Lsi09G004170 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi09G004170
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	L-lactate dehydrogenase
Location	chr09: 4441686 .. 4444057 (-)
RNA-Seq Expression	Lsi09G004170
Synteny	Lsi09G004170

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAGAAGAGTTCATCAACATCTTCTTTAGGTCCAGGCGGCCTTGACCTTTCTCAAGCCTTCTTCAAACCAATCCGCGACGCCTCTCCCCCATCGACTTCAAAGCGCAACTCCAAAATCTCCGTCATCGGCGCCGGGAACGTCGGCATGGCCATCGCTCAGACCATCCTCACTCAAGATCTTGTCGAAGAACTCGTCCTCGTCGACGCCAAGCCCGACAAACTCCGCGGCGAAATGCTCGATCTCCAACACGCTGCCGCCTTCCTCCCCCGAACCAAAATCAGCGCCTCCACCGATTACTCCATTACCGCTGGATCCGATCTATGTATCGTCACCGCTGGAGCTCGCCAGATCGCTGGTGAATCTAGGTTGAATCTGCTGCAGAGGAACGTTGCTCTGTTTCAGAAGATCGTGCCGCCGCTGGTTCAGTTCTCTCCCGAGACGATTCTTTTGATTGTTTCGAATCCGGTGGATGTTTTGACTTATGTTGCTTGGAAGCTCTCTGGTTTTCCGTCTAATCGAGTGATTGGATCCGGAACGAATCTGGATTCGTCTAGGTTTCGGTTTCTCATCGCCGATCATCTGGACGTCAATGCTCAGGATGTTCAGGTCAGTTTTCTGATTGACATTTCTTCTACCTTCTTCAATCAATCAGTGTTTATTTGTTAATTTGGTGTTGCTTTTTGAAGAAATTTCAATTTAGGGTTTGAAACCTTTCGATTTTTTCGTTTTTGGTTGATTACTTGATTAGTATCAAATGTGGCTGATAATTTTAGGGAAAATTTCGAACATTTCCCTAGAATTTGGAAGTTGGCAACTCGCCAACAAATCACAATAACTATTTGAAATATCTATCCGGAATATATGTTATGTGGATTAAATCTTATTTTCAACTTTCATATTTATTTAATTTTGATTTCTTAACTTTTTTGATGTTTATTTTAATCATTGAATTTTTTAATAATAAAACCTAAATTTTTTTTGGTTAAATTATAAGTTTAGTACTTTCATTTTTATGTTTGTGATTAACACGTCTTTGAACATTCAATTATATTTCTAATAGGTTTTTAACCTTTAAAATCATCGAATAGATCATTGAACTTTCAGATTTATGTCTAATAGATTATAATATAAATATATAATTTTAAAATGAACAAATCAATTAGCCATAATATAAAATTGATTCAATTACAAATTTAGTTCATAAACTTTGTGACTTCTGTCTATTTGACCTTTGAACTTTGAAATTACAAACTCAGTTTGAATTTTTCTTATATATACTTTTAAATTTCACGAATCTGCTAGACATAAAACTGAAAATTTGTAGTTTAATTAGACATAAAATTCAAATTTATATCAAATAGATTGAAATTTTATGAGCCTATTAGACACAAAAGTAAAAGTTTAGAGATCTATTAGACAATTTCAAAAAATCTTGAATAGGTTTAAGAACTATTTTACTTAAAACTAAATATGTAAGTTTATTAAACACAAAATCTAAAGTTTAGAGAGTTATTAGACACTTTTTAAAGTTGATAAATCTATTAAAATTGAGAGACTTATTTACCACAAACCAAAAAGTACATAGTCTAAACTTAGTAATTTAACTAAAAATGTAAAAATAACCAATTTTCTACCTAATATTTCTTCCCCAATACTCACCTCTGTTAAACTTGTTAATAGCAAAACAAAGATAAAGGACAATTATAGTTATTACTTTTAATGTTGAAGGAGAAAAATTAGAAAAAGAAGAAACAATAAATTCAAAGAATGAAAAAATAAATTCAAAGTTGACCCAAGTAGAATTCAAACCATCATATAGGTGCCTAACCTATGCATCAAACTATAGATGAATTTCTATAAATCAAACTAGCATTGATATTTAAAAGATGAAGCTAAAAAAAAGAGGATGATATATATGTTTGAAGGCATACATAGTGGGAGAGCATGGCGACAGCTCGGTGGCGCTATGGTCAAGCATAAGTGTGGGCGGGGTGCCAATACTGAGCTTTCTAAAGAATCAACAAATTGCCTACGAAAAAGAAACATTAGAGAAAATTCACAAAGAGGTCGTAGGCGGGGCCTATGAAGTCATAAGCCTGAAGGGCTACACATCATGGGCGATTGGGTACTCCGTGGCGAGCTTGGCTCGGTCGCTCCTTCGTGACCAACGGAGGATCCACCCCGTCTCTGTCCTTGCAAAAGGGTTCTACGGCATTGATGGTGGCGAGTTGTTCCTCAGCCTGCCTGCACAGCTCGGGAGGGGCGGAGTATTGGGGATCACCAATGTGCATCTAACGGCGGAGGAGTCGAGACGTTTGAGGGACTCGGCGAACACCATCTTGCAGGTGCAGACTGAATTGGGGATCTGA

mRNA sequence

ATGCAGAAGAGTTCATCAACATCTTCTTTAGGTCCAGGCGGCCTTGACCTTTCTCAAGCCTTCTTCAAACCAATCCGCGACGCCTCTCCCCCATCGACTTCAAAGCGCAACTCCAAAATCTCCGTCATCGGCGCCGGGAACGTCGGCATGGCCATCGCTCAGACCATCCTCACTCAAGATCTTGTCGAAGAACTCGTCCTCGTCGACGCCAAGCCCGACAAACTCCGCGGCGAAATGCTCGATCTCCAACACGCTGCCGCCTTCCTCCCCCGAACCAAAATCAGCGCCTCCACCGATTACTCCATTACCGCTGGATCCGATCTATGTATCGTCACCGCTGGAGCTCGCCAGATCGCTGGTGAATCTAGGTTGAATCTGCTGCAGAGGAACGTTGCTCTGTTTCAGAAGATCGTGCCGCCGCTGGTTCAGTTCTCTCCCGAGACGATTCTTTTGATTGTTTCGAATCCGGTGGATGTTTTGACTTATGTTGCTTGGAAGCTCTCTGGTTTTCCGTCTAATCGAGTGATTGGATCCGGAACGAATCTGGATTCGTCTAGGTTTCGGTTTCTCATCGCCGATCATCTGGACGTCAATGCTCAGGATGTTCAGGCATACATAGTGGGAGAGCATGGCGACAGCTCGGTGGCGCTATGGTCAAGCATAAGTGTGGGCGGGGTGCCAATACTGAGCTTTCTAAAGAATCAACAAATTGCCTACGAAAAAGAAACATTAGAGAAAATTCACAAAGAGGTCGTAGGCGGGGCCTATGAAGTCATAAGCCTGAAGGGCTACACATCATGGGCGATTGGGTACTCCGTGGCGAGCTTGGCTCGGTCGCTCCTTCGTGACCAACGGAGGATCCACCCCGTCTCTGTCCTTGCAAAAGGGTTCTACGGCATTGATGGTGGCGAGTTGTTCCTCAGCCTGCCTGCACAGCTCGGGAGGGGCGGAGTATTGGGGATCACCAATGTGCATCTAACGGCGGAGGAGTCGAGACGTTTGAGGGACTCGGCGAACACCATCTTGCAGGTGCAGACTGAATTGGGGATCTGA

Coding sequence (CDS)

ATGCAGAAGAGTTCATCAACATCTTCTTTAGGTCCAGGCGGCCTTGACCTTTCTCAAGCCTTCTTCAAACCAATCCGCGACGCCTCTCCCCCATCGACTTCAAAGCGCAACTCCAAAATCTCCGTCATCGGCGCCGGGAACGTCGGCATGGCCATCGCTCAGACCATCCTCACTCAAGATCTTGTCGAAGAACTCGTCCTCGTCGACGCCAAGCCCGACAAACTCCGCGGCGAAATGCTCGATCTCCAACACGCTGCCGCCTTCCTCCCCCGAACCAAAATCAGCGCCTCCACCGATTACTCCATTACCGCTGGATCCGATCTATGTATCGTCACCGCTGGAGCTCGCCAGATCGCTGGTGAATCTAGGTTGAATCTGCTGCAGAGGAACGTTGCTCTGTTTCAGAAGATCGTGCCGCCGCTGGTTCAGTTCTCTCCCGAGACGATTCTTTTGATTGTTTCGAATCCGGTGGATGTTTTGACTTATGTTGCTTGGAAGCTCTCTGGTTTTCCGTCTAATCGAGTGATTGGATCCGGAACGAATCTGGATTCGTCTAGGTTTCGGTTTCTCATCGCCGATCATCTGGACGTCAATGCTCAGGATGTTCAGGCATACATAGTGGGAGAGCATGGCGACAGCTCGGTGGCGCTATGGTCAAGCATAAGTGTGGGCGGGGTGCCAATACTGAGCTTTCTAAAGAATCAACAAATTGCCTACGAAAAAGAAACATTAGAGAAAATTCACAAAGAGGTCGTAGGCGGGGCCTATGAAGTCATAAGCCTGAAGGGCTACACATCATGGGCGATTGGGTACTCCGTGGCGAGCTTGGCTCGGTCGCTCCTTCGTGACCAACGGAGGATCCACCCCGTCTCTGTCCTTGCAAAAGGGTTCTACGGCATTGATGGTGGCGAGTTGTTCCTCAGCCTGCCTGCACAGCTCGGGAGGGGCGGAGTATTGGGGATCACCAATGTGCATCTAACGGCGGAGGAGTCGAGACGTTTGAGGGACTCGGCGAACACCATCTTGCAGGTGCAGACTGAATTGGGGATCTGA

Protein sequence

MQKSSSTSSLGPGGLDLSQAFFKPIRDASPPSTSKRNSKISVIGAGNVGMAIAQTILTQDLVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAGESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGTNLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYEKETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGIDGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDSANTILQVQTELGI

Homology

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BLAST of Lsi09G004170 vs. ExPASy Swiss-Prot
Match: P22988 (L-lactate dehydrogenase A OS=Hordeum vulgare OX=4513 PE=1 SV=1)

HSP 1 Score: 461.5 bits (1186), Expect = 8.7e-129
Identity = 243/348 (69.83%), Postives = 287/348 (82.47%), Query Frame = 0


Query: 1   MQKSSSTSSLGPGGLDLSQAFFKPIRDASP--PSTS----KRNSKISVIGAGNVGMAIAQ 60
           M K+SS S LG      S  FF+P+ D  P  P++S    +R +KISVIGAGNVGMAIAQ
Sbjct: 1   MHKASSLSELGFDAGGASSGFFRPVADGCPATPTSSAVPHRRLTKISVIGAGNVGMAIAQ 60

Query: 61  TILTQDLVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAG 120
           TILTQ+L +E+ LVDA PDKLRGE LDLQHAAAFLPR +IS  TD ++T  SDL IVTAG
Sbjct: 61  TILTQNLADEIALVDALPDKLRGEALDLQHAAAFLPRVRIS-GTDAAVTKNSDLVIVTAG 120

Query: 121 ARQIAGESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNR 180
           ARQI GE+RLNLLQRNVAL++KIVPP+ + SP+ +LL+VSNPVDVLTYVAWKLSGFP++R
Sbjct: 121 ARQIPGETRLNLLQRNVALYRKIVPPVAEHSPDALLLVVSNPVDVLTYVAWKLSGFPASR 180

Query: 181 VIGSGTNLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKN 240
           VIGSGTNLDSSRFRFLIA+HLDVNAQDVQAY+VGEHGDSSVA+WSSISVGG+P    L++
Sbjct: 181 VIGSGTNLDSSRFRFLIAEHLDVNAQDVQAYMVGEHGDSSVAIWSSISVGGMPAFKSLRD 240

Query: 241 QQIAYEKETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLA 300
              ++++  LE I + VVGGAYEVI LKGYTSWAIGYSVASLA SLLRDQRR+HPVSVLA
Sbjct: 241 SHRSFDEAALEGIRRAVVGGAYEVIGLKGYTSWAIGYSVASLAASLLRDQRRVHPVSVLA 300

Query: 301 KGFYGI-DGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDSANTI 342
            GF+GI DG E+FLSLPA+LGRGG+LG+  + LT  E+ +LR SA T+
Sbjct: 301 SGFHGISDGHEVFLSLPARLGRGGILGVAEMDLTEAEAAQLRRSAKTL 347

[Close]

BLAST of Lsi09G004170 vs. ExPASy Swiss-Prot
Match: P22989 (L-lactate dehydrogenase B (Fragment) OS=Hordeum vulgare OX=4513 PE=1 SV=1)

HSP 1 Score: 451.1 bits (1159), Expect = 1.2e-125
Identity = 232/342 (67.84%), Postives = 280/342 (81.87%), Query Frame = 0


Query: 16  DLSQAFFKPIRDASP------PSTSKRNSKISVIGAGNVGMAIAQTILTQDLVEELVLVD 75
           D S  FF+ + D  P       +  +R +K+SVIGAGNVGMAIAQTILTQ+L +E+ LVD
Sbjct: 3   DASSGFFRAVADGCPITHTSCSAPHRRLTKVSVIGAGNVGMAIAQTILTQNLADEIALVD 62

Query: 76  AKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAGESRLNLLQR 135
           A PDKLRGE LDLQHAAAFLPR +I + TD ++T  SDL +VTAGARQI GE+RLNLLQR
Sbjct: 63  ALPDKLRGEALDLQHAAAFLPRVRIVSGTDAAVTKNSDLIVVTAGARQIPGETRLNLLQR 122

Query: 136 NVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGTNLDSSRFRF 195
           NVAL++KIVPP+ + SP+ +LL+VSNPVDVLTYVAWKLSGFP++RVIGSGTNLDSSRFRF
Sbjct: 123 NVALYRKIVPPVAEHSPDALLLVVSNPVDVLTYVAWKLSGFPASRVIGSGTNLDSSRFRF 182

Query: 196 LIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYEKETLEKIHK 255
           L+A+HLDV+AQDVQAY+VGEHGDSSVA+WSSISVGG+P L  L++   ++++  LE I +
Sbjct: 183 LVAEHLDVSAQDVQAYMVGEHGDSSVAIWSSISVGGMPALKSLRDSHRSFDEAALEGIRR 242

Query: 256 EVVGGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI-DGGELFLS 315
            VVGGAYEVI LKGYTSWAIGYSVASLA SLLRDQRR+HPVSVLA GF+GI DG E+FLS
Sbjct: 243 AVVGGAYEVIGLKGYTSWAIGYSVASLATSLLRDQRRVHPVSVLAAGFHGISDGHEVFLS 302

Query: 316 LPAQLGRGGVLGITNVHLTAEESRRLRDSANTILQVQTELGI 351
           LPA+LGR GVLG+  + LT  E+ +LR SA T+ +    LG+
Sbjct: 303 LPARLGRAGVLGVAEMDLTEAEAAQLRRSAKTLWENCQLLGL 344

[Close]

BLAST of Lsi09G004170 vs. ExPASy Swiss-Prot
Match: P29038 (L-lactate dehydrogenase OS=Zea mays OX=4577 PE=2 SV=1)

HSP 1 Score: 436.4 bits (1121), Expect = 3.0e-121
Identity = 236/355 (66.48%), Postives = 284/355 (80.00%), Query Frame = 0


Query: 1   MQKSSSTSSLGPGGLDLSQAFFKPIR-DASPPSTS---KRNSKISVIGAGNVGMAIAQTI 60
           M+K++S S LG    D S  FF+P+  D+S P++    +R +K+SVIGAGNVGMAIAQTI
Sbjct: 1   MKKATSLSELGFDAGDASSGFFRPVSGDSSTPTSQHHRRRLTKVSVIGAGNVGMAIAQTI 60

Query: 61  LTQDLVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGAR 120
           LT+DL +E+ LVDA PDKLRGEMLDLQHAAAFLPRT++ + TD S+T GSDL IVTAGAR
Sbjct: 61  LTRDLADEIALVDAVPDKLRGEMLDLQHAAAFLPRTRLVSGTDMSVTRGSDLVIVTAGAR 120

Query: 121 QIAGESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVI 180
           QI GE+RL+LLQRNVALF+KIVPPL + S + +LL+VSNPVDVLTYVAWKLSGFP++RVI
Sbjct: 121 QIQGETRLDLLQRNVALFRKIVPPLAEQSHDALLLVVSNPVDVLTYVAWKLSGFPASRVI 180

Query: 181 GSGTNLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQ 240
           GSGTNLDSSRFRFL+A+HLDVNAQDVQAY+VGEHGDSSVA+WSS+SV G+P+L  L+   
Sbjct: 181 GSGTNLDSSRFRFLLAEHLDVNAQDVQAYMVGEHGDSSVAVWSSVSVAGMPVLKSLQESH 240

Query: 241 IAYEKETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKG 300
             +++E LE I + VV  AYEVISLKGYTSWAIGYSVASLA SLLRDQRRIHPVSVLA+G
Sbjct: 241 RCFDEEALEGIRRAVVDSAYEVISLKGYTSWAIGYSVASLAASLLRDQRRIHPVSVLARG 300

Query: 301 FYGI-DGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDSANTILQVQTELGI 351
           F+GI DG     + P +  R    G   + LT EE++RLR SA TI +    LG+
Sbjct: 301 FHGIPDGTTSSSACPPRRPRRRP-GRREMELTEEEAKRLRRSAKTIWENCQLLGL 354

[Close]

BLAST of Lsi09G004170 vs. ExPASy Swiss-Prot
Match: P06151 (L-lactate dehydrogenase A chain OS=Mus musculus OX=10090 GN=Ldha PE=1 SV=3)

HSP 1 Score: 342.4 bits (877), Expect = 5.9e-93
Identity = 173/311 (55.63%), Postives = 229/311 (73.63%), Query Frame = 0


Query: 38  SKISVIGAGNVGMAIAQTILTQDLVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISAS 97
           +KI+V+G G VGMA A +IL +DL +EL LVD   DKL+GEM+DLQH + FL   KI +S
Sbjct: 21  NKITVVGVGAVGMACAISILMKDLADELALVDVMEDKLKGEMMDLQHGSLFLKTPKIVSS 80

Query: 98  TDYSITAGSDLCIVTAGARQIAGESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPV 157
            DY +TA S L I+TAGARQ  GESRLNL+QRNV +F+ I+P +V++SP   LLIVSNPV
Sbjct: 81  KDYCVTANSKLVIITAGARQQEGESRLNLVQRNVNIFKFIIPNIVKYSPHCKLLIVSNPV 140

Query: 158 DVLTYVAWKLSGFPSNRVIGSGTNLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVAL 217
           D+LTYVAWK+SGFP NRVIGSG NLDS+RFR+L+ + L V+A     +++GEHGDSSV +
Sbjct: 141 DILTYVAWKISGFPKNRVIGSGCNLDSARFRYLMGERLGVHALSCHGWVLGEHGDSSVPV 200

Query: 218 WSSISVGGVPILSFLKNQQIAYEKETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLA 277
           WS ++V GV + S         +KE  +++HK+VV  AYEVI LKGYTSWAIG SVA LA
Sbjct: 201 WSGVNVAGVSLKSLNPELGTDADKEQWKEVHKQVVDSAYEVIKLKGYTSWAIGLSVADLA 260

Query: 278 RSLLRDQRRIHPVSVLAKGFYGIDGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDS 337
            S++++ RR+HP+S + KG YGI+  ++FLS+P  LG+ G+  +  V LT EE  RL+ S
Sbjct: 261 ESIMKNLRRVHPISTMIKGLYGIN-EDVFLSVPCILGQNGISDVVKVTLTPEEEARLKKS 320

Query: 338 ANTILQVQTEL 349
           A+T+  +Q EL
Sbjct: 321 ADTLWGIQKEL 330

[Close]

BLAST of Lsi09G004170 vs. ExPASy Swiss-Prot
Match: P04642 (L-lactate dehydrogenase A chain OS=Rattus norvegicus OX=10116 GN=Ldha PE=1 SV=1)

HSP 1 Score: 339.3 bits (869), Expect = 5.0e-92
Identity = 172/311 (55.31%), Postives = 228/311 (73.31%), Query Frame = 0


Query: 38  SKISVIGAGNVGMAIAQTILTQDLVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISAS 97
           +KI+V+G G VGMA A +IL +DL +EL LVD   DKL+GEM+DLQH + FL   KI +S
Sbjct: 21  NKITVVGVGAVGMACAISILMKDLADELALVDVIEDKLKGEMMDLQHGSLFLKTPKIVSS 80

Query: 98  TDYSITAGSDLCIVTAGARQIAGESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPV 157
            DYS+TA S L I+TAGARQ  GESRLNL+QRNV +F+ I+P +V++SP+  LLIVSNPV
Sbjct: 81  KDYSVTANSKLVIITAGARQQEGESRLNLVQRNVNIFKFIIPNVVKYSPQCKLLIVSNPV 140

Query: 158 DVLTYVAWKLSGFPSNRVIGSGTNLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVAL 217
           D+LTYVAWK+SGFP NRVIGSG NLDS+RFR+L+ + L V+      +++GEHGDSSV +
Sbjct: 141 DILTYVAWKISGFPKNRVIGSGCNLDSARFRYLMGERLGVHPLSCHGWVLGEHGDSSVPV 200

Query: 218 WSSISVGGVPILSFLKNQQIAYEKETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLA 277
           WS ++V GV + S         +KE  + +HK+VV  AYEVI LKGYTSWAIG SVA LA
Sbjct: 201 WSGVNVAGVSLKSLNPQLGTDADKEQWKDVHKQVVDSAYEVIKLKGYTSWAIGLSVADLA 260

Query: 278 RSLLRDQRRIHPVSVLAKGFYGIDGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDS 337
            S++++ RR+HP+S + KG YGI   ++FLS+P  LG+ G+  +  V LT +E  RL+ S
Sbjct: 261 ESIMKNLRRVHPISTMIKGLYGIK-EDVFLSVPCILGQNGISDVVKVTLTPDEEARLKKS 320

Query: 338 ANTILQVQTEL 349
           A+T+  +Q EL
Sbjct: 321 ADTLWGIQKEL 330

[Close]

BLAST of Lsi09G004170 vs. ExPASy TrEMBL
Match: A0A1S3ATB9 (L-lactate dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103482619 PE=3 SV=1)

HSP 1 Score: 656.8 bits (1693), Expect = 5.2e-185
Identity = 339/350 (96.86%), Postives = 347/350 (99.14%), Query Frame = 0


Query: 1   MQKSSSTSSLGPGGLDLSQAFFKPIRDASPPSTSKRNSKISVIGAGNVGMAIAQTILTQD 60
           MQKS STSSLGPGGLDLSQAFFKPIRDASPPSTS RN+KISVIGAGNVGMAIAQTILTQD
Sbjct: 1   MQKSPSTSSLGPGGLDLSQAFFKPIRDASPPSTSNRNTKISVIGAGNVGMAIAQTILTQD 60

Query: 61  LVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           LV+ELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYS TAGS+LCIVTAGARQIAG
Sbjct: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSTTAGSNLCIVTAGARQIAG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 240
           NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 240

Query: 241 KETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI 300
           KETLEKIHKEV+GGAYEVI+LKGYTSWAIGYSVASLARS+LRDQRRIHPVSVLAKGFYGI
Sbjct: 241 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGI 300

Query: 301 DGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDSANTILQVQTELGI 351
           DGG+LFLSLPAQLGRGGVLGITNVHLTAEES+RLRDSANTILQVQTELGI
Sbjct: 301 DGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI 350

[Close]

BLAST of Lsi09G004170 vs. ExPASy TrEMBL
Match: A0A5A7T9X6 (L-lactate dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold558G00390 PE=3 SV=1)

HSP 1 Score: 654.4 bits (1687), Expect = 2.6e-184
Identity = 338/350 (96.57%), Postives = 346/350 (98.86%), Query Frame = 0


Query: 1   MQKSSSTSSLGPGGLDLSQAFFKPIRDASPPSTSKRNSKISVIGAGNVGMAIAQTILTQD 60
           MQKS S SSLGPGGLDLSQAFFKPIRDASPPS S RN+KISVIGAGNVGMAIAQTILTQD
Sbjct: 1   MQKSPSASSLGPGGLDLSQAFFKPIRDASPPSISNRNTKISVIGAGNVGMAIAQTILTQD 60

Query: 61  LVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           LV+ELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGS+LCIVTAGARQIAG
Sbjct: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSNLCIVTAGARQIAG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 240
           NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 240

Query: 241 KETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI 300
           KETLEKIHKEV+GGAYEVI+LKGYTSWAIGYSVASLARS+LRDQRRIHPVSVLAKGFYGI
Sbjct: 241 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGI 300

Query: 301 DGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDSANTILQVQTELGI 351
           DGG+LFLSLPAQLGRGGVLGITNVHLTAEES+RLRDSANTILQVQTELGI
Sbjct: 301 DGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI 350

[Close]

BLAST of Lsi09G004170 vs. ExPASy TrEMBL
Match: A0A6J1E3A1 (L-lactate dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111430410 PE=3 SV=1)

HSP 1 Score: 641.7 bits (1654), Expect = 1.7e-180
Identity = 330/350 (94.29%), Postives = 342/350 (97.71%), Query Frame = 0


Query: 1   MQKSSSTSSLGPGGLDLSQAFFKPIRDASPPSTSKRNSKISVIGAGNVGMAIAQTILTQD 60
           M+KSSS SSLGPGGLD+SQAFFKP+ DASPPSTSKRN+KISVIGAGNVGMAIAQTILTQD
Sbjct: 1   MEKSSSASSLGPGGLDISQAFFKPVSDASPPSTSKRNTKISVIGAGNVGMAIAQTILTQD 60

Query: 61  LVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           L +ELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYS+TA SDLCIVTAGARQIAG
Sbjct: 61  LADELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSVTAESDLCIVTAGARQIAG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALFQKIVPPLVQ+SPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVALFQKIVPPLVQYSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 240
           NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFL+NQQI YE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLQNQQITYE 240

Query: 241 KETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI 300
           KETLE IHKEV+GGAYEVI+LKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI
Sbjct: 241 KETLESIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI 300

Query: 301 DGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDSANTILQVQTELGI 351
           +GGELFLSLPAQLGRGGVLGITNVHLT EES RLRDSANTILQVQT+LGI
Sbjct: 301 NGGELFLSLPAQLGRGGVLGITNVHLTDEESGRLRDSANTILQVQTQLGI 350

[Close]

BLAST of Lsi09G004170 vs. ExPASy TrEMBL
Match: A0A6J1HYL4 (L-lactate dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111468835 PE=3 SV=1)

HSP 1 Score: 639.0 bits (1647), Expect = 1.1e-179
Identity = 328/350 (93.71%), Postives = 342/350 (97.71%), Query Frame = 0


Query: 1   MQKSSSTSSLGPGGLDLSQAFFKPIRDASPPSTSKRNSKISVIGAGNVGMAIAQTILTQD 60
           M+KSSS SSLGPGGL++SQAFFKP+ DASPPSTSKRN+KISVIGAGNVGMAIAQTILTQD
Sbjct: 1   MEKSSSASSLGPGGLNISQAFFKPVSDASPPSTSKRNTKISVIGAGNVGMAIAQTILTQD 60

Query: 61  LVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           L +ELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYS+TA SDLCIVTAGARQIAG
Sbjct: 61  LADELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSVTAESDLCIVTAGARQIAG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALFQKIVPPLVQ+SPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVALFQKIVPPLVQYSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 240
           NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFL+NQQI YE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLQNQQITYE 240

Query: 241 KETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI 300
           KETLE IHKEV+GGAYEVI+LKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI
Sbjct: 241 KETLESIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI 300

Query: 301 DGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDSANTILQVQTELGI 351
           +GGE+FLSLPAQLGRGGVLGITNVHLT EES RLRDSANTILQVQT+LGI
Sbjct: 301 NGGEMFLSLPAQLGRGGVLGITNVHLTDEESGRLRDSANTILQVQTQLGI 350

[Close]

BLAST of Lsi09G004170 vs. ExPASy TrEMBL
Match: A0A6J1DPR5 (L-lactate dehydrogenase OS=Momordica charantia OX=3673 GN=LOC111021951 PE=3 SV=1)

HSP 1 Score: 637.1 bits (1642), Expect = 4.3e-179
Identity = 327/350 (93.43%), Postives = 342/350 (97.71%), Query Frame = 0


Query: 1   MQKSSSTSSLGPGGLDLSQAFFKPIRDASPPSTSKRNSKISVIGAGNVGMAIAQTILTQD 60
           MQKSSS S LGPGGLD+SQAFFKPIRDA+PPS  KR++KISVIGAGNVGMAIAQTILTQD
Sbjct: 1   MQKSSSESLLGPGGLDISQAFFKPIRDAAPPSALKRHTKISVIGAGNVGMAIAQTILTQD 60

Query: 61  LVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           LVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKI+ASTDYS+TAGSDLCIVTAGARQIAG
Sbjct: 61  LVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKINASTDYSVTAGSDLCIVTAGARQIAG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALFQKIV PLVQ+SP+TILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVALFQKIVXPLVQYSPDTILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 240
           NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 240

Query: 241 KETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI 300
           KETLE IHKEV+GGAYEVISLKGYTSWAIGYSVASLARSL+RDQRRIHPVSVLAKGFYGI
Sbjct: 241 KETLENIHKEVIGGAYEVISLKGYTSWAIGYSVASLARSLIRDQRRIHPVSVLAKGFYGI 300

Query: 301 DGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDSANTILQVQTELGI 351
           DGGELFLSLPAQLGRGGVLGITNVHLT +E RRLR+SANTIL+VQ++LGI
Sbjct: 301 DGGELFLSLPAQLGRGGVLGITNVHLTEDECRRLRESANTILEVQSQLGI 350

[Close]

BLAST of Lsi09G004170 vs. NCBI nr
Match: XP_038906687.1 (L-lactate dehydrogenase A [Benincasa hispida])

HSP 1 Score: 661.4 bits (1705), Expect = 4.4e-186
Identity = 342/350 (97.71%), Postives = 347/350 (99.14%), Query Frame = 0


Query: 1   MQKSSSTSSLGPGGLDLSQAFFKPIRDASPPSTSKRNSKISVIGAGNVGMAIAQTILTQD 60
           MQKSSSTSSLGPGGLDLSQAFFKPIRDASPPSTSKRN+KISVIG GNVGMAIAQTILTQD
Sbjct: 1   MQKSSSTSSLGPGGLDLSQAFFKPIRDASPPSTSKRNTKISVIGTGNVGMAIAQTILTQD 60

Query: 61  LVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           LV+ELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCI+TAGARQIAG
Sbjct: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIITAGARQIAG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 240
           NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 240

Query: 241 KETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI 300
           KETLEKIHKEV+GGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKG YGI
Sbjct: 241 KETLEKIHKEVIGGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGLYGI 300

Query: 301 DGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDSANTILQVQTELGI 351
           DGG+LFLSLPAQLGRGGVLGITNVHLT EESRRLRDSANTILQVQTELGI
Sbjct: 301 DGGDLFLSLPAQLGRGGVLGITNVHLTEEESRRLRDSANTILQVQTELGI 350

[Close]

BLAST of Lsi09G004170 vs. NCBI nr
Match: XP_004151663.3 (L-lactate dehydrogenase A-like [Cucumis sativus])

HSP 1 Score: 657.1 bits (1694), Expect = 8.3e-185
Identity = 338/350 (96.57%), Postives = 347/350 (99.14%), Query Frame = 0


Query: 1   MQKSSSTSSLGPGGLDLSQAFFKPIRDASPPSTSKRNSKISVIGAGNVGMAIAQTILTQD 60
           MQKSSS SSLGPGGLDLSQAFFKPIRDASPPST+ RN+KISVIGAGNVGMAIAQTILTQD
Sbjct: 1   MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQD 60

Query: 61  LVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           LV+ELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG
Sbjct: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTY+AWKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 240
           NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQI YE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYE 240

Query: 241 KETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI 300
           KETLEKIHKEV+GGAYEVI+LKGYTSWAIGYSVASLARS+LRDQRRIHPVSVLAKGFYGI
Sbjct: 241 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGI 300

Query: 301 DGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDSANTILQVQTELGI 351
           DGG+LFLSLPAQLGRGGVLGITNVHLTAEES+RLRDSANTILQVQTELGI
Sbjct: 301 DGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI 350

[Close]

BLAST of Lsi09G004170 vs. NCBI nr
Match: XP_008437100.2 (PREDICTED: L-lactate dehydrogenase A [Cucumis melo])

HSP 1 Score: 656.8 bits (1693), Expect = 1.1e-184
Identity = 339/350 (96.86%), Postives = 347/350 (99.14%), Query Frame = 0


Query: 1   MQKSSSTSSLGPGGLDLSQAFFKPIRDASPPSTSKRNSKISVIGAGNVGMAIAQTILTQD 60
           MQKS STSSLGPGGLDLSQAFFKPIRDASPPSTS RN+KISVIGAGNVGMAIAQTILTQD
Sbjct: 1   MQKSPSTSSLGPGGLDLSQAFFKPIRDASPPSTSNRNTKISVIGAGNVGMAIAQTILTQD 60

Query: 61  LVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           LV+ELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYS TAGS+LCIVTAGARQIAG
Sbjct: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSTTAGSNLCIVTAGARQIAG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 240
           NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 240

Query: 241 KETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI 300
           KETLEKIHKEV+GGAYEVI+LKGYTSWAIGYSVASLARS+LRDQRRIHPVSVLAKGFYGI
Sbjct: 241 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGI 300

Query: 301 DGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDSANTILQVQTELGI 351
           DGG+LFLSLPAQLGRGGVLGITNVHLTAEES+RLRDSANTILQVQTELGI
Sbjct: 301 DGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI 350

[Close]

BLAST of Lsi09G004170 vs. NCBI nr
Match: KAA0039708.1 (L-lactate dehydrogenase A [Cucumis melo var. makuwa])

HSP 1 Score: 654.4 bits (1687), Expect = 5.4e-184
Identity = 338/350 (96.57%), Postives = 346/350 (98.86%), Query Frame = 0


Query: 1   MQKSSSTSSLGPGGLDLSQAFFKPIRDASPPSTSKRNSKISVIGAGNVGMAIAQTILTQD 60
           MQKS S SSLGPGGLDLSQAFFKPIRDASPPS S RN+KISVIGAGNVGMAIAQTILTQD
Sbjct: 1   MQKSPSASSLGPGGLDLSQAFFKPIRDASPPSISNRNTKISVIGAGNVGMAIAQTILTQD 60

Query: 61  LVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           LV+ELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGS+LCIVTAGARQIAG
Sbjct: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSNLCIVTAGARQIAG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 240
           NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 240

Query: 241 KETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI 300
           KETLEKIHKEV+GGAYEVI+LKGYTSWAIGYSVASLARS+LRDQRRIHPVSVLAKGFYGI
Sbjct: 241 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGI 300

Query: 301 DGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDSANTILQVQTELGI 351
           DGG+LFLSLPAQLGRGGVLGITNVHLTAEES+RLRDSANTILQVQTELGI
Sbjct: 301 DGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI 350

[Close]

BLAST of Lsi09G004170 vs. NCBI nr
Match: KAE8647895.1 (hypothetical protein Csa_000230 [Cucumis sativus])

HSP 1 Score: 649.4 bits (1674), Expect = 1.7e-182
Identity = 333/350 (95.14%), Postives = 345/350 (98.57%), Query Frame = 0


Query: 1   MQKSSSTSSLGPGGLDLSQAFFKPIRDASPPSTSKRNSKISVIGAGNVGMAIAQTILTQD 60
           MQKSSS SSLGPGGLDLSQAFFKPIRDASPPST+ RN+KISVIGAGNVGMAIAQTILTQD
Sbjct: 1   MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQD 60

Query: 61  LVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           LV+ELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG
Sbjct: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTY+AWKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 240
           NLDSSRFRFLIADHLDVNAQDVQA+IVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAFIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 240

Query: 241 KETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI 300
           KETLEKIHKEV+GGAYEVI+LKGYTSWAIGYSVASLARS+LRDQRRIHPVSV+AKGFYGI
Sbjct: 241 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVIAKGFYGI 300

Query: 301 DGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDSANTILQVQTELGI 351
           +GG+LFLSLPAQLGRGGVLGITNVHLT EES+RLRDSANTIL VQ ELGI
Sbjct: 301 EGGDLFLSLPAQLGRGGVLGITNVHLTTEESKRLRDSANTILHVQAELGI 350

[Close]

BLAST of Lsi09G004170 vs. TAIR 10
Match: AT4G17260.1 (Lactate/malate dehydrogenase family protein )

HSP 1 Score: 546.6 bits (1407), Expect = 1.5e-155
Identity = 271/353 (76.77%), Postives = 317/353 (89.80%), Query Frame = 0


Query: 1   MQKSSSTSS---LGPGGLDLSQAFFKPIRDASPPSTSKRNSKISVIGAGNVGMAIAQTIL 60
           M+K++STSS   LGP GLDL+ AFFKPI ++ P   S R +K+SV+G GNVGMAIAQTIL
Sbjct: 1   MEKNASTSSLKDLGPSGLDLTSAFFKPIHNSDPSLPSNRRTKVSVVGVGNVGMAIAQTIL 60

Query: 61  TQDLVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQ 120
           TQDL +E+ LVDAKPDKLRGEMLDLQHAAAFLPRTKI+AS DY +TAGSDLCIVTAGARQ
Sbjct: 61  TQDLADEIALVDAKPDKLRGEMLDLQHAAAFLPRTKITASVDYEVTAGSDLCIVTAGARQ 120

Query: 121 IAGESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIG 180
             GESRLNLLQRNVALF+ I+PPL + SP++IL+IVSNPVDVLTYVAWKLSGFP NRV+G
Sbjct: 121 NPGESRLNLLQRNVALFRHIIPPLAKASPDSILIIVSNPVDVLTYVAWKLSGFPVNRVLG 180

Query: 181 SGTNLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQI 240
           SGTNLDSSRFRFLIADHLDVNAQDVQA+IVGEHGDSSVALWSSISVGG+P+LSFL+  QI
Sbjct: 181 SGTNLDSSRFRFLIADHLDVNAQDVQAFIVGEHGDSSVALWSSISVGGIPVLSFLEKNQI 240

Query: 241 AYEKETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGF 300
           AYEK+TLE IH+ VVG AYEVI LKGYTSWAIGYSVA+LAR++LRDQR+IHPV+VLA+GF
Sbjct: 241 AYEKQTLEDIHQAVVGSAYEVIGLKGYTSWAIGYSVANLARTILRDQRKIHPVTVLARGF 300

Query: 301 YGIDGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDSANTILQVQTELGI 351
           YG+DGG++FLSLPA LGR GV+ +TNVH+T EE+ +L+ SA TIL++Q++LG+
Sbjct: 301 YGVDGGDVFLSLPALLGRNGVVAVTNVHMTDEEAEKLQKSAKTILEMQSQLGL 353

The following BLAST results are available for this feature:

BLAST of Lsi09G004170 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P22988	8.7e-129	69.83	L-lactate dehydrogenase A OS=Hordeum vulgare OX=4513 PE=1 SV=1	[more]
P22989	1.2e-125	67.84	L-lactate dehydrogenase B (Fragment) OS=Hordeum vulgare OX=4513 PE=1 SV=1	[more]
P29038	3.0e-121	66.48	L-lactate dehydrogenase OS=Zea mays OX=4577 PE=2 SV=1	[more]
P06151	5.9e-93	55.63	L-lactate dehydrogenase A chain OS=Mus musculus OX=10090 GN=Ldha PE=1 SV=3	[more]
P04642	5.0e-92	55.31	L-lactate dehydrogenase A chain OS=Rattus norvegicus OX=10116 GN=Ldha PE=1 SV=1	[more]

BLAST of Lsi09G004170 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3ATB9	5.2e-185	96.86	L-lactate dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103482619 PE=3 SV=1	[more]
A0A5A7T9X6	2.6e-184	96.57	L-lactate dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold...	[more]
A0A6J1E3A1	1.7e-180	94.29	L-lactate dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111430410 PE=3 SV=1	[more]
A0A6J1HYL4	1.1e-179	93.71	L-lactate dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111468835 PE=3 SV=1	[more]
A0A6J1DPR5	4.3e-179	93.43	L-lactate dehydrogenase OS=Momordica charantia OX=3673 GN=LOC111021951 PE=3 SV=1	[more]

BLAST of Lsi09G004170 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038906687.1	4.4e-186	97.71	L-lactate dehydrogenase A [Benincasa hispida]	[more]
XP_004151663.3	8.3e-185	96.57	L-lactate dehydrogenase A-like [Cucumis sativus]	[more]
XP_008437100.2	1.1e-184	96.86	PREDICTED: L-lactate dehydrogenase A [Cucumis melo]	[more]
KAA0039708.1	5.4e-184	96.57	L-lactate dehydrogenase A [Cucumis melo var. makuwa]	[more]
KAE8647895.1	1.7e-182	95.14	hypothetical protein Csa_000230 [Cucumis sativus]	[more]

BLAST of Lsi09G004170 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G17260.1	1.5e-155	76.77	Lactate/malate dehydrogenase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001557	L-lactate/malate dehydrogenase	PRINTS	PR00086	LLDHDRGNASE	coord: 39..63 score: 45.78 coord: 64..88 score: 51.92 coord: 205..218 score: 67.82 coord: 175..193 score: 68.42 coord: 151..171 score: 71.81
IPR001557	L-lactate/malate dehydrogenase	PIRSF	PIRSF000102	Lac_mal_DH	coord: 34..350 e-value: 1.4E-102 score: 340.3
IPR022383	Lactate/malate dehydrogenase, C-terminal	PFAM	PF02866	Ldh_1_C	coord: 180..342 e-value: 1.4E-23 score: 83.8
IPR015955	Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal	GENE3D	3.90.110.10		coord: 180..348 e-value: 3.2E-59 score: 201.2
IPR015955	Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal	SUPERFAMILY	56327	LDH C-terminal domain-like	coord: 178..346
None	No IPR available	GENE3D	3.40.50.720		coord: 34..179 e-value: 1.2E-57 score: 196.0
None	No IPR available	PANTHER	PTHR43128	L-2-HYDROXYCARBOXYLATE DEHYDROGENASE (NAD(P)(+))	coord: 1..350
None	No IPR available	PANTHER	PTHR43128:SF23	L-LACTATE DEHYDROGENASE	coord: 1..350
None	No IPR available	CDD	cd05293	LDH_1	coord: 35..347 e-value: 1.67502E-175 score: 487.497
IPR011304	L-lactate dehydrogenase	TIGRFAM	TIGR01771	TIGR01771	coord: 42..341 e-value: 5.2E-115 score: 381.8
IPR011304	L-lactate dehydrogenase	HAMAP	MF_00488	Lactate_dehydrog	coord: 37..349 score: 37.019402
IPR001236	Lactate/malate dehydrogenase, N-terminal	PFAM	PF00056	Ldh_1_N	coord: 39..177 e-value: 4.6E-43 score: 146.7
IPR018177	L-lactate dehydrogenase, active site	PROSITE	PS00064	L_LDH	coord: 207..213
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 24..177

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi09G004170.1	Lsi09G004170.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0019752	carboxylic acid metabolic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0004459	L-lactate dehydrogenase activity
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0016616	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor