Lsi08G014990 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi08G014990
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Domain of unknown function (DUF303)
Location	chr08: 23136585 .. 23137415 (+)
RNA-Seq Expression	Lsi08G014990
Synteny	Lsi08G014990

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATATTTGGCACTTCCCTCTCAGGGGCTACTTCTCCTAAGAACATATTCATCCTCGCTGGTCAGAGTAATATGGCTGGTCGAGGTGGGGTTGAGAACAATCTATTGGGAAAGCTCGAGTGGGATGGATTGATCCCACCAGAATGTCAATCTGACCCATCCATCCTACGATTGAACCCTGCTCGCCAATGGGAGATAGCACGAGAGCCTCTCCATGAGGGGATCGACATCAACAAGACAGTTGGGATTGGTCCGGGAATGTCATTTGCTCACCAACTGCTAGCTAAAGCAGGCCCAAATGCAGGTGTTGTGGGCTTAGTTCCTTGTGCTAGAGGTGGCACTTTAATTGAACAATGGATTAAAAATCCTAGCAATCCTAATGCAACTTTTTACCAAAATTTCATTGAACGAATCAAAGCATCAGATAAAGATGGTGGAGTTGTGCGTGCTCTTTTCTGGTTTCAAGGTGAGAGTGATGCAGCTATGAGTGACACTGCCAGTAGATACAAAGACAACCTAAAGAAATTTTTTACCGACATTCGCAATGATATAAAACCTAGATTTTTACCCATCATTCTTGTTAAAATAGCCCTCTATGACTTTATGATGAAGCATGATACCCATGATCTGCCAGCAGTGAGGGCGGCAGAAGATGCAGTGAGTAAGGAGTTACCAAACGTGGTGACCATTGATGCCTTAAAATTACCTATAAACTTTGACACACACGAAGGCTTTAACCTAGATCATGGTCATTTTAATACCACAACGGAAATTACTTTGGGTAAATGGTTGGCTGATACCTACCTCTCCCACTATGGTCATTTACTCTAA

mRNA sequence

ATGATATTTGGCACTTCCCTCTCAGGGGCTACTTCTCCTAAGAACATATTCATCCTCGCTGGTCAGAGTAATATGGCTGGTCGAGGTGGGGTTGAGAACAATCTATTGGGAAAGCTCGAGTGGGATGGATTGATCCCACCAGAATGTCAATCTGACCCATCCATCCTACGATTGAACCCTGCTCGCCAATGGGAGATAGCACGAGAGCCTCTCCATGAGGGGATCGACATCAACAAGACAGTTGGGATTGGTCCGGGAATGTCATTTGCTCACCAACTGCTAGCTAAAGCAGGCCCAAATGCAGGTGTTGTGGGCTTAGTTCCTTGTGCTAGAGGTGGCACTTTAATTGAACAATGGATTAAAAATCCTAGCAATCCTAATGCAACTTTTTACCAAAATTTCATTGAACGAATCAAAGCATCAGATAAAGATGGTGGAGTTGTGCGTGCTCTTTTCTGGTTTCAAGGTGAGAGTGATGCAGCTATGAGTGACACTGCCAGTAGATACAAAGACAACCTAAAGAAATTTTTTACCGACATTCGCAATGATATAAAACCTAGATTTTTACCCATCATTCTTGTTAAAATAGCCCTCTATGACTTTATGATGAAGCATGATACCCATGATCTGCCAGCAGTGAGGGCGGCAGAAGATGCAGTGAGTAAGGAGTTACCAAACGTGGTGACCATTGATGCCTTAAAATTACCTATAAACTTTGACACACACGAAGGCTTTAACCTAGATCATGGTCATTTTAATACCACAACGGAAATTACTTTGGGTAAATGGTTGGCTGATACCTACCTCTCCCACTATGGTCATTTACTCTAA

Coding sequence (CDS)

ATGATATTTGGCACTTCCCTCTCAGGGGCTACTTCTCCTAAGAACATATTCATCCTCGCTGGTCAGAGTAATATGGCTGGTCGAGGTGGGGTTGAGAACAATCTATTGGGAAAGCTCGAGTGGGATGGATTGATCCCACCAGAATGTCAATCTGACCCATCCATCCTACGATTGAACCCTGCTCGCCAATGGGAGATAGCACGAGAGCCTCTCCATGAGGGGATCGACATCAACAAGACAGTTGGGATTGGTCCGGGAATGTCATTTGCTCACCAACTGCTAGCTAAAGCAGGCCCAAATGCAGGTGTTGTGGGCTTAGTTCCTTGTGCTAGAGGTGGCACTTTAATTGAACAATGGATTAAAAATCCTAGCAATCCTAATGCAACTTTTTACCAAAATTTCATTGAACGAATCAAAGCATCAGATAAAGATGGTGGAGTTGTGCGTGCTCTTTTCTGGTTTCAAGGTGAGAGTGATGCAGCTATGAGTGACACTGCCAGTAGATACAAAGACAACCTAAAGAAATTTTTTACCGACATTCGCAATGATATAAAACCTAGATTTTTACCCATCATTCTTGTTAAAATAGCCCTCTATGACTTTATGATGAAGCATGATACCCATGATCTGCCAGCAGTGAGGGCGGCAGAAGATGCAGTGAGTAAGGAGTTACCAAACGTGGTGACCATTGATGCCTTAAAATTACCTATAAACTTTGACACACACGAAGGCTTTAACCTAGATCATGGTCATTTTAATACCACAACGGAAATTACTTTGGGTAAATGGTTGGCTGATACCTACCTCTCCCACTATGGTCATTTACTCTAA

Protein sequence

MIFGTSLSGATSPKNIFILAGQSNMAGRGGVENNLLGKLEWDGLIPPECQSDPSILRLNPARQWEIAREPLHEGIDINKTVGIGPGMSFAHQLLAKAGPNAGVVGLVPCARGGTLIEQWIKNPSNPNATFYQNFIERIKASDKDGGVVRALFWFQGESDAAMSDTASRYKDNLKKFFTDIRNDIKPRFLPIILVKIALYDFMMKHDTHDLPAVRAAEDAVSKELPNVVTIDALKLPINFDTHEGFNLDHGHFNTTTEITLGKWLADTYLSHYGHLL

Homology

[Close]

BLAST of Lsi08G014990 vs. ExPASy Swiss-Prot
Match: Q8L9J9 (Probable carbohydrate esterase At4g34215 OS=Arabidopsis thaliana OX=3702 GN=At4g34215 PE=1 SV=2)

HSP 1 Score: 183.3 bits (464), Expect = 3.6e-45
Identity = 104/261 (39.85%), Postives = 151/261 (57.85%), Query Frame = 0


Query: 13  PKNIFILAGQSNMAGRGGV-ENNLLGKLEWDGLIPPECQSDPSILRLNPARQWEIAREPL 72
           P  IFIL+GQSNMAGRGGV +++   +  WD ++PPEC  + SILRL+   +WE A EPL
Sbjct: 21  PNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLRWEEAHEPL 80

Query: 73  HEGIDINKTVGIGPGMSFAHQLLAKAGPNAGVVGLVPCARGGTLIEQWIKNPSNPNATFY 132
           H  ID  K  G+GPGM+FA+ +  +   ++ V+GLVPCA GGT I++W +      +  Y
Sbjct: 81  HVDIDTGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWER-----GSHLY 140

Query: 133 QNFIERIKASDKDGGVVRALFWFQGESDAAMSDTASRYKDNLKKFFTDIRNDIKPRFLPI 192
           +  ++R + S K GG ++A+ W+QGESD      A  Y +N+ +   ++R+D+    LPI
Sbjct: 141 ERMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHDLNLPSLPI 200

Query: 193 ILVKIALYDFMMKHDTHDLPAVRAAEDAVSKELPNVVTIDALKLPINFDTHEGFNLDHGH 252
           I V IA            +  VR A+  +  +L NVV +DA  LP+  D     NL   H
Sbjct: 201 IQVAIA-------SGGGYIDKVREAQ--LGLKLSNVVCVDAKGLPLKSD-----NL---H 259

Query: 253 FNTTTEITLGKWLADTYLSHY 273
             T  ++ LG  LA  YLS++
Sbjct: 261 LTTEAQVQLGLSLAQAYLSNF 259

[Close]

BLAST of Lsi08G014990 vs. ExPASy TrEMBL
Match: A0A0A0LNC5 (SASA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G356040 PE=4 SV=1)

HSP 1 Score: 493.4 bits (1269), Expect = 6.0e-136
Identity = 232/276 (84.06%), Postives = 254/276 (92.03%), Query Frame = 0


Query: 1   MIFGTSLSGATSPKNIFILAGQSNMAGRGGVENNLLGKLEWDGLIPPECQSDPSILRLNP 60
           M++G SLSGA SPKNIFILAGQSNMAGRGGVENN  G L+WDGL+PPECQ  PSILRLNP
Sbjct: 13  MLYGPSLSGAASPKNIFILAGQSNMAGRGGVENNAQGNLQWDGLVPPECQPQPSILRLNP 72

Query: 61  ARQWEIAREPLHEGIDINKTVGIGPGMSFAHQLLAKAGPNAGVVGLVPCARGGTLIEQWI 120
             QWEIAREPLH GIDI +T GIGPG++FAH+LL KAGPNAG VGLVPCARGGTLIEQWI
Sbjct: 73  GLQWEIAREPLHLGIDIKRTPGIGPGIAFAHELLVKAGPNAGAVGLVPCARGGTLIEQWI 132

Query: 121 KNPSNPNATFYQNFIERIKASDKDGGVVRALFWFQGESDAAMSDTASRYKDNLKKFFTDI 180
           KNPSNP+ATFYQNFIERIKASDKDGGVVRALFWFQGESDAAM+DTA RYKDNLKKFFTDI
Sbjct: 133 KNPSNPSATFYQNFIERIKASDKDGGVVRALFWFQGESDAAMNDTAIRYKDNLKKFFTDI 192

Query: 181 RNDIKPRFLPIILVKIALYDFMMKHDTHDLPAVRAAEDAVSKELPNVVTIDALKLPINFD 240
           R+DIKPRFLPII+VKIALYDF  +HDTH+LPAVR A++AVSKELP+VV ID+LKLPIN+ 
Sbjct: 193 RDDIKPRFLPIIVVKIALYDFFRQHDTHNLPAVREAKEAVSKELPDVVAIDSLKLPINYT 252

Query: 241 THEGFNLDHGHFNTTTEITLGKWLADTYLSHYGHLL 277
           T+EG NLDHGHFNTTTEITLGKWLA+TYLSH+G LL
Sbjct: 253 TNEGINLDHGHFNTTTEITLGKWLAETYLSHFGQLL 288

[Close]

BLAST of Lsi08G014990 vs. ExPASy TrEMBL
Match: A0A6J1KIR8 (probable carbohydrate esterase At4g34215 OS=Cucurbita maxima OX=3661 GN=LOC111496116 PE=4 SV=1)

HSP 1 Score: 483.0 bits (1242), Expect = 8.2e-133
Identity = 228/276 (82.61%), Postives = 248/276 (89.86%), Query Frame = 0


Query: 1   MIFGTSLSGATSPKNIFILAGQSNMAGRGGVENNLLGKLEWDGLIPPECQSDPSILRLNP 60
           ++F  SLS ATSP NIFILAGQSNMAGRGGVENN  GKLEWDG +P ECQSDPSILRLNP
Sbjct: 13  ILFHPSLSWATSPTNIFILAGQSNMAGRGGVENNQKGKLEWDGKVPLECQSDPSILRLNP 72

Query: 61  ARQWEIAREPLHEGIDINKTVGIGPGMSFAHQLLAKAGPNAGVVGLVPCARGGTLIEQWI 120
           ARQWEIA+EPLH GIDI KT GIGPG+ FAHQ  AKAG  AG+VGLVPCARGGTLIEQWI
Sbjct: 73  ARQWEIAQEPLHLGIDIGKTPGIGPGIPFAHQFKAKAGQKAGIVGLVPCARGGTLIEQWI 132

Query: 121 KNPSNPNATFYQNFIERIKASDKDGGVVRALFWFQGESDAAMSDTASRYKDNLKKFFTDI 180
           KNPSNP+ATFYQNFIERIK S+K+GGVVRALFW+QGESDAAMSDTA RYKDNLKKF TDI
Sbjct: 133 KNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMSDTAHRYKDNLKKFITDI 192

Query: 181 RNDIKPRFLPIILVKIALYDFMMKHDTHDLPAVRAAEDAVSKELPNVVTIDALKLPINFD 240
           RNDIKPRFLP+I+VKI++YDF MKHDTHDLPAVRAAEDAV KELP+++TID+ +LPINF 
Sbjct: 193 RNDIKPRFLPVIIVKISMYDFFMKHDTHDLPAVRAAEDAVQKELPDIITIDSWELPINFT 252

Query: 241 THEGFNLDHGHFNTTTEITLGKWLADTYLSHYGHLL 277
           T EGF LDHGHFNT TEI LGKWLADTYL+HY HLL
Sbjct: 253 TFEGFCLDHGHFNTATEIALGKWLADTYLAHYSHLL 288

[Close]

BLAST of Lsi08G014990 vs. ExPASy TrEMBL
Match: A0A5A7VGP3 (Putative carbohydrate esterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold37G00180 PE=4 SV=1)

HSP 1 Score: 481.5 bits (1238), Expect = 2.4e-132
Identity = 228/271 (84.13%), Postives = 244/271 (90.04%), Query Frame = 0


Query: 6   SLSGATSPKNIFILAGQSNMAGRGGVENNLLGKLEWDGLIPPECQSDPSILRLNPARQWE 65
           SLSGA SPKNIFILAGQSNMAGRGGVE +  G L WD L+PPEC+  PSILRLNP R+WE
Sbjct: 24  SLSGAASPKNIFILAGQSNMAGRGGVEKDQSGNLVWDRLVPPECEPQPSILRLNPEREWE 83

Query: 66  IAREPLHEGIDINKTVGIGPGMSFAHQLLAKAGPNAGVVGLVPCARGGTLIEQWIKNPSN 125
            AREPLH GIDIN+T GIGPGM FAH LLAKAGPNAGVVGLVPCARGGTLIEQWIKNPSN
Sbjct: 84  TAREPLHVGIDINRTAGIGPGMPFAHHLLAKAGPNAGVVGLVPCARGGTLIEQWIKNPSN 143

Query: 126 PNATFYQNFIERIKASDKDGGVVRALFWFQGESDAAMSDTASRYKDNLKKFFTDIRNDIK 185
           PNATFY+NFIERIKASDKDGGVVRALFWFQGESDAAMSDTA RYKDNLK+FFTDIRNDIK
Sbjct: 144 PNATFYKNFIERIKASDKDGGVVRALFWFQGESDAAMSDTAHRYKDNLKQFFTDIRNDIK 203

Query: 186 PRFLPIILVKIALYDFMMKHDTHDLPAVRAAEDAVSKELPNVVTIDALKLPINFDTHEGF 245
           PRFLPIIL KIA+YD  MKHDTHDL AVRAA++ VSKELP+++TIDAL+LPINF T+ GF
Sbjct: 204 PRFLPIILAKIAVYDPFMKHDTHDLAAVRAAQEEVSKELPDILTIDALQLPINFTTNAGF 263

Query: 246 NLDHGHFNTTTEITLGKWLADTYLSHYGHLL 277
           NLDH HFNT TEI +GKW ADTYLSHYGHLL
Sbjct: 264 NLDHAHFNTNTEIVVGKWFADTYLSHYGHLL 294

[Close]

BLAST of Lsi08G014990 vs. ExPASy TrEMBL
Match: A0A5A7V246 (Putative carbohydrate esterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00600 PE=4 SV=1)

HSP 1 Score: 478.4 bits (1230), Expect = 2.0e-131
Identity = 227/276 (82.25%), Postives = 251/276 (90.94%), Query Frame = 0


Query: 1   MIFGTSLSGATSPKNIFILAGQSNMAGRGGVENNLLGKLEWDGLIPPECQSDPSILRLNP 60
           M++G+ LSGA  PKNIFILAGQSNMAGRGGVE     KL WDGL+PPEC  +PSILRLNP
Sbjct: 1   MLYGSYLSGAVPPKNIFILAGQSNMAGRGGVE-MYNKKLTWDGLVPPECTPEPSILRLNP 60

Query: 61  ARQWEIAREPLHEGIDINKTVGIGPGMSFAHQLLAKAGPNAGVVGLVPCARGGTLIEQWI 120
            RQWE+AREPLH GIDIN+T GIGPG+ FA +LLAKAGP AG VGLVPCARGGTLI QW+
Sbjct: 61  DRQWEVAREPLHLGIDINRTPGIGPGIPFAKELLAKAGPKAGAVGLVPCARGGTLIGQWL 120

Query: 121 KNPSNPNATFYQNFIERIKASDKDGGVVRALFWFQGESDAAMSDTASRYKDNLKKFFTDI 180
           KNPSNP+ATFYQNFIERI+ SDKDGGVVRALFWFQGESDAAM+DTA RYKDNL KFFTDI
Sbjct: 121 KNPSNPSATFYQNFIERIRTSDKDGGVVRALFWFQGESDAAMNDTAMRYKDNLMKFFTDI 180

Query: 181 RNDIKPRFLPIILVKIALYDFMMKHDTHDLPAVRAAEDAVSKELPNVVTIDALKLPINFD 240
           RNDIKPRFLPI++VKIALYDF MKHDTH+LPAVRAA+DAV+KELP+VVTIDAL+LPINF 
Sbjct: 181 RNDIKPRFLPIVVVKIALYDFFMKHDTHNLPAVRAAQDAVAKELPDVVTIDALELPINFT 240

Query: 241 THEGFNLDHGHFNTTTEITLGKWLADTYLSHYGHLL 277
           T+EG NLDHGHFNT+TEITLGKWLA+TYLSH+GHLL
Sbjct: 241 TNEGLNLDHGHFNTSTEITLGKWLANTYLSHFGHLL 275

[Close]

BLAST of Lsi08G014990 vs. ExPASy TrEMBL
Match: A0A6J1BQ38 (probable carbohydrate esterase At4g34215 OS=Momordica charantia OX=3673 GN=LOC111004778 PE=4 SV=1)

HSP 1 Score: 470.7 bits (1210), Expect = 4.2e-129
Identity = 226/276 (81.88%), Postives = 243/276 (88.04%), Query Frame = 0


Query: 1   MIFGTSLSGATSPKNIFILAGQSNMAGRGGVENNLLGKLEWDGLIPPECQSDPSILRLNP 60
           M+FG SLSGATSPKNIFILAGQSNMAGRGGVE N  G LEWDG +PPE Q DPSILRLNP
Sbjct: 13  MLFGPSLSGATSPKNIFILAGQSNMAGRGGVEKNRTGDLEWDGYVPPESQPDPSILRLNP 72

Query: 61  ARQWEIAREPLHEGIDINKTVGIGPGMSFAHQLLAKAGPNAGVVGLVPCARGGTLIEQWI 120
            RQWE+AREP+H GIDI KTVG+GP ++FAHQL AK G   G VGLVPCARGGTLIEQW+
Sbjct: 73  ERQWEVAREPVHRGIDIGKTVGVGPAIAFAHQLQAKGGSKVGSVGLVPCARGGTLIEQWV 132

Query: 121 KNPSNPNATFYQNFIERIKASDKDGGVVRALFWFQGESDAAMSDTASRYKDNLKKFFTDI 180
           KNPSNPNATFY+NFIERI+ASD++GGVVRALFW QGESDAA SDTA RYK+NLKKFFTDI
Sbjct: 133 KNPSNPNATFYKNFIERIQASDREGGVVRALFWLQGESDAASSDTAERYKNNLKKFFTDI 192

Query: 181 RNDIKPRFLPIILVKIALYDFMMKHDTHDLPAVRAAEDAVSKELPNVVTIDALKLPINFD 240
           RNDIKPR LPIILVKIA+YD  MKHDTHDLPAVRAAEDAV +ELPNVVTIDALKL +N  
Sbjct: 193 RNDIKPRVLPIILVKIAVYDTFMKHDTHDLPAVRAAEDAVQRELPNVVTIDALKL-VNTT 252

Query: 241 THEGFNLDHGHFNTTTEITLGKWLADTYLSHYGHLL 277
           T EGFNLD GHFN  TEI LGKWLADTYLS+YGHLL
Sbjct: 253 TAEGFNLDRGHFNIKTEIALGKWLADTYLSNYGHLL 287

[Close]

BLAST of Lsi08G014990 vs. NCBI nr
Match: XP_038886442.1 (probable carbohydrate esterase At4g34215 [Benincasa hispida])

HSP 1 Score: 525.4 bits (1352), Expect = 3.0e-145
Identity = 249/276 (90.22%), Postives = 262/276 (94.93%), Query Frame = 0


Query: 1   MIFGTSLSGATSPKNIFILAGQSNMAGRGGVENNLLGKLEWDGLIPPECQSDPSILRLNP 60
           M+FG+SLS A SP NIFILAGQSNMAGRGGVENN + +LEWDGLIPPECQSDPSILRLNP
Sbjct: 13  MLFGSSLSRAASPNNIFILAGQSNMAGRGGVENNQVRELEWDGLIPPECQSDPSILRLNP 72

Query: 61  ARQWEIAREPLHEGIDINKTVGIGPGMSFAHQLLAKAGPNAGVVGLVPCARGGTLIEQWI 120
           A QWEIAREPLHEGIDINKTVGIGPGM FAHQLL K GP AG VGLVPCARGGT+IEQWI
Sbjct: 73  ALQWEIAREPLHEGIDINKTVGIGPGMPFAHQLLTKVGPRAGTVGLVPCARGGTIIEQWI 132

Query: 121 KNPSNPNATFYQNFIERIKASDKDGGVVRALFWFQGESDAAMSDTASRYKDNLKKFFTDI 180
           KNPSNP+ATFY+NFIERIKASDK+GGVVRALFWFQGESDAAMSDTA+RYKDNLK FFTDI
Sbjct: 133 KNPSNPDATFYKNFIERIKASDKEGGVVRALFWFQGESDAAMSDTANRYKDNLKNFFTDI 192

Query: 181 RNDIKPRFLPIILVKIALYDFMMKHDTHDLPAVRAAEDAVSKELPNVVTIDALKLPINFD 240
           RNDIKPRFLPIILVKIALYDFMMKHDTHDLPAVRAA+DAVSKELP++VTIDALKLPIN D
Sbjct: 193 RNDIKPRFLPIILVKIALYDFMMKHDTHDLPAVRAAQDAVSKELPDIVTIDALKLPINVD 252

Query: 241 THEGFNLDHGHFNTTTEITLGKWLADTYLSHYGHLL 277
           THEGFN DHGHFNTTT+ITLGKWLADTYLSHYGHLL
Sbjct: 253 THEGFNQDHGHFNTTTQITLGKWLADTYLSHYGHLL 288

[Close]

BLAST of Lsi08G014990 vs. NCBI nr
Match: XP_038886575.1 (probable carbohydrate esterase At4g34215 [Benincasa hispida])

HSP 1 Score: 521.2 bits (1341), Expect = 5.6e-144
Identity = 247/276 (89.49%), Postives = 263/276 (95.29%), Query Frame = 0


Query: 1   MIFGTSLSGATSPKNIFILAGQSNMAGRGGVENNLLGKLEWDGLIPPECQSDPSILRLNP 60
           ++FG+SLS A SPKNIFILAGQSNMAGRGGVENN +GKLEW+ LIPPECQSD SILRLNP
Sbjct: 13  LLFGSSLSRAASPKNIFILAGQSNMAGRGGVENNQVGKLEWNRLIPPECQSDTSILRLNP 72

Query: 61  ARQWEIAREPLHEGIDINKTVGIGPGMSFAHQLLAKAGPNAGVVGLVPCARGGTLIEQWI 120
           A QWE+AREPLHEGIDINKTVGIGPGM FAHQLLAK GP AG+VGLVPCA+GGT+IEQWI
Sbjct: 73  ALQWEMAREPLHEGIDINKTVGIGPGMPFAHQLLAKVGPKAGIVGLVPCAKGGTIIEQWI 132

Query: 121 KNPSNPNATFYQNFIERIKASDKDGGVVRALFWFQGESDAAMSDTASRYKDNLKKFFTDI 180
           KNPSNP+ATFY++FIERIKASDK+GGVVRALFWFQGESDAAM+DTASRYKDNLK FFTDI
Sbjct: 133 KNPSNPDATFYKSFIERIKASDKEGGVVRALFWFQGESDAAMNDTASRYKDNLKNFFTDI 192

Query: 181 RNDIKPRFLPIILVKIALYDFMMKHDTHDLPAVRAAEDAVSKELPNVVTIDALKLPINFD 240
           RNDIKPRFLPIILVKIALYDFMMKHDTHDLP VRAA+DAVSKELP+VVTIDALKLPIN D
Sbjct: 193 RNDIKPRFLPIILVKIALYDFMMKHDTHDLPVVRAAQDAVSKELPDVVTIDALKLPINVD 252

Query: 241 THEGFNLDHGHFNTTTEITLGKWLADTYLSHYGHLL 277
           THEGFN DHGHFNTTTEITLGKWLADTYLSHYGHLL
Sbjct: 253 THEGFNQDHGHFNTTTEITLGKWLADTYLSHYGHLL 288

[Close]

BLAST of Lsi08G014990 vs. NCBI nr
Match: KAE8646868.1 (hypothetical protein Csa_020851 [Cucumis sativus])

HSP 1 Score: 501.5 bits (1290), Expect = 4.6e-138
Identity = 237/276 (85.87%), Postives = 255/276 (92.39%), Query Frame = 0


Query: 1   MIFGTSLSGATSPKNIFILAGQSNMAGRGGVENNLLGKLEWDGLIPPECQSDPSILRLNP 60
           M++   LSGA SPKNIFI AGQSNMAGRGGVENN  G L WDGL+PPECQS+PSILRLNP
Sbjct: 13  MLYSPCLSGAISPKNIFIFAGQSNMAGRGGVENNNKGNLMWDGLVPPECQSEPSILRLNP 72

Query: 61  ARQWEIAREPLHEGIDINKTVGIGPGMSFAHQLLAKAGPNAGVVGLVPCARGGTLIEQWI 120
            RQWEIAREPLH GIDIN+T GIGPGM FAH+LLAK GPNAG VGLVPCARGGTLI QW+
Sbjct: 73  DRQWEIAREPLHLGIDINRTPGIGPGMPFAHELLAKVGPNAGAVGLVPCARGGTLIGQWV 132

Query: 121 KNPSNPNATFYQNFIERIKASDKDGGVVRALFWFQGESDAAMSDTASRYKDNLKKFFTDI 180
           KNPSNP+ATFYQNFIERIKASDKDGGVVRALFWFQGESDAAM+DTA RYKDNLKKFFTDI
Sbjct: 133 KNPSNPSATFYQNFIERIKASDKDGGVVRALFWFQGESDAAMNDTAIRYKDNLKKFFTDI 192

Query: 181 RNDIKPRFLPIILVKIALYDFMMKHDTHDLPAVRAAEDAVSKELPNVVTIDALKLPINFD 240
           RNDIKPRFLPII+VKIALYDFMM+HDTH+LPAVR A+DAVSKELP+VV ID+L+LPIN  
Sbjct: 193 RNDIKPRFLPIIVVKIALYDFMMQHDTHNLPAVREAQDAVSKELPDVVAIDSLELPINLT 252

Query: 241 THEGFNLDHGHFNTTTEITLGKWLADTYLSHYGHLL 277
           T+EGFNLDHGHFNTTTEITLGKWLA+TYLSHYGHLL
Sbjct: 253 TNEGFNLDHGHFNTTTEITLGKWLANTYLSHYGHLL 288

[Close]

BLAST of Lsi08G014990 vs. NCBI nr
Match: XP_031736508.1 (probable carbohydrate esterase At4g34215 [Cucumis sativus] >XP_031736509.1 probable carbohydrate esterase At4g34215 [Cucumis sativus] >XP_031736510.1 probable carbohydrate esterase At4g34215 [Cucumis sativus])

HSP 1 Score: 496.1 bits (1276), Expect = 1.9e-136
Identity = 233/276 (84.42%), Postives = 255/276 (92.39%), Query Frame = 0


Query: 1   MIFGTSLSGATSPKNIFILAGQSNMAGRGGVENNLLGKLEWDGLIPPECQSDPSILRLNP 60
           M++G SLSGA SPKNIFILAGQSNMAGRGGVENN  G L+WDGL+PPECQ  PSILRLNP
Sbjct: 13  MLYGPSLSGAASPKNIFILAGQSNMAGRGGVENNAQGNLQWDGLVPPECQPQPSILRLNP 72

Query: 61  ARQWEIAREPLHEGIDINKTVGIGPGMSFAHQLLAKAGPNAGVVGLVPCARGGTLIEQWI 120
             QWEIAREPLH GIDIN+T GIGPG++FAH+LL KAGPNAG VGLVPCARGGTLIEQWI
Sbjct: 73  GLQWEIAREPLHLGIDINRTPGIGPGIAFAHELLVKAGPNAGAVGLVPCARGGTLIEQWI 132

Query: 121 KNPSNPNATFYQNFIERIKASDKDGGVVRALFWFQGESDAAMSDTASRYKDNLKKFFTDI 180
           KNPSNP+ATFYQNFIERIKASDKDGGVVRALFWFQGESDAAM+DTA RYKDNLKKFFTDI
Sbjct: 133 KNPSNPSATFYQNFIERIKASDKDGGVVRALFWFQGESDAAMNDTAIRYKDNLKKFFTDI 192

Query: 181 RNDIKPRFLPIILVKIALYDFMMKHDTHDLPAVRAAEDAVSKELPNVVTIDALKLPINFD 240
           R+DIKPRFLPII+VKIALYDF  +HDTH+LPAVR A++AVSKELP+VV ID+LKLPIN+ 
Sbjct: 193 RDDIKPRFLPIIVVKIALYDFFRQHDTHNLPAVREAQEAVSKELPDVVAIDSLKLPINYT 252

Query: 241 THEGFNLDHGHFNTTTEITLGKWLADTYLSHYGHLL 277
           T+EG NLDHGHFNTTTEITLGKWLA+TYLSH+G LL
Sbjct: 253 TNEGINLDHGHFNTTTEITLGKWLAETYLSHFGQLL 288

[Close]

BLAST of Lsi08G014990 vs. NCBI nr
Match: KAE8652070.1 (hypothetical protein Csa_018719 [Cucumis sativus])

HSP 1 Score: 495.7 bits (1275), Expect = 2.5e-136
Identity = 232/276 (84.06%), Postives = 256/276 (92.75%), Query Frame = 0


Query: 1   MIFGTSLSGATSPKNIFILAGQSNMAGRGGVENNLLGKLEWDGLIPPECQSDPSILRLNP 60
           M++G SLSGA SPKNIFILAGQSNMAGRGGVENN  GKL+WDGL+PPECQ  PSILRLNP
Sbjct: 13  MLYGPSLSGAVSPKNIFILAGQSNMAGRGGVENNAQGKLQWDGLVPPECQPQPSILRLNP 72

Query: 61  ARQWEIAREPLHEGIDINKTVGIGPGMSFAHQLLAKAGPNAGVVGLVPCARGGTLIEQWI 120
            RQWEIAREPLH GIDI +T GIGPG++FAH+LLAKAGPNAG VGLVPCARGGTLIE+W+
Sbjct: 73  DRQWEIAREPLHLGIDIKRTPGIGPGIAFAHELLAKAGPNAGAVGLVPCARGGTLIEEWV 132

Query: 121 KNPSNPNATFYQNFIERIKASDKDGGVVRALFWFQGESDAAMSDTASRYKDNLKKFFTDI 180
           KNPSNP+ATFYQNFIERIKASDKDGGVVRALFWFQGESDAAM+DTA RYKDNLKKFFTDI
Sbjct: 133 KNPSNPSATFYQNFIERIKASDKDGGVVRALFWFQGESDAAMNDTAIRYKDNLKKFFTDI 192

Query: 181 RNDIKPRFLPIILVKIALYDFMMKHDTHDLPAVRAAEDAVSKELPNVVTIDALKLPINFD 240
           R+DIKPR+LPII+VKIALYDF   HDTH+LPAVR A++AVSKELP+VV ID+LKLPIN+ 
Sbjct: 193 RDDIKPRYLPIIVVKIALYDFFRPHDTHNLPAVREAQEAVSKELPDVVAIDSLKLPINYT 252

Query: 241 THEGFNLDHGHFNTTTEITLGKWLADTYLSHYGHLL 277
           T+EG NLDHGHFNTTTEITLGKWLA+TYLSH+G LL
Sbjct: 253 TNEGINLDHGHFNTTTEITLGKWLAETYLSHFGQLL 288

[Close]

BLAST of Lsi08G014990 vs. TAIR 10
Match: AT3G53010.1 (Domain of unknown function (DUF303) )

HSP 1 Score: 195.3 bits (495), Expect = 6.5e-50
Identity = 115/259 (44.40%), Postives = 156/259 (60.23%), Query Frame = 0


Query: 15  NIFILAGQSNMAGRGGVENN-LLGKLEWDGLIPPECQSDPSILRLNPARQWEIAREPLHE 74
           +IFILAGQSNMAGRGGV N+       WDG+IPPEC+S+PSILRL    +W+ A+EPLH 
Sbjct: 30  SIFILAGQSNMAGRGGVYNDTATNTTVWDGVIPPECRSNPSILRLTSKLEWKEAKEPLHV 89

Query: 75  GIDINKTVGIGPGMSFAHQLLAKAGPNAGVVGLVPCARGGTLIEQWIKNPSNPNATFYQN 134
            IDINKT G+GPGM FA++++ + G     VGLVPC+ GGT + QW K         Y+ 
Sbjct: 90  DIDINKTNGVGPGMPFANRVVNRFGQ----VGLVPCSIGGTKLSQWQK-----GEFLYEE 149

Query: 135 FIERIKA--SDKDGGVVRALFWFQGESDAAMSDTASRYKDNLKKFFTDIRNDIKPRFLPI 194
            ++R KA  +   GG  RA+ W+QGESD      AS YK  L KFF+D+RND++   LPI
Sbjct: 150 TVKRAKAAMASGGGGSYRAVLWYQGESDTVDMVDASVYKKRLVKFFSDLRNDLQHPNLPI 209

Query: 195 ILVKIALYDFMMKHDTHDLPAVRAAEDAVSKELPNVVTIDALKLPINFDTHEGFNLDHGH 254
           I V +A            L AVR A+  +  +L NV  +DA  LP+          D  H
Sbjct: 210 IQVALA------TGAGPYLDAVRKAQ--LKTDLENVYCVDARGLPL--------EPDGLH 263

Query: 255 FNTTTEITLGKWLADTYLS 271
             T++++ LG  +A+++L+
Sbjct: 270 LTTSSQVQLGHMIAESFLA 263

[Close]

BLAST of Lsi08G014990 vs. TAIR 10
Match: AT4G34215.1 (Domain of unknown function (DUF303) )

HSP 1 Score: 183.3 bits (464), Expect = 2.6e-46
Identity = 104/261 (39.85%), Postives = 151/261 (57.85%), Query Frame = 0


Query: 13  PKNIFILAGQSNMAGRGGV-ENNLLGKLEWDGLIPPECQSDPSILRLNPARQWEIAREPL 72
           P  IFIL+GQSNMAGRGGV +++   +  WD ++PPEC  + SILRL+   +WE A EPL
Sbjct: 21  PNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLRWEEAHEPL 80

Query: 73  HEGIDINKTVGIGPGMSFAHQLLAKAGPNAGVVGLVPCARGGTLIEQWIKNPSNPNATFY 132
           H  ID  K  G+GPGM+FA+ +  +   ++ V+GLVPCA GGT I++W +      +  Y
Sbjct: 81  HVDIDTGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWER-----GSHLY 140

Query: 133 QNFIERIKASDKDGGVVRALFWFQGESDAAMSDTASRYKDNLKKFFTDIRNDIKPRFLPI 192
           +  ++R + S K GG ++A+ W+QGESD      A  Y +N+ +   ++R+D+    LPI
Sbjct: 141 ERMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHDLNLPSLPI 200

Query: 193 ILVKIALYDFMMKHDTHDLPAVRAAEDAVSKELPNVVTIDALKLPINFDTHEGFNLDHGH 252
           I V IA            +  VR A+  +  +L NVV +DA  LP+  D     NL   H
Sbjct: 201 IQVAIA-------SGGGYIDKVREAQ--LGLKLSNVVCVDAKGLPLKSD-----NL---H 259

Query: 253 FNTTTEITLGKWLADTYLSHY 273
             T  ++ LG  LA  YLS++
Sbjct: 261 LTTEAQVQLGLSLAQAYLSNF 259

[Close]

BLAST of Lsi08G014990 vs. TAIR 10
Match: AT4G34215.2 (Domain of unknown function (DUF303) )

HSP 1 Score: 183.3 bits (464), Expect = 2.6e-46
Identity = 104/261 (39.85%), Postives = 151/261 (57.85%), Query Frame = 0


Query: 13  PKNIFILAGQSNMAGRGGV-ENNLLGKLEWDGLIPPECQSDPSILRLNPARQWEIAREPL 72
           P  IFIL+GQSNMAGRGGV +++   +  WD ++PPEC  + SILRL+   +WE A EPL
Sbjct: 21  PNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLRWEEAHEPL 80

Query: 73  HEGIDINKTVGIGPGMSFAHQLLAKAGPNAGVVGLVPCARGGTLIEQWIKNPSNPNATFY 132
           H  ID  K  G+GPGM+FA+ +  +   ++ V+GLVPCA GGT I++W +      +  Y
Sbjct: 81  HVDIDTGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWER-----GSHLY 140

Query: 133 QNFIERIKASDKDGGVVRALFWFQGESDAAMSDTASRYKDNLKKFFTDIRNDIKPRFLPI 192
           +  ++R + S K GG ++A+ W+QGESD      A  Y +N+ +   ++R+D+    LPI
Sbjct: 141 ERMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHDLNLPSLPI 200

Query: 193 ILVKIALYDFMMKHDTHDLPAVRAAEDAVSKELPNVVTIDALKLPINFDTHEGFNLDHGH 252
           I V IA            +  VR A+  +  +L NVV +DA  LP+  D     NL   H
Sbjct: 201 IQVAIA-------SGGGYIDKVREAQ--LGLKLSNVVCVDAKGLPLKSD-----NL---H 259

Query: 253 FNTTTEITLGKWLADTYLSHY 273
             T  ++ LG  LA  YLS++
Sbjct: 261 LTTEAQVQLGLSLAQAYLSNF 259

The following BLAST results are available for this feature:

BLAST of Lsi08G014990 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L9J9	3.6e-45	39.85	Probable carbohydrate esterase At4g34215 OS=Arabidopsis thaliana OX=3702 GN=At4g...	[more]

BLAST of Lsi08G014990 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LNC5	6.0e-136	84.06	SASA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G356040 PE=4 S...	[more]
A0A6J1KIR8	8.2e-133	82.61	probable carbohydrate esterase At4g34215 OS=Cucurbita maxima OX=3661 GN=LOC11149...	[more]
A0A5A7VGP3	2.4e-132	84.13	Putative carbohydrate esterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A5A7V246	2.0e-131	82.25	Putative carbohydrate esterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s...	[more]
A0A6J1BQ38	4.2e-129	81.88	probable carbohydrate esterase At4g34215 OS=Momordica charantia OX=3673 GN=LOC11...	[more]

BLAST of Lsi08G014990 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038886442.1	3.0e-145	90.22	probable carbohydrate esterase At4g34215 [Benincasa hispida]	[more]
XP_038886575.1	5.6e-144	89.49	probable carbohydrate esterase At4g34215 [Benincasa hispida]	[more]
KAE8646868.1	4.6e-138	85.87	hypothetical protein Csa_020851 [Cucumis sativus]	[more]
XP_031736508.1	1.9e-136	84.42	probable carbohydrate esterase At4g34215 [Cucumis sativus] >XP_031736509.1 proba...	[more]
KAE8652070.1	2.5e-136	84.06	hypothetical protein Csa_018719 [Cucumis sativus]	[more]

BLAST of Lsi08G014990 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G53010.1	6.5e-50	44.40	Domain of unknown function (DUF303)	[more]
AT4G34215.1	2.6e-46	39.85	Domain of unknown function (DUF303)	[more]
AT4G34215.2	2.6e-46	39.85	Domain of unknown function (DUF303)	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005181	Sialate O-acetylesterase domain	PFAM	PF03629	SASA	coord: 14..269 e-value: 1.9E-70 score: 237.0
IPR036514	SGNH hydrolase superfamily	GENE3D	3.40.50.1110	SGNH hydrolase	coord: 12..273 e-value: 8.4E-57 score: 194.8
None	No IPR available	PANTHER	PTHR31988	ESTERASE, PUTATIVE (DUF303)-RELATED	coord: 11..272
None	No IPR available	PANTHER	PTHR31988:SF19	BNACNNG62850D PROTEIN	coord: 11..272
None	No IPR available	SUPERFAMILY	52266	SGNH hydrolase	coord: 15..273

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi08G014990.1	Lsi08G014990.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity