Lsi09G005530 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi09G005530
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	NAD(P)-binding Rossmann-fold superfamily protein
Location	chr09: 5876052 .. 5878568 (+)
RNA-Seq Expression	Lsi09G005530
Synteny	Lsi09G005530

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTTTTTGGATTTTACTAAAAATCCAAAAAACAAATGGGTGATTACAAAAGAAGAAACGTTTCCCATTGACTATACCCAAACATTAATTAGACAGCCATAAAACCATTGTAAAGGAAGAACAAGCTTCCTTATCAACTCTCTTTGTTACCATTTTTTTGTTTTTTTTTTTCCCATTTTTTTCCACCATTAAAGTGCCACTCTCTTATTCAAAGCTTTTCAGTTTCTTCCTCATCAATTCCACAACCAAAATCTTTGAAGATTATTCATCACTCTGTTTATCTGTAAAGCATGATCCAGATCCGACTGAGATATCTCTTCAACAATTCGCTTAATTCGAGCTTATTCTTCAATAGGGGATTGTCTTCCCAAGCTGGAAGGTGAAAATTTTATAGTTCTTATTGTTTCCAATTTCATTTCTCTATTTTTTTTTTCTTGTGTAGATTTGAGCTTAAATTACGAGTTTAGTTTCGAAAACTTTGTGTATAATAAGTTTCTCAAATTTGAAAATGTTTATATGAACTTCTAATTTTAGATCTAATAGGTCTATGCTTTCGATTCTTTTATTTAAGGGGATAGTTGATATATTCCACATTTTTTTTAAAACTAATGAATTTATTAGGCATAAAATTGAATTTTGTGTCAAATTCGACATTAAAGTTTTAATTTTGTGTTCAATAAGTTTGTGGATTTCAAAAAATGTCAAATAGGGTTAGGAACTCATTAGACACACCATTAAAAATTTTTAGACCCCATTTGATAACTATTTAGACTTCAGTCGGTAACCATTTATAGTTTTTGAAATTTTTGCTTGTTTTTCTCACAATTTTTTCCTCATGGTTTTCATATTTTCTATGTAGACAATAGAGTCGTTAGCTAATTTTCAAAAACAAAAACAAGGTTTTGGAAATTACTTTTTAAAATTTTTTAAATTTTGACTTTGATTTTGAAAACAATCCTAAAATGTAGACAACAAAATATAGAAAATAATAGATAGATGTGTGGTTACAAGCTTAATTTTCAAATACAAAAAACCAAATGGACTTTAGTGTTTAGTTTTTGGTTTATGAAAAATAAGCTTACAAACGCTACTTTCATCAATTTTTTAAATTTAATGGTCTATTTTTATGAGTGCTTTAAAGATCTCCAAGTTTTAATAATTAAGATATATATATAGCTTTTTCAATTAGAAAAATGTTAGTTTATAATCCAAATCTTTATGGATGAAATGTGAAAAAATCTAAAAATAAAAAGCACAAAACCAAAAAAAATTTAAATCAAAATTTTCATGAACCAAAAACAAAATACAATGTGTTGTTAAACAAAATCTTGGCCATGAAGTTAAGAAGTCGGAGTTGAAAAGTAGGAGTATTGAACTCCACTTTTGTTCAAGAAATTGGTGGGTCCCACTACTAAAAAAACATAAATTTCATATCTTATCAACTCCTAACACTGTGGGCTCCATGAGTTTACAATTCCCTAGACTTCATAACTCCTTACTCCTTGCTATTTCACTCTTCACCCCAAATATCCTTTAAAGATTTATTAGACACCTTTTAAAGTCGAGAAAAATGCCCTAGTTTTAAGATGATTTTGGGTTTGGATATTCAGGCTTGAAGGGAAGATAGCACTGATTACAGGAGCAGCAAGTGGTATTGGAAAGGCAACTGCAGAGAAATTTATAAGTAACGGAGCCAAAGTTGTAATAGCTGACATCAATGAAAAACTTGGCCAACACACAGCCAAACACTTAGGACCAAATGCAACTTTCATCCCTTGTGATGTCACTAAAGAGTCCAATGTCTCTGATGCTGTAGATTTCACTCTCTCTCTTCACAAAAAACTTGACATTATGTACAACAATGCTGGTATTGCTTGCAACACTCCTCCAACCATCTTGGACCTCGATCTCGCCGTCTTTGATAAAGTCATGAACATCAATGTCCGTGGAGTCTTGGCTGGTATCAAACATGCCTCTCGTGTTATGATCCCTCGACGATCAGGATCCATTCTCTGTACGGCTAGTGTTACAGGACTCATGGGCGGACTAGCTCAACACACGTACTCTGTGTCGAAGGTTGCAGTTATAGGCATTGTCAAGTCGTTGGCATCGGAGTTGTGCAAGTATGGTATTCGAGTCAATTGCATTTCGCCATTTCCGATCCCGACGCCGTTCGTTATCGAGGAAATGAGTCGGCTTTTTCCTTGTATTGAAGAGGAGAAACTTGAGAAGATGATATTTGATTTGAGTGGTTTGGAAGGAACAGTTTGTGAAACTAATGATATAGCTAATGCTGCTCTTTATCTTGCCTCTGATGATGCAAAGTATGTAAGTGGCCACAATTTGGTGGTTGATGGAGCCTTTACTTGTTTCAAGAGTTTAAACTTTCCTTTGCCTGATCAAAATTCAATCCAAGGGGATTCTTGAAAAACCACTTGTTTTGGTCATTTGTTGTATGATTTATACTGGAATAAAAAGGTTTAACTTGTGAAGTTTGAAATTGTTTTCAAATATACGA

mRNA sequence

CTTTTTTGGATTTTACTAAAAATCCAAAAAACAAATGGGTGATTACAAAAGAAGAAACGTTTCCCATTGACTATACCCAAACATTAATTAGACAGCCATAAAACCATTGTAAAGGAAGAACAAGCTTCCTTATCAACTCTCTTTGTTACCATTTTTTTGTTTTTTTTTTTCCCATTTTTTTCCACCATTAAAGTGCCACTCTCTTATTCAAAGCTTTTCAGTTTCTTCCTCATCAATTCCACAACCAAAATCTTTGAAGATTATTCATCACTCTGTTTATCTGTAAAGCATGATCCAGATCCGACTGAGATATCTCTTCAACAATTCGCTTAATTCGAGCTTATTCTTCAATAGGGGATTGTCTTCCCAAGCTGGAAGGCTTGAAGGGAAGATAGCACTGATTACAGGAGCAGCAAGTGGTATTGGAAAGGCAACTGCAGAGAAATTTATAAGTAACGGAGCCAAAGTTGTAATAGCTGACATCAATGAAAAACTTGGCCAACACACAGCCAAACACTTAGGACCAAATGCAACTTTCATCCCTTGTGATGTCACTAAAGAGTCCAATGTCTCTGATGCTGTAGATTTCACTCTCTCTCTTCACAAAAAACTTGACATTATGTACAACAATGCTGGTATTGCTTGCAACACTCCTCCAACCATCTTGGACCTCGATCTCGCCGTCTTTGATAAAGTCATGAACATCAATGTCCGTGGAGTCTTGGCTGGTATCAAACATGCCTCTCGTGTTATGATCCCTCGACGATCAGGATCCATTCTCTGTACGGCTAGTGTTACAGGACTCATGGGCGGACTAGCTCAACACACGTACTCTGTGTCGAAGGTTGCAGTTATAGGCATTGTCAAGTCGTTGGCATCGGAGTTGTGCAAGTATGGTATTCGAGTCAATTGCATTTCGCCATTTCCGATCCCGACGCCGTTCGTTATCGAGGAAATGAGTCGGCTTTTTCCTTGTATTGAAGAGGAGAAACTTGAGAAGATGATATTTGATTTGAGTGGTTTGGAAGGAACAGTTTGTGAAACTAATGATATAGCTAATGCTGCTCTTTATCTTGCCTCTGATGATGCAAAGTATGTAAGTGGCCACAATTTGGTGGTTGATGGAGCCTTTACTTGTTTCAAGAGTTTAAACTTTCCTTTGCCTGATCAAAATTCAATCCAAGGGGATTCTTGAAAAACCACTTGTTTTGGTCATTTGTTGTATGATTTATACTGGAATAAAAAGGTTTAACTTGTGAAGTTTGAAATTGTTTTCAAATATACGA

Coding sequence (CDS)

ATGATCCAGATCCGACTGAGATATCTCTTCAACAATTCGCTTAATTCGAGCTTATTCTTCAATAGGGGATTGTCTTCCCAAGCTGGAAGGCTTGAAGGGAAGATAGCACTGATTACAGGAGCAGCAAGTGGTATTGGAAAGGCAACTGCAGAGAAATTTATAAGTAACGGAGCCAAAGTTGTAATAGCTGACATCAATGAAAAACTTGGCCAACACACAGCCAAACACTTAGGACCAAATGCAACTTTCATCCCTTGTGATGTCACTAAAGAGTCCAATGTCTCTGATGCTGTAGATTTCACTCTCTCTCTTCACAAAAAACTTGACATTATGTACAACAATGCTGGTATTGCTTGCAACACTCCTCCAACCATCTTGGACCTCGATCTCGCCGTCTTTGATAAAGTCATGAACATCAATGTCCGTGGAGTCTTGGCTGGTATCAAACATGCCTCTCGTGTTATGATCCCTCGACGATCAGGATCCATTCTCTGTACGGCTAGTGTTACAGGACTCATGGGCGGACTAGCTCAACACACGTACTCTGTGTCGAAGGTTGCAGTTATAGGCATTGTCAAGTCGTTGGCATCGGAGTTGTGCAAGTATGGTATTCGAGTCAATTGCATTTCGCCATTTCCGATCCCGACGCCGTTCGTTATCGAGGAAATGAGTCGGCTTTTTCCTTGTATTGAAGAGGAGAAACTTGAGAAGATGATATTTGATTTGAGTGGTTTGGAAGGAACAGTTTGTGAAACTAATGATATAGCTAATGCTGCTCTTTATCTTGCCTCTGATGATGCAAAGTATGTAAGTGGCCACAATTTGGTGGTTGATGGAGCCTTTACTTGTTTCAAGAGTTTAAACTTTCCTTTGCCTGATCAAAATTCAATCCAAGGGGATTCTTGA

Protein sequence

MIQIRLRYLFNNSLNSSLFFNRGLSSQAGRLEGKIALITGAASGIGKATAEKFISNGAKVVIADINEKLGQHTAKHLGPNATFIPCDVTKESNVSDAVDFTLSLHKKLDIMYNNAGIACNTPPTILDLDLAVFDKVMNINVRGVLAGIKHASRVMIPRRSGSILCTASVTGLMGGLAQHTYSVSKVAVIGIVKSLASELCKYGIRVNCISPFPIPTPFVIEEMSRLFPCIEEEKLEKMIFDLSGLEGTVCETNDIANAALYLASDDAKYVSGHNLVVDGAFTCFKSLNFPLPDQNSIQGDS

Homology

[Close]

BLAST of Lsi09G005530 vs. ExPASy Swiss-Prot
Match: Q94KL7 (Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 PE=1 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 2.6e-65
Identity = 138/274 (50.36%), Postives = 189/274 (68.98%), Query Frame = 0


Query: 24  LSSQAGRLEGKIALITGAASGIGKATAEKFISNGAKVVIADINEKLGQHTAKHLG-PNAT 83
           L++ A RLEGK+ALITG ASGIG+ TA+ F  +GAKV IAD+ ++LG    + +G  N+T
Sbjct: 8   LTAIARRLEGKVALITGGASGIGETTAKLFSQHGAKVAIADVQDELGHSVVEAIGTSNST 67

Query: 84  FIPCDVTKESNVSDAVDFTLSLHKKLDIMYNNAGIACNTPPTILDLDLAVFDKVMNINVR 143
           +I CDVT E  V +AVD T+S + KLDIM++NAGI+    P I+D + A F++V ++NV 
Sbjct: 68  YIHCDVTNEDGVKNAVDNTVSTYGKLDIMFSNAGISDPNRPRIIDNEKADFERVFSVNVT 127

Query: 144 GVLAGIKHASRVMIPRRSGSILCTASVTGLMGGLAQHTYSVSKVAVIGIVKSLASELCKY 203
           GV   +KHA+RVMIP RSG+I+ TAS++  MGG + H Y  SK AV+G+ ++LA EL ++
Sbjct: 128 GVFLCMKHAARVMIPARSGNIISTASLSSTMGGGSSHAYCGSKHAVLGLTRNLAVELGQF 187

Query: 204 GIRVNCISPFPIPTPFVIEEMSRLFPCIE-EEKLEKMIFDLSGLEGTVCETNDIANAALY 263
           GIRVNC+SPF +PT      + + F  I+ EE+ E +I     L+G      D+ANAALY
Sbjct: 188 GIRVNCLSPFGLPT-----ALGKKFSGIKNEEEFENVINFAGNLKGPKFNVEDVANAALY 247

Query: 264 LASDDAKYVSGHNLVVDGAFTCFKSL--NFPLPD 294
           LASD+AKYVSGHNL +DG F+   S+   F  PD
Sbjct: 248 LASDEAKYVSGHNLFIDGGFSVCNSVIKVFQYPD 276

[Close]

BLAST of Lsi09G005530 vs. ExPASy Swiss-Prot
Match: Q9SCU0 (Short-chain dehydrogenase reductase 2a OS=Arabidopsis thaliana OX=3702 GN=SDR2a PE=3 SV=1)

HSP 1 Score: 230.3 bits (586), Expect = 2.8e-59
Identity = 128/265 (48.30%), Postives = 171/265 (64.53%), Query Frame = 0


Query: 30  RLEGKIALITGAASGIGKATAEKFISNGAKVVIADINEKLGQHTAKHL-----GPNATFI 89
           RLEGK+A+ITG A GIGKAT   F  +GA VVIAD++   G   AK L      P   FI
Sbjct: 31  RLEGKVAIITGGAHGIGKATVMLFARHGATVVIADVDNVAGSSLAKSLSSHKTSPMVAFI 90

Query: 90  PCDVTKESNVSDAVDFTLSLHKKLDIMYNNAGIACNTP--PTILDLDLAVFDKVMNINVR 149
            CDV+ E++V + V+ T++ + +LDI++NNAG+  +     +ILD D   FD VM +NVR
Sbjct: 91  SCDVSVEADVENLVNVTVARYGRLDILFNNAGVLGDQKKHKSILDFDADEFDHVMRVNVR 150

Query: 150 GVLAGIKHASRVMIPRR-SGSILCTASVTGLMGGLAQHTYSVSKVAVIGIVKSLASELCK 209
           GV  G+KH +R MI R   G I+ TASV G+MGG+  H Y+ SK A++G+ K+ A EL K
Sbjct: 151 GVGLGMKHGARAMIKRGFKGCIISTASVAGVMGGMGPHAYTASKHAIVGLTKNAACELGK 210

Query: 210 YGIRVNCISPFPIPTPFVIEEMSRLFPCIEE----EKLEKMIFDLSGLEGTVCETNDIAN 269
           YGIRVNCISPF + T  ++    +      E    E++E+ +  L+ L+G     NDIA 
Sbjct: 211 YGIRVNCISPFGVATSMLVNAWRKTSGGDVEDDDVEEMEEFVRSLANLKGETLRANDIAE 270

Query: 270 AALYLASDDAKYVSGHNLVVDGAFT 283
           AALYLASD++KYV+GHNLVVDG  T
Sbjct: 271 AALYLASDESKYVNGHNLVVDGGVT 295

[Close]

BLAST of Lsi09G005530 vs. ExPASy Swiss-Prot
Match: P50160 (Sex determination protein tasselseed-2 OS=Zea mays OX=4577 GN=TS2 PE=2 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 1.1e-58
Identity = 122/282 (43.26%), Postives = 175/282 (62.06%), Query Frame = 0


Query: 30  RLEGKIALITGAASGIGKATAEKFISNGAKVVIADINEKLGQHTAKHLGPNATFIPCDVT 89
           RL+GK+A++TG A GIG+A    F  +GA+VVIADI++  G+  A  LGP  +F+ CDV+
Sbjct: 52  RLDGKVAIVTGGARGIGEAIVRLFAKHGARVVIADIDDAAGEALASALGPQVSFVRCDVS 111

Query: 90  KESNVSDAVDFTLSLH-KKLDIMYNNAGI---ACNTPPTILDLDLAVFDKVMNINVRGVL 149
            E +V  AVD+ LS H  +LD+  NNAG+         +IL  D A FD+V+ +N  G  
Sbjct: 112 VEDDVRRAVDWALSRHGGRLDVYCNNAGVLGRQTRAARSILSFDAAEFDRVLRVNALGAA 171

Query: 150 AGIKHASRVMIPRRSGSILCTASVTGLMGGLAQHTYSVSKVAVIGIVKSLASELCKYGIR 209
            G+KHA+R M PRR+GSI+  ASV  ++GGL  H Y+ SK A++G+ K+ A EL  +G+R
Sbjct: 172 LGMKHAARAMAPRRAGSIVSVASVAAVLGGLGPHAYTASKHAIVGLTKNAACELRAHGVR 231

Query: 210 VNCISPFPIPTPFVIE-------------------EMSRLFPCIEE-EKLEKMIFDLSGL 269
           VNC+SPF + TP +I                    ++    P  +E EK+E+++  L+ L
Sbjct: 232 VNCVSPFGVATPMLINAWRQGHDDATADADRDLDLDLDVTVPSDQEVEKMEEVVRGLATL 291

Query: 270 EGTVCETNDIANAALYLASDDAKYVSGHNLVVDGAFTCFKSL 288
           +G      DIA A L+LASD+A+Y+SGHNLVVDG  T  ++L
Sbjct: 292 KGPTLRPRDIAEAVLFLASDEARYISGHNLVVDGGVTTSRNL 333

[Close]

BLAST of Lsi09G005530 vs. ExPASy Swiss-Prot
Match: F1SWA0 (Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 7.7e-57
Identity = 120/262 (45.80%), Postives = 172/262 (65.65%), Query Frame = 0


Query: 30  RLEGKIALITGAASGIGKATAEKFISNGAKVVIADINEKLGQHTAKHLG--PNATFIPCD 89
           RLEGK+AL+TG ASGIG++ A  FI +GAK+ I D+ ++LGQ  ++ LG  P+A +  CD
Sbjct: 2   RLEGKVALVTGGASGIGESIARLFIEHGAKICIVDVQDELGQQVSQRLGGDPHACYFHCD 61

Query: 90  VTKESNVSDAVDFTLSLHKKLDIMYNNAGIACNTPPTILDLDLAVFDKVMNINVRGVLAG 149
           VT E +V  AVDFT   +  +DIM NNAGI  +    I D D   F KV +INV GV  G
Sbjct: 62  VTVEDDVRRAVDFTAEKYGTIDIMVNNAGITGDKVIDIRDADFNEFKKVFDINVNGVFLG 121

Query: 150 IKHASRVMIPRRSGSILCTASVTGLMGGLAQHTYSVSKVAVIGIVKSLASELCKYGIRVN 209
           +KHA+R+MIP+  GSI+  ASV+ ++ G   H Y+ +K AV+G+ KS+A+EL ++GIRVN
Sbjct: 122 MKHAARIMIPKMKGSIVSLASVSSVIAGAGPHGYTGAKHAVVGLTKSVAAELGRHGIRVN 181

Query: 210 CISPFPIPT----PFVIEEMSRLFPCIEEEKLE---KMIFDLSGLEGTVCETNDIANAAL 269
           C+SP+ +PT    P++ E        ++E+ L      +   + L+G     ND+A A L
Sbjct: 182 CVSPYAVPTRLSMPYLPESE------MQEDALRGFLTFVRSNANLKGVDLMPNDVAEAVL 241

Query: 270 YLASDDAKYVSGHNLVVDGAFT 283
           YLA++++KYVSG NLV+DG F+
Sbjct: 242 YLATEESKYVSGLNLVIDGGFS 257

[Close]

BLAST of Lsi09G005530 vs. ExPASy Swiss-Prot
Match: H9BFQ0 (Tropinone reductase-like 1 OS=Erythroxylum coca OX=289672 PE=2 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 1.3e-56
Identity = 123/256 (48.05%), Postives = 169/256 (66.02%), Query Frame = 0


Query: 30  RLEGKIALITGAASGIGKATAEKFISNGAKVVIADINEKLGQHTAKHLGPNATFIPCDVT 89
           RLEGK+A+ITG ASGIG  TAE F  NGAKVVIADI + LGQ  A  LG  A +I CDV+
Sbjct: 10  RLEGKVAIITGGASGIGACTAELFHENGAKVVIADIQDDLGQALATKLGGKACYIHCDVS 69

Query: 90  KESNVSDAVDFTLSLHKKLDIMYNNAGI--ACNTPPTILDLDLAVFDKVMNINVRGVLAG 149
           KE +V + VD T++ + +LDIM+NNAGI      P ++++ + +  D+++++N+ G   G
Sbjct: 70  KEDDVINLVDTTVAKYGRLDIMFNNAGIIEGQGLPVSVVESEKSDLDRLLSVNLGGAFLG 129

Query: 150 IKHASRVMIPRRSGSILCTASVTGLMGGLAQHTYSVSKVAVIGIVKSLASELCKYGIRVN 209
            KHA+RVM+ +R G IL T+S+   + GL+ H Y+ SK  V G+ K+L  EL KYGIRVN
Sbjct: 130 AKHATRVMVQQRKGCILFTSSLCTSIAGLSGHAYAASKSGVCGLAKNLTPELGKYGIRVN 189

Query: 210 CISPFPIPTPFV-IEEMSRLFPCIEEEKLEKMIFDLSGLEGTVCETNDIANAALYLASDD 269
           CISP+ + T    I E +R       E +E M+ +L  L G     + IA AAL+LASD+
Sbjct: 190 CISPYGLVTGISNISEANR-------ELVEAMLSELGTLSGQTLRADGIAKAALFLASDE 249

Query: 270 AKYVSGHNLVVDGAFT 283
           A YVSG N+VVDG ++
Sbjct: 250 AYYVSGINMVVDGGYS 258

[Close]

BLAST of Lsi09G005530 vs. ExPASy TrEMBL
Match: A0A1S3CT49 (secoisolariciresinol dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103504658 PE=4 SV=1)

HSP 1 Score: 535.0 bits (1377), Expect = 2.0e-148
Identity = 275/302 (91.06%), Postives = 285/302 (94.37%), Query Frame = 0


Query: 1   MIQI-RLRYLFNNSLNSSLFFNRGLSSQAGRLEGKIALITGAASGIGKATAEKFISNGAK 60
           MIQI RLR LFNNSL  S FFNR  SSQ GRLEGKIALITGAASGIGKATA KFISNGAK
Sbjct: 1   MIQIQRLRSLFNNSLTWSFFFNRRFSSQPGRLEGKIALITGAASGIGKATAGKFISNGAK 60

Query: 61  VVIADINEKLGQHTAKHLGPNATFIPCDVTKESNVSDAVDFTLSLHKKLDIMYNNAGIAC 120
           VVIADI EKLGQ TAK LGPNATFI CDVTKESN+SDAVDFT+SLHKKLDIMYNNAGIAC
Sbjct: 61  VVIADIKEKLGQDTAKQLGPNATFIHCDVTKESNISDAVDFTISLHKKLDIMYNNAGIAC 120

Query: 121 NTPPTILDLDLAVFDKVMNINVRGVLAGIKHASRVMIPRRSGSILCTASVTGLMGGLAQH 180
           NTPP+I DLDLAVFDKVMN+NVRGVLAGIKHASRVMIPRRSGSILCTASVTGLMGGLAQH
Sbjct: 121 NTPPSISDLDLAVFDKVMNVNVRGVLAGIKHASRVMIPRRSGSILCTASVTGLMGGLAQH 180

Query: 181 TYSVSKVAVIGIVKSLASELCKYGIRVNCISPFPIPTPFVIEEMSRLFPCIEEEKLEKMI 240
           TYSVSKVAVIGIVKSLASELC+YGIRVNCISPFPIPTPFVIEEM++LFP +EEEKLEKMI
Sbjct: 181 TYSVSKVAVIGIVKSLASELCRYGIRVNCISPFPIPTPFVIEEMTQLFPSVEEEKLEKMI 240

Query: 241 FDLSGLEGTVCETNDIANAALYLASDDAKYVSGHNLVVDGAFTCFKSLNFPLPDQNSIQG 300
           FDLS LEGTVCETNDIANAALYLASDDAKYVSGHNLVVDGAFTCFKSLNF LPDQN+IQG
Sbjct: 241 FDLSALEGTVCETNDIANAALYLASDDAKYVSGHNLVVDGAFTCFKSLNFSLPDQNTIQG 300

Query: 301 DS 302
           +S
Sbjct: 301 ES 302

[Close]

BLAST of Lsi09G005530 vs. ExPASy TrEMBL
Match: A0A0A0KL23 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G171720 PE=4 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 3.2e-146
Identity = 270/302 (89.40%), Postives = 283/302 (93.71%), Query Frame = 0


Query: 1   MIQI-RLRYLFNNSLNSSLFFNRGLSSQAGRLEGKIALITGAASGIGKATAEKFISNGAK 60
           MIQI RLR LFNNSL  S FFNR  SSQ  RL+GKIALITGAASGIGKATAEKFISNGAK
Sbjct: 1   MIQIRRLRSLFNNSLTWSFFFNRRFSSQPARLDGKIALITGAASGIGKATAEKFISNGAK 60

Query: 61  VVIADINEKLGQHTAKHLGPNATFIPCDVTKESNVSDAVDFTLSLHKKLDIMYNNAGIAC 120
           VVIADI EKLGQ TAK LGPNATFI CDVTKESN+SDAVDFT+SLHKKLDIMYNNAGIAC
Sbjct: 61  VVIADIKEKLGQDTAKQLGPNATFIHCDVTKESNISDAVDFTISLHKKLDIMYNNAGIAC 120

Query: 121 NTPPTILDLDLAVFDKVMNINVRGVLAGIKHASRVMIPRRSGSILCTASVTGLMGGLAQH 180
           NTPP+I DLDLAVFDKVMN+NVRGVLAG+KHASRVMIP+RSGSILCTASVTGLMGGLAQH
Sbjct: 121 NTPPSISDLDLAVFDKVMNVNVRGVLAGVKHASRVMIPQRSGSILCTASVTGLMGGLAQH 180

Query: 181 TYSVSKVAVIGIVKSLASELCKYGIRVNCISPFPIPTPFVIEEMSRLFPCIEEEKLEKMI 240
           TYSVSKVAVIGIVKSLASELC+YGIRVNCISPFPIPTPFVIEEM +LFP +EE KLEKMI
Sbjct: 181 TYSVSKVAVIGIVKSLASELCRYGIRVNCISPFPIPTPFVIEEMVQLFPRVEEAKLEKMI 240

Query: 241 FDLSGLEGTVCETNDIANAALYLASDDAKYVSGHNLVVDGAFTCFKSLNFPLPDQNSIQG 300
           FDLS LEGTVCETNDIANAAL+LASDDAKYVSGHNLVVDGAFTCFKSLNFPLPDQN+I G
Sbjct: 241 FDLSALEGTVCETNDIANAALFLASDDAKYVSGHNLVVDGAFTCFKSLNFPLPDQNTIHG 300

Query: 301 DS 302
           +S
Sbjct: 301 ES 302

[Close]

BLAST of Lsi09G005530 vs. ExPASy TrEMBL
Match: A0A5D3DB97 (Secoisolariciresinol dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold480G001200 PE=4 SV=1)

HSP 1 Score: 501.5 bits (1290), Expect = 2.4e-138
Identity = 252/271 (92.99%), Postives = 262/271 (96.68%), Query Frame = 0


Query: 31  LEGKIALITGAASGIGKATAEKFISNGAKVVIADINEKLGQHTAKHLGPNATFIPCDVTK 90
           LEGKIALITGAASGIGKATA KFISNGAKVVIADI EKLGQ TAK LGPNATFI CDVTK
Sbjct: 5   LEGKIALITGAASGIGKATAGKFISNGAKVVIADIKEKLGQDTAKQLGPNATFIHCDVTK 64

Query: 91  ESNVSDAVDFTLSLHKKLDIMYNNAGIACNTPPTILDLDLAVFDKVMNINVRGVLAGIKH 150
           ESN+SDAVDFT+SLHKKLDIMYNNAGIACNTPP+I DLDLAVFDKVMN+NVRGVLAGIKH
Sbjct: 65  ESNISDAVDFTISLHKKLDIMYNNAGIACNTPPSISDLDLAVFDKVMNVNVRGVLAGIKH 124

Query: 151 ASRVMIPRRSGSILCTASVTGLMGGLAQHTYSVSKVAVIGIVKSLASELCKYGIRVNCIS 210
           ASRVMIPRRSGSILCTASVTGLMGGLAQHTYSVSKVAVIGIVKSLASELC+YGIRVNCIS
Sbjct: 125 ASRVMIPRRSGSILCTASVTGLMGGLAQHTYSVSKVAVIGIVKSLASELCRYGIRVNCIS 184

Query: 211 PFPIPTPFVIEEMSRLFPCIEEEKLEKMIFDLSGLEGTVCETNDIANAALYLASDDAKYV 270
           PFPIPTPFVIEEM++LFP +EEEKLEKMIFDLS LEGTVCETNDIANAALYLASDDAKYV
Sbjct: 185 PFPIPTPFVIEEMTQLFPSVEEEKLEKMIFDLSALEGTVCETNDIANAALYLASDDAKYV 244

Query: 271 SGHNLVVDGAFTCFKSLNFPLPDQNSIQGDS 302
           SGHNLVVDGAFTCFKSLNF LPDQN+IQG+S
Sbjct: 245 SGHNLVVDGAFTCFKSLNFSLPDQNTIQGES 275

[Close]

BLAST of Lsi09G005530 vs. ExPASy TrEMBL
Match: A0A5A7UNV0 (Secoisolariciresinol dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G00700 PE=4 SV=1)

HSP 1 Score: 495.7 bits (1275), Expect = 1.3e-136
Identity = 249/268 (92.91%), Postives = 259/268 (96.64%), Query Frame = 0


Query: 34  KIALITGAASGIGKATAEKFISNGAKVVIADINEKLGQHTAKHLGPNATFIPCDVTKESN 93
           KIALITGAASGIGKATA KFISNGAKVVIADI EKLGQ TAK LGPNATFI CDVTKESN
Sbjct: 5   KIALITGAASGIGKATAGKFISNGAKVVIADIKEKLGQDTAKQLGPNATFIHCDVTKESN 64

Query: 94  VSDAVDFTLSLHKKLDIMYNNAGIACNTPPTILDLDLAVFDKVMNINVRGVLAGIKHASR 153
           +SDAVDFT+SLHKKLDIMYNNAGIACNTPP+I DLDLAVFDKVMN+NVRGVLAGIKHASR
Sbjct: 65  ISDAVDFTISLHKKLDIMYNNAGIACNTPPSISDLDLAVFDKVMNVNVRGVLAGIKHASR 124

Query: 154 VMIPRRSGSILCTASVTGLMGGLAQHTYSVSKVAVIGIVKSLASELCKYGIRVNCISPFP 213
           VMIPRRSGSILCTASVTGLMGGLAQHTYSVSKVAVIGIVKSLASELC+YGIRVNCISPFP
Sbjct: 125 VMIPRRSGSILCTASVTGLMGGLAQHTYSVSKVAVIGIVKSLASELCRYGIRVNCISPFP 184

Query: 214 IPTPFVIEEMSRLFPCIEEEKLEKMIFDLSGLEGTVCETNDIANAALYLASDDAKYVSGH 273
           IPTPFVIEEM++LFP +EEEKLEKMIFDLS LEGTVCETNDIANAALYLASDDAKYVSGH
Sbjct: 185 IPTPFVIEEMTQLFPSVEEEKLEKMIFDLSALEGTVCETNDIANAALYLASDDAKYVSGH 244

Query: 274 NLVVDGAFTCFKSLNFPLPDQNSIQGDS 302
           NLVVDGAFTCFKSLNF LPDQN+IQG+S
Sbjct: 245 NLVVDGAFTCFKSLNFSLPDQNTIQGES 272

[Close]

BLAST of Lsi09G005530 vs. ExPASy TrEMBL
Match: A0A6J1K592 (secoisolariciresinol dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111491301 PE=4 SV=1)

HSP 1 Score: 493.8 bits (1270), Expect = 5.0e-136
Identity = 252/288 (87.50%), Postives = 264/288 (91.67%), Query Frame = 0


Query: 8   YLFNNSLNSSLFFNRGLSSQAGRLEGKIALITGAASGIGKATAEKFISNGAKVVIADINE 67
           +L  NSL  S FFNRGLSS AGRLEGKIALITGAASGIGKATA KFISNGAKVV+AD+ E
Sbjct: 5   HLLRNSLTLS-FFNRGLSSHAGRLEGKIALITGAASGIGKATASKFISNGAKVVLADVEE 64

Query: 68  KLGQHTAKHLGPNATFIPCDVTKESNVSDAVDFTLSLHKKLDIMYNNAGIACNTPPTILD 127
           +LGQ TAK LGPNATFIPCDV+ ESNVSDAVDF LSLHK+LDIM NNAGIACNTPP+ILD
Sbjct: 65  ELGQATAKQLGPNATFIPCDVSNESNVSDAVDFALSLHKRLDIMCNNAGIACNTPPSILD 124

Query: 128 LDLAVFDKVMNINVRGVLAGIKHASRVMIPRRSGSILCTASVTGLMGGLAQHTYSVSKVA 187
           LDLAVFDKVMNINVRGVLAGIKHASRVMIP++SGSILCTASVTG+MGGLAQHTYSVSKVA
Sbjct: 125 LDLAVFDKVMNINVRGVLAGIKHASRVMIPQQSGSILCTASVTGVMGGLAQHTYSVSKVA 184

Query: 188 VIGIVKSLASELCKYGIRVNCISPFPIPTPFVIEEMSRLFPCIEEEKLEKMIFDLSGLEG 247
           VIGIVKS+ASELCKYGIRVNCISPF IPTPF I EM  LFP  E+EKLEKMIFDLS LEG
Sbjct: 185 VIGIVKSMASELCKYGIRVNCISPFAIPTPFAINEMCHLFPGTEKEKLEKMIFDLSALEG 244

Query: 248 TVCETNDIANAALYLASDDAKYVSGHNLVVDGAFTCFKSLNFPLPDQN 296
            VCETNDIANAALYLASDDAKYVSGHNLVVDG FTCFK LNFPLPDQN
Sbjct: 245 AVCETNDIANAALYLASDDAKYVSGHNLVVDGGFTCFKRLNFPLPDQN 291

[Close]

BLAST of Lsi09G005530 vs. NCBI nr
Match: XP_038876474.1 (secoisolariciresinol dehydrogenase [Benincasa hispida])

HSP 1 Score: 540.0 bits (1390), Expect = 1.3e-149
Identity = 278/302 (92.05%), Postives = 284/302 (94.04%), Query Frame = 0


Query: 1   MIQIRLRYLFNNSLNSSLFFNRGLSSQAGRLEGKIALITGAASGIGKATAEKFISNGAKV 60
           MIQIRLRYLFN     S FFNRG SS  GRLEGKIALITGAASGIGKATA KFISNGAKV
Sbjct: 1   MIQIRLRYLFN-----SFFFNRGFSSHVGRLEGKIALITGAASGIGKATAGKFISNGAKV 60

Query: 61  VIADINEKLGQHTAKHLGPNATFIPCDVTKESNVSDAVDFTLSLHKKLDIMYNNAGIACN 120
           VIADINEKLGQ T K LGPNATFIPCDVTKESNVSDAVDFTLSLHKKLDIMYNNAGIACN
Sbjct: 61  VIADINEKLGQETVKQLGPNATFIPCDVTKESNVSDAVDFTLSLHKKLDIMYNNAGIACN 120

Query: 121 TPPTILDLDLAVFDKVMNINVRGVLAGIKHASRVMIPRRSGSILCTASVTGLMGGLAQHT 180
           TPP+ILDLDLAVFDKVMNINVRGVLAGIKHASRVMIP+RSGSILCTAS+TGLMGGLAQHT
Sbjct: 121 TPPSILDLDLAVFDKVMNINVRGVLAGIKHASRVMIPQRSGSILCTASITGLMGGLAQHT 180

Query: 181 YSVSKVAVIGIVKSLASELCKYGIRVNCISPFPIPTPFVIEEMSRLFPCIEEEKLEKMIF 240
           YSVSKVAVIGIVKSLASELCKYGIRVNCISPFPIPTPFVIEEM+RLFPC+E+EKLEKMIF
Sbjct: 181 YSVSKVAVIGIVKSLASELCKYGIRVNCISPFPIPTPFVIEEMTRLFPCVEKEKLEKMIF 240

Query: 241 DLSGLEGTVCETNDIANAALYLASDDAKYVSGHNLVVDGAFTCFKSLNFPLPDQNSI-QG 300
           DLS LEGTVCETNDIANAALYLASDDAKYVSGHNLVVDGAFTCFKSLNFPLPDQ  I Q 
Sbjct: 241 DLSALEGTVCETNDIANAALYLASDDAKYVSGHNLVVDGAFTCFKSLNFPLPDQKPILQE 297

Query: 301 DS 302
           DS
Sbjct: 301 DS 297

[Close]

BLAST of Lsi09G005530 vs. NCBI nr
Match: XP_008467261.1 (PREDICTED: secoisolariciresinol dehydrogenase [Cucumis melo])

HSP 1 Score: 535.0 bits (1377), Expect = 4.1e-148
Identity = 275/302 (91.06%), Postives = 285/302 (94.37%), Query Frame = 0


Query: 1   MIQI-RLRYLFNNSLNSSLFFNRGLSSQAGRLEGKIALITGAASGIGKATAEKFISNGAK 60
           MIQI RLR LFNNSL  S FFNR  SSQ GRLEGKIALITGAASGIGKATA KFISNGAK
Sbjct: 1   MIQIQRLRSLFNNSLTWSFFFNRRFSSQPGRLEGKIALITGAASGIGKATAGKFISNGAK 60

Query: 61  VVIADINEKLGQHTAKHLGPNATFIPCDVTKESNVSDAVDFTLSLHKKLDIMYNNAGIAC 120
           VVIADI EKLGQ TAK LGPNATFI CDVTKESN+SDAVDFT+SLHKKLDIMYNNAGIAC
Sbjct: 61  VVIADIKEKLGQDTAKQLGPNATFIHCDVTKESNISDAVDFTISLHKKLDIMYNNAGIAC 120

Query: 121 NTPPTILDLDLAVFDKVMNINVRGVLAGIKHASRVMIPRRSGSILCTASVTGLMGGLAQH 180
           NTPP+I DLDLAVFDKVMN+NVRGVLAGIKHASRVMIPRRSGSILCTASVTGLMGGLAQH
Sbjct: 121 NTPPSISDLDLAVFDKVMNVNVRGVLAGIKHASRVMIPRRSGSILCTASVTGLMGGLAQH 180

Query: 181 TYSVSKVAVIGIVKSLASELCKYGIRVNCISPFPIPTPFVIEEMSRLFPCIEEEKLEKMI 240
           TYSVSKVAVIGIVKSLASELC+YGIRVNCISPFPIPTPFVIEEM++LFP +EEEKLEKMI
Sbjct: 181 TYSVSKVAVIGIVKSLASELCRYGIRVNCISPFPIPTPFVIEEMTQLFPSVEEEKLEKMI 240

Query: 241 FDLSGLEGTVCETNDIANAALYLASDDAKYVSGHNLVVDGAFTCFKSLNFPLPDQNSIQG 300
           FDLS LEGTVCETNDIANAALYLASDDAKYVSGHNLVVDGAFTCFKSLNF LPDQN+IQG
Sbjct: 241 FDLSALEGTVCETNDIANAALYLASDDAKYVSGHNLVVDGAFTCFKSLNFSLPDQNTIQG 300

Query: 301 DS 302
           +S
Sbjct: 301 ES 302

[Close]

BLAST of Lsi09G005530 vs. NCBI nr
Match: XP_004143649.1 (secoisolariciresinol dehydrogenase [Cucumis sativus] >KGN50390.1 hypothetical protein Csa_000609 [Cucumis sativus])

HSP 1 Score: 527.7 bits (1358), Expect = 6.5e-146
Identity = 270/302 (89.40%), Postives = 283/302 (93.71%), Query Frame = 0


Query: 1   MIQI-RLRYLFNNSLNSSLFFNRGLSSQAGRLEGKIALITGAASGIGKATAEKFISNGAK 60
           MIQI RLR LFNNSL  S FFNR  SSQ  RL+GKIALITGAASGIGKATAEKFISNGAK
Sbjct: 1   MIQIRRLRSLFNNSLTWSFFFNRRFSSQPARLDGKIALITGAASGIGKATAEKFISNGAK 60

Query: 61  VVIADINEKLGQHTAKHLGPNATFIPCDVTKESNVSDAVDFTLSLHKKLDIMYNNAGIAC 120
           VVIADI EKLGQ TAK LGPNATFI CDVTKESN+SDAVDFT+SLHKKLDIMYNNAGIAC
Sbjct: 61  VVIADIKEKLGQDTAKQLGPNATFIHCDVTKESNISDAVDFTISLHKKLDIMYNNAGIAC 120

Query: 121 NTPPTILDLDLAVFDKVMNINVRGVLAGIKHASRVMIPRRSGSILCTASVTGLMGGLAQH 180
           NTPP+I DLDLAVFDKVMN+NVRGVLAG+KHASRVMIP+RSGSILCTASVTGLMGGLAQH
Sbjct: 121 NTPPSISDLDLAVFDKVMNVNVRGVLAGVKHASRVMIPQRSGSILCTASVTGLMGGLAQH 180

Query: 181 TYSVSKVAVIGIVKSLASELCKYGIRVNCISPFPIPTPFVIEEMSRLFPCIEEEKLEKMI 240
           TYSVSKVAVIGIVKSLASELC+YGIRVNCISPFPIPTPFVIEEM +LFP +EE KLEKMI
Sbjct: 181 TYSVSKVAVIGIVKSLASELCRYGIRVNCISPFPIPTPFVIEEMVQLFPRVEEAKLEKMI 240

Query: 241 FDLSGLEGTVCETNDIANAALYLASDDAKYVSGHNLVVDGAFTCFKSLNFPLPDQNSIQG 300
           FDLS LEGTVCETNDIANAAL+LASDDAKYVSGHNLVVDGAFTCFKSLNFPLPDQN+I G
Sbjct: 241 FDLSALEGTVCETNDIANAALFLASDDAKYVSGHNLVVDGAFTCFKSLNFPLPDQNTIHG 300

Query: 301 DS 302
           +S
Sbjct: 301 ES 302

[Close]

BLAST of Lsi09G005530 vs. NCBI nr
Match: XP_023534149.1 (secoisolariciresinol dehydrogenase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 502.7 bits (1293), Expect = 2.2e-138
Identity = 255/288 (88.54%), Postives = 268/288 (93.06%), Query Frame = 0


Query: 8   YLFNNSLNSSLFFNRGLSSQAGRLEGKIALITGAASGIGKATAEKFISNGAKVVIADINE 67
           +L  NSL  S FFNRGLSS AGRLEGKIALITGAASGIGKATA KFISNGAKVV+AD+ E
Sbjct: 5   HLLRNSLTLS-FFNRGLSSHAGRLEGKIALITGAASGIGKATASKFISNGAKVVLADVEE 64

Query: 68  KLGQHTAKHLGPNATFIPCDVTKESNVSDAVDFTLSLHKKLDIMYNNAGIACNTPPTILD 127
           +LGQ TAK LGPNATFIPCDV+ E+NVSDAVDF LSLHK+LDIMYNNAGIACNTPP+ILD
Sbjct: 65  ELGQATAKQLGPNATFIPCDVSNEANVSDAVDFALSLHKRLDIMYNNAGIACNTPPSILD 124

Query: 128 LDLAVFDKVMNINVRGVLAGIKHASRVMIPRRSGSILCTASVTGLMGGLAQHTYSVSKVA 187
           LDLAVFDKVMNINVRGVLAGIKHASRVMIP++SGSILCTASVTG+MGGLAQHTYSVSKVA
Sbjct: 125 LDLAVFDKVMNINVRGVLAGIKHASRVMIPQQSGSILCTASVTGVMGGLAQHTYSVSKVA 184

Query: 188 VIGIVKSLASELCKYGIRVNCISPFPIPTPFVIEEMSRLFPCIEEEKLEKMIFDLSGLEG 247
           VIGIVKS+ASELCKYGIRVNCISPFPIPTPFVI EM RLFP  E+EKLEKMIFDLS LEG
Sbjct: 185 VIGIVKSMASELCKYGIRVNCISPFPIPTPFVINEMCRLFPGTEKEKLEKMIFDLSALEG 244

Query: 248 TVCETNDIANAALYLASDDAKYVSGHNLVVDGAFTCFKSLNFPLPDQN 296
            VCETNDIANAALYLASDDAKYVSGHNLVVDG FTCFK LNFPLPDQN
Sbjct: 245 AVCETNDIANAALYLASDDAKYVSGHNLVVDGGFTCFKRLNFPLPDQN 291

[Close]

BLAST of Lsi09G005530 vs. NCBI nr
Match: TYK20720.1 (secoisolariciresinol dehydrogenase [Cucumis melo var. makuwa])

HSP 1 Score: 501.5 bits (1290), Expect = 5.0e-138
Identity = 252/271 (92.99%), Postives = 262/271 (96.68%), Query Frame = 0


Query: 31  LEGKIALITGAASGIGKATAEKFISNGAKVVIADINEKLGQHTAKHLGPNATFIPCDVTK 90
           LEGKIALITGAASGIGKATA KFISNGAKVVIADI EKLGQ TAK LGPNATFI CDVTK
Sbjct: 5   LEGKIALITGAASGIGKATAGKFISNGAKVVIADIKEKLGQDTAKQLGPNATFIHCDVTK 64

Query: 91  ESNVSDAVDFTLSLHKKLDIMYNNAGIACNTPPTILDLDLAVFDKVMNINVRGVLAGIKH 150
           ESN+SDAVDFT+SLHKKLDIMYNNAGIACNTPP+I DLDLAVFDKVMN+NVRGVLAGIKH
Sbjct: 65  ESNISDAVDFTISLHKKLDIMYNNAGIACNTPPSISDLDLAVFDKVMNVNVRGVLAGIKH 124

Query: 151 ASRVMIPRRSGSILCTASVTGLMGGLAQHTYSVSKVAVIGIVKSLASELCKYGIRVNCIS 210
           ASRVMIPRRSGSILCTASVTGLMGGLAQHTYSVSKVAVIGIVKSLASELC+YGIRVNCIS
Sbjct: 125 ASRVMIPRRSGSILCTASVTGLMGGLAQHTYSVSKVAVIGIVKSLASELCRYGIRVNCIS 184

Query: 211 PFPIPTPFVIEEMSRLFPCIEEEKLEKMIFDLSGLEGTVCETNDIANAALYLASDDAKYV 270
           PFPIPTPFVIEEM++LFP +EEEKLEKMIFDLS LEGTVCETNDIANAALYLASDDAKYV
Sbjct: 185 PFPIPTPFVIEEMTQLFPSVEEEKLEKMIFDLSALEGTVCETNDIANAALYLASDDAKYV 244

Query: 271 SGHNLVVDGAFTCFKSLNFPLPDQNSIQGDS 302
           SGHNLVVDGAFTCFKSLNF LPDQN+IQG+S
Sbjct: 245 SGHNLVVDGAFTCFKSLNFSLPDQNTIQGES 275

[Close]

BLAST of Lsi09G005530 vs. TAIR 10
Match: AT4G03140.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 380.6 bits (976), Expect = 1.2e-105
Identity = 192/300 (64.00%), Postives = 237/300 (79.00%), Query Frame = 0


Query: 1   MIQIRLRYLFNNSLNSSLFFNRGLSSQAG------RLEGKIALITGAASGIGKATAEKFI 60
           M QI    LF N   S  F  +G+SS +       +LEGK+ALITG ASGIGKATA KFI
Sbjct: 44  MFQIGKNALFKNV--SKNFLIKGISSSSSSHSTSRKLEGKVALITGGASGIGKATAGKFI 103

Query: 61  SNGAKVVIADINEKLGQHTAKHLGPNATFIPCDVTKESNVSDAVDFTLSLHKKLDIMYNN 120
           S+GAKV+IADI  ++G+ T + LGP+  + PCDVTKES++++AVDF +SLH KLDIMYNN
Sbjct: 104 SHGAKVIIADIQPQIGRETEQELGPSCAYFPCDVTKESDIANAVDFAVSLHTKLDIMYNN 163

Query: 121 AGIACNTPPTILDLDLAVFDKVMNINVRGVLAGIKHASRVMIPRRSGSILCTASVTGLMG 180
           AGI C TPP+I+DLDL VFDKV+N NVRGV+AGIKHA+RVMIPR SGSI+C  SVTG+MG
Sbjct: 164 AGIPCKTPPSIVDLDLNVFDKVINTNVRGVMAGIKHAARVMIPRNSGSIICAGSVTGMMG 223

Query: 181 GLAQHTYSVSKVAVIGIVKSLASELCKYGIRVNCISPFPIPTPFVIEEMSRLFPCIEEEK 240
           GLAQHTYSVSK AVIGIV+S ASELCK+ IRVNCISPF I T FV++EM +++P +++ +
Sbjct: 224 GLAQHTYSVSKSAVIGIVRSTASELCKHRIRVNCISPFAITTSFVMDEMRQIYPGVDDSR 283

Query: 241 LEKMIFDLSGLEGTVCETNDIANAALYLASDDAKYVSGHNLVVDGAFTCFKSLNFPLPDQ 295
           L +++     L G VCE  D+ANAA+YLASDD+KYV+GHNLVVDG FT  K+L+FP PDQ
Sbjct: 284 LIQIVQSTGVLNGEVCEPTDVANAAVYLASDDSKYVNGHNLVVDGGFTTVKTLDFPAPDQ 341

[Close]

BLAST of Lsi09G005530 vs. TAIR 10
Match: AT3G26760.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 308.1 bits (788), Expect = 7.6e-84
Identity = 155/285 (54.39%), Postives = 207/285 (72.63%), Query Frame = 0


Query: 9   LFNNSLNSSLFFNRGLSSQAGRLEGKIALITGAASGIGKATAEKFISNGAKVVIADINEK 68
           L +  + S+L +    S+ + +LEGK+A+ITG ASGIGKATAE+F+S GA+V+I DI+E+
Sbjct: 18  LISKPIRSTLLY----STSSRKLEGKVAVITGGASGIGKATAEEFVSQGAQVIIVDIDEE 77

Query: 69  LGQHTAKHLGPNATFIPCDVTKESNVSDAVDFTLSLHKKLDIMYNNAGIACN-TPPTILD 128
            G   A  LG  A F+ CDVT+E  ++ AV+  ++ H KLD+M N+AGI+C+ +PP+I D
Sbjct: 78  AGHMVATELGSAAHFLRCDVTEEEQIAKAVETAVTRHGKLDVMLNSAGISCSISPPSIAD 137

Query: 129 LDLAVFDKVMNINVRGVLAGIKHASRVMIPRRSGSILCTASVTGLMGGLAQHTYSVSKVA 188
           LD+  +DKVM +NVRG + GIKHA+R MIP  SGSILC +S++GLMGGL  H YS+SK  
Sbjct: 138 LDMDTYDKVMRLNVRGTVLGIKHAARAMIPAGSGSILCLSSISGLMGGLGPHAYSISKFT 197

Query: 189 VIGIVKSLASELCKYGIRVNCISPFPIPTPFVIEEMSRLFP--CIEEEKLEKMIFDLSGL 248
           + G+VK++ASELCK+G+R+NCISP  IPTP  +      F    I EE+L  ++     L
Sbjct: 198 IPGVVKTVASELCKHGLRINCISPAGIPTPLTLRMFREAFAGHSIREEQLLAIVNATGEL 257

Query: 249 EGTVCETNDIANAALYLASDDAKYVSGHNLVVDGAFTCFKSLNFP 291
           +G  CE  D+A AALYLASDDAK+V+GHNLVVDG FTCFKSLN P
Sbjct: 258 KGEKCEEIDVAKAALYLASDDAKFVTGHNLVVDGGFTCFKSLNLP 298

[Close]

BLAST of Lsi09G005530 vs. TAIR 10
Match: AT3G26770.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 301.6 bits (771), Expect = 7.1e-82
Identity = 147/274 (53.65%), Postives = 199/274 (72.63%), Query Frame = 0


Query: 23  GLSSQAGRLEGKIALITGAASGIGKATAEKFISNGAKVVIADINEKLGQHTAKHLGPNAT 82
           G +  + +LEGK+ALITG ASG+GKATA +F+ +GA+VVIAD++ + G  TAK LG  A 
Sbjct: 33  GCTCTSKKLEGKVALITGGASGLGKATASEFLRHGARVVIADLDAETGTKTAKELGSEAE 92

Query: 83  FIPCDVTKESNVSDAVDFTLSLHKKLDIMYNNAGI-ACNTPPTILDLDLAVFDKVMNINV 142
           F+ CDVT E++++ AV+ T+  + KLD+MYNNAGI    TP +I  LD+  F++VM INV
Sbjct: 93  FVRCDVTVEADIAGAVEMTVERYGKLDVMYNNAGIVGPMTPASISQLDMTEFERVMRINV 152

Query: 143 RGVLAGIKHASRVMIPRRSGSILCTASVTGLMGGLAQHTYSVSKVAVIGIVKSLASELCK 202
            GV++GIKHA++ MIP RSG ILCT+SV G+ GGLA H+Y++SK    GIVKS ASELC+
Sbjct: 153 FGVVSGIKHAAKFMIPARSGCILCTSSVAGVTGGLAPHSYTISKFTTPGIVKSAASELCE 212

Query: 203 YGIRVNCISPFPIPTPFVIEEMSRLFPCIEEEKLEKMIFDLSGLEGTVCETNDIANAALY 262
           +G+R+NCISP  + TP  +  + ++FP + EEKL + +  +  L+G  CE  D+A AALY
Sbjct: 213 HGVRINCISPGTVATPLTLSYLQKVFPKVSEEKLRETVKGMGELKGAECEEADVAKAALY 272

Query: 263 LASDDAKYVSGHNLVVDGAFTCFKSLNFPLPDQN 296
           LAS+D KYV+GHNLVVDG  T FK   FP P  +
Sbjct: 273 LASNDGKYVTGHNLVVDGGMTAFKIAGFPFPSDS 306

[Close]

BLAST of Lsi09G005530 vs. TAIR 10
Match: AT3G51680.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 230.3 bits (586), Expect = 2.0e-60
Identity = 128/265 (48.30%), Postives = 171/265 (64.53%), Query Frame = 0


Query: 30  RLEGKIALITGAASGIGKATAEKFISNGAKVVIADINEKLGQHTAKHL-----GPNATFI 89
           RLEGK+A+ITG A GIGKAT   F  +GA VVIAD++   G   AK L      P   FI
Sbjct: 31  RLEGKVAIITGGAHGIGKATVMLFARHGATVVIADVDNVAGSSLAKSLSSHKTSPMVAFI 90

Query: 90  PCDVTKESNVSDAVDFTLSLHKKLDIMYNNAGIACNTP--PTILDLDLAVFDKVMNINVR 149
            CDV+ E++V + V+ T++ + +LDI++NNAG+  +     +ILD D   FD VM +NVR
Sbjct: 91  SCDVSVEADVENLVNVTVARYGRLDILFNNAGVLGDQKKHKSILDFDADEFDHVMRVNVR 150

Query: 150 GVLAGIKHASRVMIPRR-SGSILCTASVTGLMGGLAQHTYSVSKVAVIGIVKSLASELCK 209
           GV  G+KH +R MI R   G I+ TASV G+MGG+  H Y+ SK A++G+ K+ A EL K
Sbjct: 151 GVGLGMKHGARAMIKRGFKGCIISTASVAGVMGGMGPHAYTASKHAIVGLTKNAACELGK 210

Query: 210 YGIRVNCISPFPIPTPFVIEEMSRLFPCIEE----EKLEKMIFDLSGLEGTVCETNDIAN 269
           YGIRVNCISPF + T  ++    +      E    E++E+ +  L+ L+G     NDIA 
Sbjct: 211 YGIRVNCISPFGVATSMLVNAWRKTSGGDVEDDDVEEMEEFVRSLANLKGETLRANDIAE 270

Query: 270 AALYLASDDAKYVSGHNLVVDGAFT 283
           AALYLASD++KYV+GHNLVVDG  T
Sbjct: 271 AALYLASDESKYVNGHNLVVDGGVT 295

[Close]

BLAST of Lsi09G005530 vs. TAIR 10
Match: AT2G47120.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 213.0 bits (541), Expect = 3.3e-55
Identity = 119/259 (45.95%), Postives = 168/259 (64.86%), Query Frame = 0


Query: 30  RLEGKIALITGAASGIGKATAEKFISNGAKVVIADINEKLGQHTAKHLGPN-ATFIPCDV 89
           RLEGKI +ITG ASGIG   A  F  +GAKVVI D+ E+LGQ+ A  +G + A+F  CDV
Sbjct: 5   RLEGKIVIITGGASGIGADAARLFTDHGAKVVIVDVQEELGQNVAVLIGKDKASFYRCDV 64

Query: 90  TKESNVSDAVDFTLSLHKKLDIMYNNAGIACNTPPTILDLDLAVFDKVMNINVRGVLAGI 149
           T E+ V DAV FT+  H KLD++++NAG+      + LD DL  FD++M +NVRG  A I
Sbjct: 65  TNETEVEDAVKFTVEKHGKLDVLFSNAGV-LEPLESFLDFDLERFDRIMAVNVRGAAAFI 124

Query: 150 KHASRVMIPRRS-GSILCTASVTGLMGGLAQHTYSVSKVAVIGIVKSLASELCKYGIRVN 209
           KHA+R M+ + + GSI+CT SV+  +GG   H Y+ SK  ++G+++S   +L KYGIRVN
Sbjct: 125 KHAARAMVEKGTRGSIVCTTSVSAEIGG-GHHGYTASKHGLVGLIRSACGDLGKYGIRVN 184

Query: 210 CISPFPIPTPFVIEEMSRLFPCIEEEKLEKMIFDLSG-LEGTVCETNDIANAALYLASDD 269
            ++P+ + TP    +       +  ++LE   FD  G L+G V + + +A  AL+LASDD
Sbjct: 185 GVAPYAVATPMTSHDE------VTGKQLED-YFDAKGILKGMVLKASHVAQVALFLASDD 244

Query: 270 AKYVSGHNLVVDGAFTCFK 286
           + Y+SG NL VDG +T  K
Sbjct: 245 SAYISGQNLAVDGGYTVVK 254

The following BLAST results are available for this feature:

BLAST of Lsi09G005530 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94KL7	2.6e-65	50.36	Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 P...	[more]
Q9SCU0	2.8e-59	48.30	Short-chain dehydrogenase reductase 2a OS=Arabidopsis thaliana OX=3702 GN=SDR2a ...	[more]
P50160	1.1e-58	43.26	Sex determination protein tasselseed-2 OS=Zea mays OX=4577 GN=TS2 PE=2 SV=1	[more]
F1SWA0	7.7e-57	45.80	Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1	[more]
H9BFQ0	1.3e-56	48.05	Tropinone reductase-like 1 OS=Erythroxylum coca OX=289672 PE=2 SV=1	[more]

BLAST of Lsi09G005530 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CT49	2.0e-148	91.06	secoisolariciresinol dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103504658 PE=4 ...	[more]
A0A0A0KL23	3.2e-146	89.40	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G171720 PE=4 SV=1	[more]
A0A5D3DB97	2.4e-138	92.99	Secoisolariciresinol dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A5A7UNV0	1.3e-136	92.91	Secoisolariciresinol dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C...	[more]
A0A6J1K592	5.0e-136	87.50	secoisolariciresinol dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111491301 P...	[more]

BLAST of Lsi09G005530 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038876474.1	1.3e-149	92.05	secoisolariciresinol dehydrogenase [Benincasa hispida]	[more]
XP_008467261.1	4.1e-148	91.06	PREDICTED: secoisolariciresinol dehydrogenase [Cucumis melo]	[more]
XP_004143649.1	6.5e-146	89.40	secoisolariciresinol dehydrogenase [Cucumis sativus] >KGN50390.1 hypothetical pr...	[more]
XP_023534149.1	2.2e-138	88.54	secoisolariciresinol dehydrogenase [Cucurbita pepo subsp. pepo]	[more]
TYK20720.1	5.0e-138	92.99	secoisolariciresinol dehydrogenase [Cucumis melo var. makuwa]	[more]

BLAST of Lsi09G005530 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G03140.1	1.2e-105	64.00	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G26760.1	7.6e-84	54.39	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G26770.1	7.1e-82	53.65	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G51680.1	2.0e-60	48.30	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G47120.1	3.3e-55	45.95	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 35..52 score: 50.17 coord: 106..117 score: 50.13 coord: 155..171 score: 32.07 coord: 245..265 score: 29.71 coord: 181..200 score: 39.49 coord: 202..219 score: 39.71
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00080	SDRFAMILY	coord: 106..117 score: 48.31 coord: 161..169 score: 41.95 coord: 181..200 score: 38.35
None	No IPR available	GENE3D	3.40.50.720		coord: 21..288 e-value: 1.6E-76 score: 258.9
None	No IPR available	PFAM	PF13561	adh_short_C2	coord: 40..281 e-value: 1.9E-61 score: 207.7
None	No IPR available	PIRSR	PIRSR000094-3	PIRSR000094-3	coord: 29..283 e-value: 3.3E-18 score: 63.6
None	No IPR available	PIRSR	PIRSR000095-1	PIRSR000095-1	coord: 37..221 e-value: 2.1E-25 score: 87.3
None	No IPR available	PIRSR	PIRSR000094-2	PIRSR000094-2	coord: 29..283 e-value: 3.3E-18 score: 63.6
None	No IPR available	PANTHER	PTHR43180:SF55	ALCOHOL DEHYDROGENASE-LIKE PROTEIN	coord: 14..293
None	No IPR available	PANTHER	PTHR43180	3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_6G11210)	coord: 14..293
IPR020904	Short-chain dehydrogenase/reductase, conserved site	PROSITE	PS00061	ADH_SHORT	coord: 168..196
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 32..282

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi09G005530.1	Lsi09G005530.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016491	oxidoreductase activity