Lsi08G016280 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi08G016280
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Pectate lyase
Location	chr08: 24200656 .. 24203075 (+)
RNA-Seq Expression	Lsi08G016280
Synteny	Lsi08G016280

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTTTCTTCACCATCACCAAACTTTGAATCCTCTGTCTTTGCCTTTCTCTGCTTCTTCACACACAAAAAGAGAAAGAAACAGAGAGCTATGGAAAATCCCATTTTTCTTCTAATAATAACATTCTTCAGTGTTCTTCTCCAAATGTGGTCTGTTTCATCTTCTCCAGTCGCTGATCCTGAAGTAGTTGTCCAAGAAGTTCTAACGTAAGTCCTCTCTTCAAATTCACAGCTCTGCATTTTATTCCAACTTCTTTTCCCTACATGATCTTTTAAATCTCAACTTTCTTGTACCAAGATCACCTACATTTAATGATTTTAGTCAGATGCTTACAACTTTTTTTTATCCCGTTCCTTTGATAATCATTTGGTTTTTCGAAAGTTAAGCTTATAAACGATAGTTCTTCTCAAGTTTTTAAAATTCATGCCAAATTTTGAATGAAATGAAAAAGTAATTTTTGTTTTCGAAATTTGGTTAAGAATTCAAATGTTTCCTTTAGAAAAATGAAGAGCATAATAAAGTAATTGTGAAAAAGAAATAAACATAATTTTTAAAAATCAAATGATCATCAAAATAAATTTAAAATTCTCTCGCGCACATGTAACATGAAAAATCTTTAGTTACATTTTTCCAACATCTCAAATGATTTCTCATATTTGAGATAGATAGTTTTCAAAAATCGAATGATTATTAAATTCAAGCATCTACTTCTAATAATTTCTTCTTGGGAGTTTGATTACAATCCACCCCATTTTGTTAAATATTCTTTTTCTTTTGTGGGTGATGATTTCAGGAGTATCAACAATGCAACAACAAGACGAAATTTGGGATTCCTATCTTGTGGAATTGGGAATCCAATAGACGATTGTTGGCGATGTGATCCCAATTGGGAGAAGAATCGAAAGCGGCTAGCCGACTGTGCCATTGGGTTTGGGAAGCGAGCCATTGGTGGCAAAAACGGAAAATTCTACATTGTCACTGACCCCTCTGACAAGGACGTGGTGAACCCAAAACCAGGCACTCTCCGCCACGCCGTAATCCAAAAGGAACCGTTATGGATTATCTTCAAACGTGACATGGTGATCAAATTAAAAGCAGAGCTTCTCATGAACTCTTTCAAAACCATCGACGGTCGCGGCGTGTCCGTCCACATTGCCGGTGGGCCTTGCATTACCATTCAATTTGTGACCAACATTATAATCCACGGCATCAACATTCACGATTGCAAGCAAGGTGGGAACACGTACGTCAGAGACTCGCCGGAGCATTACGGTTGGAGGACTTTGTCGGACGGCGATGGGGTTTCCATATTTGGGGGCAGTCATATTTGGGTCGACCATTGCTCTTTGTCCAATTGTCGTGATGGGTTGATTGATGCGATTCATGGGTCGACGGCTATCACTCTTTCCAACAATTACATGACCCACCACAACAAAGTTATGCTTTTAGGGCATAGTGATTCCTATACACAAGACAAGAACATGCAAGTCACCATTGCTTTTAACCATTTTGGTGAAGGTCTTGTGCAAAGAATGCCACGGTGAGTCGTCATTTTTGTTTGAAAGGACTTGTTAAGTGTTTAATTAAAAAAAAAAAATTTTTAATAATTAAAAAATCGTCCTCTGAATTAACTTGAAATGTGTGTGATGGACAGATGTCGTCATGGATATTTCCATGTGGTGAATAATGACTATACTCACTGGATAATGTACGCCATTGGTGGAAGTGCATCCCCTACCATCAATAGCCAAGGAAATAGATACCTCGCTCCCAATAACCAAGATAGAAAAGAGGTTAGAAATTCCGTTGTTAATAATCATATAAATTGAAGTTGACGATAGAACGAATTTAAAAAGTCATTTCAAATAGATGGTTTTAAATTTAGAATACTTTATCAATTTAAATTAATGGTTATTTCAGGTGACAAAGCGAGAGGATGCACCACAGAGCAAGTGGAAGAATTGGAATTGGAGATCAGATGGAGATTTGATGTTGAATGGAGCATTCTTCACAAGGTCCGGTGCTGGAGCATCATCTAGCTATGCAAGAGCATCGAGCTTGAGTGCGAAGTCGTCGTCGCTGGTGAGCTCGATCACGGCGGGAGCAGGGGTGTTGCGGTGCCGAAAAGGCTCTAAATGTTAGTCCAACTACCGTCGGCAAGGACCGACGATATATATGGACCAAAATGACAGTATATTGAAGATAATAGCAAGGAGAGATTTGAAAATCTTCTTGTATAGTAATTTTTACTTTGTCTTACTTGATTGGTCACAACTGAAAGGCTTTCTTTTTTTTTTAAGAAAAAGTTGTATTTCAGAGTGAGTGGCCCACCATTTGTAATTCTGTCTAATTACAATATAATATAAATATTACTATGTGAAAAAGAAAAATGTTATTCTCTTTTCAAAAAAAGAAACCCTTC

mRNA sequence

CTTTTCTTCACCATCACCAAACTTTGAATCCTCTGTCTTTGCCTTTCTCTGCTTCTTCACACACAAAAAGAGAAAGAAACAGAGAGCTATGGAAAATCCCATTTTTCTTCTAATAATAACATTCTTCAGTGTTCTTCTCCAAATGTGGTCTGTTTCATCTTCTCCAGTCGCTGATCCTGAAGTAGTTGTCCAAGAAGTTCTAACGAGTATCAACAATGCAACAACAAGACGAAATTTGGGATTCCTATCTTGTGGAATTGGGAATCCAATAGACGATTGTTGGCGATGTGATCCCAATTGGGAGAAGAATCGAAAGCGGCTAGCCGACTGTGCCATTGGGTTTGGGAAGCGAGCCATTGGTGGCAAAAACGGAAAATTCTACATTGTCACTGACCCCTCTGACAAGGACGTGGTGAACCCAAAACCAGGCACTCTCCGCCACGCCGTAATCCAAAAGGAACCGTTATGGATTATCTTCAAACGTGACATGGTGATCAAATTAAAAGCAGAGCTTCTCATGAACTCTTTCAAAACCATCGACGGTCGCGGCGTGTCCGTCCACATTGCCGGTGGGCCTTGCATTACCATTCAATTTGTGACCAACATTATAATCCACGGCATCAACATTCACGATTGCAAGCAAGGTGGGAACACGTACGTCAGAGACTCGCCGGAGCATTACGGTTGGAGGACTTTGTCGGACGGCGATGGGGTTTCCATATTTGGGGGCAGTCATATTTGGGTCGACCATTGCTCTTTGTCCAATTGTCGTGATGGGTTGATTGATGCGATTCATGGGTCGACGGCTATCACTCTTTCCAACAATTACATGACCCACCACAACAAAGTTATGCTTTTAGGGCATAGTGATTCCTATACACAAGACAAGAACATGCAAGTCACCATTGCTTTTAACCATTTTGGTGAAGGTCTTGTGCAAAGAATGCCACGATGTCGTCATGGATATTTCCATGTGGTGAATAATGACTATACTCACTGGATAATGTACGCCATTGGTGGAAGTGCATCCCCTACCATCAATAGCCAAGGAAATAGATACCTCGCTCCCAATAACCAAGATAGAAAAGAGGTGACAAAGCGAGAGGATGCACCACAGAGCAAGTGGAAGAATTGGAATTGGAGATCAGATGGAGATTTGATGTTGAATGGAGCATTCTTCACAAGGTCCGGTGCTGGAGCATCATCTAGCTATGCAAGAGCATCGAGCTTGAGTGCGAAGTCGTCGTCGCTGGTGAGCTCGATCACGGCGGGAGCAGGGGTGTTGCGGTGCCGAAAAGGCTCTAAATGTTAGTCCAACTACCGTCGGCAAGGACCGACGATATATATGGACCAAAATGACAGTATATTGAAGATAATAGCAAGGAGAGATTTGAAAATCTTCTTGTATAGTAATTTTTACTTTGTCTTACTTGATTGGTCACAACTGAAAGGCTTTCTTTTTTTTTTAAGAAAAAGTTGTATTTCAGAGTGAGTGGCCCACCATTTGTAATTCTGTCTAATTACAATATAATATAAATATTACTATGTGAAAAAGAAAAATGTTATTCTCTTTTCAAAAAAAGAAACCCTTC

Coding sequence (CDS)

ATGGAAAATCCCATTTTTCTTCTAATAATAACATTCTTCAGTGTTCTTCTCCAAATGTGGTCTGTTTCATCTTCTCCAGTCGCTGATCCTGAAGTAGTTGTCCAAGAAGTTCTAACGAGTATCAACAATGCAACAACAAGACGAAATTTGGGATTCCTATCTTGTGGAATTGGGAATCCAATAGACGATTGTTGGCGATGTGATCCCAATTGGGAGAAGAATCGAAAGCGGCTAGCCGACTGTGCCATTGGGTTTGGGAAGCGAGCCATTGGTGGCAAAAACGGAAAATTCTACATTGTCACTGACCCCTCTGACAAGGACGTGGTGAACCCAAAACCAGGCACTCTCCGCCACGCCGTAATCCAAAAGGAACCGTTATGGATTATCTTCAAACGTGACATGGTGATCAAATTAAAAGCAGAGCTTCTCATGAACTCTTTCAAAACCATCGACGGTCGCGGCGTGTCCGTCCACATTGCCGGTGGGCCTTGCATTACCATTCAATTTGTGACCAACATTATAATCCACGGCATCAACATTCACGATTGCAAGCAAGGTGGGAACACGTACGTCAGAGACTCGCCGGAGCATTACGGTTGGAGGACTTTGTCGGACGGCGATGGGGTTTCCATATTTGGGGGCAGTCATATTTGGGTCGACCATTGCTCTTTGTCCAATTGTCGTGATGGGTTGATTGATGCGATTCATGGGTCGACGGCTATCACTCTTTCCAACAATTACATGACCCACCACAACAAAGTTATGCTTTTAGGGCATAGTGATTCCTATACACAAGACAAGAACATGCAAGTCACCATTGCTTTTAACCATTTTGGTGAAGGTCTTGTGCAAAGAATGCCACGATGTCGTCATGGATATTTCCATGTGGTGAATAATGACTATACTCACTGGATAATGTACGCCATTGGTGGAAGTGCATCCCCTACCATCAATAGCCAAGGAAATAGATACCTCGCTCCCAATAACCAAGATAGAAAAGAGGTGACAAAGCGAGAGGATGCACCACAGAGCAAGTGGAAGAATTGGAATTGGAGATCAGATGGAGATTTGATGTTGAATGGAGCATTCTTCACAAGGTCCGGTGCTGGAGCATCATCTAGCTATGCAAGAGCATCGAGCTTGAGTGCGAAGTCGTCGTCGCTGGTGAGCTCGATCACGGCGGGAGCAGGGGTGTTGCGGTGCCGAAAAGGCTCTAAATGTTAG

Protein sequence

MENPIFLLIITFFSVLLQMWSVSSSPVADPEVVVQEVLTSINNATTRRNLGFLSCGIGNPIDDCWRCDPNWEKNRKRLADCAIGFGKRAIGGKNGKFYIVTDPSDKDVVNPKPGTLRHAVIQKEPLWIIFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTNIIIHGINIHDCKQGGNTYVRDSPEHYGWRTLSDGDGVSIFGGSHIWVDHCSLSNCRDGLIDAIHGSTAITLSNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWIMYAIGGSASPTINSQGNRYLAPNNQDRKEVTKREDAPQSKWKNWNWRSDGDLMLNGAFFTRSGAGASSSYARASSLSAKSSSLVSSITAGAGVLRCRKGSKC

Homology

[Close]

BLAST of Lsi08G016280 vs. ExPASy Swiss-Prot
Match: Q9FXD8 (Probable pectate lyase 5 OS=Arabidopsis thaliana OX=3702 GN=At1g67750 PE=2 SV=2)

HSP 1 Score: 678.3 bits (1749), Expect = 5.3e-194
Identity = 324/403 (80.40%), Postives = 359/403 (89.08%), Query Frame = 0


Query: 8   LIITFFSVLLQMWS---VSSSPVADPEVVVQEVLTSINNATTRRNLGFLSCGIGNPIDDC 67
           L ++ FS LL + S   ++S+PV++PE+VVQEV   IN   +RRNLG LSCG GNPIDDC
Sbjct: 8   LSLSLFSCLLLVLSPTFIASTPVSEPELVVQEVNEKIN--ASRRNLGVLSCGTGNPIDDC 67

Query: 68  WRCDPNWEKNRKRLADCAIGFGKRAIGGKNGKFYIVTDPSDKDVVNPKPGTLRHAVIQKE 127
           WRCDP WEKNR+RLADCAIGFGK AIGG++GK Y+VTD SDKDVVNPKPGTLRHAVIQ E
Sbjct: 68  WRCDPKWEKNRQRLADCAIGFGKHAIGGRDGKIYVVTDSSDKDVVNPKPGTLRHAVIQDE 127

Query: 128 PLWIIFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTNIIIHGINIHDCK 187
           PLWIIF RDMVIKLK EL+MNSFKTIDGRG SVHIAGG CIT+Q+VTNIIIHG+NIHDCK
Sbjct: 128 PLWIIFARDMVIKLKEELIMNSFKTIDGRGASVHIAGGACITVQYVTNIIIHGVNIHDCK 187

Query: 188 QGGNTYVRDSPEHYGWRTLSDGDGVSIFGGSHIWVDHCSLSNCRDGLIDAIHGSTAITLS 247
           + GN YVRDSP HYGWRT SDGD VSIFGGSH+WVDHCSLSNC DGLIDAIHGSTAIT+S
Sbjct: 188 RKGNAYVRDSPSHYGWRTASDGDAVSIFGGSHVWVDHCSLSNCADGLIDAIHGSTAITIS 247

Query: 248 NNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW 307
           NNY++HHNKVMLLGHSDSYT+DKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW
Sbjct: 248 NNYLSHHNKVMLLGHSDSYTRDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW 307

Query: 308 IMYAIGGSASPTINSQGNRYLAPNNQDRKEVTKREDAPQSKWKNWNWRSDGDLMLNGAFF 367
            MYAIGGSA+PTINSQGNR+LAPN+   KEVTK EDAP+SKWK WNWRS+GDL LNGAFF
Sbjct: 308 QMYAIGGSAAPTINSQGNRFLAPNDHVFKEVTKYEDAPRSKWKKWNWRSEGDLFLNGAFF 367

Query: 368 TRSGAGASSSYARASSLSAKSSSLVSSITAGAGVLRCRKGSKC 408
           T SG GASSSYA+ASSLSA+ SSLV+S+T+ AG L CRKGS+C
Sbjct: 368 TPSGGGASSSYAKASSLSARPSSLVASVTSNAGALFCRKGSRC 408

[Close]

BLAST of Lsi08G016280 vs. ExPASy Swiss-Prot
Match: Q93Z25 (Probable pectate lyase 22 OS=Arabidopsis thaliana OX=3702 GN=At5g63180 PE=2 SV=1)

HSP 1 Score: 655.6 bits (1690), Expect = 3.7e-187
Identity = 306/400 (76.50%), Postives = 350/400 (87.50%), Query Frame = 0


Query: 10  ITFFSVLLQMWSVSSSPVADPEVVVQEVLTSINNATTRRNLGFLSCGIGNPIDDCWRCDP 69
           + FF  +L     SS PV+DPE+VV+EV   IN + +RR LGF SCG GNPIDDCWRCD 
Sbjct: 33  LIFFCCILFSALASSLPVSDPELVVEEVHRKINESISRRKLGFFSCGSGNPIDDCWRCDK 92

Query: 70  NWEKNRKRLADCAIGFGKRAIGGKNGKFYIVTDPSDKDVVNPKPGTLRHAVIQKEPLWII 129
           +WEKNRKRLADC IGFGK AIGG++G+ Y+VTDP + D VNP+PGTLR+AVIQ EPLWII
Sbjct: 93  DWEKNRKRLADCGIGFGKNAIGGRDGEIYVVTDPGNDDPVNPRPGTLRYAVIQDEPLWII 152

Query: 130 FKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTNIIIHGINIHDCKQGGNT 189
           FKRDM I+LK EL+MNSFKT+DGRG SVHI+GGPCITIQ+VTNIIIHG++IHDCKQGGNT
Sbjct: 153 FKRDMTIQLKEELIMNSFKTLDGRGASVHISGGPCITIQYVTNIIIHGLHIHDCKQGGNT 212

Query: 190 YVRDSPEHYGWRTLSDGDGVSIFGGSHIWVDHCSLSNCRDGLIDAIHGSTAITLSNNYMT 249
           YVRDSPEHYG+RT+SDGDGVSIFGGSH+WVDHCSLSNC DGLIDAI GSTAIT+SNNY+T
Sbjct: 213 YVRDSPEHYGYRTVSDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIRGSTAITISNNYLT 272

Query: 250 HHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWIMYAI 309
           HHNKVMLLGHSD+Y QDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW MYAI
Sbjct: 273 HHNKVMLLGHSDTYEQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAI 332

Query: 310 GGSASPTINSQGNRYLAPNNQDRKEVTKREDAPQSKWKNWNWRSDGDLMLNGAFFTRSGA 369
           GGSA+PTINSQGNR+LAP++   KEVTK EDAP+ +W+NWNWRS+GDL+LNGAFFT SGA
Sbjct: 333 GGSANPTINSQGNRFLAPDDSSSKEVTKHEDAPEDEWRNWNWRSEGDLLLNGAFFTYSGA 392

Query: 370 G--ASSSYARASSLSAKSSSLVSSITAGAGVLRCRKGSKC 408
           G   SSSY++ASSL+A+ SS V  IT  +G L C++GS C
Sbjct: 393 GPAKSSSYSKASSLAARPSSHVGEITIASGALSCKRGSHC 432

[Close]

BLAST of Lsi08G016280 vs. ExPASy Swiss-Prot
Match: Q9C5M8 (Probable pectate lyase 18 OS=Arabidopsis thaliana OX=3702 GN=At4g24780 PE=2 SV=2)

HSP 1 Score: 642.9 bits (1657), Expect = 2.5e-183
Identity = 306/401 (76.31%), Postives = 346/401 (86.28%), Query Frame = 0


Query: 8   LIITFFSVLLQMWSVSSSPVADPEVVVQEVLTSIN-NATTRRNLGFLSCGIGNPIDDCWR 67
           L IT  S LL      SSPV DPE VV+EV  SIN +   RR LG+LSC  GNPIDDCWR
Sbjct: 8   LFITIVSFLLYAPLFLSSPVPDPESVVEEVHKSINASVAGRRKLGYLSCTTGNPIDDCWR 67

Query: 68  CDPNWEKNRKRLADCAIGFGKRAIGGKNGKFYIVTDPSDKDVVNPKPGTLRHAVIQKEPL 127
           CDP+WE++R+RLADCAIGFGK AIGG++G+ Y+VTD  + + V+PKPGTLRHAV+Q EPL
Sbjct: 68  CDPHWEQHRQRLADCAIGFGKNAIGGRDGRIYVVTDSGNDNPVSPKPGTLRHAVVQDEPL 127

Query: 128 WIIFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTNIIIHGINIHDCKQG 187
           WIIF+RDM I+LK EL+MNSFKTIDGRG SVHI+GGPCITIQ+VTNIIIHGI+IHDCKQG
Sbjct: 128 WIIFQRDMTIQLKEELIMNSFKTIDGRGASVHISGGPCITIQYVTNIIIHGIHIHDCKQG 187

Query: 188 GNTYVRDSPEHYGWRTLSDGDGVSIFGGSHIWVDHCSLSNCRDGLIDAIHGSTAITLSNN 247
           GN  VR SP H+GWRT+SDGDGVSIFGGSH+WVDHCS SNC DGLIDAI GSTAITLSNN
Sbjct: 188 GNAMVRSSPRHFGWRTISDGDGVSIFGGSHVWVDHCSFSNCEDGLIDAIMGSTAITLSNN 247

Query: 248 YMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWIM 307
           +MTHH+KVMLLGHSD+Y++DKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW M
Sbjct: 248 HMTHHDKVMLLGHSDTYSRDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEM 307

Query: 308 YAIGGSASPTINSQGNRYLAPNNQDRKEVTKREDAPQSKWKNWNWRSDGDLMLNGAFFTR 367
           YAIGGSA+PTINSQGNR+LAPN +  KEVTK EDAP+S+WK WNWRS GDL+LNGAFFT 
Sbjct: 308 YAIGGSANPTINSQGNRFLAPNIRFSKEVTKHEDAPESEWKRWNWRSSGDLLLNGAFFTP 367

Query: 368 SGAGASSSYARASSLSAKSSSLVSSITAGAGVLRCRKGSKC 408
           SG  ASSSYA+ASSL AK SSLV  +T+ +G L CRKGS+C
Sbjct: 368 SGGAASSSYAKASSLGAKPSSLVGPLTSTSGALNCRKGSRC 408

[Close]

BLAST of Lsi08G016280 vs. ExPASy Swiss-Prot
Match: O24554 (Pectate lyase OS=Zinnia violacea OX=34245 PE=1 SV=1)

HSP 1 Score: 615.9 bits (1587), Expect = 3.2e-175
Identity = 297/401 (74.06%), Postives = 335/401 (83.54%), Query Frame = 0


Query: 7   LLIITFFSVLLQMWSVSSSPVADPEVVVQEVLTSINNATTRRNLGFLSCGIGNPIDDCWR 66
           L +I F S L      SSSP   P  +V EV  SIN   +RRNLG+LSCG GNPIDDCWR
Sbjct: 6   LPLILFISSLA---IASSSPSRTPHAIVNEVHKSIN--ASRRNLGYLSCGTGNPIDDCWR 65

Query: 67  CDPNWEKNRKRLADCAIGFGKRAIGGKNGKFYIVTDPSDKDVVNPKPGTLRHAVIQKEPL 126
           CDPNW  NR+RLADCAIGFGK A+GG+NG+ Y+VTDP + D VNP PGTLR+AVIQ EPL
Sbjct: 66  CDPNWANNRQRLADCAIGFGKNAMGGRNGRIYVVTDPGNDDPVNPVPGTLRYAVIQDEPL 125

Query: 127 WIIFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTNIIIHGINIHDCKQG 186
           WIIFKRDMVI+L+ EL+MNS KTIDGRGV+VHI  GPCITI + +NIIIHGI+IHDCKQ 
Sbjct: 126 WIIFKRDMVIQLRQELVMNSHKTIDGRGVNVHIGNGPCITIHYASNIIIHGIHIHDCKQA 185

Query: 187 GNTYVRDSPEHYGWRTLSDGDGVSIFGGSHIWVDHCSLSNCRDGLIDAIHGSTAITLSNN 246
           GN  +R+SP H GW T SDGDG+SIF    IW+DH SLSNC DGLIDAIHGSTAIT+SNN
Sbjct: 186 GNGNIRNSPHHSGWWTQSDGDGISIFASKDIWIDHNSLSNCHDGLIDAIHGSTAITISNN 245

Query: 247 YMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWIM 306
           YMTHH+KVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW M
Sbjct: 246 YMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEM 305

Query: 307 YAIGGSASPTINSQGNRYLAPNNQDRKEVTKREDAPQSKWKNWNWRSDGDLMLNGAFFTR 366
           YAIGGSASPTI SQGNR+LAPN +  KEVTK E+AP+S+WKNWNWRS+GDLMLNGA+F  
Sbjct: 306 YAIGGSASPTIYSQGNRFLAPNTRFDKEVTKHENAPESEWKNWNWRSEGDLMLNGAYFRE 365

Query: 367 SGAGASSSYARASSLSAKSSSLVSSITAGAGVLRCRKGSKC 408
           SG  A+SS+ARASSLS + S+LV+S+T  AG L CRKGS+C
Sbjct: 366 SGGRAASSFARASSLSGRPSTLVASMTRSAGALVCRKGSRC 401

[Close]

BLAST of Lsi08G016280 vs. ExPASy Swiss-Prot
Match: Q9LTZ0 (Putative pectate lyase 11 OS=Arabidopsis thaliana OX=3702 GN=At3g27400 PE=3 SV=2)

HSP 1 Score: 610.1 bits (1572), Expect = 1.8e-173
Identity = 290/402 (72.14%), Postives = 335/402 (83.33%), Query Frame = 0


Query: 6   FLLIITFFSVLLQMWSVSSSPVADPEVVVQEVLTSINNATTRRNLGFLSCGIGNPIDDCW 65
           FLL++T  + L    S+    V DP +VV +V  S+ NA +RR+L +LSC  GNPIDDCW
Sbjct: 13  FLLLLTIGNTLAFSSSLPDH-VQDPNLVVDDVNRSVFNA-SRRSLAYLSCRTGNPIDDCW 72

Query: 66  RCDPNWEKNRKRLADCAIGFGKRAIGGKNGKFYIVTDPSDKDVVNPKPGTLRHAVIQKEP 125
           RCDPNWE NR+RLADCAIGFGK AIGG+ G+ Y+VTDP++ D VNP+PGTLR+AV Q+EP
Sbjct: 73  RCDPNWETNRQRLADCAIGFGKNAIGGRKGRIYVVTDPANDDPVNPRPGTLRYAVTQEEP 132

Query: 126 LWIIFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTNIIIHGINIHDCKQ 185
           LWIIFKRDMVI+LK EL++ SFKTIDGRG SVHI  GPC+ I + TNIIIHGINIHDCK 
Sbjct: 133 LWIIFKRDMVIRLKKELIITSFKTIDGRGSSVHITDGPCLKIHYATNIIIHGINIHDCKP 192

Query: 186 GGNTYVRDSPEHYGWRTLSDGDGVSIFGGSHIWVDHCSLSNCRDGLIDAIHGSTAITLSN 245
           G    ++D P H GW   SDGD V+IFGG H+W+DHCSLSNC DGLIDAIHGSTAIT+SN
Sbjct: 193 GSGGMIKDGPHHTGWWMQSDGDAVAIFGGKHVWIDHCSLSNCDDGLIDAIHGSTAITISN 252

Query: 246 NYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWI 305
           N+MTHH+KVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW 
Sbjct: 253 NHMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWE 312

Query: 306 MYAIGGSASPTINSQGNRYLAPNNQDRKEVTKREDAPQSKWKNWNWRSDGDLMLNGAFFT 365
           MYAIGGSASPTI SQGNR+LAPN +  KEVTK EDAP+SKW++WNWRS+GD++LNGA+F 
Sbjct: 313 MYAIGGSASPTIYSQGNRFLAPNTRFNKEVTKHEDAPESKWRDWNWRSEGDMLLNGAYFR 372

Query: 366 RSGAGASSSYARASSLSAKSSSLVSSITAGAGVLRCRKGSKC 408
            SGA A S+YARASSLSA+ SSLV SIT  AG L CR+G +C
Sbjct: 373 ESGAEAPSTYARASSLSARPSSLVGSITTTAGTLSCRRGRRC 412

[Close]

BLAST of Lsi08G016280 vs. ExPASy TrEMBL
Match: A0A6J1K9C7 (Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111491836 PE=3 SV=1)

HSP 1 Score: 797.0 bits (2057), Expect = 3.8e-227
Identity = 383/407 (94.10%), Postives = 398/407 (97.79%), Query Frame = 0


Query: 1   MENPIFLLIITFFSVLLQMWSVSSSPVADPEVVVQEVLTSINNATTRRNLGFLSCGIGNP 60
           ME P FL IIT F++LLQM S+SSSPVADPE+VVQEVL+SINNATTRRNLGFLSCG GNP
Sbjct: 1   MEIPSFLPIIT-FTLLLQMCSISSSPVADPELVVQEVLSSINNATTRRNLGFLSCGTGNP 60

Query: 61  IDDCWRCDPNWEKNRKRLADCAIGFGKRAIGGKNGKFYIVTDPSDKDVVNPKPGTLRHAV 120
           IDDCWRCDPNWEKNRKRLADC IGFGKRAIGGKNGKFY+VTDPSDKDVVNPKPGTLRHAV
Sbjct: 61  IDDCWRCDPNWEKNRKRLADCGIGFGKRAIGGKNGKFYVVTDPSDKDVVNPKPGTLRHAV 120

Query: 121 IQKEPLWIIFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTNIIIHGINI 180
           IQKEPLWI FKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQ+VTNIIIHGINI
Sbjct: 121 IQKEPLWITFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQYVTNIIIHGINI 180

Query: 181 HDCKQGGNTYVRDSPEHYGWRTLSDGDGVSIFGGSHIWVDHCSLSNCRDGLIDAIHGSTA 240
           HDCKQGGNTYVRDSPEHYGWRTLSDGDGVSIFGGSH+W+DHCSLSNCRDGLIDAIHGSTA
Sbjct: 181 HDCKQGGNTYVRDSPEHYGWRTLSDGDGVSIFGGSHVWIDHCSLSNCRDGLIDAIHGSTA 240

Query: 241 ITLSNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNND 300
           IT+SNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNND
Sbjct: 241 ITVSNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNND 300

Query: 301 YTHWIMYAIGGSASPTINSQGNRYLAPNNQDRKEVTKREDAPQSKWKNWNWRSDGDLMLN 360
           YTHWIMYAIGGSASPTINSQGNRYLAPNN+DRKEVTKREDAPQSKWKNWNWRS GDLMLN
Sbjct: 301 YTHWIMYAIGGSASPTINSQGNRYLAPNNRDRKEVTKREDAPQSKWKNWNWRSSGDLMLN 360

Query: 361 GAFFTRSGAGASSSYARASSLSAKSSSLVSSITAGAGVLRCRKGSKC 408
           GAFFTRSGAG+SSSYARASSLSAKSSSLVS++TAGAGVLRCRKGS+C
Sbjct: 361 GAFFTRSGAGSSSSYARASSLSAKSSSLVSTMTAGAGVLRCRKGSRC 406

[Close]

BLAST of Lsi08G016280 vs. ExPASy TrEMBL
Match: A0A6J1HD44 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111462874 PE=3 SV=1)

HSP 1 Score: 794.7 bits (2051), Expect = 1.9e-226
Identity = 381/407 (93.61%), Postives = 398/407 (97.79%), Query Frame = 0


Query: 1   MENPIFLLIITFFSVLLQMWSVSSSPVADPEVVVQEVLTSINNATTRRNLGFLSCGIGNP 60
           ME P FL IIT F++LLQM ++SSSPVADP++VVQEVL+SINNATTRRNLGFLSCG GNP
Sbjct: 1   MEIPSFLPIIT-FTLLLQMCAISSSPVADPQLVVQEVLSSINNATTRRNLGFLSCGTGNP 60

Query: 61  IDDCWRCDPNWEKNRKRLADCAIGFGKRAIGGKNGKFYIVTDPSDKDVVNPKPGTLRHAV 120
           IDDCWRCDPNWEKNRKRLADC IGFGKRAIGGKNGKFY+VTDPSDKDVVNPKPGTLRHAV
Sbjct: 61  IDDCWRCDPNWEKNRKRLADCGIGFGKRAIGGKNGKFYVVTDPSDKDVVNPKPGTLRHAV 120

Query: 121 IQKEPLWIIFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTNIIIHGINI 180
           IQKEPLWI FKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQ+VTNIIIHGINI
Sbjct: 121 IQKEPLWITFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQYVTNIIIHGINI 180

Query: 181 HDCKQGGNTYVRDSPEHYGWRTLSDGDGVSIFGGSHIWVDHCSLSNCRDGLIDAIHGSTA 240
           HDCKQGGNTYVRDSPEHYGWRTLSDGDGVSIFGGSH+W+DHCSLSNCRDGLIDAIHGSTA
Sbjct: 181 HDCKQGGNTYVRDSPEHYGWRTLSDGDGVSIFGGSHVWIDHCSLSNCRDGLIDAIHGSTA 240

Query: 241 ITLSNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNND 300
           IT+SNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNND
Sbjct: 241 ITVSNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNND 300

Query: 301 YTHWIMYAIGGSASPTINSQGNRYLAPNNQDRKEVTKREDAPQSKWKNWNWRSDGDLMLN 360
           YTHWIMYAIGGSASPTINSQGNRYLAPNN+DRKEVTKREDAPQSKWKNWNWRS GDLMLN
Sbjct: 301 YTHWIMYAIGGSASPTINSQGNRYLAPNNRDRKEVTKREDAPQSKWKNWNWRSSGDLMLN 360

Query: 361 GAFFTRSGAGASSSYARASSLSAKSSSLVSSITAGAGVLRCRKGSKC 408
           GAFFTRSGAG+SSSYARASSLSAKSSSLVS++TAGAGVLRCRKGS+C
Sbjct: 361 GAFFTRSGAGSSSSYARASSLSAKSSSLVSTMTAGAGVLRCRKGSRC 406

[Close]

BLAST of Lsi08G016280 vs. ExPASy TrEMBL
Match: A0A1S3BB18 (Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103487738 PE=3 SV=1)

HSP 1 Score: 778.5 bits (2009), Expect = 1.4e-221
Identity = 376/415 (90.60%), Postives = 394/415 (94.94%), Query Frame = 0


Query: 1   MENPIFLLI-------ITFFSVLLQMWSV-SSSPVADPEVVVQEVLTSINNATTRRNLGF 60
           MENP F++I       + F  V+LQM S+ SSSPV DP++V QEVLTSINNATTRRNLGF
Sbjct: 1   MENPKFVVIMIISIKVLVFSMVMLQMCSISSSSPVPDPDLVAQEVLTSINNATTRRNLGF 60

Query: 61  LSCGIGNPIDDCWRCDPNWEKNRKRLADCAIGFGKRAIGGKNGKFYIVTDPSDKDVVNPK 120
           LSC  GNPIDDCWRCD NWEKNRKRLA+CAIGFGKRAIGGKNG++YIVTDPSD DVVNPK
Sbjct: 61  LSCRTGNPIDDCWRCDANWEKNRKRLANCAIGFGKRAIGGKNGRYYIVTDPSDNDVVNPK 120

Query: 121 PGTLRHAVIQKEPLWIIFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTN 180
           PGTLRHAVIQKEPLWIIFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTN
Sbjct: 121 PGTLRHAVIQKEPLWIIFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTN 180

Query: 181 IIIHGINIHDCKQGGNTYVRDSPEHYGWRTLSDGDGVSIFGGSHIWVDHCSLSNCRDGLI 240
           IIIHGINIHDCKQGGNTYVRDSPEHYGWRT+SDGDG+SIFGGSH+W+DHCSLSNCRDGLI
Sbjct: 181 IIIHGINIHDCKQGGNTYVRDSPEHYGWRTVSDGDGISIFGGSHVWIDHCSLSNCRDGLI 240

Query: 241 DAIHGSTAITLSNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHG 300
           DAIHGSTAIT+SNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHG
Sbjct: 241 DAIHGSTAITVSNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHG 300

Query: 301 YFHVVNNDYTHWIMYAIGGSASPTINSQGNRYLAPNNQDRKEVTKREDAPQSKWKNWNWR 360
           YFHVVNNDYTHWIMYAIGGSASPTINSQGNRYLAPNNQDRKEVTKREDAPQSKWKNWNWR
Sbjct: 301 YFHVVNNDYTHWIMYAIGGSASPTINSQGNRYLAPNNQDRKEVTKREDAPQSKWKNWNWR 360

Query: 361 SDGDLMLNGAFFTRSGAGASSSYARASSLSAKSSSLVSSITAGAGVLRCRKGSKC 408
           SDGDLMLNGAFF RSGAGASS YARASSLSAKSSSLVSSIT+GAGVL+CRKGS+C
Sbjct: 361 SDGDLMLNGAFFVRSGAGASSRYARASSLSAKSSSLVSSITSGAGVLKCRKGSRC 415

[Close]

BLAST of Lsi08G016280 vs. ExPASy TrEMBL
Match: A0A6J1BR07 (Pectate lyase OS=Momordica charantia OX=3673 GN=LOC111004807 PE=3 SV=1)

HSP 1 Score: 773.5 bits (1996), Expect = 4.5e-220
Identity = 370/405 (91.36%), Postives = 389/405 (96.05%), Query Frame = 0


Query: 3   NPIFLLIITFFSVLLQMWSVSSSPVADPEVVVQEVLTSINNATTRRNLGFLSCGIGNPID 62
           NPIF+L+I F ++LLQM S+SSSPVADPE +V+EVLTSINNA +RR LGFLSCG GNPID
Sbjct: 4   NPIFVLLIIFATLLLQMSSISSSPVADPETIVEEVLTSINNA-SRRKLGFLSCGTGNPID 63

Query: 63  DCWRCDPNWEKNRKRLADCAIGFGKRAIGGKNGKFYIVTDPSDKDVVNPKPGTLRHAVIQ 122
           DCWRCDPNWEKNRKRLADC IGFGKRAIGGK+GKFY+VTDPSDKDVVNPKPGTLRHAVIQ
Sbjct: 64  DCWRCDPNWEKNRKRLADCGIGFGKRAIGGKDGKFYVVTDPSDKDVVNPKPGTLRHAVIQ 123

Query: 123 KEPLWIIFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTNIIIHGINIHD 182
           KEPLWI FKRDMVIKLKAELLMNSFKTIDGRG SVHIAGGPCITIQFVTNIIIHGINIHD
Sbjct: 124 KEPLWITFKRDMVIKLKAELLMNSFKTIDGRGASVHIAGGPCITIQFVTNIIIHGINIHD 183

Query: 183 CKQGGNTYVRDSPEHYGWRTLSDGDGVSIFGGSHIWVDHCSLSNCRDGLIDAIHGSTAIT 242
           CKQGGNTYVRDSP+HYGWRTLSDGDGVSIFGG+HIW+DHCSLSNCRDGLIDAIHGSTAIT
Sbjct: 184 CKQGGNTYVRDSPDHYGWRTLSDGDGVSIFGGTHIWIDHCSLSNCRDGLIDAIHGSTAIT 243

Query: 243 LSNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYT 302
           +SNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYT
Sbjct: 244 ISNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYT 303

Query: 303 HWIMYAIGGSASPTINSQGNRYLAPNNQDRKEVTKREDAPQSKWKNWNWRSDGDLMLNGA 362
           HWIMYAIGGSASPTINSQGNRYLAPN+Q+ KEVTK EDAPQS+WKNWNWRS GDLMLNGA
Sbjct: 304 HWIMYAIGGSASPTINSQGNRYLAPNDQNNKEVTKHEDAPQSQWKNWNWRSSGDLMLNGA 363

Query: 363 FFTRSGAGASSSYARASSLSAKSSSLVSSITAGAGVLRCRKGSKC 408
           FFT SGAGASSSYARASSLSAKSSSLV S+TA AGVLRCRKGS+C
Sbjct: 364 FFTMSGAGASSSYARASSLSAKSSSLVGSMTATAGVLRCRKGSRC 407

[Close]

BLAST of Lsi08G016280 vs. ExPASy TrEMBL
Match: A0A5A7V5E9 (Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G001810 PE=3 SV=1)

HSP 1 Score: 770.8 bits (1989), Expect = 2.9e-219
Identity = 365/385 (94.81%), Postives = 377/385 (97.92%), Query Frame = 0


Query: 23  SSSPVADPEVVVQEVLTSINNATTRRNLGFLSCGIGNPIDDCWRCDPNWEKNRKRLADCA 82
           SSSPV DP++V QEVLTSINNATTRRNLGFLSC  GNPIDDCWRCD NWEKNRKRLADCA
Sbjct: 6   SSSPVPDPDLVAQEVLTSINNATTRRNLGFLSCRTGNPIDDCWRCDANWEKNRKRLADCA 65

Query: 83  IGFGKRAIGGKNGKFYIVTDPSDKDVVNPKPGTLRHAVIQKEPLWIIFKRDMVIKLKAEL 142
           IGFGKRAIGGKNG++YIVTDPSD DVVNPKPGTLRHAVIQKEPLWIIFKRDMVIKLKAEL
Sbjct: 66  IGFGKRAIGGKNGRYYIVTDPSDNDVVNPKPGTLRHAVIQKEPLWIIFKRDMVIKLKAEL 125

Query: 143 LMNSFKTIDGRGVSVHIAGGPCITIQFVTNIIIHGINIHDCKQGGNTYVRDSPEHYGWRT 202
           LMNSFKTIDGRGVSVHIAGGPCITIQFVTNIIIHGINIHDCKQGGNTYVRDSPEHYGWRT
Sbjct: 126 LMNSFKTIDGRGVSVHIAGGPCITIQFVTNIIIHGINIHDCKQGGNTYVRDSPEHYGWRT 185

Query: 203 LSDGDGVSIFGGSHIWVDHCSLSNCRDGLIDAIHGSTAITLSNNYMTHHNKVMLLGHSDS 262
           +SDGDG+SIFGGSH+W+DHCSLSNCRDGLIDAIHGSTAIT+SNNYMTHHNKVMLLGHSDS
Sbjct: 186 VSDGDGISIFGGSHVWIDHCSLSNCRDGLIDAIHGSTAITVSNNYMTHHNKVMLLGHSDS 245

Query: 263 YTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWIMYAIGGSASPTINSQGN 322
           YTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWIMYAIGGSASPTINSQGN
Sbjct: 246 YTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWIMYAIGGSASPTINSQGN 305

Query: 323 RYLAPNNQDRKEVTKREDAPQSKWKNWNWRSDGDLMLNGAFFTRSGAGASSSYARASSLS 382
           RYLAPNNQDRKEVTKREDAPQSKWKNWNWRSDGDLMLNGAFF RSGAGASS YARASSLS
Sbjct: 306 RYLAPNNQDRKEVTKREDAPQSKWKNWNWRSDGDLMLNGAFFVRSGAGASSRYARASSLS 365

Query: 383 AKSSSLVSSITAGAGVLRCRKGSKC 408
           AKSSSLVSSIT+GAGVL+CRKGS+C
Sbjct: 366 AKSSSLVSSITSGAGVLKCRKGSRC 390

[Close]

BLAST of Lsi08G016280 vs. NCBI nr
Match: XP_038885657.1 (probable pectate lyase 5 [Benincasa hispida])

HSP 1 Score: 819.3 bits (2115), Expect = 1.5e-233
Identity = 395/408 (96.81%), Postives = 403/408 (98.77%), Query Frame = 0


Query: 1   MENP-IFLLIITFFSVLLQMWSVSSSPVADPEVVVQEVLTSINNATTRRNLGFLSCGIGN 60
           MENP IFLLIITFFSVLLQ+WSVSSSP+ADPEVVVQEVLTSINNAT RRNLGFLSCG GN
Sbjct: 1   MENPIIFLLIITFFSVLLQIWSVSSSPIADPEVVVQEVLTSINNATARRNLGFLSCGTGN 60

Query: 61  PIDDCWRCDPNWEKNRKRLADCAIGFGKRAIGGKNGKFYIVTDPSDKDVVNPKPGTLRHA 120
           PIDDCWRCDPNWE NRKRLADCAIGFGKRAIGGKNGK+Y+VTDPSDKDVVNPKPGTLRHA
Sbjct: 61  PIDDCWRCDPNWENNRKRLADCAIGFGKRAIGGKNGKYYVVTDPSDKDVVNPKPGTLRHA 120

Query: 121 VIQKEPLWIIFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTNIIIHGIN 180
           VIQKEPLWIIFKRDMVIKLKAELLMNSFKTIDGRG SVHIAGGPCITIQFVTNIIIHGIN
Sbjct: 121 VIQKEPLWIIFKRDMVIKLKAELLMNSFKTIDGRGASVHIAGGPCITIQFVTNIIIHGIN 180

Query: 181 IHDCKQGGNTYVRDSPEHYGWRTLSDGDGVSIFGGSHIWVDHCSLSNCRDGLIDAIHGST 240
           IHDCKQGGNTYVRDSPEHYGWRT+SDGDGVSIFGGSHIWVDHCSLSNCRDGLIDAIHGST
Sbjct: 181 IHDCKQGGNTYVRDSPEHYGWRTVSDGDGVSIFGGSHIWVDHCSLSNCRDGLIDAIHGST 240

Query: 241 AITLSNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNN 300
           AITLSNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNN
Sbjct: 241 AITLSNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNN 300

Query: 301 DYTHWIMYAIGGSASPTINSQGNRYLAPNNQDRKEVTKREDAPQSKWKNWNWRSDGDLML 360
           DYTHWIMYAIGGSASPTINSQGNRYLAPNN+DRKEVTKREDAPQSKWKNWNWRSDGDLML
Sbjct: 301 DYTHWIMYAIGGSASPTINSQGNRYLAPNNKDRKEVTKREDAPQSKWKNWNWRSDGDLML 360

Query: 361 NGAFFTRSGAGASSSYARASSLSAKSSSLVSSITAGAGVLRCRKGSKC 408
           NGAFFTRSGAGASSSYARASSLSAKSSSLVSS+TAGAGVLRCRKGS+C
Sbjct: 361 NGAFFTRSGAGASSSYARASSLSAKSSSLVSSMTAGAGVLRCRKGSRC 408

[Close]

BLAST of Lsi08G016280 vs. NCBI nr
Match: XP_022996659.1 (probable pectate lyase 5 [Cucurbita maxima])

HSP 1 Score: 797.0 bits (2057), Expect = 7.8e-227
Identity = 383/407 (94.10%), Postives = 398/407 (97.79%), Query Frame = 0


Query: 1   MENPIFLLIITFFSVLLQMWSVSSSPVADPEVVVQEVLTSINNATTRRNLGFLSCGIGNP 60
           ME P FL IIT F++LLQM S+SSSPVADPE+VVQEVL+SINNATTRRNLGFLSCG GNP
Sbjct: 1   MEIPSFLPIIT-FTLLLQMCSISSSPVADPELVVQEVLSSINNATTRRNLGFLSCGTGNP 60

Query: 61  IDDCWRCDPNWEKNRKRLADCAIGFGKRAIGGKNGKFYIVTDPSDKDVVNPKPGTLRHAV 120
           IDDCWRCDPNWEKNRKRLADC IGFGKRAIGGKNGKFY+VTDPSDKDVVNPKPGTLRHAV
Sbjct: 61  IDDCWRCDPNWEKNRKRLADCGIGFGKRAIGGKNGKFYVVTDPSDKDVVNPKPGTLRHAV 120

Query: 121 IQKEPLWIIFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTNIIIHGINI 180
           IQKEPLWI FKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQ+VTNIIIHGINI
Sbjct: 121 IQKEPLWITFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQYVTNIIIHGINI 180

Query: 181 HDCKQGGNTYVRDSPEHYGWRTLSDGDGVSIFGGSHIWVDHCSLSNCRDGLIDAIHGSTA 240
           HDCKQGGNTYVRDSPEHYGWRTLSDGDGVSIFGGSH+W+DHCSLSNCRDGLIDAIHGSTA
Sbjct: 181 HDCKQGGNTYVRDSPEHYGWRTLSDGDGVSIFGGSHVWIDHCSLSNCRDGLIDAIHGSTA 240

Query: 241 ITLSNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNND 300
           IT+SNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNND
Sbjct: 241 ITVSNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNND 300

Query: 301 YTHWIMYAIGGSASPTINSQGNRYLAPNNQDRKEVTKREDAPQSKWKNWNWRSDGDLMLN 360
           YTHWIMYAIGGSASPTINSQGNRYLAPNN+DRKEVTKREDAPQSKWKNWNWRS GDLMLN
Sbjct: 301 YTHWIMYAIGGSASPTINSQGNRYLAPNNRDRKEVTKREDAPQSKWKNWNWRSSGDLMLN 360

Query: 361 GAFFTRSGAGASSSYARASSLSAKSSSLVSSITAGAGVLRCRKGSKC 408
           GAFFTRSGAG+SSSYARASSLSAKSSSLVS++TAGAGVLRCRKGS+C
Sbjct: 361 GAFFTRSGAGSSSSYARASSLSAKSSSLVSTMTAGAGVLRCRKGSRC 406

[Close]

BLAST of Lsi08G016280 vs. NCBI nr
Match: KAG7029228.1 (putative pectate lyase 5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 795.8 bits (2054), Expect = 1.7e-226
Identity = 382/407 (93.86%), Postives = 398/407 (97.79%), Query Frame = 0


Query: 1   MENPIFLLIITFFSVLLQMWSVSSSPVADPEVVVQEVLTSINNATTRRNLGFLSCGIGNP 60
           ME P FL IIT F++LLQM ++SSSPVADPE+VVQEVL+SINNATTRRNLGFLSCG GNP
Sbjct: 1   MEIPSFLPIIT-FTLLLQMCAISSSPVADPELVVQEVLSSINNATTRRNLGFLSCGTGNP 60

Query: 61  IDDCWRCDPNWEKNRKRLADCAIGFGKRAIGGKNGKFYIVTDPSDKDVVNPKPGTLRHAV 120
           IDDCWRCDPNWEKNRKRLADC IGFGKRAIGGKNGKFY+VTDPSDKDVVNPKPGTLRHAV
Sbjct: 61  IDDCWRCDPNWEKNRKRLADCGIGFGKRAIGGKNGKFYVVTDPSDKDVVNPKPGTLRHAV 120

Query: 121 IQKEPLWIIFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTNIIIHGINI 180
           IQKEPLWI FKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQ+VTNIIIHGINI
Sbjct: 121 IQKEPLWITFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQYVTNIIIHGINI 180

Query: 181 HDCKQGGNTYVRDSPEHYGWRTLSDGDGVSIFGGSHIWVDHCSLSNCRDGLIDAIHGSTA 240
           HDCKQGGNTYVRDSPEHYGWRTLSDGDGVSIFGGSH+W+DHCSLSNCRDGLIDAIHGSTA
Sbjct: 181 HDCKQGGNTYVRDSPEHYGWRTLSDGDGVSIFGGSHVWIDHCSLSNCRDGLIDAIHGSTA 240

Query: 241 ITLSNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNND 300
           IT+SNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNND
Sbjct: 241 ITVSNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNND 300

Query: 301 YTHWIMYAIGGSASPTINSQGNRYLAPNNQDRKEVTKREDAPQSKWKNWNWRSDGDLMLN 360
           YTHWIMYAIGGSASPTINSQGNRYLAPNN+DRKEVTKREDAPQSKWKNWNWRS GDLMLN
Sbjct: 301 YTHWIMYAIGGSASPTINSQGNRYLAPNNRDRKEVTKREDAPQSKWKNWNWRSSGDLMLN 360

Query: 361 GAFFTRSGAGASSSYARASSLSAKSSSLVSSITAGAGVLRCRKGSKC 408
           GAFFTRSGAG+SSSYARASSLSAKSSSLVS++TAGAGVLRCRKGS+C
Sbjct: 361 GAFFTRSGAGSSSSYARASSLSAKSSSLVSTMTAGAGVLRCRKGSRC 406

[Close]

BLAST of Lsi08G016280 vs. NCBI nr
Match: XP_023545498.1 (probable pectate lyase 5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 794.7 bits (2051), Expect = 3.9e-226
Identity = 381/407 (93.61%), Postives = 398/407 (97.79%), Query Frame = 0


Query: 1   MENPIFLLIITFFSVLLQMWSVSSSPVADPEVVVQEVLTSINNATTRRNLGFLSCGIGNP 60
           ME P FL IIT F++LLQM ++SSSPV+DPE+VVQEVL+SINNATTRRNLGFLSCG GNP
Sbjct: 1   MEIPSFLPIIT-FTLLLQMCAISSSPVSDPELVVQEVLSSINNATTRRNLGFLSCGTGNP 60

Query: 61  IDDCWRCDPNWEKNRKRLADCAIGFGKRAIGGKNGKFYIVTDPSDKDVVNPKPGTLRHAV 120
           IDDCWRCDPNWEKNRKRLADC IGFGKRAIGGKNGKFY+VTDPSDKDVVNPKPGTLRHAV
Sbjct: 61  IDDCWRCDPNWEKNRKRLADCGIGFGKRAIGGKNGKFYVVTDPSDKDVVNPKPGTLRHAV 120

Query: 121 IQKEPLWIIFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTNIIIHGINI 180
           IQKEPLWI FKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQ+VTNIIIHGINI
Sbjct: 121 IQKEPLWITFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQYVTNIIIHGINI 180

Query: 181 HDCKQGGNTYVRDSPEHYGWRTLSDGDGVSIFGGSHIWVDHCSLSNCRDGLIDAIHGSTA 240
           HDCKQGGNTYVRDSPEHYGWRTLSDGDGVSIFGGSH+W+DHCSLSNCRDGLIDAIHGSTA
Sbjct: 181 HDCKQGGNTYVRDSPEHYGWRTLSDGDGVSIFGGSHVWIDHCSLSNCRDGLIDAIHGSTA 240

Query: 241 ITLSNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNND 300
           IT+SNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNND
Sbjct: 241 ITVSNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNND 300

Query: 301 YTHWIMYAIGGSASPTINSQGNRYLAPNNQDRKEVTKREDAPQSKWKNWNWRSDGDLMLN 360
           YTHWIMYAIGGSASPTINSQGNRYLAPNN+DRKEVTKREDAPQSKWKNWNWRS GDLMLN
Sbjct: 301 YTHWIMYAIGGSASPTINSQGNRYLAPNNRDRKEVTKREDAPQSKWKNWNWRSSGDLMLN 360

Query: 361 GAFFTRSGAGASSSYARASSLSAKSSSLVSSITAGAGVLRCRKGSKC 408
           GAFFTRSGAG+SSSYARASSLSAKSSSLVS++TAGAGVLRCRKGS+C
Sbjct: 361 GAFFTRSGAGSSSSYARASSLSAKSSSLVSTMTAGAGVLRCRKGSRC 406

[Close]

BLAST of Lsi08G016280 vs. NCBI nr
Match: XP_022962436.1 (probable pectate lyase 5 [Cucurbita moschata])

HSP 1 Score: 794.7 bits (2051), Expect = 3.9e-226
Identity = 381/407 (93.61%), Postives = 398/407 (97.79%), Query Frame = 0


Query: 1   MENPIFLLIITFFSVLLQMWSVSSSPVADPEVVVQEVLTSINNATTRRNLGFLSCGIGNP 60
           ME P FL IIT F++LLQM ++SSSPVADP++VVQEVL+SINNATTRRNLGFLSCG GNP
Sbjct: 1   MEIPSFLPIIT-FTLLLQMCAISSSPVADPQLVVQEVLSSINNATTRRNLGFLSCGTGNP 60

Query: 61  IDDCWRCDPNWEKNRKRLADCAIGFGKRAIGGKNGKFYIVTDPSDKDVVNPKPGTLRHAV 120
           IDDCWRCDPNWEKNRKRLADC IGFGKRAIGGKNGKFY+VTDPSDKDVVNPKPGTLRHAV
Sbjct: 61  IDDCWRCDPNWEKNRKRLADCGIGFGKRAIGGKNGKFYVVTDPSDKDVVNPKPGTLRHAV 120

Query: 121 IQKEPLWIIFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTNIIIHGINI 180
           IQKEPLWI FKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQ+VTNIIIHGINI
Sbjct: 121 IQKEPLWITFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQYVTNIIIHGINI 180

Query: 181 HDCKQGGNTYVRDSPEHYGWRTLSDGDGVSIFGGSHIWVDHCSLSNCRDGLIDAIHGSTA 240
           HDCKQGGNTYVRDSPEHYGWRTLSDGDGVSIFGGSH+W+DHCSLSNCRDGLIDAIHGSTA
Sbjct: 181 HDCKQGGNTYVRDSPEHYGWRTLSDGDGVSIFGGSHVWIDHCSLSNCRDGLIDAIHGSTA 240

Query: 241 ITLSNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNND 300
           IT+SNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNND
Sbjct: 241 ITVSNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNND 300

Query: 301 YTHWIMYAIGGSASPTINSQGNRYLAPNNQDRKEVTKREDAPQSKWKNWNWRSDGDLMLN 360
           YTHWIMYAIGGSASPTINSQGNRYLAPNN+DRKEVTKREDAPQSKWKNWNWRS GDLMLN
Sbjct: 301 YTHWIMYAIGGSASPTINSQGNRYLAPNNRDRKEVTKREDAPQSKWKNWNWRSSGDLMLN 360

Query: 361 GAFFTRSGAGASSSYARASSLSAKSSSLVSSITAGAGVLRCRKGSKC 408
           GAFFTRSGAG+SSSYARASSLSAKSSSLVS++TAGAGVLRCRKGS+C
Sbjct: 361 GAFFTRSGAGSSSSYARASSLSAKSSSLVSTMTAGAGVLRCRKGSRC 406

[Close]

BLAST of Lsi08G016280 vs. TAIR 10
Match: AT1G67750.1 (Pectate lyase family protein )

HSP 1 Score: 678.3 bits (1749), Expect = 3.8e-195
Identity = 324/403 (80.40%), Postives = 359/403 (89.08%), Query Frame = 0


Query: 8   LIITFFSVLLQMWS---VSSSPVADPEVVVQEVLTSINNATTRRNLGFLSCGIGNPIDDC 67
           L ++ FS LL + S   ++S+PV++PE+VVQEV   IN   +RRNLG LSCG GNPIDDC
Sbjct: 8   LSLSLFSCLLLVLSPTFIASTPVSEPELVVQEVNEKIN--ASRRNLGVLSCGTGNPIDDC 67

Query: 68  WRCDPNWEKNRKRLADCAIGFGKRAIGGKNGKFYIVTDPSDKDVVNPKPGTLRHAVIQKE 127
           WRCDP WEKNR+RLADCAIGFGK AIGG++GK Y+VTD SDKDVVNPKPGTLRHAVIQ E
Sbjct: 68  WRCDPKWEKNRQRLADCAIGFGKHAIGGRDGKIYVVTDSSDKDVVNPKPGTLRHAVIQDE 127

Query: 128 PLWIIFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTNIIIHGINIHDCK 187
           PLWIIF RDMVIKLK EL+MNSFKTIDGRG SVHIAGG CIT+Q+VTNIIIHG+NIHDCK
Sbjct: 128 PLWIIFARDMVIKLKEELIMNSFKTIDGRGASVHIAGGACITVQYVTNIIIHGVNIHDCK 187

Query: 188 QGGNTYVRDSPEHYGWRTLSDGDGVSIFGGSHIWVDHCSLSNCRDGLIDAIHGSTAITLS 247
           + GN YVRDSP HYGWRT SDGD VSIFGGSH+WVDHCSLSNC DGLIDAIHGSTAIT+S
Sbjct: 188 RKGNAYVRDSPSHYGWRTASDGDAVSIFGGSHVWVDHCSLSNCADGLIDAIHGSTAITIS 247

Query: 248 NNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW 307
           NNY++HHNKVMLLGHSDSYT+DKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW
Sbjct: 248 NNYLSHHNKVMLLGHSDSYTRDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW 307

Query: 308 IMYAIGGSASPTINSQGNRYLAPNNQDRKEVTKREDAPQSKWKNWNWRSDGDLMLNGAFF 367
            MYAIGGSA+PTINSQGNR+LAPN+   KEVTK EDAP+SKWK WNWRS+GDL LNGAFF
Sbjct: 308 QMYAIGGSAAPTINSQGNRFLAPNDHVFKEVTKYEDAPRSKWKKWNWRSEGDLFLNGAFF 367

Query: 368 TRSGAGASSSYARASSLSAKSSSLVSSITAGAGVLRCRKGSKC 408
           T SG GASSSYA+ASSLSA+ SSLV+S+T+ AG L CRKGS+C
Sbjct: 368 TPSGGGASSSYAKASSLSARPSSLVASVTSNAGALFCRKGSRC 408

[Close]

BLAST of Lsi08G016280 vs. TAIR 10
Match: AT5G63180.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 655.6 bits (1690), Expect = 2.6e-188
Identity = 306/400 (76.50%), Postives = 350/400 (87.50%), Query Frame = 0


Query: 10  ITFFSVLLQMWSVSSSPVADPEVVVQEVLTSINNATTRRNLGFLSCGIGNPIDDCWRCDP 69
           + FF  +L     SS PV+DPE+VV+EV   IN + +RR LGF SCG GNPIDDCWRCD 
Sbjct: 33  LIFFCCILFSALASSLPVSDPELVVEEVHRKINESISRRKLGFFSCGSGNPIDDCWRCDK 92

Query: 70  NWEKNRKRLADCAIGFGKRAIGGKNGKFYIVTDPSDKDVVNPKPGTLRHAVIQKEPLWII 129
           +WEKNRKRLADC IGFGK AIGG++G+ Y+VTDP + D VNP+PGTLR+AVIQ EPLWII
Sbjct: 93  DWEKNRKRLADCGIGFGKNAIGGRDGEIYVVTDPGNDDPVNPRPGTLRYAVIQDEPLWII 152

Query: 130 FKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTNIIIHGINIHDCKQGGNT 189
           FKRDM I+LK EL+MNSFKT+DGRG SVHI+GGPCITIQ+VTNIIIHG++IHDCKQGGNT
Sbjct: 153 FKRDMTIQLKEELIMNSFKTLDGRGASVHISGGPCITIQYVTNIIIHGLHIHDCKQGGNT 212

Query: 190 YVRDSPEHYGWRTLSDGDGVSIFGGSHIWVDHCSLSNCRDGLIDAIHGSTAITLSNNYMT 249
           YVRDSPEHYG+RT+SDGDGVSIFGGSH+WVDHCSLSNC DGLIDAI GSTAIT+SNNY+T
Sbjct: 213 YVRDSPEHYGYRTVSDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIRGSTAITISNNYLT 272

Query: 250 HHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWIMYAI 309
           HHNKVMLLGHSD+Y QDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW MYAI
Sbjct: 273 HHNKVMLLGHSDTYEQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAI 332

Query: 310 GGSASPTINSQGNRYLAPNNQDRKEVTKREDAPQSKWKNWNWRSDGDLMLNGAFFTRSGA 369
           GGSA+PTINSQGNR+LAP++   KEVTK EDAP+ +W+NWNWRS+GDL+LNGAFFT SGA
Sbjct: 333 GGSANPTINSQGNRFLAPDDSSSKEVTKHEDAPEDEWRNWNWRSEGDLLLNGAFFTYSGA 392

Query: 370 G--ASSSYARASSLSAKSSSLVSSITAGAGVLRCRKGSKC 408
           G   SSSY++ASSL+A+ SS V  IT  +G L C++GS C
Sbjct: 393 GPAKSSSYSKASSLAARPSSHVGEITIASGALSCKRGSHC 432

[Close]

BLAST of Lsi08G016280 vs. TAIR 10
Match: AT4G24780.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 642.9 bits (1657), Expect = 1.8e-184
Identity = 306/401 (76.31%), Postives = 346/401 (86.28%), Query Frame = 0


Query: 8   LIITFFSVLLQMWSVSSSPVADPEVVVQEVLTSIN-NATTRRNLGFLSCGIGNPIDDCWR 67
           L IT  S LL      SSPV DPE VV+EV  SIN +   RR LG+LSC  GNPIDDCWR
Sbjct: 8   LFITIVSFLLYAPLFLSSPVPDPESVVEEVHKSINASVAGRRKLGYLSCTTGNPIDDCWR 67

Query: 68  CDPNWEKNRKRLADCAIGFGKRAIGGKNGKFYIVTDPSDKDVVNPKPGTLRHAVIQKEPL 127
           CDP+WE++R+RLADCAIGFGK AIGG++G+ Y+VTD  + + V+PKPGTLRHAV+Q EPL
Sbjct: 68  CDPHWEQHRQRLADCAIGFGKNAIGGRDGRIYVVTDSGNDNPVSPKPGTLRHAVVQDEPL 127

Query: 128 WIIFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTNIIIHGINIHDCKQG 187
           WIIF+RDM I+LK EL+MNSFKTIDGRG SVHI+GGPCITIQ+VTNIIIHGI+IHDCKQG
Sbjct: 128 WIIFQRDMTIQLKEELIMNSFKTIDGRGASVHISGGPCITIQYVTNIIIHGIHIHDCKQG 187

Query: 188 GNTYVRDSPEHYGWRTLSDGDGVSIFGGSHIWVDHCSLSNCRDGLIDAIHGSTAITLSNN 247
           GN  VR SP H+GWRT+SDGDGVSIFGGSH+WVDHCS SNC DGLIDAI GSTAITLSNN
Sbjct: 188 GNAMVRSSPRHFGWRTISDGDGVSIFGGSHVWVDHCSFSNCEDGLIDAIMGSTAITLSNN 247

Query: 248 YMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWIM 307
           +MTHH+KVMLLGHSD+Y++DKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW M
Sbjct: 248 HMTHHDKVMLLGHSDTYSRDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEM 307

Query: 308 YAIGGSASPTINSQGNRYLAPNNQDRKEVTKREDAPQSKWKNWNWRSDGDLMLNGAFFTR 367
           YAIGGSA+PTINSQGNR+LAPN +  KEVTK EDAP+S+WK WNWRS GDL+LNGAFFT 
Sbjct: 308 YAIGGSANPTINSQGNRFLAPNIRFSKEVTKHEDAPESEWKRWNWRSSGDLLLNGAFFTP 367

Query: 368 SGAGASSSYARASSLSAKSSSLVSSITAGAGVLRCRKGSKC 408
           SG  ASSSYA+ASSL AK SSLV  +T+ +G L CRKGS+C
Sbjct: 368 SGGAASSSYAKASSLGAKPSSLVGPLTSTSGALNCRKGSRC 408

[Close]

BLAST of Lsi08G016280 vs. TAIR 10
Match: AT4G24780.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 642.9 bits (1657), Expect = 1.8e-184
Identity = 306/401 (76.31%), Postives = 346/401 (86.28%), Query Frame = 0


Query: 8   LIITFFSVLLQMWSVSSSPVADPEVVVQEVLTSIN-NATTRRNLGFLSCGIGNPIDDCWR 67
           L IT  S LL      SSPV DPE VV+EV  SIN +   RR LG+LSC  GNPIDDCWR
Sbjct: 8   LFITIVSFLLYAPLFLSSPVPDPESVVEEVHKSINASVAGRRKLGYLSCTTGNPIDDCWR 67

Query: 68  CDPNWEKNRKRLADCAIGFGKRAIGGKNGKFYIVTDPSDKDVVNPKPGTLRHAVIQKEPL 127
           CDP+WE++R+RLADCAIGFGK AIGG++G+ Y+VTD  + + V+PKPGTLRHAV+Q EPL
Sbjct: 68  CDPHWEQHRQRLADCAIGFGKNAIGGRDGRIYVVTDSGNDNPVSPKPGTLRHAVVQDEPL 127

Query: 128 WIIFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTNIIIHGINIHDCKQG 187
           WIIF+RDM I+LK EL+MNSFKTIDGRG SVHI+GGPCITIQ+VTNIIIHGI+IHDCKQG
Sbjct: 128 WIIFQRDMTIQLKEELIMNSFKTIDGRGASVHISGGPCITIQYVTNIIIHGIHIHDCKQG 187

Query: 188 GNTYVRDSPEHYGWRTLSDGDGVSIFGGSHIWVDHCSLSNCRDGLIDAIHGSTAITLSNN 247
           GN  VR SP H+GWRT+SDGDGVSIFGGSH+WVDHCS SNC DGLIDAI GSTAITLSNN
Sbjct: 188 GNAMVRSSPRHFGWRTISDGDGVSIFGGSHVWVDHCSFSNCEDGLIDAIMGSTAITLSNN 247

Query: 248 YMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWIM 307
           +MTHH+KVMLLGHSD+Y++DKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW M
Sbjct: 248 HMTHHDKVMLLGHSDTYSRDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEM 307

Query: 308 YAIGGSASPTINSQGNRYLAPNNQDRKEVTKREDAPQSKWKNWNWRSDGDLMLNGAFFTR 367
           YAIGGSA+PTINSQGNR+LAPN +  KEVTK EDAP+S+WK WNWRS GDL+LNGAFFT 
Sbjct: 308 YAIGGSANPTINSQGNRFLAPNIRFSKEVTKHEDAPESEWKRWNWRSSGDLLLNGAFFTP 367

Query: 368 SGAGASSSYARASSLSAKSSSLVSSITAGAGVLRCRKGSKC 408
           SG  ASSSYA+ASSL AK SSLV  +T+ +G L CRKGS+C
Sbjct: 368 SGGAASSSYAKASSLGAKPSSLVGPLTSTSGALNCRKGSRC 408

[Close]

BLAST of Lsi08G016280 vs. TAIR 10
Match: AT3G27400.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 610.1 bits (1572), Expect = 1.3e-174
Identity = 290/402 (72.14%), Postives = 335/402 (83.33%), Query Frame = 0


Query: 6   FLLIITFFSVLLQMWSVSSSPVADPEVVVQEVLTSINNATTRRNLGFLSCGIGNPIDDCW 65
           FLL++T  + L    S+    V DP +VV +V  S+ NA +RR+L +LSC  GNPIDDCW
Sbjct: 13  FLLLLTIGNTLAFSSSLPDH-VQDPNLVVDDVNRSVFNA-SRRSLAYLSCRTGNPIDDCW 72

Query: 66  RCDPNWEKNRKRLADCAIGFGKRAIGGKNGKFYIVTDPSDKDVVNPKPGTLRHAVIQKEP 125
           RCDPNWE NR+RLADCAIGFGK AIGG+ G+ Y+VTDP++ D VNP+PGTLR+AV Q+EP
Sbjct: 73  RCDPNWETNRQRLADCAIGFGKNAIGGRKGRIYVVTDPANDDPVNPRPGTLRYAVTQEEP 132

Query: 126 LWIIFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTNIIIHGINIHDCKQ 185
           LWIIFKRDMVI+LK EL++ SFKTIDGRG SVHI  GPC+ I + TNIIIHGINIHDCK 
Sbjct: 133 LWIIFKRDMVIRLKKELIITSFKTIDGRGSSVHITDGPCLKIHYATNIIIHGINIHDCKP 192

Query: 186 GGNTYVRDSPEHYGWRTLSDGDGVSIFGGSHIWVDHCSLSNCRDGLIDAIHGSTAITLSN 245
           G    ++D P H GW   SDGD V+IFGG H+W+DHCSLSNC DGLIDAIHGSTAIT+SN
Sbjct: 193 GSGGMIKDGPHHTGWWMQSDGDAVAIFGGKHVWIDHCSLSNCDDGLIDAIHGSTAITISN 252

Query: 246 NYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWI 305
           N+MTHH+KVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW 
Sbjct: 253 NHMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWE 312

Query: 306 MYAIGGSASPTINSQGNRYLAPNNQDRKEVTKREDAPQSKWKNWNWRSDGDLMLNGAFFT 365
           MYAIGGSASPTI SQGNR+LAPN +  KEVTK EDAP+SKW++WNWRS+GD++LNGA+F 
Sbjct: 313 MYAIGGSASPTIYSQGNRFLAPNTRFNKEVTKHEDAPESKWRDWNWRSEGDMLLNGAYFR 372

Query: 366 RSGAGASSSYARASSLSAKSSSLVSSITAGAGVLRCRKGSKC 408
            SGA A S+YARASSLSA+ SSLV SIT  AG L CR+G +C
Sbjct: 373 ESGAEAPSTYARASSLSARPSSLVGSITTTAGTLSCRRGRRC 412

The following BLAST results are available for this feature:

BLAST of Lsi08G016280 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FXD8	5.3e-194	80.40	Probable pectate lyase 5 OS=Arabidopsis thaliana OX=3702 GN=At1g67750 PE=2 SV=2	[more]
Q93Z25	3.7e-187	76.50	Probable pectate lyase 22 OS=Arabidopsis thaliana OX=3702 GN=At5g63180 PE=2 SV=1	[more]
Q9C5M8	2.5e-183	76.31	Probable pectate lyase 18 OS=Arabidopsis thaliana OX=3702 GN=At4g24780 PE=2 SV=2	[more]
O24554	3.2e-175	74.06	Pectate lyase OS=Zinnia violacea OX=34245 PE=1 SV=1	[more]
Q9LTZ0	1.8e-173	72.14	Putative pectate lyase 11 OS=Arabidopsis thaliana OX=3702 GN=At3g27400 PE=3 SV=2	[more]

BLAST of Lsi08G016280 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1K9C7	3.8e-227	94.10	Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111491836 PE=3 SV=1	[more]
A0A6J1HD44	1.9e-226	93.61	Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111462874 PE=3 SV=1	[more]
A0A1S3BB18	1.4e-221	90.60	Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103487738 PE=3 SV=1	[more]
A0A6J1BR07	4.5e-220	91.36	Pectate lyase OS=Momordica charantia OX=3673 GN=LOC111004807 PE=3 SV=1	[more]
A0A5A7V5E9	2.9e-219	94.81	Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G001810...	[more]

BLAST of Lsi08G016280 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038885657.1	1.5e-233	96.81	probable pectate lyase 5 [Benincasa hispida]	[more]
XP_022996659.1	7.8e-227	94.10	probable pectate lyase 5 [Cucurbita maxima]	[more]
KAG7029228.1	1.7e-226	93.86	putative pectate lyase 5 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023545498.1	3.9e-226	93.61	probable pectate lyase 5 [Cucurbita pepo subsp. pepo]	[more]
XP_022962436.1	3.9e-226	93.61	probable pectate lyase 5 [Cucurbita moschata]	[more]

BLAST of Lsi08G016280 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G67750.1	3.8e-195	80.40	Pectate lyase family protein	[more]
AT5G63180.1	2.6e-188	76.50	Pectin lyase-like superfamily protein	[more]
AT4G24780.1	1.8e-184	76.31	Pectin lyase-like superfamily protein	[more]
AT4G24780.2	1.8e-184	76.31	Pectin lyase-like superfamily protein	[more]
AT3G27400.1	1.3e-174	72.14	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR018082	AmbAllergen	PRINTS	PR00807	AMBALLERGEN	coord: 105..130 score: 68.32 coord: 141..157 score: 66.67 coord: 349..373 score: 52.38 coord: 285..304 score: 78.1 coord: 205..226 score: 59.09 coord: 378..401 score: 37.7 coord: 81..98 score: 61.11 coord: 307..326 score: 74.52
IPR002022	Pectate lyase	SMART	SM00656	amb_all	coord: 133..330 e-value: 5.6E-102 score: 354.8
IPR002022	Pectate lyase	PFAM	PF00544	Pectate_lyase_4	coord: 167..322 e-value: 3.9E-18 score: 65.8
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 58..400 e-value: 2.1E-117 score: 394.0
IPR045032	Pectin lyase family	PANTHER	PTHR31683	PECTATE LYASE 18-RELATED	coord: 14..404
None	No IPR available	PANTHER	PTHR31683:SF151	PECTATE LYASE	coord: 14..404
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 40..402

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi08G016280.1	Lsi08G016280.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0030570	pectate lyase activity