Lsi09G010710 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi09G010710
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Cysteine protease
Location	chr09: 16634382 .. 16640599 (+)
RNA-Seq Expression	Lsi09G010710
Synteny	Lsi09G010710

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAAGGGGGAAAGATCTGAATTTAACATGCTCTTCTGAAAGTACAACTGATATTATAGATAGAACTCATCAATCTGTTGGCCCAGAGTTAGGGTCTAAAAATCACATATCCTCCAAAGCCTCCTTATGGTCAGGCTTCTTTACATCCACTTTCTCAATCTTTGAACATCACAAAGAGTCCTCTGTCAGTGAAAAGAAGGCATTTCATTCTCGACACAATGTCTGGGCAACTGTAAGAAAAGTTATGACTAGTGGCTCAATGAGGAGAATTCAGGAGCGCATAATGGGTTCTCGCAGAAGTGGTGTCTATAGCTCGGGTGGTGATATATGGCTTCTTGGTGTGTGCCATAAAATTTCCCAAGATCAGGCTCCTGATGATGCAGTAACTAGCACTGGTGCAGCAGGATATGAGCTAGATTTTTCATCTAGAATTCTGATGACCTATCGTAAAGGTTTCATGGAATATGCTCTCTTTTAGTTATAATTATATCATAAGCTTGCATATATTTCCTGGACAACTTGCGGCTAACTTATTTTTTCTCTCCTTTTATAGGTTTTAATGTTATTCAAGACTCAAAATACACCAGTGATGTTAATTGGGGTTGCATGCTAAGAAGCAGCCAAATGCTTGTTGCTCAGGTAGACAGATTCATACATGATTTATTTTGGTGAGGTCTATGCTTTTCTATCCGTTGCTGTTCTCAATCTCGTGGCGTCTTCTATTTTTATTTATTTATTTGTACTGACGTTACTTTGGTTATGAGGGTCTCCCAAAAGGTATTCTGTTATTTCCTTTTATTAATCTTCATATTATCTCCAGGCATTACTTTTTCATAGATTAGGAAGATCATGGAGAAAGACTTCACAGAAGGTATGGCTGTATGGTGATGATACTGAAAGATTTGTTTGACTTACTGATTTATTTCCCAAACAAAATTATCACTAAACCTAATTTATTTTAATTGGCAGCCATTGGATAAAGAATATGTTGAAATTTTGCACCTTTTTGGTGACTCGGAGACATCGCCATTTTCAATTCATAATCTTCTTCAGGCAGGAAGGGCCTATGACCTTGCTGCTGGGTCATGGGTGGGACCATATGCCATGTGTAGGTCATGGGAGACTCTAGTCCGCTTAAAGAGGGAGACCCCTAATCTCCAAGACCAGCAACTTCCAATGGCCATTTACATTGTTTCTGGAGATGAAGATGGAGAGAGAGGTGGTGCTCCAGTTTTATGCATTGATGATGCCTCAAGACATTGTTTTGAGTTTTCAAAAGGCCAACTTGATTGGAGCCCCATTCTTTTATTAGTTCCTTTGGTCCTTGGACTTGAAAAAATCAACCCAAGGTATGTTATAATTGTATTTCTTAATACTTTGGGCTTGACTCTCATTTTAATAACAGTGAAATAGGATTCTCGTTCAAGATGTAGTACTTTTAGAGGCTATAGTTTTTCCCTAATAATTGGTTTTGCATCTTCATGTTTTAGCAGTTTTATCGAAAATTTTAATCATTGATAAGAAGTACTAAAATTTGATCCTCTAGGTTTGGAAATGGTTTCTGTATCGAGCTCTTTTAAGTCTAGCATTGCTTCATGGTGGGAGGATTTGTGGGGAACGGTGGGTGAAGGTTTTTCCTATTATTAAATTGGATTTTGTGAAAAGATAGTTAGAAAATGAAATGTAAAGATGTTTTTTTTCTTTTTTTTGGAGGATTTGTTGCTTATGGATGAGGCTAAAACCCAACCTAAGAAGCACAAGTACGGATACTTTAGTTTGGTTAATGTGTCCGTGTTGTTGGACACATCTTAGACACTTGTTGGTGCAATAAATGTGTTAAACGCTAGTTGTATAAAGTCAAAATAGATCCATTTATTTGTTTGACTTGCATCAAATAGTTGTTAAGTATGCTATTATCATTGTTTATTTTTTAGGGGACACTAAGTATACATTTTTTTTTTATATTTTATATTTTATATATTTTTTATAATTATAATAATTATTTTTTGATAATATGTGCTTGAAGCACCCATCACCTTTTTGCTTTTGACTACTTGTATCTTTAAGCATTGGTCTCTTTTCATTTTCTTAATGAAAAGTCTCGTGTCCATTTCAGAAAAGCACCCATCACTTGTGACAAAAGGTTTGATGACATTTAGGCCGTTTGGTTTCATGGAATCAGATAGATAGGAAACAGGGTGGTAATGGGCACAGAGTATGCATTCCCTTGTTTGAAACTAGGTTTATAAAGAAGGGCATGGGACCTATCCCCAAGGATCTTTTGTCGACAGGAGGGTATTTCACATCCTCCAAGTTACGTGGGTTTTCTTCATTTCCTTTCATGTCTCTCTTTCTTCTTTTTTTTCCCTCTTCTTCCCTCCTCTTAAACATATGCTGATTGTGACGGGAAAATGTGGTCAAGTGAATTTCTTGCTGCTAAAGGGGTCAAACTTAGGGGTGGAGAACTTTTGGTTGCTTCTAGTTCTTGCTCCTGATGTTGAGGTAACTAGGAATGAATTAAGAGCTCGATACAATTGGGTTGCTCTTGCAATGAGCTCAATTGTCAATTTCTTCCATCCTTTGGTTGAGATCGCCACTAATATCAATACTGTTTGAGCCACTATCTATCTTCTTCTTGTGAAAAGACATGTAGTTCAAGAACGAGCAGTCGGAGGCACTCTGATTTTTTTCCTTTTCTTGTTAAGACCAATCTGAACAATATTAGCACTGGATTAAGAAGTGGAATGTTACATTTCCATGGGACACACCTTTGCCTGTTTGACTCTCCTATTTTCTTATTCTAAAACCATTTTGTCTCAATAAAATATATACAACGGGAGTGAAATTTAGTGGATACTGTTACTTTCCAAACTGCTCATTGCCTTTGGATCCTTGCTCATATTAACAAGAATTCTATTTGTGTTCAATTTATAAGCCATAATAACAAAAGTGCTGATGGTTATTCACTTCATGGCCCCCGGTCCTAGCACAACCATGACTTGTTTGATTTCCAAATACCGTTTACCAAGTCTTAGGAGGGATCTTGAAATCTTTATTTATGTTTTAATCTTTCTCTGATTATTAGATAACATGGGGTTCCTTCTTAAAACCAATTAGCAACGAGAGTAGCCCATCAATCTTATAAAGAATGTGAGGTCTCTTGATTTTTCTAATGTTGGATCCTTAACATGCTGTCTCAAGATGGTTCCTCTTTCGGGTTCACCATTCTTGATCAGATCTAATTTTTTTTTTTTTTTTTAATTGGACTGAATACATGTTTGGGCTTTATGAGCTACGATACCATATTAGATAACATAGAGTTTCATCTCAAAACCAATTAGCAATGAGAAGAGTAGCCCATCTATCTTATAAAAAGTGTGAGGCCCTTTGATTTTTCCAATGTGAGATCCTTAAAACTGATGAGATCAAGGTGTAATAGAATAACCCTCTAAGGACCTCTCAAGTAATCTGGGAAAGTTATATTAGGCCACAAATTTGTGGTGGGCTTTCAATATTTAGCATGAATTACTTTTCCCCAAAGAGCATCCTATTCATGATAATATCTCCATATCCATTAGGCCAATATAGCCCTGTTTTCTTCTTTCAAACTTCGAAGCCCAAGGCCAGAATTGGGAATGGATCTAGTGACTAATTCCCAATTAACAAGATGTGGCAGAGCTCCCCTTCCAAAGAAGCTTTCGGTATAGTTTTTCATCAGCGATCACCACTTTTGATGGAATTTTGAAGATAGACATATCATAAGTGGGGAGGTTTGGGAGAGTAGCTTGTAGAAGAGTAAATCAGTCCCTCTTAGAAGAGTGTCGATGACCCCAAGAGAAAAGTCTTCTTTCAATCTTCTCAAAGAATGATAGGAGTCCAGAAAGCATCCAGAAAGCCATAATACTGCTTACCAAATAAAGGAAGTCCAAGGTAAATATTTGGGCATTGACCATGTCTACAATTAAAATTGTTTGCTATGAGCTTAATCTCTTCATGGTCAATGTTAATCCCCATGATTTCCAATTTTTTATGATTAACATTCAATCCAGAAGCCTTCTCAAGAGTGTCAATGAGGCTAAAATGGTTCTCAGGTCAACATTAAAGTGGGTAATGGAGTAAACACATCATTCTGGGTTGATAGATGGTTGAATTCCAAGACATGGGCTGCTCTTTACCCTGGATTACTCCCTTTCCTTGCACAAAGATTCCTTTATCCAGGACACTAGTGATGATCATGGTGGCTGGAATTTGGTTTTGAGAAATCTTTTGGGTGAGTAATTGGACGAATGACTTGGCATCATGAATTTATTGTTTCTAAATCCCTGTTGGTTGACCTTGACGACAATGGGCAGTAACGGGGGTGTCTACCTGGAAGCAATCTGAACTGATTTTATTTGAAGAAAATAAAAAAATTTCTTTTGGAAGCTTAGCCACTATGCCGTAAATACCCTTGACAGAATACAAGGCCGTGCCCCATACATGGTTTTGTCCACACAATTTTGCATCATCTGTAAATCTGTAAAGCTGCCCAGAACCTCAAAATTACTTGTTCGTCAGCTGTCAGTTTGCCAATAGGTTTTTGGAAAAATTTGATTGCTGCATCAAATTGGCACCTTACAATTTTGAATGACATCAACAATTTACTTAACTACTCTCTAAATGGCCACCCATAACATGGAAAGGGAAAAGAGTTTTTTGGATGCATCTCATTCGGGCTTTTTGACAACTTGGAGGGGACGCAACAACTGCCTCTTTCGATATAAGGATGATTCCTATGAAGTATTTTGTAACATATTATTTTCCTTGTAATGTCTTGGTGTATATGCTAGATCATATTCTCTCGCCTCCCTTTTGACCAATTGGAGAGATCTTCTATAACTCCTCCTCTGGTTTGGGCTATGGCCTTTTTTGTAAATTTCATGCATCAATGAAATTTTTTTTAACATAAAAAAATCTTTTACTGATTGCTATAAAGATGTATTTATGTGTTTGCTAAACTATAGCAGCCTTGCTCGACTATCGTGGAGACCTACATGTTATATTTATAGTTTGACTAGTAGAAATTGAAGCTTAGAAATTTATGCTCAACATATTTATTCAACTTGTGAAGGTACATTCCATCATTGCGAACAACATTTACCTTTCCCCAAAGCCTAGGGATCTTGGGAGGGAAGCCTGGTGCTTCAACATACATAGTGGGCGTCCAAGATGAAAATGCCTTTTACCTTGATCCACATGAAGTTCAGCAGGTACATTCATTTCCTTTGGAGATCTCTTTCTTAGAATATCTAAATTATTCTTGCTTGTATGTGTCTGATATTTGATATCATCATGCTGATAATAATAGAGAGATGTGTGTTTCATTGGGGTTTCCATGAGAGTAGAAGAACGTAATTAAGCCCCCTTCAGTGAGGGTGAATTGAAAGAGGTTCATGTTGACAGTGAGTCAGGTTAGAAGGTGGAGATTGAATGACGTGCCTTTCTTCAGAAGATGACAAGCACCCGAGGCATTGTGTTGGGTACAGGTGATCTCAAATGCGTGAGGCACTTGTGTTGTTACAGTGGAACAAAGCACCAGAAGATCTTCTCTATTTGTATTGGAGGGTAAAGAACGAGGGGGTTGAGGTTCTTTTCCAGATGTGCTAAATAGTGTCTCCCAAAAGCTTCGGGTTTATCTAAGATTGAAAGAGTCGATCATCCTCCAAAATTAGAGCTGTCCCTTGCATAGGTGCTCAAAGATGGCGTTAACATGCTAATTTGCTGTTATAGTGGAAGTAAGCAACCAAAGGTGATCTTCTCTATTTGTACTAGAAGTTAAAGAACAAGGAGGTTGAGGTTCTTTTCGAGATATGTTAAATAGTTTCTCCCAAAAGTGTCTTAAAAGAGCTGATCCTCCAAAATTAGATCTGTCCCCTACAAAGGTGCTTAAAGATGACGTTAGCATGTTAGAATTGGGGGCTGGAAGCAACTGGTGTTTTGGTAGCGAAGGAGAAGGCAACTGATTCTTGGGAGGGAATTGACAATGGAGTGAGGAAGTTATTTGGGGAAGACTTAAGAGTTCGAGAGTTTTGTGGAAAAATTGGTGGTAGTCCTAGGACATGTGAAGAAGCTGTTGTTCTAAGGAGGAACTTGTTAGATTTTGGGCGTGGGATGGTTCTAGACTGGTCTATCAGTTTCCAGCTAAGGGACAAAGTATTAGTGGCCAAAACTCTCTGA

mRNA sequence

ATGGGAAGGGGGAAAGATCTGAATTTAACATGCTCTTCTGAAAGTACAACTGATATTATAGATAGAACTCATCAATCTGTTGGCCCAGAGTTAGGGTCTAAAAATCACATATCCTCCAAAGCCTCCTTATGGTCAGGCTTCTTTACATCCACTTTCTCAATCTTTGAACATCACAAAGAGTCCTCTGTCAGTGAAAAGAAGGCATTTCATTCTCGACACAATGTCTGGGCAACTGTAAGAAAAGTTATGACTAGTGGCTCAATGAGGAGAATTCAGGAGCGCATAATGGGTTCTCGCAGAAGTGGTGTCTATAGCTCGGGTGGTGATATATGGCTTCTTGGTGTGTGCCATAAAATTTCCCAAGATCAGGCTCCTGATGATGCAGTAACTAGCACTGGTGCAGCAGGATATGAGCTAGATTTTTCATCTAGAATTCTGATGACCTATCGTAAAGGTTTTAATGTTATTCAAGACTCAAAATACACCAGTGATGTTAATTGGGGTTGCATGCTAAGAAGCAGCCAAATGCTTGTTGCTCAGCCATTGGATAAAGAATATGTTGAAATTTTGCACCTTTTTGGTGACTCGGAGACATCGCCATTTTCAATTCATAATCTTCTTCAGGCAGGAAGGGCCTATGACCTTGCTGCTGGGTCATGGGTGGGACCATATGCCATGTGTAGGTCATGGGAGACTCTAGTCCGCTTAAAGAGGGAGACCCCTAATCTCCAAGACCAGCAACTTCCAATGGCCATTTACATTGTTTCTGGAGATGAAGATGGAGAGAGAGGTGGTGCTCCAGTTTTATGCATTGATGATGCCTCAAGACATTGTTTTGAGTTTTCAAAAGGCCAACTTGATTGGAGCCCCATTCTTTTATTAGTTCCTTTGGTCCTTGGACTTGAAAAAATCAACCCAAGGTACATTCCATCATTGCGAACAACATTTACCTTTCCCCAAAGCCTAGGGATCTTGGGAGGGAAGCCTGGTGCTTCAACATACATAGTGGGCGTCCAAGATGAAAATGCCTTTTACCTTGATCCACATGAAGTTCAGCAGAATTGGGGGCTGGAAGCAACTGGTGTTTTGGTAGCGAAGGAGAAGGCAACTGATTCTTGGGAGGGAATTGACAATGGAGTGAGGAAGTTATTTGGGGAAGACTTAAGAGTTCGAGAGTTTTGTGGAAAAATTGGTGGTAGTCCTAGGACATGTGAAGAAGCTGTTGTTCTAAGGAGGAACTTGTTAGATTTTGGGCGTGGGATGGTTCTAGACTGGTCTATCAGTTTCCAGCTAAGGGACAAAGTATTAGTGGCCAAAACTCTCTGA

Coding sequence (CDS)

ATGGGAAGGGGGAAAGATCTGAATTTAACATGCTCTTCTGAAAGTACAACTGATATTATAGATAGAACTCATCAATCTGTTGGCCCAGAGTTAGGGTCTAAAAATCACATATCCTCCAAAGCCTCCTTATGGTCAGGCTTCTTTACATCCACTTTCTCAATCTTTGAACATCACAAAGAGTCCTCTGTCAGTGAAAAGAAGGCATTTCATTCTCGACACAATGTCTGGGCAACTGTAAGAAAAGTTATGACTAGTGGCTCAATGAGGAGAATTCAGGAGCGCATAATGGGTTCTCGCAGAAGTGGTGTCTATAGCTCGGGTGGTGATATATGGCTTCTTGGTGTGTGCCATAAAATTTCCCAAGATCAGGCTCCTGATGATGCAGTAACTAGCACTGGTGCAGCAGGATATGAGCTAGATTTTTCATCTAGAATTCTGATGACCTATCGTAAAGGTTTTAATGTTATTCAAGACTCAAAATACACCAGTGATGTTAATTGGGGTTGCATGCTAAGAAGCAGCCAAATGCTTGTTGCTCAGCCATTGGATAAAGAATATGTTGAAATTTTGCACCTTTTTGGTGACTCGGAGACATCGCCATTTTCAATTCATAATCTTCTTCAGGCAGGAAGGGCCTATGACCTTGCTGCTGGGTCATGGGTGGGACCATATGCCATGTGTAGGTCATGGGAGACTCTAGTCCGCTTAAAGAGGGAGACCCCTAATCTCCAAGACCAGCAACTTCCAATGGCCATTTACATTGTTTCTGGAGATGAAGATGGAGAGAGAGGTGGTGCTCCAGTTTTATGCATTGATGATGCCTCAAGACATTGTTTTGAGTTTTCAAAAGGCCAACTTGATTGGAGCCCCATTCTTTTATTAGTTCCTTTGGTCCTTGGACTTGAAAAAATCAACCCAAGGTACATTCCATCATTGCGAACAACATTTACCTTTCCCCAAAGCCTAGGGATCTTGGGAGGGAAGCCTGGTGCTTCAACATACATAGTGGGCGTCCAAGATGAAAATGCCTTTTACCTTGATCCACATGAAGTTCAGCAGAATTGGGGGCTGGAAGCAACTGGTGTTTTGGTAGCGAAGGAGAAGGCAACTGATTCTTGGGAGGGAATTGACAATGGAGTGAGGAAGTTATTTGGGGAAGACTTAAGAGTTCGAGAGTTTTGTGGAAAAATTGGTGGTAGTCCTAGGACATGTGAAGAAGCTGTTGTTCTAAGGAGGAACTTGTTAGATTTTGGGCGTGGGATGGTTCTAGACTGGTCTATCAGTTTCCAGCTAAGGGACAAAGTATTAGTGGCCAAAACTCTCTGA

Protein sequence

MGRGKDLNLTCSSESTTDIIDRTHQSVGPELGSKNHISSKASLWSGFFTSTFSIFEHHKESSVSEKKAFHSRHNVWATVRKVMTSGSMRRIQERIMGSRRSGVYSSGGDIWLLGVCHKISQDQAPDDAVTSTGAAGYELDFSSRILMTYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQPLDKEYVEILHLFGDSETSPFSIHNLLQAGRAYDLAAGSWVGPYAMCRSWETLVRLKRETPNLQDQQLPMAIYIVSGDEDGERGGAPVLCIDDASRHCFEFSKGQLDWSPILLLVPLVLGLEKINPRYIPSLRTTFTFPQSLGILGGKPGASTYIVGVQDENAFYLDPHEVQQNWGLEATGVLVAKEKATDSWEGIDNGVRKLFGEDLRVREFCGKIGGSPRTCEEAVVLRRNLLDFGRGMVLDWSISFQLRDKVLVAKTL

Homology

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BLAST of Lsi09G010710 vs. ExPASy Swiss-Prot
Match: A2Q1V6 (Cysteine protease ATG4 OS=Medicago truncatula OX=3880 GN=ATG4 PE=3 SV=1)

HSP 1 Score: 466.1 bits (1198), Expect = 4.5e-130
Identity = 230/360 (63.89%), Postives = 275/360 (76.39%), Query Frame = 0


Query: 11  CSSESTTDIIDRTHQSVGPELGSKNHISSKASLWSGFFTSTFSIFEHHKESSVSEKKAFH 70
           CSS+S+T+I+D T      + GS +    KASLWS FFTS FS+ E + ESS SEKK  H
Sbjct: 15  CSSKSSTEIVDNTQVPASSKAGSSDSKFPKASLWSTFFTSGFSVDETYSESSSSEKKTVH 74

Query: 71  SRHNVW-ATVRKVMTSGSMRRIQERIMGSRRSGVYSSGGDIWLLGVCHKISQDQAPDDAV 130
           SR++ W A VRKV++ GSMRR QER++GS R+ V SS GDIWLLGVCHKISQ ++  D  
Sbjct: 75  SRNSGWAAAVRKVVSGGSMRRFQERVLGSCRTDVSSSDGDIWLLGVCHKISQHESTGDVD 134

Query: 131 TSTGAAGYELDFSSRILMTYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQ--------- 190
                A +E DF SRIL+TYRKGF+ I+DSKYTSDVNWGCMLRSSQMLVAQ         
Sbjct: 135 IRNVFAAFEQDFFSRILITYRKGFDAIEDSKYTSDVNWGCMLRSSQMLVAQALLFHKLGR 194

Query: 191 --------PLDKEYVEILHLFGDSETSPFSIHNLLQAGRAYDLAAGSWVGPYAMCRSWET 250
                   P+DKEY++IL LFGDSE + FSIHNLLQAG+ Y LA GSWVGPYAMCR+WE 
Sbjct: 195 SWRKTVDKPVDKEYIDILQLFGDSEAAAFSIHNLLQAGKGYGLAVGSWVGPYAMCRTWEV 254

Query: 251 LVRLKRETPNLQDQQLPMAIYIVSGDEDGERGGAPVLCIDDASRHCFEFSKGQLDWSPIL 310
           L R +RE     +Q LPMAIY+VSGDEDGERGGAPV+CI+DA + C EFS+G + W+P+L
Sbjct: 255 LARNQREKNEQGEQLLPMAIYVVSGDEDGERGGAPVVCIEDACKRCLEFSRGLVPWTPLL 314

Query: 311 LLVPLVLGLEKINPRYIPSLRTTFTFPQSLGILGGKPGASTYIVGVQDENAFYLDPHEVQ 353
           LLVPLVLGL+K+N RYIP L++TF FPQSLGILGGKPGASTYI+GVQ++ AFYLDPHEV+
Sbjct: 315 LLVPLVLGLDKVNLRYIPLLQSTFKFPQSLGILGGKPGASTYIIGVQNDKAFYLDPHEVK 374

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BLAST of Lsi09G010710 vs. ExPASy Swiss-Prot
Match: Q8S929 (Cysteine protease ATG4a OS=Arabidopsis thaliana OX=3702 GN=ATG4A PE=2 SV=1)

HSP 1 Score: 410.2 bits (1053), Expect = 2.9e-113
Identity = 207/361 (57.34%), Postives = 265/361 (73.41%), Query Frame = 0


Query: 11  CSSESTTDIIDRTHQSVGPELGSKNHISSKASLWSGFFTSTFSIFEHHKESSVSEKKAFH 70
           CSS S +D  D++   +  + G  ++  SK +LWS  FTS+ S+ + ++ESS S  K   
Sbjct: 13  CSSSSKSDTHDKS--PLVSDSGPSDN-KSKFTLWSNVFTSSSSVSQPYRESSTSGHKQVC 72

Query: 71  SRHNVW-ATVRKV-MTSGSMRRIQERIMGSRRSGVYSSGGDIWLLGVCHKISQDQAPDDA 130
           +  N W A V++V M SG++RR QER++G  R+G+ S+  D+WLLGVC+KIS D+   + 
Sbjct: 73  TTRNGWTAFVKRVSMASGAIRRFQERVLGPNRTGLPSTTSDVWLLGVCYKISADENSGET 132

Query: 131 VTSTGAAGYELDFSSRILMTYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQPL------ 190
            T T  A  +LDFSS+ILMTYRKGF   +D+ YTSDVNWGCM+RSSQML AQ L      
Sbjct: 133 DTGTVLAALQLDFSSKILMTYRKGFEPFRDTTYTSDVNWGCMIRSSQMLFAQALLFHRLG 192

Query: 191 ----------DKEYVEILHLFGDSETSPFSIHNLLQAGRAYDLAAGSWVGPYAMCRSWET 250
                     ++EY+E L  FGDSE S FSIHNL+ AG +Y LAAGSWVGPYA+CR+WE+
Sbjct: 193 RAWTKKSELPEQEYLETLEPFGDSEPSAFSIHNLIIAGASYGLAAGSWVGPYAICRAWES 252

Query: 251 LVRLKRETPNLQDQQLPMAIYIVSGDEDGERGGAPVLCIDDASRHCFEFSKGQLDWSPIL 310
           L   KR+  + ++Q LPMA++IVSG EDGERGGAP+LCI+DA++ C EFSKGQ +W+PI+
Sbjct: 253 LACKKRKQTDSKNQTLPMAVHIVSGSEDGERGGAPILCIEDATKSCLEFSKGQSEWTPII 312

Query: 311 LLVPLVLGLEKINPRYIPSLRTTFTFPQSLGILGGKPGASTYIVGVQDENAFYLDPHEVQ 354
           LLVPLVLGL+ +NPRYIPSL  TFTFPQS+GILGGKPGASTYIVGVQ++  FYLDPHEVQ
Sbjct: 313 LLVPLVLGLDSVNPRYIPSLVATFTFPQSVGILGGKPGASTYIVGVQEDKGFYLDPHEVQ 370

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BLAST of Lsi09G010710 vs. ExPASy Swiss-Prot
Match: Q9M1Y0 (Cysteine protease ATG4b OS=Arabidopsis thaliana OX=3702 GN=ATG4B PE=1 SV=1)

HSP 1 Score: 385.2 bits (988), Expect = 1.0e-105
Identity = 191/362 (52.76%), Postives = 250/362 (69.06%), Query Frame = 0


Query: 11  CSSESTTDIIDRTHQSVGPELGSKNHISSKASLWSGFFTSTFSIFEHHKESSVSEKKAFH 70
           CSS ST++  D +  +      + +   S  +L S    S+  + +  +E+S S      
Sbjct: 13  CSSSSTSEKRDISSPTSLVSDSASSDNKSNLTLCSDVVASSSPVSQLCREASTSGHNPVC 72

Query: 71  SRHNVWATVRKV--MTSGSMRRIQERIMGSRRSGVYSSGGDIWLLGVCHKISQDQAPDDA 130
           + H+ W  + K   M SG++RR Q+R++G  R+G+ SS  +IWLLGVC+KIS+ ++ ++A
Sbjct: 73  TTHSSWTVILKTASMASGAIRRFQDRVLGPSRTGISSSTSEIWLLGVCYKISEGESSEEA 132

Query: 131 VTSTGAAGYELDFSSRILMTYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQ-------- 190
                 A +  DFSS ILMTYR+GF  I D+ YTSDVNWGCMLRS QML AQ        
Sbjct: 133 DAGRVLAAFRQDFSSLILMTYRRGFEPIGDTTYTSDVNWGCMLRSGQMLFAQALLFQRLG 192

Query: 191 ---------PLDKEYVEILHLFGDSETSPFSIHNLLQAGRAYDLAAGSWVGPYAMCRSWE 250
                    P D++Y+EIL LFGD+E S FSIHNL+ AG +Y LAAGSWVGPYA+CRSWE
Sbjct: 193 RSWRKKDSEPADEKYLEILELFGDTEASAFSIHNLILAGESYGLAAGSWVGPYAVCRSWE 252

Query: 251 TLVRLKRETPNLQDQQLPMAIYIVSGDEDGERGGAPVLCIDDASRHCFEFSKGQLDWSPI 310
           +L R  +E  + + +   MA++IVSG EDGERGGAP+LCI+D ++ C EFS+G+ +W PI
Sbjct: 253 SLARKNKEETDDKHKSFSMAVHIVSGSEDGERGGAPILCIEDVTKTCLEFSEGETEWPPI 312

Query: 311 LLLVPLVLGLEKINPRYIPSLRTTFTFPQSLGILGGKPGASTYIVGVQDENAFYLDPHEV 354
           LLLVPLVLGL+++NPRYIPSL  TFTFPQSLGILGGKPGASTYIVGVQ++  FYLDPH+V
Sbjct: 313 LLLVPLVLGLDRVNPRYIPSLIATFTFPQSLGILGGKPGASTYIVGVQEDKGFYLDPHDV 372

[Close]

BLAST of Lsi09G010710 vs. ExPASy Swiss-Prot
Match: Q7XPW8 (Cysteine protease ATG4B OS=Oryza sativa subsp. japonica OX=39947 GN=ATG4B PE=2 SV=1)

HSP 1 Score: 384.0 bits (985), Expect = 2.2e-105
Identity = 197/344 (57.27%), Postives = 245/344 (71.22%), Query Frame = 0


Query: 39  SKASLWSGFFTSTFSIFEHHKESSVSEKKAFHSRHNVWATV-RKVMTSGSMRRIQERIMG 98
           SK S+ S  F S F+IFE H++SS ++     S    W+ V R+++ SGSM     R +G
Sbjct: 40  SKTSILSCVFNSPFNIFEAHQDSSANKSPKSSSGSYDWSRVLRRIVCSGSM----WRFLG 99

Query: 99  SRRSGVYSSGGDIWLLGVCHKISQDQAPDDAVTSTGAAGYELDFSSRILMTYRKGFNVIQ 158
           + +     +  D+W LG C+K+S +++  D+ + +G A +  DFSSRI +TYR+GF+ I 
Sbjct: 100 TSK---VLTSSDVWFLGKCYKLSSEESSSDSDSESGHATFLEDFSSRIWITYRRGFDAIS 159

Query: 159 DSKYTSDVNWGCMLRSSQMLVAQ-------------PLDK----EYVEILHLFGDSETSP 218
           DSKYTSDVNWGCM+RSSQMLVAQ             PL+K    EY+ ILH+FGDSE   
Sbjct: 160 DSKYTSDVNWGCMVRSSQMLVAQALIFHHLGRSWRRPLEKPYNPEYIGILHMFGDSEACA 219

Query: 219 FSIHNLLQAGRAYDLAAGSWVGPYAMCRSWETLVRLKRETPNLQD--QQLPMAIYIVSGD 278
           FSIHNLLQAG +Y LAAGSWVGPYAMCR+W+TLVR  RE   + D  +  PMA+Y+VSGD
Sbjct: 220 FSIHNLLQAGNSYGLAAGSWVGPYAMCRAWQTLVRTNREQHEVVDGNESFPMALYVVSGD 279

Query: 279 EDGERGGAPVLCIDDASRHCFEFSKGQLDWSPILLLVPLVLGLEKINPRYIPSLRTTFTF 338
           EDGERGGAPV+CID A++ C +F+KGQ  WSPILLLVPLVLGL+KINPRYIP L+ TFTF
Sbjct: 280 EDGERGGAPVVCIDVAAQLCCDFNKGQSTWSPILLLVPLVLGLDKINPRYIPLLKETFTF 339

Query: 339 PQSLGILGGKPGASTYIVGVQDENAFYLDPHEVQQNWGLEATGV 363
           PQSLGILGGKPG STYI GVQD+ A YLDPHEVQ    + A  +
Sbjct: 340 PQSLGILGGKPGTSTYIAGVQDDRALYLDPHEVQMAVDIAADNI 376

[Close]

BLAST of Lsi09G010710 vs. ExPASy Swiss-Prot
Match: Q2XPP4 (Cysteine protease ATG4B OS=Oryza sativa subsp. indica OX=39946 GN=ATG4B PE=1 SV=2)

HSP 1 Score: 382.9 bits (982), Expect = 5.0e-105
Identity = 195/344 (56.69%), Postives = 242/344 (70.35%), Query Frame = 0


Query: 39  SKASLWSGFFTSTFSIFEHHKESSVSEKKAFHSRHNVWATV-RKVMTSGSMRRIQERIMG 98
           SK S+ S  F S F+IFE H++SS ++     S    W  V R+++ SGSM     R +G
Sbjct: 40  SKTSILSCVFNSPFNIFEAHQDSSANKSPKSSSGSYDWLRVLRRIVCSGSM----WRFLG 99

Query: 99  SRRSGVYSSGGDIWLLGVCHKISQDQAPDDAVTSTGAAGYELDFSSRILMTYRKGFNVIQ 158
           + +     +  D+W LG C+K+S +++  D+ + +G A +  DFSSRI +TYR+GF+ I 
Sbjct: 100 TSK---VLTSSDVWFLGKCYKLSSEESSSDSDSESGHATFLEDFSSRIWITYRRGFDAIS 159

Query: 159 DSKYTSDVNWGCMLRSSQMLVAQ-----------------PLDKEYVEILHLFGDSETSP 218
           DSKYTSDVNWGCM+RSSQMLVAQ                 P + EY+ ILH+FGDSE   
Sbjct: 160 DSKYTSDVNWGCMVRSSQMLVAQALIFHHLGRSWRRPSEKPYNPEYIGILHMFGDSEACA 219

Query: 219 FSIHNLLQAGRAYDLAAGSWVGPYAMCRSWETLVRLKRETPNLQD--QQLPMAIYIVSGD 278
           FSIHNLLQAG +Y LAAGSWVGPYAMCR+W+TLVR  RE   + D  +  PMA+Y+VSGD
Sbjct: 220 FSIHNLLQAGNSYGLAAGSWVGPYAMCRAWQTLVRTNREQHEVVDGNESFPMALYVVSGD 279

Query: 279 EDGERGGAPVLCIDDASRHCFEFSKGQLDWSPILLLVPLVLGLEKINPRYIPSLRTTFTF 338
           EDGERGGAPV+CID A++ C +F+KGQ  WSPILLLVPLVLGL+KINPRYIP L+ TFTF
Sbjct: 280 EDGERGGAPVVCIDVAAQLCCDFNKGQSTWSPILLLVPLVLGLDKINPRYIPLLKETFTF 339

Query: 339 PQSLGILGGKPGASTYIVGVQDENAFYLDPHEVQQNWGLEATGV 363
           PQSLGILGGKPG STYI GVQD+ A YLDPHEVQ    + A  +
Sbjct: 340 PQSLGILGGKPGTSTYIAGVQDDRALYLDPHEVQMAVDIAADNI 376

[Close]

BLAST of Lsi09G010710 vs. ExPASy TrEMBL
Match: A0A5A7SP82 (Cysteine protease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold340G00200 PE=3 SV=1)

HSP 1 Score: 654.8 bits (1688), Expect = 2.5e-184
Identity = 323/370 (87.30%), Postives = 334/370 (90.27%), Query Frame = 0


Query: 1   MGRGKDLNLTCSSESTTDIIDRTHQSVGPELGSKNHISSKASLWSGFFTSTFSIFEHHKE 60
           MGRGKDL  TCSSE+T D+IDRTH+SV  ELGSKNHISSKASLWSGFF+S FSI +HHK+
Sbjct: 1   MGRGKDLKSTCSSETTADVIDRTHRSVCSELGSKNHISSKASLWSGFFSSNFSICDHHKD 60

Query: 61  SSVSEKKAFHSRHNVWATVRKVMTSGSMRRIQERIMGSRRSGVYSSGGDIWLLGVCHKIS 120
           SSVSEKK FHSRHNVWATVRKVMTSGSMRRIQERI+GSRRSGVY+SGGDIWLLGVCHKIS
Sbjct: 61  SSVSEKKVFHSRHNVWATVRKVMTSGSMRRIQERILGSRRSGVYTSGGDIWLLGVCHKIS 120

Query: 121 QDQAPDDAVTSTGAAGYELDFSSRILMTYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQ 180
           QD  PDDA +STG AG+E DFSSRILMTYRKGFNVIQDSKYTSDVNWGCMLRSSQMLV+Q
Sbjct: 121 QDHLPDDAASSTGVAGFEQDFSSRILMTYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVSQ 180

Query: 181 -----------------PLDKEYVEILHLFGDSETSPFSIHNLLQAGRAYDLAAGSWVGP 240
                            P DKEYVEILHLFGDSETS FSIHNLLQAGRAYDLAAGSWVGP
Sbjct: 181 ALLFHRLGRSWRKPSQKPFDKEYVEILHLFGDSETSAFSIHNLLQAGRAYDLAAGSWVGP 240

Query: 241 YAMCRSWETLVRLKRETPNLQDQQLPMAIYIVSGDEDGERGGAPVLCIDDASRHCFEFSK 300
           YAMCRSWETLVR KRETP LQDQQLPMAIYIVSGDEDGERGGAPVL IDDASRHCFEFSK
Sbjct: 241 YAMCRSWETLVRSKRETPILQDQQLPMAIYIVSGDEDGERGGAPVLYIDDASRHCFEFSK 300

Query: 301 GQLDWSPILLLVPLVLGLEKINPRYIPSLRTTFTFPQSLGILGGKPGASTYIVGVQDENA 354
           GQ DWSPILLLVPLVLGLEKINPRYIPSLRTTFTFPQSLGILGGKPGASTYIVGVQDENA
Sbjct: 301 GQHDWSPILLLVPLVLGLEKINPRYIPSLRTTFTFPQSLGILGGKPGASTYIVGVQDENA 360

[Close]

BLAST of Lsi09G010710 vs. ExPASy TrEMBL
Match: A0A1S3B4R1 (Cysteine protease OS=Cucumis melo OX=3656 GN=LOC103485762 PE=3 SV=1)

HSP 1 Score: 654.8 bits (1688), Expect = 2.5e-184
Identity = 323/370 (87.30%), Postives = 334/370 (90.27%), Query Frame = 0


Query: 1   MGRGKDLNLTCSSESTTDIIDRTHQSVGPELGSKNHISSKASLWSGFFTSTFSIFEHHKE 60
           MGRGKDL  TCSSE+T D+IDRTH+SV  ELGSKNHISSKASLWSGFF+S FSI +HHK+
Sbjct: 1   MGRGKDLKSTCSSETTADVIDRTHRSVCSELGSKNHISSKASLWSGFFSSNFSICDHHKD 60

Query: 61  SSVSEKKAFHSRHNVWATVRKVMTSGSMRRIQERIMGSRRSGVYSSGGDIWLLGVCHKIS 120
           SSVSEKK FHSRHNVWATVRKVMTSGSMRRIQERI+GSRRSGVY+SGGDIWLLGVCHKIS
Sbjct: 61  SSVSEKKVFHSRHNVWATVRKVMTSGSMRRIQERILGSRRSGVYTSGGDIWLLGVCHKIS 120

Query: 121 QDQAPDDAVTSTGAAGYELDFSSRILMTYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQ 180
           QD  PDDA +STG AG+E DFSSRILMTYRKGFNVIQDSKYTSDVNWGCMLRSSQMLV+Q
Sbjct: 121 QDHLPDDAASSTGVAGFEQDFSSRILMTYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVSQ 180

Query: 181 -----------------PLDKEYVEILHLFGDSETSPFSIHNLLQAGRAYDLAAGSWVGP 240
                            P DKEYVEILHLFGDSETS FSIHNLLQAGRAYDLAAGSWVGP
Sbjct: 181 ALLFHRLGRSWRKPSQKPFDKEYVEILHLFGDSETSAFSIHNLLQAGRAYDLAAGSWVGP 240

Query: 241 YAMCRSWETLVRLKRETPNLQDQQLPMAIYIVSGDEDGERGGAPVLCIDDASRHCFEFSK 300
           YAMCRSWETLVR KRETP LQDQQLPMAIYIVSGDEDGERGGAPVL IDDASRHCFEFSK
Sbjct: 241 YAMCRSWETLVRSKRETPILQDQQLPMAIYIVSGDEDGERGGAPVLYIDDASRHCFEFSK 300

Query: 301 GQLDWSPILLLVPLVLGLEKINPRYIPSLRTTFTFPQSLGILGGKPGASTYIVGVQDENA 354
           GQ DWSPILLLVPLVLGLEKINPRYIPSLRTTFTFPQSLGILGGKPGASTYIVGVQDENA
Sbjct: 301 GQHDWSPILLLVPLVLGLEKINPRYIPSLRTTFTFPQSLGILGGKPGASTYIVGVQDENA 360

[Close]

BLAST of Lsi09G010710 vs. ExPASy TrEMBL
Match: A0A6J1ETR9 (Cysteine protease OS=Cucurbita moschata OX=3662 GN=LOC111436439 PE=3 SV=1)

HSP 1 Score: 652.5 bits (1682), Expect = 1.2e-183
Identity = 322/370 (87.03%), Postives = 334/370 (90.27%), Query Frame = 0


Query: 1   MGRGKDLNLTCSSESTTDIIDRTHQSVGPELGSKNHISSKASLWSGFFTSTFSIFEHHKE 60
           MGRGKDLN TCSSES TDI+DR+ QS+ P LGS+NHISSKASLWSGFFTSTFS+FEH+KE
Sbjct: 1   MGRGKDLNSTCSSESVTDIVDRSQQSICPTLGSRNHISSKASLWSGFFTSTFSVFEHNKE 60

Query: 61  SSVSEKKAFHSRHNVWATVRKVMTSGSMRRIQERIMGSRRSGVYSSGGDIWLLGVCHKIS 120
           SSVSEKKA HSRHNVW TVR+VMTSGSMRRIQERI+GSRRSGVYSSGGDIWLLGVCHKIS
Sbjct: 61  SSVSEKKAVHSRHNVWTTVRRVMTSGSMRRIQERILGSRRSGVYSSGGDIWLLGVCHKIS 120

Query: 121 QDQAPDDAVTSTGAAGYELDFSSRILMTYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQ 180
           QDQA DDAVTS   AG+ELDFSSRILMTYRKGFN IQDSKYTSDVNWGCMLRSSQMLVAQ
Sbjct: 121 QDQASDDAVTSDSVAGFELDFSSRILMTYRKGFNGIQDSKYTSDVNWGCMLRSSQMLVAQ 180

Query: 181 -----------------PLDKEYVEILHLFGDSETSPFSIHNLLQAGRAYDLAAGSWVGP 240
                            PLDKEYVEILHLFGDSETS FSIHNLLQAGR YDLAAGSWVGP
Sbjct: 181 ALLFHRLGRSWRKTSQKPLDKEYVEILHLFGDSETSAFSIHNLLQAGRPYDLAAGSWVGP 240

Query: 241 YAMCRSWETLVRLKRETPNLQDQQLPMAIYIVSGDEDGERGGAPVLCIDDASRHCFEFSK 300
           YA+CRSWETLVRLKRETP  QDQQLPMAIYIVSGDEDGERGGAPVLCID ASRHCF+FSK
Sbjct: 241 YAICRSWETLVRLKRETPTPQDQQLPMAIYIVSGDEDGERGGAPVLCIDVASRHCFQFSK 300

Query: 301 GQLDWSPILLLVPLVLGLEKINPRYIPSLRTTFTFPQSLGILGGKPGASTYIVGVQDENA 354
           GQLDW+PILLLVPLVLGLEKINPRYIPSLR TFTFPQSLGILGGK GASTYIVGVQDENA
Sbjct: 301 GQLDWTPILLLVPLVLGLEKINPRYIPSLRATFTFPQSLGILGGKAGASTYIVGVQDENA 360

[Close]

BLAST of Lsi09G010710 vs. ExPASy TrEMBL
Match: A0A0A0LK81 (Cysteine protease OS=Cucumis sativus OX=3659 GN=Csa_2G128640 PE=3 SV=1)

HSP 1 Score: 651.0 bits (1678), Expect = 3.6e-183
Identity = 322/370 (87.03%), Postives = 330/370 (89.19%), Query Frame = 0


Query: 1   MGRGKDLNLTCSSESTTDIIDRTHQSVGPELGSKNHISSKASLWSGFFTSTFSIFEHHKE 60
           MGRGKDL  TCS E   D IDRTH+SV PELGSKNHISSKAS WSGFF+S FSIFEHHK+
Sbjct: 1   MGRGKDLKSTCSPEPAADAIDRTHRSVYPELGSKNHISSKASSWSGFFSSNFSIFEHHKD 60

Query: 61  SSVSEKKAFHSRHNVWATVRKVMTSGSMRRIQERIMGSRRSGVYSSGGDIWLLGVCHKIS 120
           SSV+EKK FH RHNVWATVRKVMTSGSMRRIQER++GSRRSGVYSSGGDIWLLGVCHKIS
Sbjct: 61  SSVTEKKVFHPRHNVWATVRKVMTSGSMRRIQERLLGSRRSGVYSSGGDIWLLGVCHKIS 120

Query: 121 QDQAPDDAVTSTGAAGYELDFSSRILMTYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQ 180
           QD  PDDA +S G AGYE DFSSRILMTYRKGF+VIQDSKYTSDVNWGCMLRSSQMLVAQ
Sbjct: 121 QDHPPDDAASSPGVAGYEQDFSSRILMTYRKGFHVIQDSKYTSDVNWGCMLRSSQMLVAQ 180

Query: 181 -----------------PLDKEYVEILHLFGDSETSPFSIHNLLQAGRAYDLAAGSWVGP 240
                            PLDKEYVEILHLFGDSETS FSIHNLLQAGRAYDLAAGSWVGP
Sbjct: 181 ALLFHRLGRSWRKPSQKPLDKEYVEILHLFGDSETSAFSIHNLLQAGRAYDLAAGSWVGP 240

Query: 241 YAMCRSWETLVRLKRETPNLQDQQLPMAIYIVSGDEDGERGGAPVLCIDDASRHCFEFSK 300
           YAMCRSWETLVR KRETP LQDQQLPMAIYIVSGDEDGERGGAPVL IDDASRHCFEFSK
Sbjct: 241 YAMCRSWETLVRSKRETPILQDQQLPMAIYIVSGDEDGERGGAPVLYIDDASRHCFEFSK 300

Query: 301 GQLDWSPILLLVPLVLGLEKINPRYIPSLRTTFTFPQSLGILGGKPGASTYIVGVQDENA 354
           GQ DWSPILLLVPLVLGLEKINPRYIPSLRTTFTFPQSLGILGGKPGASTYIVGVQDENA
Sbjct: 301 GQHDWSPILLLVPLVLGLEKINPRYIPSLRTTFTFPQSLGILGGKPGASTYIVGVQDENA 360

[Close]

BLAST of Lsi09G010710 vs. ExPASy TrEMBL
Match: A0A6J1ICU1 (Cysteine protease OS=Cucurbita maxima OX=3661 GN=LOC111472623 PE=3 SV=1)

HSP 1 Score: 650.6 bits (1677), Expect = 4.7e-183
Identity = 320/370 (86.49%), Postives = 333/370 (90.00%), Query Frame = 0


Query: 1   MGRGKDLNLTCSSESTTDIIDRTHQSVGPELGSKNHISSKASLWSGFFTSTFSIFEHHKE 60
           MGRGKDLN TCSSES TDI+DR+ QS+ P LGS+NHISSKASLWSGFFTSTFS+FEH+KE
Sbjct: 1   MGRGKDLNSTCSSESVTDIVDRSQQSICPTLGSRNHISSKASLWSGFFTSTFSVFEHNKE 60

Query: 61  SSVSEKKAFHSRHNVWATVRKVMTSGSMRRIQERIMGSRRSGVYSSGGDIWLLGVCHKIS 120
           SSVSEKKA HSRHNVW TVR+VMTSGSMRRIQERI+GSRRSGVYSSGGDIWLLGVCHKIS
Sbjct: 61  SSVSEKKAVHSRHNVWTTVRRVMTSGSMRRIQERILGSRRSGVYSSGGDIWLLGVCHKIS 120

Query: 121 QDQAPDDAVTSTGAAGYELDFSSRILMTYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQ 180
           QDQA DD VTS   AG+ELDFSSRILMTYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQ
Sbjct: 121 QDQASDDVVTSDSIAGFELDFSSRILMTYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQ 180

Query: 181 -----------------PLDKEYVEILHLFGDSETSPFSIHNLLQAGRAYDLAAGSWVGP 240
                            PLDKEYVEILHLFGDSETS FSIHNLLQAGR YDLAAGSWVGP
Sbjct: 181 ALLFHRLGRSWRKTSQKPLDKEYVEILHLFGDSETSAFSIHNLLQAGRPYDLAAGSWVGP 240

Query: 241 YAMCRSWETLVRLKRETPNLQDQQLPMAIYIVSGDEDGERGGAPVLCIDDASRHCFEFSK 300
           YA+CRSWETLVRLKRETP  QD QLPMAIYIVSG+EDGERGGAPVLCIDDASRHCF+FS 
Sbjct: 241 YAVCRSWETLVRLKRETPTPQDLQLPMAIYIVSGEEDGERGGAPVLCIDDASRHCFQFSN 300

Query: 301 GQLDWSPILLLVPLVLGLEKINPRYIPSLRTTFTFPQSLGILGGKPGASTYIVGVQDENA 354
           GQLDW+PILLLVPLVLGLEKINPRYIPSLR TFTFPQSLGILGGK GASTYIVGVQDENA
Sbjct: 301 GQLDWTPILLLVPLVLGLEKINPRYIPSLRATFTFPQSLGILGGKAGASTYIVGVQDENA 360

[Close]

BLAST of Lsi09G010710 vs. NCBI nr
Match: XP_038874853.1 (cysteine protease ATG4-like [Benincasa hispida])

HSP 1 Score: 696.4 bits (1796), Expect = 1.6e-196
Identity = 343/370 (92.70%), Postives = 348/370 (94.05%), Query Frame = 0


Query: 1   MGRGKDLNLTCSSESTTDIIDRTHQSVGPELGSKNHISSKASLWSGFFTSTFSIFEHHKE 60
           MGRGKDLN TCSSESTT++IDRTHQSV PELGSKNHISSKASLWSGFFTSTFSIFEHHKE
Sbjct: 1   MGRGKDLNSTCSSESTTNVIDRTHQSVCPELGSKNHISSKASLWSGFFTSTFSIFEHHKE 60

Query: 61  SSVSEKKAFHSRHNVWATVRKVMTSGSMRRIQERIMGSRRSGVYSSGGDIWLLGVCHKIS 120
           SSVSEKKAFHSRHNVWATVRKVMTSGSMRRIQERI+GSRRSGVYSSGGDIWLLGVCHKIS
Sbjct: 61  SSVSEKKAFHSRHNVWATVRKVMTSGSMRRIQERILGSRRSGVYSSGGDIWLLGVCHKIS 120

Query: 121 QDQAPDDAVTSTGAAGYELDFSSRILMTYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQ 180
           QDQAPDDAVTSTG AGYELDFSSRILMTYRKGFN IQDSKYTSDVNWGCMLRSSQMLVAQ
Sbjct: 121 QDQAPDDAVTSTGTAGYELDFSSRILMTYRKGFNAIQDSKYTSDVNWGCMLRSSQMLVAQ 180

Query: 181 -----------------PLDKEYVEILHLFGDSETSPFSIHNLLQAGRAYDLAAGSWVGP 240
                            PLDKEYVEILHLFGDSETS FSIHNLLQAGRAYDLAAGSWVGP
Sbjct: 181 ALLFHRLGRSWRKTSQKPLDKEYVEILHLFGDSETSAFSIHNLLQAGRAYDLAAGSWVGP 240

Query: 241 YAMCRSWETLVRLKRETPNLQDQQLPMAIYIVSGDEDGERGGAPVLCIDDASRHCFEFSK 300
           YAMCRSWETLVRLKRETPNLQ+QQLPMAIYIVSGDEDGERGGAPVLCIDDASRHCFEFSK
Sbjct: 241 YAMCRSWETLVRLKRETPNLQEQQLPMAIYIVSGDEDGERGGAPVLCIDDASRHCFEFSK 300

Query: 301 GQLDWSPILLLVPLVLGLEKINPRYIPSLRTTFTFPQSLGILGGKPGASTYIVGVQDENA 354
           GQLDWSPILLLVPLVLGLEKINPRYIPSLRTTFTFPQSLGILGGKPGASTYIVGVQDENA
Sbjct: 301 GQLDWSPILLLVPLVLGLEKINPRYIPSLRTTFTFPQSLGILGGKPGASTYIVGVQDENA 360

[Close]

BLAST of Lsi09G010710 vs. NCBI nr
Match: XP_008441684.1 (PREDICTED: cysteine protease ATG4-like isoform X1 [Cucumis melo] >KAA0032358.1 cysteine protease ATG4-like isoform X1 [Cucumis melo var. makuwa] >TYK31630.1 cysteine protease ATG4-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 654.8 bits (1688), Expect = 5.2e-184
Identity = 323/370 (87.30%), Postives = 334/370 (90.27%), Query Frame = 0


Query: 1   MGRGKDLNLTCSSESTTDIIDRTHQSVGPELGSKNHISSKASLWSGFFTSTFSIFEHHKE 60
           MGRGKDL  TCSSE+T D+IDRTH+SV  ELGSKNHISSKASLWSGFF+S FSI +HHK+
Sbjct: 1   MGRGKDLKSTCSSETTADVIDRTHRSVCSELGSKNHISSKASLWSGFFSSNFSICDHHKD 60

Query: 61  SSVSEKKAFHSRHNVWATVRKVMTSGSMRRIQERIMGSRRSGVYSSGGDIWLLGVCHKIS 120
           SSVSEKK FHSRHNVWATVRKVMTSGSMRRIQERI+GSRRSGVY+SGGDIWLLGVCHKIS
Sbjct: 61  SSVSEKKVFHSRHNVWATVRKVMTSGSMRRIQERILGSRRSGVYTSGGDIWLLGVCHKIS 120

Query: 121 QDQAPDDAVTSTGAAGYELDFSSRILMTYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQ 180
           QD  PDDA +STG AG+E DFSSRILMTYRKGFNVIQDSKYTSDVNWGCMLRSSQMLV+Q
Sbjct: 121 QDHLPDDAASSTGVAGFEQDFSSRILMTYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVSQ 180

Query: 181 -----------------PLDKEYVEILHLFGDSETSPFSIHNLLQAGRAYDLAAGSWVGP 240
                            P DKEYVEILHLFGDSETS FSIHNLLQAGRAYDLAAGSWVGP
Sbjct: 181 ALLFHRLGRSWRKPSQKPFDKEYVEILHLFGDSETSAFSIHNLLQAGRAYDLAAGSWVGP 240

Query: 241 YAMCRSWETLVRLKRETPNLQDQQLPMAIYIVSGDEDGERGGAPVLCIDDASRHCFEFSK 300
           YAMCRSWETLVR KRETP LQDQQLPMAIYIVSGDEDGERGGAPVL IDDASRHCFEFSK
Sbjct: 241 YAMCRSWETLVRSKRETPILQDQQLPMAIYIVSGDEDGERGGAPVLYIDDASRHCFEFSK 300

Query: 301 GQLDWSPILLLVPLVLGLEKINPRYIPSLRTTFTFPQSLGILGGKPGASTYIVGVQDENA 354
           GQ DWSPILLLVPLVLGLEKINPRYIPSLRTTFTFPQSLGILGGKPGASTYIVGVQDENA
Sbjct: 301 GQHDWSPILLLVPLVLGLEKINPRYIPSLRTTFTFPQSLGILGGKPGASTYIVGVQDENA 360

[Close]

BLAST of Lsi09G010710 vs. NCBI nr
Match: XP_023539406.1 (cysteine protease ATG4-like [Cucurbita pepo subsp. pepo] >XP_023539414.1 cysteine protease ATG4-like [Cucurbita pepo subsp. pepo] >XP_023539422.1 cysteine protease ATG4-like [Cucurbita pepo subsp. pepo] >XP_023539429.1 cysteine protease ATG4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 654.4 bits (1687), Expect = 6.8e-184
Identity = 322/370 (87.03%), Postives = 335/370 (90.54%), Query Frame = 0


Query: 1   MGRGKDLNLTCSSESTTDIIDRTHQSVGPELGSKNHISSKASLWSGFFTSTFSIFEHHKE 60
           MGRGKDLN TCSS+S TDI+DR+ QS+ P LGS+NHISSKASLWSGFFTSTFS+FEH+KE
Sbjct: 1   MGRGKDLNSTCSSKSVTDIVDRSQQSICPTLGSRNHISSKASLWSGFFTSTFSVFEHNKE 60

Query: 61  SSVSEKKAFHSRHNVWATVRKVMTSGSMRRIQERIMGSRRSGVYSSGGDIWLLGVCHKIS 120
           SSVSEKKA HSRHNVW TVR+VMTSGSMRRIQERI+GSRRSGVYSSGGDIWLLGVCHKIS
Sbjct: 61  SSVSEKKAVHSRHNVWTTVRRVMTSGSMRRIQERILGSRRSGVYSSGGDIWLLGVCHKIS 120

Query: 121 QDQAPDDAVTSTGAAGYELDFSSRILMTYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQ 180
           QDQA DDAVTS   AG+ELDFSSRILMTYRKGFN IQDSKYTSDVNWGCMLRSSQMLVAQ
Sbjct: 121 QDQASDDAVTSDSVAGFELDFSSRILMTYRKGFNDIQDSKYTSDVNWGCMLRSSQMLVAQ 180

Query: 181 -----------------PLDKEYVEILHLFGDSETSPFSIHNLLQAGRAYDLAAGSWVGP 240
                            PLDKEYVEILHLFGDSETS FSIHNLLQAGR YDLAAGSWVGP
Sbjct: 181 ALLFHRLGRSWRKTSQKPLDKEYVEILHLFGDSETSAFSIHNLLQAGRPYDLAAGSWVGP 240

Query: 241 YAMCRSWETLVRLKRETPNLQDQQLPMAIYIVSGDEDGERGGAPVLCIDDASRHCFEFSK 300
           YA+CRSWETLVRLKRETP  QDQQLPMAIYIVSGDEDGERGGAPVLCIDDASRHCF+FSK
Sbjct: 241 YAICRSWETLVRLKRETPTPQDQQLPMAIYIVSGDEDGERGGAPVLCIDDASRHCFQFSK 300

Query: 301 GQLDWSPILLLVPLVLGLEKINPRYIPSLRTTFTFPQSLGILGGKPGASTYIVGVQDENA 354
           GQLDW+PILLLVPLVLGLEKINPRYIPSLR TFTFPQSLGILGGK GASTYIVGVQDENA
Sbjct: 301 GQLDWTPILLLVPLVLGLEKINPRYIPSLRATFTFPQSLGILGGKAGASTYIVGVQDENA 360

[Close]

BLAST of Lsi09G010710 vs. NCBI nr
Match: XP_022929983.1 (cysteine protease ATG4-like [Cucurbita moschata] >XP_022929984.1 cysteine protease ATG4-like [Cucurbita moschata] >XP_022929986.1 cysteine protease ATG4-like [Cucurbita moschata] >XP_022929987.1 cysteine protease ATG4-like [Cucurbita moschata] >XP_022929988.1 cysteine protease ATG4-like [Cucurbita moschata])

HSP 1 Score: 652.5 bits (1682), Expect = 2.6e-183
Identity = 322/370 (87.03%), Postives = 334/370 (90.27%), Query Frame = 0


Query: 1   MGRGKDLNLTCSSESTTDIIDRTHQSVGPELGSKNHISSKASLWSGFFTSTFSIFEHHKE 60
           MGRGKDLN TCSSES TDI+DR+ QS+ P LGS+NHISSKASLWSGFFTSTFS+FEH+KE
Sbjct: 1   MGRGKDLNSTCSSESVTDIVDRSQQSICPTLGSRNHISSKASLWSGFFTSTFSVFEHNKE 60

Query: 61  SSVSEKKAFHSRHNVWATVRKVMTSGSMRRIQERIMGSRRSGVYSSGGDIWLLGVCHKIS 120
           SSVSEKKA HSRHNVW TVR+VMTSGSMRRIQERI+GSRRSGVYSSGGDIWLLGVCHKIS
Sbjct: 61  SSVSEKKAVHSRHNVWTTVRRVMTSGSMRRIQERILGSRRSGVYSSGGDIWLLGVCHKIS 120

Query: 121 QDQAPDDAVTSTGAAGYELDFSSRILMTYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQ 180
           QDQA DDAVTS   AG+ELDFSSRILMTYRKGFN IQDSKYTSDVNWGCMLRSSQMLVAQ
Sbjct: 121 QDQASDDAVTSDSVAGFELDFSSRILMTYRKGFNGIQDSKYTSDVNWGCMLRSSQMLVAQ 180

Query: 181 -----------------PLDKEYVEILHLFGDSETSPFSIHNLLQAGRAYDLAAGSWVGP 240
                            PLDKEYVEILHLFGDSETS FSIHNLLQAGR YDLAAGSWVGP
Sbjct: 181 ALLFHRLGRSWRKTSQKPLDKEYVEILHLFGDSETSAFSIHNLLQAGRPYDLAAGSWVGP 240

Query: 241 YAMCRSWETLVRLKRETPNLQDQQLPMAIYIVSGDEDGERGGAPVLCIDDASRHCFEFSK 300
           YA+CRSWETLVRLKRETP  QDQQLPMAIYIVSGDEDGERGGAPVLCID ASRHCF+FSK
Sbjct: 241 YAICRSWETLVRLKRETPTPQDQQLPMAIYIVSGDEDGERGGAPVLCIDVASRHCFQFSK 300

Query: 301 GQLDWSPILLLVPLVLGLEKINPRYIPSLRTTFTFPQSLGILGGKPGASTYIVGVQDENA 354
           GQLDW+PILLLVPLVLGLEKINPRYIPSLR TFTFPQSLGILGGK GASTYIVGVQDENA
Sbjct: 301 GQLDWTPILLLVPLVLGLEKINPRYIPSLRATFTFPQSLGILGGKAGASTYIVGVQDENA 360

[Close]

BLAST of Lsi09G010710 vs. NCBI nr
Match: XP_004138950.1 (cysteine protease ATG4 [Cucumis sativus] >KGN61454.1 hypothetical protein Csa_006114 [Cucumis sativus])

HSP 1 Score: 651.0 bits (1678), Expect = 7.5e-183
Identity = 322/370 (87.03%), Postives = 330/370 (89.19%), Query Frame = 0


Query: 1   MGRGKDLNLTCSSESTTDIIDRTHQSVGPELGSKNHISSKASLWSGFFTSTFSIFEHHKE 60
           MGRGKDL  TCS E   D IDRTH+SV PELGSKNHISSKAS WSGFF+S FSIFEHHK+
Sbjct: 1   MGRGKDLKSTCSPEPAADAIDRTHRSVYPELGSKNHISSKASSWSGFFSSNFSIFEHHKD 60

Query: 61  SSVSEKKAFHSRHNVWATVRKVMTSGSMRRIQERIMGSRRSGVYSSGGDIWLLGVCHKIS 120
           SSV+EKK FH RHNVWATVRKVMTSGSMRRIQER++GSRRSGVYSSGGDIWLLGVCHKIS
Sbjct: 61  SSVTEKKVFHPRHNVWATVRKVMTSGSMRRIQERLLGSRRSGVYSSGGDIWLLGVCHKIS 120

Query: 121 QDQAPDDAVTSTGAAGYELDFSSRILMTYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQ 180
           QD  PDDA +S G AGYE DFSSRILMTYRKGF+VIQDSKYTSDVNWGCMLRSSQMLVAQ
Sbjct: 121 QDHPPDDAASSPGVAGYEQDFSSRILMTYRKGFHVIQDSKYTSDVNWGCMLRSSQMLVAQ 180

Query: 181 -----------------PLDKEYVEILHLFGDSETSPFSIHNLLQAGRAYDLAAGSWVGP 240
                            PLDKEYVEILHLFGDSETS FSIHNLLQAGRAYDLAAGSWVGP
Sbjct: 181 ALLFHRLGRSWRKPSQKPLDKEYVEILHLFGDSETSAFSIHNLLQAGRAYDLAAGSWVGP 240

Query: 241 YAMCRSWETLVRLKRETPNLQDQQLPMAIYIVSGDEDGERGGAPVLCIDDASRHCFEFSK 300
           YAMCRSWETLVR KRETP LQDQQLPMAIYIVSGDEDGERGGAPVL IDDASRHCFEFSK
Sbjct: 241 YAMCRSWETLVRSKRETPILQDQQLPMAIYIVSGDEDGERGGAPVLYIDDASRHCFEFSK 300

Query: 301 GQLDWSPILLLVPLVLGLEKINPRYIPSLRTTFTFPQSLGILGGKPGASTYIVGVQDENA 354
           GQ DWSPILLLVPLVLGLEKINPRYIPSLRTTFTFPQSLGILGGKPGASTYIVGVQDENA
Sbjct: 301 GQHDWSPILLLVPLVLGLEKINPRYIPSLRTTFTFPQSLGILGGKPGASTYIVGVQDENA 360

[Close]

BLAST of Lsi09G010710 vs. TAIR 10
Match: AT2G44140.1 (Peptidase family C54 protein )

HSP 1 Score: 410.2 bits (1053), Expect = 2.1e-114
Identity = 207/361 (57.34%), Postives = 265/361 (73.41%), Query Frame = 0


Query: 11  CSSESTTDIIDRTHQSVGPELGSKNHISSKASLWSGFFTSTFSIFEHHKESSVSEKKAFH 70
           CSS S +D  D++   +  + G  ++  SK +LWS  FTS+ S+ + ++ESS S  K   
Sbjct: 13  CSSSSKSDTHDKS--PLVSDSGPSDN-KSKFTLWSNVFTSSSSVSQPYRESSTSGHKQVC 72

Query: 71  SRHNVW-ATVRKV-MTSGSMRRIQERIMGSRRSGVYSSGGDIWLLGVCHKISQDQAPDDA 130
           +  N W A V++V M SG++RR QER++G  R+G+ S+  D+WLLGVC+KIS D+   + 
Sbjct: 73  TTRNGWTAFVKRVSMASGAIRRFQERVLGPNRTGLPSTTSDVWLLGVCYKISADENSGET 132

Query: 131 VTSTGAAGYELDFSSRILMTYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQPL------ 190
            T T  A  +LDFSS+ILMTYRKGF   +D+ YTSDVNWGCM+RSSQML AQ L      
Sbjct: 133 DTGTVLAALQLDFSSKILMTYRKGFEPFRDTTYTSDVNWGCMIRSSQMLFAQALLFHRLG 192

Query: 191 ----------DKEYVEILHLFGDSETSPFSIHNLLQAGRAYDLAAGSWVGPYAMCRSWET 250
                     ++EY+E L  FGDSE S FSIHNL+ AG +Y LAAGSWVGPYA+CR+WE+
Sbjct: 193 RAWTKKSELPEQEYLETLEPFGDSEPSAFSIHNLIIAGASYGLAAGSWVGPYAICRAWES 252

Query: 251 LVRLKRETPNLQDQQLPMAIYIVSGDEDGERGGAPVLCIDDASRHCFEFSKGQLDWSPIL 310
           L   KR+  + ++Q LPMA++IVSG EDGERGGAP+LCI+DA++ C EFSKGQ +W+PI+
Sbjct: 253 LACKKRKQTDSKNQTLPMAVHIVSGSEDGERGGAPILCIEDATKSCLEFSKGQSEWTPII 312

Query: 311 LLVPLVLGLEKINPRYIPSLRTTFTFPQSLGILGGKPGASTYIVGVQDENAFYLDPHEVQ 354
           LLVPLVLGL+ +NPRYIPSL  TFTFPQS+GILGGKPGASTYIVGVQ++  FYLDPHEVQ
Sbjct: 313 LLVPLVLGLDSVNPRYIPSLVATFTFPQSVGILGGKPGASTYIVGVQEDKGFYLDPHEVQ 370

[Close]

BLAST of Lsi09G010710 vs. TAIR 10
Match: AT2G44140.2 (Peptidase family C54 protein )

HSP 1 Score: 390.6 bits (1002), Expect = 1.7e-108
Identity = 191/313 (61.02%), Postives = 237/313 (75.72%), Query Frame = 0


Query: 59  KESSVSEKKAFHSRHNVW-ATVRKV-MTSGSMRRIQERIMGSRRSGVYSSGGDIWLLGVC 118
           +ESS S  K   +  N W A V++V M SG++RR QER++G  R+G+ S+  D+WLLGVC
Sbjct: 13  RESSTSGHKQVCTTRNGWTAFVKRVSMASGAIRRFQERVLGPNRTGLPSTTSDVWLLGVC 72

Query: 119 HKISQDQAPDDAVTSTGAAGYELDFSSRILMTYRKGFNVIQDSKYTSDVNWGCMLRSSQM 178
           +KIS D+   +  T T  A  +LDFSS+ILMTYRKGF   +D+ YTSDVNWGCM+RSSQM
Sbjct: 73  YKISADENSGETDTGTVLAALQLDFSSKILMTYRKGFEPFRDTTYTSDVNWGCMIRSSQM 132

Query: 179 LVAQPL----------------DKEYVEILHLFGDSETSPFSIHNLLQAGRAYDLAAGSW 238
           L AQ L                ++EY+E L  FGDSE S FSIHNL+ AG +Y LAAGSW
Sbjct: 133 LFAQALLFHRLGRAWTKKSELPEQEYLETLEPFGDSEPSAFSIHNLIIAGASYGLAAGSW 192

Query: 239 VGPYAMCRSWETLVRLKRETPNLQDQQLPMAIYIVSGDEDGERGGAPVLCIDDASRHCFE 298
           VGPYA+CR+WE+L   KR+  + ++Q LPMA++IVSG EDGERGGAP+LCI+DA++ C E
Sbjct: 193 VGPYAICRAWESLACKKRKQTDSKNQTLPMAVHIVSGSEDGERGGAPILCIEDATKSCLE 252

Query: 299 FSKGQLDWSPILLLVPLVLGLEKINPRYIPSLRTTFTFPQSLGILGGKPGASTYIVGVQD 354
           FSKGQ +W+PI+LLVPLVLGL+ +NPRYIPSL  TFTFPQS+GILGGKPGASTYIVGVQ+
Sbjct: 253 FSKGQSEWTPIILLVPLVLGLDSVNPRYIPSLVATFTFPQSVGILGGKPGASTYIVGVQE 312

[Close]

BLAST of Lsi09G010710 vs. TAIR 10
Match: AT3G59950.1 (Peptidase family C54 protein )

HSP 1 Score: 385.2 bits (988), Expect = 7.1e-107
Identity = 191/362 (52.76%), Postives = 250/362 (69.06%), Query Frame = 0


Query: 11  CSSESTTDIIDRTHQSVGPELGSKNHISSKASLWSGFFTSTFSIFEHHKESSVSEKKAFH 70
           CSS ST++  D +  +      + +   S  +L S    S+  + +  +E+S S      
Sbjct: 13  CSSSSTSEKRDISSPTSLVSDSASSDNKSNLTLCSDVVASSSPVSQLCREASTSGHNPVC 72

Query: 71  SRHNVWATVRKV--MTSGSMRRIQERIMGSRRSGVYSSGGDIWLLGVCHKISQDQAPDDA 130
           + H+ W  + K   M SG++RR Q+R++G  R+G+ SS  +IWLLGVC+KIS+ ++ ++A
Sbjct: 73  TTHSSWTVILKTASMASGAIRRFQDRVLGPSRTGISSSTSEIWLLGVCYKISEGESSEEA 132

Query: 131 VTSTGAAGYELDFSSRILMTYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQ-------- 190
                 A +  DFSS ILMTYR+GF  I D+ YTSDVNWGCMLRS QML AQ        
Sbjct: 133 DAGRVLAAFRQDFSSLILMTYRRGFEPIGDTTYTSDVNWGCMLRSGQMLFAQALLFQRLG 192

Query: 191 ---------PLDKEYVEILHLFGDSETSPFSIHNLLQAGRAYDLAAGSWVGPYAMCRSWE 250
                    P D++Y+EIL LFGD+E S FSIHNL+ AG +Y LAAGSWVGPYA+CRSWE
Sbjct: 193 RSWRKKDSEPADEKYLEILELFGDTEASAFSIHNLILAGESYGLAAGSWVGPYAVCRSWE 252

Query: 251 TLVRLKRETPNLQDQQLPMAIYIVSGDEDGERGGAPVLCIDDASRHCFEFSKGQLDWSPI 310
           +L R  +E  + + +   MA++IVSG EDGERGGAP+LCI+D ++ C EFS+G+ +W PI
Sbjct: 253 SLARKNKEETDDKHKSFSMAVHIVSGSEDGERGGAPILCIEDVTKTCLEFSEGETEWPPI 312

Query: 311 LLLVPLVLGLEKINPRYIPSLRTTFTFPQSLGILGGKPGASTYIVGVQDENAFYLDPHEV 354
           LLLVPLVLGL+++NPRYIPSL  TFTFPQSLGILGGKPGASTYIVGVQ++  FYLDPH+V
Sbjct: 313 LLLVPLVLGLDRVNPRYIPSLIATFTFPQSLGILGGKPGASTYIVGVQEDKGFYLDPHDV 372

[Close]

BLAST of Lsi09G010710 vs. TAIR 10
Match: AT3G59950.2 (Peptidase family C54 protein )

HSP 1 Score: 301.6 bits (771), Expect = 1.0e-81
Identity = 153/322 (47.52%), Postives = 211/322 (65.53%), Query Frame = 0


Query: 11  CSSESTTDIIDRTHQSVGPELGSKNHISSKASLWSGFFTSTFSIFEHHKESSVSEKKAFH 70
           CSS ST++  D +  +      + +   S  +L S    S+  + +  +E+S S      
Sbjct: 13  CSSSSTSEKRDISSPTSLVSDSASSDNKSNLTLCSDVVASSSPVSQLCREASTSGHNPVC 72

Query: 71  SRHNVWATVRKV--MTSGSMRRIQERIMGSRRSGVYSSGGDIWLLGVCHKISQDQAPDDA 130
           + H+ W  + K   M SG++RR Q+R++G  R+G+ SS  +IWLLGVC+KIS+ ++ ++A
Sbjct: 73  TTHSSWTVILKTASMASGAIRRFQDRVLGPSRTGISSSTSEIWLLGVCYKISEGESSEEA 132

Query: 131 VTSTGAAGYELDFSSRILMTYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQ-------- 190
                 A +  DFSS ILMTYR+GF  I D+ YTSDVNWGCMLRS QML AQ        
Sbjct: 133 DAGRVLAAFRQDFSSLILMTYRRGFEPIGDTTYTSDVNWGCMLRSGQMLFAQALLFQRLG 192

Query: 191 ---------PLDKEYVEILHLFGDSETSPFSIHNLLQAGRAYDLAAGSWVGPYAMCRSWE 250
                    P D++Y+EIL LFGD+E S FSIHNL+ AG +Y LAAGSWVGPYA+CRSWE
Sbjct: 193 RSWRKKDSEPADEKYLEILELFGDTEASAFSIHNLILAGESYGLAAGSWVGPYAVCRSWE 252

Query: 251 TLVRLKRETPNLQDQQLPMAIYIVSGDEDGERGGAPVLCIDDASRHCFEFSKGQLDWSPI 310
           +L R  +E  + + +   MA++IVSG EDGERGGAP+LCI+D ++ C EFS+G+ +W PI
Sbjct: 253 SLARKNKEETDDKHKSFSMAVHIVSGSEDGERGGAPILCIEDVTKTCLEFSEGETEWPPI 312

Query: 311 LLLVPLVLGLEKINPR--YIPS 312
           LLLVPLVLGL+++NP   ++PS
Sbjct: 313 LLLVPLVLGLDRVNPSHFHVPS 334

[Close]

BLAST of Lsi09G010710 vs. TAIR 10
Match: AT3G59950.3 (Peptidase family C54 protein )

HSP 1 Score: 294.3 bits (752), Expect = 1.7e-79
Identity = 136/244 (55.74%), Postives = 179/244 (73.36%), Query Frame = 0


Query: 83  MTSGSMRRIQERIMGSRRSGVYSSGGDIWLLGVCHKISQDQAPDDAVTSTGAAGYELDFS 142
           M SG++RR Q+R++G  R+G+ SS  +IWLLGVC+KIS+ ++ ++A      A +  DFS
Sbjct: 1   MASGAIRRFQDRVLGPSRTGISSSTSEIWLLGVCYKISEGESSEEADAGRVLAAFRQDFS 60

Query: 143 SRILMTYRKGFNVIQDSKYTSDVNWGCMLRSSQMLVAQ-----------------PLDKE 202
           S ILMTYR+GF  I D+ YTSDVNWGCMLRS QML AQ                 P D++
Sbjct: 61  SLILMTYRRGFEPIGDTTYTSDVNWGCMLRSGQMLFAQALLFQRLGRSWRKKDSEPADEK 120

Query: 203 YVEILHLFGDSETSPFSIHNLLQAGRAYDLAAGSWVGPYAMCRSWETLVRLKRETPNLQD 262
           Y+EIL LFGD+E S FSIHNL+ AG +Y LAAGSWVGPYA+CRSWE+L R  +E  + + 
Sbjct: 121 YLEILELFGDTEASAFSIHNLILAGESYGLAAGSWVGPYAVCRSWESLARKNKEETDDKH 180

Query: 263 QQLPMAIYIVSGDEDGERGGAPVLCIDDASRHCFEFSKGQLDWSPILLLVPLVLGLEKIN 310
           +   MA++IVSG EDGERGGAP+LCI+D ++ C EFS+G+ +W PILLLVPLVLGL+++N
Sbjct: 181 KSFSMAVHIVSGSEDGERGGAPILCIEDVTKTCLEFSEGETEWPPILLLVPLVLGLDRVN 240

The following BLAST results are available for this feature:

BLAST of Lsi09G010710 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A2Q1V6	4.5e-130	63.89	Cysteine protease ATG4 OS=Medicago truncatula OX=3880 GN=ATG4 PE=3 SV=1	[more]
Q8S929	2.9e-113	57.34	Cysteine protease ATG4a OS=Arabidopsis thaliana OX=3702 GN=ATG4A PE=2 SV=1	[more]
Q9M1Y0	1.0e-105	52.76	Cysteine protease ATG4b OS=Arabidopsis thaliana OX=3702 GN=ATG4B PE=1 SV=1	[more]
Q7XPW8	2.2e-105	57.27	Cysteine protease ATG4B OS=Oryza sativa subsp. japonica OX=39947 GN=ATG4B PE=2 S...	[more]
Q2XPP4	5.0e-105	56.69	Cysteine protease ATG4B OS=Oryza sativa subsp. indica OX=39946 GN=ATG4B PE=1 SV=...	[more]

BLAST of Lsi09G010710 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7SP82	2.5e-184	87.30	Cysteine protease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold340G00...	[more]
A0A1S3B4R1	2.5e-184	87.30	Cysteine protease OS=Cucumis melo OX=3656 GN=LOC103485762 PE=3 SV=1	[more]
A0A6J1ETR9	1.2e-183	87.03	Cysteine protease OS=Cucurbita moschata OX=3662 GN=LOC111436439 PE=3 SV=1	[more]
A0A0A0LK81	3.6e-183	87.03	Cysteine protease OS=Cucumis sativus OX=3659 GN=Csa_2G128640 PE=3 SV=1	[more]
A0A6J1ICU1	4.7e-183	86.49	Cysteine protease OS=Cucurbita maxima OX=3661 GN=LOC111472623 PE=3 SV=1	[more]

BLAST of Lsi09G010710 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038874853.1	1.6e-196	92.70	cysteine protease ATG4-like [Benincasa hispida]	[more]
XP_008441684.1	5.2e-184	87.30	PREDICTED: cysteine protease ATG4-like isoform X1 [Cucumis melo] >KAA0032358.1 c...	[more]
XP_023539406.1	6.8e-184	87.03	cysteine protease ATG4-like [Cucurbita pepo subsp. pepo] >XP_023539414.1 cystein...	[more]
XP_022929983.1	2.6e-183	87.03	cysteine protease ATG4-like [Cucurbita moschata] >XP_022929984.1 cysteine protea...	[more]
XP_004138950.1	7.5e-183	87.03	cysteine protease ATG4 [Cucumis sativus] >KGN61454.1 hypothetical protein Csa_00...	[more]

BLAST of Lsi09G010710 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G44140.1	2.1e-114	57.34	Peptidase family C54 protein	[more]
AT2G44140.2	1.7e-108	61.02	Peptidase family C54 protein	[more]
AT3G59950.1	7.1e-107	52.76	Peptidase family C54 protein	[more]
AT3G59950.2	1.0e-81	47.52	Peptidase family C54 protein	[more]
AT3G59950.3	1.7e-79	55.74	Peptidase family C54 protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005078	Peptidase C54	PFAM	PF03416	Peptidase_C54	coord: 139..355 e-value: 7.6E-58 score: 196.1
IPR005078	Peptidase C54	PANTHER	PTHR22624	CYSTEINE PROTEASE ATG4	coord: 56..354
None	No IPR available	PANTHER	PTHR22624:SF55	CYSTEINE PROTEASE	coord: 56..354
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	54001	Cysteine proteinases	coord: 95..356

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi09G010710.1	Lsi09G010710.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006914	autophagy
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0008234	cysteine-type peptidase activity