Lsi09G012550 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi09G012550
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	serine/threonine-protein kinase BLUS1-like
Location	chr09: 20212599 .. 20214155 (-)
RNA-Seq Expression	Lsi09G012550
Synteny	Lsi09G012550

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGCACCATCAAGTTCAAGAAACCACCGCCACCTCCGCCGCTAGTACACTGAATTTCAACTTTCCGACGACCTCCGATGGCTACACAATCATTACTCAGATCGGTGCCAGCCCCCACAGCATTGTGTACATGGCCCATTGCAAGTCGGAAGACTCGACGACGATCACCGTCGCCGTAAAGATTATGGATATCAATATCAATCTCGACCTCAATTCCCTAAACGCTTCACTATCACTGATCAACCACCCCAATATTCTCCGGCCACATTGTTGTTTCGTCGGCGACGACGGCCGCATTTGGATCGTAATGCCCTTCATTCCAACCGGCCCTTTACAATCCGTTATTTCTCACAGTTTCCCAAGAGGTATGCCAGATCAATTCACCTCCATTCTTTTAAACCAACTCCTTCGTGCTTTGGTTTATCTTCACGAAATGGGGTTTGTTCATAAAGCCATTAAAACAAGTAATATCTTCGTTGATTCAAAAGGCTGTGCTAAGCTCTCTGTTTTTGACATCATGCTCTCCAAGAACAATACTAATGAAATTGATCATGATTCTTCAATTCCTTATTGGGTTCCACCGGATGAAGAATATAGTTTCAAATCAGATATTTGGGGAATTGGGGTTTTAGCTATGGAAATTACTTGGGGGGGTCCTCCAATTATAGACCCAAGAATTCTTGAATCACAATTGTTTAATATCTCAAGAAGGTTTCCTTATGAATCGAAAACAGAGAAGATTGGTGACAGAAGATTCTTTGGAGACTCTGTTAAGGAATTTGTGAAATCTTGCCTTGTCAAAGATCGATTTCGAAGGCCTACGGCTTCGAGTTTGTTGACACATCCGTTTGTCAAGAAAGGGGAAAAACCGAATTTTTTGTTCAGGAGTATTCCTAAAGGAATGATTAACATTGATAAAAAATTTGAACAAGAAAAAGAAAGAATCATTGGGTTTATGAAAAATGACGAGAAATTGAAGAGTGAAAGTGACAAGACTATGAAGATTAGTGGATGGGATTATAATTTTTTTTTGTTTGAACTTGAACCTGATGGGAAATCAGTAGGGAAAAGAGTTCGATTTGGTGGGGAAGTCATAACGCTAGTTAAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGATGAAGAAGAAAAAGAAGAGAGCAAGAAAGTTGATGATATGTCATCTTTACTGCAAAAAATACAGAGTAATTTGAGTGATGCTGGTGAACCAAGCAAGGCTGGTGAGAAGAAGAAGAAGAAGAGCAAGAAGAATTTTGATGAAGAAAGTTTCGTGAAAATGTTGTCTTCATTCTTAGGTAATATTGATCAACAAAGGAAGATCTCTTTGATTTTGATAAATCATCTTGATCATTTGACTGAAAAACAGTTGAAAATAAGTAATGAAATCAATGAGTTACAGGCAGATTTGGCTTATGAGAAAGAAAGATACTTTCAATTAGAGTTGGAATGTGAATTTCTAAAGCTCAAAATTCCTGATTCTGAAGATGAAGATGAATATTATGATGAAGAACAGCAAGATTCTCAAGAGTGA

mRNA sequence

ATGAAGCACCATCAAGTTCAAGAAACCACCGCCACCTCCGCCGCTAGTACACTGAATTTCAACTTTCCGACGACCTCCGATGGCTACACAATCATTACTCAGATCGGTGCCAGCCCCCACAGCATTGTGTACATGGCCCATTGCAAGTCGGAAGACTCGACGACGATCACCGTCGCCGTAAAGATTATGGATATCAATATCAATCTCGACCTCAATTCCCTAAACGCTTCACTATCACTGATCAACCACCCCAATATTCTCCGGCCACATTGTTGTTTCGTCGGCGACGACGGCCGCATTTGGATCGTAATGCCCTTCATTCCAACCGGCCCTTTACAATCCGTTATTTCTCACAGTTTCCCAAGAGGTATGCCAGATCAATTCACCTCCATTCTTTTAAACCAACTCCTTCGTGCTTTGGTTTATCTTCACGAAATGGGGTTTGTTCATAAAGCCATTAAAACAAGTAATATCTTCGTTGATTCAAAAGGCTGTGCTAAGCTCTCTGTTTTTGACATCATGCTCTCCAAGAACAATACTAATGAAATTGATCATGATTCTTCAATTCCTTATTGGGTTCCACCGGATGAAGAATATAGTTTCAAATCAGATATTTGGGGAATTGGGGTTTTAGCTATGGAAATTACTTGGGGGGGTCCTCCAATTATAGACCCAAGAATTCTTGAATCACAATTGTTTAATATCTCAAGAAGGTTTCCTTATGAATCGAAAACAGAGAAGATTGGTGACAGAAGATTCTTTGGAGACTCTGTTAAGGAATTTGTGAAATCTTGCCTTGTCAAAGATCGATTTCGAAGGCCTACGGCTTCGAGTTTGTTGACACATCCGTTTGTCAAGAAAGGGGAAAAACCGAATTTTTTGTTCAGGAGTATTCCTAAAGGAATGATTAACATTGATAAAAAATTTGAACAAGAAAAAGAAAGAATCATTGGGTTTATGAAAAATGACGAGAAATTGAAGAGTGAAAGTGACAAGACTATGAAGATTAGTGGATGGGATTATAATTTTTTTTTGTTTGAACTTGAACCTGATGGGAAATCAGTAGGGAAAAGAGTTCGATTTGGTGGGGAAGTCATAACGCTAGTTAAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGATGAAGAAGAAAAAGAAGAGAGCAAGAAAGTTGATGATATGTCATCTTTACTGCAAAAAATACAGAGTAATTTGAGTGATGCTGGTGAACCAAGCAAGGCTGGTGAGAAGAAGAAGAAGAAGAGCAAGAAGAATTTTGATGAAGAAAGTTTCGTGAAAATGTTGTCTTCATTCTTAGGTAATATTGATCAACAAAGGAAGATCTCTTTGATTTTGATAAATCATCTTGATCATTTGACTGAAAAACAGTTGAAAATAAGTAATGAAATCAATGAGTTACAGGCAGATTTGGCTTATGAGAAAGAAAGATACTTTCAATTAGAGTTGGAATGTGAATTTCTAAAGCTCAAAATTCCTGATTCTGAAGATGAAGATGAATATTATGATGAAGAACAGCAAGATTCTCAAGAGTGA

Coding sequence (CDS)

ATGAAGCACCATCAAGTTCAAGAAACCACCGCCACCTCCGCCGCTAGTACACTGAATTTCAACTTTCCGACGACCTCCGATGGCTACACAATCATTACTCAGATCGGTGCCAGCCCCCACAGCATTGTGTACATGGCCCATTGCAAGTCGGAAGACTCGACGACGATCACCGTCGCCGTAAAGATTATGGATATCAATATCAATCTCGACCTCAATTCCCTAAACGCTTCACTATCACTGATCAACCACCCCAATATTCTCCGGCCACATTGTTGTTTCGTCGGCGACGACGGCCGCATTTGGATCGTAATGCCCTTCATTCCAACCGGCCCTTTACAATCCGTTATTTCTCACAGTTTCCCAAGAGGTATGCCAGATCAATTCACCTCCATTCTTTTAAACCAACTCCTTCGTGCTTTGGTTTATCTTCACGAAATGGGGTTTGTTCATAAAGCCATTAAAACAAGTAATATCTTCGTTGATTCAAAAGGCTGTGCTAAGCTCTCTGTTTTTGACATCATGCTCTCCAAGAACAATACTAATGAAATTGATCATGATTCTTCAATTCCTTATTGGGTTCCACCGGATGAAGAATATAGTTTCAAATCAGATATTTGGGGAATTGGGGTTTTAGCTATGGAAATTACTTGGGGGGGTCCTCCAATTATAGACCCAAGAATTCTTGAATCACAATTGTTTAATATCTCAAGAAGGTTTCCTTATGAATCGAAAACAGAGAAGATTGGTGACAGAAGATTCTTTGGAGACTCTGTTAAGGAATTTGTGAAATCTTGCCTTGTCAAAGATCGATTTCGAAGGCCTACGGCTTCGAGTTTGTTGACACATCCGTTTGTCAAGAAAGGGGAAAAACCGAATTTTTTGTTCAGGAGTATTCCTAAAGGAATGATTAACATTGATAAAAAATTTGAACAAGAAAAAGAAAGAATCATTGGGTTTATGAAAAATGACGAGAAATTGAAGAGTGAAAGTGACAAGACTATGAAGATTAGTGGATGGGATTATAATTTTTTTTTGTTTGAACTTGAACCTGATGGGAAATCAGTAGGGAAAAGAGTTCGATTTGGTGGGGAAGTCATAACGCTAGTTAAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGATGAAGAAGAAAAAGAAGAGAGCAAGAAAGTTGATGATATGTCATCTTTACTGCAAAAAATACAGAGTAATTTGAGTGATGCTGGTGAACCAAGCAAGGCTGGTGAGAAGAAGAAGAAGAAGAGCAAGAAGAATTTTGATGAAGAAAGTTTCGTGAAAATGTTGTCTTCATTCTTAGGTAATATTGATCAACAAAGGAAGATCTCTTTGATTTTGATAAATCATCTTGATCATTTGACTGAAAAACAGTTGAAAATAAGTAATGAAATCAATGAGTTACAGGCAGATTTGGCTTATGAGAAAGAAAGATACTTTCAATTAGAGTTGGAATGTGAATTTCTAAAGCTCAAAATTCCTGATTCTGAAGATGAAGATGAATATTATGATGAAGAACAGCAAGATTCTCAAGAGTGA

Protein sequence

MKHHQVQETTATSAASTLNFNFPTTSDGYTIITQIGASPHSIVYMAHCKSEDSTTITVAVKIMDININLDLNSLNASLSLINHPNILRPHCCFVGDDGRIWIVMPFIPTGPLQSVISHSFPRGMPDQFTSILLNQLLRALVYLHEMGFVHKAIKTSNIFVDSKGCAKLSVFDIMLSKNNTNEIDHDSSIPYWVPPDEEYSFKSDIWGIGVLAMEITWGGPPIIDPRILESQLFNISRRFPYESKTEKIGDRRFFGDSVKEFVKSCLVKDRFRRPTASSLLTHPFVKKGEKPNFLFRSIPKGMINIDKKFEQEKERIIGFMKNDEKLKSESDKTMKISGWDYNFFLFELEPDGKSVGKRVRFGGEVITLVKEEEEEEEEEEEDEEEKEESKKVDDMSSLLQKIQSNLSDAGEPSKAGEKKKKKSKKNFDEESFVKMLSSFLGNIDQQRKISLILINHLDHLTEKQLKISNEINELQADLAYEKERYFQLELECEFLKLKIPDSEDEDEYYDEEQQDSQE

Homology

[Close]

BLAST of Lsi09G012550 vs. ExPASy Swiss-Prot
Match: O23304 (Serine/threonine-protein kinase BLUS1 OS=Arabidopsis thaliana OX=3702 GN=BLUS1 PE=1 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 4.0e-45
Identity = 148/512 (28.91%), Postives = 255/512 (49.80%), Query Frame = 0


Query: 22  FPTTSDGYTIITQIGASPHSIVYMAHCKSEDSTTITVAVKIMDIN-INLDLNSL---NAS 81
           FP  ++ Y II +IG    + VY A C   +S  + VA+K +D++    D +SL     +
Sbjct: 8   FPLDAEAYEIICKIGVGVSASVYKAICIPMNS--MVVAIKAIDLDQSRADFDSLRRETKT 67

Query: 82  LSLINHPNILRPHCCFVGDDGRIWIVMPFIPTGPLQSVISHSFPRGMPDQFTSILLNQLL 141
           +SL++HPNIL  +C F   D  +W+VMPF+  G L S++S SFP G+P+   S+ L + L
Sbjct: 68  MSLLSHPNILNAYCSFT-VDRCLWVVMPFMSCGSLHSIVSSSFPSGLPENCISVFLKETL 127

Query: 142 RALVYLHEMGFVHKAIKTSNIFVDSKGCAKL-------SVFDIMLSKNNTN----EIDHD 201
            A+ YLH+ G +H+ IK  NI VDS G  KL       S+++ + S + T      +   
Sbjct: 128 NAISYLHDQGHLHRDIKAGNILVDSDGSVKLADFGVSASIYEPVTSSSGTTSSSLRLTDI 187

Query: 202 SSIPYWVPPD-----EEYSFKSDIWGIGVLAMEITWGGPPIIDPRILESQLFNISRRFPY 261
           +  PYW+ P+       Y FK+DIW  G+ A+E+  G PP+     L+S L  I++RF +
Sbjct: 188 AGTPYWMAPEVVHSHTGYGFKADIWSFGITALELAHGRPPLSHLPPLKSLLMKITKRFHF 247

Query: 262 E------SKTEKIGDRRFFGDSVKEFVKSCLVKDRFRRPTASSLLTHPFVKKGEKPNFLF 321
                  S + K G+++ F  + +E V  CL +D  +RP+A  LL HPF K  +  +F+ 
Sbjct: 248 SDYEINTSGSSKKGNKK-FSKAFREMVGLCLEQDPTKRPSAEKLLKHPFFKNCKGLDFVV 307

Query: 322 RSIPKGMINIDKKFEQEKERIIGFMKNDEKLKSESD----KTMKISGWDYNFFLFELEPD 381
           +++   + N ++ F  E + +I  + +D++ + E D    K  +ISGW++     +L P 
Sbjct: 308 KNVLHSLSNAEQMF-MESQILIKSVGDDDEEEEEEDEEIVKNRRISGWNFREDDLQLSP- 367

Query: 382 GKSVGKRVRFGGEVITLVKEEEEEEEEEEEDEEEKEESKKVDDMSSLLQKIQSNLSDAGE 441
                               E +  E    +E++ ++ K+ D+++    ++   LS+   
Sbjct: 368 ---------------VFPATESDSSESSPREEDQSKDKKEDDNVTITGYELGLGLSNEEA 427

Query: 442 PSKAGEKKKKKSKKNFDEESFVKMLSSFLGNIDQQRKISLILINHLDHLTEKQLKISNEI 501
            ++ GE         FD++  ++ L     +++ QR    I+I  L    E++ +   E+
Sbjct: 428 KNQEGE------VVGFDKDLVLEKLKVLKKSLEHQRARVSIIIEALSGDKEEKSR-EEEL 483

Query: 502 NELQADLAYEKERYFQLELECEFLKLKIPDSE 504
            E+   L        ++ELE E LK    D +
Sbjct: 488 LEMVEKL--------KIELETEKLKTLRADKD 483

[Close]

BLAST of Lsi09G012550 vs. ExPASy Swiss-Prot
Match: Q551H4 (Serine/threonine-protein kinase fray2 OS=Dictyostelium discoideum OX=44689 GN=fray2 PE=3 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 2.2e-35
Identity = 94/346 (27.17%), Postives = 174/346 (50.29%), Query Frame = 0


Query: 9   TTATSAASTL----NFNFPTTSDGYTIITQIGASPHSIVYMAHCKSEDSTTITVAVKIMD 68
           +T T+AAS +      N+P ++D Y +   IG     +V  A C         VA+KI+D
Sbjct: 47  STTTTAASPITPKPRKNYPNSADQYELKETIGKGGSGLVQRAICLPFQE---NVAIKIID 106

Query: 69  I----NINL-DLNSLNASLSLINHPNILRPHCCFVGDDGRIWIVMPFIPTGPLQSVISHS 128
           +    N++L ++     ++SL +HPN++  H  FV ++  +W++M F+  G    ++  S
Sbjct: 107 LEHCKNVSLEEIRKEIQAMSLCHHPNVVAYHTSFVYNES-LWVIMDFLSAGSCSDIMRFS 166

Query: 129 FPRGMPDQFTSILLNQLLRALVYLHEMGFVHKAIKTSNIFVDSKGCAKLSVFDIMLSKNN 188
           FP+G  +   + +L + L+A+ Y H+ G +H+ IK+ NI +DS G  +LS F +  +  +
Sbjct: 167 FPQGFEEHVIATILKEALKAICYFHKTGRIHRDIKSGNILIDSNGNIQLSDFGVSATLID 226

Query: 189 TNEIDHDSSI--PYWVPPD----EEYSFKSDIWGIGVLAMEITWGGPPIIDPRILESQLF 248
           T E   ++ +  P W+ P+      Y +  DIW  G+ A+E+  G  P  +   ++  L 
Sbjct: 227 TGETSRNTFVGTPCWMAPEIMEQVNYDYAVDIWSFGITALELARGKAPFAEYPPMKVLLL 286

Query: 249 NISRRFPYESKTEKIGDRRFFGDSVKEFVKSCLVKDRFRRPTASSLLTHPFVKKGEKPNF 308
            +    P     E  G+ + +  S K+ V+ CL KD  +RP  S LL H F K+ +KP++
Sbjct: 287 TLQNPPP---SLEGDGESK-WSHSFKDLVEKCLQKDPSKRPLPSKLLEHRFFKQAKKPDY 346

Query: 309 LFRSIPKGMINIDKKFEQEKERIIGFMKNDEKLKSESDKTMKISGW 340
           L + I   +  + ++++   E     ++N    + ++  +     W
Sbjct: 347 LVQHILAKLPPLGQRYQMLSEDSFAMLRNTSSPQFDTGHSNSADEW 384

[Close]

BLAST of Lsi09G012550 vs. ExPASy Swiss-Prot
Match: P97820 (Mitogen-activated protein kinase kinase kinase kinase 4 OS=Mus musculus OX=10090 GN=Map4k4 PE=1 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 3.1e-21
Identity = 101/429 (23.54%), Postives = 196/429 (45.69%), Query Frame = 0


Query: 29  YTIITQIGASPHSIVYMAHCKSEDSTTITVAVKIMDI------NINLDLNSLNASLSLIN 88
           + ++  +G   +  VY    K     T+T A+K+MD+       I L++N L       +
Sbjct: 25  FELVEVVGNGTYGQVY----KGRHVKTVTAAIKVMDVTEDEEEEITLEINMLK---KYSH 84

Query: 89  HPNILRPHCCFV-----GDDGRIWIVMPFIPTGPLQSVISHSFPRGMPDQFTSILLNQLL 148
           H NI   +  F+     G D ++W+VM F   G +  ++ ++    + + + + +  ++L
Sbjct: 85  HRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREIL 144

Query: 149 RALVYLHEMGFVHKAIKTSNIFVDSKGCAKLSVFDIMLSKNNT-NEIDHDSSIPYWVP-- 208
           R L +LH    +H+ IK  N+ +      KL  F +    + T    +     PYW+   
Sbjct: 145 RGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPE 204

Query: 209 -------PDEEYSFKSDIWGIGVLAMEITWGGPPIIDPRILESQLFNISRRFPYESKTEK 268
                  PD  Y ++SD+W  G+ A+E+  GGPP+ D   + + LF I R  P   K++K
Sbjct: 205 VIACDENPDATYDYRSDLWSCGITAIEMAEGGPPLCDMHPMRA-LFLIPRNPPPRLKSKK 264

Query: 269 IGDRRFFGDSVKEFVKSCLVKDRFRRPTASSLLTHPFVKKGEKPN-FLFRSIPKGMINID 328
              ++FF      F++ CLVK+  +RP+   LL HPF++  ++PN    R   K  I+  
Sbjct: 265 -WSKKFF-----SFIEGCLVKNYMQRPSTEQLLKHPFIR--DQPNERQVRIQLKDHIDRT 324

Query: 329 KKFEQEKE----RIIGFMKNDEKLKSESDKTMKI------SGWDYNFFLFELEPDGKSVG 388
           +K   EK+       G  + +E++  +  +   I      S    +F   + E   +S  
Sbjct: 325 RKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEA 384

Query: 389 KRVRFGGEVITLVKEEEEEEEEEEEDEEEKEESKKVDDMSSLLQKIQSNLSDAGEPSKAG 426
            R +       L++E++  E+EE + +   E  K+++      ++++       E  +  
Sbjct: 385 LRRQ------QLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQ 431

[Close]

BLAST of Lsi09G012550 vs. ExPASy Swiss-Prot
Match: Q9JI10 (Serine/threonine-protein kinase 3 OS=Mus musculus OX=10090 GN=Stk3 PE=1 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 1.2e-20
Identity = 98/384 (25.52%), Postives = 176/384 (45.83%), Query Frame = 0


Query: 29  YTIITQIGASPHSIVYMAHCKSEDSTTITVAVKIMDININLDLNSLNASLSLI---NHPN 88
           + ++ ++G   +  V+ A  K          V I  + +  DL  +   +S++   + P 
Sbjct: 27  FDVLEKLGEGSYGSVFKAIHKESGQ-----VVAIKQVPVESDLQEIIKEISIMQQCDSPY 86

Query: 89  ILRPHCCFVGDDGRIWIVMPFIPTGPLQSVISHSFPRGMPDQFTSILLNQLLRALVYLHE 148
           +++ +  +  +   +WIVM +   G +  +I         D+  +I L   L+ L YLH 
Sbjct: 87  VVKYYGSYFKNTD-LWIVMEYCGAGSVSDIIRLRNKTLTEDEIATI-LKSTLKGLEYLHF 146

Query: 149 MGFVHKAIKTSNIFVDSKGCAKLSVFDI------MLSKNNTNEIDHDSSIPYWVPPDE-- 208
           M  +H+ IK  NI ++++G AKL+ F +       ++K NT         P+W+ P+   
Sbjct: 147 MRKIHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAKRNT-----VIGTPFWMAPEVIQ 206

Query: 209 --EYSFKSDIWGIGVLAMEITWGGPPIIDPRILESQLFNISRRFPYESKTEKIGDRRFFG 268
              Y+  +DIW +G+ ++E+  G PP  D   + + +F I    P   +  ++     + 
Sbjct: 207 EIGYNCVADIWSLGITSIEMAEGKPPYADIHPMRA-IFMIPTNPPPTFRKPEL-----WS 266

Query: 269 DSVKEFVKSCLVKDRFRRPTASSLLTHPFVKKGEKPNFLFRSIPKGMINIDKKFEQEKER 328
           D   +FVK CLVK   +R TA+ LL HPF+K  +  + L   I + M    K+ E+++  
Sbjct: 267 DDFTDFVKKCLVKSPEQRATATQLLQHPFIKNAKPVSILRDLIAEAMEIKAKRHEEQQRE 326

Query: 329 IIGFMKNDE----------KLKSESDKTMKISGWDYNFFLFELEPDGKSVGKRVRFGGEV 388
           +    +N +          K  SES  TM+ +          +E +   +   +   G +
Sbjct: 327 LEEEEENSDEDELDSHTMVKTSSESVGTMRATSTMSEGAQTMIEHNSTMLESDL---GTM 386

Query: 389 ITLVKEEEEEEEEEEEDEEEKEES 390
           +   +EEEEEEEEEEED   K  +
Sbjct: 387 VINSEEEEEEEEEEEEDGTMKRNA 389

[Close]

BLAST of Lsi09G012550 vs. ExPASy Swiss-Prot
Match: P83510 (Traf2 and NCK-interacting protein kinase OS=Mus musculus OX=10090 GN=Tnik PE=1 SV=2)

HSP 1 Score: 100.9 bits (250), Expect = 4.4e-20
Identity = 114/486 (23.46%), Postives = 209/486 (43.00%), Query Frame = 0


Query: 29  YTIITQIGASPHSIVYMA-HCKSEDSTTITVAVKIMDI------NINLDLNSLNASLSLI 88
           + ++  +G   +  VY   H K    T    A+K+MD+       I  ++N L       
Sbjct: 25  FELVELVGNGTYGQVYKGRHVK----TGQLAAIKVMDVTGDEEEEIKQEINMLK---KYS 84

Query: 89  NHPNILRPHCCFV-----GDDGRIWIVMPFIPTGPLQSVISHSFPRGMPDQFTSILLNQL 148
           +H NI   +  F+     G D ++W+VM F   G +  +I ++    + +++ + +  ++
Sbjct: 85  HHRNIATYYGAFIKKNPPGMDDQLWLVMEFCGAGSVTDLIKNTKGNTLKEEWIAYICREI 144

Query: 149 LRALVYLHEMGFVHKAIKTSNIFVDSKGCAKLSVFDIMLSKNNT-NEIDHDSSIPYWVP- 208
           LR L +LH+   +H+ IK  N+ +      KL  F +    + T    +     PYW+  
Sbjct: 145 LRGLSHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAP 204

Query: 209 --------PDEEYSFKSDIWGIGVLAMEITWGGPPIIDPRILESQLFNISRRFPYESKTE 268
                   PD  Y FKSD+W +G+ A+E+  G PP+ D   + + LF I R      K++
Sbjct: 205 EVIACDENPDATYDFKSDLWSLGITAIEMAEGAPPLCDMHPMRA-LFLIPRNPAPRLKSK 264

Query: 269 KIGDRRFFGDSVKEFVKSCLVKDRFRRPTASSLLTHPFVKKGEKPN-FLFRSIPKGMINI 328
           K      +    + F++SCLVK+  +RP    L+ HPF++  ++PN    R   K  I+ 
Sbjct: 265 K------WSKKFQSFIESCLVKNHSQRPATEQLMKHPFIR--DQPNERQVRIQLKDHIDR 324

Query: 329 DKKFEQEKERIIGFMKNDEKLKSESDKTMKISGWDYNFFLFELEPDGKSVGKRVRFGGEV 388
            KK   EK+         E+ + E+D     S          L   G+S  +R     ++
Sbjct: 325 TKKKRGEKDETEYEYSGSEEEEEENDSGEPSS---------ILNLPGESTLRRDFLRLQL 384

Query: 389 ITLVKEEEEEEEEEEEDEEEKEESKKVDDMSSLLQKIQSNLSDAGEPSKAGEKKKKKSK- 448
               + E    ++ E+ + E EE K+      LL + Q  + +  E  +  E+++++ K 
Sbjct: 385 ANKERSEALRRQQLEQQQRENEEHKR-----QLLAERQKRIEEQKEQRRRLEEQQRREKE 444

Query: 449 -KNFDEESFVKMLSSFLGNIDQQRKISLILINHLDHLTEKQLKISNEINELQADLAYEKE 490
            +   E    +     +   +++R+       H      KQL+   +   LQ  L  E++
Sbjct: 445 LRKQQEREQRRHYEEQMRREEERRR-----AEHEQEYKRKQLEEQRQAERLQRQLKQERD 475

[Close]

BLAST of Lsi09G012550 vs. ExPASy TrEMBL
Match: A0A5D3C8A9 (Serine/threonine-protein kinase BLUS1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1503G00250 PE=4 SV=1)

HSP 1 Score: 633.6 bits (1633), Expect = 7.0e-178
Identity = 336/506 (66.40%), Postives = 402/506 (79.45%), Query Frame = 0


Query: 22  FPTTSDGYTIITQIGASPHSIVYMAHCKSED---STTITVAVKIMDI-NINLDLNSLNAS 81
           FPTTSD YTI+TQIGASP+S VY+A C S+D   S+T+TVA+KI++   I  D N LN S
Sbjct: 3   FPTTSDQYTILTQIGASPNSAVYLAKCDSQDSSTSSTVTVAIKIIETPQIIFDTNLLNTS 62

Query: 82  LSLINHPNILRPHCCFVGDDGRIWIVMPFIPTGPLQSVISHSFPRGMPDQFTSILLNQLL 141
           L+LI+HPNI+RPHCCF  DD R+WIVMP++  GPLQSV+SHSFPRGMPDQFTSILL+Q+L
Sbjct: 63  LTLIDHPNIVRPHCCFFADDNRLWIVMPYMSAGPLQSVLSHSFPRGMPDQFTSILLSQVL 122

Query: 142 RALVYLHEMGFVHKAIKTSNIFVDSKGCAKLSVFDIMLSKNNTNEIDHDSSIPYWVPPDE 201
           +ALVYLH MGFVHKAIK SNIF DS+G  KLSVFD+M+S +  N+ID DSSIPYWVPPD+
Sbjct: 123 QALVYLHRMGFVHKAIKPSNIFFDSQGTVKLSVFDLMISAD--NKIDRDSSIPYWVPPDD 182

Query: 202 EYSFKSDIWGIGVLAMEITWGGPPIIDPRILESQLFNISRRFPYESKTE----KIGDRRF 261
           EYSFKSDIW +GVLAME+TWGGPPIIDPRILESQ+ NISRRFPYE KTE      GDRR 
Sbjct: 183 EYSFKSDIWEVGVLAMEVTWGGPPIIDPRILESQVLNISRRFPYELKTETGRRNSGDRRI 242

Query: 262 FGDSVKEFVKSCLVKDRFRRPTASSLLTHPFVKKGEKP-NFLFRSIPKGMINIDKKFEQE 321
           FGD+VKEFVK+CL+KDR  RPTA+SLL HPFV KGE   NFLF+SIPKG+I ID++F+ E
Sbjct: 243 FGDAVKEFVKTCLLKDRLERPTAASLLDHPFVVKGENANNFLFKSIPKGVIEIDRRFQLE 302

Query: 322 KERIIGFMKNDEKLKSESDKTMKISGWDYNFFLFELEPDGKSVGKRVRFGGEVITLVKEE 381
           KERIIG M NDE LK++S+K  KISGW YN FLFELEPDG+S+GKRVRFGGE I      
Sbjct: 303 KERIIGIMNNDEGLKNQSEKITKISGWVYNDFLFELEPDGESLGKRVRFGGETI------ 362

Query: 382 EEEEEEEEEDEEEKEESKKVDDMSSLLQKIQSNLSDAGEPSKAGEKKKKKSKKNFDEESF 441
             E E+EEE+EEE E S KVDD  SLL+ I+ NL   G+ S    KKKKK KK  D+ES 
Sbjct: 363 -REFEKEEEEEEEGESSTKVDDTPSLLEIIEKNLMSGGDGSGESSKKKKKKKKIVDQESL 422

Query: 442 VKMLSSFLGNIDQQRKISLILINHLDHLTEKQLKISNEINELQADLAYEKERYFQLELEC 501
           VKML+S LGNID QR++  +LINH+ +L+EKQ ++SNEI  L+ +L  EKE+YFQLELE 
Sbjct: 423 VKMLTSVLGNIDAQREMVSMLINHVGYLSEKQSEMSNEIKRLETELKAEKEQYFQLELEY 482

Query: 502 EFLKLKIPDSEDEDEYYDEEQQDSQE 519
           EFLKLK+PDS+D+ + Y      S++
Sbjct: 483 EFLKLKVPDSDDDQKEYPSSSSSSEQ 499

[Close]

BLAST of Lsi09G012550 vs. ExPASy TrEMBL
Match: A0A1S3CN88 (serine/threonine-protein kinase BLUS1-like OS=Cucumis melo OX=3656 GN=LOC103502862 PE=4 SV=1)

HSP 1 Score: 633.6 bits (1633), Expect = 7.0e-178
Identity = 336/506 (66.40%), Postives = 402/506 (79.45%), Query Frame = 0


Query: 22  FPTTSDGYTIITQIGASPHSIVYMAHCKSED---STTITVAVKIMDI-NINLDLNSLNAS 81
           FPTTSD YTI+TQIGASP+S VY+A C S+D   S+T+TVA+KI++   I  D N LN S
Sbjct: 3   FPTTSDQYTILTQIGASPNSAVYLAKCDSQDSSTSSTVTVAIKIIETPQIIFDTNLLNTS 62

Query: 82  LSLINHPNILRPHCCFVGDDGRIWIVMPFIPTGPLQSVISHSFPRGMPDQFTSILLNQLL 141
           L+LI+HPNI+RPHCCF  DD R+WIVMP++  GPLQSV+SHSFPRGMPDQFTSILL+Q+L
Sbjct: 63  LTLIDHPNIVRPHCCFFADDNRLWIVMPYMSAGPLQSVLSHSFPRGMPDQFTSILLSQVL 122

Query: 142 RALVYLHEMGFVHKAIKTSNIFVDSKGCAKLSVFDIMLSKNNTNEIDHDSSIPYWVPPDE 201
           +ALVYLH MGFVHKAIK SNIF DS+G  KLSVFD+M+S +  N+ID DSSIPYWVPPD+
Sbjct: 123 QALVYLHRMGFVHKAIKPSNIFFDSQGTVKLSVFDLMISAD--NKIDRDSSIPYWVPPDD 182

Query: 202 EYSFKSDIWGIGVLAMEITWGGPPIIDPRILESQLFNISRRFPYESKTE----KIGDRRF 261
           EYSFKSDIW +GVLAME+TWGGPPIIDPRILESQ+ NISRRFPYE KTE      GDRR 
Sbjct: 183 EYSFKSDIWEVGVLAMEVTWGGPPIIDPRILESQVLNISRRFPYELKTETGRRNSGDRRI 242

Query: 262 FGDSVKEFVKSCLVKDRFRRPTASSLLTHPFVKKGEKP-NFLFRSIPKGMINIDKKFEQE 321
           FGD+VKEFVK+CL+KDR  RPTA+SLL HPFV KGE   NFLF+SIPKG+I ID++F+ E
Sbjct: 243 FGDAVKEFVKTCLLKDRLERPTAASLLDHPFVVKGENANNFLFKSIPKGVIEIDRRFQLE 302

Query: 322 KERIIGFMKNDEKLKSESDKTMKISGWDYNFFLFELEPDGKSVGKRVRFGGEVITLVKEE 381
           KERIIG M NDE LK++S+K  KISGW YN FLFELEPDG+S+GKRVRFGGE I      
Sbjct: 303 KERIIGIMNNDEGLKNQSEKITKISGWVYNDFLFELEPDGESLGKRVRFGGETI------ 362

Query: 382 EEEEEEEEEDEEEKEESKKVDDMSSLLQKIQSNLSDAGEPSKAGEKKKKKSKKNFDEESF 441
             E E+EEE+EEE E S KVDD  SLL+ I+ NL   G+ S    KKKKK KK  D+ES 
Sbjct: 363 -REFEKEEEEEEEGESSTKVDDTPSLLEIIEKNLMSGGDGSGESSKKKKKKKKIVDQESL 422

Query: 442 VKMLSSFLGNIDQQRKISLILINHLDHLTEKQLKISNEINELQADLAYEKERYFQLELEC 501
           VKML+S LGNID QR++  +LINH+ +L+EKQ ++SNEI  L+ +L  EKE+YFQLELE 
Sbjct: 423 VKMLTSVLGNIDAQREMVSMLINHVGYLSEKQSEMSNEIKRLETELKAEKEQYFQLELEY 482

Query: 502 EFLKLKIPDSEDEDEYYDEEQQDSQE 519
           EFLKLK+PDS+D+ + Y      S++
Sbjct: 483 EFLKLKVPDSDDDQKEYPSSSSSSEQ 499

[Close]

BLAST of Lsi09G012550 vs. ExPASy TrEMBL
Match: A0A6J1H6C5 (serine/threonine-protein kinase BLUS1-like OS=Cucurbita moschata OX=3662 GN=LOC111460018 PE=4 SV=1)

HSP 1 Score: 463.8 bits (1192), Expect = 9.6e-127
Identity = 261/529 (49.34%), Postives = 347/529 (65.60%), Query Frame = 0


Query: 1   MKHHQVQETTATSAASTLNFNFPTTSDGYTIITQIGASPHSIVYMAHCKSEDSTTITVAV 60
           M   Q+Q+   TSAA   +  FP  +D Y I +QIGAS HS VY A CKS D+ TI VA+
Sbjct: 1   MDRRQIQD-QETSAAKGTHSKFPKVADEYEIQSQIGASDHSTVYKAICKSADTPTI-VAI 60

Query: 61  KIMDININLD---LNSLNASLSLINHPNILRPHCCFVGDDGRIWIVMPFIPTGPLQSVIS 120
           KI+D N+ +D   + ++N +LS INHP+IL PHCCF   DGR+WIVMPFIP GPLQ++IS
Sbjct: 61  KIIDSNVEIDQTAITNINTNLSFINHPHILTPHCCFTAPDGRLWIVMPFIPAGPLQAIIS 120

Query: 121 HSFPRGMPDQFTSILLNQLLRALVYLHEMGFVHKAIKTSNIFVDSKGCAKLSVFDIMLSK 180
             F  G+PDQF SIL+NQLLRALV+LHE G VHKA+K SNIF D+ GC  LS  D+    
Sbjct: 121 QRFRTGLPDQFMSILINQLLRALVFLHEKGLVHKAVKASNIFFDTHGCVLLSDLDV---- 180

Query: 181 NNTNEIDHD-SSIPYWVPPDEEYSFKSDIWGIGVLAMEITWGGPPIIDPRILESQLFNIS 240
             + E+  D SS+PYW PP+E+YSFKSD+W +G+LAMEI +G PPI D +++++Q   IS
Sbjct: 181 --SKEMKKDCSSVPYWDPPEEDYSFKSDVWSVGILAMEIAYGCPPIYDQKVIKAQFSKIS 240

Query: 241 RRFPY----ESKTEKIGDRRFFGDSVKEFVKSCLVKDRFRRPTASSLLTHPFVKKGEKPN 300
           RRFPY    ++++ K    R FGD++K FV SCL KDRF R +A SLL HPFVKKGE  N
Sbjct: 241 RRFPYDLNLDNRSGKSVVPRKFGDTLKHFVGSCLAKDRFARASALSLLDHPFVKKGENSN 300

Query: 301 FLFRSIPKGMINIDKKFEQEKERIIGFMKNDEKLKSESD---KTMKISGWDYNFFLFELE 360
           FLF+SI KGM+ I + FEQ+K+RII  MKN+ +L   S    K ++I+GWDYN FLFELE
Sbjct: 301 FLFKSIAKGMVGIGEMFEQDKDRIIDLMKNEVQLGESSQGGGKVLRITGWDYNNFLFELE 360

Query: 361 PDGKSVGKRVRFGGEVITLVKEEEEEEEEEEEDEEEKEESKKVDDMSSLLQKIQSNLSDA 420
           PD +S GKRVRF GE +   KE+E+  +                DM SL+          
Sbjct: 361 PDEESFGKRVRFRGETVFSYKEQEQCNK---------------FDMKSLMH--------- 420

Query: 421 GEPSKAGEKKKKKSKKNFDEESFVKMLSSFLGNIDQQRKISLILINHLDHLTEKQLKISN 480
                + ++KKK+  +  D E+   +LSS L NID Q+++  +L+NH   + +K L++  
Sbjct: 421 ---GGSSDEKKKEVDQAMDTETLATLLSSLLDNIDTQKEMVSMLMNHCGFMHDKDLEMVK 480

Query: 481 EINELQADLAYEKERYFQLELECEFLKLKIPDSEDEDEYYDEEQQDSQE 519
           +I  L+ +LA EKE+ FQLEL  E LKLK+PDS++E E  + E +   E
Sbjct: 481 QIKRLEDELAAEKEKSFQLELNFELLKLKLPDSDEEQEQEEYEPEPEPE 494

[Close]

BLAST of Lsi09G012550 vs. ExPASy TrEMBL
Match: A0A6J1KYZ4 (serine/threonine-protein kinase BLUS1-like OS=Cucurbita maxima OX=3661 GN=LOC111498878 PE=4 SV=1)

HSP 1 Score: 458.4 bits (1178), Expect = 4.0e-125
Identity = 258/530 (48.68%), Postives = 342/530 (64.53%), Query Frame = 0


Query: 1   MKHHQVQETTATSAASTLNFNFPTTSDGYTIITQIGASPHSIVYMAHCKSEDSTTITVAV 60
           M   Q+Q+   TSAA   +F FP  +D Y I +QIGAS HS VY A CKS DST I VA+
Sbjct: 1   MDRRQIQD-QETSAAKGTHFKFPKVADEYEIQSQIGASDHSTVYKAICKSADSTMI-VAI 60

Query: 61  KIMDININLD---LNSLNASLSLINHPNILRPHCCFVGDDGRIWIVMPFIPTGPLQSVIS 120
           KI+D N+ +D   + ++N +LS INHP+IL PHCCF+ +DG +WIVMPFI  GPLQ++IS
Sbjct: 61  KIIDSNVEIDPTAITNINTNLSFINHPHILSPHCCFIANDGCLWIVMPFIHAGPLQAIIS 120

Query: 121 HSFPRGMPDQFTSILLNQLLRALVYLHEMGFVHKAIKTSNIFVDSKGCAKLSVFDIMLSK 180
             F  G+PDQF SIL+NQLLRALV+LHE G VHK++K SNIF D+ GC  LS  D+    
Sbjct: 121 QRFRTGLPDQFMSILINQLLRALVFLHEKGLVHKSVKASNIFFDTHGCVLLSDLDVSKDM 180

Query: 181 NNTNEIDHDSSIPYWVPPDEEYSFKSDIWGIGVLAMEITWGGPPIIDPRILESQLFNISR 240
           N        SS+PYWVPP+E+YS KSD+W +G+LAMEI +G PPI D +++++Q   ISR
Sbjct: 181 NK-----DCSSVPYWVPPEEDYSSKSDVWSVGILAMEIAYGCPPIYDQKVIKAQFSKISR 240

Query: 241 RFPY----ESKTEKIGDRRFFGDSVKEFVKSCLVKDRFRRPTASSLLTHPFVKKGEKPNF 300
           RFPY    ++++ K    R FGD++K FV SCL KD F R +A SLL HPFVKKGE  NF
Sbjct: 241 RFPYDLNLDTRSGKSVVPRKFGDTLKHFVGSCLAKDPFARVSALSLLDHPFVKKGENSNF 300

Query: 301 LFRSIPKGMINIDKKFEQEKERIIGFMKNDEKLKSESD-----KTMKISGWDYNFFLFEL 360
           LF+SI KGM+ I + FEQ+K+RII  MKN+ +L   S      K ++I+GWDYN FLFEL
Sbjct: 301 LFKSIAKGMVGIGEMFEQDKDRIIDLMKNEVQLGESSQGGGGGKVLRITGWDYNNFLFEL 360

Query: 361 EPDGKSVGKRVRFGGEVITLVKEEEEEEEEEEEDEEEKEESKKVDDMSSLLQKIQSNLSD 420
           EPD +S GKRVRF GE +   KE+E+  +                DM SL+         
Sbjct: 361 EPDEESFGKRVRFRGETVFSYKEQEQCNK---------------FDMKSLMH-------- 420

Query: 421 AGEPSKAGEKKKKKSKKNFDEESFVKMLSSFLGNIDQQRKISLILINHLDHLTEKQLKIS 480
                  G  +KK+  +  D E+   +LSS L NID Q+++  +L+NH   + +K L++ 
Sbjct: 421 ------GGSDEKKEVDQAMDSETLATLLSSLLDNIDTQKEMVSMLMNHCGFMHDKDLEMV 480

Query: 481 NEINELQADLAYEKERYFQLELECEFLKLKIPDSEDEDEYYDEEQQDSQE 519
            +I  L+ +LA EKE+ FQLEL  E LKLK+PDS++E E  + E +   E
Sbjct: 481 KQIKRLEDELAAEKEKSFQLELNFELLKLKLPDSDEEQEQEEYEPEPEPE 494

[Close]

BLAST of Lsi09G012550 vs. ExPASy TrEMBL
Match: A0A6J1CA92 (serine/threonine-protein kinase BLUS1-like OS=Momordica charantia OX=3673 GN=LOC111009741 PE=4 SV=1)

HSP 1 Score: 448.4 bits (1152), Expect = 4.2e-122
Identity = 246/511 (48.14%), Postives = 353/511 (69.08%), Query Frame = 0


Query: 5   QVQETTATSAASTLNFNFPTTSDGYTIITQIGASPHSIVYMAHCKSEDSTTITVAVKIMD 64
           Q Q+    +AA  L+F FPT +DGY I+ QIG S HS+VY A C+S +++   VAVK++D
Sbjct: 6   QDQDPENAAAAGRLHFQFPTAADGYKILYQIGVSDHSVVYKAICESANNSA-KVAVKVID 65

Query: 65  ININLD---LNSLNASLSLINHPNILRPHCCFVGDDGRIWIVMPFIPTGPLQSVISHSFP 124
           ++ ++D   +  +N SLSLI+H NIL PHCCF   D R+W VMPFI  GPLQ+++++ FP
Sbjct: 66  VDRHVDEFFVMKMN-SLSLIDHANILSPHCCFT-VDRRLWTVMPFIHAGPLQAILANRFP 125

Query: 125 RGMPDQFTSILLNQLLRALVYLHEMGFVHKAIKTSNIFVDSKGCAKLSVFDIMLSKNNTN 184
            G+PDQF +ILL+QLLR+L+ LHE+G+VH+ IK SNIFVDS+G  KLS  ++ +  ++  
Sbjct: 126 NGLPDQFLAILLHQLLRSLICLHELGYVHRNIKASNIFVDSEGSVKLSDLNVYVGDDD-- 185

Query: 185 EIDHDSSIPYWVPPDEEYSFKSDIWGIGVLAMEITWGGPPIIDPRILESQLFNISRRFPY 244
             D DSS+PYW PPDEEYSFKSD+W IG++A+E+ +G PPI D R + SQL NISRRFPY
Sbjct: 186 --DDDSSVPYWDPPDEEYSFKSDVWAIGIVALEVAYGCPPISDSRTIRSQLLNISRRFPY 245

Query: 245 ESKTEKIGDR----RFFGDSVKEFVKSCLVKDRFRRPTASSLLTHPFVKKGEKPNFLFRS 304
               +K+  +    R FG+  K+FV SCLVKD  +RP+A+SLL HPF+KKGE  N LF+S
Sbjct: 246 NLNLDKMSSKSVIDRKFGELFKDFVSSCLVKDPSKRPSAASLLAHPFIKKGETSNVLFKS 305

Query: 305 IPKGMINIDKKFEQEKERIIGFMKNDEKLKSES-DKTMKISGWDYNFFLFELEPDGKSVG 364
           I K M  I+ +FE+ K+R++  MK D +++  +    +K+SGWD+N FLFELEPD +S+G
Sbjct: 306 IAKDMAGIEDRFEKNKDRLVELMKKDRRVEDNNRGNVVKVSGWDFNNFLFELEPDEESLG 365

Query: 365 KRVRFGGEVITLVKEEEEEEEEEEEDEEEKEESKKVDDMSSLLQKIQSNLSDAGEPSKAG 424
           KRVRF GE I  ++++     E  E  +             LL K   +LS +G   + G
Sbjct: 366 KRVRFRGETIIPLRDDHVSLNEAGEASKFN---------VPLLLKRMDSLSLSGLSGRMG 425

Query: 425 EKKKKKSKKNFDEESFVKMLSSFLGNIDQQRKISLILINHLDHLTEKQLKISNEINELQA 484
            + +++ +   D E+  K+L+S + +IDQQR++ +++I+    L E+ ++++ EI +L+A
Sbjct: 426 SRAEEEVRV-MDSETLAKVLTSLVESIDQQREVIMMVISQFGRLLERDMEMTKEIKKLKA 485

Query: 485 DLAYEKERYFQLELECEFLKLKIPDSEDEDE 508
           +LAYEKER FQLEL+ E LKLK+PDSEDED+
Sbjct: 486 ELAYEKERSFQLELDYEVLKLKLPDSEDEDK 499

[Close]

BLAST of Lsi09G012550 vs. NCBI nr
Match: XP_038875030.1 (serine/threonine-protein kinase BLUS1-like [Benincasa hispida])

HSP 1 Score: 722.2 bits (1863), Expect = 3.1e-204
Identity = 381/519 (73.41%), Postives = 416/519 (80.15%), Query Frame = 0


Query: 1   MKHHQVQETTATSAASTLNFNFPTTSDGYTIITQIGASPHSIVYMAHCKSEDSTTITVAV 60
           MK HQVQE+  T+A STL+F FPTTS+GY IITQIG SPHSIVYMA+C SE     TVAV
Sbjct: 1   MKQHQVQESATTTANSTLSFKFPTTSNGYKIITQIGVSPHSIVYMANCNSE-----TVAV 60

Query: 61  KIMDININLDLNSLNASLSLINHPNILRPHCCFVGDDGRIWIVMPFIPTGPLQSVISHSF 120
           KI+DININL+L+SLN SLSLI+HPNILRPHCCF+G+DGRIWIVMPFIPTGPLQS++S  F
Sbjct: 61  KIIDININLNLDSLNTSLSLIDHPNILRPHCCFIGEDGRIWIVMPFIPTGPLQSILSQIF 120

Query: 121 PRGMPDQFTSILLNQLLRALVYLHEMGFVHKAIKTSNIFVDSKGCAKLSVFDIMLSKNNT 180
           PRGMPD F SILLNQLLRALVYLHE+GFVHKAIK SNIF DS+G  KLSVFDIM+SKN+T
Sbjct: 121 PRGMPDHFISILLNQLLRALVYLHELGFVHKAIKPSNIFFDSQGSVKLSVFDIMISKNST 180

Query: 181 NEIDHDSSIPYWVPPDEEYSFKSDIWGIGVLAMEITWGGPPIIDPRILESQLFNISRRFP 240
           NEIDHDSSIPYWVPPDEEYSFKSD W +GVLAMEITWGGPPIIDPRILESQ FNISRRFP
Sbjct: 181 NEIDHDSSIPYWVPPDEEYSFKSDAWAVGVLAMEITWGGPPIIDPRILESQFFNISRRFP 240

Query: 241 YESKTEKIGDRRFFGDSVKEFVKSCLVKDRFRRPTASSLLTHPFVKKGEKPNFLFRSIPK 300
           YE K E+  DRR FGDSVKEFVK CLVKD+F RPTA SLL HPFVKKGE  NFLF+ IPK
Sbjct: 241 YEPKAERSRDRRLFGDSVKEFVKFCLVKDQFGRPTALSLLEHPFVKKGENSNFLFKCIPK 300

Query: 301 GMINIDKKFEQEKERIIGFMKNDEKL--KSESDKTMKISGWDYNFFLFELEPDGKSVGKR 360
           G+I IDK FEQEKERIIG MKNDEKL  KSE DK MKISGWDYN FLFELEPD +SVGKR
Sbjct: 301 GVIEIDKIFEQEKERIIGLMKNDEKLKNKSEGDKIMKISGWDYNNFLFELEPDEESVGKR 360

Query: 361 VRFGGEVITLVKEEEEEEEEEEEDEEEKEESKKVDDMSSLLQKIQSNLSDAGEPS-KAGE 420
           VRFGGEV                                      SNLS  GE S KAGE
Sbjct: 361 VRFGGEV--------------------------------------SNLSGVGESSKKAGE 420

Query: 421 --KKKKKSKKNFDEESFVKMLSSFLGNIDQQRKISLILINHLDHLTEKQLKISNEINELQ 480
             KKKKK KKN DE S VK L S L NID+QR++ L+LINH+ +LTEKQL++SNEI +L+
Sbjct: 421 KKKKKKKKKKNVDEGSLVKKLCSLLDNIDEQREMVLVLINHIGYLTEKQLEMSNEIKKLE 476

Query: 481 ADLAYEKERYFQLELECEFLKLKIPDSEDEDEYYDEEQQ 515
           ADLA EKERYFQLELECEFLKLK+PDSEDE+EY DEEQQ
Sbjct: 481 ADLASEKERYFQLELECEFLKLKVPDSEDENEYSDEEQQ 476

[Close]

BLAST of Lsi09G012550 vs. NCBI nr
Match: XP_008465200.1 (PREDICTED: serine/threonine-protein kinase BLUS1-like [Cucumis melo] >KAA0057526.1 serine/threonine-protein kinase BLUS1-like [Cucumis melo var. makuwa] >TYK08207.1 serine/threonine-protein kinase BLUS1-like [Cucumis melo var. makuwa])

HSP 1 Score: 633.6 bits (1633), Expect = 1.5e-177
Identity = 336/506 (66.40%), Postives = 402/506 (79.45%), Query Frame = 0


Query: 22  FPTTSDGYTIITQIGASPHSIVYMAHCKSED---STTITVAVKIMDI-NINLDLNSLNAS 81
           FPTTSD YTI+TQIGASP+S VY+A C S+D   S+T+TVA+KI++   I  D N LN S
Sbjct: 3   FPTTSDQYTILTQIGASPNSAVYLAKCDSQDSSTSSTVTVAIKIIETPQIIFDTNLLNTS 62

Query: 82  LSLINHPNILRPHCCFVGDDGRIWIVMPFIPTGPLQSVISHSFPRGMPDQFTSILLNQLL 141
           L+LI+HPNI+RPHCCF  DD R+WIVMP++  GPLQSV+SHSFPRGMPDQFTSILL+Q+L
Sbjct: 63  LTLIDHPNIVRPHCCFFADDNRLWIVMPYMSAGPLQSVLSHSFPRGMPDQFTSILLSQVL 122

Query: 142 RALVYLHEMGFVHKAIKTSNIFVDSKGCAKLSVFDIMLSKNNTNEIDHDSSIPYWVPPDE 201
           +ALVYLH MGFVHKAIK SNIF DS+G  KLSVFD+M+S +  N+ID DSSIPYWVPPD+
Sbjct: 123 QALVYLHRMGFVHKAIKPSNIFFDSQGTVKLSVFDLMISAD--NKIDRDSSIPYWVPPDD 182

Query: 202 EYSFKSDIWGIGVLAMEITWGGPPIIDPRILESQLFNISRRFPYESKTE----KIGDRRF 261
           EYSFKSDIW +GVLAME+TWGGPPIIDPRILESQ+ NISRRFPYE KTE      GDRR 
Sbjct: 183 EYSFKSDIWEVGVLAMEVTWGGPPIIDPRILESQVLNISRRFPYELKTETGRRNSGDRRI 242

Query: 262 FGDSVKEFVKSCLVKDRFRRPTASSLLTHPFVKKGEKP-NFLFRSIPKGMINIDKKFEQE 321
           FGD+VKEFVK+CL+KDR  RPTA+SLL HPFV KGE   NFLF+SIPKG+I ID++F+ E
Sbjct: 243 FGDAVKEFVKTCLLKDRLERPTAASLLDHPFVVKGENANNFLFKSIPKGVIEIDRRFQLE 302

Query: 322 KERIIGFMKNDEKLKSESDKTMKISGWDYNFFLFELEPDGKSVGKRVRFGGEVITLVKEE 381
           KERIIG M NDE LK++S+K  KISGW YN FLFELEPDG+S+GKRVRFGGE I      
Sbjct: 303 KERIIGIMNNDEGLKNQSEKITKISGWVYNDFLFELEPDGESLGKRVRFGGETI------ 362

Query: 382 EEEEEEEEEDEEEKEESKKVDDMSSLLQKIQSNLSDAGEPSKAGEKKKKKSKKNFDEESF 441
             E E+EEE+EEE E S KVDD  SLL+ I+ NL   G+ S    KKKKK KK  D+ES 
Sbjct: 363 -REFEKEEEEEEEGESSTKVDDTPSLLEIIEKNLMSGGDGSGESSKKKKKKKKIVDQESL 422

Query: 442 VKMLSSFLGNIDQQRKISLILINHLDHLTEKQLKISNEINELQADLAYEKERYFQLELEC 501
           VKML+S LGNID QR++  +LINH+ +L+EKQ ++SNEI  L+ +L  EKE+YFQLELE 
Sbjct: 423 VKMLTSVLGNIDAQREMVSMLINHVGYLSEKQSEMSNEIKRLETELKAEKEQYFQLELEY 482

Query: 502 EFLKLKIPDSEDEDEYYDEEQQDSQE 519
           EFLKLK+PDS+D+ + Y      S++
Sbjct: 483 EFLKLKVPDSDDDQKEYPSSSSSSEQ 499

[Close]

BLAST of Lsi09G012550 vs. NCBI nr
Match: XP_022958864.1 (serine/threonine-protein kinase BLUS1-like [Cucurbita moschata])

HSP 1 Score: 463.8 bits (1192), Expect = 2.0e-126
Identity = 261/529 (49.34%), Postives = 347/529 (65.60%), Query Frame = 0


Query: 1   MKHHQVQETTATSAASTLNFNFPTTSDGYTIITQIGASPHSIVYMAHCKSEDSTTITVAV 60
           M   Q+Q+   TSAA   +  FP  +D Y I +QIGAS HS VY A CKS D+ TI VA+
Sbjct: 1   MDRRQIQD-QETSAAKGTHSKFPKVADEYEIQSQIGASDHSTVYKAICKSADTPTI-VAI 60

Query: 61  KIMDININLD---LNSLNASLSLINHPNILRPHCCFVGDDGRIWIVMPFIPTGPLQSVIS 120
           KI+D N+ +D   + ++N +LS INHP+IL PHCCF   DGR+WIVMPFIP GPLQ++IS
Sbjct: 61  KIIDSNVEIDQTAITNINTNLSFINHPHILTPHCCFTAPDGRLWIVMPFIPAGPLQAIIS 120

Query: 121 HSFPRGMPDQFTSILLNQLLRALVYLHEMGFVHKAIKTSNIFVDSKGCAKLSVFDIMLSK 180
             F  G+PDQF SIL+NQLLRALV+LHE G VHKA+K SNIF D+ GC  LS  D+    
Sbjct: 121 QRFRTGLPDQFMSILINQLLRALVFLHEKGLVHKAVKASNIFFDTHGCVLLSDLDV---- 180

Query: 181 NNTNEIDHD-SSIPYWVPPDEEYSFKSDIWGIGVLAMEITWGGPPIIDPRILESQLFNIS 240
             + E+  D SS+PYW PP+E+YSFKSD+W +G+LAMEI +G PPI D +++++Q   IS
Sbjct: 181 --SKEMKKDCSSVPYWDPPEEDYSFKSDVWSVGILAMEIAYGCPPIYDQKVIKAQFSKIS 240

Query: 241 RRFPY----ESKTEKIGDRRFFGDSVKEFVKSCLVKDRFRRPTASSLLTHPFVKKGEKPN 300
           RRFPY    ++++ K    R FGD++K FV SCL KDRF R +A SLL HPFVKKGE  N
Sbjct: 241 RRFPYDLNLDNRSGKSVVPRKFGDTLKHFVGSCLAKDRFARASALSLLDHPFVKKGENSN 300

Query: 301 FLFRSIPKGMINIDKKFEQEKERIIGFMKNDEKLKSESD---KTMKISGWDYNFFLFELE 360
           FLF+SI KGM+ I + FEQ+K+RII  MKN+ +L   S    K ++I+GWDYN FLFELE
Sbjct: 301 FLFKSIAKGMVGIGEMFEQDKDRIIDLMKNEVQLGESSQGGGKVLRITGWDYNNFLFELE 360

Query: 361 PDGKSVGKRVRFGGEVITLVKEEEEEEEEEEEDEEEKEESKKVDDMSSLLQKIQSNLSDA 420
           PD +S GKRVRF GE +   KE+E+  +                DM SL+          
Sbjct: 361 PDEESFGKRVRFRGETVFSYKEQEQCNK---------------FDMKSLMH--------- 420

Query: 421 GEPSKAGEKKKKKSKKNFDEESFVKMLSSFLGNIDQQRKISLILINHLDHLTEKQLKISN 480
                + ++KKK+  +  D E+   +LSS L NID Q+++  +L+NH   + +K L++  
Sbjct: 421 ---GGSSDEKKKEVDQAMDTETLATLLSSLLDNIDTQKEMVSMLMNHCGFMHDKDLEMVK 480

Query: 481 EINELQADLAYEKERYFQLELECEFLKLKIPDSEDEDEYYDEEQQDSQE 519
           +I  L+ +LA EKE+ FQLEL  E LKLK+PDS++E E  + E +   E
Sbjct: 481 QIKRLEDELAAEKEKSFQLELNFELLKLKLPDSDEEQEQEEYEPEPEPE 494

[Close]

BLAST of Lsi09G012550 vs. NCBI nr
Match: KAG6574930.1 (Serine/threonine-protein kinase BLUS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 463.8 bits (1192), Expect = 2.0e-126
Identity = 261/529 (49.34%), Postives = 347/529 (65.60%), Query Frame = 0


Query: 1   MKHHQVQETTATSAASTLNFNFPTTSDGYTIITQIGASPHSIVYMAHCKSEDSTTITVAV 60
           M   Q+Q+   TSAA   +  FP  +D Y I +QIGAS HS VY A CKS D+ TI VA+
Sbjct: 1   MDRRQIQD-QETSAAKGTHSKFPKVADEYEIQSQIGASDHSTVYKAICKSADTPTI-VAI 60

Query: 61  KIMDININLD---LNSLNASLSLINHPNILRPHCCFVGDDGRIWIVMPFIPTGPLQSVIS 120
           KI+D N+ +D   + ++N +LS INHP+IL PHCCF   DGR+WIVMPFIP GPLQ++IS
Sbjct: 61  KIIDSNVEIDQTAITNINTNLSFINHPHILTPHCCFTAPDGRLWIVMPFIPAGPLQAIIS 120

Query: 121 HSFPRGMPDQFTSILLNQLLRALVYLHEMGFVHKAIKTSNIFVDSKGCAKLSVFDIMLSK 180
             F  G+PDQF SIL+NQLLRALV+LHE G VHKA+K SNIF D+ GC  LS  D+    
Sbjct: 121 QRFRTGLPDQFMSILINQLLRALVFLHEKGLVHKAVKASNIFFDTHGCVLLSDLDV---- 180

Query: 181 NNTNEIDHD-SSIPYWVPPDEEYSFKSDIWGIGVLAMEITWGGPPIIDPRILESQLFNIS 240
             + E+  D SS+PYW PP+E+YSFKSD+W +G+LAMEI +G PPI D +++++Q   IS
Sbjct: 181 --SKEMKKDCSSVPYWDPPEEDYSFKSDVWSVGILAMEIAYGCPPIYDQKVIKAQFSKIS 240

Query: 241 RRFPY----ESKTEKIGDRRFFGDSVKEFVKSCLVKDRFRRPTASSLLTHPFVKKGEKPN 300
           RRFPY    ++++ K    R FGD++K FV SCL KDRF R +A SLL HPFVKKGE  N
Sbjct: 241 RRFPYDLNLDNRSGKSVVPRKFGDTLKHFVGSCLAKDRFARASALSLLDHPFVKKGENSN 300

Query: 301 FLFRSIPKGMINIDKKFEQEKERIIGFMKNDEKLKSESD---KTMKISGWDYNFFLFELE 360
           FLF+SI KGM+ I + FEQ+K+RII  MKN+ +L   S    K ++I+GWDYN FLFELE
Sbjct: 301 FLFKSIAKGMVGIGEMFEQDKDRIIDLMKNEVQLGESSQGGGKVLRITGWDYNNFLFELE 360

Query: 361 PDGKSVGKRVRFGGEVITLVKEEEEEEEEEEEDEEEKEESKKVDDMSSLLQKIQSNLSDA 420
           PD +S GKRVRF GE +   KE+E+  +                DM SL+          
Sbjct: 361 PDEESFGKRVRFRGETVFSYKEQEQCNK---------------FDMKSLMH--------- 420

Query: 421 GEPSKAGEKKKKKSKKNFDEESFVKMLSSFLGNIDQQRKISLILINHLDHLTEKQLKISN 480
                + ++KKK+  +  D E+   +LSS L NID Q+++  +L+NH   + +K L++  
Sbjct: 421 ---GGSSDEKKKEVDQAMDTETLATLLSSLLDNIDTQKEMVSMLMNHCGFMHDKDLEMVK 480

Query: 481 EINELQADLAYEKERYFQLELECEFLKLKIPDSEDEDEYYDEEQQDSQE 519
           +I  L+ +LA EKE+ FQLEL  E LKLK+PDS++E E  + E +   E
Sbjct: 481 QIKRLEDELAAEKEKSFQLELNFELLKLKLPDSDEEQEQEEYEPEPEPE 494

[Close]

BLAST of Lsi09G012550 vs. NCBI nr
Match: KAG7013499.1 (Serine/threonine-protein kinase BLUS1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 463.8 bits (1192), Expect = 2.0e-126
Identity = 262/528 (49.62%), Postives = 348/528 (65.91%), Query Frame = 0


Query: 5   QVQETTATSAAST---LNFNFPTTSDGYTIITQIGASPHSIVYMAHCKSEDSTTITVAVK 64
           Q QET+A S+AS     +  FP  +D Y I +QIGAS HS VY A CKS D+ TI VA+K
Sbjct: 7   QDQETSAASSASAAKGTHSKFPKVADEYEIQSQIGASDHSTVYKAICKSADTPTI-VAIK 66

Query: 65  IMDININLD---LNSLNASLSLINHPNILRPHCCFVGDDGRIWIVMPFIPTGPLQSVISH 124
           I+D N+ +D   + ++N +LS INHP+IL PHCCF   DGR+WIVMPFIP GPLQ++IS 
Sbjct: 67  IIDSNVEIDQTAITNINTNLSFINHPHILTPHCCFTAPDGRLWIVMPFIPAGPLQAIISQ 126

Query: 125 SFPRGMPDQFTSILLNQLLRALVYLHEMGFVHKAIKTSNIFVDSKGCAKLSVFDIMLSKN 184
            F  G+PDQF SIL+NQLLRALV+LHE G VHKA+K SNIF D+ GC  LS  D+     
Sbjct: 127 RFRTGLPDQFMSILINQLLRALVFLHEKGLVHKAVKASNIFFDTHGCVLLSDLDV----- 186

Query: 185 NTNEIDHD-SSIPYWVPPDEEYSFKSDIWGIGVLAMEITWGGPPIIDPRILESQLFNISR 244
            + E+  D SS+PYW PP+E+YSFKSD+W +G+LAMEI +G PPI D +++++Q   ISR
Sbjct: 187 -SKEMKKDCSSVPYWDPPEEDYSFKSDVWSVGILAMEIAYGCPPIYDQKVIKAQFSKISR 246

Query: 245 RFPY----ESKTEKIGDRRFFGDSVKEFVKSCLVKDRFRRPTASSLLTHPFVKKGEKPNF 304
           RFPY    ++++ K    R FGD++K FV SCL KDRF R +A SLL HPFVKKGE  NF
Sbjct: 247 RFPYDLNLDNRSGKSVVPRKFGDTLKHFVGSCLAKDRFARASALSLLDHPFVKKGENSNF 306

Query: 305 LFRSIPKGMINIDKKFEQEKERIIGFMKNDEKLKSESD---KTMKISGWDYNFFLFELEP 364
           LF+SI KGM+ I + FEQ+K+RII  MKN+ +L   S    K ++I+GWDYN FLFELEP
Sbjct: 307 LFKSIAKGMVGIGEMFEQDKDRIIDLMKNEVQLGESSQGGGKVLRITGWDYNNFLFELEP 366

Query: 365 DGKSVGKRVRFGGEVITLVKEEEEEEEEEEEDEEEKEESKKVDDMSSLLQKIQSNLSDAG 424
           D +S GKRVRF GE +   KE+E+  +                DM SL+           
Sbjct: 367 DEESFGKRVRFRGETVFSYKEQEQCNK---------------FDMKSLM----------- 426

Query: 425 EPSKAGEKKKKKSKKNFDEESFVKMLSSFLGNIDQQRKISLILINHLDHLTEKQLKISNE 484
               + ++KKK+  +  D E+   +LSS L NID Q+++  +L+NH   + +K L++  +
Sbjct: 427 HGGGSSDEKKKEVDQAMDTETLATLLSSLLDNIDTQKEMVSMLMNHCGFMHDKDLEMVKQ 486

Query: 485 INELQADLAYEKERYFQLELECEFLKLKIPDSEDEDEYYDEEQQDSQE 519
           I  L+ +LA EKE+ FQLEL  E LKLK+PDS++E E  + E +   E
Sbjct: 487 IKRLEDELAAEKEKSFQLELNFELLKLKLPDSDEEQEQEEYEPEPEPE 501

[Close]

BLAST of Lsi09G012550 vs. TAIR 10
Match: AT4G14480.1 (Protein kinase superfamily protein )

HSP 1 Score: 184.1 bits (466), Expect = 2.8e-46
Identity = 148/512 (28.91%), Postives = 255/512 (49.80%), Query Frame = 0


Query: 22  FPTTSDGYTIITQIGASPHSIVYMAHCKSEDSTTITVAVKIMDIN-INLDLNSL---NAS 81
           FP  ++ Y II +IG    + VY A C   +S  + VA+K +D++    D +SL     +
Sbjct: 8   FPLDAEAYEIICKIGVGVSASVYKAICIPMNS--MVVAIKAIDLDQSRADFDSLRRETKT 67

Query: 82  LSLINHPNILRPHCCFVGDDGRIWIVMPFIPTGPLQSVISHSFPRGMPDQFTSILLNQLL 141
           +SL++HPNIL  +C F   D  +W+VMPF+  G L S++S SFP G+P+   S+ L + L
Sbjct: 68  MSLLSHPNILNAYCSFT-VDRCLWVVMPFMSCGSLHSIVSSSFPSGLPENCISVFLKETL 127

Query: 142 RALVYLHEMGFVHKAIKTSNIFVDSKGCAKL-------SVFDIMLSKNNTN----EIDHD 201
            A+ YLH+ G +H+ IK  NI VDS G  KL       S+++ + S + T      +   
Sbjct: 128 NAISYLHDQGHLHRDIKAGNILVDSDGSVKLADFGVSASIYEPVTSSSGTTSSSLRLTDI 187

Query: 202 SSIPYWVPPD-----EEYSFKSDIWGIGVLAMEITWGGPPIIDPRILESQLFNISRRFPY 261
           +  PYW+ P+       Y FK+DIW  G+ A+E+  G PP+     L+S L  I++RF +
Sbjct: 188 AGTPYWMAPEVVHSHTGYGFKADIWSFGITALELAHGRPPLSHLPPLKSLLMKITKRFHF 247

Query: 262 E------SKTEKIGDRRFFGDSVKEFVKSCLVKDRFRRPTASSLLTHPFVKKGEKPNFLF 321
                  S + K G+++ F  + +E V  CL +D  +RP+A  LL HPF K  +  +F+ 
Sbjct: 248 SDYEINTSGSSKKGNKK-FSKAFREMVGLCLEQDPTKRPSAEKLLKHPFFKNCKGLDFVV 307

Query: 322 RSIPKGMINIDKKFEQEKERIIGFMKNDEKLKSESD----KTMKISGWDYNFFLFELEPD 381
           +++   + N ++ F  E + +I  + +D++ + E D    K  +ISGW++     +L P 
Sbjct: 308 KNVLHSLSNAEQMF-MESQILIKSVGDDDEEEEEEDEEIVKNRRISGWNFREDDLQLSP- 367

Query: 382 GKSVGKRVRFGGEVITLVKEEEEEEEEEEEDEEEKEESKKVDDMSSLLQKIQSNLSDAGE 441
                               E +  E    +E++ ++ K+ D+++    ++   LS+   
Sbjct: 368 ---------------VFPATESDSSESSPREEDQSKDKKEDDNVTITGYELGLGLSNEEA 427

Query: 442 PSKAGEKKKKKSKKNFDEESFVKMLSSFLGNIDQQRKISLILINHLDHLTEKQLKISNEI 501
            ++ GE         FD++  ++ L     +++ QR    I+I  L    E++ +   E+
Sbjct: 428 KNQEGE------VVGFDKDLVLEKLKVLKKSLEHQRARVSIIIEALSGDKEEKSR-EEEL 483

Query: 502 NELQADLAYEKERYFQLELECEFLKLKIPDSE 504
            E+   L        ++ELE E LK    D +
Sbjct: 488 LEMVEKL--------KIELETEKLKTLRADKD 483

[Close]

BLAST of Lsi09G012550 vs. TAIR 10
Match: AT5G14720.1 (Protein kinase superfamily protein )

HSP 1 Score: 169.5 bits (428), Expect = 7.2e-42
Identity = 105/341 (30.79%), Postives = 170/341 (49.85%), Query Frame = 0


Query: 22  FPTTSDGYTIITQIGASPHSIVYMAHCKSEDSTTITVAVKIMDI-NINLDLNSLN---AS 81
           FP  +  Y +  +IG    + V+ A C       + VA+K++D+   N DL+ +     +
Sbjct: 9   FPLNAKDYKLYEEIGDGVSATVHRALC---IPLNVVVAIKVLDLEKCNNDLDGIRREVQT 68

Query: 82  LSLINHPNILRPHCCFVGDDGRIWIVMPFIPTGPLQSVISHSFPRGMPDQFTSILLNQLL 141
           +SLINHPN+L+ HC F     ++W+VMP++  G    +I  S+P G  +   + LL + L
Sbjct: 69  MSLINHPNVLQAHCSFT-TGHQLWVVMPYMAGGSCLHIIKSSYPDGFEEPVIATLLRETL 128

Query: 142 RALVYLHEMGFVHKAIKTSNIFVDSKGCAKLSVFDIMLSKNNTNEIDHDSS----IPYWV 201
           +ALVYLH  G +H+ +K  NI +DS G  KL+ F +     +T +     +     P W+
Sbjct: 129 KALVYLHAHGHIHRDVKAGNILLDSNGAVKLADFGVSACMFDTGDRQRSRNTFVGTPCWM 188

Query: 202 PPD-----EEYSFKSDIWGIGVLAMEITWGGPPII---DPRILESQLFNISRRFPYESKT 261
            P+       Y FK+D+W  G+ A+E+  G  P       ++L   L N      YE   
Sbjct: 189 APEVMQQLHGYDFKADVWSFGITALELAHGHAPFSKYPPMKVLLMTLQNAPPGLDYER-- 248

Query: 262 EKIGDRRFFGDSVKEFVKSCLVKDRFRRPTASSLLTHPFVKKGEKPNFLFRSIPKGMINI 321
               D+R F  + KE V +CLVKD  +RPT+  LL HPF K     ++L ++I  G+  +
Sbjct: 249 ----DKR-FSKAFKEMVGTCLVKDPKKRPTSEKLLKHPFFKHARPADYLVKTILNGLPPL 308

Query: 322 DKKFEQEKERIIGFMKND----EKLKSESDKTMKISGWDYN 343
             ++ Q K +    +  +    E   S+ +    IS W++N
Sbjct: 309 GDRYRQIKSKEADLLMQNKSEYEAHLSQQEYIRGISAWNFN 338

[Close]

BLAST of Lsi09G012550 vs. TAIR 10
Match: AT1G23700.1 (Protein kinase superfamily protein )

HSP 1 Score: 162.2 bits (409), Expect = 1.2e-39
Identity = 109/330 (33.03%), Postives = 174/330 (52.73%), Query Frame = 0


Query: 22  FPTTSDGYTIITQIGASPHSIVYMAHCKSEDSTTITVAVKIMDI-NINLDLNSLNAS--- 81
           FP  +  Y I+ +IG      VY A C   D     VA+KI ++     DL ++      
Sbjct: 9   FPLVAKDYEILEEIGDG----VYRARCILLDE---IVAIKIWNLEKCTNDLETIRKEVHR 68

Query: 82  LSLINHPNILRPHCCFVGDDGRIWIVMPFIPTGPLQSVISHSFPRGMPDQFTSILLNQLL 141
           LSLI+HPN+LR HC F+ D   +WIVMPF+  G   +++   +P G+ +   +ILL ++L
Sbjct: 69  LSLIDHPNLLRVHCSFI-DSSSLWIVMPFMSCGSSLNIMKSVYPNGLEEPVIAILLREIL 128

Query: 142 RALVYLHEMGFVHKAIKTSNIFVDSKGCAKLSVFDIMLSKNNTNEIDHDSS------IPY 201
           +ALVYLH +G +H+ +K  N+ VDS+G  KL  F++  S  ++ E    SS       P 
Sbjct: 129 KALVYLHGLGHIHRNVKAGNVLVDSEGTVKLGDFEVSASMFDSVERMRTSSENTFVGNPR 188

Query: 202 WVPPDEE------YSFKSDIWGIGVLAMEITWG-GPPIIDPRILESQLFNISRRFPYESK 261
            + P+++      Y FK DIW  G+ A+E+  G  P  + P  L++  F       YE  
Sbjct: 189 RMAPEKDMQQVDGYDFKVDIWSFGMTALELAHGHSPTTVLPLNLQNSPFP-----NYEED 248

Query: 262 TEKIGDRRFFGDSVKEFVKSCLVKDRFRRPTASSLLTHPFVKKGEKPNFLFRSIPKGMIN 321
           T+       F  S +E V +CL++D  +RPTAS LL +PF+++     +L  +   G+  
Sbjct: 249 TK-------FSKSFRELVAACLIEDPEKRPTASQLLEYPFLQQTLSTEYLASTFLDGLSP 308

Query: 322 ID---KKFEQEKERII-GFMKNDEKLKSES 331
           +    +K ++EK +++ G   N EK+  E+
Sbjct: 309 LGERYRKLKEEKAKLVKGVDGNKEKVSQEN 318

[Close]

BLAST of Lsi09G012550 vs. TAIR 10
Match: AT1G79640.1 (Protein kinase superfamily protein )

HSP 1 Score: 157.1 bits (396), Expect = 3.7e-38
Identity = 96/343 (27.99%), Postives = 175/343 (51.02%), Query Frame = 0


Query: 22  FPTTSDGYTIITQIGASPHSIVYMAHCKSEDSTTITVAVKIMDI-NINLDLNSLN---AS 81
           +P   + YT+   IG    ++V+ A C   D     VA+KI+D    N DLN+++    +
Sbjct: 9   YPIGPEHYTLYEFIGQGVSALVHRALCIPFDE---VVAIKILDFERDNCDLNNISREAQT 68

Query: 82  LSLINHPNILRPHCCFVGDDGRIWIVMPFIPTGPLQSVISHSFPRGMPDQFTSILLNQLL 141
           + L++HPN+L+ HC FV D   +W++MP++  G    ++  ++P G  +   + +L + L
Sbjct: 69  MMLVDHPNVLKSHCSFVSDH-NLWVIMPYMSGGSCLHILKAAYPDGFEEAIIATILREAL 128

Query: 142 RALVYLHEMGFVHKAIKTSNIFVDSKGCAKLSVFDIMLSKNNTNEIDHDSS----IPYWV 201
           + L YLH+ G +H+ +K  NI + ++G  KL  F +     ++ +     +     P W+
Sbjct: 129 KGLDYLHQHGHIHRDVKAGNILLGARGAVKLGDFGVSACLFDSGDRQRTRNTFVGTPCWM 188

Query: 202 PPD-----EEYSFKSDIWGIGVLAMEITWGGPPII---DPRILESQLFNISRRFPYESKT 261
            P+       Y FK+DIW  G+  +E+  G  P       ++L   L N      YE   
Sbjct: 189 APEVMEQLHGYDFKADIWSFGITGLELAHGHAPFSKYPPMKVLLMTLQNAPPGLDYER-- 248

Query: 262 EKIGDRRFFGDSVKEFVKSCLVKDRFRRPTASSLLTHPFVKKGEKPNFLFRSIPKG---M 321
               D++ F  S K+ + SCLVKD  +RP+A  LL H F K+    +++ R +  G   +
Sbjct: 249 ----DKK-FSRSFKQMIASCLVKDPSKRPSAKKLLKHSFFKQARSSDYIARKLLDGLPDL 308

Query: 322 INIDKKFEQEKERIIGFMK---NDEKLKSESDKTMKISGWDYN 343
           +N  +  ++++E ++   K    +++  S+++    ISGW++N
Sbjct: 309 VNRVQAIKRKEEDMLAQEKMADGEKEELSQNEYKRGISGWNFN 340

[Close]

BLAST of Lsi09G012550 vs. TAIR 10
Match: AT1G79640.2 (Protein kinase superfamily protein )

HSP 1 Score: 157.1 bits (396), Expect = 3.7e-38
Identity = 96/343 (27.99%), Postives = 175/343 (51.02%), Query Frame = 0


Query: 22  FPTTSDGYTIITQIGASPHSIVYMAHCKSEDSTTITVAVKIMDI-NINLDLNSLN---AS 81
           +P   + YT+   IG    ++V+ A C   D     VA+KI+D    N DLN+++    +
Sbjct: 6   YPIGPEHYTLYEFIGQGVSALVHRALCIPFDE---VVAIKILDFERDNCDLNNISREAQT 65

Query: 82  LSLINHPNILRPHCCFVGDDGRIWIVMPFIPTGPLQSVISHSFPRGMPDQFTSILLNQLL 141
           + L++HPN+L+ HC FV D   +W++MP++  G    ++  ++P G  +   + +L + L
Sbjct: 66  MMLVDHPNVLKSHCSFVSDH-NLWVIMPYMSGGSCLHILKAAYPDGFEEAIIATILREAL 125

Query: 142 RALVYLHEMGFVHKAIKTSNIFVDSKGCAKLSVFDIMLSKNNTNEIDHDSS----IPYWV 201
           + L YLH+ G +H+ +K  NI + ++G  KL  F +     ++ +     +     P W+
Sbjct: 126 KGLDYLHQHGHIHRDVKAGNILLGARGAVKLGDFGVSACLFDSGDRQRTRNTFVGTPCWM 185

Query: 202 PPD-----EEYSFKSDIWGIGVLAMEITWGGPPII---DPRILESQLFNISRRFPYESKT 261
            P+       Y FK+DIW  G+  +E+  G  P       ++L   L N      YE   
Sbjct: 186 APEVMEQLHGYDFKADIWSFGITGLELAHGHAPFSKYPPMKVLLMTLQNAPPGLDYER-- 245

Query: 262 EKIGDRRFFGDSVKEFVKSCLVKDRFRRPTASSLLTHPFVKKGEKPNFLFRSIPKG---M 321
               D++ F  S K+ + SCLVKD  +RP+A  LL H F K+    +++ R +  G   +
Sbjct: 246 ----DKK-FSRSFKQMIASCLVKDPSKRPSAKKLLKHSFFKQARSSDYIARKLLDGLPDL 305

Query: 322 INIDKKFEQEKERIIGFMK---NDEKLKSESDKTMKISGWDYN 343
           +N  +  ++++E ++   K    +++  S+++    ISGW++N
Sbjct: 306 VNRVQAIKRKEEDMLAQEKMADGEKEELSQNEYKRGISGWNFN 337

The following BLAST results are available for this feature:

BLAST of Lsi09G012550 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O23304	4.0e-45	28.91	Serine/threonine-protein kinase BLUS1 OS=Arabidopsis thaliana OX=3702 GN=BLUS1 P...	[more]
Q551H4	2.2e-35	27.17	Serine/threonine-protein kinase fray2 OS=Dictyostelium discoideum OX=44689 GN=fr...	[more]
P97820	3.1e-21	23.54	Mitogen-activated protein kinase kinase kinase kinase 4 OS=Mus musculus OX=10090...	[more]
Q9JI10	1.2e-20	25.52	Serine/threonine-protein kinase 3 OS=Mus musculus OX=10090 GN=Stk3 PE=1 SV=1	[more]
P83510	4.4e-20	23.46	Traf2 and NCK-interacting protein kinase OS=Mus musculus OX=10090 GN=Tnik PE=1 S...	[more]

BLAST of Lsi09G012550 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3C8A9	7.0e-178	66.40	Serine/threonine-protein kinase BLUS1-like OS=Cucumis melo var. makuwa OX=119469...	[more]
A0A1S3CN88	7.0e-178	66.40	serine/threonine-protein kinase BLUS1-like OS=Cucumis melo OX=3656 GN=LOC1035028...	[more]
A0A6J1H6C5	9.6e-127	49.34	serine/threonine-protein kinase BLUS1-like OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A6J1KYZ4	4.0e-125	48.68	serine/threonine-protein kinase BLUS1-like OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A6J1CA92	4.2e-122	48.14	serine/threonine-protein kinase BLUS1-like OS=Momordica charantia OX=3673 GN=LOC...	[more]

BLAST of Lsi09G012550 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038875030.1	3.1e-204	73.41	serine/threonine-protein kinase BLUS1-like [Benincasa hispida]	[more]
XP_008465200.1	1.5e-177	66.40	PREDICTED: serine/threonine-protein kinase BLUS1-like [Cucumis melo] >KAA0057526...	[more]
XP_022958864.1	2.0e-126	49.34	serine/threonine-protein kinase BLUS1-like [Cucurbita moschata]	[more]
KAG6574930.1	2.0e-126	49.34	Serine/threonine-protein kinase BLUS1, partial [Cucurbita argyrosperma subsp. so...	[more]
KAG7013499.1	2.0e-126	49.62	Serine/threonine-protein kinase BLUS1, partial [Cucurbita argyrosperma subsp. ar...	[more]

BLAST of Lsi09G012550 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G14480.1	2.8e-46	28.91	Protein kinase superfamily protein	[more]
AT5G14720.1	7.2e-42	30.79	Protein kinase superfamily protein	[more]
AT1G23700.1	1.2e-39	33.03	Protein kinase superfamily protein	[more]
AT1G79640.1	3.7e-38	27.99	Protein kinase superfamily protein	[more]
AT1G79640.2	3.7e-38	27.99	Protein kinase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 457..491
None	No IPR available	COILS	Coil	Coil	coord: 365..392
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 25..324 e-value: 1.6E-45 score: 157.4
None	No IPR available	PIRSR	PIRSR630616-1	PIRSR630616-1	coord: 30..290 e-value: 4.4E-18 score: 63.1
None	No IPR available	PIRSR	PIRSR633573-1	PIRSR633573-1	coord: 29..220 e-value: 1.8E-6 score: 25.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 396..410
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 370..423
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 370..388
None	No IPR available	PANTHER	PTHR48014:SF7	SERINE/THREONINE-PROTEIN KINASE BLUS1	coord: 16..505
None	No IPR available	PANTHER	PTHR48014	SERINE/THREONINE-PROTEIN KINASE FRAY2	coord: 16..505
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 29..285 e-value: 1.5E-28 score: 99.9
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 29..285 score: 29.417814
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 24..296

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi09G012550.1	Lsi09G012550.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity