Lsi09G003420 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi09G003420
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Subtilisin-like protease SBT1.7
Location	chr09: 3543450 .. 3544001 (+)
RNA-Seq Expression	Lsi09G003420
Synteny	Lsi09G003420

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACTACTGCAAACATAAAAACTCCTCAAGGAAAACCAATTCTGGATCAAGATTTGAAACCGGCTAACTTTTTTGCAATTGGTGCTGGACATGTAAACCCATCAAAGGCAGCTGACCCAGGATTGGTTTATGACATTCAATCTGATGATTACATTCCTTATCTTTGTGGTTTGTACAAAGATGAAGAAGTTTCAATTATTGTTCATAAAACAGTAATCTGCGGATTAGTACCAAGGATTCGAGAAGGGGATTTGAATTATCCTTCGTTTAGTGTCGAGTTAGGAGGCTTACAAACGTTCAAAAGAACAGTGACAAATGTGGGTGAAGCAAACTCGGTTTATTCTGTCATGGTTGAAGCACCGCTGGGAGTTTCTATGACGGTCTTACCAAGAAAATTGGTGTTCTCGAGGGTGAACCAAAAGTTGACCTTTTCGGTGACTTTTAGTCGAATTAGCTCTGTTAAAATAATAGGTGAATTTGGTCAAGGATATCTCAAATGGGTTTCTCAAAAACATGTTGTGAGGAGTCCCATTTCTGTGAAGTTTAATTAA

mRNA sequence

ATGACTACTGCAAACATAAAAACTCCTCAAGGAAAACCAATTCTGGATCAAGATTTGAAACCGGCTAACTTTTTTGCAATTGGTGCTGGACATGTAAACCCATCAAAGGCAGCTGACCCAGGATTGGTTTATGACATTCAATCTGATGATTACATTCCTTATCTTTGTGGTTTGTACAAAGATGAAGAAGTTTCAATTATTGTTCATAAAACAGTAATCTGCGGATTAGTACCAAGGATTCGAGAAGGGGATTTGAATTATCCTTCGTTTAGTGTCGAGTTAGGAGGCTTACAAACGTTCAAAAGAACAGTGACAAATGTGGGTGAAGCAAACTCGGTTTATTCTGTCATGGTTGAAGCACCGCTGGGAGTTTCTATGACGGTCTTACCAAGAAAATTGGTGTTCTCGAGGGTGAACCAAAAGTTGACCTTTTCGGTGACTTTTAGTCGAATTAGCTCTGTTAAAATAATAGGTGAATTTGGTCAAGGATATCTCAAATGGGTTTCTCAAAAACATGTTGTGAGGAGTCCCATTTCTGTGAAGTTTAATTAA

Coding sequence (CDS)

ATGACTACTGCAAACATAAAAACTCCTCAAGGAAAACCAATTCTGGATCAAGATTTGAAACCGGCTAACTTTTTTGCAATTGGTGCTGGACATGTAAACCCATCAAAGGCAGCTGACCCAGGATTGGTTTATGACATTCAATCTGATGATTACATTCCTTATCTTTGTGGTTTGTACAAAGATGAAGAAGTTTCAATTATTGTTCATAAAACAGTAATCTGCGGATTAGTACCAAGGATTCGAGAAGGGGATTTGAATTATCCTTCGTTTAGTGTCGAGTTAGGAGGCTTACAAACGTTCAAAAGAACAGTGACAAATGTGGGTGAAGCAAACTCGGTTTATTCTGTCATGGTTGAAGCACCGCTGGGAGTTTCTATGACGGTCTTACCAAGAAAATTGGTGTTCTCGAGGGTGAACCAAAAGTTGACCTTTTCGGTGACTTTTAGTCGAATTAGCTCTGTTAAAATAATAGGTGAATTTGGTCAAGGATATCTCAAATGGGTTTCTCAAAAACATGTTGTGAGGAGTCCCATTTCTGTGAAGTTTAATTAA

Protein sequence

MTTANIKTPQGKPILDQDLKPANFFAIGAGHVNPSKAADPGLVYDIQSDDYIPYLCGLYKDEEVSIIVHKTVICGLVPRIREGDLNYPSFSVELGGLQTFKRTVTNVGEANSVYSVMVEAPLGVSMTVLPRKLVFSRVNQKLTFSVTFSRISSVKIIGEFGQGYLKWVSQKHVVRSPISVKFN

Homology

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BLAST of Lsi09G003420 vs. ExPASy Swiss-Prot
Match: A9QY38 (Subtilisin-like protease 4 OS=Lotus japonicus OX=34305 GN=SBTM4 PE=2 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 2.0e-55
Identity = 112/183 (61.20%), Postives = 132/183 (72.13%), Query Frame = 0


Query: 1   MTTANIKTPQGKPILDQDLKPANFFAIGAGHVNPSKAADPGLVYDIQSDDYIPYLCGL-Y 60
           MT+A+      K I+D+ L+P + FA G+GHVNPS+A DPGLVYDIQ DDYIPYLCGL Y
Sbjct: 574 MTSADTINLGNKLIVDETLQPTDLFATGSGHVNPSRANDPGLVYDIQPDDYIPYLCGLGY 633

Query: 61  KDEEVSIIVHKTVICGLVPRIREGDLNYPSFSVELGGLQTFKRTVTNVGEANSVYSVMVE 120
            + EV II H+ + C     I EG+LNYPSFSVELG  +TF RTVTNVGEA+S Y ++V 
Sbjct: 634 SETEVGIIAHRKIKCS--ASIPEGELNYPSFSVELGSSKTFTRTVTNVGEAHSSYDLIVA 693

Query: 121 APLGVSMTVLPRKLVFSRVNQKLTFSVTFSRISSVKIIGEFGQGYLKWVSQKHVVRSPIS 180
           AP GV + V P KL FS VNQK T+SVTFSR        E+ QG+LKWVS KH VRSPIS
Sbjct: 694 APQGVDVKVQPYKLNFSEVNQKETYSVTFSRTGLGNKTQEYAQGFLKWVSTKHTVRSPIS 753

Query: 181 VKF 183
           VKF
Sbjct: 754 VKF 754

[Close]

BLAST of Lsi09G003420 vs. ExPASy Swiss-Prot
Match: A9QY40 (Subtilisin-like protease 1 OS=Lotus japonicus OX=34305 GN=SBTM1 PE=2 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 2.7e-49
Identity = 101/185 (54.59%), Postives = 131/185 (70.81%), Query Frame = 0


Query: 1   MTTANIKTPQGKPILDQDLKPANFFAIGAGHVNPSKAADPGLVYDIQSDDYIPYLCGL-Y 60
           MTTAN    +G PILDQ L+PA+ FA GAGHVNP +A DPGLVYDIQ +DY+PYLCGL Y
Sbjct: 565 MTTANTLNLRGLPILDQRLQPADIFATGAGHVNPVRANDPGLVYDIQPEDYVPYLCGLGY 624

Query: 61  KDEEVSIIVHKTVICGLVPRIREGDLNYPSFSVELGG-LQTFKRTVTNVGEANSVYSVMV 120
            D EV+IIV ++V C  V  I + +LNYPSFS+ LG   Q + RT+TNVG ANS Y+V +
Sbjct: 625 SDREVTIIVQRSVRCFNVKSIAQAELNYPSFSILLGSDSQFYTRTLTNVGPANSTYTVKI 684

Query: 121 EAPLGVSMTVLPRKLVFSRVNQKLTFSVTF-SRISSVKIIGEFGQGYLKWVSQKHVVRSP 180
           + PL + ++V P ++ F++VNQK+ + V F  +I   +    F QG + WVS KHVVR+P
Sbjct: 685 DVPLAMGISVSPSQITFTQVNQKVAYFVDFIPQIKENRGNHTFAQGAITWVSDKHVVRTP 744

Query: 181 ISVKF 183
           ISV F
Sbjct: 745 ISVIF 749

[Close]

BLAST of Lsi09G003420 vs. ExPASy Swiss-Prot
Match: A9QY39 (Subtilisin-like protease 3 OS=Lotus japonicus OX=34305 GN=SBTM3 PE=2 SV=1)

HSP 1 Score: 194.1 bits (492), Expect = 1.4e-48
Identity = 103/184 (55.98%), Postives = 124/184 (67.39%), Query Frame = 0


Query: 1   MTTANIKTPQGKPILDQDLKPANFFAIGAGHVNPSKAADPGLVYDIQSDDYIPYLCGL-Y 60
           MTTA+     G PILDQ L PA+ FA GAGHVNP KA DPGLVYDI+ +DYIPYLCGL Y
Sbjct: 566 MTTASQVNLGGTPILDQRLVPADVFATGAGHVNPVKANDPGLVYDIEPNDYIPYLCGLNY 625

Query: 61  KDEEVSIIVHKTVICGLVPRIREGDLNYPSFSVELGG-LQTFKRTVTNVGEANSVYSVMV 120
            D EV +I+ + V C  V  I E +LNYPSFS+ LG   Q + RTV NVG ANS Y+  +
Sbjct: 626 TDREVGVILQQRVRCSEVNHIAEAELNYPSFSILLGNTTQLYTRTVANVGPANSTYTAEI 685

Query: 121 EAPLGVSMTVLPRKLVFSRVNQKLTFSVTFSRISSVKIIGEFGQGYLKWVSQKHVVRSPI 180
             P+GV M++ P +L F+ V QKLT+SV+F   S  +    F QG LKWVS K+ VRSPI
Sbjct: 686 GVPVGVGMSLSPAQLTFTEVGQKLTYSVSFIPFSEDRDNHTFAQGSLKWVSGKYSVRSPI 745

Query: 181 SVKF 183
           S  F
Sbjct: 746 SFIF 749

[Close]

BLAST of Lsi09G003420 vs. ExPASy Swiss-Prot
Match: G7KEU7 (Subtilisin-like protease OS=Medicago truncatula OX=3880 GN=SBT PE=2 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 3.0e-48
Identity = 103/185 (55.68%), Postives = 125/185 (67.57%), Query Frame = 0


Query: 1   MTTANIKTPQGKPILDQDLKPANFFAIGAGHVNPSKAADPGLVYDIQSDDYIPYLCGL-Y 60
           MTTAN     G PILDQ L PA+ FA GAGHVNP KA DPGLVYDI+ +DY+PYLCGL Y
Sbjct: 563 MTTANTLNLGGIPILDQRLFPADIFATGAGHVNPVKANDPGLVYDIEPEDYVPYLCGLGY 622

Query: 61  KDEEVSIIVHKTVICGLVPRIREGDLNYPSFSVELGG-LQTFKRTVTNVGEANSVYSVMV 120
            D+E+ +IV   V C  V  I E  LNYPSFS+ LG   Q + RT+TNVG ANS Y V +
Sbjct: 623 SDKEIEVIVQWKVKCSNVKSIPEAQLNYPSFSILLGSDSQYYTRTLTNVGFANSTYKVEL 682

Query: 121 EAPLGVSMTVLPRKLVFSRVNQKLTFSVTF-SRISSVKIIGEFGQGYLKWVSQKHVVRSP 180
           E PL + M+V P ++ F+ VN+K++FSV F  +I   +    FGQG L WVS +H VR P
Sbjct: 683 EVPLALGMSVNPSEITFTEVNEKVSFSVEFIPQIKENRRNHTFGQGSLTWVSDRHAVRIP 742

Query: 181 ISVKF 183
           ISV F
Sbjct: 743 ISVIF 747

[Close]

BLAST of Lsi09G003420 vs. ExPASy Swiss-Prot
Match: A0A0M3R8G2 (Subtilisin-like protease OS=Petunia hybrida OX=4102 GN=SBT PE=2 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 1.3e-43
Identity = 93/183 (50.82%), Postives = 129/183 (70.49%), Query Frame = 0


Query: 1   MTTANIKTPQGKPILDQDLKPANFFAIGAGHVNPSKAADPGLVYDIQSDDYIPYLCGL-Y 60
           MT+A+    +G+PILD+   PA+ FA GAGHVNPSKA+DPGL+YDIQ +DYI YLCGL Y
Sbjct: 554 MTSADQSNLEGQPILDERNLPADIFATGAGHVNPSKASDPGLIYDIQLEDYIQYLCGLGY 613

Query: 61  KDEEVSIIVHKTVICGLVPRIREGDLNYPSFSVELG-GLQTFKRTVTNVGEANSVYSVMV 120
           +++++ +IV +TV C     I E +LNYPSFS+ LG   Q + RTVTNVG+A+S Y+V +
Sbjct: 614 REKDIGLIVQETVKC--ESSISEAELNYPSFSIILGPKTQNYTRTVTNVGDASSTYTVNI 673

Query: 121 EAPLGVSMTVLPRKLVFSRVNQKLTFSVTFSRISSVKIIGEFGQGYLKWVSQKHVVRSPI 180
               GV + V P  LVF+++ Q+ T++V+F++  S      F QG + W S ++VVRSPI
Sbjct: 674 AKTQGVDIVVEPATLVFTKMYQQATYTVSFTQ--SGDFTDRFVQGAISWSSNEYVVRSPI 732

Query: 181 SVK 182
           SVK
Sbjct: 734 SVK 732

[Close]

BLAST of Lsi09G003420 vs. ExPASy TrEMBL
Match: A0A5A7TMM4 (Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G003280 PE=3 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 3.8e-86
Identity = 158/184 (85.87%), Postives = 174/184 (94.57%), Query Frame = 0


Query: 1   MTTANIKTPQGKPILDQDLKPANFFAIGAGHVNPSKAADPGLVYDIQSDDYIPYLCGLYK 60
           MTTANIKTPQG+PI+DQDL+PANFFA+GAGHVNPSKAADPGLVYDIQ DDYIPYLCGLYK
Sbjct: 571 MTTANIKTPQGEPIVDQDLQPANFFAMGAGHVNPSKAADPGLVYDIQPDDYIPYLCGLYK 630

Query: 61  DEEVSIIVHKTVICGLVPRIREGDLNYPSFSVELGGLQTFKRTVTNVGEANSVYSVMVEA 120
           DEEVSIIVH+TVICGLVP IREGDLNYPSFSV LGGLQTFKRTVTNVGEANSVY+ +VEA
Sbjct: 631 DEEVSIIVHRTVICGLVPSIREGDLNYPSFSVALGGLQTFKRTVTNVGEANSVYTAIVEA 690

Query: 121 PLGVSMTVLPRKLVFSRVNQKLTFSVTFSRISSVKIIGEFGQGYLKWV--SQKHVVRSPI 180
           PLGVSMTV+PRKL FSRVNQ +TF+VTF+RI  VKI+GEFG+GYLKWV  S+K+VVRSP+
Sbjct: 691 PLGVSMTVMPRKLFFSRVNQTMTFTVTFNRIRWVKIVGEFGEGYLKWVSKSKKYVVRSPV 750

Query: 181 SVKF 183
           SVKF
Sbjct: 751 SVKF 754

[Close]

BLAST of Lsi09G003420 vs. ExPASy TrEMBL
Match: A0A1S3AUP1 (subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103483066 PE=3 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 9.4e-85
Identity = 157/184 (85.33%), Postives = 172/184 (93.48%), Query Frame = 0


Query: 1   MTTANIKTPQGKPILDQDLKPANFFAIGAGHVNPSKAADPGLVYDIQSDDYIPYLCGLYK 60
           MTTANIKTPQG+PI DQDL+PANFFA+GAGHVNPSKAADPGLVYDIQ DDYIPYLCGLYK
Sbjct: 580 MTTANIKTPQGEPITDQDLQPANFFAMGAGHVNPSKAADPGLVYDIQPDDYIPYLCGLYK 639

Query: 61  DEEVSIIVHKTVICGLVPRIREGDLNYPSFSVELGGLQTFKRTVTNVGEANSVYSVMVEA 120
           DEEVSIIVH+TVICGLV  IREGDLNYPSFSV LGGLQTFKRTVTNVGEANSVY+ +VEA
Sbjct: 640 DEEVSIIVHRTVICGLVLSIREGDLNYPSFSVALGGLQTFKRTVTNVGEANSVYTAIVEA 699

Query: 121 PLGVSMTVLPRKLVFSRVNQKLTFSVTFSRISSVKIIGEFGQGYLKWV--SQKHVVRSPI 180
           PLGVSMTV+PRKL FSRVNQ +TF+VTF+RI  VKI+GEFG+GYLKWV  S+K+VVRSP+
Sbjct: 700 PLGVSMTVMPRKLFFSRVNQTMTFTVTFNRIRWVKIVGEFGEGYLKWVSKSKKYVVRSPV 759

Query: 181 SVKF 183
           SVKF
Sbjct: 760 SVKF 763

[Close]

BLAST of Lsi09G003420 vs. ExPASy TrEMBL
Match: A0A1S3AT16 (subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103482683 PE=3 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 3.6e-68
Identity = 130/182 (71.43%), Postives = 152/182 (83.52%), Query Frame = 0


Query: 1   MTTANIKTPQGKPILDQDLKPANFFAIGAGHVNPSKAADPGLVYDIQSDDYIPYLCGLYK 60
           MT+A+++ PQGKPI+DQDLKPANFFA+G+GHVNPSKAA+PGLVYDIQ DDYIPYLC LY 
Sbjct: 571 MTSADVRNPQGKPIMDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDDYIPYLCHLYT 630

Query: 61  DEEVSIIVHKTVICGLVPRIREGDLNYPSFSVELG-GLQTFKRTVTNVGEANSVYSVMVE 120
           D +VSIIV K V C  VPRIREGDLNYPSF+V LG G QTF RTVTNVG+ANSVY  +VE
Sbjct: 631 DAQVSIIVRKQVTCSTVPRIREGDLNYPSFAVSLGAGSQTFNRTVTNVGDANSVYYPIVE 690

Query: 121 APLGVSMTVLPRKLVFSRVNQKLTFSVTFSRISSVKIIGEFGQGYLKWVSQKHVVRSPIS 180
           AP GVS+ V P  L FS++N+K+T+SVTFSRI  V+   EF +GYL WVS+K +VRSPIS
Sbjct: 691 APAGVSVKVTPSNLKFSKLNEKVTYSVTFSRIDFVRTTSEFSEGYLIWVSKKLMVRSPIS 750

Query: 181 VK 182
           VK
Sbjct: 751 VK 752

[Close]

BLAST of Lsi09G003420 vs. ExPASy TrEMBL
Match: A0A5A7THE4 (Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G003290 PE=3 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 1.8e-67
Identity = 129/182 (70.88%), Postives = 151/182 (82.97%), Query Frame = 0


Query: 1   MTTANIKTPQGKPILDQDLKPANFFAIGAGHVNPSKAADPGLVYDIQSDDYIPYLCGLYK 60
           MT+A+++ PQGKPI+DQDLKPANFFA+G+GHVNPSKAA+PGLVYDIQ DDYIPYLC LY 
Sbjct: 551 MTSADVRNPQGKPIMDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDDYIPYLCHLYT 610

Query: 61  DEEVSIIVHKTVICGLVPRIREGDLNYPSFSVEL-GGLQTFKRTVTNVGEANSVYSVMVE 120
           D +VSIIV K V C  VPRIREGDLNYPSF+V L  G QTF RTVTNVG+ANSVY  +VE
Sbjct: 611 DAQVSIIVRKQVTCSTVPRIREGDLNYPSFAVSLEAGSQTFNRTVTNVGDANSVYYPIVE 670

Query: 121 APLGVSMTVLPRKLVFSRVNQKLTFSVTFSRISSVKIIGEFGQGYLKWVSQKHVVRSPIS 180
           AP GVS+ V P  L FS++N+K+T+SVTFSRI  V+   EF +GYL WVS+K +VRSPIS
Sbjct: 671 APAGVSVKVTPSNLKFSKLNEKVTYSVTFSRIDFVRTTSEFSEGYLIWVSKKLMVRSPIS 730

Query: 181 VK 182
           VK
Sbjct: 731 VK 732

[Close]

BLAST of Lsi09G003420 vs. ExPASy TrEMBL
Match: A0A0A0KMQ7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G157280 PE=3 SV=1)

HSP 1 Score: 265.0 bits (676), Expect = 2.3e-67
Identity = 127/182 (69.78%), Postives = 150/182 (82.42%), Query Frame = 0


Query: 1   MTTANIKTPQGKPILDQDLKPANFFAIGAGHVNPSKAADPGLVYDIQSDDYIPYLCGLYK 60
           MT+A+++ PQGKPI+DQDLKPANFFA+G+GHVNPSKAA+PGLVYDIQ DDY+PYLC LY 
Sbjct: 551 MTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDDYVPYLCHLYT 610

Query: 61  DEEVSIIVHKTVICGLVPRIREGDLNYPSFSVELGG-LQTFKRTVTNVGEANSVYSVMVE 120
           D +VSIIV + V C  V RIREGDLNYPSF+V LG   Q F RTVTNVG+ANSVY  +V+
Sbjct: 611 DAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADSQAFNRTVTNVGDANSVYYAIVK 670

Query: 121 APLGVSMTVLPRKLVFSRVNQKLTFSVTFSRISSVKIIGEFGQGYLKWVSQKHVVRSPIS 180
           AP GVS+ V PR L FS++N+KLT+SVTFSRI  V+   EF +GYL WVS KH+VRSPIS
Sbjct: 671 APAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRTRSEFSEGYLIWVSNKHIVRSPIS 730

Query: 181 VK 182
           VK
Sbjct: 731 VK 732

[Close]

BLAST of Lsi09G003420 vs. NCBI nr
Match: KAA0042821.1 (subtilisin-like protease SBT1.7 [Cucumis melo var. makuwa])

HSP 1 Score: 327.4 bits (838), Expect = 7.9e-86
Identity = 158/184 (85.87%), Postives = 174/184 (94.57%), Query Frame = 0


Query: 1   MTTANIKTPQGKPILDQDLKPANFFAIGAGHVNPSKAADPGLVYDIQSDDYIPYLCGLYK 60
           MTTANIKTPQG+PI+DQDL+PANFFA+GAGHVNPSKAADPGLVYDIQ DDYIPYLCGLYK
Sbjct: 571 MTTANIKTPQGEPIVDQDLQPANFFAMGAGHVNPSKAADPGLVYDIQPDDYIPYLCGLYK 630

Query: 61  DEEVSIIVHKTVICGLVPRIREGDLNYPSFSVELGGLQTFKRTVTNVGEANSVYSVMVEA 120
           DEEVSIIVH+TVICGLVP IREGDLNYPSFSV LGGLQTFKRTVTNVGEANSVY+ +VEA
Sbjct: 631 DEEVSIIVHRTVICGLVPSIREGDLNYPSFSVALGGLQTFKRTVTNVGEANSVYTAIVEA 690

Query: 121 PLGVSMTVLPRKLVFSRVNQKLTFSVTFSRISSVKIIGEFGQGYLKWV--SQKHVVRSPI 180
           PLGVSMTV+PRKL FSRVNQ +TF+VTF+RI  VKI+GEFG+GYLKWV  S+K+VVRSP+
Sbjct: 691 PLGVSMTVMPRKLFFSRVNQTMTFTVTFNRIRWVKIVGEFGEGYLKWVSKSKKYVVRSPV 750

Query: 181 SVKF 183
           SVKF
Sbjct: 751 SVKF 754

[Close]

BLAST of Lsi09G003420 vs. NCBI nr
Match: XP_008437722.1 (PREDICTED: subtilisin-like protease SBT1.7 [Cucumis melo])

HSP 1 Score: 322.8 bits (826), Expect = 1.9e-84
Identity = 157/184 (85.33%), Postives = 172/184 (93.48%), Query Frame = 0


Query: 1   MTTANIKTPQGKPILDQDLKPANFFAIGAGHVNPSKAADPGLVYDIQSDDYIPYLCGLYK 60
           MTTANIKTPQG+PI DQDL+PANFFA+GAGHVNPSKAADPGLVYDIQ DDYIPYLCGLYK
Sbjct: 580 MTTANIKTPQGEPITDQDLQPANFFAMGAGHVNPSKAADPGLVYDIQPDDYIPYLCGLYK 639

Query: 61  DEEVSIIVHKTVICGLVPRIREGDLNYPSFSVELGGLQTFKRTVTNVGEANSVYSVMVEA 120
           DEEVSIIVH+TVICGLV  IREGDLNYPSFSV LGGLQTFKRTVTNVGEANSVY+ +VEA
Sbjct: 640 DEEVSIIVHRTVICGLVLSIREGDLNYPSFSVALGGLQTFKRTVTNVGEANSVYTAIVEA 699

Query: 121 PLGVSMTVLPRKLVFSRVNQKLTFSVTFSRISSVKIIGEFGQGYLKWV--SQKHVVRSPI 180
           PLGVSMTV+PRKL FSRVNQ +TF+VTF+RI  VKI+GEFG+GYLKWV  S+K+VVRSP+
Sbjct: 700 PLGVSMTVMPRKLFFSRVNQTMTFTVTFNRIRWVKIVGEFGEGYLKWVSKSKKYVVRSPV 759

Query: 181 SVKF 183
           SVKF
Sbjct: 760 SVKF 763

[Close]

BLAST of Lsi09G003420 vs. NCBI nr
Match: XP_008437177.1 (PREDICTED: subtilisin-like protease SBT1.7 [Cucumis melo])

HSP 1 Score: 267.7 bits (683), Expect = 7.4e-68
Identity = 130/182 (71.43%), Postives = 152/182 (83.52%), Query Frame = 0


Query: 1   MTTANIKTPQGKPILDQDLKPANFFAIGAGHVNPSKAADPGLVYDIQSDDYIPYLCGLYK 60
           MT+A+++ PQGKPI+DQDLKPANFFA+G+GHVNPSKAA+PGLVYDIQ DDYIPYLC LY 
Sbjct: 571 MTSADVRNPQGKPIMDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDDYIPYLCHLYT 630

Query: 61  DEEVSIIVHKTVICGLVPRIREGDLNYPSFSVELG-GLQTFKRTVTNVGEANSVYSVMVE 120
           D +VSIIV K V C  VPRIREGDLNYPSF+V LG G QTF RTVTNVG+ANSVY  +VE
Sbjct: 631 DAQVSIIVRKQVTCSTVPRIREGDLNYPSFAVSLGAGSQTFNRTVTNVGDANSVYYPIVE 690

Query: 121 APLGVSMTVLPRKLVFSRVNQKLTFSVTFSRISSVKIIGEFGQGYLKWVSQKHVVRSPIS 180
           AP GVS+ V P  L FS++N+K+T+SVTFSRI  V+   EF +GYL WVS+K +VRSPIS
Sbjct: 691 APAGVSVKVTPSNLKFSKLNEKVTYSVTFSRIDFVRTTSEFSEGYLIWVSKKLMVRSPIS 750

Query: 181 VK 182
           VK
Sbjct: 751 VK 752

[Close]

BLAST of Lsi09G003420 vs. NCBI nr
Match: XP_038906452.1 (subtilisin-like protease 4 [Benincasa hispida])

HSP 1 Score: 266.5 bits (680), Expect = 1.6e-67
Identity = 126/181 (69.61%), Postives = 149/181 (82.32%), Query Frame = 0


Query: 1   MTTANIKTPQGKPILDQDLKPANFFAIGAGHVNPSKAADPGLVYDIQSDDYIPYLCGLYK 60
           MT+A+++  QG+PI+DQDLKPANFFA+G+GHVNPSKAADPGLVYDIQ DDYIPYLCGLY 
Sbjct: 548 MTSADVRNRQGRPIVDQDLKPANFFAMGSGHVNPSKAADPGLVYDIQPDDYIPYLCGLYS 607

Query: 61  DEEVSIIVHKTVICGLVPRIREGDLNYPSFSVELGGLQTFKRTVTNVGEANSVYSVMVEA 120
           D +VSIIV + V C  + RIREGDLNYPSF+V LG  Q F RTVTNVG+ANS+YS +VEA
Sbjct: 608 DAQVSIIVRRKVTCSTISRIREGDLNYPSFAVTLGPSQMFNRTVTNVGDANSIYSAIVEA 667

Query: 121 PLGVSMTVLPRKLVFSRVNQKLTFSVTFSRISSVKIIGEFGQGYLKWVSQKHVVRSPISV 180
           P+GVS+ V P  L FSRV +K+T+SVTFSR   V+   EF +GYL WVS KH+VRSPISV
Sbjct: 668 PIGVSVKVTPNNLKFSRVIEKVTYSVTFSRDDLVRTTSEFSEGYLIWVSNKHMVRSPISV 727

Query: 181 K 182
           K
Sbjct: 728 K 728

[Close]

BLAST of Lsi09G003420 vs. NCBI nr
Match: KAA0042822.1 (subtilisin-like protease SBT1.7 [Cucumis melo var. makuwa])

HSP 1 Score: 265.4 bits (677), Expect = 3.7e-67
Identity = 129/182 (70.88%), Postives = 151/182 (82.97%), Query Frame = 0


Query: 1   MTTANIKTPQGKPILDQDLKPANFFAIGAGHVNPSKAADPGLVYDIQSDDYIPYLCGLYK 60
           MT+A+++ PQGKPI+DQDLKPANFFA+G+GHVNPSKAA+PGLVYDIQ DDYIPYLC LY 
Sbjct: 551 MTSADVRNPQGKPIMDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDDYIPYLCHLYT 610

Query: 61  DEEVSIIVHKTVICGLVPRIREGDLNYPSFSVEL-GGLQTFKRTVTNVGEANSVYSVMVE 120
           D +VSIIV K V C  VPRIREGDLNYPSF+V L  G QTF RTVTNVG+ANSVY  +VE
Sbjct: 611 DAQVSIIVRKQVTCSTVPRIREGDLNYPSFAVSLEAGSQTFNRTVTNVGDANSVYYPIVE 670

Query: 121 APLGVSMTVLPRKLVFSRVNQKLTFSVTFSRISSVKIIGEFGQGYLKWVSQKHVVRSPIS 180
           AP GVS+ V P  L FS++N+K+T+SVTFSRI  V+   EF +GYL WVS+K +VRSPIS
Sbjct: 671 APAGVSVKVTPSNLKFSKLNEKVTYSVTFSRIDFVRTTSEFSEGYLIWVSKKLMVRSPIS 730

Query: 181 VK 182
           VK
Sbjct: 731 VK 732

[Close]

BLAST of Lsi09G003420 vs. TAIR 10
Match: AT1G04110.1 (Subtilase family protein )

HSP 1 Score: 147.5 bits (371), Expect = 1.0e-35
Identity = 90/190 (47.37%), Postives = 116/190 (61.05%), Query Frame = 0


Query: 1   MTTANIKTPQGKPILDQDLKPANFFAIGAGHVNPSKAADPGLVYDIQSDDYIPYLCGL-Y 60
           MTTA++   QGK I D + KPA  FAIGAGHVNP KA +PGLVY+IQ  DYI YLC L +
Sbjct: 582 MTTADLYDRQGKAIKDGN-KPAGVFAIGAGHVNPQKAINPGLVYNIQPVDYITYLCTLGF 641

Query: 61  KDEEVSIIVHKTVICGLVPRIREG-DLNYPSFSVELGGLQT---FKRTVTNVGEANSVYS 120
              ++  I HK V C  + R   G  LNYPS +V     +T     R VTNVG  NS+YS
Sbjct: 642 TRSDILAITHKNVSCNGILRKNPGFSLNYPSIAVIFKRGKTTEMITRRVTNVGSPNSIYS 701

Query: 121 VMVEAPLGVSMTVLPRKLVFSRVNQKLTFSVTF--SRISSVKIIGEFGQGYLKWVSQKHV 180
           V V+AP G+ + V P++LVF  V+Q L++ V F   + +    +  F QG L WV+  ++
Sbjct: 702 VNVKAPEGIKVIVNPKRLVFKHVDQTLSYRVWFVLKKKNRGGKVASFAQGQLTWVNSHNL 761

[Close]

BLAST of Lsi09G003420 vs. TAIR 10
Match: AT1G01900.1 (subtilase family protein )

HSP 1 Score: 127.9 bits (320), Expect = 8.5e-30
Identity = 78/191 (40.84%), Postives = 107/191 (56.02%), Query Frame = 0


Query: 1   MTTANIKTPQGKPILDQDL----KPANFFAIGAGHVNPSKAADPGLVYDIQSDDYIPYLC 60
           MTTA I   + +PI D+        A  FA GAG+V+P++A DPGLVYD  + DY+ YLC
Sbjct: 582 MTTARITDNRNRPIGDRGAAGAESAATAFAFGAGNVDPTRAVDPGLVYDTSTVDYLNYLC 641

Query: 61  GL-YKDEEVSIIVHKTVICGL-VPRIREGDLNYPSFSVEL---GGLQT--FKRTVTNVGE 120
            L Y  E + +       C      +  GDLNYPSF+V L     L+T  +KRTVTNVG 
Sbjct: 642 SLNYTSERILLFSGTNYTCASNAVVLSPGDLNYPSFAVNLVNGANLKTVRYKRTVTNVGS 701

Query: 121 ANSVYSVMVEAPLGVSMTVLPRKLVFSRVNQKLTFSVTFSRISSVKIIGEFGQGYLKWVS 180
               Y V VE P GV + V P+ L F +  ++L+++VT+   +S +       G L W+ 
Sbjct: 702 PTCEYMVHVEEPKGVKVRVEPKVLKFQKARERLSYTVTYDAEAS-RNSSSSSFGVLVWIC 761

[Close]

BLAST of Lsi09G003420 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 127.1 bits (318), Expect = 1.5e-29
Identity = 74/187 (39.57%), Postives = 104/187 (55.61%), Query Frame = 0


Query: 1   MTTANIKTPQGKPILD-QDLKPANFFAIGAGHVNPSKAADPGLVYDIQSDDYIPYLCGL- 60
           MTTA +      P+ D  D   +N +A G+GHV+P KA  PGLVYDI +++YI +LC L 
Sbjct: 566 MTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLD 625

Query: 61  YKDEEVSIIVHKTVICGLVPRIREGDLNYPSFSVELGGLQT--FKRTVTNVGEANSVYSV 120
           Y  + +  IV +  +         G LNYPSFSV  GG +   + R VTNVG A+SVY V
Sbjct: 626 YTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSVLFGGKRVVRYTREVTNVGAASSVYKV 685

Query: 121 MVEAPLGVSMTVLPRKLVFSRVNQKLTFSVTFSRISSVKIIGEFGQGYLKWVSQKHVVRS 180
            V     V ++V P KL F  V +K  ++VTF     V +  +   G + W + +H VRS
Sbjct: 686 TVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAEFGSITWSNPQHEVRS 745

Query: 181 PISVKFN 184
           P++  +N
Sbjct: 746 PVAFSWN 752

[Close]

BLAST of Lsi09G003420 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 127.1 bits (318), Expect = 1.5e-29
Identity = 74/187 (39.57%), Postives = 110/187 (58.82%), Query Frame = 0


Query: 1   MTTANIKTPQGKPILD-QDLKPANFFAIGAGHVNPSKAADPGLVYDIQSDDYIPYLCGL- 60
           MTTA      GKP+LD    KP+  F  GAGHV+P+ A +PGL+YD+ ++DY+ +LC L 
Sbjct: 572 MTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALN 631

Query: 61  YKDEEVSIIVHKTVICGLVPRIREGDLNYPSFSVELGGLQTFK--RTVTNVGEANSVYSV 120
           Y   ++  +  +   C         DLNYPSF+V + G+  +K  RTVT+VG A + YSV
Sbjct: 632 YTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGT-YSV 691

Query: 121 MVEA-PLGVSMTVLPRKLVFSRVNQKLTFSVTFSRISSVKIIGEFGQGYLKWVSQKHVVR 180
            V +   GV ++V P  L F   N+K +++VTF+ + S K  G    G ++W   KHVV 
Sbjct: 692 KVTSETTGVKISVEPAVLNFKEANEKKSYTVTFT-VDSSKPSGSNSFGSIEWSDGKHVVG 751

Query: 181 SPISVKF 183
           SP+++ +
Sbjct: 752 SPVAISW 756

[Close]

BLAST of Lsi09G003420 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 125.6 bits (314), Expect = 4.2e-29
Identity = 74/191 (38.74%), Postives = 111/191 (58.12%), Query Frame = 0


Query: 1   MTTANIKTPQGKPILDQDL-KPANFFAIGAGHVNPSKAADPGLVYDIQSDDYIPYLCGL- 60
           MTT N+     + ++D+   K A  +  G+GH+N  +A +PGLVYDI +DDYI +LC + 
Sbjct: 568 MTTTNLVDNSNRSLIDESTGKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIG 627

Query: 61  YKDEEVSIIVHKTVICGLVPRIREGDLNYPS----FSVELGGL--QTFKRTVTNVGEANS 120
           Y  + + +I    V C    +   G+LNYPS    F     GL  +T  RT TNVG+A +
Sbjct: 628 YGPKTIQVITRTPVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEA 687

Query: 121 VYSVMVEAPLGVSMTVLPRKLVFSRVNQKLTFSVTFSRISSVKIIGEFGQ--GYLKWV-S 180
           VY   +E+P GV++TV P +LVF+   ++ +++VT +  +   ++GE G   G + W   
Sbjct: 688 VYRARIESPRGVTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDG 747

The following BLAST results are available for this feature:

BLAST of Lsi09G003420 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A9QY38	2.0e-55	61.20	Subtilisin-like protease 4 OS=Lotus japonicus OX=34305 GN=SBTM4 PE=2 SV=1	[more]
A9QY40	2.7e-49	54.59	Subtilisin-like protease 1 OS=Lotus japonicus OX=34305 GN=SBTM1 PE=2 SV=1	[more]
A9QY39	1.4e-48	55.98	Subtilisin-like protease 3 OS=Lotus japonicus OX=34305 GN=SBTM3 PE=2 SV=1	[more]
G7KEU7	3.0e-48	55.68	Subtilisin-like protease OS=Medicago truncatula OX=3880 GN=SBT PE=2 SV=1	[more]
A0A0M3R8G2	1.3e-43	50.82	Subtilisin-like protease OS=Petunia hybrida OX=4102 GN=SBT PE=2 SV=1	[more]

BLAST of Lsi09G003420 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TMM4	3.8e-86	85.87	Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_...	[more]
A0A1S3AUP1	9.4e-85	85.33	subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103483066 PE=3 SV=...	[more]
A0A1S3AT16	3.6e-68	71.43	subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103482683 PE=3 SV=...	[more]
A0A5A7THE4	1.8e-67	70.88	Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_...	[more]
A0A0A0KMQ7	2.3e-67	69.78	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G157280 PE=3 SV=1	[more]

BLAST of Lsi09G003420 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0042821.1	7.9e-86	85.87	subtilisin-like protease SBT1.7 [Cucumis melo var. makuwa]	[more]
XP_008437722.1	1.9e-84	85.33	PREDICTED: subtilisin-like protease SBT1.7 [Cucumis melo]	[more]
XP_008437177.1	7.4e-68	71.43	PREDICTED: subtilisin-like protease SBT1.7 [Cucumis melo]	[more]
XP_038906452.1	1.6e-67	69.61	subtilisin-like protease 4 [Benincasa hispida]	[more]
KAA0042822.1	3.7e-67	70.88	subtilisin-like protease SBT1.7 [Cucumis melo var. makuwa]	[more]

BLAST of Lsi09G003420 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G04110.1	1.0e-35	47.37	Subtilase family protein	[more]
AT1G01900.1	8.5e-30	40.84	subtilase family protein	[more]
AT2G05920.1	1.5e-29	39.57	Subtilase family protein	[more]
AT5G67360.1	1.5e-29	39.57	Subtilase family protein	[more]
AT4G34980.1	4.2e-29	38.74	subtilisin-like serine protease 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036852	Peptidase S8/S53 domain superfamily	GENE3D	3.40.50.200	Peptidase S8/S53 domain	coord: 1..51 e-value: 1.5E-10 score: 42.4
None	No IPR available	GENE3D	2.60.40.2310		coord: 55..182 e-value: 5.3E-36 score: 125.2
None	No IPR available	PANTHER	PTHR10795:SF557	SERINE PROTEINASE 1, PUTATIVE-RELATED	coord: 1..180
IPR041469	Subtilisin-like protease, fibronectin type-III domain	PFAM	PF17766	fn3_6	coord: 84..180 e-value: 3.2E-28 score: 97.8
IPR045051	Subtilisin-like protease	PANTHER	PTHR10795	PROPROTEIN CONVERTASE SUBTILISIN/KEXIN	coord: 1..180

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi09G003420.1	Lsi09G003420.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
biological_process	GO:0009610	response to symbiotic fungus
molecular_function	GO:0004252	serine-type endopeptidase activity