Lsi09G006180 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi09G006180
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionCore-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family protein
Locationchr09: 6809593 .. 6811662 (+)
RNA-Seq ExpressionLsi09G006180
SyntenyLsi09G006180
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGAATTAAAACCAAACTTCCCCTTCTCCCACATCGTCTGCCCTCCGCCACCCTCTCCGACGTAGCCTCACCTCCGATCAAGCGCCATCCGGCCAATTTCCGATCTGGGTATTCGAAAATTTGGCAAAAAATGAAGAGTCAAATTCAGAGTCCGAAGCTAATCCATATCCATCTTAGCTTCTTCAACGTCGTCCCTTACATCCTCCTCTTCACCGCCGGAATCACCGCCGGTGCTTTCCTCACTTTCTATCTCTCCAATTTCTCCATAAGCTTAAACCTCACCCAAATCCCCTCTTCGAGTTTTTTTCCAGTGACCGGTGGCCGGGTGGGGTTGGAAGAGTATTTGAAGCCGCCGGAAGTAATGCACGACATGGAGGACGAGGAGCTTCTGTGGAGATCTTCAATGACGGCGGGAATTAAGAAATTTCCATTCCGGCGGGTTCCAAAGATAGCGTTCATGTTCTTGACGAAAGGGCCTGTGTATTTGGCTCCTCTTTGGGAAGAATTCTTTAAGGGAAATGAAGGGCTTTACTCTGTTTATGTTCATTCTGATCCTTCTTATAATCATTCTTTCCCTGAATCCCCTGTTTTCCATGGCCGGAGAATTCCCAGCAAGGTTAGTATTCTTATAATCAACTATCATATAAGAATCAATTAAATAAAGAACAAGCTGGGCTAAATTTCAGTTTCAAAAGTAACTCTTATTTTTAAAAATTTCAATATTATCCTTCAACTTTCATAAATGTTTCAAAACTATTCTTAAAGTATAAATTCCTTTTCAATTTTGAACAAAAATTACTTAGAATAATGTGTTGATTTAAATTTTAGTTCTATGTCACATACTACACCATTTCATATCTTAATTTCCATCCAAACTATAAAGAATTTCTACTTAAAGGATATTTTTGAAATGTTTATAAAAGTTCAATGGTAATATTGAAACTTTTGAAAGTATGAGAGTATTTTTTAAACAAAGAATAAAGTTCAAAGGTATTTTTATAATTTAATTAATTTTCTTTTAAAAAAAATCATTTAGAAAATATTTGTCACATGACAATTTTTTATTAGATAGCTAGTTGTATAAGCTGTATAAAGATAAGTGCAATGTAGATAACACCTAAGTTTTTCTCTAAATAAAAATTAGGAAATATATCAATTTATACCCTAAACTAACCTTGTGTAAATTTAGACAACCTCATGTAGATTTAGGGTTTTAATTAATACAACCCCAAAGTTAATTGATATTTTTCATAAAAATTAATAAAACCTATTGATTTTTTTTTTTTTTTTTTTTGGGTTTTGATATTTATTAATGTGCATTACAGAAAGTGGGATGGGGAAAGGTAAACATGATCGAGGCAGAACGTCGCCTATTATCAAACGCACTCCTTGACATTTCAAACGAGCGATTTATTCTCCTTTCAGAAGCTTGTATTCCTCTCTTCAACTTCTCCACAGTCTACTCCTTCCTCATCAACTCCACCATGAAAAGCTTCATCATGAGCTACGACGAACCCGGTAATGTCGGTCGCGGCCGATACAGGAACAAGATGTTCCCACCCATCTCCCTAAAACAATGGCGTAAAGGCTCACAGTGGTTCGAAATGGACCGAGAAACCGCCGTTGCCGTCGTCTCCGACATGAAGTACTTTCCTGTCTTTCAGAATTACTGCAAAGGCCAATGTTACTCCGATGAACATTACTTGCCGACCTTGGTCAATATTTTGGGCTGGAACAGGAATGCCAATCGAAGTTTGACTTGGGTTGATTGGTCAAAGGGTGGCCCACATCCTACGAGATTCTCGTGGTCAGATATAAACGTGGAGCTTTTTCAAAGATTAAGGAATCAAACCCATAAATGTATAAAAACTAAATTGGAAGGAACAGGTGTTTGCTTTTTATTCGCCAGAAAATTTGCTCCAAACACATTGGGGAGGCTGATGAAGATTGCACCAAAGGCCATGCACTTTGGGAGATAGATAGATTAGAGCAATTGAAATTTATTTGTTCATTTATTTATTTAGAATTAGGTTATATATATTTATACGTATAAATTTTGATTTTTTAGTAA

mRNA sequence

GGAATTAAAACCAAACTTCCCCTTCTCCCACATCGTCTGCCCTCCGCCACCCTCTCCGACGTAGCCTCACCTCCGATCAAGCGCCATCCGGCCAATTTCCGATCTGGGTATTCGAAAATTTGGCAAAAAATGAAGAGTCAAATTCAGAGTCCGAAGCTAATCCATATCCATCTTAGCTTCTTCAACGTCGTCCCTTACATCCTCCTCTTCACCGCCGGAATCACCGCCGGTGCTTTCCTCACTTTCTATCTCTCCAATTTCTCCATAAGCTTAAACCTCACCCAAATCCCCTCTTCGAGTTTTTTTCCAGTGACCGGTGGCCGGGTGGGGTTGGAAGAGTATTTGAAGCCGCCGGAAGTAATGCACGACATGGAGGACGAGGAGCTTCTGTGGAGATCTTCAATGACGGCGGGAATTAAGAAATTTCCATTCCGGCGGGTTCCAAAGATAGCGTTCATGTTCTTGACGAAAGGGCCTGTGTATTTGGCTCCTCTTTGGGAAGAATTCTTTAAGGGAAATGAAGGGCTTTACTCTGTTTATGTTCATTCTGATCCTTCTTATAATCATTCTTTCCCTGAATCCCCTGTTTTCCATGGCCGGAGAATTCCCAGCAAGAAAGTGGGATGGGGAAAGGTAAACATGATCGAGGCAGAACGTCGCCTATTATCAAACGCACTCCTTGACATTTCAAACGAGCGATTTATTCTCCTTTCAGAAGCTTGTATTCCTCTCTTCAACTTCTCCACAGTCTACTCCTTCCTCATCAACTCCACCATGAAAAGCTTCATCATGAGCTACGACGAACCCGGTAATGTCGGTCGCGGCCGATACAGGAACAAGATGTTCCCACCCATCTCCCTAAAACAATGGCGTAAAGGCTCACAGTGGTTCGAAATGGACCGAGAAACCGCCGTTGCCGTCGTCTCCGACATGAAGTACTTTCCTGTCTTTCAGAATTACTGCAAAGGCCAATGTTACTCCGATGAACATTACTTGCCGACCTTGGTCAATATTTTGGGCTGGAACAGGAATGCCAATCGAAGTTTGACTTGGGTTGATTGGTCAAAGGGTGGCCCACATCCTACGAGATTCTCGTGGTCAGATATAAACGTGGAGCTTTTTCAAAGATTAAGGAATCAAACCCATAAATGTATAAAAACTAAATTGGAAGGAACAGGTGTTTGCTTTTTATTCGCCAGAAAATTTGCTCCAAACACATTGGGGAGGCTGATGAAGATTGCACCAAAGGCCATGCACTTTGGGAGATAGATAGATTAGAGCAATTGAAATTTATTTGTTCATTTATTTATTTAGAATTAGGTTATATATATTTATACGTATAAATTTTGATTTTTTAGTAA

Coding sequence (CDS)

GGAATTAAAACCAAACTTCCCCTTCTCCCACATCGTCTGCCCTCCGCCACCCTCTCCGACGTAGCCTCACCTCCGATCAAGCGCCATCCGGCCAATTTCCGATCTGGGTATTCGAAAATTTGGCAAAAAATGAAGAGTCAAATTCAGAGTCCGAAGCTAATCCATATCCATCTTAGCTTCTTCAACGTCGTCCCTTACATCCTCCTCTTCACCGCCGGAATCACCGCCGGTGCTTTCCTCACTTTCTATCTCTCCAATTTCTCCATAAGCTTAAACCTCACCCAAATCCCCTCTTCGAGTTTTTTTCCAGTGACCGGTGGCCGGGTGGGGTTGGAAGAGTATTTGAAGCCGCCGGAAGTAATGCACGACATGGAGGACGAGGAGCTTCTGTGGAGATCTTCAATGACGGCGGGAATTAAGAAATTTCCATTCCGGCGGGTTCCAAAGATAGCGTTCATGTTCTTGACGAAAGGGCCTGTGTATTTGGCTCCTCTTTGGGAAGAATTCTTTAAGGGAAATGAAGGGCTTTACTCTGTTTATGTTCATTCTGATCCTTCTTATAATCATTCTTTCCCTGAATCCCCTGTTTTCCATGGCCGGAGAATTCCCAGCAAGAAAGTGGGATGGGGAAAGGTAAACATGATCGAGGCAGAACGTCGCCTATTATCAAACGCACTCCTTGACATTTCAAACGAGCGATTTATTCTCCTTTCAGAAGCTTGTATTCCTCTCTTCAACTTCTCCACAGTCTACTCCTTCCTCATCAACTCCACCATGAAAAGCTTCATCATGAGCTACGACGAACCCGGTAATGTCGGTCGCGGCCGATACAGGAACAAGATGTTCCCACCCATCTCCCTAAAACAATGGCGTAAAGGCTCACAGTGGTTCGAAATGGACCGAGAAACCGCCGTTGCCGTCGTCTCCGACATGAAGTACTTTCCTGTCTTTCAGAATTACTGCAAAGGCCAATGTTACTCCGATGAACATTACTTGCCGACCTTGGTCAATATTTTGGGCTGGAACAGGAATGCCAATCGAAGTTTGACTTGGGTTGATTGGTCAAAGGGTGGCCCACATCCTACGAGATTCTCGTGGTCAGATATAAACGTGGAGCTTTTTCAAAGATTAAGGAATCAAACCCATAAATGTATAAAAACTAAATTGGAAGGAACAGGTGTTTGCTTTTTATTCGCCAGAAAATTTGCTCCAAACACATTGGGGAGGCTGATGAAGATTGCACCAAAGGCCATGCACTTTGGGAGATAG

Protein sequence

GIKTKLPLLPHRLPSATLSDVASPPIKRHPANFRSGYSKIWQKMKSQIQSPKLIHIHLSFFNVVPYILLFTAGITAGAFLTFYLSNFSISLNLTQIPSSSFFPVTGGRVGLEEYLKPPEVMHDMEDEELLWRSSMTAGIKKFPFRRVPKIAFMFLTKGPVYLAPLWEEFFKGNEGLYSVYVHSDPSYNHSFPESPVFHGRRIPSKKVGWGKVNMIEAERRLLSNALLDISNERFILLSEACIPLFNFSTVYSFLINSTMKSFIMSYDEPGNVGRGRYRNKMFPPISLKQWRKGSQWFEMDRETAVAVVSDMKYFPVFQNYCKGQCYSDEHYLPTLVNILGWNRNANRSLTWVDWSKGGPHPTRFSWSDINVELFQRLRNQTHKCIKTKLEGTGVCFLFARKFAPNTLGRLMKIAPKAMHFGR
Homology
BLAST of Lsi09G006180 vs. ExPASy Swiss-Prot
Match: Q65XS5 (Glycosyltransferase BC10 OS=Oryza sativa subsp. japonica OX=39947 GN=BC10 PE=1 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 1.2e-36
Identity = 98/297 (33.00%), Postives = 155/297 (52.19%), Query Frame = 0

Query: 149 KIAFMFLTKGPVYLAPLWEEFFKGN-EGLYSVYVHSDPSY--NHSFPESPVFHGRRI-PS 208
           ++AF+F+ +  + L  +W+ FF+G+ EG +S++VHS P +    +   S  F+ R++  S
Sbjct: 85  RLAFLFIARNRLPLDLVWDAFFRGDKEGRFSIFVHSRPGFVLTRATTRSGFFYNRQVNNS 144

Query: 209 KKVGWGKVNMIEAERRLLSNALLDISNERFILLSEACIPLFNFSTVYSFLINSTMKSFIM 268
            +V WG+ +MIEAER LL++AL D  NERF+ +S++C+PL+NF+  Y ++++S+  SF+ 
Sbjct: 145 VQVDWGEASMIEAERVLLAHALKDPLNERFVFVSDSCVPLYNFNYTYDYIMSSS-TSFVD 204

Query: 269 SYDEPGNVGRGRYRNKMFPPISLKQWRKGSQWFEMDRETAVAVVSDMKYFPVFQNYCKGQ 328
           S+    +   GRY  +M P I ++ WRKGSQW  + R+ A  VV D +  P FQ +C+ +
Sbjct: 205 SF---ADTKAGRYNPRMDPIIPVENWRKGSQWAVLTRKHAEVVVEDEEVLPEFQKHCRRR 264

Query: 329 ----------------------CYSDEHYLPTLVNILGWNRN-ANRSLTWVDW------- 388
                                 C  DEHY+ TL+   G       RS+T   W       
Sbjct: 265 PLPEFWRDWDRPIPAEAWKAHNCIPDEHYVQTLLAQHGLEEELTRRSVTHSAWDLSSSKD 324

Query: 389 -SKGGPHPTRFSWSDINVELFQRLRN--------QTHKCIKTKLEGTGVCFLFARKF 403
             + G HP  +  SD    L + +++        +  K   T       CFLFARKF
Sbjct: 325 RERRGWHPVTYKISDATPALVKSIKDIDNIYYETENRKEWCTSNGKPAPCFLFARKF 377

BLAST of Lsi09G006180 vs. ExPASy TrEMBL
Match: A0A5D3BMR2 (Core-2/I-branching enzyme OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1728G00110 PE=4 SV=1)

HSP 1 Score: 734.2 bits (1894), Expect = 3.1e-208
Identity = 349/379 (92.08%), Postives = 366/379 (96.57%), Query Frame = 0

Query: 44  MKSQIQSPKLIHIHLSFFNVVPYILLFTAGITAGAFLTFYLSNFSISLNLTQIPSSSFFP 103
           MKSQ QSPKLIHIHL+FFNVVPYILLFT GI+AG FLTFYLSNFSISLNLTQIPSS FFP
Sbjct: 1   MKSQFQSPKLIHIHLTFFNVVPYILLFTVGISAGVFLTFYLSNFSISLNLTQIPSSDFFP 60

Query: 104 VTGGRVGLEEYLKPPEVMHDMEDEELLWRSSMTAGIKKFPFRRVPKIAFMFLTKGPVYLA 163
           VTGGRVGLEE+LKPPEVMHDM+DEELLWR+SMTAGIKKFPF+RVPKIAFMFLTKGPVYLA
Sbjct: 61  VTGGRVGLEEFLKPPEVMHDMDDEELLWRASMTAGIKKFPFQRVPKIAFMFLTKGPVYLA 120

Query: 164 PLWEEFFKGNEGLYSVYVHSDPSYNHSFPESPVFHGRRIPSKKVGWGKVNMIEAERRLLS 223
           PLWEEFFKGNE LYSVYVHSDPSYNHS PESPVFHGRRIPSKKVGWGKVNMIEAERRL+S
Sbjct: 121 PLWEEFFKGNERLYSVYVHSDPSYNHSSPESPVFHGRRIPSKKVGWGKVNMIEAERRLIS 180

Query: 224 NALLDISNERFILLSEACIPLFNFSTVYSFLINSTMKSFIMSYDEPGNVGRGRYRNKMFP 283
           NALLDISNERF+LLSE+CIPLFNFSTVYSFLINSTMKSFIMSYDEPGNVGRGRYRNKMFP
Sbjct: 181 NALLDISNERFVLLSESCIPLFNFSTVYSFLINSTMKSFIMSYDEPGNVGRGRYRNKMFP 240

Query: 284 PISLKQWRKGSQWFEMDRETAVAVVSDMKYFPVFQNYCKGQCYSDEHYLPTLVNILGWNR 343
           PISLKQWRKGSQWFEMDR+TAVAVVSD KYFPVFQNYCKGQCYSDEHYLPTLVN+LGW+R
Sbjct: 241 PISLKQWRKGSQWFEMDRDTAVAVVSDKKYFPVFQNYCKGQCYSDEHYLPTLVNVLGWDR 300

Query: 344 NANRSLTWVDWSKGGPHPTRFSWSDINVELFQRLRNQTHKCIKTKLEGTGVCFLFARKFA 403
           NANRSLTWVDWSKGGPHP RFS SDI+VEL QRLRNQT +C K+K+EGTGVCFLFARKFA
Sbjct: 301 NANRSLTWVDWSKGGPHPARFSRSDIHVELIQRLRNQTGECRKSKMEGTGVCFLFARKFA 360

Query: 404 PNTLGRLMKIAPKAMHFGR 423
           PNTL RLMKIAPKAM+FGR
Sbjct: 361 PNTLERLMKIAPKAMYFGR 379

BLAST of Lsi09G006180 vs. ExPASy TrEMBL
Match: A0A1S3CU90 (uncharacterized protein LOC103504601 OS=Cucumis melo OX=3656 GN=LOC103504601 PE=4 SV=1)

HSP 1 Score: 734.2 bits (1894), Expect = 3.1e-208
Identity = 349/379 (92.08%), Postives = 366/379 (96.57%), Query Frame = 0

Query: 44  MKSQIQSPKLIHIHLSFFNVVPYILLFTAGITAGAFLTFYLSNFSISLNLTQIPSSSFFP 103
           MKSQ QSPKLIHIHL+FFNVVPYILLFT GI+AG FLTFYLSNFSISLNLTQIPSS FFP
Sbjct: 1   MKSQFQSPKLIHIHLTFFNVVPYILLFTVGISAGVFLTFYLSNFSISLNLTQIPSSDFFP 60

Query: 104 VTGGRVGLEEYLKPPEVMHDMEDEELLWRSSMTAGIKKFPFRRVPKIAFMFLTKGPVYLA 163
           VTGGRVGLEE+LKPPEVMHDM+DEELLWR+SMTAGIKKFPF+RVPKIAFMFLTKGPVYLA
Sbjct: 61  VTGGRVGLEEFLKPPEVMHDMDDEELLWRASMTAGIKKFPFQRVPKIAFMFLTKGPVYLA 120

Query: 164 PLWEEFFKGNEGLYSVYVHSDPSYNHSFPESPVFHGRRIPSKKVGWGKVNMIEAERRLLS 223
           PLWEEFFKGNE LYSVYVHSDPSYNHS PESPVFHGRRIPSKKVGWGKVNMIEAERRL+S
Sbjct: 121 PLWEEFFKGNERLYSVYVHSDPSYNHSSPESPVFHGRRIPSKKVGWGKVNMIEAERRLIS 180

Query: 224 NALLDISNERFILLSEACIPLFNFSTVYSFLINSTMKSFIMSYDEPGNVGRGRYRNKMFP 283
           NALLDISNERF+LLSE+CIPLFNFSTVYSFLINSTMKSFIMSYDEPGNVGRGRYRNKMFP
Sbjct: 181 NALLDISNERFVLLSESCIPLFNFSTVYSFLINSTMKSFIMSYDEPGNVGRGRYRNKMFP 240

Query: 284 PISLKQWRKGSQWFEMDRETAVAVVSDMKYFPVFQNYCKGQCYSDEHYLPTLVNILGWNR 343
           PISLKQWRKGSQWFEMDR+TAVAVVSD KYFPVFQNYCKGQCYSDEHYLPTLVN+LGW+R
Sbjct: 241 PISLKQWRKGSQWFEMDRDTAVAVVSDKKYFPVFQNYCKGQCYSDEHYLPTLVNVLGWDR 300

Query: 344 NANRSLTWVDWSKGGPHPTRFSWSDINVELFQRLRNQTHKCIKTKLEGTGVCFLFARKFA 403
           NANRSLTWVDWSKGGPHP RFS SDI+VEL QRLRNQT +C K+K+EGTGVCFLFARKFA
Sbjct: 301 NANRSLTWVDWSKGGPHPARFSRSDIHVELIQRLRNQTGECRKSKMEGTGVCFLFARKFA 360

Query: 404 PNTLGRLMKIAPKAMHFGR 423
           PNTL RLMKIAPKAM+FGR
Sbjct: 361 PNTLERLMKIAPKAMYFGR 379

BLAST of Lsi09G006180 vs. ExPASy TrEMBL
Match: A0A6J1FLQ9 (uncharacterized protein LOC111445363 OS=Cucurbita moschata OX=3662 GN=LOC111445363 PE=4 SV=1)

HSP 1 Score: 716.5 bits (1848), Expect = 6.7e-203
Identity = 340/379 (89.71%), Postives = 362/379 (95.51%), Query Frame = 0

Query: 44  MKSQIQSPKLIHIHLSFFNVVPYILLFTAGITAGAFLTFYLSNFSISLNLTQIPSSSFFP 103
           MKSQIQSPKLIHIHLSFFNVVPYILLFTAGITAG FLTFYLSNFSI+LNLTQIP S   P
Sbjct: 1   MKSQIQSPKLIHIHLSFFNVVPYILLFTAGITAGVFLTFYLSNFSINLNLTQIPPSISSP 60

Query: 104 VTGGRVGLEEYLKPPEVMHDMEDEELLWRSSMTAGIKKFPFRRVPKIAFMFLTKGPVYLA 163
           VTG RVGLEEYLKPPEVMHDMED+ELLWR+SM AGI++FPFRRVPK+AFMFLT+GP+YLA
Sbjct: 61  VTGPRVGLEEYLKPPEVMHDMEDQELLWRASMAAGIREFPFRRVPKVAFMFLTRGPMYLA 120

Query: 164 PLWEEFFKGNEGLYSVYVHSDPSYNHSFPESPVFHGRRIPSKKVGWGKVNMIEAERRLLS 223
           PLWEEFFKGNEGLYSVY+HSDPSYNHSFPESPVFHGRRIPSK+VGWGKVNMIEAERRL+S
Sbjct: 121 PLWEEFFKGNEGLYSVYIHSDPSYNHSFPESPVFHGRRIPSKQVGWGKVNMIEAERRLIS 180

Query: 224 NALLDISNERFILLSEACIPLFNFSTVYSFLINSTMKSFIMSYDEPGNVGRGRYRNKMFP 283
           NALLDISNERF+LLSEACIPLFNFSTVYSFL+NSTMKSFIMSYDEP NVGRGRYR KMFP
Sbjct: 181 NALLDISNERFVLLSEACIPLFNFSTVYSFLMNSTMKSFIMSYDEPSNVGRGRYRAKMFP 240

Query: 284 PISLKQWRKGSQWFEMDRETAVAVVSDMKYFPVFQNYCKGQCYSDEHYLPTLVNILGWNR 343
           PISLKQWRKGSQWFEMDR+TAVAVVSD KYFPVF  YCKGQCYSDEHY+PTLVN+LGW+R
Sbjct: 241 PISLKQWRKGSQWFEMDRDTAVAVVSDRKYFPVFVKYCKGQCYSDEHYMPTLVNVLGWDR 300

Query: 344 NANRSLTWVDWSKGGPHPTRFSWSDINVELFQRLRNQTHKCIKTKLEGTGVCFLFARKFA 403
           NANRSLTWVDWSKGGPHPTRFS SDI+VEL QRLRNQT +C K+K EGTGVCFLFARKF+
Sbjct: 301 NANRSLTWVDWSKGGPHPTRFSRSDIHVELLQRLRNQT-ECTKSKTEGTGVCFLFARKFS 360

Query: 404 PNTLGRLMKIAPKAMHFGR 423
           PNTLGRLMKIAPKA+HFGR
Sbjct: 361 PNTLGRLMKIAPKALHFGR 378

BLAST of Lsi09G006180 vs. ExPASy TrEMBL
Match: A0A0A0KT17 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G576840 PE=4 SV=1)

HSP 1 Score: 708.4 bits (1827), Expect = 1.8e-200
Identity = 336/379 (88.65%), Postives = 356/379 (93.93%), Query Frame = 0

Query: 44  MKSQIQSPKLIHIHLSFFNVVPYILLFTAGITAGAFLTFYLSNFSISLNLTQIPSSSFFP 103
           MKSQ QSPKLIHIHL+FF+VVPYILLFT GITAG FLTFYLSNF ISLNLTQI SS FFP
Sbjct: 1   MKSQFQSPKLIHIHLTFFHVVPYILLFTVGITAGVFLTFYLSNFFISLNLTQISSSDFFP 60

Query: 104 VTGGRVGLEEYLKPPEVMHDMEDEELLWRSSMTAGIKKFPFRRVPKIAFMFLTKGPVYLA 163
           VTGGRVGLEE+LKPPEVMHDM+DEELLWR+SM A IKKFPF+RVPKIAFMFLTKGPVYLA
Sbjct: 61  VTGGRVGLEEFLKPPEVMHDMDDEELLWRASMMARIKKFPFQRVPKIAFMFLTKGPVYLA 120

Query: 164 PLWEEFFKGNEGLYSVYVHSDPSYNHSFPESPVFHGRRIPSKKVGWGKVNMIEAERRLLS 223
           PLWEEFFKGNEGLYSVYVHSDPSYNHS PE P FHGRRIPSKKVGWGKVNMIEAERRL+S
Sbjct: 121 PLWEEFFKGNEGLYSVYVHSDPSYNHSSPEPPAFHGRRIPSKKVGWGKVNMIEAERRLIS 180

Query: 224 NALLDISNERFILLSEACIPLFNFSTVYSFLINSTMKSFIMSYDEPGNVGRGRYRNKMFP 283
           NALLDISNERF+LLSE+CIPLFNFSTVYSFLINSTMKSFIMSYDEP NVGRGRYRNKMFP
Sbjct: 181 NALLDISNERFVLLSESCIPLFNFSTVYSFLINSTMKSFIMSYDEPSNVGRGRYRNKMFP 240

Query: 284 PISLKQWRKGSQWFEMDRETAVAVVSDMKYFPVFQNYCKGQCYSDEHYLPTLVNILGWNR 343
           PISLKQWRKGSQWFE+DR+TAVAVVSD KYFPVFQNYCKGQCYSDEHYLPTLVN+LGW+R
Sbjct: 241 PISLKQWRKGSQWFEIDRDTAVAVVSDKKYFPVFQNYCKGQCYSDEHYLPTLVNVLGWDR 300

Query: 344 NANRSLTWVDWSKGGPHPTRFSWSDINVELFQRLRNQTHKCIKTKLEGTGVCFLFARKFA 403
           N NRSLTWVDWSKGGPHP R+S SDI+VEL QRLRNQT +C K+K+EG GVCFLFARKFA
Sbjct: 301 NGNRSLTWVDWSKGGPHPARYSRSDIHVELIQRLRNQTGECRKSKMEGKGVCFLFARKFA 360

Query: 404 PNTLGRLMKIAPKAMHFGR 423
           PN L RL+ IAPKAM+FGR
Sbjct: 361 PNALERLVNIAPKAMYFGR 379

BLAST of Lsi09G006180 vs. ExPASy TrEMBL
Match: A0A6J1K052 (uncharacterized protein LOC111489841 OS=Cucurbita maxima OX=3661 GN=LOC111489841 PE=4 SV=1)

HSP 1 Score: 691.0 bits (1782), Expect = 3.0e-195
Identity = 330/379 (87.07%), Postives = 355/379 (93.67%), Query Frame = 0

Query: 44  MKSQIQSPKLIHIHLSFFNVVPYILLFTAGITAGAFLTFYLSNFSISLNLTQIPSSSFFP 103
           MK+QIQSPKLIHIHLSFFNVVPYILLFTAG+    FLTFYLSNFSISLNLTQIP S   P
Sbjct: 1   MKNQIQSPKLIHIHLSFFNVVPYILLFTAGV----FLTFYLSNFSISLNLTQIPPSISSP 60

Query: 104 VTGGRVGLEEYLKPPEVMHDMEDEELLWRSSMTAGIKKFPFRRVPKIAFMFLTKGPVYLA 163
           VTG RVGLEEYLKPPEVMHDMEDEELLWR+SM AGI++FPFRRVPK+AFMFLT+GP+YLA
Sbjct: 61  VTGPRVGLEEYLKPPEVMHDMEDEELLWRASMAAGIREFPFRRVPKVAFMFLTRGPMYLA 120

Query: 164 PLWEEFFKGNEGLYSVYVHSDPSYNHSFPESPVFHGRRIPSKKVGWGKVNMIEAERRLLS 223
           PLW EFFKGNEGLYSVY+HS+PSYNHSF ESPVFHGRRIPSK+V WG VNMIEAERRL+S
Sbjct: 121 PLWGEFFKGNEGLYSVYIHSNPSYNHSFSESPVFHGRRIPSKEVRWGNVNMIEAERRLIS 180

Query: 224 NALLDISNERFILLSEACIPLFNFSTVYSFLINSTMKSFIMSYDEPGNVGRGRYRNKMFP 283
           NALLDISNERF+LLSEACIPLFNFST+YSFL+NSTMKSFIMSYDEP NVGRGRYR KMFP
Sbjct: 181 NALLDISNERFVLLSEACIPLFNFSTIYSFLMNSTMKSFIMSYDEPSNVGRGRYRAKMFP 240

Query: 284 PISLKQWRKGSQWFEMDRETAVAVVSDMKYFPVFQNYCKGQCYSDEHYLPTLVNILGWNR 343
           PISLKQWRKGSQWFEMDR+TAVA+VSD KYFPVFQ YCKGQCYSDEHYLPTLVN+LGW+R
Sbjct: 241 PISLKQWRKGSQWFEMDRDTAVAIVSDRKYFPVFQKYCKGQCYSDEHYLPTLVNVLGWDR 300

Query: 344 NANRSLTWVDWSKGGPHPTRFSWSDINVELFQRLRNQTHKCIKTKLEGTGVCFLFARKFA 403
           NANRSLTWVDWSKGGPHPTRFS SDI+VEL Q+LRNQT +C K+K EGTGVCFLFARKF+
Sbjct: 301 NANRSLTWVDWSKGGPHPTRFSRSDIHVELLQKLRNQT-ECTKSKTEGTGVCFLFARKFS 360

Query: 404 PNTLGRLMKIAPKAMHFGR 423
           PNTL RLMKIAPKA+HFGR
Sbjct: 361 PNTLERLMKIAPKALHFGR 374

BLAST of Lsi09G006180 vs. NCBI nr
Match: XP_038906960.1 (glycosyltransferase BC10-like [Benincasa hispida])

HSP 1 Score: 740.0 bits (1909), Expect = 1.2e-209
Identity = 354/379 (93.40%), Postives = 365/379 (96.31%), Query Frame = 0

Query: 44  MKSQIQSPKLIHIHLSFFNVVPYILLFTAGITAGAFLTFYLSNFSISLNLTQIPSSSFFP 103
           MKSQIQSPKLI IHLSFFNVVPY+LLFTAGITAG F TFYLSNFSISLNLTQIPSSSFFP
Sbjct: 1   MKSQIQSPKLIQIHLSFFNVVPYVLLFTAGITAGVFFTFYLSNFSISLNLTQIPSSSFFP 60

Query: 104 VTGGRVGLEEYLKPPEVMHDMEDEELLWRSSMTAGIKKFPFRRVPKIAFMFLTKGPVYLA 163
           VTGGRVGLEEYLKPPEVMHDMEDEELLWR+SM A IKKFPFRRVPKIAFMFLTKGPVYLA
Sbjct: 61  VTGGRVGLEEYLKPPEVMHDMEDEELLWRASMAAEIKKFPFRRVPKIAFMFLTKGPVYLA 120

Query: 164 PLWEEFFKGNEGLYSVYVHSDPSYNHSFPESPVFHGRRIPSKKVGWGKVNMIEAERRLLS 223
           PLWEEFFKGNEGLYSVYVHSDPSYNHSFPESP FHGRR+PSKKVGWGKVNMIEAERRLLS
Sbjct: 121 PLWEEFFKGNEGLYSVYVHSDPSYNHSFPESPAFHGRRVPSKKVGWGKVNMIEAERRLLS 180

Query: 224 NALLDISNERFILLSEACIPLFNFSTVYSFLINSTMKSFIMSYDEPGNVGRGRYRNKMFP 283
           NALLDISNERF+LLSEACIPLFNFSTVYSFLINSTMKSFIMSYDEPGNVGRGRYRNKMFP
Sbjct: 181 NALLDISNERFVLLSEACIPLFNFSTVYSFLINSTMKSFIMSYDEPGNVGRGRYRNKMFP 240

Query: 284 PISLKQWRKGSQWFEMDRETAVAVVSDMKYFPVFQNYCKGQCYSDEHYLPTLVNILGWNR 343
           PISLKQWRKGSQWFEMDR+TA+ VVSD KYFPVFQNYCKGQCYSDEHYLPTLVN+LGW+R
Sbjct: 241 PISLKQWRKGSQWFEMDRDTAITVVSDKKYFPVFQNYCKGQCYSDEHYLPTLVNVLGWDR 300

Query: 344 NANRSLTWVDWSKGGPHPTRFSWSDINVELFQRLRNQTHKCIKTKLEGTGVCFLFARKFA 403
           NANRSLTWVDWSKGGPHP  FS SDI+VELFQRLRNQT +C KTK+EGTGVCFLFARKFA
Sbjct: 301 NANRSLTWVDWSKGGPHPVIFSRSDIHVELFQRLRNQT-ECTKTKMEGTGVCFLFARKFA 360

Query: 404 PNTLGRLMKIAPKAMHFGR 423
           PNTL RLMKIAPKAMHFGR
Sbjct: 361 PNTLERLMKIAPKAMHFGR 378

BLAST of Lsi09G006180 vs. NCBI nr
Match: XP_008467193.1 (PREDICTED: uncharacterized protein LOC103504601 [Cucumis melo] >TYK00270.1 core-2/I-branching enzyme [Cucumis melo var. makuwa])

HSP 1 Score: 734.2 bits (1894), Expect = 6.4e-208
Identity = 349/379 (92.08%), Postives = 366/379 (96.57%), Query Frame = 0

Query: 44  MKSQIQSPKLIHIHLSFFNVVPYILLFTAGITAGAFLTFYLSNFSISLNLTQIPSSSFFP 103
           MKSQ QSPKLIHIHL+FFNVVPYILLFT GI+AG FLTFYLSNFSISLNLTQIPSS FFP
Sbjct: 1   MKSQFQSPKLIHIHLTFFNVVPYILLFTVGISAGVFLTFYLSNFSISLNLTQIPSSDFFP 60

Query: 104 VTGGRVGLEEYLKPPEVMHDMEDEELLWRSSMTAGIKKFPFRRVPKIAFMFLTKGPVYLA 163
           VTGGRVGLEE+LKPPEVMHDM+DEELLWR+SMTAGIKKFPF+RVPKIAFMFLTKGPVYLA
Sbjct: 61  VTGGRVGLEEFLKPPEVMHDMDDEELLWRASMTAGIKKFPFQRVPKIAFMFLTKGPVYLA 120

Query: 164 PLWEEFFKGNEGLYSVYVHSDPSYNHSFPESPVFHGRRIPSKKVGWGKVNMIEAERRLLS 223
           PLWEEFFKGNE LYSVYVHSDPSYNHS PESPVFHGRRIPSKKVGWGKVNMIEAERRL+S
Sbjct: 121 PLWEEFFKGNERLYSVYVHSDPSYNHSSPESPVFHGRRIPSKKVGWGKVNMIEAERRLIS 180

Query: 224 NALLDISNERFILLSEACIPLFNFSTVYSFLINSTMKSFIMSYDEPGNVGRGRYRNKMFP 283
           NALLDISNERF+LLSE+CIPLFNFSTVYSFLINSTMKSFIMSYDEPGNVGRGRYRNKMFP
Sbjct: 181 NALLDISNERFVLLSESCIPLFNFSTVYSFLINSTMKSFIMSYDEPGNVGRGRYRNKMFP 240

Query: 284 PISLKQWRKGSQWFEMDRETAVAVVSDMKYFPVFQNYCKGQCYSDEHYLPTLVNILGWNR 343
           PISLKQWRKGSQWFEMDR+TAVAVVSD KYFPVFQNYCKGQCYSDEHYLPTLVN+LGW+R
Sbjct: 241 PISLKQWRKGSQWFEMDRDTAVAVVSDKKYFPVFQNYCKGQCYSDEHYLPTLVNVLGWDR 300

Query: 344 NANRSLTWVDWSKGGPHPTRFSWSDINVELFQRLRNQTHKCIKTKLEGTGVCFLFARKFA 403
           NANRSLTWVDWSKGGPHP RFS SDI+VEL QRLRNQT +C K+K+EGTGVCFLFARKFA
Sbjct: 301 NANRSLTWVDWSKGGPHPARFSRSDIHVELIQRLRNQTGECRKSKMEGTGVCFLFARKFA 360

Query: 404 PNTLGRLMKIAPKAMHFGR 423
           PNTL RLMKIAPKAM+FGR
Sbjct: 361 PNTLERLMKIAPKAMYFGR 379

BLAST of Lsi09G006180 vs. NCBI nr
Match: XP_023550533.1 (uncharacterized protein LOC111808649 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 718.0 bits (1852), Expect = 4.8e-203
Identity = 341/379 (89.97%), Postives = 362/379 (95.51%), Query Frame = 0

Query: 44  MKSQIQSPKLIHIHLSFFNVVPYILLFTAGITAGAFLTFYLSNFSISLNLTQIPSSSFFP 103
           MKSQIQSPKLIHIHLSFFNVVPYILLFTAGITAG FLTFYLSNFSISLNLTQIP S   P
Sbjct: 1   MKSQIQSPKLIHIHLSFFNVVPYILLFTAGITAGVFLTFYLSNFSISLNLTQIPPSISSP 60

Query: 104 VTGGRVGLEEYLKPPEVMHDMEDEELLWRSSMTAGIKKFPFRRVPKIAFMFLTKGPVYLA 163
           VTG RVGLEEYLKPPEVMHDMEDEELLWR+SM AGI++FPFRRVPK+AFMFL++GP+YLA
Sbjct: 61  VTGPRVGLEEYLKPPEVMHDMEDEELLWRASMAAGIREFPFRRVPKVAFMFLSRGPMYLA 120

Query: 164 PLWEEFFKGNEGLYSVYVHSDPSYNHSFPESPVFHGRRIPSKKVGWGKVNMIEAERRLLS 223
           PLWEEFFKGNEGLYSVY+HSDPSYNHSFPESPVFHGRRIPSK+VGWGKVNMIEAERRL+S
Sbjct: 121 PLWEEFFKGNEGLYSVYIHSDPSYNHSFPESPVFHGRRIPSKEVGWGKVNMIEAERRLIS 180

Query: 224 NALLDISNERFILLSEACIPLFNFSTVYSFLINSTMKSFIMSYDEPGNVGRGRYRNKMFP 283
           NALLDISNERF+LLSEACIPLFN STVYSFL+NSTMKSF+MSYDEP NVGRGRYR KMFP
Sbjct: 181 NALLDISNERFVLLSEACIPLFNLSTVYSFLMNSTMKSFVMSYDEPSNVGRGRYRAKMFP 240

Query: 284 PISLKQWRKGSQWFEMDRETAVAVVSDMKYFPVFQNYCKGQCYSDEHYLPTLVNILGWNR 343
           PISLKQWRKGSQWFEMDR+TAVAVVSD KYFPVFQ YCKGQCYSDEHYLPTLVN+LGW+R
Sbjct: 241 PISLKQWRKGSQWFEMDRDTAVAVVSDRKYFPVFQKYCKGQCYSDEHYLPTLVNVLGWDR 300

Query: 344 NANRSLTWVDWSKGGPHPTRFSWSDINVELFQRLRNQTHKCIKTKLEGTGVCFLFARKFA 403
           NANRSLTWVDWSKGGPHPTRFS SDI+VEL QRLRNQT +C K+K EGTGVCFLFARKF+
Sbjct: 301 NANRSLTWVDWSKGGPHPTRFSRSDIHVELLQRLRNQT-ECTKSKTEGTGVCFLFARKFS 360

Query: 404 PNTLGRLMKIAPKAMHFGR 423
           PNTLGRLMKIAPKA+HFGR
Sbjct: 361 PNTLGRLMKIAPKALHFGR 378

BLAST of Lsi09G006180 vs. NCBI nr
Match: XP_022939468.1 (uncharacterized protein LOC111445363 [Cucurbita moschata])

HSP 1 Score: 716.5 bits (1848), Expect = 1.4e-202
Identity = 340/379 (89.71%), Postives = 362/379 (95.51%), Query Frame = 0

Query: 44  MKSQIQSPKLIHIHLSFFNVVPYILLFTAGITAGAFLTFYLSNFSISLNLTQIPSSSFFP 103
           MKSQIQSPKLIHIHLSFFNVVPYILLFTAGITAG FLTFYLSNFSI+LNLTQIP S   P
Sbjct: 1   MKSQIQSPKLIHIHLSFFNVVPYILLFTAGITAGVFLTFYLSNFSINLNLTQIPPSISSP 60

Query: 104 VTGGRVGLEEYLKPPEVMHDMEDEELLWRSSMTAGIKKFPFRRVPKIAFMFLTKGPVYLA 163
           VTG RVGLEEYLKPPEVMHDMED+ELLWR+SM AGI++FPFRRVPK+AFMFLT+GP+YLA
Sbjct: 61  VTGPRVGLEEYLKPPEVMHDMEDQELLWRASMAAGIREFPFRRVPKVAFMFLTRGPMYLA 120

Query: 164 PLWEEFFKGNEGLYSVYVHSDPSYNHSFPESPVFHGRRIPSKKVGWGKVNMIEAERRLLS 223
           PLWEEFFKGNEGLYSVY+HSDPSYNHSFPESPVFHGRRIPSK+VGWGKVNMIEAERRL+S
Sbjct: 121 PLWEEFFKGNEGLYSVYIHSDPSYNHSFPESPVFHGRRIPSKQVGWGKVNMIEAERRLIS 180

Query: 224 NALLDISNERFILLSEACIPLFNFSTVYSFLINSTMKSFIMSYDEPGNVGRGRYRNKMFP 283
           NALLDISNERF+LLSEACIPLFNFSTVYSFL+NSTMKSFIMSYDEP NVGRGRYR KMFP
Sbjct: 181 NALLDISNERFVLLSEACIPLFNFSTVYSFLMNSTMKSFIMSYDEPSNVGRGRYRAKMFP 240

Query: 284 PISLKQWRKGSQWFEMDRETAVAVVSDMKYFPVFQNYCKGQCYSDEHYLPTLVNILGWNR 343
           PISLKQWRKGSQWFEMDR+TAVAVVSD KYFPVF  YCKGQCYSDEHY+PTLVN+LGW+R
Sbjct: 241 PISLKQWRKGSQWFEMDRDTAVAVVSDRKYFPVFVKYCKGQCYSDEHYMPTLVNVLGWDR 300

Query: 344 NANRSLTWVDWSKGGPHPTRFSWSDINVELFQRLRNQTHKCIKTKLEGTGVCFLFARKFA 403
           NANRSLTWVDWSKGGPHPTRFS SDI+VEL QRLRNQT +C K+K EGTGVCFLFARKF+
Sbjct: 301 NANRSLTWVDWSKGGPHPTRFSRSDIHVELLQRLRNQT-ECTKSKTEGTGVCFLFARKFS 360

Query: 404 PNTLGRLMKIAPKAMHFGR 423
           PNTLGRLMKIAPKA+HFGR
Sbjct: 361 PNTLGRLMKIAPKALHFGR 378

BLAST of Lsi09G006180 vs. NCBI nr
Match: KAG6579216.1 (Glycosyltransferase BC10, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 715.7 bits (1846), Expect = 2.4e-202
Identity = 339/379 (89.45%), Postives = 362/379 (95.51%), Query Frame = 0

Query: 44  MKSQIQSPKLIHIHLSFFNVVPYILLFTAGITAGAFLTFYLSNFSISLNLTQIPSSSFFP 103
           MK+QIQSPKLIHIHLSFFNVVPYILLFTAGITAG FLTFYLSNFSISLNLTQIP S   P
Sbjct: 1   MKTQIQSPKLIHIHLSFFNVVPYILLFTAGITAGVFLTFYLSNFSISLNLTQIPPSISSP 60

Query: 104 VTGGRVGLEEYLKPPEVMHDMEDEELLWRSSMTAGIKKFPFRRVPKIAFMFLTKGPVYLA 163
           +TG RVGLEEYLKPPEVMHDMEDEELLWR+SM AGI++FPFRRVPK+AFMFLT+GP+YLA
Sbjct: 61  LTGPRVGLEEYLKPPEVMHDMEDEELLWRASMAAGIREFPFRRVPKVAFMFLTRGPMYLA 120

Query: 164 PLWEEFFKGNEGLYSVYVHSDPSYNHSFPESPVFHGRRIPSKKVGWGKVNMIEAERRLLS 223
           PLWEEFFKGNEGLYSVY+HSDPSYNHSFPE+PVFHGRRIPSK+VGWGKVNMIEAERRL+S
Sbjct: 121 PLWEEFFKGNEGLYSVYIHSDPSYNHSFPETPVFHGRRIPSKEVGWGKVNMIEAERRLIS 180

Query: 224 NALLDISNERFILLSEACIPLFNFSTVYSFLINSTMKSFIMSYDEPGNVGRGRYRNKMFP 283
           NALLDISNERF+LLSEACIPLFNFSTVYSFL+NSTMKSFIMSYDEP NVGRGRYR KMFP
Sbjct: 181 NALLDISNERFVLLSEACIPLFNFSTVYSFLMNSTMKSFIMSYDEPSNVGRGRYRAKMFP 240

Query: 284 PISLKQWRKGSQWFEMDRETAVAVVSDMKYFPVFQNYCKGQCYSDEHYLPTLVNILGWNR 343
           PISLKQWRKGSQWFEMDR+TAVAVVSD KYFPVF  YCKGQCYSDEHYLPTLVN+LGW+R
Sbjct: 241 PISLKQWRKGSQWFEMDRDTAVAVVSDRKYFPVFVKYCKGQCYSDEHYLPTLVNVLGWDR 300

Query: 344 NANRSLTWVDWSKGGPHPTRFSWSDINVELFQRLRNQTHKCIKTKLEGTGVCFLFARKFA 403
           NANRSLTWVDWSKGGPHPTRFS SD++VEL QRLRNQT +C K+K EGTGVCFLFARKF+
Sbjct: 301 NANRSLTWVDWSKGGPHPTRFSRSDVHVELLQRLRNQT-ECTKSKTEGTGVCFLFARKFS 360

Query: 404 PNTLGRLMKIAPKAMHFGR 423
           PNTLGRLMKIAPKA+HFGR
Sbjct: 361 PNTLGRLMKIAPKALHFGR 378

BLAST of Lsi09G006180 vs. TAIR 10
Match: AT1G68390.1 (Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family protein )

HSP 1 Score: 403.7 bits (1036), Expect = 1.9e-112
Identity = 202/381 (53.02%), Postives = 263/381 (69.03%), Query Frame = 0

Query: 60  FFNVVPYILLFTAGITAGAFLTFYLSNFSISLNLTQIPSSSFF----------------- 119
           F N++ Y L+   GI  G  L   L NFS + +L+    S  F                 
Sbjct: 31  FLNLLSYSLILCCGIIIGILLHSSLQNFSSNSSLSIQRISQLFIVSSLPPSPPPPPPPSP 90

Query: 120 PVTGGRVGLEEYLKPPE-VMHDMEDEELLWRSSMTAGIKKFPFRRVPKIAFMFLTKGPVY 179
           P    + GL+ +++PPE +MHDMEDEELLWR+SM   IK +PF R PK+AFMF+TKG + 
Sbjct: 91  PSEPEQNGLKSFIEPPEKLMHDMEDEELLWRASMAPKIKNYPFPRTPKVAFMFMTKGHLP 150

Query: 180 LAPLWEEFFKGNEGLYSVYVHSDPSYNHSFPESPVFHGRRIPSKKVGWGKVNMIEAERRL 239
           LA LWE FF+G+EGL+++YVHS PSYN S PE  VF GR IPSK+V WG VNM+EAE+RL
Sbjct: 151 LARLWERFFRGHEGLFTIYVHSYPSYNQSDPEDSVFRGRHIPSKRVDWGYVNMVEAEQRL 210

Query: 240 LSNALLDISNERFILLSEACIPLFNFSTVYSFLINSTMKSFIMSYDEPGNVGRGRYRNKM 299
           L+NALLDISNERF+LLSE+CIPLFNF+TVYS+LINST ++ + SYD+ G VGRGRY   M
Sbjct: 211 LANALLDISNERFVLLSESCIPLFNFTTVYSYLINST-QTHVESYDQLGGVGRGRYSPLM 270

Query: 300 FPPISLKQWRKGSQWFEMDRETAVAVVSDMKYFPVFQNYCKGQCYSDEHYLPTLVNILG- 359
            P + L+ WRKGSQW E+DR  A+ ++SD  Y+P+F +YC   CY+DEHY+PTL+NI   
Sbjct: 271 QPHVQLRHWRKGSQWIEVDRAMALEIISDRIYWPLFYSYCHHGCYADEHYIPTLLNIKSS 330

Query: 360 -WNRNANRSLTWVDWSKGGPHPTRFSWSDINVELFQRLRNQTHKCIKTKLEGTGVCFLFA 419
              RN+NR+LTWVDWSKGGPHP RF   ++  E  + LR+   +C+    E T +C+LFA
Sbjct: 331 LKRRNSNRTLTWVDWSKGGPHPNRFIRHEVTAEFMENLRS-GGECLYNG-EETNICYLFA 390

Query: 420 RKFAPNTLGRLMKIAPKAMHF 421
           RKF P  L RL++++   +HF
Sbjct: 391 RKFLPTALDRLLRLSRTVLHF 408

BLAST of Lsi09G006180 vs. TAIR 10
Match: AT5G11730.1 (Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family protein )

HSP 1 Score: 364.0 bits (933), Expect = 1.6e-100
Identity = 181/358 (50.56%), Postives = 244/358 (68.16%), Query Frame = 0

Query: 77  GAFLTFYLSNFSISL---------NLTQIPSSSFFPVTGGRV-GLEEYLKPPEV-MHDME 136
           G FLTF ++ F IS+         ++    +SSF P   G    L ++++PP V MH+M 
Sbjct: 35  GLFLTFSVTVFIISVSTIKYTGIQSVVTTVTSSFVPCREGEPNSLSKWIQPPAVLMHNMS 94

Query: 137 DEELLWRSSMTAGIKKFPFRRVPKIAFMFLTKGPVYLAPLWEEFFKGNEGLYSVYVHSDP 196
           DEELLWR+S     K++PF+RVPK+AFMFLTKGP+ LA LWE F KG++GLYSVY+H  P
Sbjct: 95  DEELLWRASFWPRRKEYPFKRVPKVAFMFLTKGPLPLASLWERFLKGHKGLYSVYLHPHP 154

Query: 197 SYNHSFPESPVFHGRRIPSKKVGWGKVNMIEAERRLLSNALLDISNERFILLSEACIPLF 256
           S+   FP S VFH R+IPS+   WG+++M +AE+RLL+NALLD+SNE F+L+SE+CIPL+
Sbjct: 155 SFTAKFPASSVFHRRQIPSQVAEWGRMSMCDAEKRLLANALLDVSNEWFVLVSESCIPLY 214

Query: 257 NFSTVYSFLINSTMKSFIMSYDEPGNVGRGRYRNKMFPPISLKQWRKGSQWFEMDRETAV 316
           NF+T+YS+L  S   SF+ ++D+PG  GRGRY   M P + L +WRKGSQWFE++R+ A 
Sbjct: 215 NFTTIYSYLSRS-KHSFMGAFDDPGPFGRGRYNGNMEPEVPLTKWRKGSQWFEVNRDLAA 274

Query: 317 AVVSDMKYFPVFQNYCKGQCYSDEHYLPTLVNILGWNRNANRSLTWVDWSKGGPHPTRFS 376
            +V D  Y+P F+ +C+  CY DEHY PT++ I      ANRSLTWVDWS+GGPHP  F 
Sbjct: 275 TIVKDTLYYPKFKEFCRPACYVDEHYFPTMLTIEKPTVLANRSLTWVDWSRGGPHPATFG 334

Query: 377 WSDINVELFQRL---RNQTHKCIKTKLEGTGVCFLFARKFAPNTLGRLMKIAPKAMHF 421
            SDI    F ++   RN ++         T +C+LFARKFAP+ L  L+ IAPK + F
Sbjct: 335 RSDITENFFGKIFDGRNCSYNG-----RNTSMCYLFARKFAPSALEPLLHIAPKILGF 386

BLAST of Lsi09G006180 vs. TAIR 10
Match: AT1G68380.1 (Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family protein )

HSP 1 Score: 356.3 bits (913), Expect = 3.4e-98
Identity = 194/383 (50.65%), Postives = 254/383 (66.32%), Query Frame = 0

Query: 52  KLIHIHLSFFNVVPYILLFTAGITAGAFLTFYLSNFSISLNLT-QIPSSSFF-----PVT 111
           KL+H    F N++ Y  +   G+  G  +   L   S   +LT Q  S  FF     P+ 
Sbjct: 20  KLLH---HFHNLLLYFFILWIGVIVGIIVYPSLQGLSSPSSLTIQSVSQLFFVTPPPPIL 79

Query: 112 GGRV---GLEEYLKP-PEVMHDMEDEELLWRSSMTAGIKKFPFRRVPKIAFMFLTKGPVY 171
                  GL+ +L P   +MHDMED ELLWR+SM   I+ +P+ R+PK+AFMFLT GP+ 
Sbjct: 80  SPSFQDNGLDMFLIPLKNIMHDMEDNELLWRASMDPKIRDYPYPRIPKVAFMFLTWGPLP 139

Query: 172 LAPLWEEFFKGNEGLYSVYVHSDPSYNHSFPESPVFHGRRIPSKKVGWGKVNMIEAERRL 231
           LAPLWE FF+G+EGL+++YVH++ SY+   P+  VF+GRRIPSK+V WG  NM+EAERRL
Sbjct: 140 LAPLWERFFRGHEGLFTIYVHTNSSYDEFMPQDSVFYGRRIPSKRVDWGNANMVEAERRL 199

Query: 232 LSNALLDISNERFILLSEACIPLFNFSTVYSFLINSTMKSFIMSYDEPGNVGRGRYRNKM 291
           L+NALLDI+NERFILLSE+CIPLFNFSTVYSFLI+ST+ + + SYD    +GR RY  +M
Sbjct: 200 LANALLDINNERFILLSESCIPLFNFSTVYSFLIDSTL-THVDSYDL--TIGRVRYDRRM 259

Query: 292 FPPISLKQWRKGSQWFEMDRETAVAVVSDMKYFPVFQNYCKGQCYSDEHYLPTLVNI--- 351
           +P I + QWRKGSQWFE+DR  A+ VVSD  Y+P+F+ Y +     DEHY+PTL+N+   
Sbjct: 260 YPHIRMHQWRKGSQWFELDRAMALEVVSDTFYWPIFKAYSR---CPDEHYIPTLLNMRPS 319

Query: 352 LGWNRNANRSLTWVDWSKGGPHPTRFSWSDINVELFQRLR-NQTHKCIKTKLEGTGVCFL 411
           LG  RNANR+LTW DWSK   HP  F   ++NVE  + LR      C K       +CFL
Sbjct: 320 LGL-RNANRTLTWTDWSKRRAHPRLFGEWEVNVEFLEWLRMKSVGDCKKNGENKMRLCFL 379

Query: 412 FARKFAPNTLGRLMKIAPKAMHF 421
           FARKF+   L  L+++A   M+F
Sbjct: 380 FARKFSSTALDELLRLASIVMYF 392

BLAST of Lsi09G006180 vs. TAIR 10
Match: AT1G10280.1 (Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family protein )

HSP 1 Score: 354.4 bits (908), Expect = 1.3e-97
Identity = 157/310 (50.65%), Postives = 222/310 (71.61%), Query Frame = 0

Query: 111 LEEYLKPPEVMHDMEDEELLWRSSMTAGIKKFPFRRVPKIAFMFLTKGPVYLAPLWEEFF 170
           ++ +++P  + H M D+EL WR+SM    +++P+ RVPK+AFMFLT+GP+ + PLWE+FF
Sbjct: 105 IDGFVRPENLSHGMTDDELFWRASMVPVKEEYPYDRVPKVAFMFLTRGPLPMLPLWEKFF 164

Query: 171 KGNEGLYSVYVHSDPSYNHSFPESPVFHGRRIPSKKVGWGKVNMIEAERRLLSNALLDIS 230
           KGNE   SVYVH+ P Y+ +      F+ R+IPS++V WG   + +AE+RLL+NALLD S
Sbjct: 165 KGNEKYLSVYVHTPPGYDMNVSRDSPFYDRQIPSQRVEWGSPLLTDAEKRLLANALLDFS 224

Query: 231 NERFILLSEACIPLFNFSTVYSFLINSTMKSFIMSYDEPGNVGRGRYRNKMFPPISLKQW 290
           NERF+LLSE+C+P++NFSTVY++LINS   SF+ SYDEP   GRGRY  KM P I L  W
Sbjct: 225 NERFVLLSESCVPVYNFSTVYTYLINSAY-SFVDSYDEPTRYGRGRYSRKMLPDIKLHHW 284

Query: 291 RKGSQWFEMDRETAVAVVSDMKYFPVFQNYCKGQCYSDEHYLPTLVNILGWNRNANRSLT 350
           RKGSQWFE++R+ A+ ++SD KY+ +F+ +C+  CY DEHY+PT +N+   + NANRS+T
Sbjct: 285 RKGSQWFEVNRKIAIYIISDSKYYSLFKQFCRPACYPDEHYIPTFLNMFHGSMNANRSVT 344

Query: 351 WVDWSKGGPHPTRFSWSDINVELFQRLRNQTHKCIKTKLEGTGVCFLFARKFAPNTLGRL 410
           WVDWS GGPHP  ++ ++I     Q +R     C+  + E T +CFLFARKF+P+ L  L
Sbjct: 345 WVDWSIGGPHPATYAAANITEGFLQSIRKNETDCLYNE-EPTSLCFLFARKFSPSALAPL 404

Query: 411 MKIAPKAMHF 421
           M ++   + F
Sbjct: 405 MNLSSTVLGF 412

BLAST of Lsi09G006180 vs. TAIR 10
Match: AT5G25970.1 (Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family protein )

HSP 1 Score: 349.0 bits (894), Expect = 5.4e-96
Identity = 182/364 (50.00%), Postives = 239/364 (65.66%), Query Frame = 0

Query: 65  PYILLFTAGITAGAFLTFYLSNFSISL---------NLTQIPSSSFFPVTGGRVGLEEYL 124
           PY LL    +  G FL F +  F IS+         ++    +SSF P       L++++
Sbjct: 29  PYKLL----LLLGFFLAFTVVLFFISVSTIKYYGINSVVTSVTSSFVPCHEKINDLDKWI 88

Query: 125 KPPEV-MHDMEDEELLWRSSMTAGIKKFPFRRVPKIAFMFLTKGPVYLAPLWEEFFKGNE 184
           KP  V MH+M DEELLW +S     K++PF RVPKIAFMFLT GP+ LAPLWE   KG+E
Sbjct: 89  KPLVVLMHNMSDEELLWGASFMPRRKEYPFNRVPKIAFMFLTMGPLPLAPLWERLLKGHE 148

Query: 185 GLYSVYVHSDPSYNHSFPESPVFHGRRIPSKKVGWGKVNMIEAERRLLSNALLDISNERF 244
            LYSVY+HS  S +  FP S VF+ R IPS+   WG++ M +AERRLL+NALLDISNE F
Sbjct: 149 KLYSVYIHSPVSSSAKFPASSVFYRRHIPSQVAEWGRMTMCDAERRLLANALLDISNEWF 208

Query: 245 ILLSEACIPLFNFSTVYSFLINSTMKSFIMSYDEPGNVGRGRYRNKMFPPISLKQWRKGS 304
           +LLSE+CIPLFNF+T+Y+++  S   SF+ S+D+PG  GRGRY   M P + + QWRKGS
Sbjct: 209 VLLSESCIPLFNFTTIYTYMTKSE-HSFMGSFDDPGAYGRGRYHGNMAPEVFIDQWRKGS 268

Query: 305 QWFEMDRETAVAVVSDMKYFPVFQNYCKGQCYSDEHYLPTLVNILGWNRNANRSLTWVDW 364
           QWFE++RE AV++V D  Y+P F+ +C+  CY DEHY PT++ I      ANRS+TWVDW
Sbjct: 269 QWFEINRELAVSIVKDTLYYPKFKEFCQPACYVDEHYFPTMLTIEKPAALANRSVTWVDW 328

Query: 365 SKGGPHPTRFSWSDINVELFQRLRNQTHKCIKTKLEGTGVCFLFARKFAPNTLGRLMKIA 419
           S+GG HP  F   DIN E F R+    +         T +C+LFARKF+P+ L  L++IA
Sbjct: 329 SRGGAHPATFGAQDINEEFFARILKGDNCTYNGGY--TSMCYLFARKFSPSALEPLVQIA 385

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q65XS51.2e-3633.00Glycosyltransferase BC10 OS=Oryza sativa subsp. japonica OX=39947 GN=BC10 PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A5D3BMR23.1e-20892.08Core-2/I-branching enzyme OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A1S3CU903.1e-20892.08uncharacterized protein LOC103504601 OS=Cucumis melo OX=3656 GN=LOC103504601 PE=... [more]
A0A6J1FLQ96.7e-20389.71uncharacterized protein LOC111445363 OS=Cucurbita moschata OX=3662 GN=LOC1114453... [more]
A0A0A0KT171.8e-20088.65Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G576840 PE=4 SV=1[more]
A0A6J1K0523.0e-19587.07uncharacterized protein LOC111489841 OS=Cucurbita maxima OX=3661 GN=LOC111489841... [more]
Match NameE-valueIdentityDescription
XP_038906960.11.2e-20993.40glycosyltransferase BC10-like [Benincasa hispida][more]
XP_008467193.16.4e-20892.08PREDICTED: uncharacterized protein LOC103504601 [Cucumis melo] >TYK00270.1 core-... [more]
XP_023550533.14.8e-20389.97uncharacterized protein LOC111808649 [Cucurbita pepo subsp. pepo][more]
XP_022939468.11.4e-20289.71uncharacterized protein LOC111445363 [Cucurbita moschata][more]
KAG6579216.12.4e-20289.45Glycosyltransferase BC10, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT1G68390.11.9e-11253.02Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family protein [more]
AT5G11730.11.6e-10050.56Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family protein [more]
AT1G68380.13.4e-9850.65Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family protein [more]
AT1G10280.11.3e-9750.65Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family protein [more]
AT5G25970.15.4e-9650.00Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003406Glycosyl transferase, family 14PFAMPF02485Branchcoord: 150..377
e-value: 2.0E-78
score: 263.3
IPR044174Glycosyltransferase BC10-likePANTHERPTHR31042CORE-2/I-BRANCHING BETA-1,6-N-ACETYLGLUCOSAMINYLTRANSFERASE FAMILY PROTEIN-RELATEDcoord: 49..419
NoneNo IPR availablePANTHERPTHR31042:SF113CORE-2/I-BRANCHING ENZYMEcoord: 49..419

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi09G006180.1Lsi09G006180.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0016757 glycosyltransferase activity