSSFQLREKFGKKVIR 300

Query: 301 ERGVRNAVLRPSHFRPPSIDETQFESLKKSSSFHSNLSQSSQTSSLSSSLSSTTRKHRKM 360
           ERG  NAVLRPSHFRPPSIDETQFESLKKS S HSNLSQSSQTSSLSSSLSSTTRKH KM
Sbjct: 301 ERGFGNAVLRPSHFRPPSIDETQFESLKKSGSLHSNLSQSSQTSSLSSSLSSTTRKHHKM 360

Query: 361 SSLGNISYKSLHSRQYSMSSLSENSRGSSEDPLIEPENSSECNESVVSSPRLDRNFASIP 420
           SSL NISYKSLHSRQYSMSSLSENSRGSSEDPLIE ENSSECNESVVSSPR D NF SIP
Sbjct: 361 SSLSNISYKSLHSRQYSMSSLSENSRGSSEDPLIEQENSSECNESVVSSPRSDMNFRSIP 420

Query: 421 KALSRGKSVRTIRANAIAIEEMKAQEEMYRNQVEHDDNIGNKF-EGGMSPYMREDGIGHG 480
           KALS+GKS+R I+ANA AIE++KAQ EM+R QV+HDD IGNKF EGG SPY+REDG GHG
Sbjct: 421 KALSQGKSIRRIQANAAAIEDIKAQ-EMHRKQVKHDDIIGNKFEEGGTSPYIREDGTGHG 480

Query: 481 WSGVVNPNAGNSNRFSKMAFSGIEEQKEDTESQLTDDGKDNSEREDESLFASSDEEAALS 540
           W  V NPNA N +RF    F GI+EQKE+TES + DD KD+SE EDES FASSDEEAA S
Sbjct: 481 WPDVANPNASNMSRFPTTTFLGIKEQKEETESLVADDSKDDSEGEDESFFASSDEEAASS 540

Query: 541 MAGDSESGAHEVDKKAGEFIAKFREQIQLQRMASVDKRLR--GGWGSFSSTSSSYFS 594
           MAGDSESGA EVDKKAGEFIAKFREQIQLQRMASV+KRLR  GGWGSFSSTSSSYFS
Sbjct: 541 MAGDSESGAFEVDKKAGEFIAKFREQIQLQRMASVEKRLRGGGGWGSFSSTSSSYFS 591

BLAST of Lsi09G016780 vs. ExPASy TrEMBL
Match: A0A6J1H4M0 (uncharacterized protein LOC111459998 OS=Cucurbita moschata OX=3662 GN=LOC111459998 PE=4 SV=1)

HSP 1 Score: 890.2 bits (2299), Expect = 4.8e-255
Identity = 495/602 (82.23%), Postives = 520/602 (86.38%), Query Frame = 0

Query: 1   MASSTSSPFTKPHFPHSPLPSISTTHHSKSCTQFLCKSLFFCIFLLLLPLFPSEAPEFVN 60
           MASS SSPFTK HFPHSPLP       + SC QFLCKS+FFC FLLLLPLFPSEAP+FV+
Sbjct: 1   MASSASSPFTKLHFPHSPLPQ----PPANSCAQFLCKSIFFCFFLLLLPLFPSEAPDFVD 60

Query: 61  QTLLTKFWELFHLLFVGIAVSYGLFSRRNIQVSVEEPRFSNFENPQSYLSKMFQVASIFE 120
           QTL TKFWELFHL+FVGIAVSYGLFS RN Q++V+EPR+S+FENPQSYLSKM  VASIF+
Sbjct: 61  QTLFTKFWELFHLMFVGIAVSYGLFSTRNNQMNVDEPRYSSFENPQSYLSKMLYVASIFD 120

Query: 121 DVDDLSVSDERKLSEVLYIQPNLGSVSDFNAQSHELEKYRYSIPKKRYENSHEFADTDNV 180
           DVDD  VSDERK+SEVLYIQP LGS SD NAQS   EK RYS+PKKRYENS+EFADTDNV
Sbjct: 121 DVDDFGVSDERKVSEVLYIQPKLGSASDLNAQSRHQEKLRYSMPKKRYENSYEFADTDNV 180

Query: 181 GHVCKSRYTRGGSVVVVTETNRSSGEWLESGAIVNYKPLGLPVRSLRSSLTEPDDVEFDC 240
            H CKSRYTRGGSVVVV ETNRSS     SG IVNYKPLGLPVRSLRSSLTE DDVEFDC
Sbjct: 181 AHACKSRYTRGGSVVVVPETNRSS-----SGGIVNYKPLGLPVRSLRSSLTESDDVEFDC 240

Query: 241 NDESCLSSKSSSKSSENNCE-RSEFGDNCCVNLEEKFDETVIASMSPFQLREKFGKKMMR 300
            DESCLSSKSS KSSENNCE  SEFGDNCCVNLEEKFDET IASMS FQLREKFGKK++R
Sbjct: 241 GDESCLSSKSSPKSSENNCEGNSEFGDNCCVNLEEKFDETAIASMSSFQLREKFGKKVIR 300

Query: 301 ERGVRNAVLRPSHFRPPSIDETQFESLKKSSSFHSNLSQSSQTSSLSSSLSSTTRKHRKM 360
           ERG  NAVLRPSHFRPPSIDETQFESLKKS S HS LSQSSQTSSLSS LSSTTRK RKM
Sbjct: 301 ERGFGNAVLRPSHFRPPSIDETQFESLKKSGSLHSYLSQSSQTSSLSSPLSSTTRKPRKM 360

Query: 361 SSLGNISYKSLHSRQYSMSSLSENSRGSSEDPLIEPENSSECNESVVSSPRLDRNFASIP 420
           SSL NISYKSLHSRQYS SSLSENSRGSSEDPLIE ENSSECNESVVSSPR DRNFASIP
Sbjct: 361 SSLSNISYKSLHSRQYSTSSLSENSRGSSEDPLIEQENSSECNESVVSSPRSDRNFASIP 420

Query: 421 KALSRGKSVRTIRANAIAIEEMKAQEEMYRNQVEHDDNIGNKF-EGGMS-PYMREDGIGH 480
           KALS+GKSVR IRANA AIE+MKAQ EM+R QV+HDD IGNKF EGGMS PYMREDG G 
Sbjct: 421 KALSQGKSVRRIRANAAAIEDMKAQ-EMHRKQVKHDDIIGNKFEEGGMSPPYMREDGTGQ 480

Query: 481 GWSGVVNPNAGNSNRFSKMAFSGIEEQKEDTESQLTDDGKDNSEREDESLFASSDEEAAL 540
           GW  VVNPNAGN NRF K  F GI+EQKE+TES + DD KD+SE EDESLFASSDEEA  
Sbjct: 481 GWPDVVNPNAGNMNRFPKTTFLGIKEQKEETESLVADDSKDDSEGEDESLFASSDEEAGS 540

Query: 541 SMAGDSESGAHEVDKKAGEFIAKFREQIQLQRMASVDKRLR------GGWGSFSSTSSSY 594
           SMAGDSESGA EVDKKAGEFIAKFREQIQLQRMASV+KRLR      GGWGSFSSTSSSY
Sbjct: 541 SMAGDSESGAFEVDKKAGEFIAKFREQIQLQRMASVEKRLRGGGGGGGGWGSFSSTSSSY 592

BLAST of Lsi09G016780 vs. NCBI nr
Match: XP_004140631.1 (uncharacterized protein LOC101220435 [Cucumis sativus] >KGN46495.1 hypothetical protein Csa_004883 [Cucumis sativus])

HSP 1 Score: 974.5 bits (2518), Expect = 4.0e-280
Identity = 523/601 (87.02%), Postives = 548/601 (91.18%), Query Frame = 0

Query: 1   MASSTSSPFTKPHFPHSPLPSISTTHHSKSCTQFLCKSLFFCIFLLLLPLFPSEAPEFVN 60
           MA S S+PFTKPHFPHSPLP  STT HS SCTQF+CKSLFFCIFLLLLPLFPSEAPEFVN
Sbjct: 1   MAPSPSTPFTKPHFPHSPLPPTSTTRHSNSCTQFICKSLFFCIFLLLLPLFPSEAPEFVN 60

Query: 61  QTLLTKFWELFHLLFVGIAVSYGLFSRRNIQVSV--EEPRFSNFENPQSYLSKMFQVASI 120
           QT LTKFWELFHL+F+GIAVSYGLFSRRN+QVSV  +EPRFSNFENPQSYLSKMF VASI
Sbjct: 61  QTFLTKFWELFHLMFIGIAVSYGLFSRRNVQVSVDSDEPRFSNFENPQSYLSKMFHVASI 120

Query: 121 FEDVDDLSVSDERKLSEVLYIQPNLGSVSDFNAQSHELEKYRYSIPKKRYENSHEFADTD 180
           FEDVDD SVSDERKLSEVLYIQPNLGSVS  NA S + E + YSIPKKRYENS EFA+TD
Sbjct: 121 FEDVDDFSVSDERKLSEVLYIQPNLGSVSGLNAISRQQENFHYSIPKKRYENSLEFAETD 180

Query: 181 NVGHVCKSRYTRGGSVVVVTETNRS-SGEWLESGAIVNYKPLGLPVRSLRSSLTEPDDVE 240
           NVGH CKSRYTRGGSVVVV ETNRS SGEWLESGAIVNYKPLGLPVRSL+SSLTEPDDVE
Sbjct: 181 NVGHACKSRYTRGGSVVVVAETNRSNSGEWLESGAIVNYKPLGLPVRSLKSSLTEPDDVE 240

Query: 241 FDCNDESCLSSKSSSKSSENNCER-SEFGDNCCVNLEEKFDETVIASMSPFQLREKFGKK 300
           FDC DESCLSSKSSSK+SE+NCER SEFGDNCCVNLEEKFDETVIASMSPFQLREKF K 
Sbjct: 241 FDCGDESCLSSKSSSKNSESNCERTSEFGDNCCVNLEEKFDETVIASMSPFQLREKFEKN 300

Query: 301 MMRERGVRNAVLRPSHFRPPSIDETQFESLKKSSSFHSNLSQSSQTSSLSSSLSSTTRKH 360
           MMRER V+NAVLRPSHFRP SIDETQFESLKKS+S HSNLSQSSQTSSLSS LSS TRKH
Sbjct: 301 MMRERRVKNAVLRPSHFRPSSIDETQFESLKKSTSLHSNLSQSSQTSSLSSPLSSRTRKH 360

Query: 361 RKMSSLGNISYKSLHSRQYSMSSLSENSRGSSEDPLIEPENSSECNESVVSSPRLDRNFA 420
           RKMSSLGNISYKS HSRQYS+SSLSENSRGSSEDPLI+PENSSECNESVVSSPRLDRNFA
Sbjct: 361 RKMSSLGNISYKSSHSRQYSLSSLSENSRGSSEDPLIDPENSSECNESVVSSPRLDRNFA 420

Query: 421 SIPKALSRGKSVRTIRANAIAIEEMKAQEEMYRNQVEHDDNIGNKFEGGMSPYMREDGIG 480
           + PKALSRGKSVRT+RA+  AIEEMKAQ EMYRNQVEHDDN+ NKFEGGMSPYMRED  G
Sbjct: 421 NTPKALSRGKSVRTVRASTSAIEEMKAQ-EMYRNQVEHDDNVENKFEGGMSPYMREDETG 480

Query: 481 HGWSGVVNPNAGNSNRFSK----MAFSGIEEQKEDTESQLTDDGKDNSEREDESLFASSD 540
           HGW G+ N NA  SNR+SK      FSGIEEQKEDTESQ+TDDGKDNSERED+S F SSD
Sbjct: 481 HGWPGINNLNAAYSNRYSKTTATTTFSGIEEQKEDTESQVTDDGKDNSEREDDSFFESSD 540

Query: 541 EEAALSMAGDSESGAHEVDKKAGEFIAKFREQIQLQRMASVDKRLRGGWGSFSSTSSSYF 594
           EEAALSM GDSESGAHEVDKKAGEFIAKFREQIQLQRMASVDKRLRGGWGSFSST+SSYF
Sbjct: 541 EEAALSMTGDSESGAHEVDKKAGEFIAKFREQIQLQRMASVDKRLRGGWGSFSSTTSSYF 600

BLAST of Lsi09G016780 vs. NCBI nr
Match: ADN34231.1 (hypothetical protein [Cucumis melo subsp. melo] >TYK24724.1 DUF761 domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 971.5 bits (2510), Expect = 3.4e-279
Identity = 525/600 (87.50%), Postives = 546/600 (91.00%), Query Frame = 0

Query: 1   MASSTSSPFTKPHFPHSPLPSISTTHHSKSCTQFLCKSLFFCIFLLLLPLFPSEAPEFVN 60
           MASS S+PFTKPHFPHSPLP  STT HS SCT FLCKSLFFCIFLLLLPLFPSEAPEFVN
Sbjct: 1   MASSPSTPFTKPHFPHSPLPPTSTTRHSNSCTHFLCKSLFFCIFLLLLPLFPSEAPEFVN 60

Query: 61  QTLLTKFWELFHLLFVGIAVSYGLFSRRNIQVSV--EEPRFSNFENPQSYLSKMFQVASI 120
           QTLLTKFWELFHL+FVGIAVSYGLFSRRN+QVSV  +EPRFSNFENPQSYLSKM  VASI
Sbjct: 61  QTLLTKFWELFHLMFVGIAVSYGLFSRRNVQVSVDSDEPRFSNFENPQSYLSKMLHVASI 120

Query: 121 FEDVDDLSVSDERKLSEVLYIQPNLGSVSDFNAQSHELEKYRYSIPKKRYENSHEFADTD 180
           FEDVDD SVSDERKLSEVLYIQPNLGSV  FNA S + E + YSIPKKRYENS EF DT+
Sbjct: 121 FEDVDDFSVSDERKLSEVLYIQPNLGSVRGFNAISRQQENFHYSIPKKRYENSLEFDDTN 180

Query: 181 NVGHVCKSRYTRGGSVVVVTETNRS-SGEWLESGAIVNYKPLGLPVRSLRSSLTEPDDVE 240
           +VGH CKSRYTRGGSVVVV ETNRS SGEWLESGAIVNYKPLGLPVRSLRS+LTEPDDVE
Sbjct: 181 SVGHACKSRYTRGGSVVVVAETNRSNSGEWLESGAIVNYKPLGLPVRSLRSNLTEPDDVE 240

Query: 241 FDCNDESCLSSKSSSKSSENNCER-SEFGDNCCVNLEEKFDETVIASMSPFQLREKFGKK 300
           FDC DESCLSSKSSSK+SE+NCER SEFGDNCCVNLEEKFDETVIA MSPFQLRE FGK 
Sbjct: 241 FDCGDESCLSSKSSSKNSESNCERTSEFGDNCCVNLEEKFDETVIAKMSPFQLRENFGKN 300

Query: 301 MMRERGVRNAVLRPSHFRPPSIDETQFESLKKSSSFHSNLSQSSQTSSLSSSLSSTTRKH 360
           MMRERGV+NAVLRPSHFRP SIDETQFESLKKS S HSNLSQSSQTSSLS SLSSTTRKH
Sbjct: 301 MMRERGVKNAVLRPSHFRPSSIDETQFESLKKSRSLHSNLSQSSQTSSLSPSLSSTTRKH 360

Query: 361 RKMSSLGNISYKSLHSRQYSMSSLSENSRGSSEDPLIEPENSSECNESVVSSPRLDRNFA 420
           RKMSSLGNISYKS HSRQYS+SSLSENSRGSSEDPLIEPENSSECNES++SSPRLDRNFA
Sbjct: 361 RKMSSLGNISYKSSHSRQYSLSSLSENSRGSSEDPLIEPENSSECNESIISSPRLDRNFA 420

Query: 421 SIPKALSRGKSVRTIRANAIAIEEMKAQEEMYRNQVEHDDNIGNKFEGGMSPYMREDGIG 480
            IPKALSRGKSVRTIRAN  AIEEMKAQ EMYRNQVEHDDN+GNKFEGGMSPYMREDG G
Sbjct: 421 HIPKALSRGKSVRTIRANTSAIEEMKAQ-EMYRNQVEHDDNVGNKFEGGMSPYMREDGTG 480

Query: 481 HGWSGVVNPNAGNSNRFSK-MAFSGIEEQKEDTESQLTDDG--KDNSEREDESLFASSDE 540
           HGW G+ +PNAG SNR  K   FSGIEEQKED ESQLTDD   +DNSERED S F SSDE
Sbjct: 481 HGWPGINSPNAGYSNRHPKTTTFSGIEEQKEDIESQLTDDDGKEDNSEREDVSFFESSDE 540

Query: 541 EAALSMAGDSESGAHEVDKKAGEFIAKFREQIQLQRMASVDKRLRGGWGSFSSTSSSYFS 594
           EAA SMAG+SESGA+EVDKKAGEFIAKFREQIQLQRMASVDKRLRGGWGSFSSTSSSYFS
Sbjct: 541 EAASSMAGESESGAYEVDKKAGEFIAKFREQIQLQRMASVDKRLRGGWGSFSSTSSSYFS 599

BLAST of Lsi09G016780 vs. NCBI nr
Match: XP_023006022.1 (uncharacterized protein LOC111498900 [Cucurbita maxima])

HSP 1 Score: 907.9 bits (2345), Expect = 4.6e-260
Identity = 495/597 (82.91%), Postives = 523/597 (87.60%), Query Frame = 0

Query: 1   MASSTSSPFTKPHFPHSPLPSISTTHHSKSCTQFLCKSLFFCIFLLLLPLFPSEAPEFVN 60
           MASS SSPFTK HFPHSPLP    THHS SC QFLCKSLFFC FLLLLPLFPSEAP+FV+
Sbjct: 1   MASSASSPFTKLHFPHSPLPQPPATHHSNSCAQFLCKSLFFCFFLLLLPLFPSEAPDFVD 60

Query: 61  QTLLTKFWELFHLLFVGIAVSYGLFSRRNIQVSVEEPRFSNFENPQSYLSKMFQVASIFE 120
           QTL TKFWELFHL+ VGIAVSYGLFS RN Q++V+EPR+S+FENPQSYLSKM  VASIF+
Sbjct: 61  QTLFTKFWELFHLMLVGIAVSYGLFSTRNNQMNVDEPRYSSFENPQSYLSKMLYVASIFD 120

Query: 121 DVDDLSVSDERKLSEVLYIQPNLGSVSDFNAQSHELEKYRYSIPKKRYENSHEFADTDNV 180
           DVDD SVSDERKLSEVLYIQPNLGS SD NAQS + EK RYSIPKKRYENS+EFADTDNV
Sbjct: 121 DVDDFSVSDERKLSEVLYIQPNLGSASDLNAQSRQQEKLRYSIPKKRYENSYEFADTDNV 180

Query: 181 GHVCKSRYTRGGSVVVVTETNRSSGEWLESGAIVNYKPLGLPVRSLRSSLTEPDDVEFDC 240
            H CKSRYTRGGSVVVV ETNRSS     SG IVNYKPLGLPVRSL+SSLTE DDVEFDC
Sbjct: 181 AHACKSRYTRGGSVVVVPETNRSS-----SGGIVNYKPLGLPVRSLKSSLTESDDVEFDC 240

Query: 241 NDESCLSSKSSSKSSENNCE-RSEFGDNCCVNLEEKFDETVIASMSPFQLREKFGKKMMR 300
            DESCLSSKSS KSSENNCE  SEFGDNCCVNLEEKFDET IASMS FQLREKFGKK++R
Sbjct: 241 GDESCLSSKSSPKSSENNCEGNSEFGDNCCVNLEEKFDETAIASMSSFQLREKFGKKVIR 300

Query: 301 ERGVRNAVLRPSHFRPPSIDETQFESLKKSSSFHSNLSQSSQTSSLSSSLSSTTRKHRKM 360
           ERG  NAVLRPSHFRPPSIDETQFESLKKS S HSNLSQSSQTSSLSSSLSSTTRKH KM
Sbjct: 301 ERGFGNAVLRPSHFRPPSIDETQFESLKKSGSLHSNLSQSSQTSSLSSSLSSTTRKHHKM 360

Query: 361 SSLGNISYKSLHSRQYSMSSLSENSRGSSEDPLIEPENSSECNESVVSSPRLDRNFASIP 420
           SSL NISYKSLHSRQYSMSSLSENSRGSSEDPLIE ENSSECNESVVSSPR D NF SIP
Sbjct: 361 SSLSNISYKSLHSRQYSMSSLSENSRGSSEDPLIEQENSSECNESVVSSPRSDMNFRSIP 420

Query: 421 KALSRGKSVRTIRANAIAIEEMKAQEEMYRNQVEHDDNIGNKF-EGGMSPYMREDGIGHG 480
           KALS+GKS+R I+ANA AIE++KAQ EM+R QV+HDD IGNKF EGG SPY+REDG GHG
Sbjct: 421 KALSQGKSIRRIQANAAAIEDIKAQ-EMHRKQVKHDDIIGNKFEEGGTSPYIREDGTGHG 480

Query: 481 WSGVVNPNAGNSNRFSKMAFSGIEEQKEDTESQLTDDGKDNSEREDESLFASSDEEAALS 540
           W  V NPNA N +RF    F GI+EQKE+TES + DD KD+SE EDES FASSDEEAA S
Sbjct: 481 WPDVANPNASNMSRFPTTTFLGIKEQKEETESLVADDSKDDSEGEDESFFASSDEEAASS 540

Query: 541 MAGDSESGAHEVDKKAGEFIAKFREQIQLQRMASVDKRLR--GGWGSFSSTSSSYFS 594
           MAGDSESGA EVDKKAGEFIAKFREQIQLQRMASV+KRLR  GGWGSFSSTSSSYFS
Sbjct: 541 MAGDSESGAFEVDKKAGEFIAKFREQIQLQRMASVEKRLRGGGGWGSFSSTSSSYFS 591

BLAST of Lsi09G016780 vs. NCBI nr
Match: KAG6575261.1 (hypothetical protein SDJN03_25900, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 898.3 bits (2320), Expect = 3.6e-257
Identity = 496/599 (82.80%), Postives = 522/599 (87.15%), Query Frame = 0

Query: 1   MASSTSSPFTKPHFPHSPLPSISTTHHSKSCTQFLCKSLFFCIFLLLLPLFPSEAPEFVN 60
           MASS SSPFTK HFPHSPLP       + SC QFLCKS+FFC FLLLLPLFPSEAP+FV+
Sbjct: 1   MASSASSPFTKLHFPHSPLPQ----PPANSCAQFLCKSIFFCFFLLLLPLFPSEAPDFVD 60

Query: 61  QTLLTKFWELFHLLFVGIAVSYGLFSRRNIQVSVEEPRFSNFENPQSYLSKMFQVASIFE 120
           QTL TKFWELFHL+FVGIAVSYGLFS RN Q++V+EPR+S+FENPQSYLSKM  VASIF+
Sbjct: 61  QTLFTKFWELFHLMFVGIAVSYGLFSTRNNQMNVDEPRYSSFENPQSYLSKMLYVASIFD 120

Query: 121 DVDDLSVSDERKLSEVLYIQPNLGSVSDFNAQSHELEKYRYSIPKKRYENSHEFADTDNV 180
           DVDD  VSDERK+SEVLYIQP LGS SD NAQS   EK RYS+PKKRYENS+EFADTDNV
Sbjct: 121 DVDDFGVSDERKVSEVLYIQPKLGSASDLNAQSRHQEKLRYSMPKKRYENSYEFADTDNV 180

Query: 181 GHVCKSRYTRGGSVVVVTETNRSSGEWLESGAIVNYKPLGLPVRSLRSSLTEPDDVEFDC 240
            H CKSRYTRGGSVVVV ETNRSS     SG IVNYKPLGLPVRSLRSSLTE DDVEFDC
Sbjct: 181 AHACKSRYTRGGSVVVVPETNRSS-----SGGIVNYKPLGLPVRSLRSSLTESDDVEFDC 240

Query: 241 NDESCLSSKSSSKSSENNCE-RSEFGDNCCVNLEEKFDETVIASMSPFQLREKFGKKMMR 300
            DESCLSSKSS KSSENNCE  SEFGDNCCVNLEEKFDET IASMS FQLREKFGKK++R
Sbjct: 241 GDESCLSSKSSPKSSENNCEGNSEFGDNCCVNLEEKFDETAIASMSSFQLREKFGKKVIR 300

Query: 301 ERGVRNAVLRPSHFRPPSIDETQFESLKKSSSFHSNLSQSSQTSSLSSSLSSTTRKHRKM 360
           ERG  NAVLRPSHFRPPSIDETQFESL+KS S HS+LSQSSQTSSLSS LSSTTRKH KM
Sbjct: 301 ERGFGNAVLRPSHFRPPSIDETQFESLRKSGSLHSDLSQSSQTSSLSSQLSSTTRKHSKM 360

Query: 361 SSLGNISYKSLHSRQYSMSSLSENSRGSSEDPLIEPENSSECNESVVSSPRLDRNFASIP 420
           SSL NISYKSLHSRQYSMSSLSENSRGSSEDPLIE ENSSECNESVVSSPR DRNFASIP
Sbjct: 361 SSLSNISYKSLHSRQYSMSSLSENSRGSSEDPLIEQENSSECNESVVSSPRSDRNFASIP 420

Query: 421 KALSRGKSVRTIRANAIAIEEMKAQEEMYRNQVEHDDNIGNKF-EGGMS-PYMREDGIGH 480
           KALS+GKSVR IRANA AIE+MKAQ EM+R QV+HDD IGNKF EGGMS PYMREDG GH
Sbjct: 421 KALSQGKSVRRIRANAAAIEDMKAQ-EMHRKQVKHDDIIGNKFEEGGMSPPYMREDGTGH 480

Query: 481 GWSGVVNPNAGNSNRFSKMAFSGIEEQKEDTESQLTDDGKDNSEREDESLFASSDEEAAL 540
           GW  VVNPNAGN NRF K  F GI+EQKE+TES + DD KD SE EDESLFASSDEEA  
Sbjct: 481 GWPDVVNPNAGNMNRFPKTTFLGIKEQKEETESLVADDSKDGSEGEDESLFASSDEEAGS 540

Query: 541 SMAGDSESGAHEVDKKAGEFIAKFREQIQLQRMASVDKRLR---GGWGSFSSTSSSYFS 594
           SMAGDSESGA EVDKKAGEFIAKFREQIQLQRMASV+KRLR   GGWGSFSSTSSSYFS
Sbjct: 541 SMAGDSESGAFEVDKKAGEFIAKFREQIQLQRMASVEKRLRGGGGGWGSFSSTSSSYFS 589

BLAST of Lsi09G016780 vs. NCBI nr
Match: KAG7013816.1 (hypothetical protein SDJN02_23985, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 895.6 bits (2313), Expect = 2.3e-256
Identity = 495/599 (82.64%), Postives = 521/599 (86.98%), Query Frame = 0

Query: 1   MASSTSSPFTKPHFPHSPLPSISTTHHSKSCTQFLCKSLFFCIFLLLLPLFPSEAPEFVN 60
           MASS SSPFTK HFPHSPLP       + SC QFLCKS+FFC FLLLLPLFPSEAP+FV+
Sbjct: 1   MASSASSPFTKLHFPHSPLPQ----PPANSCAQFLCKSIFFCFFLLLLPLFPSEAPDFVD 60

Query: 61  QTLLTKFWELFHLLFVGIAVSYGLFSRRNIQVSVEEPRFSNFENPQSYLSKMFQVASIFE 120
           QTL TKFWELFHL+FVGIAVSYGLFS RN Q++V+EPR+S+FENPQSYLSKM  VASIF+
Sbjct: 61  QTLFTKFWELFHLMFVGIAVSYGLFSTRNNQMNVDEPRYSSFENPQSYLSKMLYVASIFD 120

Query: 121 DVDDLSVSDERKLSEVLYIQPNLGSVSDFNAQSHELEKYRYSIPKKRYENSHEFADTDNV 180
           DVDD  VSDERK+SEVLYIQP LGS SD NAQS   EK RYS+PKKRYENS+EFADTDNV
Sbjct: 121 DVDDFGVSDERKVSEVLYIQPKLGSASDLNAQSRHQEKLRYSMPKKRYENSYEFADTDNV 180

Query: 181 GHVCKSRYTRGGSVVVVTETNRSSGEWLESGAIVNYKPLGLPVRSLRSSLTEPDDVEFDC 240
            H CKSRYTRGGSVVVV ETNRSS     SG IVNYKPLGLPVRSLRSSLTE DDVEFDC
Sbjct: 181 AHACKSRYTRGGSVVVVPETNRSS-----SGGIVNYKPLGLPVRSLRSSLTESDDVEFDC 240

Query: 241 NDESCLSSKSSSKSSENNCE-RSEFGDNCCVNLEEKFDETVIASMSPFQLREKFGKKMMR 300
            DESCLSSKSS KSSENNCE  SEFGDNCCVNLEEKFDET IASMS FQLREKFGKK++R
Sbjct: 241 GDESCLSSKSSPKSSENNCEGNSEFGDNCCVNLEEKFDETAIASMSSFQLREKFGKKVIR 300

Query: 301 ERGVRNAVLRPSHFRPPSIDETQFESLKKSSSFHSNLSQSSQTSSLSSSLSSTTRKHRKM 360
           ERG  NAVLRPSHFRPPSIDETQFESL+KS S HS+LSQSSQTSSLSS LSSTTRKH KM
Sbjct: 301 ERGFGNAVLRPSHFRPPSIDETQFESLRKSGSLHSDLSQSSQTSSLSSPLSSTTRKHSKM 360

Query: 361 SSLGNISYKSLHSRQYSMSSLSENSRGSSEDPLIEPENSSECNESVVSSPRLDRNFASIP 420
           SSL NISYKSLHSRQYSMSSLSENSRGSSEDPLIE ENSSECNESVVSSPR DRNFASIP
Sbjct: 361 SSLSNISYKSLHSRQYSMSSLSENSRGSSEDPLIEQENSSECNESVVSSPRSDRNFASIP 420

Query: 421 KALSRGKSVRTIRANAIAIEEMKAQEEMYRNQVEHDDNIGNKF-EGGMS-PYMREDGIGH 480
           KALS+GKSVR IRANA AIE+MKAQ EM+R QV+HDD IGNKF EGGMS PYMREDG GH
Sbjct: 421 KALSQGKSVRRIRANAAAIEDMKAQ-EMHRKQVKHDDIIGNKFEEGGMSPPYMREDGTGH 480

Query: 481 GWSGVVNPNAGNSNRFSKMAFSGIEEQKEDTESQLTDDGKDNSEREDESLFASSDEEAAL 540
           GW  VVNPNAGN NRF K  F GI+EQKE+TES + DD KD SE EDES FASSDEEA  
Sbjct: 481 GWPDVVNPNAGNMNRFPKTTFLGIKEQKEETESLVADDSKDGSEGEDESSFASSDEEAGS 540

Query: 541 SMAGDSESGAHEVDKKAGEFIAKFREQIQLQRMASVDKRLR---GGWGSFSSTSSSYFS 594
           SMAGDSESGA EVDKKAGEFIAKFREQIQLQRMASV+KRLR   GGWGSFSSTSSSYFS
Sbjct: 541 SMAGDSESGAFEVDKKAGEFIAKFREQIQLQRMASVEKRLRGGGGGWGSFSSTSSSYFS 589

BLAST of Lsi09G016780 vs. TAIR 10
Match: AT3G60380.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: hydroxyproline-rich glycoprotein family protein (TAIR:AT4G16790.1); Has 6102 Blast hits to 3981 proteins in 424 species: Archae - 6; Bacteria - 372; Metazoa - 2603; Fungi - 655; Plants - 291; Viruses - 28; Other Eukaryotes - 2147 (source: NCBI BLink). )

HSP 1 Score: 144.4 bits (363), Expect = 2.8e-34
Identity = 141/399 (35.34%), Postives = 203/399 (50.88%), Query Frame = 0

Query: 4   STSSPFTKPHFPHSPLPSISTTHHSKSCTQFLCKSLFFCIFLLLLPLFPSEAPEFVNQTL 63
           ++ +P+TK   P + +      + S     F CKS+ F +FLL LPLFPS+AP+FV +T+
Sbjct: 2   ASPNPYTKRRSPPNVVVPPQPRYKSIGGGGFFCKSVLFALFLLALPLFPSQAPDFVGETV 61

Query: 64  LTKFWELFHLLFVGIAVSYGLFSRRNIQVSVEEPRFSNFENPQSYLSKMFQVASIF-EDV 123
           LTKFWEL HLLFVGIAV+YGLFSRRN++ +V+       E+  SY+S++FQV+S+F E+ 
Sbjct: 62  LTKFWELIHLLFVGIAVAYGLFSRRNVESAVDLRMTRVDESSLSYVSRIFQVSSVFDEEF 121

Query: 124 DDLSVS--DERKLSEVLYIQPNLGSVSDFNAQSHELEKYRYSIPKKRYENSHEFADTDNV 183
           DD S    D R    V      +G    F  +S ELE+            S EF +T+ V
Sbjct: 122 DDNSCEFVDVRSDESVSARASVVGKSESFVVESGELEE------------SSEFGETNEV 181

Query: 184 GHVCKSRYTRGGSVVVVTETNRSSGEWLESGAIVNYKPLGLPVRSLRSSLTEPDDVEFDC 243
                S+Y +G S VVV         +   G +V ++PLGLP+R LRSSL          
Sbjct: 182 -RAWNSQYFQGKSKVVVARP-----AYGLDGHVV-HQPLGLPIRRLRSSL---------- 241

Query: 244 NDESCLSSKSSSKSSENNCERSEFGDNCCVNLEEKFDETVIASMSPFQLREKFGKKMMRE 303
            D + L  KS + S    C+ +   +   +  +  FDE + A  SP   + +   +MM  
Sbjct: 242 RDNAALQDKSFADS----CDGAVNAEAESLLADNFFDEVLAAPASPVPWQAR--PEMM-- 301

Query: 304 RGVRNAVLRPSHFRPPSIDETQFESLKKSSSFHSNLSQSSQTSSLSSSLSSTTRKHRKMS 363
            G+ +    PS+F+P S+DET      KS S  S  S SSQTS  S       +   + S
Sbjct: 302 -GIGDNY--PSNFQPISVDET-----LKSISSRSTGSSSSQTSYAS-------QNQNRFS 348

Query: 364 SLGNISYKSLHSR-QYSMSSLSENSRGSSEDPLIEPENS 399
              ++S +SL+S  +  +   S  S   S  P + P  S
Sbjct: 362 PSRSVSAESLNSNVEELVKEKSRQSSSRSSSPSLPPSPS 348


HSP 2 Score: 43.9 bits (102), Expect = 5.2e-04
Identity = 32/85 (37.65%), Postives = 51/85 (60.00%), Query Frame = 0

Query: 503 EQKEDTESQLTDDGKDNSEREDESLFASSDEEAALSMAGDSESGAHEVDKKAGEFIAKFR 562
           E +  +E +     +  +E++ E  F   +EEAA     ++    +EVD+KAGEFIAKFR
Sbjct: 657 ETRAKSEPEEVAMEEPQAEQQPEVTF-EEEEEAAWESQSNASHDHNEVDRKAGEFIAKFR 716

Query: 563 EQIQLQRMASVDKRLRGGWGSFSST 588
           EQI+LQ++ S ++   GG G F ++
Sbjct: 717 EQIRLQKLISGEQPRGGGTGIFRNS 740

BLAST of Lsi09G016780 vs. TAIR 10
Match: AT4G16790.1 (hydroxyproline-rich glycoprotein family protein )

HSP 1 Score: 65.9 bits (159), Expect = 1.3e-10
Identity = 45/119 (37.82%), Postives = 63/119 (52.94%), Query Frame = 0

Query: 29  KSCTQFLCKSLFFCIFLLLLPLFPSEAPEFVNQTLLTKFWELFHLLFVGIAVSYGLFSRR 88
           K  ++F+ K+L   +   ++P+F S+ PE  NQT L    EL HL+FVGIAVSYGLFSRR
Sbjct: 23  KFYSRFIFKALILTVLCAVVPVFLSQTPELANQTRLL---ELLHLVFVGIAVSYGLFSRR 82

Query: 89  N--------IQVSVEEPRFSNFENPQSYLSKMFQVASIF-------EDVDDLSVSDERK 133
           N           S       +  N  SY+ K+ +V+S+F        +  D S  D+RK
Sbjct: 83  NYDGGGGGGTSNSDHNKADHSNNNSHSYVPKILEVSSVFNVGHESESEPSDDSSGDQRK 138


HSP 2 Score: 40.4 bits (93), Expect = 5.8e-03
Identity = 30/85 (35.29%), Postives = 49/85 (57.65%), Query Frame = 0

Query: 501 IEEQKEDTE---------SQLTDDGKDNSEREDESLFASSDEEAALSMAGDSE-SGAHEV 560
           +E +++DTE         S+  ++ ++  +R  E+      E+  +   G SE +   +V
Sbjct: 378 VESKEQDTEKNDQRSNLGSKAVEESENGEQRRGENEIHDEVEKKIVEEEGVSEINNGSDV 437

Query: 561 DKKAGEFIAKFREQIQLQRMASVDK 576
           DKKA EFIAKFREQI+LQR+ S+ +
Sbjct: 438 DKKADEFIAKFREQIRLQRIESIKR 462

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A0A0K9X11.9e-28087.02Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G103540 PE=4 SV=1[more]
A0A5D3DMA51.6e-27987.50DUF761 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
E5GCN21.6e-27987.50Uncharacterized protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1[more]
A0A6J1KUS42.2e-26082.91uncharacterized protein LOC111498900 OS=Cucurbita maxima OX=3661 GN=LOC111498900... [more]
A0A6J1H4M04.8e-25582.23uncharacterized protein LOC111459998 OS=Cucurbita moschata OX=3662 GN=LOC1114599... [more]
Match NameE-valueIdentityDescription
XP_004140631.14.0e-28087.02uncharacterized protein LOC101220435 [Cucumis sativus] >KGN46495.1 hypothetical ... [more]
ADN34231.13.4e-27987.50hypothetical protein [Cucumis melo subsp. melo] >TYK24724.1 DUF761 domain-contai... [more]
XP_023006022.14.6e-26082.91uncharacterized protein LOC111498900 [Cucurbita maxima][more]
KAG6575261.13.6e-25782.80hypothetical protein SDJN03_25900, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG7013816.12.3e-25682.64hypothetical protein SDJN02_23985, partial [Cucurbita argyrosperma subsp. argyro... [more]
Match NameE-valueIdentityDescription
AT3G60380.12.8e-3435.34FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow... [more]
AT4G16790.11.3e-1037.82hydroxyproline-rich glycoprotein family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008480Protein of unknown function DUF761, plantPFAMPF05553DUF761coord: 549..575
e-value: 6.0E-10
score: 38.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 501..526
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 332..408
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 501..551
NoneNo IPR availablePANTHERPTHR34059EXPRESSED PROTEINcoord: 365..581
NoneNo IPR availablePANTHERPTHR34059:SF6COTTON FIBER PROTEINcoord: 365..581
NoneNo IPR availablePANTHERPTHR34059:SF6COTTON FIBER PROTEINcoord: 4..360
NoneNo IPR availablePANTHERPTHR34059EXPRESSED PROTEINcoord: 4..360

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi09G016780.1Lsi09G016780.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane