Lsi09G001720 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi09G001720
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionshort-chain dehydrogenase TIC 32, chloroplastic-like
Locationchr09: 1794538 .. 1797526 (-)
RNA-Seq ExpressionLsi09G001720
SyntenyLsi09G001720
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAATGACAAATATGTTATATCCACCCTTTCTTTTATTTCTCTCTCTCCTCCTCCATTATAAATTATAGTCCAAACCTCCTTCTCTTCTTTCAATCCATTCCCCTTGCTGAAATTGACACATGAAGAGCGGCGTGGCCTGCCATTTCTGACATATCCCATTTTTTTCATTACTCTTCCAAATATGCTCCTTTCCCTCAACTACCTCTCCGGCTCCCCCGGCCCCTCCGGCTTCGGCTCCAAATCCACCGCCGAACACGTCACCTCCTCCGCCTCCCCCTTCCTCCTCCCTTCCTTCACCGCCATCATCACCGGTAACTAACTCTTCTTTTAACTTCTTATTCTTTCCTACTAAAATGATAAAAGTGATTTTAACTATTTTATAGGCGCCACGTCGGGTATTGGAGCTGAGACGGCTCGGATTCTTGCCAAACGTGGGGCTCGTTTGATCCTCCCGTCGCGGAATCTCAAAGCCGCTGAGGAAACTAAGGCTCGGATCGAGTCTGAATACTCTGATTCACGAATCATTGTTATGTCACTTGATTTGAGCTCTTTGTCCTCTGTTAGAACCTTCGTTGCCCAATTTGAATCCCTCAATCTCCCGCTTAATCTCCTCATGTATAATCCGAAATCCTTTTTTTTTTTTTTTTAATTCTCTCTTTTTAATGGGATTTTAATTTTAATTTTGATTTTGATTTTCTAAAAAATAGAAACAACGCGGGGAAATTCTCGCACGAGCATGCAATATCGGAGGACGGGATCGAGATGACCTTCGCCACTAATTATTTGGGTAATTTAACTTTTTTTAAAAAATAATAAATTGTTTATAGAAAAAAAAATTGGAAAATATATTAATGTAATAAAATAATTTCAGGTCATTTTCTGTTGACGAAGCTGTTGGTGAATAAGATGGCGGAGACGGCGAAAGCGACGGGAGTTGAGGGGCGGATTGTCAACGTGTCGTCGAGCATTCACGGCTGGTTTTCCGGCGACATCATTAAATATCTCGGCCAGATAAGCCGAAATAAAAGGTGAATTTAATATTAAAAGGTTTTTTTTTCTTAATTTTCTTGGTATAAATATATATATTTTCTTGGGTTTCAATAGTTAATTTCCATCCTTATATTTGAAATCGTCTCCTCCACTTTTAACTTATTTTTTCATTTTTCATCTGTGTTTGGTCACTGTATTCTTAAATGACTACCACTTTGGTCATTTCATTAACTCTCGGTTTGAAATGAAAAAAAAAAAAAAATTCAGAATTTTTTTTGAATTTTTTTAAATTTTATTTACTCCAAATCCTACAAAATCCCACTATTTTAATTAGTTAGTTTTTTCCCCTTTTTACATAAAACCAAAATATCAAATTATTCTCTTTCTTGCCATATCAAATAATTCATTTGAAATCAAAGTAGCTGTTCTGTATGAAATTTCTCTTAGAATTTTATAGTTCTAAATTTTATCTGGTTAATTTTGGTCTCTATACAAAATCTTGTTTTATTTGGTCAGAGTTATTGAAAATTGATTATTTTGAAGAAATAACTATAACGATAACTCTTAAAAGTATACAAACTAAAATAAACATTTTTTAAATGAATAAAAGTATAAAGATGAAAATAAATAATTTAAAAAAATAGAAGGGGTTTGACTTGGGGAATATTGTATAAATAAATCTAATACAGTTAATGTGGGTGGATTCCCAGCAGAAATTACGATGCGACACGTGCTTACGCTCTCTCCAAGCTTGCTAACGTTCTGCATACCCACGAACTTGCTCGCCGATTGAAGGTTCTTTACATTATAATATTCTATTAATTATTTTTCTATTACTCTACTTTTACTCTTTTTTTTAATCCATTATCTTTAATCTTCATTAAAAATTTATTATTCTGTTTTTTTTTTCTTTTGATTTAGCAAATGGGAGCTAATGTTACTGTGAACTGCGTTCATCCGGGAATCGTCAGAACTCGACTTACTAGAGATCGTGAAGGTTTCATTACAGGTATGAAATTTCTTTACTTTTTTCTTTTAATTTAAACACTATACGAAAATTAAAAATAATTTAAAAAAAAATACTGCAGATTTGGTGTTCTTTATGGCTTCCAAGCTGTTGAAGACAATCCCTCAGGTAAACAAGCCAAACCTCTCATTAATTTTTTTTATTAAATTATAAATTTAAAAATAATTAAAATCATTTTGTTATGTTTTAATGGTATATATAGAAATTGCATTTAAAAGTGTAAAATTAAATATTAAATTATATTTTAATGGTATATATAGAAATTACATTTAAAAGTGTAAAATTAAATATTAAATTATTTTAAATGATAAAAGACTTATTTTAAAGTAGTTTCTAATGTGACAAATACCTTTTTTTAGTATTTCAATATCACTCCCAAACTTGTTTGTATTATGGTTGATGTTGTCAAAATGATGATATAATTAAATTTTTTTTTAGTTAATCGATAATTTAACTACCCATGTACTTTAAAGGTTAGATTAGGAGTTTAGTAGCCAAAATAAATTTGTCTCATTAAGTAGCATGCACTCCTCACTTTGAAGTTGGAGATTAAATCTTCTATTTGTTTGACTAAGTTTTAATTTAACCTATTTTAACCTCAATTTTGTTTTAAGAAATAAAAACCAAAAACCTTTTTATTTATTTCGAAGTTTACATTTTCCGTTTGTTTCCCGAGAAAAAAAGGCTATATATTAATAGAACTGACACACAGACCATTCTGATTTTCCTTCTTCCACAGGCGGCGGCAACGACATGCTACGTGGCGATGAACCCGAGGCTAAGCCACGTCACCGGCAAGTATTTCGCCGACTGCAACGAATCCTCTCCGTCCAAACTCGCCTCCAGTCCGGCCGAAGCCGCCCGTCTCTGGTCCGCCTCCGAGATCATGGTCAACGCAAACTCAAAACTCGTTTTCGATCCAAACAATGCCTTGGACTAATTTAGTTTCTATTAAAAATTAAAATAACTCC

mRNA sequence

AAAATGACAAATATGTTATATCCACCCTTTCTTTTATTTCTCTCTCTCCTCCTCCATTATAAATTATAGTCCAAACCTCCTTCTCTTCTTTCAATCCATTCCCCTTGCTGAAATTGACACATGAAGAGCGGCGTGGCCTGCCATTTCTGACATATCCCATTTTTTTCATTACTCTTCCAAATATGCTCCTTTCCCTCAACTACCTCTCCGGCTCCCCCGGCCCCTCCGGCTTCGGCTCCAAATCCACCGCCGAACACGTCACCTCCTCCGCCTCCCCCTTCCTCCTCCCTTCCTTCACCGCCATCATCACCGGCGCCACGTCGGGTATTGGAGCTGAGACGGCTCGGATTCTTGCCAAACGTGGGGCTCGTTTGATCCTCCCGTCGCGGAATCTCAAAGCCGCTGAGGAAACTAAGGCTCGGATCGAGTCTGAATACTCTGATTCACGAATCATTGTTATGTCACTTGATTTGAGCTCTTTGTCCTCTGTTAGAACCTTCGTTGCCCAATTTGAATCCCTCAATCTCCCGCTTAATCTCCTCATAAACAACGCGGGGAAATTCTCGCACGAGCATGCAATATCGGAGGACGGGATCGAGATGACCTTCGCCACTAATTATTTGGGTCATTTTCTGTTGACGAAGCTGTTGGTGAATAAGATGGCGGAGACGGCGAAAGCGACGGGAGTTGAGGGGCGGATTGTCAACGTGTCGTCGAGCATTCACGGCTGGTTTTCCGGCGACATCATTAAATATCTCGGCCAGATAAGCCGAAATAAAAGAAATTACGATGCGACACGTGCTTACGCTCTCTCCAAGCTTGCTAACGTTCTGCATACCCACGAACTTGCTCGCCGATTGAAGCAAATGGGAGCTAATGTTACTGTGAACTGCGTTCATCCGGGAATCGTCAGAACTCGACTTACTAGAGATCGTGAAGGTTTCATTACAGATTTGGTGTTCTTTATGGCTTCCAAGCTGTTGAAGACAATCCCTCAGGCGGCGGCAACGACATGCTACGTGGCGATGAACCCGAGGCTAAGCCACGTCACCGGCAAGTATTTCGCCGACTGCAACGAATCCTCTCCGTCCAAACTCGCCTCCAGTCCGGCCGAAGCCGCCCGTCTCTGGTCCGCCTCCGAGATCATGGTCAACGCAAACTCAAAACTCGTTTTCGATCCAAACAATGCCTTGGACTAATTTAGTTTCTATTAAAAATTAAAATAACTCC

Coding sequence (CDS)

ATGCTCCTTTCCCTCAACTACCTCTCCGGCTCCCCCGGCCCCTCCGGCTTCGGCTCCAAATCCACCGCCGAACACGTCACCTCCTCCGCCTCCCCCTTCCTCCTCCCTTCCTTCACCGCCATCATCACCGGCGCCACGTCGGGTATTGGAGCTGAGACGGCTCGGATTCTTGCCAAACGTGGGGCTCGTTTGATCCTCCCGTCGCGGAATCTCAAAGCCGCTGAGGAAACTAAGGCTCGGATCGAGTCTGAATACTCTGATTCACGAATCATTGTTATGTCACTTGATTTGAGCTCTTTGTCCTCTGTTAGAACCTTCGTTGCCCAATTTGAATCCCTCAATCTCCCGCTTAATCTCCTCATAAACAACGCGGGGAAATTCTCGCACGAGCATGCAATATCGGAGGACGGGATCGAGATGACCTTCGCCACTAATTATTTGGGTCATTTTCTGTTGACGAAGCTGTTGGTGAATAAGATGGCGGAGACGGCGAAAGCGACGGGAGTTGAGGGGCGGATTGTCAACGTGTCGTCGAGCATTCACGGCTGGTTTTCCGGCGACATCATTAAATATCTCGGCCAGATAAGCCGAAATAAAAGAAATTACGATGCGACACGTGCTTACGCTCTCTCCAAGCTTGCTAACGTTCTGCATACCCACGAACTTGCTCGCCGATTGAAGCAAATGGGAGCTAATGTTACTGTGAACTGCGTTCATCCGGGAATCGTCAGAACTCGACTTACTAGAGATCGTGAAGGTTTCATTACAGATTTGGTGTTCTTTATGGCTTCCAAGCTGTTGAAGACAATCCCTCAGGCGGCGGCAACGACATGCTACGTGGCGATGAACCCGAGGCTAAGCCACGTCACCGGCAAGTATTTCGCCGACTGCAACGAATCCTCTCCGTCCAAACTCGCCTCCAGTCCGGCCGAAGCCGCCCGTCTCTGGTCCGCCTCCGAGATCATGGTCAACGCAAACTCAAAACTCGTTTTCGATCCAAACAATGCCTTGGACTAA

Protein sequence

MLLSLNYLSGSPGPSGFGSKSTAEHVTSSASPFLLPSFTAIITGATSGIGAETARILAKRGARLILPSRNLKAAEETKARIESEYSDSRIIVMSLDLSSLSSVRTFVAQFESLNLPLNLLINNAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSIHGWFSGDIIKYLGQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMGANVTVNCVHPGIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVAMNPRLSHVTGKYFADCNESSPSKLASSPAEAARLWSASEIMVNANSKLVFDPNNALD
Homology
[Close]
BLAST of Lsi09G001720 vs. ExPASy Swiss-Prot
Match: A0A078IS66 (Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=TIC32A PE=2 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 1.1e-72
Identity = 158/320 (49.38%), Postives = 210/320 (65.62%), Query Frame = 0 

Query: 8   LSGSPGPSGFGSKSTAEHVTSSASPFLLPSFTAIITGATSGIGAETARILAKRGARLILP 67
           ++G  G SG+GS STAE VT S         TAIITG TSGIG E AR+L  RGA +I+ 
Sbjct: 7   VTGKAGKSGYGSASTAEDVTHSID---AKHLTAIITGGTSGIGLEAARVLGMRGAHVIIA 66

Query: 68  SRNLKAAEETKARIESEYSDSRIIVMSLDLSSLSSVRTFVAQFESLNLPLNLLINNAGKF 127
           SRN KAA ++K  I   Y ++RI  + LDLSS+ SVR+F+ QF +LN+PLN+LINNAG  
Sbjct: 67  SRNTKAANDSKEMILQMYPNARIDCLQLDLSSIKSVRSFIHQFLALNVPLNILINNAGVM 126

Query: 128 SHEHAISEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSIH--GWFS 187
                +SEDGIE  FATN++GHFLLT LL++KM  +A+ +G+EGRIVN+SS  H   +  
Sbjct: 127 FCPFQLSEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIVNLSSIAHTYTYTE 186

Query: 188 GDIIKYLGQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMGANVTVNCVHPGIVRT 247
           G +  Y+    R    Y   +AY  SKLAN+LH++ L+R+L++ G N+T+N VHPG++ T
Sbjct: 187 GIMFDYINDPDR----YSEKKAYGQSKLANLLHSNALSRKLQEEGVNITINSVHPGLITT 246

Query: 248 RLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVAMNPRLSHVTGKYFADCNESSPSKL 307
            L R   G    ++  M+  L K IPQ AATTCYVA++P L  VTGKYFADCN ++PS  
Sbjct: 247 NLFR-HSGLGMAVLKAMSFFLWKNIPQGAATTCYVALHPDLKDVTGKYFADCNVTTPSNF 306

Query: 308 ASSPAEAARLWSASEIMVNA 326
           A     A +LW  S  +V +
Sbjct: 307 AIDTTLADKLWDFSIKLVES 318

[Close]
BLAST of Lsi09G001720 vs. ExPASy Swiss-Prot
Match: A0A078ISJ6 (Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=TIC32B PE=3 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 7.3e-72
Identity = 156/320 (48.75%), Postives = 210/320 (65.62%), Query Frame = 0 

Query: 8   LSGSPGPSGFGSKSTAEHVTSSASPFLLPSFTAIITGATSGIGAETARILAKRGARLILP 67
           ++G  G SG+GS STAE VT S         TAIITG TSGIG E AR+L  RGA +I+ 
Sbjct: 7   VTGKAGKSGYGSASTAEDVTHSID---AKHLTAIITGGTSGIGLEAARVLGMRGAHVIIA 66

Query: 68  SRNLKAAEETKARIESEYSDSRIIVMSLDLSSLSSVRTFVAQFESLNLPLNLLINNAGKF 127
           +RN KAA ++K  I   Y ++RI  + LDLSS+ SVR+F+ QF +LN+PLN+LINNAG  
Sbjct: 67  ARNTKAANDSKEMILQMYPNARIDCLQLDLSSIKSVRSFIHQFLALNVPLNILINNAGVM 126

Query: 128 SHEHAISEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSIH--GWFS 187
                +SEDGIE  FATN++GHFLLT LL++KM  +A+ +G+EGRIVN+SS  H   +  
Sbjct: 127 FCPFQLSEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIVNLSSIAHTYTYTE 186

Query: 188 GDIIKYLGQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMGANVTVNCVHPGIVRT 247
           G +  Y+    R    Y   +AY  SKLAN+LH++ L+R+L++ G N+T+N VHPG++ T
Sbjct: 187 GIMFDYINDPDR----YSEKKAYGQSKLANLLHSNALSRKLQEEGVNITINSVHPGLITT 246

Query: 248 RLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVAMNPRLSHVTGKYFADCNESSPSKL 307
            L R   G    ++  M+  L K IPQ AATTCYVA++P L  VTGKYF DCN ++PS  
Sbjct: 247 NLFR-HSGLGMAVLKAMSFFLWKNIPQGAATTCYVALHPDLKGVTGKYFTDCNVTTPSNF 306

Query: 308 ASSPAEAARLWSASEIMVNA 326
           A     A +LW  S  +V++
Sbjct: 307 AIDTTLADKLWDFSIKLVDS 318

[Close]
BLAST of Lsi09G001720 vs. ExPASy Swiss-Prot
Match: A2RVM0 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC32 PE=2 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 4.6e-66
Identity = 140/318 (44.03%), Postives = 203/318 (63.84%), Query Frame = 0 

Query: 10  GSPGPSGFGSKSTAEHVTSSASPFLLPSFTAIITGATSGIGAETARILAKRGARLILPSR 69
           GS G SGF S+STAE VT           TAI+TGA+SGIG ETAR+L+ RG  +++  R
Sbjct: 5   GSKGASGFSSRSTAEEVTHGVDG---TGLTAIVTGASSGIGVETARVLSLRGVHVVMAVR 64

Query: 70  NLKAAEETKARIESEYSDSRIIVMSLDLSSLSSVRTFVAQFESLNLPLNLLINNAGKFSH 129
           N  +  + K  I  +   +++ VM LDLSS+ SVR F ++++S  LPLNLLINNAG  + 
Sbjct: 65  NTDSGAKVKEDIVKQVPGAKLDVMELDLSSMQSVRKFASEYKSTGLPLNLLINNAGIMAC 124

Query: 130 EHAISEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSIHGWFSGDII 189
              +S+D IE+ FATN+LGHFLLTKLL++ M  T++ +  EGRIVN+SS  H +   + +
Sbjct: 125 PFMLSKDNIELQFATNHLGHFLLTKLLLDTMKSTSRESKREGRIVNLSSEAHRFSYPEGV 184

Query: 190 KYLGQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMGANVTVNCVHPGIVRTRLTR 249
           ++      +K +Y + RAY  SKL NVLH +EL ++LK+ G N+T N +HPG + T L R
Sbjct: 185 RF--DKINDKSSYSSMRAYGQSKLCNVLHANELTKQLKEDGVNITANSLHPGAIMTNLGR 244

Query: 250 DREGFITDLVFFMASKLLKTIPQAAATTCYVAMNPRLSHVTGKYFADCNESSPSKLASSP 309
               ++   V  +A  +LK++PQ AATTCYVA+NP+++ V+G+YF D N + P  L    
Sbjct: 245 YFNPYLAVAVGAVAKYILKSVPQGAATTCYVALNPQVAGVSGEYFQDSNIAKPLPLVKDT 304

Query: 310 AEAARLWSASEIMVNANS 328
             A ++W  S  + ++ S
Sbjct: 305 ELAKKVWDFSTKLTDSQS 317

[Close]
BLAST of Lsi09G001720 vs. ExPASy Swiss-Prot
Match: Q6RVV4 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC32 PE=1 SV=1)

HSP 1 Score: 230.7 bits (587), Expect = 2.4e-59
Identity = 133/311 (42.77%), Postives = 191/311 (61.41%), Query Frame = 0 

Query: 9   SGSPGPSGFGSKSTAEHVTSSASPFLLPSFTAIITGATSGIGAETARILAKRGARLILPS 68
           S   G SGF   STAE VT           TAI+TGA+SGIGAET R+LA RGA +I+  
Sbjct: 5   SSKKGVSGFSGSSTAEQVTHGID---ATGLTAIVTGASSGIGAETTRVLALRGAHVIMGV 64

Query: 69  RNLKAAEETKARIESEYSDSRIIVMSLDLSSLSSVRTFVAQFESLNLPLNLLINNAGKFS 128
           RN+ AA++ K  I  +   +++  + LDLSSL SV+ F ++F S   PLN+LINNAG  +
Sbjct: 65  RNMVAAKDVKDTILKDIPSAKVDAIELDLSSLDSVKKFASEFNSSGRPLNILINNAGIMA 124

Query: 129 HEHAISEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSIHGWFSGDI 188
               +S+D IE+ FATN++GHFLLT LL++ M +T + +  EGRIVNV+S  H +   + 
Sbjct: 125 CPFKLSKDNIELQFATNHIGHFLLTNLLLDTMKKTTRESKKEGRIVNVASEAHRFAYPEG 184

Query: 189 IKYLGQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMGANVTVNCVHPGIVRTRLT 248
           I++      ++ +Y+  RAY  SKLANVLH ++L + LK+ G N+T N +HPG + T L 
Sbjct: 185 IRF--DKINDQSSYNNWRAYGQSKLANVLHANQLTKHLKEDGVNITANSLHPGTIVTNLF 244

Query: 249 RDREGFITDLVFFMASKLLKTIPQAAATTCYVAMNPRLSHVTGKYFADCNESSPSKLASS 308
           R     +  L+  +   +LK + Q AATTCYVA++P++  V+G+YF+D N    +     
Sbjct: 245 RHNSA-VNGLINVIGKLVLKNVQQGAATTCYVALHPQVKGVSGEYFSDSNVYKTTPHGKD 304

Query: 309 PAEAARLWSAS 320
              A +LW  S
Sbjct: 305 VDLAKKLWDFS 309

[Close]
BLAST of Lsi09G001720 vs. ExPASy Swiss-Prot
Match: P59837 (Retinol dehydrogenase 12 OS=Bos taurus OX=9913 GN=RDH12 PE=2 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 1.9e-43
Identity = 115/283 (40.64%), Postives = 167/283 (59.01%), Query Frame = 0 

Query: 41  IITGATSGIGAETARILAKRGARLILPSRNLKAAEETKARIESEYSDSRIIVMSLDLSSL 100
           +ITGA +GIG ETAR LA+RGAR+ +  R++   E   + I+++  +S+++V  LDLS  
Sbjct: 43  VITGANTGIGKETARELARRGARVYIACRDVLKGESAASEIQADTKNSQVLVRKLDLSDT 102

Query: 101 SSVRTFVAQFESLNLPLNLLINNAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNKM 160
            S+R F   F +    L++LINNAG     ++ + DG E   A N+LGHFLLT LL+ ++
Sbjct: 103 KSIRAFAEGFLAEEKQLHILINNAGVMLCPYSKTADGFETHLAVNHLGHFLLTHLLLGRL 162

Query: 161 AETAKATGVEGRIVNVSSSIHGWFSGDIIKYLGQIS----RNKRNYDATRAYALSKLANV 220
            E+A A     R+VN+SS  H         +LG+I     +  + Y+   AY  SKLANV
Sbjct: 163 KESAPA-----RVVNLSSVAH---------HLGKIRFHDLQGDKYYNLGFAYCHSKLANV 222

Query: 221 LHTHELARRLKQMGANVTVNCVHPGIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAAT 280
           L T ELA+RLK  G  VT   VHPGIVR++L   R  F+  L++ + S  LKT  + A T
Sbjct: 223 LFTRELAKRLK--GTGVTTYAVHPGIVRSKLV--RHSFLLCLLWRLFSPFLKTTWEGAQT 282

Query: 281 TCYVAMNPRLSHVTGKYFADCNESSPSKLASSPAEAARLWSAS 320
           + + A+   L  ++GKYF+DC ++  S  A +   A RLW+ S
Sbjct: 283 SLHCALAEGLEPLSGKYFSDCKKTWVSPRARNNKTAERLWNVS 307

[Close]
BLAST of Lsi09G001720 vs. ExPASy TrEMBL
Match: A0A5D3C462 (Short-chain dehydrogenase TIC 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G001850 PE=3 SV=1)

HSP 1 Score: 601.3 bits (1549), Expect = 2.5e-168
Identity = 315/338 (93.20%), Postives = 323/338 (95.56%), Query Frame = 0 

Query: 1   MLLSLNYLSGSPGPSGFGSKSTAEHVTSSASPFLLPSFTAIITGATSGIGAETARILAKR 60
           MLLS NYLSGSPGPSGFGSKSTAEHVTSS    L PSFTAIITGATSGIGAETARILAKR
Sbjct: 1   MLLSFNYLSGSPGPSGFGSKSTAEHVTSS----LPPSFTAIITGATSGIGAETARILAKR 60

Query: 61  GARLILPSRNLKAAEETKARIESEYSDSRIIVMSLDLSSLSSVRTFVAQFESLNLPLNLL 120
           GARLILPSRNLKAAEETKARIESE SDS IIVMSLDLSSLSSVRTFV+QFESLNLPLNLL
Sbjct: 61  GARLILPSRNLKAAEETKARIESECSDSEIIVMSLDLSSLSSVRTFVSQFESLNLPLNLL 120

Query: 121 INNAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSI 180
           INNAGKFSHEHAI+EDGIEMTFATNYLGHFLLTKLLVNKM ETAK TGVEGRIVNVSSSI
Sbjct: 121 INNAGKFSHEHAITEDGIEMTFATNYLGHFLLTKLLVNKMVETAKVTGVEGRIVNVSSSI 180

Query: 181 HGWFSGDIIKYLGQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMGANVTVNCVHP 240
           H WFSGDI+KYLGQISRNKRNYDATRAYALSKLANVLHTHELARR KQMGANVTVNCVHP
Sbjct: 181 HSWFSGDILKYLGQISRNKRNYDATRAYALSKLANVLHTHELARRFKQMGANVTVNCVHP 240

Query: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVAMNPRLSHVTGKYFADCNES 300
           GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVA NPRL HVTGKYF DCNES
Sbjct: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATNPRLRHVTGKYFVDCNES 300

Query: 301 SPSKLASSPAEAARLWSASEIMVNANSKLVFDPNNALD 339
           SPSKLA+SP+EAARLWSASEIMVNA+SKL+FDPNNAL+
Sbjct: 301 SPSKLAASPSEAARLWSASEIMVNASSKLLFDPNNALE 334

[Close]
BLAST of Lsi09G001720 vs. ExPASy TrEMBL
Match: A0A0A0KKE2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G149870 PE=3 SV=1)

HSP 1 Score: 598.6 bits (1542), Expect = 1.6e-167
Identity = 315/339 (92.92%), Postives = 323/339 (95.28%), Query Frame = 0 

Query: 1   MLLSLNYLSGSPGPSGFGSKSTAEHVTSSASPFLLPSFTAIITGATSGIGAETARILAKR 60
           MLLS NYLSGSPGPSGFGSKSTAEHVT+S    L PSFTAIITGATSGIGAETARILAKR
Sbjct: 1   MLLSFNYLSGSPGPSGFGSKSTAEHVTAS----LPPSFTAIITGATSGIGAETARILAKR 60

Query: 61  GARLILPSRNLKAAEETKARIESEYSDSRIIVMSLDLSSLSSVRTFVAQFESLNLPLNLL 120
           GARLILPSRN+KAAEETKARIESE SDS IIVMSLDLSSLSSVRTFV+QFESLNLPLNLL
Sbjct: 61  GARLILPSRNIKAAEETKARIESECSDSEIIVMSLDLSSLSSVRTFVSQFESLNLPLNLL 120

Query: 121 INNAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSI 180
           INNAGKFSHEHAI+EDGIEMTFATNYLGHFLLTKLLVNKM ETAK TGVEGRIVNVSSSI
Sbjct: 121 INNAGKFSHEHAITEDGIEMTFATNYLGHFLLTKLLVNKMVETAKVTGVEGRIVNVSSSI 180

Query: 181 HGWFSGDIIKYLGQISRNK-RNYDATRAYALSKLANVLHTHELARRLKQMGANVTVNCVH 240
           HGWFSGDI+KYLGQISRNK RNYDATRAYALSKLANVLHTHELARR KQMGANVTVNCVH
Sbjct: 181 HGWFSGDILKYLGQISRNKSRNYDATRAYALSKLANVLHTHELARRFKQMGANVTVNCVH 240

Query: 241 PGIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVAMNPRLSHVTGKYFADCNE 300
           PGIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVA NPRL HVTGKYF DCNE
Sbjct: 241 PGIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATNPRLRHVTGKYFVDCNE 300

Query: 301 SSPSKLASSPAEAARLWSASEIMVNANSKLVFDPNNALD 339
           SSPSKLA SP+EAARLWSASEIMVNANSKL+FDPNNAL+
Sbjct: 301 SSPSKLAGSPSEAARLWSASEIMVNANSKLLFDPNNALE 335

[Close]
BLAST of Lsi09G001720 vs. ExPASy TrEMBL
Match: A0A6J1I2S6 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111469067 PE=3 SV=1)

HSP 1 Score: 575.1 bits (1481), Expect = 1.9e-160
Identity = 301/338 (89.05%), Postives = 318/338 (94.08%), Query Frame = 0 

Query: 1   MLLSLNYLSGSPGPSGFGSKSTAEHVTSSASPFLLPSFTAIITGATSGIGAETARILAKR 60
           ML S+ YL+GSPGPSGFGSKS+AEHVT+SA    LPS TAIITGATSGIGAETAR+LAKR
Sbjct: 1   MLHSIKYLAGSPGPSGFGSKSSAEHVTASA--LHLPSITAIITGATSGIGAETARVLAKR 60

Query: 61  GARLILPSRNLKAAEETKARIESEYSDSRIIVMSLDLSSLSSVRTFVAQFESLNLPLNLL 120
           GARLILPSRNLKAAEE KARIE+E S S II M+LDLSSL+SVRTFVAQFESLNLPLNLL
Sbjct: 61  GARLILPSRNLKAAEEAKARIEAECSGSEIIAMALDLSSLASVRTFVAQFESLNLPLNLL 120

Query: 121 INNAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSI 180
           INNAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVN M ETAKATG++GRIVNVSSSI
Sbjct: 121 INNAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNTMVETAKATGIQGRIVNVSSSI 180

Query: 181 HGWFSGDIIKYLGQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMGANVTVNCVHP 240
           HGWFSGDIIKYLGQISRNK NYDATRAYALSKLANVLHTHELARRLK+MGANVTVNCVHP
Sbjct: 181 HGWFSGDIIKYLGQISRNKWNYDATRAYALSKLANVLHTHELARRLKKMGANVTVNCVHP 240

Query: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVAMNPRLSHVTGKYFADCNES 300
           GIVRTRLTRDREGFITDLVFF+ SKLLKTIPQAAATTCYVA NPRLS+VTGKYFADCNE+
Sbjct: 241 GIVRTRLTRDREGFITDLVFFITSKLLKTIPQAAATTCYVAANPRLSNVTGKYFADCNEA 300

Query: 301 SPSKLASSPAEAARLWSASEIMVNANSKLVFDPNNALD 339
           SPSKLA+S  EAARLWSASEIMVN NSKLVF+PNNAL+
Sbjct: 301 SPSKLAASLTEAARLWSASEIMVNTNSKLVFNPNNALE 336

[Close]
BLAST of Lsi09G001720 vs. ExPASy TrEMBL
Match: A0A6J1HY34 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111469067 PE=3 SV=1)

HSP 1 Score: 573.5 bits (1477), Expect = 5.6e-160
Identity = 302/339 (89.09%), Postives = 319/339 (94.10%), Query Frame = 0 

Query: 1   MLLSLNYLSGSPGPSGFGSKSTAEHVTSSASPFLLPSFTAIITGATSGIGAETARILAKR 60
           ML S+ YL+GSPGPSGFGSKS+AEHVT+SA    LPS TAIITGATSGIGAETAR+LAKR
Sbjct: 1   MLHSIKYLAGSPGPSGFGSKSSAEHVTASA--LHLPSITAIITGATSGIGAETARVLAKR 60

Query: 61  GARLILPSRNLKAAEETKARIESEYSDSRIIVMSLDLSSLSSVRTFVAQFESLNLPLNLL 120
           GARLILPSRNLKAAEE KARIE+E S S II M+LDLSSL+SVRTFVAQFESLNLPLNLL
Sbjct: 61  GARLILPSRNLKAAEEAKARIEAECSGSEIIAMALDLSSLASVRTFVAQFESLNLPLNLL 120

Query: 121 INNAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSI 180
           INNAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVN M ETAKATG++GRIVNVSSSI
Sbjct: 121 INNAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNTMVETAKATGIQGRIVNVSSSI 180

Query: 181 HGWFSGDIIKYLGQISRNK-RNYDATRAYALSKLANVLHTHELARRLKQMGANVTVNCVH 240
           HGWFSGDIIKYLGQISRNK RNYDATRAYALSKLANVLHTHELARRLK+MGANVTVNCVH
Sbjct: 181 HGWFSGDIIKYLGQISRNKCRNYDATRAYALSKLANVLHTHELARRLKKMGANVTVNCVH 240

Query: 241 PGIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVAMNPRLSHVTGKYFADCNE 300
           PGIVRTRLTRDREGFITDLVFF+ SKLLKTIPQAAATTCYVA NPRLS+VTGKYFADCNE
Sbjct: 241 PGIVRTRLTRDREGFITDLVFFITSKLLKTIPQAAATTCYVAANPRLSNVTGKYFADCNE 300

Query: 301 SSPSKLASSPAEAARLWSASEIMVNANSKLVFDPNNALD 339
           +SPSKLA+S  EAARLWSASEIMVN NSKLVF+PNNAL+
Sbjct: 301 ASPSKLAASLTEAARLWSASEIMVNTNSKLVFNPNNALE 337

[Close]
BLAST of Lsi09G001720 vs. ExPASy TrEMBL
Match: A0A6J1EMX3 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111435812 PE=3 SV=1)

HSP 1 Score: 572.0 bits (1473), Expect = 1.6e-159
Identity = 298/338 (88.17%), Postives = 316/338 (93.49%), Query Frame = 0 

Query: 1   MLLSLNYLSGSPGPSGFGSKSTAEHVTSSASPFLLPSFTAIITGATSGIGAETARILAKR 60
           ML S+ YL+GSPGPSGFGSKS+AEHVT+SA    LPS TAIITGATSGIGAETAR+LAKR
Sbjct: 1   MLHSIKYLAGSPGPSGFGSKSSAEHVTASA--LHLPSITAIITGATSGIGAETARVLAKR 60

Query: 61  GARLILPSRNLKAAEETKARIESEYSDSRIIVMSLDLSSLSSVRTFVAQFESLNLPLNLL 120
           GARLILPSRNLKAAEE KARIE+E S S II M+LDLSSL+SVRTFVAQFESLNLPLNLL
Sbjct: 61  GARLILPSRNLKAAEEAKARIEAECSGSEIIAMALDLSSLASVRTFVAQFESLNLPLNLL 120

Query: 121 INNAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSI 180
           +NNAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVN M ET KATG++GRIVNVSSSI
Sbjct: 121 VNNAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNTMVETTKATGIQGRIVNVSSSI 180

Query: 181 HGWFSGDIIKYLGQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMGANVTVNCVHP 240
           HGWFSGDIIKYLGQISRNK NYDATRAYALSKLANVLHTHELARRLK+ GAN+TVNCVHP
Sbjct: 181 HGWFSGDIIKYLGQISRNKWNYDATRAYALSKLANVLHTHELARRLKKTGANITVNCVHP 240

Query: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVAMNPRLSHVTGKYFADCNES 300
           GIVRTRLTRDREGFITDLVFF+ SKLLKTIPQAAATTCYVA NPRLS+VTGKYFADCNE+
Sbjct: 241 GIVRTRLTRDREGFITDLVFFITSKLLKTIPQAAATTCYVAANPRLSNVTGKYFADCNEA 300

Query: 301 SPSKLASSPAEAARLWSASEIMVNANSKLVFDPNNALD 339
           SPSKLA+S  EAARLWSASEIMVN NSKLVF+PNNALD
Sbjct: 301 SPSKLAASLTEAARLWSASEIMVNTNSKLVFNPNNALD 336

[Close]
BLAST of Lsi09G001720 vs. NCBI nr
Match: XP_038875366.1 (short-chain dehydrogenase TIC 32 A, chloroplastic-like isoform X2 [Benincasa hispida])

HSP 1 Score: 627.1 bits (1616), Expect = 8.9e-176
Identity = 329/338 (97.34%), Postives = 331/338 (97.93%), Query Frame = 0 

Query: 1   MLLSLNYLSGSPGPSGFGSKSTAEHVTSSASPFLLPSFTAIITGATSGIGAETARILAKR 60
           MLLSLNYLSGSPGPSGFGSKSTAEHVTSSA PFLLPSFTAIITGATSGIGAETAR+LAKR
Sbjct: 1   MLLSLNYLSGSPGPSGFGSKSTAEHVTSSA-PFLLPSFTAIITGATSGIGAETARVLAKR 60

Query: 61  GARLILPSRNLKAAEETKARIESEYSDSRIIVMSLDLSSLSSVRTFVAQFESLNLPLNLL 120
           GARLILPSRNLKAAEETKARIESE SDS IIVMSLDLSSLSSVRTFVAQFESLNLPLNLL
Sbjct: 61  GARLILPSRNLKAAEETKARIESECSDSEIIVMSLDLSSLSSVRTFVAQFESLNLPLNLL 120

Query: 121 INNAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSI 180
           INNAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNKM ETAKATGVEGRIVNVSSSI
Sbjct: 121 INNAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNKMVETAKATGVEGRIVNVSSSI 180

Query: 181 HGWFSGDIIKYLGQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMGANVTVNCVHP 240
           HGWFSGDIIKYLGQISRNKRNYDATRAYALSKLANVLHTHEL RRLKQMGANVTVNCVHP
Sbjct: 181 HGWFSGDIIKYLGQISRNKRNYDATRAYALSKLANVLHTHELGRRLKQMGANVTVNCVHP 240

Query: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVAMNPRLSHVTGKYFADCNES 300
           GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVA NPRLSHVTGKYFADCNES
Sbjct: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATNPRLSHVTGKYFADCNES 300

Query: 301 SPSKLASSPAEAARLWSASEIMVNANSKLVFDPNNALD 339
           SPSKLA+SP EAARLWSASEIMVNANSKLVFDPNNALD
Sbjct: 301 SPSKLAASPTEAARLWSASEIMVNANSKLVFDPNNALD 337

[Close]
BLAST of Lsi09G001720 vs. NCBI nr
Match: XP_038875365.1 (short-chain dehydrogenase TIC 32 A, chloroplastic-like isoform X1 [Benincasa hispida])

HSP 1 Score: 622.5 bits (1604), Expect = 2.2e-174
Identity = 329/339 (97.05%), Postives = 331/339 (97.64%), Query Frame = 0 

Query: 1   MLLSLNYLSGSPGPSGFGSKSTAEHVTSSASPFLLPSFTAIITGATSGIGAETARILAKR 60
           MLLSLNYLSGSPGPSGFGSKSTAEHVTSSA PFLLPSFTAIITGATSGIGAETAR+LAKR
Sbjct: 1   MLLSLNYLSGSPGPSGFGSKSTAEHVTSSA-PFLLPSFTAIITGATSGIGAETARVLAKR 60

Query: 61  GARLILPSRNLKAAEETKARIESEYSDSRIIVMSLDLSSLSSVRTFVAQFESLNLPLNLL 120
           GARLILPSRNLKAAEETKARIESE SDS IIVMSLDLSSLSSVRTFVAQFESLNLPLNLL
Sbjct: 61  GARLILPSRNLKAAEETKARIESECSDSEIIVMSLDLSSLSSVRTFVAQFESLNLPLNLL 120

Query: 121 INNAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSI 180
           INNAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNKM ETAKATGVEGRIVNVSSSI
Sbjct: 121 INNAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNKMVETAKATGVEGRIVNVSSSI 180

Query: 181 HGWFSGDIIKYLGQISRNK-RNYDATRAYALSKLANVLHTHELARRLKQMGANVTVNCVH 240
           HGWFSGDIIKYLGQISRNK RNYDATRAYALSKLANVLHTHEL RRLKQMGANVTVNCVH
Sbjct: 181 HGWFSGDIIKYLGQISRNKSRNYDATRAYALSKLANVLHTHELGRRLKQMGANVTVNCVH 240

Query: 241 PGIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVAMNPRLSHVTGKYFADCNE 300
           PGIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVA NPRLSHVTGKYFADCNE
Sbjct: 241 PGIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATNPRLSHVTGKYFADCNE 300

Query: 301 SSPSKLASSPAEAARLWSASEIMVNANSKLVFDPNNALD 339
           SSPSKLA+SP EAARLWSASEIMVNANSKLVFDPNNALD
Sbjct: 301 SSPSKLAASPTEAARLWSASEIMVNANSKLVFDPNNALD 338

[Close]
BLAST of Lsi09G001720 vs. NCBI nr
Match: XP_004143954.1 (short-chain dehydrogenase TIC 32 A, chloroplastic isoform X2 [Cucumis sativus])

HSP 1 Score: 603.2 bits (1554), Expect = 1.4e-168
Identity = 315/338 (93.20%), Postives = 323/338 (95.56%), Query Frame = 0 

Query: 1   MLLSLNYLSGSPGPSGFGSKSTAEHVTSSASPFLLPSFTAIITGATSGIGAETARILAKR 60
           MLLS NYLSGSPGPSGFGSKSTAEHVT+S    L PSFTAIITGATSGIGAETARILAKR
Sbjct: 1   MLLSFNYLSGSPGPSGFGSKSTAEHVTAS----LPPSFTAIITGATSGIGAETARILAKR 60

Query: 61  GARLILPSRNLKAAEETKARIESEYSDSRIIVMSLDLSSLSSVRTFVAQFESLNLPLNLL 120
           GARLILPSRN+KAAEETKARIESE SDS IIVMSLDLSSLSSVRTFV+QFESLNLPLNLL
Sbjct: 61  GARLILPSRNIKAAEETKARIESECSDSEIIVMSLDLSSLSSVRTFVSQFESLNLPLNLL 120

Query: 121 INNAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSI 180
           INNAGKFSHEHAI+EDGIEMTFATNYLGHFLLTKLLVNKM ETAK TGVEGRIVNVSSSI
Sbjct: 121 INNAGKFSHEHAITEDGIEMTFATNYLGHFLLTKLLVNKMVETAKVTGVEGRIVNVSSSI 180

Query: 181 HGWFSGDIIKYLGQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMGANVTVNCVHP 240
           HGWFSGDI+KYLGQISRNKRNYDATRAYALSKLANVLHTHELARR KQMGANVTVNCVHP
Sbjct: 181 HGWFSGDILKYLGQISRNKRNYDATRAYALSKLANVLHTHELARRFKQMGANVTVNCVHP 240

Query: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVAMNPRLSHVTGKYFADCNES 300
           GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVA NPRL HVTGKYF DCNES
Sbjct: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATNPRLRHVTGKYFVDCNES 300

Query: 301 SPSKLASSPAEAARLWSASEIMVNANSKLVFDPNNALD 339
           SPSKLA SP+EAARLWSASEIMVNANSKL+FDPNNAL+
Sbjct: 301 SPSKLAGSPSEAARLWSASEIMVNANSKLLFDPNNALE 334

[Close]
BLAST of Lsi09G001720 vs. NCBI nr
Match: TYK06084.1 (short-chain dehydrogenase TIC 32 [Cucumis melo var. makuwa])

HSP 1 Score: 601.3 bits (1549), Expect = 5.2e-168
Identity = 315/338 (93.20%), Postives = 323/338 (95.56%), Query Frame = 0 

Query: 1   MLLSLNYLSGSPGPSGFGSKSTAEHVTSSASPFLLPSFTAIITGATSGIGAETARILAKR 60
           MLLS NYLSGSPGPSGFGSKSTAEHVTSS    L PSFTAIITGATSGIGAETARILAKR
Sbjct: 1   MLLSFNYLSGSPGPSGFGSKSTAEHVTSS----LPPSFTAIITGATSGIGAETARILAKR 60

Query: 61  GARLILPSRNLKAAEETKARIESEYSDSRIIVMSLDLSSLSSVRTFVAQFESLNLPLNLL 120
           GARLILPSRNLKAAEETKARIESE SDS IIVMSLDLSSLSSVRTFV+QFESLNLPLNLL
Sbjct: 61  GARLILPSRNLKAAEETKARIESECSDSEIIVMSLDLSSLSSVRTFVSQFESLNLPLNLL 120

Query: 121 INNAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSI 180
           INNAGKFSHEHAI+EDGIEMTFATNYLGHFLLTKLLVNKM ETAK TGVEGRIVNVSSSI
Sbjct: 121 INNAGKFSHEHAITEDGIEMTFATNYLGHFLLTKLLVNKMVETAKVTGVEGRIVNVSSSI 180

Query: 181 HGWFSGDIIKYLGQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMGANVTVNCVHP 240
           H WFSGDI+KYLGQISRNKRNYDATRAYALSKLANVLHTHELARR KQMGANVTVNCVHP
Sbjct: 181 HSWFSGDILKYLGQISRNKRNYDATRAYALSKLANVLHTHELARRFKQMGANVTVNCVHP 240

Query: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVAMNPRLSHVTGKYFADCNES 300
           GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVA NPRL HVTGKYF DCNES
Sbjct: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATNPRLRHVTGKYFVDCNES 300

Query: 301 SPSKLASSPAEAARLWSASEIMVNANSKLVFDPNNALD 339
           SPSKLA+SP+EAARLWSASEIMVNA+SKL+FDPNNAL+
Sbjct: 301 SPSKLAASPSEAARLWSASEIMVNASSKLLFDPNNALE 334

[Close]
BLAST of Lsi09G001720 vs. NCBI nr
Match: XP_011654698.1 (short-chain dehydrogenase TIC 32 A, chloroplastic isoform X1 [Cucumis sativus] >KGN50018.1 hypothetical protein Csa_000365 [Cucumis sativus])

HSP 1 Score: 598.6 bits (1542), Expect = 3.4e-167
Identity = 315/339 (92.92%), Postives = 323/339 (95.28%), Query Frame = 0 

Query: 1   MLLSLNYLSGSPGPSGFGSKSTAEHVTSSASPFLLPSFTAIITGATSGIGAETARILAKR 60
           MLLS NYLSGSPGPSGFGSKSTAEHVT+S    L PSFTAIITGATSGIGAETARILAKR
Sbjct: 1   MLLSFNYLSGSPGPSGFGSKSTAEHVTAS----LPPSFTAIITGATSGIGAETARILAKR 60

Query: 61  GARLILPSRNLKAAEETKARIESEYSDSRIIVMSLDLSSLSSVRTFVAQFESLNLPLNLL 120
           GARLILPSRN+KAAEETKARIESE SDS IIVMSLDLSSLSSVRTFV+QFESLNLPLNLL
Sbjct: 61  GARLILPSRNIKAAEETKARIESECSDSEIIVMSLDLSSLSSVRTFVSQFESLNLPLNLL 120

Query: 121 INNAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSI 180
           INNAGKFSHEHAI+EDGIEMTFATNYLGHFLLTKLLVNKM ETAK TGVEGRIVNVSSSI
Sbjct: 121 INNAGKFSHEHAITEDGIEMTFATNYLGHFLLTKLLVNKMVETAKVTGVEGRIVNVSSSI 180

Query: 181 HGWFSGDIIKYLGQISRNK-RNYDATRAYALSKLANVLHTHELARRLKQMGANVTVNCVH 240
           HGWFSGDI+KYLGQISRNK RNYDATRAYALSKLANVLHTHELARR KQMGANVTVNCVH
Sbjct: 181 HGWFSGDILKYLGQISRNKSRNYDATRAYALSKLANVLHTHELARRFKQMGANVTVNCVH 240

Query: 241 PGIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVAMNPRLSHVTGKYFADCNE 300
           PGIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVA NPRL HVTGKYF DCNE
Sbjct: 241 PGIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATNPRLRHVTGKYFVDCNE 300

Query: 301 SSPSKLASSPAEAARLWSASEIMVNANSKLVFDPNNALD 339
           SSPSKLA SP+EAARLWSASEIMVNANSKL+FDPNNAL+
Sbjct: 301 SSPSKLAGSPSEAARLWSASEIMVNANSKLLFDPNNALE 335

[Close]
BLAST of Lsi09G001720 vs. TAIR 10
Match: AT4G24050.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 468.8 bits (1205), Expect = 3.8e-132
Identity = 240/333 (72.07%), Postives = 285/333 (85.59%), Query Frame = 0 

Query: 1   MLLSLNYLSGSPGPSGFGSKSTAEHVTSSASPFLLPSFTAIITGATSGIGAETARILAKR 60
           M+ +  YL G+ G SGFGSKSTAE VT +     L S TA+ITGATSGIGAETAR+LAKR
Sbjct: 1   MIETGKYLLGAAGASGFGSKSTAEEVTENCD---LRSITAVITGATSGIGAETARVLAKR 60

Query: 61  GARLILPSRNLKAAEETKARIESEYSDSRIIVMSLDLSSLSSVRTFVAQFESLNLPLNLL 120
           GARLI P+RN+KAAEE K RI SE+ ++ I+VM LDLSS++SVR FVA FESL+LPLNLL
Sbjct: 61  GARLIFPARNVKAAEEAKERIVSEFPETEIVVMKLDLSSIASVRNFVADFESLDLPLNLL 120

Query: 121 INNAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSI 180
           INNAGK +HEHAISEDGIEMTFATNYLGHFLLT LL+NKM +TA+ TGV+GRIVNV+S I
Sbjct: 121 INNAGKLAHEHAISEDGIEMTFATNYLGHFLLTNLLLNKMIQTAEETGVQGRIVNVTSGI 180

Query: 181 HGWFSGDIIKYLGQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMGANVTVNCVHP 240
           HGWFSGD+I+YL  IS+ K  +DATRAYALSKLANVLHT EL+ RL+++GANVTVNCVHP
Sbjct: 181 HGWFSGDLIEYLRLISQPKCQFDATRAYALSKLANVLHTKELSSRLQKIGANVTVNCVHP 240

Query: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVAMNPRLSHVTGKYFADCNES 300
           G+VRTRLTRDREG +TDLVFF+ASKL+KT+PQAAATTCYVA NPRL +V+GKYF DCNE+
Sbjct: 241 GVVRTRLTRDREGLLTDLVFFLASKLVKTVPQAAATTCYVATNPRLVNVSGKYFTDCNET 300

Query: 301 SPSKLASSPAEAARLWSASEIMVNANSKLVFDP 334
           +PS L ++ +EA +LW+ASEI+V  +SK  FDP
Sbjct: 301 TPSGLGTNSSEATKLWAASEILVTQHSKTSFDP 330

[Close]
BLAST of Lsi09G001720 vs. TAIR 10
Match: AT1G64590.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 455.3 bits (1170), Expect = 4.3e-128
Identity = 232/324 (71.60%), Postives = 278/324 (85.80%), Query Frame = 0 

Query: 1   MLLSLNYLSGSPGPSGFGSKSTAEHVTSSASPFLLPSFTAIITGATSGIGAETARILAKR 60
           M+ ++ +L GS GPSGFGS+STA+HVT ++    L S TAIITGATSGIGAETAR+LAKR
Sbjct: 1   MIETVKHLIGSGGPSGFGSRSTADHVTCNSD---LRSLTAIITGATSGIGAETARVLAKR 60

Query: 61  GARLILPSRNLKAAEETKARIESEYSDSRIIVMSLDLSSLSSVRTFVAQFESLNLPLNLL 120
           GARL+LP+R++K AEETKARI SE+ D+ IIVM LDLSSL+SVR FV  FESLNLPLN+L
Sbjct: 61  GARLVLPARSVKTAEETKARILSEFPDAEIIVMHLDLSSLTSVRRFVDDFESLNLPLNIL 120

Query: 121 INNAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSI 180
           INNAGK++H+HA+SEDG+EMTFATNYLGHFLLTKLL+ KM ETA  TGV+GRIVNV+S +
Sbjct: 121 INNAGKYAHKHALSEDGVEMTFATNYLGHFLLTKLLLKKMIETAAQTGVQGRIVNVTSVV 180

Query: 181 HGWFSGDIIKYLGQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMGANVTVNCVHP 240
           H WFSGD+++YL  ISRN RNYDATRAYALSKLANVLHT EL+R L +M ANVT NCVHP
Sbjct: 181 HSWFSGDMLQYLADISRNNRNYDATRAYALSKLANVLHTVELSRLLHKMDANVTANCVHP 240

Query: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVAMNPRLSHVTGKYFADCNES 300
           GIV+TRLTRDREG +TDLVFF+ SKLLK++PQAAATTCYVA +PRL +V GKYF+DCNE+
Sbjct: 241 GIVKTRLTRDREGVVTDLVFFLTSKLLKSVPQAAATTCYVATSPRLRNVCGKYFSDCNEA 300

Query: 301 SPSKLASSPAEAARLWSASEIMVN 325
             SK  S   +A RLW+AS+++V+
Sbjct: 301 RSSKSGSCNLKAQRLWTASDLLVS 321

[Close]
BLAST of Lsi09G001720 vs. TAIR 10
Match: AT5G50130.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 313.2 bits (801), Expect = 2.6e-85
Identity = 174/324 (53.70%), Postives = 228/324 (70.37%), Query Frame = 0 

Query: 4   SLNYLSGSPGPSGFGSKSTAEHVTSSASPFLLPSFTAIITGATSGIGAETARILAKRGAR 63
           +L YL+G  GP+GFGS+STAE VT   S F     TAIITG TSGIGAETAR+LAKRG R
Sbjct: 4   TLRYLAGIAGPNGFGSRSTAEQVTQH-SFFPCSHLTAIITGGTSGIGAETARVLAKRGVR 63

Query: 64  LILPSRNLKAAEETKARIESEYSDSRIIVMSLDLSSLSSVRTFVAQFESLNLPLNLLINN 123
           +++  R++K AE  K RI  E  ++ II+  +DLSSLSSV  F +QF S +LPLN+LINN
Sbjct: 64  VVMAVRDMKKAEMVKERIIRENPEADIILFEIDLSSLSSVARFCSQFLSQDLPLNILINN 123

Query: 124 AGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSIHGW 183
           AG FS     SE+ IE+TFATN+LGH+LLT++L+ KM +TA+ +G+EGRI+N+SS IH W
Sbjct: 124 AGVFSPNLEFSEEKIELTFATNFLGHYLLTEMLIEKMIDTAEKSGIEGRIINLSSVIHNW 183

Query: 184 FSGDII---KYLGQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMGANVTVNCVHP 243
              D     K L  ISR    Y+ TRAYA SKLA +LH   L+++LK   ANVT+N VHP
Sbjct: 184 VKPDCFSFPKLLHPISR----YNGTRAYAQSKLATILHAKALSKQLKDRNANVTINAVHP 243

Query: 244 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVAMNPRLSHVTGKYFADCNES 303
           GIV+T + R  +G  TD +F +ASKLLK+I Q AATTCYVA++     ++GKYFADCNE+
Sbjct: 244 GIVKTGIIRAHKGLFTDSLFLIASKLLKSISQGAATTCYVALSNETKGLSGKYFADCNET 303

Query: 304 SPSKLASSPAEAARLWSASEIMVN 325
           + S LA+    A +L + S  +++
Sbjct: 304 NCSDLANDEYVALKLCTQSRALIH 322

[Close]
BLAST of Lsi09G001720 vs. TAIR 10
Match: AT5G02540.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 290.0 bits (741), Expect = 2.4e-78
Identity = 161/321 (50.16%), Postives = 219/321 (68.22%), Query Frame = 0 

Query: 8   LSGSPGPSGFGSKSTAEHVTSSASPFLLPSFTAIITGATSGIGAETARILAKRGARLILP 67
           ++G  GPSGFGS STAE VT         + TAIITG T GIG ETAR+L+KRGA +++ 
Sbjct: 7   ITGRRGPSGFGSASTAEEVTQGID---ATNLTAIITGGTGGIGMETARVLSKRGAHVVIG 66

Query: 68  SRNLKAAEETKARIESEYSDSRIIVMSLDLSSLSSVRTFVAQFESLNLPLNLLINNAGKF 127
           +RN+ AAE  K  I  + +++R+ ++ LDLSS+ S++ FV +F +L+LPLNLLINNAG  
Sbjct: 67  ARNMGAAENAKTEILRQNANARVTLLQLDLSSIKSIKAFVREFHALHLPLNLLINNAGVM 126

Query: 128 SHEHAISEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSIHGWFSGD 187
              + +SEDGIE+ FATN++GHFLLT LL++ M  TAK +GVEGRI+NVSS  H +   +
Sbjct: 127 FCPYQLSEDGIELQFATNHIGHFLLTNLLLDTMKNTAKTSGVEGRILNVSSVAHIYTYQE 186

Query: 188 IIKYLGQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMGANVTVNCVHPGIVRTRL 247
            I++      +  +Y   RAY  SKLAN+LH +EL+R+L++ G N+T N VHPG++ T L
Sbjct: 187 GIQF--DSINDICSYSDKRAYGQSKLANILHANELSRQLQEEGVNITANSVHPGLILTNL 246

Query: 248 TRDREGFITDLVFFMASKLLKTIPQAAATTCYVAMNPRLSHVTGKYFADCNESSPSKLAS 307
            +     +  L FF +  L K IPQ AATTCYVA++P +  VTGKYFADCNE +PSKLA 
Sbjct: 247 FQHTALLMRFLKFF-SFYLWKNIPQGAATTCYVALHPSVKGVTGKYFADCNEVTPSKLAR 306

Query: 308 SPAEAARLWSASEIMVNANSK 329
               A +LW  S  ++N+ SK
Sbjct: 307 DETLAQKLWDFSVKLINSVSK 321

[Close]
BLAST of Lsi09G001720 vs. TAIR 10
Match: AT2G37540.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 280.4 bits (716), Expect = 1.9e-75
Identity = 160/321 (49.84%), Postives = 215/321 (66.98%), Query Frame = 0 

Query: 8   LSGSPGPSGFGSKSTAEHVTSSASPFLLPSFTAIITGATSGIGAETARILAKRGARLILP 67
           ++G  G SGFGS STAE VT +     L   TAIITG TSGIG E AR+LA RGA +I+ 
Sbjct: 7   MTGKKGKSGFGSASTAEDVTQAIDASHL---TAIITGGTSGIGLEAARVLAMRGAHVIIA 66

Query: 68  SRNLKAAEETKARIESEYSDSRIIVMSLDLSSLSSVRTFVAQFESLNLPLNLLINNAGKF 127
           +RN KAA E+K  I     ++R+  + +D+SS+ SVR+FV QF +LN+PLN+LINNAG  
Sbjct: 67  ARNPKAANESKEMILQMNPNARVDYLQIDVSSIKSVRSFVDQFLALNVPLNILINNAGVM 126

Query: 128 SHEHAISEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSIHGWFSGD 187
                ++EDGIE  FATN++GHFLLT LL++KM  TA+ +GV+GRIVN+SS  H +   +
Sbjct: 127 FCPFKLTEDGIESQFATNHIGHFLLTNLLLDKMKSTARESGVQGRIVNLSSIAHTYTYSE 186

Query: 188 IIKYLGQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMGANVTVNCVHPGIVRTRL 247
            IK+ G    +   Y   RAY  SKL+N+LH++ L+RRL++ G N+T+N VHPG+V T L
Sbjct: 187 GIKFQG--INDPAGYSERRAYGQSKLSNLLHSNALSRRLQEEGVNITINSVHPGLVTTNL 246

Query: 248 TRDREGFITDLVFFMASKLLKTIPQAAATTCYVAMNPRLSHVTGKYFADCNESSPSKLAS 307
            R   GF   +   M     K IPQ AATTCYVA++P L  VTGKYF DCN  +PSK A+
Sbjct: 247 FR-YSGFSMKVFRAMTFLFWKNIPQGAATTCYVALHPDLEGVTGKYFGDCNIVAPSKFAT 306

Query: 308 SPAEAARLWSASEIMVNANSK 329
           + + A +LW  S  ++++ SK
Sbjct: 307 NNSLADKLWDFSVFLIDSISK 321

The following BLAST results are available for this feature:
BLAST of Lsi09G001720 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
A0A078IS661.1e-7249.38Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=T... [more]
A0A078ISJ67.3e-7248.75Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=T... [more]
A2RVM04.6e-6644.03Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 ... [more]
Q6RVV42.4e-5942.77Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC3... [more]
P598371.9e-4340.64Retinol dehydrogenase 12 OS=Bos taurus OX=9913 GN=RDH12 PE=2 SV=1[more]
BLAST of Lsi09G001720 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5D3C4622.5e-16893.20Short-chain dehydrogenase TIC 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A0A0KKE21.6e-16792.92Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G149870 PE=3 SV=1[more]
A0A6J1I2S61.9e-16089.05short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 OS=Cucurbita max... [more]
A0A6J1HY345.6e-16089.09short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita max... [more]
A0A6J1EMX31.6e-15988.17short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 OS=Cucurbita mos... [more]
BLAST of Lsi09G001720 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038875366.18.9e-17697.34short-chain dehydrogenase TIC 32 A, chloroplastic-like isoform X2 [Benincasa his... [more]
XP_038875365.12.2e-17497.05short-chain dehydrogenase TIC 32 A, chloroplastic-like isoform X1 [Benincasa his... [more]
XP_004143954.11.4e-16893.20short-chain dehydrogenase TIC 32 A, chloroplastic isoform X2 [Cucumis sativus][more]
TYK06084.15.2e-16893.20short-chain dehydrogenase TIC 32 [Cucumis melo var. makuwa][more]
XP_011654698.13.4e-16792.92short-chain dehydrogenase TIC 32 A, chloroplastic isoform X1 [Cucumis sativus] >... [more]
BLAST of Lsi09G001720 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G24050.13.8e-13272.07NAD(P)-binding Rossmann-fold superfamily protein [more]
AT1G64590.14.3e-12871.60NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G50130.12.6e-8553.70NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G02540.12.4e-7850.16NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G37540.11.9e-7549.84NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 208..227
score: 30.81
coord: 165..181
score: 29.05
coord: 115..126
score: 42.05
coord: 231..248
score: 37.54
coord: 39..56
score: 45.43
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00080SDRFAMILYcoord: 208..227
score: 31.71
coord: 115..126
score: 44.39
coord: 171..179
score: 54.94
IPR002347Short-chain dehydrogenase/reductase SDRPFAMPF00106adh_shortcoord: 39..250
e-value: 1.4E-32
score: 112.8
NoneNo IPR availableGENE3D3.40.50.720coord: 34..328
e-value: 8.5E-76
score: 256.8
NoneNo IPR availablePANTHERPTHR24320:SF210MITOCHONDRIAL PYRUVATE CARRIERcoord: 10..329
NoneNo IPR availablePANTHERPTHR24320RETINOL DEHYDROGENASEcoord: 10..329
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 39..301

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi09G001720.1Lsi09G001720.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016491 oxidoreductase activity