Lsi09G012350 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi09G012350
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptioncation/H(+) antiporter 15-like
Locationchr09: 19855207 .. 19858105 (+)
RNA-Seq ExpressionLsi09G012350
SyntenyLsi09G012350
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAGTAATGAAAACATGAATTTAGATGTTGAGTTGTTTTTTTACTAGATATAGACTGAGGGAAATGTTAATAAAAATATTTAAGGAAAAGAAAAGAAAAAGAAAGGAATAGGAAAATAGGTGGGGGTGTTTGGGTTGTAGGCCAGATCAGAATTGAAGTTGAATTTCTTCTAAATTTGTTAGTTCATGTTCTTCATTCAGGTTAAAACTCCTCCCATTAACCAATGCTTCTATACCTATCTTCCCTCCCTTTTGGTTTTCACTATCAATCTACTTTGCTCTCAAAATGGGTTCTATTATAATGGAACCAGACGACATTGCTGCTTACATTAGCGATGGAATCAGCAATACCTCTAAGAACTTTACCACAATATGTACATTTGCCAACCGAATTCACTACAATAACGACGTTTTCTCTGGAGAAAATCCTTTGGACTTCTCAGTTCCTCTTCTTTTCTTGCAACTTGGAATTTGTTCTGGAACTATCATCTTGTTTTCTCGATTTCTCAAGCCCCTCGGCCAACCCCTCATCGTCTCCCAAATTTTGGTAACTAACAACTCCTTTATTTTACATTCTGCCTATACATTTGACCAAAATTATTGATTGTGACTGGTGATTTGATTTTTTACCAGGGTGGTTTAATTTTAGGTTTTTCTGGTCTAAGCCACTTGGAGAAATTTAGAGAGACAATTTTTCCTCTCAGGGGATTCATTTTTCTTGATGTGGTTTCTGCACTTGGGCATATTTTTTACTTCTTTATGATTGGTGTGCAAACAGATATCTCGATGGTCAAGAAGATTGACAAGAAAACATTTAGTATTGGGTCATGTTCTGCGATTTTGGCTTTGATTCTCATCACCATTTACTCAATAATCTTCCCTAATTTTGTTGACATCCAAACATTCAATTACCTTATTGCCCTCGGTAGATTAGAGACATTTATCAACTTTCCCATGGTTTCATCCATTCTTCATGAGCTTCATTTGATAAACTCTGAATTTGGGAGAATGGCTTTGTCAACATCCATGGCTTCATCTATCTTCGGCATGGGTTTTTCATTTCTAGGAGGTATCTTCGCTCCAACTAGTAAGGCCAGACATGCCACCTTATCAAAAACTTTGACTATAATGGTGCTTACAGTTTTCGTCATTTTTATTTTTCGACATGTCACTCTATGGATGATAGAGAAGAATCCAATCGGACAACCATTGAAGGAAAGTTTTGTGATTACATTACTTTTAGGGGTGTTGATGATTGCCTTCTGCAGCCAAGTCTTTGGTTTGCCAATCTATATTGGTCCTTTTATTCTTGGATTTACAATACCTTCAGGGCCTCCCATTGGATCAACATTGGTGGAGAGACTTGATTTCATCACCTGTTGGGTTTTCATGCCCATCTTCTTTGCCAAAATAGGCTTGGTTGTCAATATCTACACTATCAAACTCATAAATTTCGTATGCATGTCATTCATTGTCTTTGTTAGTGCATTGGGAAAATTTTTGGGTGCTCTAATGATTTCAATATACTGCAAACTTCCTATGAGAGATGCTGTTTCACTCGGCCTCATCTTGAATAGTCAAGGAGCTCTCGAGCTTGGTATATTTAAAATTAAGAGGAGAGAAAAGGTAGGAAATTGCAATTCTTATTGACACCATCATTATTTTAATTGCATCTTATTATTTCGAAAAAAACTCATAGTAATTTGTTTACCTAAATCTGTACAGTTGATAGATGATGAAGCATTTGCAGTCGCATGCATATGCATGTTGGTTATTGTTGCAATTATCACTCCGATAATAAGATATCTCCTTGATCCTTCTAGAAGGTATATAGTTCACGGGAGAAGAACAATGATGCACTCAAGACCAGAGTCTGATCTTTGTATATTAGTATGCATTCACGACCAAGAAGATGTTCCAAGTGCCATTAGCCTACTTGATGCCTTGAATCCAACGAGACGAAGCCACCTTGTTGTTTACATGCTTCATCTTGTTGAGCTTCTCAGTCGTGCTCAACCAAAACTCATACACCACAAGCTTATGGAGGCTAGGGCTTTAAGCTCATACTCTTCTGAGCCTATTCTTAATGCCTTCAAATACTTTGGAGAGATAAATAGTGGAATTGTTGCAATTAATTCCTTCACTGCGATATCACCTTTTGCAACAATTCATGATGATGTTTGTTCACTTGCATTGGACAAAAGGTCCTCCTTGATTCTTGTTCCTTTTCACAAGAGATTTCATTCTAATGGCGTGATGTCATCGTCCAAACATAAAATAAAAACTGTTAACCAACATATCCTCGAGAAGGCACCATGCTCTGTTGCCCTTGTTGTTGAACGTAGATTTTTAAAGGTCTCACAAACTATTAAAAACAATTTGAATTCTTTTCAAATAGCTATAGTCTTCATAGGTGGACCAGACGATCATGAGGCAATGTTTATTGGGGCAAGAATGGTTGGGCATCCCAATATAAACTTGACAATGATTCGACTGTTAGAGAACGAGAATGTCCAAAATAACGACATTAAGGAAAGGAGGCTCGATGATGAAGCAGTGGCTGAGTTTCGACAAACTGTAGGAGACAACTACAGAGTAAGGTACATAGAAGAGGTGGTGAATGATGGCATTGGCACAATCTGTATATTACGTTCGATGGGGAGCAATTTTGATCTCGTAATGGTTGGAAGACGACATAGCCCATATTTGGCGTTGGTTCAAGGCTTGGTGCTTTGGAATGAGTGCACAGAACTTGGGGCAATTGGAGAGGTATTGGCATCTTCAAATTTCATGGGTGATGCCATGATATTGGTTGTGCAACAACATACAAGAGTGGCTAATGAAGACCAAGAGAATCATAAAGAGACTATCATTTCCATGGATAATATTGAAGTGTAA

mRNA sequence

ATGCAGTTAAAACTCCTCCCATTAACCAATGCTTCTATACCTATCTTCCCTCCCTTTTGGTTTTCACTATCAATCTACTTTGCTCTCAAAATGGGTTCTATTATAATGGAACCAGACGACATTGCTGCTTACATTAGCGATGGAATCAGCAATACCTCTAAGAACTTTACCACAATATGTACATTTGCCAACCGAATTCACTACAATAACGACGTTTTCTCTGGAGAAAATCCTTTGGACTTCTCAGTTCCTCTTCTTTTCTTGCAACTTGGAATTTGTTCTGGAACTATCATCTTGTTTTCTCGATTTCTCAAGCCCCTCGGCCAACCCCTCATCGTCTCCCAAATTTTGGGTGGTTTAATTTTAGGTTTTTCTGGTCTAAGCCACTTGGAGAAATTTAGAGAGACAATTTTTCCTCTCAGGGGATTCATTTTTCTTGATGTGGTTTCTGCACTTGGGCATATTTTTTACTTCTTTATGATTGGTGTGCAAACAGATATCTCGATGGTCAAGAAGATTGACAAGAAAACATTTAGTATTGGGTCATGTTCTGCGATTTTGGCTTTGATTCTCATCACCATTTACTCAATAATCTTCCCTAATTTTGTTGACATCCAAACATTCAATTACCTTATTGCCCTCGGTAGATTAGAGACATTTATCAACTTTCCCATGGTTTCATCCATTCTTCATGAGCTTCATTTGATAAACTCTGAATTTGGGAGAATGGCTTTGTCAACATCCATGGCTTCATCTATCTTCGGCATGGGTTTTTCATTTCTAGGAGGTATCTTCGCTCCAACTAGTAAGGCCAGACATGCCACCTTATCAAAAACTTTGACTATAATGGTGCTTACAGTTTTCGTCATTTTTATTTTTCGACATGTCACTCTATGGATGATAGAGAAGAATCCAATCGGACAACCATTGAAGGAAAGTTTTGTGATTACATTACTTTTAGGGGTGTTGATGATTGCCTTCTGCAGCCAAGTCTTTGGTTTGCCAATCTATATTGGTCCTTTTATTCTTGGATTTACAATACCTTCAGGGCCTCCCATTGGATCAACATTGGTGGAGAGACTTGATTTCATCACCTGTTGGGTTTTCATGCCCATCTTCTTTGCCAAAATAGGCTTGGTTGTCAATATCTACACTATCAAACTCATAAATTTCGTATGCATGTCATTCATTGTCTTTGTTAGTGCATTGGGAAAATTTTTGGGTGCTCTAATGATTTCAATATACTGCAAACTTCCTATGAGAGATGCTGTTTCACTCGGCCTCATCTTGAATAGTCAAGGAGCTCTCGAGCTTGGTATATTTAAAATTAAGAGGAGAGAAAAGTTGATAGATGATGAAGCATTTGCAGTCGCATGCATATGCATGTTGGTTATTGTTGCAATTATCACTCCGATAATAAGATATCTCCTTGATCCTTCTAGAAGGTATATAGTTCACGGGAGAAGAACAATGATGCACTCAAGACCAGAGTCTGATCTTTGTATATTAGTATGCATTCACGACCAAGAAGATGTTCCAAGTGCCATTAGCCTACTTGATGCCTTGAATCCAACGAGACGAAGCCACCTTGTTGTTTACATGCTTCATCTTGTTGAGCTTCTCAGTCGTGCTCAACCAAAACTCATACACCACAAGCTTATGGAGGCTAGGGCTTTAAGCTCATACTCTTCTGAGCCTATTCTTAATGCCTTCAAATACTTTGGAGAGATAAATAGTGGAATTGTTGCAATTAATTCCTTCACTGCGATATCACCTTTTGCAACAATTCATGATGATGTTTGTTCACTTGCATTGGACAAAAGGTCCTCCTTGATTCTTGTTCCTTTTCACAAGAGATTTCATTCTAATGGCGTGATGTCATCGTCCAAACATAAAATAAAAACTGTTAACCAACATATCCTCGAGAAGGCACCATGCTCTGTTGCCCTTGTTGTTGAACGTAGATTTTTAAAGGTCTCACAAACTATTAAAAACAATTTGAATTCTTTTCAAATAGCTATAGTCTTCATAGGTGGACCAGACGATCATGAGGCAATGTTTATTGGGGCAAGAATGGTTGGGCATCCCAATATAAACTTGACAATGATTCGACTGTTAGAGAACGAGAATGTCCAAAATAACGACATTAAGGAAAGGAGGCTCGATGATGAAGCAGTGGCTGAGTTTCGACAAACTGTAGGAGACAACTACAGAGTAAGGTACATAGAAGAGGTGGTGAATGATGGCATTGGCACAATCTGTATATTACGTTCGATGGGGAGCAATTTTGATCTCGTAATGGTTGGAAGACGACATAGCCCATATTTGGCGTTGGTTCAAGGCTTGGTGCTTTGGAATGAGTGCACAGAACTTGGGGCAATTGGAGAGGTATTGGCATCTTCAAATTTCATGGGTGATGCCATGATATTGGTTGTGCAACAACATACAAGAGTGGCTAATGAAGACCAAGAGAATCATAAAGAGACTATCATTTCCATGGATAATATTGAAGTGTAA

Coding sequence (CDS)

ATGCAGTTAAAACTCCTCCCATTAACCAATGCTTCTATACCTATCTTCCCTCCCTTTTGGTTTTCACTATCAATCTACTTTGCTCTCAAAATGGGTTCTATTATAATGGAACCAGACGACATTGCTGCTTACATTAGCGATGGAATCAGCAATACCTCTAAGAACTTTACCACAATATGTACATTTGCCAACCGAATTCACTACAATAACGACGTTTTCTCTGGAGAAAATCCTTTGGACTTCTCAGTTCCTCTTCTTTTCTTGCAACTTGGAATTTGTTCTGGAACTATCATCTTGTTTTCTCGATTTCTCAAGCCCCTCGGCCAACCCCTCATCGTCTCCCAAATTTTGGGTGGTTTAATTTTAGGTTTTTCTGGTCTAAGCCACTTGGAGAAATTTAGAGAGACAATTTTTCCTCTCAGGGGATTCATTTTTCTTGATGTGGTTTCTGCACTTGGGCATATTTTTTACTTCTTTATGATTGGTGTGCAAACAGATATCTCGATGGTCAAGAAGATTGACAAGAAAACATTTAGTATTGGGTCATGTTCTGCGATTTTGGCTTTGATTCTCATCACCATTTACTCAATAATCTTCCCTAATTTTGTTGACATCCAAACATTCAATTACCTTATTGCCCTCGGTAGATTAGAGACATTTATCAACTTTCCCATGGTTTCATCCATTCTTCATGAGCTTCATTTGATAAACTCTGAATTTGGGAGAATGGCTTTGTCAACATCCATGGCTTCATCTATCTTCGGCATGGGTTTTTCATTTCTAGGAGGTATCTTCGCTCCAACTAGTAAGGCCAGACATGCCACCTTATCAAAAACTTTGACTATAATGGTGCTTACAGTTTTCGTCATTTTTATTTTTCGACATGTCACTCTATGGATGATAGAGAAGAATCCAATCGGACAACCATTGAAGGAAAGTTTTGTGATTACATTACTTTTAGGGGTGTTGATGATTGCCTTCTGCAGCCAAGTCTTTGGTTTGCCAATCTATATTGGTCCTTTTATTCTTGGATTTACAATACCTTCAGGGCCTCCCATTGGATCAACATTGGTGGAGAGACTTGATTTCATCACCTGTTGGGTTTTCATGCCCATCTTCTTTGCCAAAATAGGCTTGGTTGTCAATATCTACACTATCAAACTCATAAATTTCGTATGCATGTCATTCATTGTCTTTGTTAGTGCATTGGGAAAATTTTTGGGTGCTCTAATGATTTCAATATACTGCAAACTTCCTATGAGAGATGCTGTTTCACTCGGCCTCATCTTGAATAGTCAAGGAGCTCTCGAGCTTGGTATATTTAAAATTAAGAGGAGAGAAAAGTTGATAGATGATGAAGCATTTGCAGTCGCATGCATATGCATGTTGGTTATTGTTGCAATTATCACTCCGATAATAAGATATCTCCTTGATCCTTCTAGAAGGTATATAGTTCACGGGAGAAGAACAATGATGCACTCAAGACCAGAGTCTGATCTTTGTATATTAGTATGCATTCACGACCAAGAAGATGTTCCAAGTGCCATTAGCCTACTTGATGCCTTGAATCCAACGAGACGAAGCCACCTTGTTGTTTACATGCTTCATCTTGTTGAGCTTCTCAGTCGTGCTCAACCAAAACTCATACACCACAAGCTTATGGAGGCTAGGGCTTTAAGCTCATACTCTTCTGAGCCTATTCTTAATGCCTTCAAATACTTTGGAGAGATAAATAGTGGAATTGTTGCAATTAATTCCTTCACTGCGATATCACCTTTTGCAACAATTCATGATGATGTTTGTTCACTTGCATTGGACAAAAGGTCCTCCTTGATTCTTGTTCCTTTTCACAAGAGATTTCATTCTAATGGCGTGATGTCATCGTCCAAACATAAAATAAAAACTGTTAACCAACATATCCTCGAGAAGGCACCATGCTCTGTTGCCCTTGTTGTTGAACGTAGATTTTTAAAGGTCTCACAAACTATTAAAAACAATTTGAATTCTTTTCAAATAGCTATAGTCTTCATAGGTGGACCAGACGATCATGAGGCAATGTTTATTGGGGCAAGAATGGTTGGGCATCCCAATATAAACTTGACAATGATTCGACTGTTAGAGAACGAGAATGTCCAAAATAACGACATTAAGGAAAGGAGGCTCGATGATGAAGCAGTGGCTGAGTTTCGACAAACTGTAGGAGACAACTACAGAGTAAGGTACATAGAAGAGGTGGTGAATGATGGCATTGGCACAATCTGTATATTACGTTCGATGGGGAGCAATTTTGATCTCGTAATGGTTGGAAGACGACATAGCCCATATTTGGCGTTGGTTCAAGGCTTGGTGCTTTGGAATGAGTGCACAGAACTTGGGGCAATTGGAGAGGTATTGGCATCTTCAAATTTCATGGGTGATGCCATGATATTGGTTGTGCAACAACATACAAGAGTGGCTAATGAAGACCAAGAGAATCATAAAGAGACTATCATTTCCATGGATAATATTGAAGTGTAA

Protein sequence

MQLKLLPLTNASIPIFPPFWFSLSIYFALKMGSIIMEPDDIAAYISDGISNTSKNFTTICTFANRIHYNNDVFSGENPLDFSVPLLFLQLGICSGTIILFSRFLKPLGQPLIVSQILGGLILGFSGLSHLEKFRETIFPLRGFIFLDVVSALGHIFYFFMIGVQTDISMVKKIDKKTFSIGSCSAILALILITIYSIIFPNFVDIQTFNYLIALGRLETFINFPMVSSILHELHLINSEFGRMALSTSMASSIFGMGFSFLGGIFAPTSKARHATLSKTLTIMVLTVFVIFIFRHVTLWMIEKNPIGQPLKESFVITLLLGVLMIAFCSQVFGLPIYIGPFILGFTIPSGPPIGSTLVERLDFITCWVFMPIFFAKIGLVVNIYTIKLINFVCMSFIVFVSALGKFLGALMISIYCKLPMRDAVSLGLILNSQGALELGIFKIKRREKLIDDEAFAVACICMLVIVAIITPIIRYLLDPSRRYIVHGRRTMMHSRPESDLCILVCIHDQEDVPSAISLLDALNPTRRSHLVVYMLHLVELLSRAQPKLIHHKLMEARALSSYSSEPILNAFKYFGEINSGIVAINSFTAISPFATIHDDVCSLALDKRSSLILVPFHKRFHSNGVMSSSKHKIKTVNQHILEKAPCSVALVVERRFLKVSQTIKNNLNSFQIAIVFIGGPDDHEAMFIGARMVGHPNINLTMIRLLENENVQNNDIKERRLDDEAVAEFRQTVGDNYRVRYIEEVVNDGIGTICILRSMGSNFDLVMVGRRHSPYLALVQGLVLWNECTELGAIGEVLASSNFMGDAMILVVQQHTRVANEDQENHKETIISMDNIEV
Homology
BLAST of Lsi09G012350 vs. ExPASy Swiss-Prot
Match: Q9SIT5 (Cation/H(+) antiporter 15 OS=Arabidopsis thaliana OX=3702 GN=CHX15 PE=2 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 5.1e-135
Identity = 285/805 (35.40%), Postives = 471/805 (58.51%), Query Frame = 0

Query: 58  TICTFANRIHYNNDVFSGENPLDFSVPLLFLQLGICSGTIILFSRFLKPLGQPLIVSQIL 117
           +I  +A  +   N V+ G+NPLDFS+PL  LQL +       F   LKP  QP ++S+IL
Sbjct: 12  SIICYAPSMITTNGVWQGDNPLDFSLPLFVLQLTLVVVVTRFFVFILKPFRQPRVISEIL 71

Query: 118 GGLILGFSGLSHLEKFRETIFPLRGFIFLDVVSALGHIFYFFMIGVQTDISMVKKIDKKT 177
           GG++LG S L    KF  TIFP R  + L+ ++ +G +++ F++GV+ DI +V+K  K+ 
Sbjct: 72  GGIVLGPSVLGRSTKFAHTIFPQRSVMVLETMANVGLLYFLFLVGVEMDIMVVRKTGKRA 131

Query: 178 FSIGSCSAILALILITIYSIIFPNFVD-IQTFNYLIALGRLETFINFPMVSSILHELHLI 237
            +I     +L  ++   +S       D +    Y++ LG   +   FP+++ IL EL LI
Sbjct: 132 LTIAIGGMVLPFLIGAAFSFSMHRSEDHLGQGTYILFLGVALSVTAFPVLARILAELKLI 191

Query: 238 NSEFGRMALSTSMASSIFGMGFSFLGGIFAPTSKARHATLSKTLTIMVLTVFVIFIFRHV 297
           N+E GR+++S ++ + +F      L    A + K   A+L   ++  V     +F+ R  
Sbjct: 192 NTEIGRISMSAALVNDMFAWILLALAIALAESDKTSFASLWVMISSAVFIAVCVFVVRPG 251

Query: 298 TLWMIEKNPIGQPLKESFVITLLLGVLMIAFCSQVFGLPIYIGPFILGFTIPSGPPIGST 357
             W+I K P G+   E  +  +L GV++  F +   G     G F+ G  IP+G P+G T
Sbjct: 252 IAWIIRKTPEGENFSEFHICLILTGVMISGFITDAIGTHSVFGAFVFGLVIPNG-PLGLT 311

Query: 358 LVERLDFITCWVFMPIFFAKIGLVVNIYTIK-LINFVCMSFIVFVSALGKFLGALMISIY 417
           L+E+L+     + +P+FFA  GL  NI  I+    ++ +  ++F++  GK +G ++++ +
Sbjct: 312 LIEKLEDFVSGLLLPLFFAISGLKTNIAAIQGPATWLTLFLVIFLACAGKVIGTVIVAFF 371

Query: 418 CKLPMRDAVSLGLILNSQGALELGIFKIKRREKLIDDEAFAVACICMLVIVAIITPIIRY 477
             +P+R+ ++LGL+LN++G +E+ +  + + +K++DDE FA   +  LV+  +ITPI+  
Sbjct: 372 HGMPVREGITLGLLLNTKGLVEMIVLNVGKDQKVLDDETFATMVLVALVMTGVITPIVTI 431

Query: 478 LLDPSRRYIVHGRRTMMHSRPESDLCILVCIHDQEDVPSAISLLDALNPTRRSHLVVYML 537
           L  P ++ + + RRT+  ++P+S+L +LVC+H   +VP+ I+LL+A +PT+RS + +Y+L
Sbjct: 432 LYKPVKKSVSYKRRTIQQTKPDSELRVLVCVHTPRNVPTIINLLEASHPTKRSPICIYVL 491

Query: 538 HLVELLSRAQPKLIHHKLME----ARALSSYSSEPILNAFKYFGEINSGIVAINSFTAIS 597
           HLVEL  RA   LI H   +    A   +   S+ I+NAF+ + E ++  VA+   TAIS
Sbjct: 492 HLVELTGRASAMLIVHNTRKSGRPALNRTQAQSDHIINAFENY-EQHAAFVAVQPLTAIS 551

Query: 598 PFATIHDDVCSLALDKRSSLILVPFHKRFHSNGVMSSSKHKIKTVNQHILEKAPCSVALV 657
           P++T+H+DVCSLA DKR S I++PFHK+   +G M S+    + VNQ++LE +PCSV ++
Sbjct: 552 PYSTMHEDVCSLAEDKRVSFIIIPFHKQQTVDGGMESTNPAYRLVNQNLLENSPCSVGIL 611

Query: 658 VERRFLKVSQTIKNNLNSFQIAIVFIGGPDDHEAMFIGARMVGHPNINLTMIRLLENEN- 717
           V+R  L  +  + +N  S Q+A++F GGPDD EA+    RM  HP I LT++R + +E+ 
Sbjct: 612 VDRG-LNGATRLNSNTVSLQVAVLFFGGPDDREALAYAWRMAQHPGITLTVLRFIHDEDE 671

Query: 718 --------VQNNDI--------KERRLDDEAVAEFRQTVGDNYRVRYIEEVVNDGIGTIC 777
                     ++D+        K+R+LDD+ +  FR    +   + YIE++V++G  T+ 
Sbjct: 672 ADTASTRATNDSDLKIPKMDHRKQRQLDDDYINLFRAENAEYESIVYIEKLVSNGEETVA 731

Query: 778 ILRSMGSNFDLVMVGRRHSPYLALVQGLVLWNECTELGAIGEVLASSNFMGDAMILVVQQ 837
            +RSM S+ DL +VGR       L  GL  W+EC ELGAIG++LASS+F     +LVVQQ
Sbjct: 732 AVRSMDSSHDLFIVGRGEGMSSPLTAGLTDWSECPELGAIGDLLASSDFAATVSVLVVQQ 791

Query: 838 HT-RVANEDQENHKETIISMDNIEV 839
           +    A ED  +  E+ +     +V
Sbjct: 792 YVGSWAQEDDMDFPESPVHSHETKV 813

BLAST of Lsi09G012350 vs. ExPASy Swiss-Prot
Match: Q9LMJ1 (Cation/H(+) antiporter 14 OS=Arabidopsis thaliana OX=3702 GN=CHX14 PE=2 SV=1)

HSP 1 Score: 397.1 bits (1019), Expect = 4.8e-109
Identity = 237/769 (30.82%), Postives = 425/769 (55.27%), Query Frame = 0

Query: 64  NRIHYNNDVFSGENPLDFSVPLLFLQLGICSGTIILFSRFLKPLGQPLIVSQILGGLILG 123
           N +  +  VF G +PL +++PL+ LQ+ +   T  L  R LKPL Q +I +Q+L G+ILG
Sbjct: 31  NHMLTSKGVFLGSDPLKYAMPLMLLQMSVIIITSRLLYRLLKPLKQGMISAQVLAGIILG 90

Query: 124 FSGLSHLEKFRETIFPLRGFIFLDVVSALGHIFYFFMIGVQTDISMVKKIDKKTFSIGSC 183
            S       + +   P+ G I L  +S LG   + F++G++ D S+++K   K   IG+ 
Sbjct: 91  PSLFGQSSAYMQMFLPISGKITLQTLSNLGFFIHLFLLGLRIDASIIRKAGSKAILIGTA 150

Query: 184 SAILALILITIYSIIFPNFVDI--QTFNYLIALGRLETFINFPMVSSILHELHLINSEFG 243
           S  L   L  +  +   N  ++     + +  +  L    +FP+ +++L EL+++NS+ G
Sbjct: 151 SYALPFSLGNLTVLFLKNTYNLPPDVVHCISTVISLNAMTSFPVTTTVLAELNILNSDLG 210

Query: 244 RMALSTSMASSIFGMGFSFLGGIFAPTSKARHATLSKTLTIMVLTVFVIFIF---RHVTL 303
           R+A + S+    F    + +  +F      R  TL+   + + +T  ++ IF   R   +
Sbjct: 211 RLATNCSIVCEAFSWIVALVFRMF-----LRDGTLASVWSFVWVTALILVIFFVCRPAII 270

Query: 304 WMIEKNPIG-QPLKESFVITLLLGVLMIAFCSQVFGLPIYIGPFILGFTIPSGPPIGSTL 363
           W+ E+  I      E     +++ +L I+  S+V G+    G F LG ++P GPP+G+ L
Sbjct: 271 WLTERRSISIDKAGEIPFFPIIMVLLTISLTSEVLGVHAAFGAFWLGVSLPDGPPLGTGL 330

Query: 364 VERLDFITCWVFMPIFFAKIGLVVNIYTIKLINFVCMSFIVFVSALGKFLGALMISIYCK 423
             +L+     + +P F +  GL  N + I   +   +  ++ ++   KFLG    S YC 
Sbjct: 331 TTKLEMFATSLMLPCFISISGLQTNFFIIGESHVKIIEAVILITYGCKFLGTAAASAYCN 390

Query: 424 LPMRDAVSLGLILNSQGALELGIFKIKRREKLIDDEAFAVACICMLVIVAIITPIIRYLL 483
           + + DA SL L++  QG +E+    + + EK+++ E F +  I +L++  I   ++  L 
Sbjct: 391 IQIGDAFSLALLMCCQGVIEIYTCVMWKDEKVLNTECFNLLIITLLLVTGISRFLVVCLY 450

Query: 484 DPSRRYIVHGRRTMMHSRPES-DLCILVCIHDQEDVPSAISLLDALNPTRRSHLVVYMLH 543
           DPS+RY    +RT++ +R  +    +L+C+++ E+VPS ++LL+A  P+R S + V+ LH
Sbjct: 451 DPSKRYRSKSKRTILDTRQRNLQFRLLLCVYNVENVPSMVNLLEASYPSRFSPISVFTLH 510

Query: 544 LVELLSRAQPKLIHHKLMEARALSSYSSEPILNAFKYFGEINSGIVAINSFTAISPFATI 603
           LVEL  RA   L+ H  M     ++  S  I+N F+ F + N G +    FTA +PF++I
Sbjct: 511 LVELKGRAHAVLVPHHQMNKLDPNTVQSTHIVNGFQRFEQQNQGTLMAQHFTAAAPFSSI 570

Query: 604 HDDVCSLALDKRSSLILVPFHKRFHSNGVMSSSKHKIKTVNQHILEKAPCSVALVVERRF 663
           +DD+C+LALDK+++LI++PFHK++  +G +      I+ +N ++LEKAPCSV + ++R  
Sbjct: 571 NDDICTLALDKKATLIVIPFHKQYAIDGTVDHVNPSIRNINLNVLEKAPCSVGIFIDRGE 630

Query: 664 LKVSQTIKNNLNSFQIAIVFIGGPDDHEAMFIGARMVGHPNINLTMIRLLENENVQNN-- 723
            +  +++  +     +A++FI G DD EA+    R+  HP +++TMI      ++Q N  
Sbjct: 631 TEGRRSVLMSYTWRNVAVIFIEGRDDAEALAFSMRIAEHPEVSVTMIHFRHKSSLQQNHV 690

Query: 724 -DIKERRLDDEAVAEFRQTVGDNYRVRYIEEVVNDGIGTICILRSMGSNFDLVMVGRRHS 783
            D++    +   + +F+       ++ Y EE+V DG+ T  ++ S+G +FDLV+VGR H 
Sbjct: 691 VDVESELAESYLINDFKNFAMSKPKISYREEIVRDGVETTQVISSLGDSFDLVVVGRDHD 750

Query: 784 PYLALVQGLVLWNECTELGAIGEVLASSNFMGDAMILVVQQHTRVANED 823
              +++ GL  W+EC ELG IG++ ASS+F    +++  Q+   +A ++
Sbjct: 751 LESSVLYGLTDWSECPELGVIGDMFASSDFHFSVLVIHQQEGDSLAMDN 794

BLAST of Lsi09G012350 vs. ExPASy Swiss-Prot
Match: O22920 (Cation/H(+) symporter 13 OS=Arabidopsis thaliana OX=3702 GN=CHX13 PE=1 SV=1)

HSP 1 Score: 376.3 bits (965), Expect = 8.9e-103
Identity = 234/766 (30.55%), Postives = 423/766 (55.22%), Query Frame = 0

Query: 59  ICTFANRIHYNNDVFSGENPLDFSVPLLFLQLGICSGTIILFSRFLKPLGQPLIVSQILG 118
           +C   N +  +  +F   NPL +++PLL LQ+ +   T  L  R L+PL Q +I +Q+L 
Sbjct: 29  VCQAQNML-TSRGIFMKSNPLKYALPLLLLQMSVIIVTSRLIFRVLQPLKQGMISAQVLT 88

Query: 119 GLILGFSGLSHLEKFRETIFPLRGFIFLDVVSALGHIFYFFMIGVQTDISMVKKIDKKTF 178
           G++LG S L H   +     P  G I +  +S +G + + F++G++ D S+++K   K  
Sbjct: 89  GVVLGPSFLGHNVIYMNMFLPAGGKIIIQTLSNVGFVIHLFLLGLKIDGSIIRKAGSKAI 148

Query: 179 SIGSCSAILALIL--ITIYSIIFPNFVDIQTFNYLIALGRLETFINFPMVSSILHELHLI 238
            IG+ S      L  +TI  I     +     +   +   L +  +FP+ +++L EL+++
Sbjct: 149 LIGTASYAFPFSLGNLTIMFISKTMGLPSDVISCTSSAISLSSMTSFPVTTTVLAELNIL 208

Query: 239 NSEFGRMALSTSMASSIFGMGFSFLGGIFAPTSKARHATLSKTLTIMV-LTVFVIFIFRH 298
           NSE GR+A   SM   +      F+   F   ++ R  T    L++++ L + + F+FR 
Sbjct: 209 NSELGRLATHCSMVCEVCSW---FVALAFNLYTRDRTMTSLYALSMIIGLLLVIYFVFRP 268

Query: 299 VTLWMIEKNPIGQPLKE---SFVITLLLGVLMIAFCSQVFGLPIYIGPFILGFTIPSGPP 358
           + +W+ ++       K+    F + LLL +  ++   +  G+    G F LG ++P GPP
Sbjct: 269 IIVWLTQRKTKSMDKKDVVPFFPVLLLLSIASLS--GEAMGVHAAFGAFWLGVSLPDGPP 328

Query: 359 IGSTLVERLDFITCWVFMPIFFAKIGLVVNIYTIKLI---NFVCMSFIVFVSALGKFLGA 418
           +G+ L  +L+     +F+P F A  GL  N + I      + V +  I+ ++   KFLG 
Sbjct: 329 LGTELAAKLEMFASNLFLPCFIAISGLQTNFFEITESHEHHVVMIEIILLITYGCKFLGT 388

Query: 419 LMISIYCKLPMRDAVSLGLILNSQGALELGIFKIKRREKLIDDEAFAVACICMLVIVAII 478
              S YC+  + DA+ L  ++  QG +E+    + +  +++D E F +  I +L +  I 
Sbjct: 389 AAASAYCQTQIGDALCLAFLMCCQGIIEVYTTIVWKDAQVVDTECFNLVIITILFVTGIS 448

Query: 479 TPIIRYLLDPSRRYIVHGRRTMMHSRPES-DLCILVCIHDQEDVPSAISLLDALNPTRRS 538
             ++ YL DPS+RY    +RT++++R  +  L +L+ +++ E+VPS ++LL+A  PTR +
Sbjct: 449 RFLVVYLYDPSKRYKSKSKRTILNTRQHNLQLRLLLGLYNVENVPSMVNLLEATYPTRFN 508

Query: 539 HLVVYMLHLVELLSRAQPKLIHHKLMEARALSSYSSEPILNAFKYFGEINSGIVAINSFT 598
            +  + LHLVEL  RA   L  H  M     ++  S  I+NAF+ F +   G +    FT
Sbjct: 509 PISFFTLHLVELKGRAHALLTPHHQMNKLDPNTAQSTHIVNAFQRFEQKYQGALMAQHFT 568

Query: 599 AISPFATIHDDVCSLALDKRSSLILVPFHKRFHSNGVMSSSKHKIKTVNQHILEKAPCSV 658
           A +P+++I++D+C+LALDK+++LI++PFHK++  +G +      I+T+N ++L+ APCSV
Sbjct: 569 AAAPYSSINNDICTLALDKKATLIVIPFHKQYAIDGTVGQVNGPIRTINLNVLDAAPCSV 628

Query: 659 ALVVERRFLKVSQTIKNNLNSFQIAIVFIGGPDDHEAMFIGARMVGHPNINLTMIRLLEN 718
           A+ ++R   +  +++        +A++FIGG DD EA+ +  RM   P++N+TMI     
Sbjct: 629 AIFIDRGETEGRRSVLMTNTWQNVAMLFIGGKDDAEALALCMRMAEKPDLNVTMIHFRHK 688

Query: 719 ENVQNNDIKERRLDDEAVAEFRQTVGDNYRVRYIEEVVNDGIGTICILRSMGSNFDLVMV 778
             +Q+ D  +   +   +++F+    +  ++ Y+EE+V DG+ T  ++ S+G  +D+V+V
Sbjct: 689 SALQDEDYSDMS-EYNLISDFKSYAANKGKIHYVEEIVRDGVETTQVISSLGDAYDMVLV 748

Query: 779 GRRHSPYLALVQGLVLWNECTELGAIGEVLASSNFMGDAMILVVQQ 815
           GR H    +++ GL  W+EC ELG IG++L S +F    +++  QQ
Sbjct: 749 GRDHDLESSVLYGLTDWSECPELGVIGDMLTSPDFHFSVLVVHQQQ 787

BLAST of Lsi09G012350 vs. ExPASy Swiss-Prot
Match: Q9FFB8 (Cation/H(+) antiporter 3 OS=Arabidopsis thaliana OX=3702 GN=CHX3 PE=2 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 2.4e-100
Identity = 244/758 (32.19%), Postives = 419/758 (55.28%), Query Frame = 0

Query: 103 FLKPLGQPLIVSQILGGLILGFSGLSH---------LEKFRETIFPLRGFIFLDVVSALG 162
           FL+ LG     S +L G++L  S L            E ++E +F L         +A  
Sbjct: 75  FLRRLGMIRFTSHMLTGVLLSKSFLKENSAARRFFSTEDYKEIVFSL--------TAACS 134

Query: 163 HIFYFFMIGVQTDISMVKKIDKKTFSIGSCSAILALILITIYSIIFPNFVDIQTFNYLIA 222
           ++ ++F++GV+ D  +++   +K  +IG  S +L+ ++ ++  I F N  D+ T N    
Sbjct: 135 YMMFWFLMGVKMDTGLIRTTGRKAITIGLSSVLLSTLVCSV--IFFGNLRDVGTKNSDHT 194

Query: 223 LGRLETFI--------NFPMVSSILHELHLINSEFGRMALSTSMAS----SIFGMGFSF- 282
           L  LE  +        +FP+V ++L EL L NSE GR+A+S+++ S    SI      F 
Sbjct: 195 LNSLEYVVIYSIQCLSSFPVVGNLLFELRLQNSELGRLAISSAVISDFSTSILASVLIFM 254

Query: 283 ---------LGGIFAPTSKARHATLSKTLTIMVLTVFVIFIFRHVTLWMIEKNPIGQPLK 342
                    LG +F     A +  L +   +++     I++FR +  ++I++ P G+P+K
Sbjct: 255 KELKDEQTRLGSVFIGDVIAGNRPLMRAGIVVLFVCIAIYVFRPLMFYIIKQTPSGRPVK 314

Query: 343 ESFVITLLLGVLMIAFCSQVFGLPIYIGPFILGFTIPSGPPIGSTLVERLDFITCWVFMP 402
             ++ T+++ V   A  +      I++GPFILG  +P GPP+GS ++++ +      F+P
Sbjct: 315 AIYLSTIIVMVSGSAILANWCKQSIFMGPFILGLAVPHGPPLGSAIIQKYESAIFGTFLP 374

Query: 403 IFFAKIGLVVNIYTI---KLINFVCMSFIVFVSALGKFLGALMISIYCKLPMRDAVSLGL 462
            F A     ++I  +   + +N + +  I+  S + KF+   + +++  +PM D  +L L
Sbjct: 375 FFIASSSTEIDISALFGWEGLNGIIL--IMVTSFVVKFIFTTVPALFYGMPMEDCFALSL 434

Query: 463 ILNSQGALELGIFKIKRREKLIDDEAFAVACICMLVIVAIITPIIRYLLDPSRRYIVHGR 522
           I++ +G  ELG + +  +   +  E F VAC+ + +  AII PI+RYL DPSR Y  + +
Sbjct: 435 IMSFKGIFELGAYALAYQRGSVRPETFTVACLYITLNSAIIPPILRYLYDPSRMYAGYEK 494

Query: 523 RTMMHSRPESDLCILVCIHDQEDVPSAISLLDALNPTRRSHLVVYMLHLVELLSRAQPKL 582
           R M H +P S+L IL CI+  +D+   I+LL+A+ P+R S +  Y+LHL+EL+ +A P  
Sbjct: 495 RNMQHLKPNSELRILSCIYRTDDISPMINLLEAICPSRESPVATYVLHLMELVGQANPIF 554

Query: 583 IHHKLMEARALSSYSSEPILNAFKYFGEINSGIVAINSFTAISPFATIHDDVCSLALDKR 642
           I HKL   R   +  S  +L +F+ F +   G V ++++TA+S   T+H D+C LAL+  
Sbjct: 555 ISHKLQTRRTEETSYSNNVLVSFEKFRKDFYGSVFVSTYTALSMPDTMHGDICMLALNNT 614

Query: 643 SSLILVPFHKRFHSNG-VMSSSKHKIKTVNQHILEKAPCSVALVVER------RFLKVSQ 702
           +SLIL+PFH+ + ++G  + S+ + I+ +N+ +L+ APCSV + V R            +
Sbjct: 615 TSLILLPFHQTWSADGSALISNNNMIRNLNKSVLDVAPCSVGVFVYRSSSGRKNISSGRK 674

Query: 703 TIKN---NLNSFQIAIVFIGGPDDHEAMFIGARMVGHPNINLTMIRLL-ENENVQNNDIK 762
           TI     NL+S+ I ++F+GG DD EA+ +  RM   P IN+T++RL+  +E  + N + 
Sbjct: 675 TINGTVPNLSSYNICMIFLGGKDDREAVTLATRMARDPRINITIVRLITTDEKARENTVW 734

Query: 763 ERRLDDEAVAEFR-QTVGDNYRVRYIEEVVNDGIGTICILRSMGSNFDLVMVGRRHSPYL 815
           ++ LDDE + + +  T+ D   + Y E+ + D   T  +LRSM S+FD+ +VGR +    
Sbjct: 735 DKMLDDELLRDVKSNTLVD---IFYSEKAIEDAAETSSLLRSMVSDFDMFIVGRGNGRTS 794

BLAST of Lsi09G012350 vs. ExPASy Swiss-Prot
Match: Q9LUN4 (Cation/H(+) antiporter 19 OS=Arabidopsis thaliana OX=3702 GN=CHX19 PE=2 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 3.1e-100
Identity = 239/772 (30.96%), Postives = 414/772 (53.63%), Query Frame = 0

Query: 69  NNDVFSGENPLDFSVPLLFLQLGICSGTIILFSRFLKPLGQPLIVSQILGGLILGFSGLS 128
           +N  F  E+PLDF++PL+ LQ+ +      L + FLKPL QP ++++I+GG++LG S L 
Sbjct: 18  SNGAFQNESPLDFALPLIILQIVLVVVFTRLLAYFLKPLKQPRVIAEIIGGILLGPSALG 77

Query: 129 HLEKFRETIFPLRGFIFLDVVSALGHIFYFFMIGVQTDISMVKKIDKKTFSIGSCSAILA 188
             + + +TIFP +    LD ++ +G +F+ F++G++ D + +KK  KK+  I      L 
Sbjct: 78  RSKAYLDTIFPKKSLTVLDTLANIGLLFFLFLVGLELDFAAIKKTGKKSLLIAIAGISLP 137

Query: 189 LILITIYSIIFPNFVD--IQTFNYLIALGRLETFINFPMVSSILHELHLINSEFGRMALS 248
            I+    S +    +   +    +++ +G   +   FP+++ IL EL L+ ++ GRMA+S
Sbjct: 138 FIVGVGTSFVLSATISKGVDQLPFIVFMGVALSITAFPVLARILAELKLLTTDIGRMAMS 197

Query: 249 TSMASSIFGMGFSFLGGIFAPTSKARHATLSKTLTIMVLTVFVIF---IFRHVTLWMIEK 308
              A+ +  +    L  +    S    + L     ++  T FVIF     + +  +M  +
Sbjct: 198 ---AAGVNDVAAWILLALAIALSGDGTSPLVSVWVLLCGTGFVIFAVVAIKPLLAYMARR 257

Query: 309 NPIGQPLKESFVITLLLGVLMIAFCSQVFGLPIYIGPFILGFTIPSGPPIGSTLVERLDF 368
            P G+P+KE +V   L  VL  +F +   G+    G F++G   P   P    L E+++ 
Sbjct: 258 CPEGEPVKELYVCVTLTVVLAASFVTDTIGIHALFGAFVVGIVAPKEGPFCRILTEKIED 317

Query: 369 ITCWVFMPIFFAKIGLVVNIYTIK-LINFVCMSFIVFVSALGKFLGALMISIYCKLPMRD 428
           +   + +P++FA  GL  ++ TI+   ++  +  ++  +  GK +G +  S+ CK+P R+
Sbjct: 318 LVSGLLLPLYFAASGLKTDVTTIRGAQSWGLLVLVILTTCFGKIVGTVGSSMLCKVPFRE 377

Query: 429 AVSLGLILNSQGALELGIFKIKRREKLIDDEAFAVACICMLVIVAIITPIIRYLLDPSRR 488
           AV+LG ++N++G +EL +  I +  K+++D+AFA+  +  L    I TPI+  +  P+R+
Sbjct: 378 AVTLGFLMNTKGLVELIVLNIGKDRKVLNDQAFAILVLMALFTTFITTPIVMLIYKPARK 437

Query: 489 YIVHGRRTMMHSRPESDLCILVCIHDQEDVPSAISLLDALNPT-RRSHLVVYMLHLVELL 548
              +  RT+     +S+L IL C H   ++P+ I+L+++   T ++  L VY +HL+EL 
Sbjct: 438 GAPYKHRTIQRKDHDSELRILACFHSTRNIPTLINLIESSRGTGKKGRLCVYAMHLMELS 497

Query: 549 SRAQPKLIHHKL----MEARALSSYSSEPILNAFKYFGEINSGIVAINSFTAISPFATIH 608
            R+    + HK     +        S++ ++ AF+ +  + +  VA+   TAIS  ++IH
Sbjct: 498 ERSSAIAMVHKARNNGLPIWNKIERSTDQMVIAFEAYQHLRA--VAVRPMTAISGLSSIH 557

Query: 609 DDVCSLALDKRSSLILVPFHKRFHSNGVMSSSKHKIKTVNQHILEKAPCSVALVVERRFL 668
           +D+C+ A  KR ++IL+PFHK    +G M S  H+   VNQ +L++APCSV ++V+R   
Sbjct: 558 EDICTSAHQKRVAMILLPFHKHQRMDGAMESIGHRFHEVNQRVLQRAPCSVGILVDRGLG 617

Query: 669 KVSQTIKNNLNSFQIAIVFIGGPDDHEAMFIGARMVGHPNINLTM---------IRLLEN 728
             SQ + + + ++++ I F GG DD EA+  G +MV HP I LT+         ++  E 
Sbjct: 618 GTSQVVASEV-AYKVVIPFFGGLDDREALAYGMKMVEHPGITLTVYKFVAARGTLKRFEK 677

Query: 729 ENVQNNDIKERRLDDEAVAEFRQTVGDNYRVRYIEEVVNDGIGTICILRSMGSNFDLVMV 788
                 + KE+  D+E V E       N  + Y E VV      I  L+SM S  +L +V
Sbjct: 678 SEHDEKEKKEKETDEEFVRELMNDPRGNESLAYEERVVESKDDIIATLKSM-SKCNLFVV 737

Query: 789 GRRHSPYLALVQGLVLWNECTELGAIGEVLASSNFMGDAMILVVQQHTRVAN 821
           GR      A V  LV   +C ELG +G +L+SS F   A +LVVQ +   A+
Sbjct: 738 GRN-----AAVASLVKSTDCPELGPVGRLLSSSEFSTTASVLVVQGYDPAAD 777

BLAST of Lsi09G012350 vs. ExPASy TrEMBL
Match: A0A1S3BNS6 (cation/H(+) antiporter 15-like OS=Cucumis melo OX=3656 GN=LOC103491876 PE=4 SV=1)

HSP 1 Score: 1119.8 bits (2895), Expect = 0.0e+00
Identity = 575/803 (71.61%), Postives = 680/803 (84.68%), Query Frame = 0

Query: 31  MGSIIMEPDDIAAYISDGISN-TSKNFTTICTFANRIHYNNDVFSGENPLDFSVPLLFLQ 90
           MGSI+MEP+DIA YI+ GI +  +KNFTTICTFAN IH  + +F G NPL+FSVPLLFLQ
Sbjct: 1   MGSIVMEPNDIATYINGGIGHRPTKNFTTICTFANHIHCTS-IFIGANPLEFSVPLLFLQ 60

Query: 91  LGICSGTIILFSRFLKPLGQPLIVSQILGGLILGFSGLSHLEKFRETIFPLRGFIFLDVV 150
           LGICSGTIILFS+ LKPLGQPLIVSQILGGL+LG +GLS++E F+ETIFPLRGF+ LDVV
Sbjct: 61  LGICSGTIILFSQLLKPLGQPLIVSQILGGLVLGSTGLSNVEIFKETIFPLRGFVSLDVV 120

Query: 151 SALGHIFYFFMIGVQTDISMVKKIDKKTFSIGSCSAILALILITIYSIIFPNFVDIQTFN 210
           SALGHIFY+F+IGVQTDIS+VK ID KTF IGSC+ ILA +L+ IYS+   + +D+  F 
Sbjct: 121 SALGHIFYYFLIGVQTDISIVKNIDMKTFGIGSCATILATLLVIIYSMSLASIIDMTKFK 180

Query: 211 YLIALGRLETFINFPMVSSILHELHLINSEFGRMALSTSMASSIFGMGFSFLGGIFAPTS 270
           Y+  LG+LE+FINFPMV+S+++ELHL+NSEFGR++L++SMASS+  +  + LG I +P  
Sbjct: 181 YIFELGKLESFINFPMVASLVYELHLVNSEFGRISLTSSMASSLLSVSLTLLGSILSPNG 240

Query: 271 KARHATLSKTLTIMVLTVFVIFIFRHVTLWMIEKNPIGQPLKESFVITLLLGVLMIAFCS 330
             R   LS+T  IMVL + ++F  R  TLWM++ NPIGQPLKE FVITLLLGVL++AFC 
Sbjct: 241 TTRQQILSETFAIMVLILVIVFSIRPATLWMVKMNPIGQPLKECFVITLLLGVLVVAFCC 300

Query: 331 QVFGLPIYIGPFILGFTIPSGPPIGSTLVERLDFITCWVFMPIFFAKIGLVVNIYTIKLI 390
           Q  GL IY   F +GF IPSGPPIGSTLV+RLDFIT W+FMP+FFA+IGL V+IY  KLI
Sbjct: 301 QALGLRIYFASFFIGFIIPSGPPIGSTLVDRLDFITTWIFMPVFFARIGLSVDIYNTKLI 360

Query: 391 NFVCMSFIVFVSALGKFLGALMISIYCKLPMRDAVSLGLILNSQGALELGIFKIKRREKL 450
           NF+CMSFIV VSALGKFLGALMIS+Y KLPMRDA+SLGLILNSQGALEL  F++K+R+K+
Sbjct: 361 NFICMSFIVIVSALGKFLGALMISMYYKLPMRDAISLGLILNSQGALELMTFRMKKRDKV 420

Query: 451 IDDEAFAVACICMLVIVAIITPIIRYLLDPSRRYIVHGRRTMMHSRPESDLCILVCIHDQ 510
           IDD+AF V C+ + V+VAIITP IRYLL PSRRYIV  RRT+MH+RPE DLC+LVCIHDQ
Sbjct: 421 IDDDAFVVGCLYITVMVAIITPAIRYLLHPSRRYIVKKRRTVMHTRPEHDLCVLVCIHDQ 480

Query: 511 EDVPSAISLLDAL-NPTRRSHLVVYMLHLVELLSRAQPKLIHHKLMEARALSSYSSEPIL 570
           EDVPSAI+LLDAL NP ++S LVVYMLHLVELL  AQPKLIHH+  + +   SYSSEPI+
Sbjct: 481 EDVPSAINLLDALNNPMKQSQLVVYMLHLVELLGHAQPKLIHHRFTKVKTSRSYSSEPIV 540

Query: 571 NAFKYFGEINSGIVAINSFTAISPFATIHDDVCSLALDKRSSLILVPFHKRFHSNGVMSS 630
           NAFKYFG+ N+ IV IN FTAISPF T+HDDVCSLALDK+S LI VPFHKRFHSNGVMSS
Sbjct: 541 NAFKYFGDSNNEIVVINPFTAISPFTTMHDDVCSLALDKKSFLIFVPFHKRFHSNGVMSS 600

Query: 631 SKHKIKTVNQHILEKAPCSVALVVERRFLKVSQTIKNNLNSFQIAIVFIGGPDDHEAMFI 690
           SK+K+K VN +ILE APCSVALVVER FLK+S++I+  L SFQIA+VFIGG DD EAMFI
Sbjct: 601 SKYKLKMVNDNILENAPCSVALVVERGFLKLSKSIETCLYSFQIAVVFIGGADDREAMFI 660

Query: 691 GARMVGHPNINLTMIRLLENENVQNNDIKERRLDDEAVAEFRQTVGDNYRVRYIEEVVND 750
           GARM GH NINLTMIR+LE+E V +++ +E R+DDEAV EFR+   DNYRVRYIEEVV D
Sbjct: 661 GARMAGHANINLTMIRVLESEKVGSDEAEESRVDDEAVDEFRRMTVDNYRVRYIEEVVKD 720

Query: 751 GIGTICILRSMGSNFDLVMVGRRHSPYLALVQGLVLWNECTELGAIGEVLASSNFMGDAM 810
           GIGTICILRSMGSNFDLVMVGRRHSP  ALVQGLVLWNE TELGAIGEVLA+S+FMG+AM
Sbjct: 721 GIGTICILRSMGSNFDLVMVGRRHSPCSALVQGLVLWNEHTELGAIGEVLATSDFMGNAM 780

Query: 811 ILVVQQHTRVANEDQENHKETII 832
           ILV+QQHTRVANE+ +N +ETI+
Sbjct: 781 ILVIQQHTRVANENHDNAQETIL 802

BLAST of Lsi09G012350 vs. ExPASy TrEMBL
Match: A0A6J1F2I8 (cation/H(+) antiporter 15-like OS=Cucurbita moschata OX=3662 GN=LOC111441553 PE=4 SV=1)

HSP 1 Score: 1116.7 bits (2887), Expect = 0.0e+00
Identity = 584/806 (72.46%), Postives = 674/806 (83.62%), Query Frame = 0

Query: 31  MGSIIMEPDDIAAYISDGI-SNTSKNFTTICTFANRIHYNNDVFSGENPLDFSVPLLFLQ 90
           MGSIIMEPDD+AAYIS G   NT KN T ICTFANRIH +  VFSG NPL+FSVPLLFLQ
Sbjct: 1   MGSIIMEPDDVAAYISGGHGQNTLKNLTRICTFANRIH-STGVFSGVNPLEFSVPLLFLQ 60

Query: 91  LGICSGTIILFSRFLKPLGQPLIVSQILGGLILGFSGLSHLEKFRETIFPLRGFIFLDVV 150
           LG+ +GTIILFS  LKPLGQPLIVSQILGG++LG SGL HL +FRE IFPLRGF+ LDV+
Sbjct: 61  LGLSAGTIILFSMLLKPLGQPLIVSQILGGVVLGSSGLGHLGRFREVIFPLRGFVVLDVI 120

Query: 151 SALGHIFYFFMIGVQTDISMVKKIDKKTFSIGSCSAILALILITIYSIIFPNFVDIQTFN 210
           SALGH+FYFF+IGVQTDIS VKKIDKK F+IGSCS IL++IL TIYSI   N VDIQT  
Sbjct: 121 SALGHVFYFFLIGVQTDISFVKKIDKKAFAIGSCSVILSMILSTIYSITLVNIVDIQTVK 180

Query: 211 YLIALGRLETFINFPMVSSILHELHLINSEFGRMALSTSMASSIFGMGFSFLGGIFAPTS 270
           YL A+G  E+FINFPMV+S++ ELHLINSEFGR ALST+MAS+ F +  + LG + AP S
Sbjct: 181 YLFAIGGTESFINFPMVASLVSELHLINSEFGRFALSTAMASNFFSICLALLGALLAPQS 240

Query: 271 KARHATLSKTLTIMVLTVFVIFIFRHVTLWMIEKNPIGQPLKESFVITLLLGVLMIAFCS 330
           + ++ T+S     ++L   + F  R   +WMI+KNPIGQPLKE FV+TLLL VL++AFC 
Sbjct: 241 EGKYQTISTLYASLMLVAVIFFAIRPTIVWMIKKNPIGQPLKECFVVTLLLLVLVVAFCC 300

Query: 331 QVFGLPIYIGPFILGFTIPSGPPIGSTLVERLDFITCWVFMPIFFAKIGLVVNIYTIKLI 390
           Q  GL IYIGP  LG TIPSGPPIGS LV+RLDFIT WVFMPIFF KIGLVVNIY  KLI
Sbjct: 301 QASGLHIYIGPLFLGVTIPSGPPIGSALVDRLDFITSWVFMPIFFVKIGLVVNIYATKLI 360

Query: 391 NFVCMSFIVFVSALGKFLGALMISIYCKLPMRDAVSLGLILNSQGALELGIFKIKRREKL 450
           NF+CMSFIVFVSA+GKFLGAL+IS Y KL MRDAVSLGLILN QGALELG+FKI R+EKL
Sbjct: 361 NFLCMSFIVFVSAMGKFLGALLISTYFKLSMRDAVSLGLILNCQGALELGVFKIMRKEKL 420

Query: 451 IDDEAFAVACICMLVIVAIITPIIRYLLDPSRRYIVHGRRTMMHSRPESDLCILVCIHDQ 510
           I+DEA  V C+ ++V+VAIITPIIR+LLDPSRR+IVH RRT+MHSRPE DLC LVCIHDQ
Sbjct: 421 INDEALVVTCVWVMVVVAIITPIIRHLLDPSRRFIVHKRRTVMHSRPEFDLCALVCIHDQ 480

Query: 511 EDVPSAISLLDALNPTRRSHLVVYMLHLVELLSRAQPKLIHHKLMEARALSSYSSEPILN 570
           EDVPSAI+LLDALNPTRRSHLVVY+LHLVELL RA  +LI+HK ME R   S SS PI+N
Sbjct: 481 EDVPSAINLLDALNPTRRSHLVVYILHLVELLGRAHLELIYHKQMEVRTSRSCSSIPIVN 540

Query: 571 AFKYFGEINSGIVAINSFTAISPFATIHDDVCSLALDKRSSLILVPFHKRFHSNGVMSSS 630
           AFKYFGE  S I+AI  FTAISP +T+HD VCSLALDK++SLILVPFH+RFHSNGV+S S
Sbjct: 541 AFKYFGESKSEILAIYPFTAISPSSTMHDVVCSLALDKKTSLILVPFHQRFHSNGVLSLS 600

Query: 631 KHKIKTVNQHILEKAPCSVALVVERRFLKVSQTIKNNLNSFQIAIVFIGGPDDHEAMFIG 690
           ++K K VN HILE APCSVALVVER  LK  ++I  N + FQIA+VFIGGPDD EAMFIG
Sbjct: 601 QYKTKMVNHHILENAPCSVALVVERGILKTPKSIATNFHPFQIAMVFIGGPDDREAMFIG 660

Query: 691 ARMVGHPNINLTMIRLLENENVQNNDIKERRLDDEAVAEFRQTVGDNYRVRYIEEVVNDG 750
           ARMVGHPNINLT IRL E+ NV ++D+KERRLD+E+V EFRQ++ DN RV+YIEE+V DG
Sbjct: 661 ARMVGHPNINLTFIRLQESGNVPSSDVKERRLDNESVNEFRQSIADNNRVKYIEEMVKDG 720

Query: 751 IGTICILRSMGSNFDLVMVGRRHSPYLALVQGLVLWNECTELGAIGEVLASSNFMGDAMI 810
           IGTI ILRS+GS+FDLV+VGR+H+P LALVQGLVLWNE TELGAIGEVLASS+F+G+A I
Sbjct: 721 IGTISILRSLGSDFDLVIVGRQHNPCLALVQGLVLWNEQTELGAIGEVLASSDFIGNATI 780

Query: 811 LVVQQHTRVANEDQENHKETIISMDN 836
           LVVQQH RV NED+E ++E II MDN
Sbjct: 781 LVVQQHRRVVNEDEELNQEDIIPMDN 805

BLAST of Lsi09G012350 vs. ExPASy TrEMBL
Match: A0A5A7V313 (Cation/H(+) antiporter 15-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1661G00120 PE=4 SV=1)

HSP 1 Score: 1114.0 bits (2880), Expect = 0.0e+00
Identity = 572/803 (71.23%), Postives = 678/803 (84.43%), Query Frame = 0

Query: 31  MGSIIMEPDDIAAYISDGISN-TSKNFTTICTFANRIHYNNDVFSGENPLDFSVPLLFLQ 90
           MGSI+MEP+DIA YI+ GI +  +KNFTTICTFAN IH  + +F G NPL+FSVPLLFLQ
Sbjct: 1   MGSIVMEPNDIATYINGGIGHRPTKNFTTICTFANHIHCTS-IFIGANPLEFSVPLLFLQ 60

Query: 91  LGICSGTIILFSRFLKPLGQPLIVSQILGGLILGFSGLSHLEKFRETIFPLRGFIFLDVV 150
           LGICSGTIILFS+ LKPLGQPLIVSQILGGL+LG +GLS++E F+ETIFPLRGF+ LDVV
Sbjct: 61  LGICSGTIILFSQLLKPLGQPLIVSQILGGLVLGSTGLSNVEIFKETIFPLRGFVSLDVV 120

Query: 151 SALGHIFYFFMIGVQTDISMVKKIDKKTFSIGSCSAILALILITIYSIIFPNFVDIQTFN 210
           SALGHI Y+F+IGVQTDIS+VK ID KTF IGSC+ ILA +L+ IYS+   + +D+  F 
Sbjct: 121 SALGHIVYYFLIGVQTDISIVKNIDMKTFGIGSCATILATLLVIIYSMSLASIIDMTKFK 180

Query: 211 YLIALGRLETFINFPMVSSILHELHLINSEFGRMALSTSMASSIFGMGFSFLGGIFAPTS 270
           Y+  LG+LE+FINFPMV+S+++ELHL+NSEFGR++L++SMASS+  +  + LG I +P  
Sbjct: 181 YIFELGKLESFINFPMVASLVYELHLVNSEFGRISLTSSMASSLLSVSLTLLGSILSPNG 240

Query: 271 KARHATLSKTLTIMVLTVFVIFIFRHVTLWMIEKNPIGQPLKESFVITLLLGVLMIAFCS 330
             R   LS+T  IMVL + ++F  R  TLWM++ NPIGQPLKE FVITLLLGVL++AFC 
Sbjct: 241 TTRQQILSETFAIMVLILVIVFSIRPATLWMVKMNPIGQPLKECFVITLLLGVLVVAFCC 300

Query: 331 QVFGLPIYIGPFILGFTIPSGPPIGSTLVERLDFITCWVFMPIFFAKIGLVVNIYTIKLI 390
           Q  GL IY   F +GF IPSGPPIGSTLV+RLDFIT W+FMP+FFA+IGL V+IY  KLI
Sbjct: 301 QALGLRIYFASFFIGFIIPSGPPIGSTLVDRLDFITTWIFMPVFFARIGLSVDIYNTKLI 360

Query: 391 NFVCMSFIVFVSALGKFLGALMISIYCKLPMRDAVSLGLILNSQGALELGIFKIKRREKL 450
           NF+CMSFIV VSALGKFLGALMIS+Y KLPMRDA+SLGLILNSQGALEL  F++K+R+K+
Sbjct: 361 NFICMSFIVIVSALGKFLGALMISMYYKLPMRDAISLGLILNSQGALELMTFRMKKRDKV 420

Query: 451 IDDEAFAVACICMLVIVAIITPIIRYLLDPSRRYIVHGRRTMMHSRPESDLCILVCIHDQ 510
           IDD+AF V C+ + V+VAIITP IRYLL PSRRYIV  RRT+MH+RPE DLC+LVCIHDQ
Sbjct: 421 IDDDAFVVGCLYITVMVAIITPAIRYLLHPSRRYIVKKRRTVMHTRPEHDLCVLVCIHDQ 480

Query: 511 EDVPSAISLLDAL-NPTRRSHLVVYMLHLVELLSRAQPKLIHHKLMEARALSSYSSEPIL 570
           EDVPSAI+LLDAL NP ++S LVVYMLHLVELL  AQPKLIHH+  + +   SYSSEPI+
Sbjct: 481 EDVPSAINLLDALNNPMKQSQLVVYMLHLVELLGHAQPKLIHHRFTKVKTSRSYSSEPIV 540

Query: 571 NAFKYFGEINSGIVAINSFTAISPFATIHDDVCSLALDKRSSLILVPFHKRFHSNGVMSS 630
           NAFKYFG+ N+ IV IN FTAISPF T+HDDVCSLALDK+S LI VPFHKRFHSNGVMSS
Sbjct: 541 NAFKYFGDSNNEIVVINPFTAISPFTTMHDDVCSLALDKKSFLIFVPFHKRFHSNGVMSS 600

Query: 631 SKHKIKTVNQHILEKAPCSVALVVERRFLKVSQTIKNNLNSFQIAIVFIGGPDDHEAMFI 690
           SK+K+K VN +ILE APCSVALVVER FLK+S++I+  L SFQIA+VFIGG DD EAMFI
Sbjct: 601 SKYKLKMVNDNILENAPCSVALVVERGFLKLSKSIETCLYSFQIAVVFIGGADDREAMFI 660

Query: 691 GARMVGHPNINLTMIRLLENENVQNNDIKERRLDDEAVAEFRQTVGDNYRVRYIEEVVND 750
           GARM GH NI LTMIR+LE+E V + +++E R+DDEAV EFR+   DNYRVRYIEEVV D
Sbjct: 661 GARMAGHANITLTMIRVLESEKVGSGEVEESRVDDEAVDEFRRMTVDNYRVRYIEEVVKD 720

Query: 751 GIGTICILRSMGSNFDLVMVGRRHSPYLALVQGLVLWNECTELGAIGEVLASSNFMGDAM 810
           GIGTICILRSMGS+FDLVMVGRRHSP  ALVQGLVLWNE TELGAIGEVLA+S+FMG+AM
Sbjct: 721 GIGTICILRSMGSDFDLVMVGRRHSPCSALVQGLVLWNEHTELGAIGEVLATSDFMGNAM 780

Query: 811 ILVVQQHTRVANEDQENHKETII 832
           ILV+QQHTRVANE+ +N +ETI+
Sbjct: 781 ILVIQQHTRVANENHDNAQETIL 802

BLAST of Lsi09G012350 vs. ExPASy TrEMBL
Match: A0A6J1IZN7 (cation/H(+) antiporter 15-like OS=Cucurbita maxima OX=3661 GN=LOC111482115 PE=4 SV=1)

HSP 1 Score: 1102.0 bits (2849), Expect = 0.0e+00
Identity = 576/806 (71.46%), Postives = 668/806 (82.88%), Query Frame = 0

Query: 31  MGSIIMEPDDIAAYISDGIS-NTSKNFTTICTFANRIHYNNDVFSGENPLDFSVPLLFLQ 90
           MGSIIMEPDD+AAYIS   S NT KN T ICTFA+RIH +  VFSG NPL+FSVPLLFLQ
Sbjct: 1   MGSIIMEPDDVAAYISGCDSHNTLKNITRICTFADRIH-STGVFSGVNPLEFSVPLLFLQ 60

Query: 91  LGICSGTIILFSRFLKPLGQPLIVSQILGGLILGFSGLSHLEKFRETIFPLRGFIFLDVV 150
           LG+ +GTIILFS  LKPLGQPLIVSQILGG++LG SGL HL +FRE IFPLRGF+ LDV+
Sbjct: 61  LGLSAGTIILFSMLLKPLGQPLIVSQILGGVVLGSSGLGHLGRFREVIFPLRGFVVLDVI 120

Query: 151 SALGHIFYFFMIGVQTDISMVKKIDKKTFSIGSCSAILALILITIYSIIFPNFVDIQTFN 210
           SALGH+F+FF+IGVQTDIS VKKIDKK F+IGSCS IL++IL TIYSI   N VDIQT  
Sbjct: 121 SALGHVFFFFLIGVQTDISFVKKIDKKAFAIGSCSVILSMILSTIYSITLVNIVDIQTVK 180

Query: 211 YLIALGRLETFINFPMVSSILHELHLINSEFGRMALSTSMASSIFGMGFSFLGGIFAPTS 270
           YL A+G  E+FINFPMV+S++ ELHLINSEFGR ALST+MAS+ F +  + LG +  P  
Sbjct: 181 YLFAIGGTESFINFPMVASLVSELHLINSEFGRFALSTAMASNFFSICLALLGALLTPQD 240

Query: 271 KARHATLSKTLTIMVLTVFVIFIFRHVTLWMIEKNPIGQPLKESFVITLLLGVLMIAFCS 330
           + ++ T+S     ++L   + F  R   +WMI+KNPIGQPLKE FV+TLLL VL++AFC 
Sbjct: 241 EGKYQTISTLYASLMLVAVIFFAIRPTIVWMIKKNPIGQPLKECFVVTLLLLVLVVAFCC 300

Query: 331 QVFGLPIYIGPFILGFTIPSGPPIGSTLVERLDFITCWVFMPIFFAKIGLVVNIYTIKLI 390
           Q  GL IY GP  LG TIPSGPPIGS LV+RLDFIT WVFMPIFF KIGL VNIY IKLI
Sbjct: 301 QALGLHIYTGPLFLGVTIPSGPPIGSALVDRLDFITSWVFMPIFFVKIGLAVNIYAIKLI 360

Query: 391 NFVCMSFIVFVSALGKFLGALMISIYCKLPMRDAVSLGLILNSQGALELGIFKIKRREKL 450
           NF+CMSFIVFV+ALGKFLGAL+IS Y KL MRDAVSLGLILN QGALELG+FKI R+EKL
Sbjct: 361 NFLCMSFIVFVNALGKFLGALLISTYFKLSMRDAVSLGLILNCQGALELGVFKIMRKEKL 420

Query: 451 IDDEAFAVACICMLVIVAIITPIIRYLLDPSRRYIVHGRRTMMHSRPESDLCILVCIHDQ 510
           I+DEA  V C+ ++V+VAII+PIIR+LLDPSRR+IVH RRT+MHSRPE DLC+LVCIHDQ
Sbjct: 421 INDEALVVMCVWVMVVVAIISPIIRHLLDPSRRFIVHKRRTVMHSRPEFDLCVLVCIHDQ 480

Query: 511 EDVPSAISLLDALNPTRRSHLVVYMLHLVELLSRAQPKLIHHKLMEARALSSYSSEPILN 570
           EDVPSAI+LLDALNPTRRSHLVVY+LHLVELL RA  +LI+HK ME R   S SS PI+N
Sbjct: 481 EDVPSAINLLDALNPTRRSHLVVYILHLVELLGRAHLELIYHKQMEVRTSRSCSSIPIVN 540

Query: 571 AFKYFGEINSGIVAINSFTAISPFATIHDDVCSLALDKRSSLILVPFHKRFHSNGVMSSS 630
           AFKYFGE    I+AI  FTAISP  T+HD VCSLALDK++SLILVPFH+RFHSNGV+S S
Sbjct: 541 AFKYFGESKREILAIYPFTAISPSTTMHDVVCSLALDKKTSLILVPFHQRFHSNGVLSLS 600

Query: 631 KHKIKTVNQHILEKAPCSVALVVERRFLKVSQTIKNNLNSFQIAIVFIGGPDDHEAMFIG 690
           ++K K VN HILE APCSVALVVER  LK  ++I  N + FQI +VFIGGPDD EAMFIG
Sbjct: 601 QYKTKMVNHHILENAPCSVALVVERGILKTPKSIATNFHPFQIVMVFIGGPDDREAMFIG 660

Query: 691 ARMVGHPNINLTMIRLLENENVQNNDIKERRLDDEAVAEFRQTVGDNYRVRYIEEVVNDG 750
           ARMVGHPNINLT IRL E+ NV ++D+KERRLD+E+V EFRQ++ DN RV+YIEEVV DG
Sbjct: 661 ARMVGHPNINLTFIRLQESGNVPSSDVKERRLDNESVNEFRQSIADNNRVKYIEEVVKDG 720

Query: 751 IGTICILRSMGSNFDLVMVGRRHSPYLALVQGLVLWNECTELGAIGEVLASSNFMGDAMI 810
           IGTI ILRS+G +FDLV+VGR+H+P LALVQGLVLWNE TELGAIGEVLASS+F+G+A I
Sbjct: 721 IGTISILRSLGGDFDLVIVGRQHNPCLALVQGLVLWNEQTELGAIGEVLASSDFIGNATI 780

Query: 811 LVVQQHTRVANEDQENHKETIISMDN 836
           LVVQQH RV NED+E ++E II MDN
Sbjct: 781 LVVQQHRRVINEDEELNQEDIIPMDN 805

BLAST of Lsi09G012350 vs. ExPASy TrEMBL
Match: A0A5D3DF05 (Cation/H(+) antiporter 15-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold318G00110 PE=4 SV=1)

HSP 1 Score: 1097.4 bits (2837), Expect = 0.0e+00
Identity = 563/802 (70.20%), Postives = 670/802 (83.54%), Query Frame = 0

Query: 31  MGSIIMEPDDIAAYISDGIS-NTSKNFTTICTFANRIHYNNDVFSGENPLDFSVPLLFLQ 90
           MGSI+MEP+D+ AY++     N  KNF+TICTFANRIH +  VF+G NPL+FSV LL  Q
Sbjct: 1   MGSIVMEPEDVVAYMNGETGHNAFKNFSTICTFANRIHCSG-VFNGANPLEFSVSLLLFQ 60

Query: 91  LGICSGTIILFSRFLKPLGQPLIVSQILGGLILGFSGLSHLEKFRETIFPLRGFIFLDVV 150
           LGICSGTIILFS+ LKPLG PLIVSQILGGL+LG SGLSHLEKF+E +FPLRGF+ LDVV
Sbjct: 61  LGICSGTIILFSQLLKPLGLPLIVSQILGGLVLGSSGLSHLEKFKEKVFPLRGFVCLDVV 120

Query: 151 SALGHIFYFFMIGVQTDISMVKKIDKKTFSIGSCSAILALILITIYSIIFPNFVDIQTFN 210
           SALGHIFYFF+IG+QTDIS++K+ID K F IGSCS I A++LI+ YS+   N VD++   
Sbjct: 121 SALGHIFYFFLIGLQTDISILKEIDIKAFGIGSCSTIFAMLLISTYSLFLSNIVDVKKIQ 180

Query: 211 YLIALGRLETFINFPMVSSILHELHLINSEFGRMALSTSMASSIFGMGFSFLGGIFAPTS 270
           ++     LE+FI++PMV S+L+ELHLINS+FGR++LS SMASS+  +  + L  I +   
Sbjct: 181 HIFEFASLESFISYPMVVSLLYELHLINSKFGRISLSASMASSLLKICLTILAAIRSTNG 240

Query: 271 KARHATLSKTLTIMVLTVFVIFIFRHVTLWMIEKNPIGQPLKESFVITLLLGVLMIAFCS 330
           + +    +K  +I+VL + +I++ R  TLWM ++NPIGQPLKE FVITLLLGVL++AFC 
Sbjct: 241 ETKDLVSTKIFSIVVLILLIIYVIRPATLWMAKENPIGQPLKEYFVITLLLGVLVVAFCC 300

Query: 331 QVFGLPIYIGPFILGFTIPSGPPIGSTLVERLDFITCWVFMPIFFAKIGLVV-NIYTIKL 390
           Q FGL IY G F+LGF IPS PPIGSTLV+RL+FIT W+FMPIFF +IGLV+ NIYTI L
Sbjct: 301 QTFGLRIYFGSFLLGFIIPSDPPIGSTLVDRLEFITSWIFMPIFFVRIGLVIDNIYTITL 360

Query: 391 INFVCMSFIVFVSALGKFLGALMISIYCKLPMRDAVSLGLILNSQGALELGIFKIKRREK 450
           +N + MSFI+F+SALGKFLG+L+IS+Y K PMRDA+SLGLILNSQGALEL +FKI +++K
Sbjct: 361 VNLLSMSFIIFISALGKFLGSLIISMYYKFPMRDAISLGLILNSQGALELSMFKIMKKDK 420

Query: 451 LIDDEAFAVACICMLVIVAIITPIIRYLLDPSRRYIVHGRRTMMHSRPESDLCILVCIHD 510
           LIDDEAF V CI ++VIVAIITPIIRYLL PS+RYIVH RRT+MHSRPE DLC+LVCIHD
Sbjct: 421 LIDDEAFVVGCISIMVIVAIITPIIRYLLRPSKRYIVHKRRTVMHSRPEFDLCVLVCIHD 480

Query: 511 QEDVPSAISLLDALNPTRRSHLVVYMLHLVELLSRAQPKLIHHKLMEARALSSYSSEPIL 570
           QEDVPS I+LLDALNPTRRSHL+VYMLHLVELL RAQPKLIHHK    R+L S SS+PI+
Sbjct: 481 QEDVPSVINLLDALNPTRRSHLIVYMLHLVELLGRAQPKLIHHKHKMVRSLRSSSSKPIV 540

Query: 571 NAFKYFGEINSGIVAINSFTAISPFATIHDDVCSLALDKRSSLILVPFHKRFHSNGVMSS 630
           NAFKYF +  S IVAIN FTAISP  T+HDDVCSLALDK +SLIL PFHKR+HSNG++S 
Sbjct: 541 NAFKYFEDSKSNIVAINLFTAISPSTTMHDDVCSLALDKSTSLILAPFHKRYHSNGMVSF 600

Query: 631 SKHKIKTVNQHILEKAPCSVALVVERRFLKVSQTIKNNLNSFQIAIVFIGGPDDHEAMFI 690
           SKHK+K VN HIL+KAPCSV L+VER  L+VS++I+ NL SFQI ++FIGGPDD EAMFI
Sbjct: 601 SKHKLKIVNHHILDKAPCSVGLIVERGLLRVSKSIETNLQSFQIVVIFIGGPDDREAMFI 660

Query: 691 GARMVGHPNINLTMIRLLENENVQNNDIKERRLDDEAVAEFRQTVGDNYRVRYIEEVVND 750
           GARMVGH NINLTMIRLL+NENV  +D KE+RLDDEAVAEFRQ + +NYRVRY EEVV D
Sbjct: 661 GARMVGHVNINLTMIRLLDNENVPKDDFKEKRLDDEAVAEFRQIIANNYRVRYKEEVVKD 720

Query: 751 GIGTICILRSMGSNFDLVMVGRRHSPYLALVQGLVLWNECTELGAIGEVLASSNFMGDAM 810
           G  TI ILRSMGSNFDL+MVGRRHSP+L+ VQGLVLW+E TELGAIGEVLASS+FMG+A 
Sbjct: 721 GTETISILRSMGSNFDLIMVGRRHSPFLSPVQGLVLWDERTELGAIGEVLASSDFMGNAT 780

Query: 811 ILVVQQHTRVANEDQENHKETI 831
           ILVVQQHT+VANED EN  ETI
Sbjct: 781 ILVVQQHTKVANEDHENPPETI 801

BLAST of Lsi09G012350 vs. NCBI nr
Match: XP_038878281.1 (cation/H(+) antiporter 15-like [Benincasa hispida])

HSP 1 Score: 1220.7 bits (3157), Expect = 0.0e+00
Identity = 623/807 (77.20%), Postives = 701/807 (86.86%), Query Frame = 0

Query: 31  MGSIIMEPDDIAAYISDGISNTSKNFTTICTFANRIHYNNDVFSGENPLDFSVPLLFLQL 90
           MGSI+MEP+DIAAY +DGI + S NFT IC FANRIH NN VFSG  PL+FSVPLL LQL
Sbjct: 1   MGSIVMEPEDIAAYSNDGIGHGSNNFTKICIFANRIHCNNGVFSGAKPLEFSVPLLLLQL 60

Query: 91  GICSGTIILFSRFLKPLGQPLIVSQILGGLILGFSGLSHLEKFRETIFPLRGFIFLDVVS 150
           GICSG IILFS+ LKPLGQPLIVSQI+GGL++G SGL ++EKFRE +FPL GFI LDVVS
Sbjct: 61  GICSGIIILFSQLLKPLGQPLIVSQIMGGLVIGASGLCYMEKFREAVFPLSGFIVLDVVS 120

Query: 151 ALGHIFYFFMIGVQTDISMVKKIDKKTFSIGSCSAILALILITIYSIIFPNFVDIQTFNY 210
           A G +FYFF+IGVQTDIS VK IDKK F IGSCSA+LA IL+ IYS+   NF+DIQ+  Y
Sbjct: 121 AFGRVFYFFLIGVQTDISFVKNIDKKAFGIGSCSAVLATILVIIYSVFVANFLDIQSSKY 180

Query: 211 LIALGRLETFINFPMVSSILHELHLINSEFGRMALSTSMASSIFGMGFSFLGGIFAPTSK 270
           L  LG+LETFINFPMVSS+L+ELHLINSEFGRMALSTSMAS+I G+  S LGGIF P  +
Sbjct: 181 LFVLGQLETFINFPMVSSLLYELHLINSEFGRMALSTSMASNIVGICLSILGGIFDPNGE 240

Query: 271 ARHATLSKTLTIMVLTVFVIFIFRHVTLWMIEKNPIGQPLKESFVITLLLGVLMIAFCSQ 330
            R   LSK L I+VL +  IF+FRH +LWMI KNPIGQPLKE FVITLLLGVLM+AFC Q
Sbjct: 241 TRQEDLSKNLVIVVLILVFIFVFRHASLWMIRKNPIGQPLKECFVITLLLGVLMVAFCCQ 300

Query: 331 VFGLPIYIGPFILGFTIPSGPPIGSTLVERLDFITCWVFMPIFFAKIGLVVNIYTIKLIN 390
             GL IY+GPF+LGF IPSGPPIGSTLVERLDFIT WVFMPIFFAKIGL+VN YT+KLIN
Sbjct: 301 ALGLQIYVGPFLLGFIIPSGPPIGSTLVERLDFITSWVFMPIFFAKIGLIVNNYTMKLIN 360

Query: 391 FVCMSFIVFVSALGKFLGALMISIYCKLPMRDAVSLGLILNSQGALELGIFKIKRREKLI 450
           F+CM+ +VF+SALGKFLGALMISIY KLPM+DA+SLGLILNSQGALELG+ KIKRREK+I
Sbjct: 361 FLCMTLVVFISALGKFLGALMISIYYKLPMKDALSLGLILNSQGALELGMLKIKRREKMI 420

Query: 451 DDEAFAVACICMLVIVAIITPIIRYLLDPSRRYIVHGRRTMMHSRPESDLCILVCIHDQE 510
           D+EA  V CICMLVIV+IITP+IRYL  PSRRYIV+ +RT+MH R E DLC+LVCI+DQE
Sbjct: 421 DEEALVVGCICMLVIVSIITPVIRYLFHPSRRYIVYKKRTVMHLRQEFDLCVLVCIYDQE 480

Query: 511 DVPSAISLLDALNPTRRSHLVVYMLHLVELLSRAQPKLIHHKLMEARALSSYSSEPILNA 570
           DVPSAI+LLDALNPTRRSHLVVYMLHLVELL R+QPKLIHHK  +A  L S SSEPI+NA
Sbjct: 481 DVPSAINLLDALNPTRRSHLVVYMLHLVELLGRSQPKLIHHKHRKAMTLRSSSSEPIINA 540

Query: 571 FKYFGEINSGIVAINSFTAISPFATIHDDVCSLALDKRSSLILVPFHKRFHSNGVMSSSK 630
           FKYF E ++  VAINSFTA+SPF TIHDDVCSLALDK+SSLILVPFHKRFHSNGV+SSSK
Sbjct: 541 FKYFAESSNETVAINSFTAVSPFKTIHDDVCSLALDKKSSLILVPFHKRFHSNGVLSSSK 600

Query: 631 HKIKTVNQHILEKAPCSVALVVERRFLKVSQTIKNNLNSFQIAIVFIGGPDDHEAMFIGA 690
           HKIK VN HI EKAPCS+A+VVER F  VS++I N+L SF+IA+VF+GGPDD EAMFIGA
Sbjct: 601 HKIKMVNHHIFEKAPCSIAIVVERGFSNVSKSITNHLQSFKIAVVFVGGPDDREAMFIGA 660

Query: 691 RMVGHPNINLTMIRLLENENVQNNDIKERRLDDEAVAEFRQTVGDNYRVRYIEEVVNDGI 750
           RM GH NINLTMIRLLENENV   +I+ERRLDDEAV EFRQ + +N+RVRYIEEVV DG 
Sbjct: 661 RMAGHFNINLTMIRLLENENVPREEIEERRLDDEAVVEFRQIIANNHRVRYIEEVVKDGT 720

Query: 751 GTICILRSMGSNFDLVMVGRRHSPYLALVQGLVLWNECTELGAIGEVLASSNFMGDAMIL 810
           GTI +LRSMGSNFDLVMVGRRHSP LAL QGLVLWNECTELGAIGEVLASS+FMG+A+IL
Sbjct: 721 GTISMLRSMGSNFDLVMVGRRHSPCLALAQGLVLWNECTELGAIGEVLASSDFMGNAIIL 780

Query: 811 VVQQHTRVANEDQENHKETIISMDNIE 838
           VVQQHTRVANEDQENH+ETII MDNI+
Sbjct: 781 VVQQHTRVANEDQENHQETIIFMDNID 807

BLAST of Lsi09G012350 vs. NCBI nr
Match: XP_038906053.1 (cation/H(+) antiporter 15-like [Benincasa hispida])

HSP 1 Score: 1154.4 bits (2985), Expect = 0.0e+00
Identity = 594/806 (73.70%), Postives = 686/806 (85.11%), Query Frame = 0

Query: 31  MGSIIMEPDDIAAYISDGIS-NTSKNFTTICTFANRIHYNNDVFSGENPLDFSVPLLFLQ 90
           M SI++EP++IAAYI  GI  + +KNFTTICTFAN IHY + +F+G +PL+FS+PLLFLQ
Sbjct: 1   MSSIVLEPNEIAAYIDGGIGHDPTKNFTTICTFANHIHYTS-IFTGADPLEFSIPLLFLQ 60

Query: 91  LGICSGTIILFSRFLKPLGQPLIVSQILGGLILGFSGLSHLEKFRETIFPLRGFIFLDVV 150
           LGICSGTIILFS+ LKPLGQPLIVSQILGGL+LG SGLSH+EKF+E IFPLRGFI LDVV
Sbjct: 61  LGICSGTIILFSQLLKPLGQPLIVSQILGGLVLGSSGLSHMEKFKEKIFPLRGFICLDVV 120

Query: 151 SALGHIFYFFMIGVQTDISMVKKIDKKTFSIGSCSAILALILITIYSIIFPNFVDIQTFN 210
           SALG+IFY+F+IGVQTDIS++KKID K F IGSCS ILA+IL+TIYS+   + V+I+   
Sbjct: 121 SALGYIFYYFLIGVQTDISIIKKIDMKAFGIGSCSTILAMILVTIYSMSLASIVEIKKLQ 180

Query: 211 YLIALGRLETFINFPMVSSILHELHLINSEFGRMALSTSMASSIFGMGFSFLGGIFAPTS 270
           Y+  LGRLETFINFPMV+S+++ELHLINSEFGR++LSTSMAS++     + LG I +P  
Sbjct: 181 YIFELGRLETFINFPMVASLVYELHLINSEFGRISLSTSMASTLLSTCLTLLGNILSPHG 240

Query: 271 KARHATLSKTLTIMVLTVFVIFIFRHVTLWMIEKNPIGQPLKESFVITLLLGVLMIAFCS 330
           + RH  L +  +++VL + +IF  R  TLWM+++N IGQPLKE FV TLLLG L +AFC 
Sbjct: 241 ETRHQVLLEIFSMVVLILVIIFSIRLATLWMVKRNSIGQPLKECFVTTLLLGALAVAFCC 300

Query: 331 QVFGLPIYIGPFILGFTIPSGPPIGSTLVERLDFITCWVFMPIFFAKIGLVVNIYTIKLI 390
           Q FGL IY   F LGF IPSGPPIGSTLV+RLDFIT W+FMP+FFA+IGL VNIYT KLI
Sbjct: 301 QAFGLRIYFASFFLGFIIPSGPPIGSTLVDRLDFITSWIFMPVFFARIGLAVNIYTTKLI 360

Query: 391 NFVCMSFIVFVSALGKFLGALMISIYCKLPMRDAVSLGLILNSQGALELGIFKIKRREKL 450
           NF+C +FIVFV ALGKFLGA  IS+Y KLPMRDA+SLGLILNSQGA EL  FK K REK+
Sbjct: 361 NFLCTAFIVFVCALGKFLGAFTISMYYKLPMRDAISLGLILNSQGAFELITFKKKAREKV 420

Query: 451 IDDEAFAVACICMLVIVAIITPIIRYLLDPSRRYIVHGRRTMMHSRPESDLCILVCIHDQ 510
           I+++AF VACICMLVIVAIITPI+RYLL PSRRYIVH RRT+MHS+PE DLC+LVCIHDQ
Sbjct: 421 INEDAFVVACICMLVIVAIITPIVRYLLHPSRRYIVHKRRTVMHSKPEFDLCVLVCIHDQ 480

Query: 511 EDVPSAISLLDALNPTRRSHLVVYMLHLVELLSRAQPKLIHHKLMEARALSSYSSEPILN 570
           EDVPSAI+LLDALNPTRRSHLVVYMLHLVELL RAQPKLIHHKL + R   S SSEPI+N
Sbjct: 481 EDVPSAINLLDALNPTRRSHLVVYMLHLVELLGRAQPKLIHHKLTKVRTSKSCSSEPIVN 540

Query: 571 AFKYFGEINSGIVAINSFTAISPFATIHDDVCSLALDKRSSLILVPFHKRFHSNGVMSSS 630
           AFKYFG+ NS IV IN FTAISP AT+HDDVCSLALDK +SLI+VPFHKRFHSNG MSSS
Sbjct: 541 AFKYFGDRNSEIVVINPFTAISPSATMHDDVCSLALDKMTSLIIVPFHKRFHSNGKMSSS 600

Query: 631 KHKIKTVNQHILEKAPCSVALVVERRFLKVSQTIKNNLNSFQIAIVFIGGPDDHEAMFIG 690
           K+KIK VN +IL  APCSV LVVER FLKVS++I++NL SFQ+A+VFIGGPDD EAMFIG
Sbjct: 601 KYKIKMVNHNILNNAPCSVVLVVERGFLKVSKSIESNLYSFQVAVVFIGGPDDREAMFIG 660

Query: 691 ARMVGHPNINLTMIRLLENENVQNNDIKERRLDDEAVAEFRQTVGDNYRVRYIEEVVNDG 750
           ARM GH NINLTMIRLLE+ENV ++D+KER LDDEAV EFR+ +  NYRVRYIEEVV DG
Sbjct: 661 ARMAGHTNINLTMIRLLESENVPSDDVKERMLDDEAVVEFRKIIAGNYRVRYIEEVVKDG 720

Query: 751 IGTICILRSMGSNFDLVMVGRRHSPYLALVQGLVLWNECTELGAIGEVLASSNFMGDAMI 810
            GTICILRSMGSNFDLVMVGR+HSP  ALVQGLVLWNE TELGAIGEVLASS+FMG+AM+
Sbjct: 721 TGTICILRSMGSNFDLVMVGRQHSPRSALVQGLVLWNEHTELGAIGEVLASSDFMGNAMV 780

Query: 811 LVVQQHTRVANEDQENHKETIISMDN 836
           LVVQQHTRVANE QEN ++TI    N
Sbjct: 781 LVVQQHTRVANEGQENSQKTIFMDSN 805

BLAST of Lsi09G012350 vs. NCBI nr
Match: XP_038883080.1 (cation/H(+) antiporter 15-like [Benincasa hispida])

HSP 1 Score: 1130.2 bits (2922), Expect = 0.0e+00
Identity = 581/802 (72.44%), Postives = 684/802 (85.29%), Query Frame = 0

Query: 31  MGSIIMEPDDIAAYISDGISN-TSKNFTTICTFANRIHYNNDVFSGENPLDFSVPLLFLQ 90
           MGSI+MEP+DIA Y++ G  +  SKN TTICTFANR+H +  +F+G NPL+FS  LLFLQ
Sbjct: 1   MGSIVMEPNDIATYMNGGNGHKASKNITTICTFANRVH-STGIFTGVNPLEFSASLLFLQ 60

Query: 91  LGICSGTIILFSRFLKPLGQPLIVSQILGGLILGFSGLSHLEKFRETIFPLRGFIFLDVV 150
           LGICS TII FSR LKPLGQPLIVSQILGG+ILG SGLSHL+KF++TIFPLRGF+ LD+V
Sbjct: 61  LGICSVTIIFFSRLLKPLGQPLIVSQILGGIILGSSGLSHLDKFKQTIFPLRGFVCLDLV 120

Query: 151 SALGHIFYFFMIGVQTDISMVKKIDKKTFSIGSCSAILALILITIYSIIFPNFVDIQTFN 210
           SALGHIFYFF+IG+QTDIS VKKIDKK F IGS S ILA+IL+T+YSI+  N VDI+   
Sbjct: 121 SALGHIFYFFLIGLQTDISFVKKIDKKAFCIGSFSTILAMILVTVYSIVLANAVDIKKIK 180

Query: 211 YLIALGRLETFINFPMVSSILHELHLINSEFGRMALSTSMASSIFGMGFSFLGGIFAPTS 270
           Y+  LGRLE+FI+FPMV+S+LHELHLINSEFG+++LSTSMAS++  + F+ L  I +P  
Sbjct: 181 YIYELGRLESFISFPMVASLLHELHLINSEFGKISLSTSMASTLLSICFTLLRTIMSPRG 240

Query: 271 KARHATLSKTLTIMVLTVFVIFIFRHVTLWMIEKNPIGQPLKESFVITLLLGVLMIAFCS 330
           + +H  LS+   I+VL + ++F+ R  TLWM + NP GQPLKE FVITLLL VL +AFC 
Sbjct: 241 EVKHHVLSENFAIVVLILVIVFVIRPTTLWMAKVNPTGQPLKEYFVITLLLAVLTVAFCC 300

Query: 331 QVFGLPIYIGPFILGFTIPSGPPIGSTLVERLDFITCWVFMPIFFAKIGLVV-NIYTIKL 390
           Q FGL IY   F+LGFT+PS PP+GSTLVERLDFIT W+FMPIFF +IGLVV NIY+IKL
Sbjct: 301 QTFGLRIYFASFLLGFTMPSEPPVGSTLVERLDFITSWIFMPIFFVRIGLVVDNIYSIKL 360

Query: 391 INFVCMSFIVFVSALGKFLGALMISIYCKLPMRDAVSLGLILNSQGALELGIFKIKRREK 450
           +NF+ MSFIV +SALGK LGALMIS+Y +L MRDA+SLGLILNSQGALEL +FK+KRR+K
Sbjct: 361 MNFLYMSFIVIISALGKLLGALMISMYYRLHMRDAISLGLILNSQGALELSMFKVKRRDK 420

Query: 451 LIDDEAFAVACICMLVIVAIITPIIRYLLDPSRRYIVHGRRTMMHSRPESDLCILVCIHD 510
           +I+DEAF V CI ++V VAII P+IRYLL PS+RY VH RRT+M+S PE DL +LVCI+D
Sbjct: 421 VINDEAFVVGCISIMVTVAIIAPVIRYLLRPSKRYKVHKRRTVMNSGPEFDLSLLVCIYD 480

Query: 511 QEDVPSAISLLDALNPTRRSHLVVYMLHLVELLSRAQPKLIHHKLMEARALSSYSSEPIL 570
           QEDVPSAI+LLDALNPT+RSHL+VYMLHLVELL  AQPK IHHKL +AR L S SSEPI+
Sbjct: 481 QEDVPSAINLLDALNPTKRSHLIVYMLHLVELLGSAQPKFIHHKLNKARTLRSCSSEPIV 540

Query: 571 NAFKYFGEINSGIVAINSFTAISPFATIHDDVCSLALDKRSSLILVPFHKRFHSNGVMSS 630
           NAFKYFGE NS IV IN FT+ISPF T+HDDVCSLAL+K +SLILVPFHK+FHSNG+MSS
Sbjct: 541 NAFKYFGESNSEIVEINPFTSISPFTTMHDDVCSLALNKNTSLILVPFHKKFHSNGMMSS 600

Query: 631 SKHKIKTVNQHILEKAPCSVALVVERRFLKVSQTIKNNLNSFQIAIVFIGGPDDHEAMFI 690
           SKHKIKTVN HIL+KAPCSVAL+VER FLK  ++I+ NL+SFQIAIVF+GGPDD EAMFI
Sbjct: 601 SKHKIKTVNHHILDKAPCSVALIVERGFLKAPKSIETNLHSFQIAIVFVGGPDDREAMFI 660

Query: 691 GARMVGHPNINLTMIRLLENENVQNNDIKERRLDDEAVAEFRQTVGDNYRVRYIEEVVND 750
           GARMVGH N+NLTMIRLL+NENV N+D+KERRLDDEAVAEF Q + DNYRVRYIEEVV D
Sbjct: 661 GARMVGHTNVNLTMIRLLDNENVPNDDVKERRLDDEAVAEFHQIISDNYRVRYIEEVVTD 720

Query: 751 GIGTICILRSMGSNFDLVMVGRRHSPYLALVQGLVLWNECTELGAIGEVLASSNFMGDAM 810
           G  TI ILRSMGSNFDLVMVGRRHSP+ A +QGLVLWNE TELG IGEVLA+S+ +G+A+
Sbjct: 721 GTETISILRSMGSNFDLVMVGRRHSPFWASLQGLVLWNERTELGEIGEVLATSDLIGNAI 780

Query: 811 ILVVQQHTRVANEDQENHKETI 831
           ILVVQQHTRVANED+E+H ETI
Sbjct: 781 ILVVQQHTRVANEDEESHHETI 801

BLAST of Lsi09G012350 vs. NCBI nr
Match: XP_008450215.1 (PREDICTED: cation/H(+) antiporter 15-like [Cucumis melo])

HSP 1 Score: 1119.8 bits (2895), Expect = 0.0e+00
Identity = 575/803 (71.61%), Postives = 680/803 (84.68%), Query Frame = 0

Query: 31  MGSIIMEPDDIAAYISDGISN-TSKNFTTICTFANRIHYNNDVFSGENPLDFSVPLLFLQ 90
           MGSI+MEP+DIA YI+ GI +  +KNFTTICTFAN IH  + +F G NPL+FSVPLLFLQ
Sbjct: 1   MGSIVMEPNDIATYINGGIGHRPTKNFTTICTFANHIHCTS-IFIGANPLEFSVPLLFLQ 60

Query: 91  LGICSGTIILFSRFLKPLGQPLIVSQILGGLILGFSGLSHLEKFRETIFPLRGFIFLDVV 150
           LGICSGTIILFS+ LKPLGQPLIVSQILGGL+LG +GLS++E F+ETIFPLRGF+ LDVV
Sbjct: 61  LGICSGTIILFSQLLKPLGQPLIVSQILGGLVLGSTGLSNVEIFKETIFPLRGFVSLDVV 120

Query: 151 SALGHIFYFFMIGVQTDISMVKKIDKKTFSIGSCSAILALILITIYSIIFPNFVDIQTFN 210
           SALGHIFY+F+IGVQTDIS+VK ID KTF IGSC+ ILA +L+ IYS+   + +D+  F 
Sbjct: 121 SALGHIFYYFLIGVQTDISIVKNIDMKTFGIGSCATILATLLVIIYSMSLASIIDMTKFK 180

Query: 211 YLIALGRLETFINFPMVSSILHELHLINSEFGRMALSTSMASSIFGMGFSFLGGIFAPTS 270
           Y+  LG+LE+FINFPMV+S+++ELHL+NSEFGR++L++SMASS+  +  + LG I +P  
Sbjct: 181 YIFELGKLESFINFPMVASLVYELHLVNSEFGRISLTSSMASSLLSVSLTLLGSILSPNG 240

Query: 271 KARHATLSKTLTIMVLTVFVIFIFRHVTLWMIEKNPIGQPLKESFVITLLLGVLMIAFCS 330
             R   LS+T  IMVL + ++F  R  TLWM++ NPIGQPLKE FVITLLLGVL++AFC 
Sbjct: 241 TTRQQILSETFAIMVLILVIVFSIRPATLWMVKMNPIGQPLKECFVITLLLGVLVVAFCC 300

Query: 331 QVFGLPIYIGPFILGFTIPSGPPIGSTLVERLDFITCWVFMPIFFAKIGLVVNIYTIKLI 390
           Q  GL IY   F +GF IPSGPPIGSTLV+RLDFIT W+FMP+FFA+IGL V+IY  KLI
Sbjct: 301 QALGLRIYFASFFIGFIIPSGPPIGSTLVDRLDFITTWIFMPVFFARIGLSVDIYNTKLI 360

Query: 391 NFVCMSFIVFVSALGKFLGALMISIYCKLPMRDAVSLGLILNSQGALELGIFKIKRREKL 450
           NF+CMSFIV VSALGKFLGALMIS+Y KLPMRDA+SLGLILNSQGALEL  F++K+R+K+
Sbjct: 361 NFICMSFIVIVSALGKFLGALMISMYYKLPMRDAISLGLILNSQGALELMTFRMKKRDKV 420

Query: 451 IDDEAFAVACICMLVIVAIITPIIRYLLDPSRRYIVHGRRTMMHSRPESDLCILVCIHDQ 510
           IDD+AF V C+ + V+VAIITP IRYLL PSRRYIV  RRT+MH+RPE DLC+LVCIHDQ
Sbjct: 421 IDDDAFVVGCLYITVMVAIITPAIRYLLHPSRRYIVKKRRTVMHTRPEHDLCVLVCIHDQ 480

Query: 511 EDVPSAISLLDAL-NPTRRSHLVVYMLHLVELLSRAQPKLIHHKLMEARALSSYSSEPIL 570
           EDVPSAI+LLDAL NP ++S LVVYMLHLVELL  AQPKLIHH+  + +   SYSSEPI+
Sbjct: 481 EDVPSAINLLDALNNPMKQSQLVVYMLHLVELLGHAQPKLIHHRFTKVKTSRSYSSEPIV 540

Query: 571 NAFKYFGEINSGIVAINSFTAISPFATIHDDVCSLALDKRSSLILVPFHKRFHSNGVMSS 630
           NAFKYFG+ N+ IV IN FTAISPF T+HDDVCSLALDK+S LI VPFHKRFHSNGVMSS
Sbjct: 541 NAFKYFGDSNNEIVVINPFTAISPFTTMHDDVCSLALDKKSFLIFVPFHKRFHSNGVMSS 600

Query: 631 SKHKIKTVNQHILEKAPCSVALVVERRFLKVSQTIKNNLNSFQIAIVFIGGPDDHEAMFI 690
           SK+K+K VN +ILE APCSVALVVER FLK+S++I+  L SFQIA+VFIGG DD EAMFI
Sbjct: 601 SKYKLKMVNDNILENAPCSVALVVERGFLKLSKSIETCLYSFQIAVVFIGGADDREAMFI 660

Query: 691 GARMVGHPNINLTMIRLLENENVQNNDIKERRLDDEAVAEFRQTVGDNYRVRYIEEVVND 750
           GARM GH NINLTMIR+LE+E V +++ +E R+DDEAV EFR+   DNYRVRYIEEVV D
Sbjct: 661 GARMAGHANINLTMIRVLESEKVGSDEAEESRVDDEAVDEFRRMTVDNYRVRYIEEVVKD 720

Query: 751 GIGTICILRSMGSNFDLVMVGRRHSPYLALVQGLVLWNECTELGAIGEVLASSNFMGDAM 810
           GIGTICILRSMGSNFDLVMVGRRHSP  ALVQGLVLWNE TELGAIGEVLA+S+FMG+AM
Sbjct: 721 GIGTICILRSMGSNFDLVMVGRRHSPCSALVQGLVLWNEHTELGAIGEVLATSDFMGNAM 780

Query: 811 ILVVQQHTRVANEDQENHKETII 832
           ILV+QQHTRVANE+ +N +ETI+
Sbjct: 781 ILVIQQHTRVANENHDNAQETIL 802

BLAST of Lsi09G012350 vs. NCBI nr
Match: XP_022934362.1 (cation/H(+) antiporter 15-like [Cucurbita moschata])

HSP 1 Score: 1116.7 bits (2887), Expect = 0.0e+00
Identity = 584/806 (72.46%), Postives = 674/806 (83.62%), Query Frame = 0

Query: 31  MGSIIMEPDDIAAYISDGI-SNTSKNFTTICTFANRIHYNNDVFSGENPLDFSVPLLFLQ 90
           MGSIIMEPDD+AAYIS G   NT KN T ICTFANRIH +  VFSG NPL+FSVPLLFLQ
Sbjct: 1   MGSIIMEPDDVAAYISGGHGQNTLKNLTRICTFANRIH-STGVFSGVNPLEFSVPLLFLQ 60

Query: 91  LGICSGTIILFSRFLKPLGQPLIVSQILGGLILGFSGLSHLEKFRETIFPLRGFIFLDVV 150
           LG+ +GTIILFS  LKPLGQPLIVSQILGG++LG SGL HL +FRE IFPLRGF+ LDV+
Sbjct: 61  LGLSAGTIILFSMLLKPLGQPLIVSQILGGVVLGSSGLGHLGRFREVIFPLRGFVVLDVI 120

Query: 151 SALGHIFYFFMIGVQTDISMVKKIDKKTFSIGSCSAILALILITIYSIIFPNFVDIQTFN 210
           SALGH+FYFF+IGVQTDIS VKKIDKK F+IGSCS IL++IL TIYSI   N VDIQT  
Sbjct: 121 SALGHVFYFFLIGVQTDISFVKKIDKKAFAIGSCSVILSMILSTIYSITLVNIVDIQTVK 180

Query: 211 YLIALGRLETFINFPMVSSILHELHLINSEFGRMALSTSMASSIFGMGFSFLGGIFAPTS 270
           YL A+G  E+FINFPMV+S++ ELHLINSEFGR ALST+MAS+ F +  + LG + AP S
Sbjct: 181 YLFAIGGTESFINFPMVASLVSELHLINSEFGRFALSTAMASNFFSICLALLGALLAPQS 240

Query: 271 KARHATLSKTLTIMVLTVFVIFIFRHVTLWMIEKNPIGQPLKESFVITLLLGVLMIAFCS 330
           + ++ T+S     ++L   + F  R   +WMI+KNPIGQPLKE FV+TLLL VL++AFC 
Sbjct: 241 EGKYQTISTLYASLMLVAVIFFAIRPTIVWMIKKNPIGQPLKECFVVTLLLLVLVVAFCC 300

Query: 331 QVFGLPIYIGPFILGFTIPSGPPIGSTLVERLDFITCWVFMPIFFAKIGLVVNIYTIKLI 390
           Q  GL IYIGP  LG TIPSGPPIGS LV+RLDFIT WVFMPIFF KIGLVVNIY  KLI
Sbjct: 301 QASGLHIYIGPLFLGVTIPSGPPIGSALVDRLDFITSWVFMPIFFVKIGLVVNIYATKLI 360

Query: 391 NFVCMSFIVFVSALGKFLGALMISIYCKLPMRDAVSLGLILNSQGALELGIFKIKRREKL 450
           NF+CMSFIVFVSA+GKFLGAL+IS Y KL MRDAVSLGLILN QGALELG+FKI R+EKL
Sbjct: 361 NFLCMSFIVFVSAMGKFLGALLISTYFKLSMRDAVSLGLILNCQGALELGVFKIMRKEKL 420

Query: 451 IDDEAFAVACICMLVIVAIITPIIRYLLDPSRRYIVHGRRTMMHSRPESDLCILVCIHDQ 510
           I+DEA  V C+ ++V+VAIITPIIR+LLDPSRR+IVH RRT+MHSRPE DLC LVCIHDQ
Sbjct: 421 INDEALVVTCVWVMVVVAIITPIIRHLLDPSRRFIVHKRRTVMHSRPEFDLCALVCIHDQ 480

Query: 511 EDVPSAISLLDALNPTRRSHLVVYMLHLVELLSRAQPKLIHHKLMEARALSSYSSEPILN 570
           EDVPSAI+LLDALNPTRRSHLVVY+LHLVELL RA  +LI+HK ME R   S SS PI+N
Sbjct: 481 EDVPSAINLLDALNPTRRSHLVVYILHLVELLGRAHLELIYHKQMEVRTSRSCSSIPIVN 540

Query: 571 AFKYFGEINSGIVAINSFTAISPFATIHDDVCSLALDKRSSLILVPFHKRFHSNGVMSSS 630
           AFKYFGE  S I+AI  FTAISP +T+HD VCSLALDK++SLILVPFH+RFHSNGV+S S
Sbjct: 541 AFKYFGESKSEILAIYPFTAISPSSTMHDVVCSLALDKKTSLILVPFHQRFHSNGVLSLS 600

Query: 631 KHKIKTVNQHILEKAPCSVALVVERRFLKVSQTIKNNLNSFQIAIVFIGGPDDHEAMFIG 690
           ++K K VN HILE APCSVALVVER  LK  ++I  N + FQIA+VFIGGPDD EAMFIG
Sbjct: 601 QYKTKMVNHHILENAPCSVALVVERGILKTPKSIATNFHPFQIAMVFIGGPDDREAMFIG 660

Query: 691 ARMVGHPNINLTMIRLLENENVQNNDIKERRLDDEAVAEFRQTVGDNYRVRYIEEVVNDG 750
           ARMVGHPNINLT IRL E+ NV ++D+KERRLD+E+V EFRQ++ DN RV+YIEE+V DG
Sbjct: 661 ARMVGHPNINLTFIRLQESGNVPSSDVKERRLDNESVNEFRQSIADNNRVKYIEEMVKDG 720

Query: 751 IGTICILRSMGSNFDLVMVGRRHSPYLALVQGLVLWNECTELGAIGEVLASSNFMGDAMI 810
           IGTI ILRS+GS+FDLV+VGR+H+P LALVQGLVLWNE TELGAIGEVLASS+F+G+A I
Sbjct: 721 IGTISILRSLGSDFDLVIVGRQHNPCLALVQGLVLWNEQTELGAIGEVLASSDFIGNATI 780

Query: 811 LVVQQHTRVANEDQENHKETIISMDN 836
           LVVQQH RV NED+E ++E II MDN
Sbjct: 781 LVVQQHRRVVNEDEELNQEDIIPMDN 805

BLAST of Lsi09G012350 vs. TAIR 10
Match: AT2G13620.1 (cation/hydrogen exchanger 15 )

HSP 1 Score: 483.4 bits (1243), Expect = 3.7e-136
Identity = 285/805 (35.40%), Postives = 471/805 (58.51%), Query Frame = 0

Query: 58  TICTFANRIHYNNDVFSGENPLDFSVPLLFLQLGICSGTIILFSRFLKPLGQPLIVSQIL 117
           +I  +A  +   N V+ G+NPLDFS+PL  LQL +       F   LKP  QP ++S+IL
Sbjct: 12  SIICYAPSMITTNGVWQGDNPLDFSLPLFVLQLTLVVVVTRFFVFILKPFRQPRVISEIL 71

Query: 118 GGLILGFSGLSHLEKFRETIFPLRGFIFLDVVSALGHIFYFFMIGVQTDISMVKKIDKKT 177
           GG++LG S L    KF  TIFP R  + L+ ++ +G +++ F++GV+ DI +V+K  K+ 
Sbjct: 72  GGIVLGPSVLGRSTKFAHTIFPQRSVMVLETMANVGLLYFLFLVGVEMDIMVVRKTGKRA 131

Query: 178 FSIGSCSAILALILITIYSIIFPNFVD-IQTFNYLIALGRLETFINFPMVSSILHELHLI 237
            +I     +L  ++   +S       D +    Y++ LG   +   FP+++ IL EL LI
Sbjct: 132 LTIAIGGMVLPFLIGAAFSFSMHRSEDHLGQGTYILFLGVALSVTAFPVLARILAELKLI 191

Query: 238 NSEFGRMALSTSMASSIFGMGFSFLGGIFAPTSKARHATLSKTLTIMVLTVFVIFIFRHV 297
           N+E GR+++S ++ + +F      L    A + K   A+L   ++  V     +F+ R  
Sbjct: 192 NTEIGRISMSAALVNDMFAWILLALAIALAESDKTSFASLWVMISSAVFIAVCVFVVRPG 251

Query: 298 TLWMIEKNPIGQPLKESFVITLLLGVLMIAFCSQVFGLPIYIGPFILGFTIPSGPPIGST 357
             W+I K P G+   E  +  +L GV++  F +   G     G F+ G  IP+G P+G T
Sbjct: 252 IAWIIRKTPEGENFSEFHICLILTGVMISGFITDAIGTHSVFGAFVFGLVIPNG-PLGLT 311

Query: 358 LVERLDFITCWVFMPIFFAKIGLVVNIYTIK-LINFVCMSFIVFVSALGKFLGALMISIY 417
           L+E+L+     + +P+FFA  GL  NI  I+    ++ +  ++F++  GK +G ++++ +
Sbjct: 312 LIEKLEDFVSGLLLPLFFAISGLKTNIAAIQGPATWLTLFLVIFLACAGKVIGTVIVAFF 371

Query: 418 CKLPMRDAVSLGLILNSQGALELGIFKIKRREKLIDDEAFAVACICMLVIVAIITPIIRY 477
             +P+R+ ++LGL+LN++G +E+ +  + + +K++DDE FA   +  LV+  +ITPI+  
Sbjct: 372 HGMPVREGITLGLLLNTKGLVEMIVLNVGKDQKVLDDETFATMVLVALVMTGVITPIVTI 431

Query: 478 LLDPSRRYIVHGRRTMMHSRPESDLCILVCIHDQEDVPSAISLLDALNPTRRSHLVVYML 537
           L  P ++ + + RRT+  ++P+S+L +LVC+H   +VP+ I+LL+A +PT+RS + +Y+L
Sbjct: 432 LYKPVKKSVSYKRRTIQQTKPDSELRVLVCVHTPRNVPTIINLLEASHPTKRSPICIYVL 491

Query: 538 HLVELLSRAQPKLIHHKLME----ARALSSYSSEPILNAFKYFGEINSGIVAINSFTAIS 597
           HLVEL  RA   LI H   +    A   +   S+ I+NAF+ + E ++  VA+   TAIS
Sbjct: 492 HLVELTGRASAMLIVHNTRKSGRPALNRTQAQSDHIINAFENY-EQHAAFVAVQPLTAIS 551

Query: 598 PFATIHDDVCSLALDKRSSLILVPFHKRFHSNGVMSSSKHKIKTVNQHILEKAPCSVALV 657
           P++T+H+DVCSLA DKR S I++PFHK+   +G M S+    + VNQ++LE +PCSV ++
Sbjct: 552 PYSTMHEDVCSLAEDKRVSFIIIPFHKQQTVDGGMESTNPAYRLVNQNLLENSPCSVGIL 611

Query: 658 VERRFLKVSQTIKNNLNSFQIAIVFIGGPDDHEAMFIGARMVGHPNINLTMIRLLENEN- 717
           V+R  L  +  + +N  S Q+A++F GGPDD EA+    RM  HP I LT++R + +E+ 
Sbjct: 612 VDRG-LNGATRLNSNTVSLQVAVLFFGGPDDREALAYAWRMAQHPGITLTVLRFIHDEDE 671

Query: 718 --------VQNNDI--------KERRLDDEAVAEFRQTVGDNYRVRYIEEVVNDGIGTIC 777
                     ++D+        K+R+LDD+ +  FR    +   + YIE++V++G  T+ 
Sbjct: 672 ADTASTRATNDSDLKIPKMDHRKQRQLDDDYINLFRAENAEYESIVYIEKLVSNGEETVA 731

Query: 778 ILRSMGSNFDLVMVGRRHSPYLALVQGLVLWNECTELGAIGEVLASSNFMGDAMILVVQQ 837
            +RSM S+ DL +VGR       L  GL  W+EC ELGAIG++LASS+F     +LVVQQ
Sbjct: 732 AVRSMDSSHDLFIVGRGEGMSSPLTAGLTDWSECPELGAIGDLLASSDFAATVSVLVVQQ 791

Query: 838 HT-RVANEDQENHKETIISMDNIEV 839
           +    A ED  +  E+ +     +V
Sbjct: 792 YVGSWAQEDDMDFPESPVHSHETKV 813

BLAST of Lsi09G012350 vs. TAIR 10
Match: AT1G06970.1 (cation/hydrogen exchanger 14 )

HSP 1 Score: 397.1 bits (1019), Expect = 3.4e-110
Identity = 237/769 (30.82%), Postives = 425/769 (55.27%), Query Frame = 0

Query: 64  NRIHYNNDVFSGENPLDFSVPLLFLQLGICSGTIILFSRFLKPLGQPLIVSQILGGLILG 123
           N +  +  VF G +PL +++PL+ LQ+ +   T  L  R LKPL Q +I +Q+L G+ILG
Sbjct: 31  NHMLTSKGVFLGSDPLKYAMPLMLLQMSVIIITSRLLYRLLKPLKQGMISAQVLAGIILG 90

Query: 124 FSGLSHLEKFRETIFPLRGFIFLDVVSALGHIFYFFMIGVQTDISMVKKIDKKTFSIGSC 183
            S       + +   P+ G I L  +S LG   + F++G++ D S+++K   K   IG+ 
Sbjct: 91  PSLFGQSSAYMQMFLPISGKITLQTLSNLGFFIHLFLLGLRIDASIIRKAGSKAILIGTA 150

Query: 184 SAILALILITIYSIIFPNFVDI--QTFNYLIALGRLETFINFPMVSSILHELHLINSEFG 243
           S  L   L  +  +   N  ++     + +  +  L    +FP+ +++L EL+++NS+ G
Sbjct: 151 SYALPFSLGNLTVLFLKNTYNLPPDVVHCISTVISLNAMTSFPVTTTVLAELNILNSDLG 210

Query: 244 RMALSTSMASSIFGMGFSFLGGIFAPTSKARHATLSKTLTIMVLTVFVIFIF---RHVTL 303
           R+A + S+    F    + +  +F      R  TL+   + + +T  ++ IF   R   +
Sbjct: 211 RLATNCSIVCEAFSWIVALVFRMF-----LRDGTLASVWSFVWVTALILVIFFVCRPAII 270

Query: 304 WMIEKNPIG-QPLKESFVITLLLGVLMIAFCSQVFGLPIYIGPFILGFTIPSGPPIGSTL 363
           W+ E+  I      E     +++ +L I+  S+V G+    G F LG ++P GPP+G+ L
Sbjct: 271 WLTERRSISIDKAGEIPFFPIIMVLLTISLTSEVLGVHAAFGAFWLGVSLPDGPPLGTGL 330

Query: 364 VERLDFITCWVFMPIFFAKIGLVVNIYTIKLINFVCMSFIVFVSALGKFLGALMISIYCK 423
             +L+     + +P F +  GL  N + I   +   +  ++ ++   KFLG    S YC 
Sbjct: 331 TTKLEMFATSLMLPCFISISGLQTNFFIIGESHVKIIEAVILITYGCKFLGTAAASAYCN 390

Query: 424 LPMRDAVSLGLILNSQGALELGIFKIKRREKLIDDEAFAVACICMLVIVAIITPIIRYLL 483
           + + DA SL L++  QG +E+    + + EK+++ E F +  I +L++  I   ++  L 
Sbjct: 391 IQIGDAFSLALLMCCQGVIEIYTCVMWKDEKVLNTECFNLLIITLLLVTGISRFLVVCLY 450

Query: 484 DPSRRYIVHGRRTMMHSRPES-DLCILVCIHDQEDVPSAISLLDALNPTRRSHLVVYMLH 543
           DPS+RY    +RT++ +R  +    +L+C+++ E+VPS ++LL+A  P+R S + V+ LH
Sbjct: 451 DPSKRYRSKSKRTILDTRQRNLQFRLLLCVYNVENVPSMVNLLEASYPSRFSPISVFTLH 510

Query: 544 LVELLSRAQPKLIHHKLMEARALSSYSSEPILNAFKYFGEINSGIVAINSFTAISPFATI 603
           LVEL  RA   L+ H  M     ++  S  I+N F+ F + N G +    FTA +PF++I
Sbjct: 511 LVELKGRAHAVLVPHHQMNKLDPNTVQSTHIVNGFQRFEQQNQGTLMAQHFTAAAPFSSI 570

Query: 604 HDDVCSLALDKRSSLILVPFHKRFHSNGVMSSSKHKIKTVNQHILEKAPCSVALVVERRF 663
           +DD+C+LALDK+++LI++PFHK++  +G +      I+ +N ++LEKAPCSV + ++R  
Sbjct: 571 NDDICTLALDKKATLIVIPFHKQYAIDGTVDHVNPSIRNINLNVLEKAPCSVGIFIDRGE 630

Query: 664 LKVSQTIKNNLNSFQIAIVFIGGPDDHEAMFIGARMVGHPNINLTMIRLLENENVQNN-- 723
            +  +++  +     +A++FI G DD EA+    R+  HP +++TMI      ++Q N  
Sbjct: 631 TEGRRSVLMSYTWRNVAVIFIEGRDDAEALAFSMRIAEHPEVSVTMIHFRHKSSLQQNHV 690

Query: 724 -DIKERRLDDEAVAEFRQTVGDNYRVRYIEEVVNDGIGTICILRSMGSNFDLVMVGRRHS 783
            D++    +   + +F+       ++ Y EE+V DG+ T  ++ S+G +FDLV+VGR H 
Sbjct: 691 VDVESELAESYLINDFKNFAMSKPKISYREEIVRDGVETTQVISSLGDSFDLVVVGRDHD 750

Query: 784 PYLALVQGLVLWNECTELGAIGEVLASSNFMGDAMILVVQQHTRVANED 823
              +++ GL  W+EC ELG IG++ ASS+F    +++  Q+   +A ++
Sbjct: 751 LESSVLYGLTDWSECPELGVIGDMFASSDFHFSVLVIHQQEGDSLAMDN 794

BLAST of Lsi09G012350 vs. TAIR 10
Match: AT2G30240.1 (Cation/hydrogen exchanger family protein )

HSP 1 Score: 376.3 bits (965), Expect = 6.3e-104
Identity = 234/766 (30.55%), Postives = 423/766 (55.22%), Query Frame = 0

Query: 59  ICTFANRIHYNNDVFSGENPLDFSVPLLFLQLGICSGTIILFSRFLKPLGQPLIVSQILG 118
           +C   N +  +  +F   NPL +++PLL LQ+ +   T  L  R L+PL Q +I +Q+L 
Sbjct: 29  VCQAQNML-TSRGIFMKSNPLKYALPLLLLQMSVIIVTSRLIFRVLQPLKQGMISAQVLT 88

Query: 119 GLILGFSGLSHLEKFRETIFPLRGFIFLDVVSALGHIFYFFMIGVQTDISMVKKIDKKTF 178
           G++LG S L H   +     P  G I +  +S +G + + F++G++ D S+++K   K  
Sbjct: 89  GVVLGPSFLGHNVIYMNMFLPAGGKIIIQTLSNVGFVIHLFLLGLKIDGSIIRKAGSKAI 148

Query: 179 SIGSCSAILALIL--ITIYSIIFPNFVDIQTFNYLIALGRLETFINFPMVSSILHELHLI 238
            IG+ S      L  +TI  I     +     +   +   L +  +FP+ +++L EL+++
Sbjct: 149 LIGTASYAFPFSLGNLTIMFISKTMGLPSDVISCTSSAISLSSMTSFPVTTTVLAELNIL 208

Query: 239 NSEFGRMALSTSMASSIFGMGFSFLGGIFAPTSKARHATLSKTLTIMV-LTVFVIFIFRH 298
           NSE GR+A   SM   +      F+   F   ++ R  T    L++++ L + + F+FR 
Sbjct: 209 NSELGRLATHCSMVCEVCSW---FVALAFNLYTRDRTMTSLYALSMIIGLLLVIYFVFRP 268

Query: 299 VTLWMIEKNPIGQPLKE---SFVITLLLGVLMIAFCSQVFGLPIYIGPFILGFTIPSGPP 358
           + +W+ ++       K+    F + LLL +  ++   +  G+    G F LG ++P GPP
Sbjct: 269 IIVWLTQRKTKSMDKKDVVPFFPVLLLLSIASLS--GEAMGVHAAFGAFWLGVSLPDGPP 328

Query: 359 IGSTLVERLDFITCWVFMPIFFAKIGLVVNIYTIKLI---NFVCMSFIVFVSALGKFLGA 418
           +G+ L  +L+     +F+P F A  GL  N + I      + V +  I+ ++   KFLG 
Sbjct: 329 LGTELAAKLEMFASNLFLPCFIAISGLQTNFFEITESHEHHVVMIEIILLITYGCKFLGT 388

Query: 419 LMISIYCKLPMRDAVSLGLILNSQGALELGIFKIKRREKLIDDEAFAVACICMLVIVAII 478
              S YC+  + DA+ L  ++  QG +E+    + +  +++D E F +  I +L +  I 
Sbjct: 389 AAASAYCQTQIGDALCLAFLMCCQGIIEVYTTIVWKDAQVVDTECFNLVIITILFVTGIS 448

Query: 479 TPIIRYLLDPSRRYIVHGRRTMMHSRPES-DLCILVCIHDQEDVPSAISLLDALNPTRRS 538
             ++ YL DPS+RY    +RT++++R  +  L +L+ +++ E+VPS ++LL+A  PTR +
Sbjct: 449 RFLVVYLYDPSKRYKSKSKRTILNTRQHNLQLRLLLGLYNVENVPSMVNLLEATYPTRFN 508

Query: 539 HLVVYMLHLVELLSRAQPKLIHHKLMEARALSSYSSEPILNAFKYFGEINSGIVAINSFT 598
            +  + LHLVEL  RA   L  H  M     ++  S  I+NAF+ F +   G +    FT
Sbjct: 509 PISFFTLHLVELKGRAHALLTPHHQMNKLDPNTAQSTHIVNAFQRFEQKYQGALMAQHFT 568

Query: 599 AISPFATIHDDVCSLALDKRSSLILVPFHKRFHSNGVMSSSKHKIKTVNQHILEKAPCSV 658
           A +P+++I++D+C+LALDK+++LI++PFHK++  +G +      I+T+N ++L+ APCSV
Sbjct: 569 AAAPYSSINNDICTLALDKKATLIVIPFHKQYAIDGTVGQVNGPIRTINLNVLDAAPCSV 628

Query: 659 ALVVERRFLKVSQTIKNNLNSFQIAIVFIGGPDDHEAMFIGARMVGHPNINLTMIRLLEN 718
           A+ ++R   +  +++        +A++FIGG DD EA+ +  RM   P++N+TMI     
Sbjct: 629 AIFIDRGETEGRRSVLMTNTWQNVAMLFIGGKDDAEALALCMRMAEKPDLNVTMIHFRHK 688

Query: 719 ENVQNNDIKERRLDDEAVAEFRQTVGDNYRVRYIEEVVNDGIGTICILRSMGSNFDLVMV 778
             +Q+ D  +   +   +++F+    +  ++ Y+EE+V DG+ T  ++ S+G  +D+V+V
Sbjct: 689 SALQDEDYSDMS-EYNLISDFKSYAANKGKIHYVEEIVRDGVETTQVISSLGDAYDMVLV 748

Query: 779 GRRHSPYLALVQGLVLWNECTELGAIGEVLASSNFMGDAMILVVQQ 815
           GR H    +++ GL  W+EC ELG IG++L S +F    +++  QQ
Sbjct: 749 GRDHDLESSVLYGLTDWSECPELGVIGDMLTSPDFHFSVLVVHQQQ 787

BLAST of Lsi09G012350 vs. TAIR 10
Match: AT5G22900.1 (cation/H+ exchanger 3 )

HSP 1 Score: 368.2 bits (944), Expect = 1.7e-101
Identity = 244/758 (32.19%), Postives = 419/758 (55.28%), Query Frame = 0

Query: 103 FLKPLGQPLIVSQILGGLILGFSGLSH---------LEKFRETIFPLRGFIFLDVVSALG 162
           FL+ LG     S +L G++L  S L            E ++E +F L         +A  
Sbjct: 75  FLRRLGMIRFTSHMLTGVLLSKSFLKENSAARRFFSTEDYKEIVFSL--------TAACS 134

Query: 163 HIFYFFMIGVQTDISMVKKIDKKTFSIGSCSAILALILITIYSIIFPNFVDIQTFNYLIA 222
           ++ ++F++GV+ D  +++   +K  +IG  S +L+ ++ ++  I F N  D+ T N    
Sbjct: 135 YMMFWFLMGVKMDTGLIRTTGRKAITIGLSSVLLSTLVCSV--IFFGNLRDVGTKNSDHT 194

Query: 223 LGRLETFI--------NFPMVSSILHELHLINSEFGRMALSTSMAS----SIFGMGFSF- 282
           L  LE  +        +FP+V ++L EL L NSE GR+A+S+++ S    SI      F 
Sbjct: 195 LNSLEYVVIYSIQCLSSFPVVGNLLFELRLQNSELGRLAISSAVISDFSTSILASVLIFM 254

Query: 283 ---------LGGIFAPTSKARHATLSKTLTIMVLTVFVIFIFRHVTLWMIEKNPIGQPLK 342
                    LG +F     A +  L +   +++     I++FR +  ++I++ P G+P+K
Sbjct: 255 KELKDEQTRLGSVFIGDVIAGNRPLMRAGIVVLFVCIAIYVFRPLMFYIIKQTPSGRPVK 314

Query: 343 ESFVITLLLGVLMIAFCSQVFGLPIYIGPFILGFTIPSGPPIGSTLVERLDFITCWVFMP 402
             ++ T+++ V   A  +      I++GPFILG  +P GPP+GS ++++ +      F+P
Sbjct: 315 AIYLSTIIVMVSGSAILANWCKQSIFMGPFILGLAVPHGPPLGSAIIQKYESAIFGTFLP 374

Query: 403 IFFAKIGLVVNIYTI---KLINFVCMSFIVFVSALGKFLGALMISIYCKLPMRDAVSLGL 462
            F A     ++I  +   + +N + +  I+  S + KF+   + +++  +PM D  +L L
Sbjct: 375 FFIASSSTEIDISALFGWEGLNGIIL--IMVTSFVVKFIFTTVPALFYGMPMEDCFALSL 434

Query: 463 ILNSQGALELGIFKIKRREKLIDDEAFAVACICMLVIVAIITPIIRYLLDPSRRYIVHGR 522
           I++ +G  ELG + +  +   +  E F VAC+ + +  AII PI+RYL DPSR Y  + +
Sbjct: 435 IMSFKGIFELGAYALAYQRGSVRPETFTVACLYITLNSAIIPPILRYLYDPSRMYAGYEK 494

Query: 523 RTMMHSRPESDLCILVCIHDQEDVPSAISLLDALNPTRRSHLVVYMLHLVELLSRAQPKL 582
           R M H +P S+L IL CI+  +D+   I+LL+A+ P+R S +  Y+LHL+EL+ +A P  
Sbjct: 495 RNMQHLKPNSELRILSCIYRTDDISPMINLLEAICPSRESPVATYVLHLMELVGQANPIF 554

Query: 583 IHHKLMEARALSSYSSEPILNAFKYFGEINSGIVAINSFTAISPFATIHDDVCSLALDKR 642
           I HKL   R   +  S  +L +F+ F +   G V ++++TA+S   T+H D+C LAL+  
Sbjct: 555 ISHKLQTRRTEETSYSNNVLVSFEKFRKDFYGSVFVSTYTALSMPDTMHGDICMLALNNT 614

Query: 643 SSLILVPFHKRFHSNG-VMSSSKHKIKTVNQHILEKAPCSVALVVER------RFLKVSQ 702
           +SLIL+PFH+ + ++G  + S+ + I+ +N+ +L+ APCSV + V R            +
Sbjct: 615 TSLILLPFHQTWSADGSALISNNNMIRNLNKSVLDVAPCSVGVFVYRSSSGRKNISSGRK 674

Query: 703 TIKN---NLNSFQIAIVFIGGPDDHEAMFIGARMVGHPNINLTMIRLL-ENENVQNNDIK 762
           TI     NL+S+ I ++F+GG DD EA+ +  RM   P IN+T++RL+  +E  + N + 
Sbjct: 675 TINGTVPNLSSYNICMIFLGGKDDREAVTLATRMARDPRINITIVRLITTDEKARENTVW 734

Query: 763 ERRLDDEAVAEFR-QTVGDNYRVRYIEEVVNDGIGTICILRSMGSNFDLVMVGRRHSPYL 815
           ++ LDDE + + +  T+ D   + Y E+ + D   T  +LRSM S+FD+ +VGR +    
Sbjct: 735 DKMLDDELLRDVKSNTLVD---IFYSEKAIEDAAETSSLLRSMVSDFDMFIVGRGNGRTS 794

BLAST of Lsi09G012350 vs. TAIR 10
Match: AT3G17630.1 (cation/H+ exchanger 19 )

HSP 1 Score: 367.9 bits (943), Expect = 2.2e-101
Identity = 239/772 (30.96%), Postives = 414/772 (53.63%), Query Frame = 0

Query: 69  NNDVFSGENPLDFSVPLLFLQLGICSGTIILFSRFLKPLGQPLIVSQILGGLILGFSGLS 128
           +N  F  E+PLDF++PL+ LQ+ +      L + FLKPL QP ++++I+GG++LG S L 
Sbjct: 18  SNGAFQNESPLDFALPLIILQIVLVVVFTRLLAYFLKPLKQPRVIAEIIGGILLGPSALG 77

Query: 129 HLEKFRETIFPLRGFIFLDVVSALGHIFYFFMIGVQTDISMVKKIDKKTFSIGSCSAILA 188
             + + +TIFP +    LD ++ +G +F+ F++G++ D + +KK  KK+  I      L 
Sbjct: 78  RSKAYLDTIFPKKSLTVLDTLANIGLLFFLFLVGLELDFAAIKKTGKKSLLIAIAGISLP 137

Query: 189 LILITIYSIIFPNFVD--IQTFNYLIALGRLETFINFPMVSSILHELHLINSEFGRMALS 248
            I+    S +    +   +    +++ +G   +   FP+++ IL EL L+ ++ GRMA+S
Sbjct: 138 FIVGVGTSFVLSATISKGVDQLPFIVFMGVALSITAFPVLARILAELKLLTTDIGRMAMS 197

Query: 249 TSMASSIFGMGFSFLGGIFAPTSKARHATLSKTLTIMVLTVFVIF---IFRHVTLWMIEK 308
              A+ +  +    L  +    S    + L     ++  T FVIF     + +  +M  +
Sbjct: 198 ---AAGVNDVAAWILLALAIALSGDGTSPLVSVWVLLCGTGFVIFAVVAIKPLLAYMARR 257

Query: 309 NPIGQPLKESFVITLLLGVLMIAFCSQVFGLPIYIGPFILGFTIPSGPPIGSTLVERLDF 368
            P G+P+KE +V   L  VL  +F +   G+    G F++G   P   P    L E+++ 
Sbjct: 258 CPEGEPVKELYVCVTLTVVLAASFVTDTIGIHALFGAFVVGIVAPKEGPFCRILTEKIED 317

Query: 369 ITCWVFMPIFFAKIGLVVNIYTIK-LINFVCMSFIVFVSALGKFLGALMISIYCKLPMRD 428
           +   + +P++FA  GL  ++ TI+   ++  +  ++  +  GK +G +  S+ CK+P R+
Sbjct: 318 LVSGLLLPLYFAASGLKTDVTTIRGAQSWGLLVLVILTTCFGKIVGTVGSSMLCKVPFRE 377

Query: 429 AVSLGLILNSQGALELGIFKIKRREKLIDDEAFAVACICMLVIVAIITPIIRYLLDPSRR 488
           AV+LG ++N++G +EL +  I +  K+++D+AFA+  +  L    I TPI+  +  P+R+
Sbjct: 378 AVTLGFLMNTKGLVELIVLNIGKDRKVLNDQAFAILVLMALFTTFITTPIVMLIYKPARK 437

Query: 489 YIVHGRRTMMHSRPESDLCILVCIHDQEDVPSAISLLDALNPT-RRSHLVVYMLHLVELL 548
              +  RT+     +S+L IL C H   ++P+ I+L+++   T ++  L VY +HL+EL 
Sbjct: 438 GAPYKHRTIQRKDHDSELRILACFHSTRNIPTLINLIESSRGTGKKGRLCVYAMHLMELS 497

Query: 549 SRAQPKLIHHKL----MEARALSSYSSEPILNAFKYFGEINSGIVAINSFTAISPFATIH 608
            R+    + HK     +        S++ ++ AF+ +  + +  VA+   TAIS  ++IH
Sbjct: 498 ERSSAIAMVHKARNNGLPIWNKIERSTDQMVIAFEAYQHLRA--VAVRPMTAISGLSSIH 557

Query: 609 DDVCSLALDKRSSLILVPFHKRFHSNGVMSSSKHKIKTVNQHILEKAPCSVALVVERRFL 668
           +D+C+ A  KR ++IL+PFHK    +G M S  H+   VNQ +L++APCSV ++V+R   
Sbjct: 558 EDICTSAHQKRVAMILLPFHKHQRMDGAMESIGHRFHEVNQRVLQRAPCSVGILVDRGLG 617

Query: 669 KVSQTIKNNLNSFQIAIVFIGGPDDHEAMFIGARMVGHPNINLTM---------IRLLEN 728
             SQ + + + ++++ I F GG DD EA+  G +MV HP I LT+         ++  E 
Sbjct: 618 GTSQVVASEV-AYKVVIPFFGGLDDREALAYGMKMVEHPGITLTVYKFVAARGTLKRFEK 677

Query: 729 ENVQNNDIKERRLDDEAVAEFRQTVGDNYRVRYIEEVVNDGIGTICILRSMGSNFDLVMV 788
                 + KE+  D+E V E       N  + Y E VV      I  L+SM S  +L +V
Sbjct: 678 SEHDEKEKKEKETDEEFVRELMNDPRGNESLAYEERVVESKDDIIATLKSM-SKCNLFVV 737

Query: 789 GRRHSPYLALVQGLVLWNECTELGAIGEVLASSNFMGDAMILVVQQHTRVAN 821
           GR      A V  LV   +C ELG +G +L+SS F   A +LVVQ +   A+
Sbjct: 738 GRN-----AAVASLVKSTDCPELGPVGRLLSSSEFSTTASVLVVQGYDPAAD 777

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SIT55.1e-13535.40Cation/H(+) antiporter 15 OS=Arabidopsis thaliana OX=3702 GN=CHX15 PE=2 SV=1[more]
Q9LMJ14.8e-10930.82Cation/H(+) antiporter 14 OS=Arabidopsis thaliana OX=3702 GN=CHX14 PE=2 SV=1[more]
O229208.9e-10330.55Cation/H(+) symporter 13 OS=Arabidopsis thaliana OX=3702 GN=CHX13 PE=1 SV=1[more]
Q9FFB82.4e-10032.19Cation/H(+) antiporter 3 OS=Arabidopsis thaliana OX=3702 GN=CHX3 PE=2 SV=1[more]
Q9LUN43.1e-10030.96Cation/H(+) antiporter 19 OS=Arabidopsis thaliana OX=3702 GN=CHX19 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3BNS60.0e+0071.61cation/H(+) antiporter 15-like OS=Cucumis melo OX=3656 GN=LOC103491876 PE=4 SV=1[more]
A0A6J1F2I80.0e+0072.46cation/H(+) antiporter 15-like OS=Cucurbita moschata OX=3662 GN=LOC111441553 PE=... [more]
A0A5A7V3130.0e+0071.23Cation/H(+) antiporter 15-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A6J1IZN70.0e+0071.46cation/H(+) antiporter 15-like OS=Cucurbita maxima OX=3661 GN=LOC111482115 PE=4 ... [more]
A0A5D3DF050.0e+0070.20Cation/H(+) antiporter 15-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
Match NameE-valueIdentityDescription
XP_038878281.10.0e+0077.20cation/H(+) antiporter 15-like [Benincasa hispida][more]
XP_038906053.10.0e+0073.70cation/H(+) antiporter 15-like [Benincasa hispida][more]
XP_038883080.10.0e+0072.44cation/H(+) antiporter 15-like [Benincasa hispida][more]
XP_008450215.10.0e+0071.61PREDICTED: cation/H(+) antiporter 15-like [Cucumis melo][more]
XP_022934362.10.0e+0072.46cation/H(+) antiporter 15-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT2G13620.13.7e-13635.40cation/hydrogen exchanger 15 [more]
AT1G06970.13.4e-11030.82cation/hydrogen exchanger 14 [more]
AT2G30240.16.3e-10430.55Cation/hydrogen exchanger family protein [more]
AT5G22900.11.7e-10132.19cation/H+ exchanger 3 [more]
AT3G17630.12.2e-10130.96cation/H+ exchanger 19 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006153Cation/H+ exchangerPFAMPF00999Na_H_Exchangercoord: 96..474
e-value: 5.7E-30
score: 104.4
IPR038770Sodium/solute symporter superfamilyGENE3D1.20.1530.20coord: 85..483
e-value: 2.8E-51
score: 176.5
NoneNo IPR availablePANTHERPTHR32468CATION/H + ANTIPORTERcoord: 48..825
NoneNo IPR availablePANTHERPTHR32468:SF114CATION/H(+) ANTIPORTER 15-LIKE ISOFORM X1coord: 48..825

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi09G012350.1Lsi09G012350.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1902600 proton transmembrane transport
biological_process GO:0006812 cation transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0015299 solute:proton antiporter activity