Lsi08G014760 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi08G014760 vs. ExPASy Swiss-Prot
Match: O04660 (Glutamate receptor 2.1 OS=Arabidopsis thaliana OX=3702 GN=GLR2.1 PE=2 SV=2)

HSP 1 Score: 258.8 bits (660), Expect = 2.0e-67
Identity = 213/743 (28.67%), Postives = 360/743 (48.45%), Query Frame = 0

Query: 4   LDLISIKEVSTILGAFTLQEMQLMSEINKTFIDIPIISLPVAASIPPNGLFPPPSFIQMA 63
           LDLI+ KEV  ILG +T  + Q M E+ +    +PI++   +A+ P         F +  
Sbjct: 89  LDLITNKEVKAILGPWTSMQAQFMIEMGQK-SQVPIVT--YSATSPSLASIRSQYFFRAT 148

Query: 64  HHITFHMQCTAALVGHFQWHKVTVIYEHTNDMSFNMEALTLLSNQLGVFHAEIEQISSFS 123
           +  +  +     ++  F W +V  +Y    D +F    +  L++ L   +  I   +  S
Sbjct: 149 YDDSSQVHAIKEIIKLFGWREVAPVYV---DDTFGEGIMPRLTDVLQEINVRIPYRTVIS 208

Query: 124 SSYTEAMIEEKLKNLVGHERNKVFIVVQFSIELAKLLFHKANKMKMMENGFVWIVGDEIS 183
            + T+  I  +L  ++     +VF+V    + LA   F KA ++ +M+ G+VWI+ + I+
Sbjct: 209 PNATDDEISVELLRMMTLP-TRVFVVHLVEL-LASRFFAKATEIGLMKQGYVWILTNTIT 268

Query: 184 SHLDSLDSSTFNDMQGVIGFRTYFDHNKDSFKKFRTKFLRKYVLEYHHQEEEMKNGEPSI 243
             L  ++ +    MQGV+G +TY   +K+  + FR+++ +++ +            + ++
Sbjct: 269 DVLSIMNETEIETMQGVLGVKTYVPRSKE-LENFRSRWTKRFPI-----------SDLNV 328

Query: 244 FALRAYDASWAVALA-------------------MNKLQGNFTNK---QLLKEILGSEFE 303
           + L AYDA+ A+ALA                   +++LQG   ++   +LL+ +    F+
Sbjct: 329 YGLWAYDATTALALAIEEAGTSNLTFVKMDAKRNVSELQGLGVSQYGPKLLQTLSRVRFQ 388

Query: 304 GLSGKIGFKNGALKEPPTFEIIYVVGKSYKEMGFWRQKVGFFSMIENDEEISSIIIDERR 363
           GL+G   F NG L +P  FEI+ V G+  + +GFW ++ G F  ++           +  
Sbjct: 389 GLAGDFQFINGEL-QPSVFEIVNVNGQGGRTIGFWMKEYGLFKNVD----------QKPA 448

Query: 364 SRSNNNGVLELPRFIFLEGNAKTGLIKGRINVDNSDSRVIGRTLRIGVPANNTFREFVKV 423
           S++  +   +  R I   G+  T + KG     N      G+ L+IGVP NNTF++FVK 
Sbjct: 449 SKTTFSSWQDRLRPIIWPGDT-TSVPKGWEIPTN------GKRLQIGVPVNNTFQQFVKA 508

Query: 424 SYNHI-NGMYISGFSITVFEAVAKNLPYPLSYQLVPF-NGSYDGLVKQVYEKDLDAAVGD 483
           + + I N    SGFSI  FEAV + +PY +SY  +PF +G YD LV QVY    DA V D
Sbjct: 509 TRDPITNSTIFSGFSIDYFEAVIQAIPYDISYDFIPFQDGGYDALVYQVYLGKYDAVVAD 568

Query: 484 IGIFADRFQYVDFTEPYLVSGLLLIVKEETENWKEIWVFMKTFTTTMWLILPFSHIFIIS 543
             I ++R  YVDF+ PY  SG+ L+V  +    +   +F+   T  +WLI   S   I  
Sbjct: 569 TTISSNRSMYVDFSLPYTPSGVGLVVPVKDSVRRSSTIFLMPLTLALWLISLLSFFIIGL 628

Query: 544 IVWLVKEE-SEDLSGFGE-----MLWFSVTVIFYTQRKEVKGFLARLVLGTWLFVILVVT 603
           +VW+++   + D  G G+     + WFS +++ +  R+ V  F AR+V+  W F++LV+T
Sbjct: 629 VVWVLEHRVNPDFDGPGQYQLSTIFWFSFSIMVFAPRERVLSFWARVVVIIWYFLVLVLT 688

Query: 604 SSFTASLTSMMTVSRFAPSVVDIETIRQTNATVGCNFHSFIIRYLNDVLHIPSANIKTLV 663
            S+TASL S++T     P+V +I ++     +VG    SFI+  L D     ++ +    
Sbjct: 689 QSYTASLASLLTTQHLHPTVTNINSLLAKGESVGYQ-SSFILGRLRDSGFSEASLVS--Y 748

Query: 664 GIDDY-----PKAFDNGEIQAAFFITPHAK------------------------AFPKGS 688
           G  ++      K    G + A     P+ +                         FP GS
Sbjct: 749 GSPEHCDALLSKGQAEGGVSAVLMEVPYVRIFLGQYCNKYKMVQTPFKVDGLGFVFPIGS 790

BLAST of Lsi08G014760 vs. ExPASy Swiss-Prot
Match: Q9LFN5 (Glutamate receptor 2.5 OS=Arabidopsis thaliana OX=3702 GN=GLR2.5 PE=2 SV=2)

HSP 1 Score: 252.3 bits (643), Expect = 1.9e-65
Identity = 216/790 (27.34%), Postives = 361/790 (45.70%), Query Frame = 0

Query: 2   SPLDLISIKEVSTILGAFTLQEMQLMSEINKTFIDIPIISLPVAASIPPNGLFPPPSFIQ 61
           S L LI  +EV  I+G  T  +   +  +      +PIIS   +A+ P       P FI+
Sbjct: 92  SALYLIKKREVVAIIGPGTSMQAPFLINLGNQ-SKVPIISF--SATSPLLDSLRSPYFIR 151

Query: 62  MAHHITFHMQCTAALVGHFQWHKVTVIYEHTNDMSFNMEALTLLSNQLGVFHAEIEQISS 121
             H  +  +Q  +A++  F+W +V  IY    D  F    L  L +     +  I   S+
Sbjct: 152 ATHDDSSQVQAISAIIESFRWREVVPIYV---DNEFGEGILPNLVDAFQEINVRIRYRSA 211

Query: 122 FSSSYTEAMIEEKLKNLVGHERNKVFIVVQFSIELAKLLFHKANKMKMMENGFVWIVGDE 181
            S  Y++  I+++L  L+     +VFIV     +L   LF  A ++ M+  G+VWIV + 
Sbjct: 212 ISLHYSDDQIKKELYKLMTMP-TRVFIVHMLP-DLGSRLFSIAKEIDMLSKGYVWIVTNG 271

Query: 182 ISSHLDSLDSSTFNDMQGVIGFRTYFDHNKDSFKKFRTKFLRKYVLEYHHQEEEMKNGEP 241
           I+  +  +  S+  +M GV+G +TYF  +K+       ++ +++        EE+ N   
Sbjct: 272 IADLMSIMGESSLVNMHGVLGVKTYFAKSKE-LLHLEARWQKRF------GGEELNN--- 331

Query: 242 SIFALRAYDASWAVALAMNKLQG-----NFTNKQLLKEILGSE----------------- 301
             FA  AYDA+ A+A+++ +++      N T +   ++ +G++                 
Sbjct: 332 --FACWAYDAATALAMSVEEIRHVNMSFNTTKEDTSRDDIGTDLDELGVALSGPKLLDAL 391

Query: 302 ----FEGLSGKIGFKNGALKEPPTFEIIYVVGKSYKEMGFWRQKVGFFSMIENDEEISSI 361
               F+G++G+   KNG L E  TF+II +     + +GFW+ KVG    +  D+   S 
Sbjct: 392 STVSFKGVAGRFQLKNGKL-EATTFKIINIEESGERTVGFWKSKVGLVKSLRVDKVSHS- 451

Query: 362 IIDERRSRSN--NNGVLELPRFIFLEGNAKTGLIKGRINVDNSDSRVIGRTLRIGVPANN 421
               RR R        + +P+      NAK                     LRI VP  +
Sbjct: 452 ---SRRLRPIIWPGDTIFVPKGWEFPTNAK--------------------KLRIAVPKKD 511

Query: 422 TFREFVKVSYN-HINGMYISGFSITVFEAVAKNLPYPLSYQLVPFN-------GSYDGLV 481
            F  FV+V+ + + N   ++GF I VF  V   +PY +SY+ +PF+       GSYD +V
Sbjct: 512 GFNNFVEVTKDENTNVPTVTGFCIDVFNTVMSQMPYAVSYEYIPFDTPDGKPRGSYDEMV 571

Query: 482 KQVYEKDLDAAVGDIGIFADRFQYVDFTEPYLVSGLLLIVKEETENWKEIWVFMKTFTTT 541
             V+  + D AVGD  I A+R  YVDF  PY  +G++ +V  +    K  WVF+K  T  
Sbjct: 572 YNVFLGEFDGAVGDTTILANRSHYVDFALPYSETGIVFLVPVKDGKEKGEWVFLKPLTKE 631

Query: 542 MWLILPFSHIFIISIVWLVKEESED-------LSGFGEMLWFSVTVIFYTQRKEVKGFLA 601
           +WL+   S ++I  +VW+ + ++++       +     + +FS + +F+  R+  + F  
Sbjct: 632 LWLVTAASFLYIGIMVWIFEYQADEEFREQMIIDKISSVFYFSFSTLFFAHRRPSESFFT 691

Query: 602 RLVLGTWLFVILVVTSSFTASLTSMMTVSRFAPSVVDIETIRQTNATVGCNFHSFIIRYL 661
           R+++  W FV+L++T S+TA+LTSM+TV    P+V  ++ +R++   +G    SF    L
Sbjct: 692 RVLVVVWCFVLLILTQSYTATLTSMLTVQELRPTVRHMDDLRKSGVNIGYQTGSFTFERL 751

Query: 662 NDVLHIPSANIKTLVGIDDYPKAF----DNGEIQAAF------------------FITPH 720
              +    + +KT    ++  + F     NG I AAF                   I P 
Sbjct: 752 KQ-MRFDESRLKTYNSPEEMRELFLHKSSNGGIDAAFDEVAYIKLFMAKYCSEYSIIEPT 811

BLAST of Lsi08G014760 vs. ExPASy Swiss-Prot
Match: Q9SHV1 (Glutamate receptor 2.2 OS=Arabidopsis thaliana OX=3702 GN=GLR2.2 PE=2 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 1.0e-63
Identity = 214/747 (28.65%), Postives = 354/747 (47.39%), Query Frame = 0

Query: 4   LDLISIKEVSTILGAFTLQEMQLMSEINKTFIDIPIISLPVAASIPPNGLFPPPSFIQMA 63
           +DLI  K+V  ILG +T  +   + EI +    +P++S   +A+ P       P F +  
Sbjct: 89  IDLIKNKQVKAILGPWTSMQAHFLIEIGQK-SRVPVVS--YSATSPSLTSLRSPYFFRAT 148

Query: 64  HHITFHMQCTAALVGHFQWHKVTVIYEHTNDMSFNMEALTLLSNQLGVFHAEIEQISSFS 123
           +  +  +    A++  F W +V  +Y    D +F    +  L++ L   +  I   S   
Sbjct: 149 YEDSSQVHAIKAIIKLFGWREVVPVY---IDNTFGEGIMPRLTDSLQDINVRIPYRSVIP 208

Query: 124 SSYTEAMIEEKLKNLVGHERNKVFIVVQFSIELAKLLFHKANKMKMMENGFVWIVGDEIS 183
            + T+  I  +L  ++ +   +VFI V  S  LA  +F KA ++ +M+ G+VWI+ + + 
Sbjct: 209 LNATDQDISVELLKMM-NMPTRVFI-VHMSSSLASTVFIKAKELGLMKPGYVWILTNGVM 268

Query: 184 SHLDSLDSSTFNDMQGVIGFRTYFDHNKDSFKKFRTKFLRKYVLEYHHQEEEMKNGEPSI 243
             L S++ +    M+GV+G +TY   +KD  + FR+++ R++              E ++
Sbjct: 269 DGLRSINETGIEAMEGVLGIKTYIPKSKD-LETFRSRWKRRF-----------PQMELNV 328

Query: 244 FALRAYDASWAVALAMNKLQGN---FTN-------------------KQLLKEILGSEFE 303
           + L AYDA+ A+A+A+     N   F+N                    +LL+ +   +F+
Sbjct: 329 YGLWAYDATTALAMAIEDAGINNMTFSNVDTGKNVSELDGLGLSQFGPKLLQTVSTVQFK 388

Query: 304 GLSGKIGFKNGALKEPPTFEIIYVVGKSYKEMGFWRQKVGFFSMIENDEEISSIIIDERR 363
           GL+G   F +G L +P  FEI+ ++G   + +GFW +  G    ++           E R
Sbjct: 389 GLAGDFHFVSGQL-QPSVFEIVNMIGTGERSIGFWTEGNGLVKKLD----------QEPR 448

Query: 364 SRSNNNGVLELPRFIFLEGNAKTGLIKGRINVDNSDSRVIGRTLRIGVPANNTFREFVKV 423
           S    +   +  + I   G A   + KG     N      G+ LRIGVP    F + VKV
Sbjct: 449 SIGTLSTWPDHLKHIIWPGEA-VSVPKGWEIPTN------GKKLRIGVPKRIGFTDLVKV 508

Query: 424 SYNHI-NGMYISGFSITVFEAVAKNLPYPLSYQLVPFN-------GSYDGLVKQVYEKDL 483
           + + I N   + GF I  FEAV + +PY +SY+  PF        G+++ LV QVY    
Sbjct: 509 TRDPITNSTVVKGFCIDFFEAVIQAMPYDVSYEFFPFEKPNGEPAGNHNDLVHQVYLGQF 568

Query: 484 DAAVGDIGIFADRFQYVDFTEPYLVSGLLLIVKEETENWKEIWVFMKTFTTTMWL-ILPF 543
           DA VGD  I A+R  +VDFT P++ SG+ LIV  + E  ++ + F+K  +  +WL  L F
Sbjct: 569 DAVVGDTTILANRSSFVDFTLPFMKSGVGLIVPLKDEVKRDKFSFLKPLSIELWLTTLVF 628

Query: 544 SHIFIISIVWLVKEESEDLSG-----FGEMLWFSVTVIFYTQRKEVKGFLARLVLGTWLF 603
             +  IS+  L    + D  G        + WF+ + + +  R+ V  F AR ++ TW F
Sbjct: 629 FFLVGISVWTLEHRVNSDFRGPANYQASTIFWFAFSTMVFAPRERVLSFGARSLVVTWYF 688

Query: 604 VILVVTSSFTASLTSMMTVSRFAPSVVDIETIRQTNATVGCNFHSFIIRYLNDVLHIPSA 663
           V+LV+T S+TASL S++T  +  P++  + ++     TVG    SFI+  LN+     S+
Sbjct: 689 VLLVLTQSYTASLASLLTSQQLNPTITSMSSLLHRGETVGYQRTSFILGKLNETGFPQSS 748

Query: 664 NI--KTLVGIDD-YPKAFDNGEIQAAFFITPHAK------------------------AF 688
            +   T    D+   K   NG + AAF  TP+ +                         F
Sbjct: 749 LVPFDTAEECDELLKKGPKNGGVAAAFLGTPYVRLFLGQYCNTYKMVEEPFNVDGFGFVF 797

BLAST of Lsi08G014760 vs. ExPASy Swiss-Prot
Match: Q7XJL2 (Glutamate receptor 3.1 OS=Arabidopsis thaliana OX=3702 GN=GLR3.1 PE=2 SV=3)

HSP 1 Score: 246.1 bits (627), Expect = 1.4e-63
Identity = 222/786 (28.24%), Postives = 363/786 (46.18%), Query Frame = 0

Query: 11  EVSTILGAFTLQEMQLMSEINKTFIDIPIISLPVAASIPPNGLFPPPSFIQMAHHITFHM 70
           +V  I+G  T     ++S +    + +P++S    A  P       P F+Q A    F M
Sbjct: 98  DVVAIIGPQTSIMAHVLSHLANE-LTVPMLSF--TALDPTLSPLQFPFFVQTAPSDLFLM 157

Query: 71  QCTAALVGHFQWHKVTVIYEHTNDMSFNMEALTLLSNQL-----GVFHAEIEQISSFSSS 130
           +  A ++ ++ W  V  +Y   ND   +   +T L ++L      + +  +  +    +S
Sbjct: 158 RAIAEMITYYGWSDVVALY---NDDDNSRNGVTALGDELEERRCKISYKAVLPLDVVITS 217

Query: 131 YTEAMIEEKLKNLVGHERNKVFIVVQFSIELAKLLFHKANKMKMMENGFVWIVGDEISSH 190
             E +IEE +K  +    ++V +V  F     K++F +A ++ MME G+VWI    +SS 
Sbjct: 218 PVE-IIEELIK--IRGMESRVIVVNTFP-NTGKMIFKEAERLGMMEKGYVWIATTWLSSV 277

Query: 191 LDS---LDSSTFNDMQGVIGFRTYFDHNKDSFKKFRTKFLRKYVLEYHHQEEEMKNGEPS 250
           LDS   LD+   N   GV+  R    H  DS KK      R +   + ++    K    +
Sbjct: 278 LDSNLPLDTKLVN---GVLTLRL---HTPDSRKK------RDFAARWKNKLSNNKTIGLN 337

Query: 251 IFALRAYDASWAVALA------------------MNKLQGNFTN----------KQLLKE 310
           ++ L AYD  W +A A                  +  L+G   N           QLL  
Sbjct: 338 VYGLYAYDTVWIIARAVKTLLEAGGNLSFSNDAKLGSLKGEALNLSALSRFDQGSQLLDY 397

Query: 311 ILGSEFEGLSGKIGFKNGALKEPPTFEIIYVVGKSYKEMGFWRQKVGFFSMIENDEEISS 370
           I+ ++  GL+G + F        P+++II +V     ++G+W    G  S++  +   S 
Sbjct: 398 IVHTKMSGLTGPVQFHPDRSMLQPSYDIINLVDDRVHQIGYWSNYSG-LSIVPPESFYSK 457

Query: 371 IIIDERRSRSNNNGVLELPRFIFLEGNAKTGLIKGRINVDNSDSRVIGRTLRIGVPANNT 430
                 RS SN +    L    +  G + T   +G I  +N      GR LRIGVP   +
Sbjct: 458 ---PPNRSSSNQH----LNSVTWPGGTSVTP--RGWIFRNN------GRRLRIGVPDRAS 517

Query: 431 FREFVKVSYNHINGM--YISGFSITVFEAVAKNLPYPLSYQLVPF-----NGSYDGLVKQ 490
           F++FV    + +NG    + G+ I VFEA  K L YP+ ++ + F     N +Y+ LV +
Sbjct: 518 FKDFV----SRVNGSSNKVQGYCIDVFEAAVKLLSYPVPHEFIFFGDGLTNPNYNELVNK 577

Query: 491 VYE-KDLDAAVGDIGIFADRFQYVDFTEPYLVSGLLLIVKEETENWKEIWVFMKTFTTTM 550
           V    D DA VGDI I   R + VDFT+PY+ SGL+++      N +  W F++ FT  M
Sbjct: 578 VTTGVDFDAVVGDIAIVTKRTRIVDFTQPYIESGLVVVAPVTRLN-ENPWAFLRPFTLPM 637

Query: 551 WLILPFSHIFIISIVWLVKEESED------LSGFGEMLWFSVTVIFYTQRKEVKGFLARL 610
           W +     + + + +W+++    D            +LWF+ + +F++ R+     L R+
Sbjct: 638 WAVTASFFVIVGAAIWILEHRINDEFRGPPRRQIITILWFTFSTMFFSHRETTVSTLGRM 697

Query: 611 VLGTWLFVILVVTSSFTASLTSMMTVSRFAPSVVDIETIRQTNATVGCNFHSFIIRYLND 670
           VL  WLFV+L++TSS+TASLTS++TV +    +  ++T+  +   +G    SF   Y+ D
Sbjct: 698 VLLIWLFVVLIITSSYTASLTSILTVQQLNSPIKGVDTLISSTGRIGFQVGSFAENYMTD 757

Query: 671 VLHIPSANIKTLVGIDDYPKAFDNGEIQAA--------FFITPHAK-------------- 722
            L+I S+ +  L   ++Y  A  NG + A          F++ + K              
Sbjct: 758 ELNIASSRLVPLASPEEYANALQNGTVAAIVDERPYIDLFLSDYCKFAIRGQEFTRCGWG 817

BLAST of Lsi08G014760 vs. ExPASy Swiss-Prot
Match: Q9SHV2 (Glutamate receptor 2.3 OS=Arabidopsis thaliana OX=3702 GN=GLR2.3 PE=2 SV=1)

HSP 1 Score: 245.4 bits (625), Expect = 2.3e-63
Identity = 210/752 (27.93%), Postives = 357/752 (47.47%), Query Frame = 0

Query: 1   MSPLDLISIKEVSTILGAFTLQEMQLMSEINKTFIDIPIISLPVAASIPPNGLFPPPSFI 60
           ++ LDLI  K+V  ILG +T  +   + EI +    +PI+S   +A+ P       P F+
Sbjct: 85  IAALDLIKNKQVKAILGPWTSMQAHFLIEIGQK-SRVPIVS--YSATSPILTSLRSPYFL 144

Query: 61  QMAHHITFHMQCTAALVGHFQWHKVTVIYEHTNDMSFNMEALTLLSNQLGVFHAEIEQIS 120
           +  +  +F +Q   A++  F W +V  +Y    D +F    +  L++ L   +  I   S
Sbjct: 145 RATYEDSFQVQPIKAIIKLFGWREVVPVY---IDNTFGEGIMPRLTDALQDINVRIPYRS 204

Query: 121 SFSSSYTEAMIEEKLKNLVGHERNKVFIVVQFSIELAKLLFHKANKMKMMENGFVWIVGD 180
             + + T+  I  +L  ++ +   +VF+V  +  +LA   F KA ++ +ME G+VWI+ +
Sbjct: 205 VIAINATDHEISVELLKMM-NMPTRVFLVHMY-YDLASRFFIKAKELGLMEPGYVWILTN 264

Query: 181 EISSHLDSLDSSTFNDMQGVIGFRTYFDHNKDSFKKFRTKFLRKYVLEYHHQEEEMKNGE 240
            +   L  ++ +    M+GV+G +TY   + D  +KFR+++   +              E
Sbjct: 265 GVIDDLSLINETAVEAMEGVLGIKTYIPKSPD-LEKFRSRWRSLF-----------PRVE 324

Query: 241 PSIFALRAYDASWAVALAMNKL-QGNFT----------------------NKQLLKEILG 300
            S++ L AYDA+ A+A+A+ +    N T                        +LL+ +L 
Sbjct: 325 LSVYGLWAYDATTALAVAIEEAGTNNMTFSKVVDTGRNVSELEALGLSQFGPKLLQTLLT 384

Query: 301 SEFEGLSGKIGFKNGALKEPPTFEIIYVVGKSYKEMGFWRQKVGFFSMIENDEEISSIII 360
            +F GL+G+  F  G L +P  FEI+ ++    K +GFW++  G    +  D++ SSI  
Sbjct: 385 VQFRGLAGEFRFFRGQL-QPSVFEIVNIINTGEKSIGFWKEGNGLVKKL--DQQASSI-- 444

Query: 361 DERRSRSNNNGVLELPRFIFLEGNAKTGLIKGRINVDNSDSRVIGRTLRIGVPANNTFRE 420
                 S  +   +  + I   G A +     +I          G+ LRIGVP    + +
Sbjct: 445 ------SALSTWKDHLKHIVWPGEADSVPKGWQIPTK-------GKKLRIGVPKRTGYTD 504

Query: 421 FVKVSYNHI-NGMYISGFSITVFEAVAKNLPYPLSYQLVPFN-------GSYDGLVKQVY 480
            VKV+ + I N   ++GF I  FEAV + LPY +SY+ +PF        G+Y+ LV QVY
Sbjct: 505 LVKVTRDPITNSTVVTGFCIDFFEAVIRELPYDVSYEFIPFEKPDGKTAGNYNDLVYQVY 564

Query: 481 EKDLDAAVGDIGIFADRFQYVDFTEPYLVSGLLLIVKEETENWKEIWVFMKTFTTTMWLI 540
               DA VGD  I  +R  YVDFT P++ SG+ LIV+      ++  +FMK  +  +WL 
Sbjct: 565 LGRYDAVVGDTTILVNRSSYVDFTFPFIKSGVGLIVEMTDPVKRDYILFMKPLSWKLWLT 624

Query: 541 LPFSHIFIISIVWLVK-EESEDLSG-----FGEMLWFSVTVIFYTQRKEVKGFLARLVLG 600
              S   +   VW+++ + + D SG        + WF+ + + +  R+ V  F AR ++ 
Sbjct: 625 SFISFFLVGCTVWVLEYKRNPDFSGPPRFQASTICWFAFSTMVFAPRERVFSFWARALVI 684

Query: 601 TWLFVILVVTSSFTASLTSMMTVSRFAPSVVDIETIRQTNATVGCNFHSFIIRYLNDVLH 660
            W F++LV+T S+TASL S++T  +  P++  + ++ +   TVG    SFI+  L +   
Sbjct: 685 AWYFLVLVLTQSYTASLASLLTSQKLNPTITSMSSLLEKGETVGYQRTSFILGKLKE-RG 744

Query: 661 IPSANI---KTLVGIDD-YPKAFDNGEIQAAFFITPHAK--------------------- 688
            P +++    T    D+   K    G +  AF   P+ +                     
Sbjct: 745 FPQSSLVPFDTAEECDELLSKGPKKGGVSGAFLEIPYLRLFLGQFCNTYKMVEEPFNVDG 797

BLAST of Lsi08G014760 vs. ExPASy TrEMBL
Match: A0A5A7V316 (Glutamate receptor 2.5-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00390 PE=3 SV=1)

HSP 1 Score: 1240.3 bits (3208), Expect = 0.0e+00
Identity = 654/851 (76.85%), Postives = 716/851 (84.14%), Query Frame = 0

Query: 1    MSPLDLISIKEVSTILGAFTLQEMQLMSEINKTFIDIPIISLPVAASIPP--NGLFPPPS 60
            +S LDLI  KEVSTILGAFT QEMQLMSEIN  FIDIPIISLP+AAS+ P  N LFP PS
Sbjct: 1211 ISALDLIGNKEVSTILGAFTSQEMQLMSEINTNFIDIPIISLPIAASLTPHNNNLFPHPS 1270

Query: 61   FIQMAHHITFHMQCTAALVGHFQWHKVTVIYEHTNDMSFNMEALTLLSNQLGVFHAEIEQ 120
            FIQMA +ITFH+QCTAA+V HFQWHKVT+IY+ TNDMSFNMEALTLLSNQLG F+ EI+Q
Sbjct: 1271 FIQMAQNITFHIQCTAAVVAHFQWHKVTLIYDITNDMSFNMEALTLLSNQLGAFNVEIDQ 1330

Query: 121  ISSFSSSYTEAMIEEKLKNLVGHERNKVFIVVQFSIELAKLLFHKANKMKMMENGFVWIV 180
            ISSFSSSYTE+MIEEKLK+LVG ERNKVFI+VQFSIELAKLLFHKA +M MM+NGFVWIV
Sbjct: 1331 ISSFSSSYTESMIEEKLKSLVGRERNKVFILVQFSIELAKLLFHKAKEMNMMDNGFVWIV 1390

Query: 181  GDEISSHLDSLDSSTFNDMQGVIGFRTYFDHNKDSFKKFRTKFLRKYVLEYHHQEEEMKN 240
            GDEISSHLDSL SSTFNDMQGVIGFRTYFD NKDSFKKFR+KF RKYV EY  ++EEM N
Sbjct: 1391 GDEISSHLDSLGSSTFNDMQGVIGFRTYFDRNKDSFKKFRSKFQRKYVSEYDDEDEEMTN 1450

Query: 241  GEPSIFALRAYDASWAVALAMNKLQGNFTNKQLLKEILGSEFEGLSGKIGFKNGALKEPP 300
            GEP+IFALRAYDA  AVALA++KLQ NF+NKQLLKEIL  EFEGLSGKIG KNG L EPP
Sbjct: 1451 GEPTIFALRAYDAGCAVALAVHKLQANFSNKQLLKEILRIEFEGLSGKIGVKNGVLMEPP 1510

Query: 301  TFEIIYVVGKSYKEMGFWRQKVGFF-SMIENDEE--ISSIIIDERRSRS--------NNN 360
            TFEIIYVVGKSYK MGFWR+KVGFF +MIEN+++   SSIII   RSRS        NNN
Sbjct: 1511 TFEIIYVVGKSYKGMGFWREKVGFFNNMIENNDQEMSSSIIIHGGRSRSSNNNDDNNNNN 1570

Query: 361  GVLELPRFIFLEGNAKTGLIKGRINVDNSDSRVIGRTLRIGVPANNTFREFVKVSYNHIN 420
             VLELPRF+  EGNA TGLIK RI+V+NS+  V GR L+IGVPANNTF++FV+V Y+H+N
Sbjct: 1571 VVLELPRFVLWEGNAGTGLIKRRIDVENSNFGVTGRILKIGVPANNTFQDFVRVCYSHLN 1630

Query: 421  GMYISGFSITVFEAVAKNLPYPLSYQLVPFNGSYDGLVKQVYEKDLDAAVGDIGIFADRF 480
            GMYISGFSITVFEAVAKNLPYPL YQLVPFNGSYDGL++QVY K LD AVGDIGI ADRF
Sbjct: 1631 GMYISGFSITVFEAVAKNLPYPLLYQLVPFNGSYDGLIEQVYTKGLDGAVGDIGIIADRF 1690

Query: 481  QYVDFTEPYLVSGLLLIVKEETENWKEIWVFMKTFTTTMWLILPFSHIFIISIVWLVKEE 540
            +YVDFTEPYLVSGLL+IVKEET+ WKEIW FM+TFTTTMW+ILP SHIFIIS+VWLVKE+
Sbjct: 1691 RYVDFTEPYLVSGLLMIVKEETKIWKEIWAFMRTFTTTMWIILPISHIFIISVVWLVKED 1750

Query: 541  -SEDLSGFGEMLWFSVTVIFYTQRKEVKGFLARLVLGTWLFVILVVTSSFTASLTSMMTV 600
             S+DLSGFGEMLWFS+TVIFY Q++EVKGFLARLVLGTWLFVILVVTSSFTASLTSMMTV
Sbjct: 1751 SSDDLSGFGEMLWFSITVIFYAQKREVKGFLARLVLGTWLFVILVVTSSFTASLTSMMTV 1810

Query: 601  SRFAPSVVDIETIRQTNATVGCNFHSFIIRYLNDVLHIPSANIKTLVGIDDYPKAFDNGE 660
            SRFAPSVVDIET+RQ NATVGCNF+SFIIRYLNDVL IP ANIKTL G+D+YPKAFDNG+
Sbjct: 1811 SRFAPSVVDIETLRQMNATVGCNFNSFIIRYLNDVLKIPLANIKTLSGLDEYPKAFDNGQ 1870

Query: 661  IQAAFFITPHAK-----------------------AFPKGSTLAVDVSTSIIELIERRKM 720
            I+AAFFITPHAK                       AFPKGS+LAVDVSTSIIELIERRKM
Sbjct: 1871 IEAAFFITPHAKVFLAKYCRGYTTAATFDLGGLGFAFPKGSSLAVDVSTSIIELIERRKM 1930

Query: 721  PQLETTLLSTFNCSLSSQVDGSSSLGPWPFAGFLLLDGSSSKNDGKFRCSSDDPKTVLNI 780
            PQLETTLLSTFNCS SSQVDGSSSLGPWPFAGFLLL+GS SKNDG F+CSSD+PKTVLNI
Sbjct: 1931 PQLETTLLSTFNCSPSSQVDGSSSLGPWPFAGFLLLNGSFSKNDGNFQCSSDEPKTVLNI 1990

Query: 781  GVIADNRSRVGREHIIAIEM------------------------------GLDLISNKEV 785
            GVIAD+ SR GREHIIAI+M                               LDLISNKEV
Sbjct: 1991 GVIADSSSRAGREHIIAIQMAVEDYIFTSCHKVELLLVDSPENSAQTTATSLDLISNKEV 2050

BLAST of Lsi08G014760 vs. ExPASy TrEMBL
Match: A0A1S3BAS6 (glutamate receptor 2.1-like OS=Cucumis melo OX=3656 GN=LOC103487887 PE=3 SV=1)

HSP 1 Score: 1155.6 bits (2988), Expect = 0.0e+00
Identity = 601/758 (79.29%), Postives = 658/758 (86.81%), Query Frame = 0

Query: 1   MSPLDLISIKEVSTILGAFTLQEMQLMSEINKTFIDIPIISLPVAASIPP--NGLFPPPS 60
           +S LDLI  KEVSTILGAFT QEMQLMSEIN  FIDIPIISLP+AAS+ P  N LFP PS
Sbjct: 94  ISALDLIGNKEVSTILGAFTSQEMQLMSEINTNFIDIPIISLPIAASLTPHNNNLFPHPS 153

Query: 61  FIQMAHHITFHMQCTAALVGHFQWHKVTVIYEHTNDMSFNMEALTLLSNQLGVFHAEIEQ 120
           FIQMA +ITFH+QCTAA+V HFQWHKVT+IY+ TNDMSFNMEALTLLSNQLG F+ EI+Q
Sbjct: 154 FIQMAQNITFHIQCTAAVVAHFQWHKVTLIYDITNDMSFNMEALTLLSNQLGAFNVEIDQ 213

Query: 121 ISSFSSSYTEAMIEEKLKNLVGHERNKVFIVVQFSIELAKLLFHKANKMKMMENGFVWIV 180
           ISSFSSSYTE+MIEEKLK+LVG ERNKVFI+VQFSIELAKLLFHKA +M MM+NGFVWIV
Sbjct: 214 ISSFSSSYTESMIEEKLKSLVGRERNKVFILVQFSIELAKLLFHKAKEMNMMDNGFVWIV 273

Query: 181 GDEISSHLDSLDSSTFNDMQGVIGFRTYFDHNKDSFKKFRTKFLRKYVLEYHHQEEEMKN 240
           GDEISSHLDSL SSTFNDMQGVIGFRTYFD NKDSFKKFR+KF RKYV EY  ++EEM N
Sbjct: 274 GDEISSHLDSLGSSTFNDMQGVIGFRTYFDRNKDSFKKFRSKFQRKYVSEYDDEDEEMTN 333

Query: 241 GEPSIFALRAYDASWAVALAMNKLQGNFTNKQLLKEILGSEFEGLSGKIGFKNGALKEPP 300
           GEP+IFALRAYDA WAVALA++KLQ NF+NKQLLKEIL  EFEGLSGKIG KNG L EPP
Sbjct: 334 GEPTIFALRAYDAGWAVALAVHKLQANFSNKQLLKEILRIEFEGLSGKIGVKNGVLMEPP 393

Query: 301 TFEIIYVVGKSYKEMGFWRQKVGFF-SMIENDEE--ISSIIIDERRSRS-------NNNG 360
           TFEIIYVVGKSYK MGFWR+KVGFF +MIEN+++   SSIII   RSRS       NNN 
Sbjct: 394 TFEIIYVVGKSYKGMGFWREKVGFFNNMIENNDQEMSSSIIIHGGRSRSSNNNDDNNNNV 453

Query: 361 VLELPRFIFLEGNAKTGLIKGRINVDNSDSRVIGRTLRIGVPANNTFREFVKVSYNHING 420
           VLELPRF+  EGNA TGLIK RI+V+NS+  V GR L+IGVPANNTF++FV+V Y+H+NG
Sbjct: 454 VLELPRFVLWEGNAGTGLIKRRIDVENSNFGVTGRILKIGVPANNTFQDFVRVCYSHLNG 513

Query: 421 MYISGFSITVFEAVAKNLPYPLSYQLVPFNGSYDGLVKQVYEKDLDAAVGDIGIFADRFQ 480
           MYISGFSITVFEAVAKNLPYPL YQLVPFNGSYDGL++QVY K LD AVGDIGI ADRF+
Sbjct: 514 MYISGFSITVFEAVAKNLPYPLLYQLVPFNGSYDGLIEQVYTKGLDGAVGDIGIIADRFR 573

Query: 481 YVDFTEPYLVSGLLLIVKEETENWKEIWVFMKTFTTTMWLILPFSHIFIISIVWLVKEE- 540
           YVDFTEPYLVSGLL+IVKEET+ WKEIW FM+TFTTTMW+ILP SHIFIIS+VWLVKE+ 
Sbjct: 574 YVDFTEPYLVSGLLMIVKEETKIWKEIWAFMRTFTTTMWIILPISHIFIISVVWLVKEDS 633

Query: 541 SEDLSGFGEMLWFSVTVIFYTQRKEVKGFLARLVLGTWLFVILVVTSSFTASLTSMMTVS 600
           S+DLSGFGEMLWFS+TVIFY Q++EVKGFLARLVLGTWLFVILVVTSSFTASLTSMMTVS
Sbjct: 634 SDDLSGFGEMLWFSITVIFYAQKREVKGFLARLVLGTWLFVILVVTSSFTASLTSMMTVS 693

Query: 601 RFAPSVVDIETIRQTNATVGCNFHSFIIRYLNDVLHIPSANIKTLVGIDDYPKAFDNGEI 660
           RFAPSVVDIET+RQ NATVGCNF+SFIIRYLNDVL IP ANIKTL G+D+YPKAFDNG+I
Sbjct: 694 RFAPSVVDIETLRQMNATVGCNFNSFIIRYLNDVLKIPLANIKTLSGLDEYPKAFDNGQI 753

Query: 661 QAAFFITPHAK-----------------------AFPKGSTLAVDVSTSIIELIERRKMP 720
           +AAFFITPHAK                       AFPKGS+LAVDVSTSIIELIERRKMP
Sbjct: 754 EAAFFITPHAKVFLAKYCRGYTTAATFDLGGLGFAFPKGSSLAVDVSTSIIELIERRKMP 813

Query: 721 QLETTLLSTFNCSLSSQVDGSSSLGPWPFAGFLLLDGS 723
           QLETTLLSTFNCS SSQVDGSSSLGPWPFAG   L GS
Sbjct: 814 QLETTLLSTFNCSPSSQVDGSSSLGPWPFAGLFFLSGS 851

BLAST of Lsi08G014760 vs. ExPASy TrEMBL
Match: A0A1S3BBI6 (glutamate receptor 2.1-like OS=Cucumis melo OX=3656 GN=LOC103487897 PE=3 SV=1)

HSP 1 Score: 1114.0 bits (2880), Expect = 0.0e+00
Identity = 582/758 (76.78%), Postives = 651/758 (85.88%), Query Frame = 0

Query: 2   SPLDLISIKEVSTILGAFTLQEMQLMSEINKTFIDIPIISLPVAASIPP--NGLFPPPSF 61
           S LDLI  KEVSTILGAFTLQEMQLMSEINK FIDI IISLP+AAS+PP  N LFP PSF
Sbjct: 89  SALDLIGNKEVSTILGAFTLQEMQLMSEINKNFIDISIISLPIAASLPPHKNNLFPLPSF 148

Query: 62  IQMAHHITFHMQCTAALVGHFQWHKVTVIYEHTNDMSFNMEALTLLSNQLGVFHAEIEQI 121
           I+MAH+ITFH+QCTAA+V HFQWHKVT+IY++TND+ FNMEALTLLSNQLG F  EI+QI
Sbjct: 149 IRMAHNITFHIQCTAAIVAHFQWHKVTLIYDNTNDVFFNMEALTLLSNQLGAFDVEIDQI 208

Query: 122 SSFSSSYTEAMIEEKLKNLVGHERNKVFIVVQFSIELAKLLFHKANKMKMMENGFVWIVG 181
           S FSSSY+E+MIEEKLK+LVG ER+KVFI+VQFS+ELAK LFHKANKM MM+NGFVWIVG
Sbjct: 209 SYFSSSYSESMIEEKLKSLVGRERSKVFILVQFSVELAKFLFHKANKMNMMDNGFVWIVG 268

Query: 182 DEISSHLDSLDSSTFNDMQGVIGFRTYFDHNKDSFKKFRTKFLRKYVLEYH-HQEEEMKN 241
           DEISSHLDSLDSSTFNDMQGVIGFRTYFDHNKD+FKKFR+KF RKYVLEY+  +EEEMKN
Sbjct: 269 DEISSHLDSLDSSTFNDMQGVIGFRTYFDHNKDTFKKFRSKFHRKYVLEYNDDEEEEMKN 328

Query: 242 GEPSIFALRAYDASWAVALAMNKLQGNFTNKQLLKEILGSEFEGLSGKIGFKNGALKEPP 301
           GEP+IFALRAYDA WAVALAM+KLQ NF+NKQL KEIL SEFEGLSGKIGFKNG L EPP
Sbjct: 329 GEPTIFALRAYDAGWAVALAMHKLQANFSNKQLSKEILRSEFEGLSGKIGFKNGVLMEPP 388

Query: 302 TFEIIYVVGKSYKEMGFWRQKVGFFSMI--ENDEEI-SSIIIDERRSRS---NNNGVLEL 361
           TFEIIYVVGKSYKEMGFWR+KVGFF+ +   ND+EI SSIIIDE RSRS   NN+ VL+L
Sbjct: 389 TFEIIYVVGKSYKEMGFWREKVGFFNNLNENNDQEISSSIIIDEGRSRSDNKNNDVVLKL 448

Query: 362 PRFIFLEGN-AKTGLIKGR-INVDNSDSRVIGRT-LRIGVPANNTFREFVKVSYNHINGM 421
           PRF+  E N A+T L+K R IN+DNS+S  +GRT LRIG+PANNTFREFVKVSY+HING 
Sbjct: 449 PRFVLWEVNYAETELMKRRTINIDNSNSGGMGRTLLRIGIPANNTFREFVKVSYDHINGK 508

Query: 422 YISGFSITVFEAVAKNLPYPLSYQLVPFNGSYDGLVKQVYEKDLDAAVGDIGIFADRFQY 481
           YISGFSI+VFEAV KNLPY LSYQL+P NGSYDGL+KQVY K LDAAVGDIGI+ADRFQY
Sbjct: 509 YISGFSISVFEAVVKNLPYSLSYQLIPINGSYDGLIKQVYAKGLDAAVGDIGIYADRFQY 568

Query: 482 VDFTEPYLVSGLLLIVKEETENWKEIWVFMKTFTTTMWLILPFSHIFIISIVWLVKEESE 541
           VDFTEPY++ GL++IVKEET NWK+IW+FMKTFTT MW+ILP  H+ I+S+VW V++ ++
Sbjct: 569 VDFTEPYMMGGLVMIVKEETRNWKQIWIFMKTFTTPMWIILPIFHLVIMSVVWFVRDRND 628

Query: 542 -DL-SGFGEMLWFSVTVIFYTQRKEVKGFLARLVLGTWLFVILVVTSSFTASLTSMMTVS 601
            DL  G  EMLWF+VTVIFY QRKEVKG LARLVLGTWLFVILVVTSSFTASLTSMMTVS
Sbjct: 629 RDLPPGIPEMLWFAVTVIFYAQRKEVKGNLARLVLGTWLFVILVVTSSFTASLTSMMTVS 688

Query: 602 RFAPSVVDIETIRQTNATVGCNFHSFIIRYLNDVLHIPSANIKTLVGIDDYPKAFDNGEI 661
           RFAPSVVDIET+RQ NATVGCN+HSFI RYLN  L IPS NIK  VGIDDYPK+FDNGEI
Sbjct: 689 RFAPSVVDIETLRQMNATVGCNYHSFIPRYLNHTLKIPSENIKNFVGIDDYPKSFDNGEI 748

Query: 662 QAAFFITPHAK-----------------------AFPKGSTLAVDVSTSIIELIERRKMP 721
           +AAFFITPH+K                       AF KGS+LAVDVS SI+ELIE+R+MP
Sbjct: 749 EAAFFITPHSKVFLARYCKGYTIAATFNLGGIGFAFRKGSSLAVDVSKSIVELIEKREMP 808

Query: 722 QLETTLLSTFNCSLSSQVDGSSSLGPWPFAGFLLLDGS 723
           QLETTLLSTFNCS  SQVDGSSSLGPWPFAG  ++  S
Sbjct: 809 QLETTLLSTFNCSSGSQVDGSSSLGPWPFAGLFIISAS 846

BLAST of Lsi08G014760 vs. ExPASy TrEMBL
Match: A0A5A7V019 (Glutamate receptor 2.1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00210 PE=3 SV=1)

HSP 1 Score: 1108.6 bits (2866), Expect = 0.0e+00
Identity = 580/750 (77.33%), Postives = 647/750 (86.27%), Query Frame = 0

Query: 2   SPLDLISIKEVSTILGAFTLQEMQLMSEINKTFIDIPIISLPVAASIPP--NGLFPPPSF 61
           S LDLI  KEVSTILGAFTLQEMQLMSEINK FIDI IISLP+AAS+PP  N LFP PSF
Sbjct: 89  SALDLIGNKEVSTILGAFTLQEMQLMSEINKNFIDISIISLPIAASLPPHKNNLFPLPSF 148

Query: 62  IQMAHHITFHMQCTAALVGHFQWHKVTVIYEHTNDMSFNMEALTLLSNQLGVFHAEIEQI 121
           I+MAH+ITFH+QCTAA+V HFQWHKVT+IY++TND+ FNMEALTLLSNQLG F  EI+QI
Sbjct: 149 IRMAHNITFHIQCTAAIVAHFQWHKVTLIYDNTNDVFFNMEALTLLSNQLGAFDVEIDQI 208

Query: 122 SSFSSSYTEAMIEEKLKNLVGHERNKVFIVVQFSIELAKLLFHKANKMKMMENGFVWIVG 181
           S FSSSY+E+MIEEKLK+LVG ER+KVFI+VQFS+ELAK LFHKANKM MM+NGFVWIVG
Sbjct: 209 SYFSSSYSESMIEEKLKSLVGRERSKVFILVQFSVELAKFLFHKANKMNMMDNGFVWIVG 268

Query: 182 DEISSHLDSLDSSTFNDMQGVIGFRTYFDHNKDSFKKFRTKFLRKYVLEYH-HQEEEMKN 241
           DEISSHLDSLDSSTFNDMQGVIGFRTYFDHNKD+FKKFR+KF RKYVLEY+  +EEEMKN
Sbjct: 269 DEISSHLDSLDSSTFNDMQGVIGFRTYFDHNKDTFKKFRSKFHRKYVLEYNDDEEEEMKN 328

Query: 242 GEPSIFALRAYDASWAVALAMNKLQGNFTNKQLLKEILGSEFEGLSGKIGFKNGALKEPP 301
           GEP+IFALRAYDA WAVALAM+KLQ NF+NKQL KEIL SEFEGLSGKIGFKNG L EPP
Sbjct: 329 GEPTIFALRAYDAGWAVALAMHKLQANFSNKQLSKEILRSEFEGLSGKIGFKNGVLMEPP 388

Query: 302 TFEIIYVVGKSYKEMGFWRQKVGFFSMI--ENDEEI-SSIIIDERRSRS---NNNGVLEL 361
           TFEIIYVVGKSYKEMGFWR+KVGFF+ +   ND+EI SSIIIDE RSRS   NN+ VL+L
Sbjct: 389 TFEIIYVVGKSYKEMGFWREKVGFFNNLNENNDQEISSSIIIDEGRSRSDNKNNDVVLKL 448

Query: 362 PRFIFLEGN-AKTGLIKGR-INVDNSDSRVIGRT-LRIGVPANNTFREFVKVSYNHINGM 421
           PRF+  E N A+T L+K R IN+DNS+S  +GRT LRIG+PANNTFREFVKVSY+HING 
Sbjct: 449 PRFVLWEVNYAETELMKRRTINIDNSNSGGMGRTLLRIGIPANNTFREFVKVSYDHINGK 508

Query: 422 YISGFSITVFEAVAKNLPYPLSYQLVPFNGSYDGLVKQVYEKDLDAAVGDIGIFADRFQY 481
           YISGFSI+VFEAV KNLPY LSYQL+P NGSYDGL+KQVY K LDAAVGDIGI+ADRFQY
Sbjct: 509 YISGFSISVFEAVVKNLPYSLSYQLIPINGSYDGLIKQVYAKGLDAAVGDIGIYADRFQY 568

Query: 482 VDFTEPYLVSGLLLIVKEETENWKEIWVFMKTFTTTMWLILPFSHIFIISIVWLVKEESE 541
           VDFTEPY++ GL++IVKEET NWK+IW+FMKTFTT MW+ILP  H+ I+S+VW V++ ++
Sbjct: 569 VDFTEPYMMGGLVMIVKEETRNWKQIWIFMKTFTTPMWIILPIFHLVIMSVVWFVRDRND 628

Query: 542 -DL-SGFGEMLWFSVTVIFYTQRKEVKGFLARLVLGTWLFVILVVTSSFTASLTSMMTVS 601
            DL  G  EMLWF+VTVIFY QRKEVKG LARLVLGTWLFVILVVTSSFTASLTSMMTVS
Sbjct: 629 RDLPPGIPEMLWFAVTVIFYAQRKEVKGNLARLVLGTWLFVILVVTSSFTASLTSMMTVS 688

Query: 602 RFAPSVVDIETIRQTNATVGCNFHSFIIRYLNDVLHIPSANIKTLVGIDDYPKAFDNGEI 661
           RFAPSVVDIET+RQ NATVGCN+HSFI RYLN  L IPS NIK  VGIDDYPK+FDNGEI
Sbjct: 689 RFAPSVVDIETLRQMNATVGCNYHSFIPRYLNHTLKIPSENIKNFVGIDDYPKSFDNGEI 748

Query: 662 QAAFFITPHAK-----------------------AFPKGSTLAVDVSTSIIELIERRKMP 715
           +AAFFITPH+K                       AF KGS+LAVDVS SI+ELIE+R+MP
Sbjct: 749 EAAFFITPHSKVFLARYCKGYTIAATFNLGGIGFAFRKGSSLAVDVSKSIVELIEKREMP 808

BLAST of Lsi08G014760 vs. ExPASy TrEMBL
Match: A0A6J1K765 (glutamate receptor 2.5-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492269 PE=3 SV=1)

HSP 1 Score: 1086.2 bits (2808), Expect = 0.0e+00
Identity = 576/794 (72.54%), Postives = 642/794 (80.86%), Query Frame = 0

Query: 2   SPLDLISIKEVSTILGAFTLQEMQLMSEINKTFIDIPIISLPVAASIPPNGLFPPPSFIQ 61
           S LDLIS KEVS ILGAFT QEMQ +SEINKT +DI  ISLPVAAS+PP  L P PSFIQ
Sbjct: 84  SALDLISSKEVSAILGAFTFQEMQFISEINKTSVDIATISLPVAASVPP--LLPLPSFIQ 143

Query: 62  MAHHITFHMQCTAALVGHFQWHKVTVIYEHTNDMSFNMEALTLLSNQLGVFHAEIEQISS 121
           MAHHITFHMQC AA+VGHFQWHKVTVIYE+ NDMS NMEA TLLSN+L VF+AEIEQIS+
Sbjct: 144 MAHHITFHMQCAAAIVGHFQWHKVTVIYENRNDMSINMEAFTLLSNELRVFNAEIEQISA 203

Query: 122 FSSSYTEAMIEEKLKNLVGHERNKVFIVVQFSIELAKLLFHKANKMKMMENGFVWIVGDE 181
           FSSS+TEAMIEEKLK+L+G +RN+VFIVVQFSIELAKLLFH+A +M MM+NGFVWIVGDE
Sbjct: 204 FSSSHTEAMIEEKLKSLMGRDRNRVFIVVQFSIELAKLLFHRAKRMNMMDNGFVWIVGDE 263

Query: 182 ISSHLDSLDSSTFNDMQGVIGFRTYFDHNKDSFKKFRTKFLRKYVLEYHH----QEEEMK 241
           ISS +DSLDSS F +MQ VIGFRTYF+H+KDSFKKFR KF R Y LEY H    +EEE K
Sbjct: 264 ISSLMDSLDSSNFYNMQDVIGFRTYFNHSKDSFKKFRRKFRRMYRLEYQHHDEEEEEEEK 323

Query: 242 NGEPSIFALRAYDASWAVALAMNKLQGNFTNKQLLKEILGSEFEGLSGKIGFKNGALKEP 301
           + EPSIFALRAYDASWAVA AMNKLQGNFTNKQLLK+IL +EFEGLSG IGF+NG LK+P
Sbjct: 324 SAEPSIFALRAYDASWAVAAAMNKLQGNFTNKQLLKQILATEFEGLSGSIGFENGTLKQP 383

Query: 302 PTFEIIYVVGKSYKEMGFWRQKVGFFSMIENDEEISSIIIDERRSRSNNNGVLELPRFIF 361
           PTFEIIYVVGKSYKEMGFWRQKVGFF+ +  DEEI              NGVLE PRF+F
Sbjct: 384 PTFEIIYVVGKSYKEMGFWRQKVGFFNSLMEDEEI-------------KNGVLEFPRFVF 443

Query: 362 LEGNAKTGLIKGRINVDNSDSRVIGRTLRIGVPANNTFREFVKVSYNHINGMYISGFSIT 421
            EGN +TGL K R+N+D++      R L+IGVPANNTF EFVKVSY+HING+YISG+SI+
Sbjct: 444 WEGNEQTGL-KRRMNLDSNSEE--HRILKIGVPANNTFHEFVKVSYDHINGVYISGYSIS 503

Query: 422 VFEAVAKNLPYPLSYQLVPFNGSYDGLVKQVYEKDLDAAVGDIGIFADRFQYVDFTEPYL 481
           VFEAV KNLPYPL YQLVPFNGSYD LVKQV+ K LDAAVGDIGIFADRFQYVDFTE Y+
Sbjct: 504 VFEAVVKNLPYPLHYQLVPFNGSYDELVKQVHAKGLDAAVGDIGIFADRFQYVDFTESYM 563

Query: 482 VSGLLLIVKEETENWKEIWVFMKTFTTTMWLILPFSHIFIISIVWLVKEESEDL-SGFGE 541
           VSGLL+IVKEE  NWKEIWVFMKTFTTTMW+ILP SH+FIIS+VW V+ ESE L SGFG+
Sbjct: 564 VSGLLMIVKEEKRNWKEIWVFMKTFTTTMWIILPMSHMFIISVVWFVRPESERLKSGFGD 623

Query: 542 MLWFSVTVIFYTQRKEVKGFLARLVLGTWLFVILVVTSSFTASLTSMMTVSRFAPSVVDI 601
           MLWF+++V+F   R+EV G LARLVL  WLFVILVVTSSFTASLTSMMTVSRFAPSVVDI
Sbjct: 624 MLWFAISVVFNAHREEVGGGLARLVLAPWLFVILVVTSSFTASLTSMMTVSRFAPSVVDI 683

Query: 602 ETIRQTNATVGCNFHSFIIRYLNDVLHIPSANIKTLVGIDDYPKAFDNGEIQAAFFITPH 661
           ET+RQTNATVGCNFHSFI+RYLN+VL I   NIKTL  IDDYPKAFDNGEIQAAFFITPH
Sbjct: 684 ETLRQTNATVGCNFHSFIMRYLNNVLQISPDNIKTLSSIDDYPKAFDNGEIQAAFFITPH 743

Query: 662 AK-----------------------AFPKGSTLAVDVSTSIIELIERRKMPQLETTLLST 721
           AK                       AFPKGSTLAVDVSTSIIELIERRKMPQL+T LLST
Sbjct: 744 AKVFLAKYCKGYTTAATFDLGGIGFAFPKGSTLAVDVSTSIIELIERRKMPQLDTMLLST 803

Query: 722 FNCSLSSQVDGSSSLGPWPFAGFLLLDGSSSKNDGKFRCSSDDPKTVLNIGVIADNRSRV 768
           FNCSL SQVDG+S LGPWPFAG  ++ GS +   G   C+      V  +G    N  R 
Sbjct: 804 FNCSLLSQVDGTSRLGPWPFAGLFIVSGSIAL--GVLLCT-----VVKRVG----NWRRR 848

BLAST of Lsi08G014760 vs. NCBI nr
Match: KGN62744.2 (hypothetical protein Csa_018680 [Cucumis sativus])

HSP 1 Score: 1263.1 bits (3267), Expect = 0.0e+00
Identity = 662/851 (77.79%), Postives = 725/851 (85.19%), Query Frame = 0

Query: 1   MSPLDLISIKEVSTILGAFTLQEMQLMSEINKTFIDIPIISLPVAASIPP--NGLFPPPS 60
           +S LDLI  KE+STILGAFTLQE+QLMSEINK FIDI IISLPVAAS+PP  N LFP PS
Sbjct: 92  ISALDLIGNKEMSTILGAFTLQEIQLMSEINKNFIDISIISLPVAASLPPHNNNLFPLPS 151

Query: 61  FIQMAHHITFHMQCTAALVGHFQWHKVTVIYEHTNDMSFNMEALTLLSNQLGVFHAEIEQ 120
           FIQMAH+ITFH+QCTAA+V HF+WHKVT+IY++TND+SFNMEALTLLSNQLG F+ EI+Q
Sbjct: 152 FIQMAHNITFHIQCTAAIVAHFEWHKVTLIYDNTNDISFNMEALTLLSNQLGAFNVEIDQ 211

Query: 121 ISSFSSSYTEAMIEEKLKNLVGHERNKVFIVVQFSIELAKLLFHKANKMKMMENGFVWIV 180
           ISSFSSSYTE+MIEEKLK+LVG ERN+VFI+VQFSIELAKLLFHKA KM MM+NGFVWIV
Sbjct: 212 ISSFSSSYTESMIEEKLKSLVGGERNRVFILVQFSIELAKLLFHKAKKMNMMDNGFVWIV 271

Query: 181 GDEISSHLDSLDSSTFNDMQGVIGFRTYFDHNKDSFKKFRTKFLRKYVLEYHHQEEEMKN 240
           GDEISSHLDS DSSTF+DMQGVIGFRTYFDHNKDSFKKFR+KF RKY  EY  +EEEMKN
Sbjct: 272 GDEISSHLDSSDSSTFSDMQGVIGFRTYFDHNKDSFKKFRSKFQRKYASEYDDEEEEMKN 331

Query: 241 GEPSIFALRAYDASWAVALAMNKLQGNFTNKQLLKEILGSEFEGLSGKIGFKNGALKEPP 300
           GEPSIFALRAYDA WAVALAM+KLQ NF+NKQLLKEIL SEFEGLSGKIGFKNG LKEPP
Sbjct: 332 GEPSIFALRAYDAGWAVALAMHKLQANFSNKQLLKEILRSEFEGLSGKIGFKNGVLKEPP 391

Query: 301 TFEIIYVVGKSYKEMGFWRQKVGFF-SMIENDEE--ISSIIIDERRSRS-------NNNG 360
           TFEIIYVVGKSYKEMGFWR+ VGFF +MIEN+++   SSIII E RSRS       N NG
Sbjct: 392 TFEIIYVVGKSYKEMGFWRENVGFFNNMIENNDQEMSSSIIIHEGRSRSSSNNNDDNKNG 451

Query: 361 VLELPRFIFLEGNAKTGLIKGR-INVDNSDSRVIGRTLRIGVPANNTFREFVKVSYNHIN 420
           VLELPRF+  EGNA TGLIK R I+V+NS+  V GR L+IGVPANNTF++FV+V YNH+N
Sbjct: 452 VLELPRFVLWEGNAGTGLIKRRMIDVENSNFGVTGRILKIGVPANNTFQDFVRVCYNHLN 511

Query: 421 GMYISGFSITVFEAVAKNLPYPLSYQLVPFNGSYDGLVKQVYEKDLDAAVGDIGIFADRF 480
           GMYISGFSITVFEAVAKNLPYPL YQLVPFNGSYDGLV+QVY K LD AVGDIGIFADRF
Sbjct: 512 GMYISGFSITVFEAVAKNLPYPLLYQLVPFNGSYDGLVEQVYTKGLDGAVGDIGIFADRF 571

Query: 481 QYVDFTEPYLVSGLLLIVKEETENWKEIWVFMKTFTTTMWLILPFSHIFIISIVWLVKEE 540
           +YVDFTEPYLVSGLL+IVKE+T+ WKEIW FMKTFTTTMW+ILP SHIFIIS+VWLVK++
Sbjct: 572 RYVDFTEPYLVSGLLMIVKEKTKIWKEIWAFMKTFTTTMWIILPISHIFIISVVWLVKDD 631

Query: 541 S-EDLSGFGEMLWFSVTVIFYTQRKEVKGFLARLVLGTWLFVILVVTSSFTASLTSMMTV 600
           S +D SGFGEMLWFS+TVIFY Q++EVKGFLARLVLGTWLFVILVVTSSFTASLTSMMTV
Sbjct: 632 SGDDPSGFGEMLWFSITVIFYAQKREVKGFLARLVLGTWLFVILVVTSSFTASLTSMMTV 691

Query: 601 SRFAPSVVDIETIRQTNATVGCNFHSFIIRYLNDVLHIPSANIKTLVGIDDYPKAFDNGE 660
           SRFAPSVVDIET+RQ NATVGCNF+SFIIRYLNDVL IP +NIK L G+D+YPKAFDNGE
Sbjct: 692 SRFAPSVVDIETLRQMNATVGCNFNSFIIRYLNDVLKIPLSNIKALSGLDEYPKAFDNGE 751

Query: 661 IQAAFFITPHAK-----------------------AFPKGSTLAVDVSTSIIELIERRKM 720
           I+AAFFITPHAK                       AFPKGS+LAVDVSTSIIELIERRKM
Sbjct: 752 IEAAFFITPHAKVFLAKYCKGYTTAATFDLGGLGFAFPKGSSLAVDVSTSIIELIERRKM 811

Query: 721 PQLETTLLSTFNCSLSSQVDGSSSLGPWPFAGFLLLDGSSSKNDGKFRCSSDDPKTVLNI 780
           PQLETTLLSTFNCS SSQVDGSSSLGPWPFAGFLLL+GSSSK D  F+CSSD+PKTVLN+
Sbjct: 812 PQLETTLLSTFNCSPSSQVDGSSSLGPWPFAGFLLLNGSSSKKDANFQCSSDEPKTVLNV 871

Query: 781 GVIADNRSRVGREHIIAIEM------------------------------GLDLISNKEV 785
           GVIADN SRVGREHIIAI+M                               LDLISNKEV
Sbjct: 872 GVIADNSSRVGREHIIAIQMAVKDYIFTSCYKVELLLLDSPENSAQTTATSLDLISNKEV 931

BLAST of Lsi08G014760 vs. NCBI nr
Match: XP_038885765.1 (glutamate receptor 2.1-like [Benincasa hispida])

HSP 1 Score: 1242.6 bits (3214), Expect = 0.0e+00
Identity = 631/746 (84.58%), Postives = 679/746 (91.02%), Query Frame = 0

Query: 2   SPLDLISIKEVSTILGAFTLQEMQLMSEINKTFIDIPIISLPVAASIPPNGLFPPPSFIQ 61
           S LDLIS  +VSTILGAFTLQEMQ+MSEIN  FIDIPIISLP+AASIPP  LFPPP F Q
Sbjct: 84  SALDLISNNKVSTILGAFTLQEMQIMSEINTNFIDIPIISLPIAASIPPPLLFPPPYFFQ 143

Query: 62  MAHHITFHMQCTAALVGHFQWHKVTVIYEHTNDMSFNMEALTLLSNQLGVFHAEIEQISS 121
           MAH+ITFHMQC AALVGHFQWHKV VIYE+ NDMSFNMEALTLLS +LGVFHAEIEQISS
Sbjct: 144 MAHNITFHMQCIAALVGHFQWHKVVVIYENKNDMSFNMEALTLLSKELGVFHAEIEQISS 203

Query: 122 FSSSYTEAMIEEKLKNLVGHERNKVFIVVQFSIELAKLLFHKANKMKMMENGFVWIVGDE 181
           FSSSYTE MIEEKLK+L+GHERN+VF++VQFSIELAKLLFHKANK+KMMENGFVWIVGDE
Sbjct: 204 FSSSYTEDMIEEKLKHLMGHERNRVFVMVQFSIELAKLLFHKANKLKMMENGFVWIVGDE 263

Query: 182 ISSHLDSLDSSTFNDMQGVIGFRTYFDHNKDSFKKFRTKFLRKYVLEYH-HQEEEMKNGE 241
           ISSHLDSLDSS FNDMQGVIGFRTYFD+NKDSFKKF++KFLRKYVLEYH HQEE++KN E
Sbjct: 264 ISSHLDSLDSSIFNDMQGVIGFRTYFDNNKDSFKKFKSKFLRKYVLEYHDHQEEDIKNWE 323

Query: 242 PSIFALRAYDASWAVALAMNKLQGNFTNKQLLKEILGSEFEGLSGKIGFKNGALKEPPTF 301
           P+IFALRAYDA+WAVALAM+KL+ NFTNKQLLKEILGSEFEG+SGKIGFKNG L EPPTF
Sbjct: 324 PTIFALRAYDAAWAVALAMHKLEANFTNKQLLKEILGSEFEGVSGKIGFKNGGLMEPPTF 383

Query: 302 EIIYVVGKSYKEMGFWRQKVGFFSMIENDEEISSIIIDERRSRS-NNNGVLELPRFIFLE 361
           EIIYVVGKSYKEMGFW +KVGFF  +  +EE+SSIIIDERRSR+ NNNGVLE PR +  E
Sbjct: 384 EIIYVVGKSYKEMGFWSEKVGFFKNLIENEEMSSIIIDERRSRNHNNNGVLEFPRVVLWE 443

Query: 362 GNAKTGLIKGRINVDNSDSRVIGRTLRIGVPANNTFREFVKVSYNHINGMYISGFSITVF 421
           GN +TGLIK RINVDN +S VIGRTLRIGVPANNTFREFV+VSY+HINGMYISGFSITVF
Sbjct: 444 GNTETGLIKRRINVDNYNSGVIGRTLRIGVPANNTFREFVRVSYDHINGMYISGFSITVF 503

Query: 422 EAVAKNLPYPLSYQLVPFNGSYDGLVKQVYEKDLDAAVGDIGIFADRFQYVDFTEPYLVS 481
           EAVAKNLPYPLSYQLVPFNGSYDGLV+QVYEK LD AVGDIGIFADRFQYVDFTEPYLVS
Sbjct: 504 EAVAKNLPYPLSYQLVPFNGSYDGLVEQVYEKGLDGAVGDIGIFADRFQYVDFTEPYLVS 563

Query: 482 GLLLIVKEETENWKEIWVFMKTFTTTMWLILPFSHIFIISIVWLVKEESEDLSGFGEMLW 541
           GLL+IVKEET+NWKEIWVFMKTFTTTMW+ILP SH+FIIS+VWLVKEESE+LSGFGEMLW
Sbjct: 564 GLLMIVKEETKNWKEIWVFMKTFTTTMWIILPVSHLFIISVVWLVKEESEELSGFGEMLW 623

Query: 542 FSVTVIFYTQRKEVKGFLARLVLGTWLFVILVVTSSFTASLTSMMTVSRFAPSVVDIETI 601
           F++TVIFY QRKEVKGFLARLVLGTWLFVILVVTSSFTASLTSMMTVSRFAPSVVDIET+
Sbjct: 624 FAITVIFYAQRKEVKGFLARLVLGTWLFVILVVTSSFTASLTSMMTVSRFAPSVVDIETL 683

Query: 602 RQTNATVGCNFHSFIIRYLNDVLHIPSANIKTLVGIDDYPKAFDNGEIQAAFFITPHAK- 661
           RQTNATVGCN+HSFI+RYLNDVL+I +ANI TLVGIDDYPKAFDNGEIQAAFFITPHAK 
Sbjct: 684 RQTNATVGCNYHSFIMRYLNDVLNISNANIMTLVGIDDYPKAFDNGEIQAAFFITPHAKV 743

Query: 662 ----------------------AFPKGSTLAVDVSTSIIELIERRKMPQLETTLLSTFNC 721
                                 AFPKGSTLAVDVSTSIIELIERRKMPQLETTLLSTFNC
Sbjct: 744 FLAKYCKGYTTAATFDLGGIGFAFPKGSTLAVDVSTSIIELIERRKMPQLETTLLSTFNC 803

Query: 722 SLSSQVDGSSSLGPWPFAGFLLLDGS 723
           SL SQVDGSSSLGPWPFAG  ++ GS
Sbjct: 804 SLGSQVDGSSSLGPWPFAGLFIVSGS 829

BLAST of Lsi08G014760 vs. NCBI nr
Match: KAA0060906.1 (glutamate receptor 2.5-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1240.3 bits (3208), Expect = 0.0e+00
Identity = 654/851 (76.85%), Postives = 716/851 (84.14%), Query Frame = 0

Query: 1    MSPLDLISIKEVSTILGAFTLQEMQLMSEINKTFIDIPIISLPVAASIPP--NGLFPPPS 60
            +S LDLI  KEVSTILGAFT QEMQLMSEIN  FIDIPIISLP+AAS+ P  N LFP PS
Sbjct: 1211 ISALDLIGNKEVSTILGAFTSQEMQLMSEINTNFIDIPIISLPIAASLTPHNNNLFPHPS 1270

Query: 61   FIQMAHHITFHMQCTAALVGHFQWHKVTVIYEHTNDMSFNMEALTLLSNQLGVFHAEIEQ 120
            FIQMA +ITFH+QCTAA+V HFQWHKVT+IY+ TNDMSFNMEALTLLSNQLG F+ EI+Q
Sbjct: 1271 FIQMAQNITFHIQCTAAVVAHFQWHKVTLIYDITNDMSFNMEALTLLSNQLGAFNVEIDQ 1330

Query: 121  ISSFSSSYTEAMIEEKLKNLVGHERNKVFIVVQFSIELAKLLFHKANKMKMMENGFVWIV 180
            ISSFSSSYTE+MIEEKLK+LVG ERNKVFI+VQFSIELAKLLFHKA +M MM+NGFVWIV
Sbjct: 1331 ISSFSSSYTESMIEEKLKSLVGRERNKVFILVQFSIELAKLLFHKAKEMNMMDNGFVWIV 1390

Query: 181  GDEISSHLDSLDSSTFNDMQGVIGFRTYFDHNKDSFKKFRTKFLRKYVLEYHHQEEEMKN 240
            GDEISSHLDSL SSTFNDMQGVIGFRTYFD NKDSFKKFR+KF RKYV EY  ++EEM N
Sbjct: 1391 GDEISSHLDSLGSSTFNDMQGVIGFRTYFDRNKDSFKKFRSKFQRKYVSEYDDEDEEMTN 1450

Query: 241  GEPSIFALRAYDASWAVALAMNKLQGNFTNKQLLKEILGSEFEGLSGKIGFKNGALKEPP 300
            GEP+IFALRAYDA  AVALA++KLQ NF+NKQLLKEIL  EFEGLSGKIG KNG L EPP
Sbjct: 1451 GEPTIFALRAYDAGCAVALAVHKLQANFSNKQLLKEILRIEFEGLSGKIGVKNGVLMEPP 1510

Query: 301  TFEIIYVVGKSYKEMGFWRQKVGFF-SMIENDEE--ISSIIIDERRSRS--------NNN 360
            TFEIIYVVGKSYK MGFWR+KVGFF +MIEN+++   SSIII   RSRS        NNN
Sbjct: 1511 TFEIIYVVGKSYKGMGFWREKVGFFNNMIENNDQEMSSSIIIHGGRSRSSNNNDDNNNNN 1570

Query: 361  GVLELPRFIFLEGNAKTGLIKGRINVDNSDSRVIGRTLRIGVPANNTFREFVKVSYNHIN 420
             VLELPRF+  EGNA TGLIK RI+V+NS+  V GR L+IGVPANNTF++FV+V Y+H+N
Sbjct: 1571 VVLELPRFVLWEGNAGTGLIKRRIDVENSNFGVTGRILKIGVPANNTFQDFVRVCYSHLN 1630

Query: 421  GMYISGFSITVFEAVAKNLPYPLSYQLVPFNGSYDGLVKQVYEKDLDAAVGDIGIFADRF 480
            GMYISGFSITVFEAVAKNLPYPL YQLVPFNGSYDGL++QVY K LD AVGDIGI ADRF
Sbjct: 1631 GMYISGFSITVFEAVAKNLPYPLLYQLVPFNGSYDGLIEQVYTKGLDGAVGDIGIIADRF 1690

Query: 481  QYVDFTEPYLVSGLLLIVKEETENWKEIWVFMKTFTTTMWLILPFSHIFIISIVWLVKEE 540
            +YVDFTEPYLVSGLL+IVKEET+ WKEIW FM+TFTTTMW+ILP SHIFIIS+VWLVKE+
Sbjct: 1691 RYVDFTEPYLVSGLLMIVKEETKIWKEIWAFMRTFTTTMWIILPISHIFIISVVWLVKED 1750

Query: 541  -SEDLSGFGEMLWFSVTVIFYTQRKEVKGFLARLVLGTWLFVILVVTSSFTASLTSMMTV 600
             S+DLSGFGEMLWFS+TVIFY Q++EVKGFLARLVLGTWLFVILVVTSSFTASLTSMMTV
Sbjct: 1751 SSDDLSGFGEMLWFSITVIFYAQKREVKGFLARLVLGTWLFVILVVTSSFTASLTSMMTV 1810

Query: 601  SRFAPSVVDIETIRQTNATVGCNFHSFIIRYLNDVLHIPSANIKTLVGIDDYPKAFDNGE 660
            SRFAPSVVDIET+RQ NATVGCNF+SFIIRYLNDVL IP ANIKTL G+D+YPKAFDNG+
Sbjct: 1811 SRFAPSVVDIETLRQMNATVGCNFNSFIIRYLNDVLKIPLANIKTLSGLDEYPKAFDNGQ 1870

Query: 661  IQAAFFITPHAK-----------------------AFPKGSTLAVDVSTSIIELIERRKM 720
            I+AAFFITPHAK                       AFPKGS+LAVDVSTSIIELIERRKM
Sbjct: 1871 IEAAFFITPHAKVFLAKYCRGYTTAATFDLGGLGFAFPKGSSLAVDVSTSIIELIERRKM 1930

Query: 721  PQLETTLLSTFNCSLSSQVDGSSSLGPWPFAGFLLLDGSSSKNDGKFRCSSDDPKTVLNI 780
            PQLETTLLSTFNCS SSQVDGSSSLGPWPFAGFLLL+GS SKNDG F+CSSD+PKTVLNI
Sbjct: 1931 PQLETTLLSTFNCSPSSQVDGSSSLGPWPFAGFLLLNGSFSKNDGNFQCSSDEPKTVLNI 1990

Query: 781  GVIADNRSRVGREHIIAIEM------------------------------GLDLISNKEV 785
            GVIAD+ SR GREHIIAI+M                               LDLISNKEV
Sbjct: 1991 GVIADSSSRAGREHIIAIQMAVEDYIFTSCHKVELLLVDSPENSAQTTATSLDLISNKEV 2050

BLAST of Lsi08G014760 vs. NCBI nr
Match: XP_008444616.1 (PREDICTED: glutamate receptor 2.1-like [Cucumis melo])

HSP 1 Score: 1155.6 bits (2988), Expect = 0.0e+00
Identity = 601/758 (79.29%), Postives = 658/758 (86.81%), Query Frame = 0

Query: 1   MSPLDLISIKEVSTILGAFTLQEMQLMSEINKTFIDIPIISLPVAASIPP--NGLFPPPS 60
           +S LDLI  KEVSTILGAFT QEMQLMSEIN  FIDIPIISLP+AAS+ P  N LFP PS
Sbjct: 94  ISALDLIGNKEVSTILGAFTSQEMQLMSEINTNFIDIPIISLPIAASLTPHNNNLFPHPS 153

Query: 61  FIQMAHHITFHMQCTAALVGHFQWHKVTVIYEHTNDMSFNMEALTLLSNQLGVFHAEIEQ 120
           FIQMA +ITFH+QCTAA+V HFQWHKVT+IY+ TNDMSFNMEALTLLSNQLG F+ EI+Q
Sbjct: 154 FIQMAQNITFHIQCTAAVVAHFQWHKVTLIYDITNDMSFNMEALTLLSNQLGAFNVEIDQ 213

Query: 121 ISSFSSSYTEAMIEEKLKNLVGHERNKVFIVVQFSIELAKLLFHKANKMKMMENGFVWIV 180
           ISSFSSSYTE+MIEEKLK+LVG ERNKVFI+VQFSIELAKLLFHKA +M MM+NGFVWIV
Sbjct: 214 ISSFSSSYTESMIEEKLKSLVGRERNKVFILVQFSIELAKLLFHKAKEMNMMDNGFVWIV 273

Query: 181 GDEISSHLDSLDSSTFNDMQGVIGFRTYFDHNKDSFKKFRTKFLRKYVLEYHHQEEEMKN 240
           GDEISSHLDSL SSTFNDMQGVIGFRTYFD NKDSFKKFR+KF RKYV EY  ++EEM N
Sbjct: 274 GDEISSHLDSLGSSTFNDMQGVIGFRTYFDRNKDSFKKFRSKFQRKYVSEYDDEDEEMTN 333

Query: 241 GEPSIFALRAYDASWAVALAMNKLQGNFTNKQLLKEILGSEFEGLSGKIGFKNGALKEPP 300
           GEP+IFALRAYDA WAVALA++KLQ NF+NKQLLKEIL  EFEGLSGKIG KNG L EPP
Sbjct: 334 GEPTIFALRAYDAGWAVALAVHKLQANFSNKQLLKEILRIEFEGLSGKIGVKNGVLMEPP 393

Query: 301 TFEIIYVVGKSYKEMGFWRQKVGFF-SMIENDEE--ISSIIIDERRSRS-------NNNG 360
           TFEIIYVVGKSYK MGFWR+KVGFF +MIEN+++   SSIII   RSRS       NNN 
Sbjct: 394 TFEIIYVVGKSYKGMGFWREKVGFFNNMIENNDQEMSSSIIIHGGRSRSSNNNDDNNNNV 453

Query: 361 VLELPRFIFLEGNAKTGLIKGRINVDNSDSRVIGRTLRIGVPANNTFREFVKVSYNHING 420
           VLELPRF+  EGNA TGLIK RI+V+NS+  V GR L+IGVPANNTF++FV+V Y+H+NG
Sbjct: 454 VLELPRFVLWEGNAGTGLIKRRIDVENSNFGVTGRILKIGVPANNTFQDFVRVCYSHLNG 513

Query: 421 MYISGFSITVFEAVAKNLPYPLSYQLVPFNGSYDGLVKQVYEKDLDAAVGDIGIFADRFQ 480
           MYISGFSITVFEAVAKNLPYPL YQLVPFNGSYDGL++QVY K LD AVGDIGI ADRF+
Sbjct: 514 MYISGFSITVFEAVAKNLPYPLLYQLVPFNGSYDGLIEQVYTKGLDGAVGDIGIIADRFR 573

Query: 481 YVDFTEPYLVSGLLLIVKEETENWKEIWVFMKTFTTTMWLILPFSHIFIISIVWLVKEE- 540
           YVDFTEPYLVSGLL+IVKEET+ WKEIW FM+TFTTTMW+ILP SHIFIIS+VWLVKE+ 
Sbjct: 574 YVDFTEPYLVSGLLMIVKEETKIWKEIWAFMRTFTTTMWIILPISHIFIISVVWLVKEDS 633

Query: 541 SEDLSGFGEMLWFSVTVIFYTQRKEVKGFLARLVLGTWLFVILVVTSSFTASLTSMMTVS 600
           S+DLSGFGEMLWFS+TVIFY Q++EVKGFLARLVLGTWLFVILVVTSSFTASLTSMMTVS
Sbjct: 634 SDDLSGFGEMLWFSITVIFYAQKREVKGFLARLVLGTWLFVILVVTSSFTASLTSMMTVS 693

Query: 601 RFAPSVVDIETIRQTNATVGCNFHSFIIRYLNDVLHIPSANIKTLVGIDDYPKAFDNGEI 660
           RFAPSVVDIET+RQ NATVGCNF+SFIIRYLNDVL IP ANIKTL G+D+YPKAFDNG+I
Sbjct: 694 RFAPSVVDIETLRQMNATVGCNFNSFIIRYLNDVLKIPLANIKTLSGLDEYPKAFDNGQI 753

Query: 661 QAAFFITPHAK-----------------------AFPKGSTLAVDVSTSIIELIERRKMP 720
           +AAFFITPHAK                       AFPKGS+LAVDVSTSIIELIERRKMP
Sbjct: 754 EAAFFITPHAKVFLAKYCRGYTTAATFDLGGLGFAFPKGSSLAVDVSTSIIELIERRKMP 813

Query: 721 QLETTLLSTFNCSLSSQVDGSSSLGPWPFAGFLLLDGS 723
           QLETTLLSTFNCS SSQVDGSSSLGPWPFAG   L GS
Sbjct: 814 QLETTLLSTFNCSPSSQVDGSSSLGPWPFAGLFFLSGS 851

BLAST of Lsi08G014760 vs. NCBI nr
Match: XP_031737055.1 (glutamate receptor 2.1 [Cucumis sativus] >KGN62775.2 hypothetical protein Csa_021808 [Cucumis sativus])

HSP 1 Score: 1123.2 bits (2904), Expect = 0.0e+00
Identity = 584/775 (75.35%), Postives = 657/775 (84.77%), Query Frame = 0

Query: 2   SPLDLISIKEVSTILGAFTLQEMQLMSEINKTFIDIPIISLPVAASIPP---NGLFPPPS 61
           S LDLI  KEVSTILGAFTLQEMQLMSEINK FIDI IISLP+AAS+PP   N   P PS
Sbjct: 90  SALDLIGNKEVSTILGAFTLQEMQLMSEINKNFIDISIISLPIAASLPPHNNNNPLPLPS 149

Query: 62  FIQMAHHITFHMQCTAALVGHFQWHKVTVIYEHTNDMSFNMEALTLLSNQLGVFHAEIEQ 121
           FI+MAH+ITFH+QCTAA+V HFQWHKVT+IY++T+DMSFNMEALTLLSNQLG F+ EI+Q
Sbjct: 150 FIRMAHNITFHIQCTAAIVAHFQWHKVTLIYDNTDDMSFNMEALTLLSNQLGDFNIEIDQ 209

Query: 122 ISSFSSSYTEAMIEEKLKNLVGHERNKVFIVVQFSIELAKLLFHKANKMKMMENGFVWIV 181
           ISSFSSSY+E+MIEEKLK+LVG ERN+VFI+VQFSIELAKLLFHKANKM MMENGFVWIV
Sbjct: 210 ISSFSSSYSESMIEEKLKSLVGRERNQVFILVQFSIELAKLLFHKANKMNMMENGFVWIV 269

Query: 182 GDEISSHLDSLDSSTFNDMQGVIGFRTYFDHNKDSFKKFRTKFLRKYVLEYH-HQEEEMK 241
           GDEISSHLDSLDSSTFNDMQGVIGFRTYFDHNK+SFKKFR+KF RKYVLEYH ++EEEMK
Sbjct: 270 GDEISSHLDSLDSSTFNDMQGVIGFRTYFDHNKNSFKKFRSKFHRKYVLEYHENEEEEMK 329

Query: 242 NGEPSIFALRAYDASWAVALAMNKLQGNFTNKQLLKEILGSEFEGLSGKIGFKNGALKEP 301
           N EP+IFALRAYDA WAVALAM+KLQ NF+NKQLLKEIL S+FEGLSGKIGFKNG L EP
Sbjct: 330 NTEPTIFALRAYDAGWAVALAMHKLQANFSNKQLLKEILRSKFEGLSGKIGFKNGVLMEP 389

Query: 302 PTFEIIYVVGKSYKEMGFWRQKVGFFSMI--ENDEEI-SSIIIDERRSRS---------- 361
           PTFEIIYVVGKSYKE+GFWR+KVGFF  +   ND+EI SSIIIDE RSRS          
Sbjct: 390 PTFEIIYVVGKSYKEIGFWREKVGFFINLNENNDQEISSSIIIDEGRSRSDNNNNNNNNN 449

Query: 362 -NNNGVLELPRFIFLEGN-AKTGLIKGR-INVDNSDSRVIGRTLRIGVPANNTFREFVKV 421
            NNN VL+LPRF+  E N A+TG++KGR IN+DNS+S  +GRTLRIG+PANNTFREFVKV
Sbjct: 450 NNNNVVLKLPRFVLWEVNYAETGVVKGRTINIDNSNSGGMGRTLRIGIPANNTFREFVKV 509

Query: 422 SYNHINGMYISGFSITVFEAVAKNLPYPLSYQLVPFNGSYDGLVKQVYEKDLDAAVGDIG 481
           SY+HIN +YISGFSI+VFEAV KNLPY L YQL+P NGSYDGLVKQVY + LDAAVGDIG
Sbjct: 510 SYDHINAIYISGFSISVFEAVVKNLPYSLPYQLIPINGSYDGLVKQVYTRGLDAAVGDIG 569

Query: 482 IFADRFQYVDFTEPYLVSGLLLIVKEETENWKEIWVFMKTFTTTMWLILPFSHIFIISIV 541
           IFADRF+YVDFTEPY++ GL++IVKE+T NWKEIW+FMKTFTT MW+ILP  H+ I+S+V
Sbjct: 570 IFADRFKYVDFTEPYMMGGLVMIVKEKTRNWKEIWIFMKTFTTLMWIILPIFHLVIMSVV 629

Query: 542 WLVKEESE-DLSGFGEMLWFSVTVIFYTQRKEVKGFLARLVLGTWLFVILVVTSSFTASL 601
           W+VK+  + +LSG  EM+WF+VTVIF+  RKEVKG LARLVLGTWLFVILVVTSSFTASL
Sbjct: 630 WIVKDPKDGELSGVSEMIWFAVTVIFFAHRKEVKGNLARLVLGTWLFVILVVTSSFTASL 689

Query: 602 TSMMTVSRFAPSVVDIETIRQTNATVGCNFHSFIIRYLNDVLHIPSANIKTLVGIDDYPK 661
           TSMMTVSRFAPSVVD+ET+RQ NATVGCN+HSFI RYLND L IP  NIK  VGIDDYPK
Sbjct: 690 TSMMTVSRFAPSVVDVETLRQMNATVGCNYHSFIPRYLNDTLKIPRINIKNFVGIDDYPK 749

Query: 662 AFDNGEIQAAFFITPHAK-----------------------AFPKGSTLAVDVSTSIIEL 721
           AFDNGEI+AAFFITPHAK                       AF KGS+LAVDVSTSI+EL
Sbjct: 750 AFDNGEIEAAFFITPHAKVFLARYCKGYITAATFNLGGIGFAFRKGSSLAVDVSTSIVEL 809

Query: 722 IERRKMPQLETTLLSTFNCSLSSQVDGSSSLGPWPFAGFLLLDGSSSKNDGKFRC 733
           IERR+MPQLET LLSTFNCS  SQVDGS+SLGPWPFAG  ++  S +     + C
Sbjct: 810 IERREMPQLETMLLSTFNCSSGSQVDGSTSLGPWPFAGLFIISASVAAGSLLYFC 864

BLAST of Lsi08G014760 vs. TAIR 10
Match: AT5G27100.1 (glutamate receptor 2.1 )

HSP 1 Score: 258.8 bits (660), Expect = 1.4e-68
Identity = 213/743 (28.67%), Postives = 360/743 (48.45%), Query Frame = 0

Query: 4   LDLISIKEVSTILGAFTLQEMQLMSEINKTFIDIPIISLPVAASIPPNGLFPPPSFIQMA 63
           LDLI+ KEV  ILG +T  + Q M E+ +    +PI++   +A+ P         F +  
Sbjct: 89  LDLITNKEVKAILGPWTSMQAQFMIEMGQK-SQVPIVT--YSATSPSLASIRSQYFFRAT 148

Query: 64  HHITFHMQCTAALVGHFQWHKVTVIYEHTNDMSFNMEALTLLSNQLGVFHAEIEQISSFS 123
           +  +  +     ++  F W +V  +Y    D +F    +  L++ L   +  I   +  S
Sbjct: 149 YDDSSQVHAIKEIIKLFGWREVAPVYV---DDTFGEGIMPRLTDVLQEINVRIPYRTVIS 208

Query: 124 SSYTEAMIEEKLKNLVGHERNKVFIVVQFSIELAKLLFHKANKMKMMENGFVWIVGDEIS 183
            + T+  I  +L  ++     +VF+V    + LA   F KA ++ +M+ G+VWI+ + I+
Sbjct: 209 PNATDDEISVELLRMMTLP-TRVFVVHLVEL-LASRFFAKATEIGLMKQGYVWILTNTIT 268

Query: 184 SHLDSLDSSTFNDMQGVIGFRTYFDHNKDSFKKFRTKFLRKYVLEYHHQEEEMKNGEPSI 243
             L  ++ +    MQGV+G +TY   +K+  + FR+++ +++ +            + ++
Sbjct: 269 DVLSIMNETEIETMQGVLGVKTYVPRSKE-LENFRSRWTKRFPI-----------SDLNV 328

Query: 244 FALRAYDASWAVALA-------------------MNKLQGNFTNK---QLLKEILGSEFE 303
           + L AYDA+ A+ALA                   +++LQG   ++   +LL+ +    F+
Sbjct: 329 YGLWAYDATTALALAIEEAGTSNLTFVKMDAKRNVSELQGLGVSQYGPKLLQTLSRVRFQ 388

Query: 304 GLSGKIGFKNGALKEPPTFEIIYVVGKSYKEMGFWRQKVGFFSMIENDEEISSIIIDERR 363
           GL+G   F NG L +P  FEI+ V G+  + +GFW ++ G F  ++           +  
Sbjct: 389 GLAGDFQFINGEL-QPSVFEIVNVNGQGGRTIGFWMKEYGLFKNVD----------QKPA 448

Query: 364 SRSNNNGVLELPRFIFLEGNAKTGLIKGRINVDNSDSRVIGRTLRIGVPANNTFREFVKV 423
           S++  +   +  R I   G+  T + KG     N      G+ L+IGVP NNTF++FVK 
Sbjct: 449 SKTTFSSWQDRLRPIIWPGDT-TSVPKGWEIPTN------GKRLQIGVPVNNTFQQFVKA 508

Query: 424 SYNHI-NGMYISGFSITVFEAVAKNLPYPLSYQLVPF-NGSYDGLVKQVYEKDLDAAVGD 483
           + + I N    SGFSI  FEAV + +PY +SY  +PF +G YD LV QVY    DA V D
Sbjct: 509 TRDPITNSTIFSGFSIDYFEAVIQAIPYDISYDFIPFQDGGYDALVYQVYLGKYDAVVAD 568

Query: 484 IGIFADRFQYVDFTEPYLVSGLLLIVKEETENWKEIWVFMKTFTTTMWLILPFSHIFIIS 543
             I ++R  YVDF+ PY  SG+ L+V  +    +   +F+   T  +WLI   S   I  
Sbjct: 569 TTISSNRSMYVDFSLPYTPSGVGLVVPVKDSVRRSSTIFLMPLTLALWLISLLSFFIIGL 628

Query: 544 IVWLVKEE-SEDLSGFGE-----MLWFSVTVIFYTQRKEVKGFLARLVLGTWLFVILVVT 603
           +VW+++   + D  G G+     + WFS +++ +  R+ V  F AR+V+  W F++LV+T
Sbjct: 629 VVWVLEHRVNPDFDGPGQYQLSTIFWFSFSIMVFAPRERVLSFWARVVVIIWYFLVLVLT 688

Query: 604 SSFTASLTSMMTVSRFAPSVVDIETIRQTNATVGCNFHSFIIRYLNDVLHIPSANIKTLV 663
            S+TASL S++T     P+V +I ++     +VG    SFI+  L D     ++ +    
Sbjct: 689 QSYTASLASLLTTQHLHPTVTNINSLLAKGESVGYQ-SSFILGRLRDSGFSEASLVS--Y 748

Query: 664 GIDDY-----PKAFDNGEIQAAFFITPHAK------------------------AFPKGS 688
           G  ++      K    G + A     P+ +                         FP GS
Sbjct: 749 GSPEHCDALLSKGQAEGGVSAVLMEVPYVRIFLGQYCNKYKMVQTPFKVDGLGFVFPIGS 790

BLAST of Lsi08G014760 vs. TAIR 10
Match: AT5G11210.1 (glutamate receptor 2.5 )

HSP 1 Score: 249.6 bits (636), Expect = 8.8e-66
Identity = 212/782 (27.11%), Postives = 357/782 (45.65%), Query Frame = 0

Query: 10  KEVSTILGAFTLQEMQLMSEINKTFIDIPIISLPVAASIPPNGLFPPPSFIQMAHHITFH 69
           +EV  I+G  T  +   +  +      +PIIS   +A+ P       P FI+  H  +  
Sbjct: 26  REVVAIIGPGTSMQAPFLINLGNQ-SKVPIISF--SATSPLLDSLRSPYFIRATHDDSSQ 85

Query: 70  MQCTAALVGHFQWHKVTVIYEHTNDMSFNMEALTLLSNQLGVFHAEIEQISSFSSSYTEA 129
           +Q  +A++  F+W +V  IY    D  F    L  L +     +  I   S+ S  Y++ 
Sbjct: 86  VQAISAIIESFRWREVVPIYV---DNEFGEGILPNLVDAFQEINVRIRYRSAISLHYSDD 145

Query: 130 MIEEKLKNLVGHERNKVFIVVQFSIELAKLLFHKANKMKMMENGFVWIVGDEISSHLDSL 189
            I+++L  L+     +VFIV     +L   LF  A ++ M+  G+VWIV + I+  +  +
Sbjct: 146 QIKKELYKLMTMP-TRVFIVHMLP-DLGSRLFSIAKEIDMLSKGYVWIVTNGIADLMSIM 205

Query: 190 DSSTFNDMQGVIGFRTYFDHNKDSFKKFRTKFLRKYVLEYHHQEEEMKNGEPSIFALRAY 249
             S+  +M GV+G +TYF  +K+       ++ +++        EE+ N     FA  AY
Sbjct: 206 GESSLVNMHGVLGVKTYFAKSKE-LLHLEARWQKRF------GGEELNN-----FACWAY 265

Query: 250 DASWAVALAMNKLQG-----NFTNKQLLKEILGSE---------------------FEGL 309
           DA+ A+A+++ +++      N T +   ++ +G++                     F+G+
Sbjct: 266 DAATALAMSVEEIRHVNMSFNTTKEDTSRDDIGTDLDELGVALSGPKLLDALSTVSFKGV 325

Query: 310 SGKIGFKNGALKEPPTFEIIYVVGKSYKEMGFWRQKVGFFSMIENDEEISSIIIDERRSR 369
           +G+   KNG L E  TF+II +     + +GFW+ KVG    +  D+   S     RR R
Sbjct: 326 AGRFQLKNGKL-EATTFKIINIEESGERTVGFWKSKVGLVKSLRVDKVSHS----SRRLR 385

Query: 370 SN--NNGVLELPRFIFLEGNAKTGLIKGRINVDNSDSRVIGRTLRIGVPANNTFREFVKV 429
                   + +P+      NAK                     LRI VP  + F  FV+V
Sbjct: 386 PIIWPGDTIFVPKGWEFPTNAK--------------------KLRIAVPKKDGFNNFVEV 445

Query: 430 SYN-HINGMYISGFSITVFEAVAKNLPYPLSYQLVPFN-------GSYDGLVKQVYEKDL 489
           + + + N   ++GF I VF  V   +PY +SY+ +PF+       GSYD +V  V+  + 
Sbjct: 446 TKDENTNVPTVTGFCIDVFNTVMSQMPYAVSYEYIPFDTPDGKPRGSYDEMVYNVFLGEF 505

Query: 490 DAAVGDIGIFADRFQYVDFTEPYLVSGLLLIVKEETENWKEIWVFMKTFTTTMWLILPFS 549
           D AVGD  I A+R  YVDF  PY  +G++ +V  +    K  WVF+K  T  +WL+   S
Sbjct: 506 DGAVGDTTILANRSHYVDFALPYSETGIVFLVPVKDGKEKGEWVFLKPLTKELWLVTAAS 565

Query: 550 HIFIISIVWLVKEESED-------LSGFGEMLWFSVTVIFYTQRKEVKGFLARLVLGTWL 609
            ++I  +VW+ + ++++       +     + +FS + +F+  R+  + F  R+++  W 
Sbjct: 566 FLYIGIMVWIFEYQADEEFREQMIIDKISSVFYFSFSTLFFAHRRPSESFFTRVLVVVWC 625

Query: 610 FVILVVTSSFTASLTSMMTVSRFAPSVVDIETIRQTNATVGCNFHSFIIRYLNDVLHIPS 669
           FV+L++T S+TA+LTSM+TV    P+V  ++ +R++   +G    SF    L   +    
Sbjct: 626 FVLLILTQSYTATLTSMLTVQELRPTVRHMDDLRKSGVNIGYQTGSFTFERLKQ-MRFDE 685

Query: 670 ANIKTLVGIDDYPKAF----DNGEIQAAF------------------FITPHAK------ 720
           + +KT    ++  + F     NG I AAF                   I P  K      
Sbjct: 686 SRLKTYNSPEEMRELFLHKSSNGGIDAAFDEVAYIKLFMAKYCSEYSIIEPTFKADGFGF 745

BLAST of Lsi08G014760 vs. TAIR 10
Match: AT2G24720.1 (glutamate receptor 2.2 )

HSP 1 Score: 246.5 bits (628), Expect = 7.4e-65
Identity = 214/747 (28.65%), Postives = 354/747 (47.39%), Query Frame = 0

Query: 4   LDLISIKEVSTILGAFTLQEMQLMSEINKTFIDIPIISLPVAASIPPNGLFPPPSFIQMA 63
           +DLI  K+V  ILG +T  +   + EI +    +P++S   +A+ P       P F +  
Sbjct: 89  IDLIKNKQVKAILGPWTSMQAHFLIEIGQK-SRVPVVS--YSATSPSLTSLRSPYFFRAT 148

Query: 64  HHITFHMQCTAALVGHFQWHKVTVIYEHTNDMSFNMEALTLLSNQLGVFHAEIEQISSFS 123
           +  +  +    A++  F W +V  +Y    D +F    +  L++ L   +  I   S   
Sbjct: 149 YEDSSQVHAIKAIIKLFGWREVVPVY---IDNTFGEGIMPRLTDSLQDINVRIPYRSVIP 208

Query: 124 SSYTEAMIEEKLKNLVGHERNKVFIVVQFSIELAKLLFHKANKMKMMENGFVWIVGDEIS 183
            + T+  I  +L  ++ +   +VFI V  S  LA  +F KA ++ +M+ G+VWI+ + + 
Sbjct: 209 LNATDQDISVELLKMM-NMPTRVFI-VHMSSSLASTVFIKAKELGLMKPGYVWILTNGVM 268

Query: 184 SHLDSLDSSTFNDMQGVIGFRTYFDHNKDSFKKFRTKFLRKYVLEYHHQEEEMKNGEPSI 243
             L S++ +    M+GV+G +TY   +KD  + FR+++ R++              E ++
Sbjct: 269 DGLRSINETGIEAMEGVLGIKTYIPKSKD-LETFRSRWKRRF-----------PQMELNV 328

Query: 244 FALRAYDASWAVALAMNKLQGN---FTN-------------------KQLLKEILGSEFE 303
           + L AYDA+ A+A+A+     N   F+N                    +LL+ +   +F+
Sbjct: 329 YGLWAYDATTALAMAIEDAGINNMTFSNVDTGKNVSELDGLGLSQFGPKLLQTVSTVQFK 388

Query: 304 GLSGKIGFKNGALKEPPTFEIIYVVGKSYKEMGFWRQKVGFFSMIENDEEISSIIIDERR 363
           GL+G   F +G L +P  FEI+ ++G   + +GFW +  G    ++           E R
Sbjct: 389 GLAGDFHFVSGQL-QPSVFEIVNMIGTGERSIGFWTEGNGLVKKLD----------QEPR 448

Query: 364 SRSNNNGVLELPRFIFLEGNAKTGLIKGRINVDNSDSRVIGRTLRIGVPANNTFREFVKV 423
           S    +   +  + I   G A   + KG     N      G+ LRIGVP    F + VKV
Sbjct: 449 SIGTLSTWPDHLKHIIWPGEA-VSVPKGWEIPTN------GKKLRIGVPKRIGFTDLVKV 508

Query: 424 SYNHI-NGMYISGFSITVFEAVAKNLPYPLSYQLVPFN-------GSYDGLVKQVYEKDL 483
           + + I N   + GF I  FEAV + +PY +SY+  PF        G+++ LV QVY    
Sbjct: 509 TRDPITNSTVVKGFCIDFFEAVIQAMPYDVSYEFFPFEKPNGEPAGNHNDLVHQVYLGQF 568

Query: 484 DAAVGDIGIFADRFQYVDFTEPYLVSGLLLIVKEETENWKEIWVFMKTFTTTMWL-ILPF 543
           DA VGD  I A+R  +VDFT P++ SG+ LIV  + E  ++ + F+K  +  +WL  L F
Sbjct: 569 DAVVGDTTILANRSSFVDFTLPFMKSGVGLIVPLKDEVKRDKFSFLKPLSIELWLTTLVF 628

Query: 544 SHIFIISIVWLVKEESEDLSG-----FGEMLWFSVTVIFYTQRKEVKGFLARLVLGTWLF 603
             +  IS+  L    + D  G        + WF+ + + +  R+ V  F AR ++ TW F
Sbjct: 629 FFLVGISVWTLEHRVNSDFRGPANYQASTIFWFAFSTMVFAPRERVLSFGARSLVVTWYF 688

Query: 604 VILVVTSSFTASLTSMMTVSRFAPSVVDIETIRQTNATVGCNFHSFIIRYLNDVLHIPSA 663
           V+LV+T S+TASL S++T  +  P++  + ++     TVG    SFI+  LN+     S+
Sbjct: 689 VLLVLTQSYTASLASLLTSQQLNPTITSMSSLLHRGETVGYQRTSFILGKLNETGFPQSS 748

Query: 664 NI--KTLVGIDD-YPKAFDNGEIQAAFFITPHAK------------------------AF 688
            +   T    D+   K   NG + AAF  TP+ +                         F
Sbjct: 749 LVPFDTAEECDELLKKGPKNGGVAAAFLGTPYVRLFLGQYCNTYKMVEEPFNVDGFGFVF 797

BLAST of Lsi08G014760 vs. TAIR 10
Match: AT2G17260.1 (glutamate receptor 2 )

HSP 1 Score: 246.1 bits (627), Expect = 9.7e-65
Identity = 222/786 (28.24%), Postives = 363/786 (46.18%), Query Frame = 0

Query: 11  EVSTILGAFTLQEMQLMSEINKTFIDIPIISLPVAASIPPNGLFPPPSFIQMAHHITFHM 70
           +V  I+G  T     ++S +    + +P++S    A  P       P F+Q A    F M
Sbjct: 124 DVVAIIGPQTSIMAHVLSHLANE-LTVPMLSF--TALDPTLSPLQFPFFVQTAPSDLFLM 183

Query: 71  QCTAALVGHFQWHKVTVIYEHTNDMSFNMEALTLLSNQL-----GVFHAEIEQISSFSSS 130
           +  A ++ ++ W  V  +Y   ND   +   +T L ++L      + +  +  +    +S
Sbjct: 184 RAIAEMITYYGWSDVVALY---NDDDNSRNGVTALGDELEERRCKISYKAVLPLDVVITS 243

Query: 131 YTEAMIEEKLKNLVGHERNKVFIVVQFSIELAKLLFHKANKMKMMENGFVWIVGDEISSH 190
             E +IEE +K  +    ++V +V  F     K++F +A ++ MME G+VWI    +SS 
Sbjct: 244 PVE-IIEELIK--IRGMESRVIVVNTFP-NTGKMIFKEAERLGMMEKGYVWIATTWLSSV 303

Query: 191 LDS---LDSSTFNDMQGVIGFRTYFDHNKDSFKKFRTKFLRKYVLEYHHQEEEMKNGEPS 250
           LDS   LD+   N   GV+  R    H  DS KK      R +   + ++    K    +
Sbjct: 304 LDSNLPLDTKLVN---GVLTLRL---HTPDSRKK------RDFAARWKNKLSNNKTIGLN 363

Query: 251 IFALRAYDASWAVALA------------------MNKLQGNFTN----------KQLLKE 310
           ++ L AYD  W +A A                  +  L+G   N           QLL  
Sbjct: 364 VYGLYAYDTVWIIARAVKTLLEAGGNLSFSNDAKLGSLKGEALNLSALSRFDQGSQLLDY 423

Query: 311 ILGSEFEGLSGKIGFKNGALKEPPTFEIIYVVGKSYKEMGFWRQKVGFFSMIENDEEISS 370
           I+ ++  GL+G + F        P+++II +V     ++G+W    G  S++  +   S 
Sbjct: 424 IVHTKMSGLTGPVQFHPDRSMLQPSYDIINLVDDRVHQIGYWSNYSG-LSIVPPESFYSK 483

Query: 371 IIIDERRSRSNNNGVLELPRFIFLEGNAKTGLIKGRINVDNSDSRVIGRTLRIGVPANNT 430
                 RS SN +    L    +  G + T   +G I  +N      GR LRIGVP   +
Sbjct: 484 ---PPNRSSSNQH----LNSVTWPGGTSVTP--RGWIFRNN------GRRLRIGVPDRAS 543

Query: 431 FREFVKVSYNHINGM--YISGFSITVFEAVAKNLPYPLSYQLVPF-----NGSYDGLVKQ 490
           F++FV    + +NG    + G+ I VFEA  K L YP+ ++ + F     N +Y+ LV +
Sbjct: 544 FKDFV----SRVNGSSNKVQGYCIDVFEAAVKLLSYPVPHEFIFFGDGLTNPNYNELVNK 603

Query: 491 VYE-KDLDAAVGDIGIFADRFQYVDFTEPYLVSGLLLIVKEETENWKEIWVFMKTFTTTM 550
           V    D DA VGDI I   R + VDFT+PY+ SGL+++      N +  W F++ FT  M
Sbjct: 604 VTTGVDFDAVVGDIAIVTKRTRIVDFTQPYIESGLVVVAPVTRLN-ENPWAFLRPFTLPM 663

Query: 551 WLILPFSHIFIISIVWLVKEESED------LSGFGEMLWFSVTVIFYTQRKEVKGFLARL 610
           W +     + + + +W+++    D            +LWF+ + +F++ R+     L R+
Sbjct: 664 WAVTASFFVIVGAAIWILEHRINDEFRGPPRRQIITILWFTFSTMFFSHRETTVSTLGRM 723

Query: 611 VLGTWLFVILVVTSSFTASLTSMMTVSRFAPSVVDIETIRQTNATVGCNFHSFIIRYLND 670
           VL  WLFV+L++TSS+TASLTS++TV +    +  ++T+  +   +G    SF   Y+ D
Sbjct: 724 VLLIWLFVVLIITSSYTASLTSILTVQQLNSPIKGVDTLISSTGRIGFQVGSFAENYMTD 783

Query: 671 VLHIPSANIKTLVGIDDYPKAFDNGEIQAA--------FFITPHAK-------------- 722
            L+I S+ +  L   ++Y  A  NG + A          F++ + K              
Sbjct: 784 ELNIASSRLVPLASPEEYANALQNGTVAAIVDERPYIDLFLSDYCKFAIRGQEFTRCGWG 843

BLAST of Lsi08G014760 vs. TAIR 10
Match: AT2G24710.1 (glutamate receptor 2.3 )

HSP 1 Score: 245.4 bits (625), Expect = 1.7e-64
Identity = 210/752 (27.93%), Postives = 357/752 (47.47%), Query Frame = 0

Query: 1   MSPLDLISIKEVSTILGAFTLQEMQLMSEINKTFIDIPIISLPVAASIPPNGLFPPPSFI 60
           ++ LDLI  K+V  ILG +T  +   + EI +    +PI+S   +A+ P       P F+
Sbjct: 85  IAALDLIKNKQVKAILGPWTSMQAHFLIEIGQK-SRVPIVS--YSATSPILTSLRSPYFL 144

Query: 61  QMAHHITFHMQCTAALVGHFQWHKVTVIYEHTNDMSFNMEALTLLSNQLGVFHAEIEQIS 120
           +  +  +F +Q   A++  F W +V  +Y    D +F    +  L++ L   +  I   S
Sbjct: 145 RATYEDSFQVQPIKAIIKLFGWREVVPVY---IDNTFGEGIMPRLTDALQDINVRIPYRS 204

Query: 121 SFSSSYTEAMIEEKLKNLVGHERNKVFIVVQFSIELAKLLFHKANKMKMMENGFVWIVGD 180
             + + T+  I  +L  ++ +   +VF+V  +  +LA   F KA ++ +ME G+VWI+ +
Sbjct: 205 VIAINATDHEISVELLKMM-NMPTRVFLVHMY-YDLASRFFIKAKELGLMEPGYVWILTN 264

Query: 181 EISSHLDSLDSSTFNDMQGVIGFRTYFDHNKDSFKKFRTKFLRKYVLEYHHQEEEMKNGE 240
            +   L  ++ +    M+GV+G +TY   + D  +KFR+++   +              E
Sbjct: 265 GVIDDLSLINETAVEAMEGVLGIKTYIPKSPD-LEKFRSRWRSLF-----------PRVE 324

Query: 241 PSIFALRAYDASWAVALAMNKL-QGNFT----------------------NKQLLKEILG 300
            S++ L AYDA+ A+A+A+ +    N T                        +LL+ +L 
Sbjct: 325 LSVYGLWAYDATTALAVAIEEAGTNNMTFSKVVDTGRNVSELEALGLSQFGPKLLQTLLT 384

Query: 301 SEFEGLSGKIGFKNGALKEPPTFEIIYVVGKSYKEMGFWRQKVGFFSMIENDEEISSIII 360
            +F GL+G+  F  G L +P  FEI+ ++    K +GFW++  G    +  D++ SSI  
Sbjct: 385 VQFRGLAGEFRFFRGQL-QPSVFEIVNIINTGEKSIGFWKEGNGLVKKL--DQQASSI-- 444

Query: 361 DERRSRSNNNGVLELPRFIFLEGNAKTGLIKGRINVDNSDSRVIGRTLRIGVPANNTFRE 420
                 S  +   +  + I   G A +     +I          G+ LRIGVP    + +
Sbjct: 445 ------SALSTWKDHLKHIVWPGEADSVPKGWQIPTK-------GKKLRIGVPKRTGYTD 504

Query: 421 FVKVSYNHI-NGMYISGFSITVFEAVAKNLPYPLSYQLVPFN-------GSYDGLVKQVY 480
            VKV+ + I N   ++GF I  FEAV + LPY +SY+ +PF        G+Y+ LV QVY
Sbjct: 505 LVKVTRDPITNSTVVTGFCIDFFEAVIRELPYDVSYEFIPFEKPDGKTAGNYNDLVYQVY 564

Query: 481 EKDLDAAVGDIGIFADRFQYVDFTEPYLVSGLLLIVKEETENWKEIWVFMKTFTTTMWLI 540
               DA VGD  I  +R  YVDFT P++ SG+ LIV+      ++  +FMK  +  +WL 
Sbjct: 565 LGRYDAVVGDTTILVNRSSYVDFTFPFIKSGVGLIVEMTDPVKRDYILFMKPLSWKLWLT 624

Query: 541 LPFSHIFIISIVWLVK-EESEDLSG-----FGEMLWFSVTVIFYTQRKEVKGFLARLVLG 600
              S   +   VW+++ + + D SG        + WF+ + + +  R+ V  F AR ++ 
Sbjct: 625 SFISFFLVGCTVWVLEYKRNPDFSGPPRFQASTICWFAFSTMVFAPRERVFSFWARALVI 684

Query: 601 TWLFVILVVTSSFTASLTSMMTVSRFAPSVVDIETIRQTNATVGCNFHSFIIRYLNDVLH 660
            W F++LV+T S+TASL S++T  +  P++  + ++ +   TVG    SFI+  L +   
Sbjct: 685 AWYFLVLVLTQSYTASLASLLTSQKLNPTITSMSSLLEKGETVGYQRTSFILGKLKE-RG 744

Query: 661 IPSANI---KTLVGIDD-YPKAFDNGEIQAAFFITPHAK--------------------- 688
            P +++    T    D+   K    G +  AF   P+ +                     
Sbjct: 745 FPQSSLVPFDTAEECDELLSKGPKKGGVSGAFLEIPYLRLFLGQFCNTYKMVEEPFNVDG 797