Lsi08G014600 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi08G014600
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	calmodulin-like
Location	chr08: 22747859 .. 22750912 (-)
RNA-Seq Expression	Lsi08G014600
Synteny	Lsi08G014600

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGGCCTCCCAACTTCAAGCTTGGGCTGAGAATGAAGCAAATGAATTGATAAAGAAGTATGATAGAAATAGTGATGGTGTTCTCACTAAAGAAGAGATACAATCTTTCCTCCACGACGCTGGTTGTTCAACTCAACTCGACAATCTAAAGTTTGTGTCATCTAAGGTGATTTCAAAAACTAACGACAATCTTGTGCACAAGCTTAGCTCAAACACTCATATGAATTCGAAAAAACCATTGCGAGACAAAACTCAAAATTTATCGACAGAAGTTCTGGATAGAAAGCAATCTAATGTTCCTCTGAGTCGAGAGCAGATAAAGGAGATTTTTAAATATCATGATAGCGACAAAGATGGTTTTCTCAACATACGAGAATTAACAAAAGCTTTCGCCTCTCTGGGCTCAATTATTCCATTCCACAAAGCTCGCTACGCTATGATTTATGCTGATGCTGATAAAGATGGTCTTATCAGTGAAGCCGAACTTGACAAACTCATTGACTATGCTAAAAAAATCATCCAGAAGAAATGAGCTTACAAGAACATTCTCGTACATTTTATGTAGGGAAGATAATTATGTAACCAGAAATAAAATATTTAAAAATAAAATAAAACAGACGTTATGTTAGAATTAAAAATCTTATCTTTTATTATTAAGTGTTAATATTAATTTCTTATGGTTCATTTCTCTTTGTTAATAATCAACAAACACATGAATATTATTTTCTTATGGTTCATTTCTCTTTGTTAATAATCAACAAACACATGAATATTATTAACTATTTGGCCGTAATCTAAACATAATCTCAAATTGAACTAAAAGTTGAAATTTGATATAATTAACTCCTCCCAATTCATTCAAATTTTATTTTTGATTATATATGGAATCTCACTCACTATATATGATTTAGAAAGGTTTCCAAACTTATAATACTTCGGGTATTATTCTGTAAATTGGATTTAAAAACTTACTATTGATAGCTTCCTTCCAATAAGGTGCATCAGACTAGTTCACCATATAAGTTGGACAAACTCATTATTATAGCTCATTATTCAAACTCACTAATTGACAACTTCTTGAAAATTTTGAGGCTCACTTTCTATTATACAAGTTATGAAATCCAGTCCAAAAGAAGTAACATTCTTAGTTCTTTTGCTTCCCTCGTAATTTCTTTTGGCGCTATTAATTTTGGCATAGAAATAGGTGTATATGCCCAGAAACACCAATATTCTTTTACAAATTACATTAGTAATTTATATGCATATATAGATATGTGTGAATTTGTGGATATATAGATGGGAAATCTAGCAATTATTTTGTTATTATTTTTGTTTACTTATAATGGATAAAAATTATTTTGCATTTCGTTAGATTTTGTTTATTTGATGTCCTTAATTATAGTGGTTGGATAGTTAATAAATAATTTATTTTTTTAAAAAAAGGCAAAATCTTATAAGAAGGTAATTTTGTTACAAGAAAACTAACAAATTCTCTTTAGAGTGTGTTTGGAATACTTTTTAAGTGTTTAATTGTTTTAAAAAAATTATTTTAGAAATAATTAAGTGTTTGGCAACCATTCAAAATAGATTTTGAAACACATTAGTGCTTTTATTTTAAACCGTTTTTATCAAAAGTATTTAAATAAAAAAGAATTTTTGAAAAGAATTTTTTTTAAAATTAATCCAAACGAGCCCTTAAATTTTTTTCCAATGAAACTTTGTTTTAGATATTTTTGAAATATAATTATTTAAAATTAGCTATTTTGTGCTTCACCCTAATAACTTCCTACGTTGTGGGTCCTATAACTATGGAGTGACTCATTTCAAATTAATTAATAGAGAACATTTAAAATTAAGTGATTGTTTTTAAATTTTTGGCTACATTTAAAATTAAGTGATTATTTTTAAATTTCTGGCTATATATATATATATATATATATATATATATATGGAGAATTCTTAAAAATAAAAAAAATAAGAAAAACTATTTACACAAAATAGTAAAATTTTTAGATAGTTATGATAGCAAAATTTTTAGATAGTTGTAATAGATATTGATAGAAGTCTATCAATATTTATTCGTATTTTTTCTTTTTTTGTTATTTTCTATAAATAATTTGACATTTTTTTTATCTGGAAAAATTTTCTAATATATATATATATATAAAGTTTACTAATGGAATTCTTCCAGAGAGAGAGGTATAGTGGAGAAACATGTTTTTTATTTTTCTTGCAAATTTAGTGGAGAAATTTCTATAGCATTATCCCTTTTGTACTGATGTTTGGTTAAAATGGAACATCTTTTTACAAAGGTACGGTATGAGATTCGAATTTTATAGATAATAAGAATGACAATAATTTTACTGTTAGAGTAATGTTCAATTCACCTGAAAATAAACAAGGTGGTCAAAGTCAAACCAAGTTCCTTTTCGAGCTACTATAAAATACTTGAACCATCTTAAGATTTGTTTCATCTTCAAACTTTCCAACCAAACAAAAATATAGTATTAGTTTAAGAATGTCGGTCTCCCAAATTCAAGCTTGGGCTGAGAATGAAGCAAATGAATTGATAAAGAAGTATGATAGAAATGGTGATGGTGTTCTCACTAAAGAAGAGCTACCATCTTTTCTCCATGACACTCGTTGTTCAACTCAACTCAACAATTCAAGGTTTACATCATCTAAGACGTTCCCAAAAACTAACGATAATCTTGCATACAAGCTTACCTCAAACACTCCTATGAACCCAAAAGACCAGTTGCGAGACAAAGCTCAAAATTCCTCAGCAAAATTTCTTGATAGAAAGCAATCTAATGTGTCTTTGAGTCGAGAGCAGATAAAAGAGATTTTTAAATATCATGACAGTGACAAAGATGGTTTTCTCAACATACGGGAGTTAACAAAAGCTTTCGGTTCTCTAGGCTCAGGTGTCCCATTCAACAAAGCTCGCTATGGTATGATTTATGCTGATGCTGATAAAGATGGTCTTATTAGTGAGGTCGAACTTGACAAACTCATCGATTATGCCATTAAAATCATCAAGAAGAAATGA

mRNA sequence

ATGTCGGCCTCCCAACTTCAAGCTTGGGCTGAGAATGAAGCAAATGAATTGATAAAGAAGTATGATAGAAATAGTGATGGTGTTCTCACTAAAGAAGAGATACAATCTTTCCTCCACGACGCTGGTTGTTCAACTCAACTCGACAATCTAAAGTTTGTGTCATCTAAGAAAGGTTTCCAAACTTATAATACTTCGGGTATTATTCTTATTAGTTTAAGAATGTCGGTCTCCCAAATTCAAGCTTGGGCTGAGAATGAAGCAAATGAATTGATAAAGAAGTATGATAGAAATGGTGATGGTGTTCTCACTAAAGAAGAGCTACCATCTTTTCTCCATGACACTCGTTGTTCAACTCAACTCAACAATTCAAGGTTTACATCATCTAAGACGTTCCCAAAAACTAACGATAATCTTGCATACAAGCTTACCTCAAACACTCCTATGAACCCAAAAGACCAGTTGCGAGACAAAGCTCAAAATTCCTCAGCAAAATTTCTTGATAGAAAGCAATCTAATGTGTCTTTGAGTCGAGAGCAGATAAAAGAGATTTTTAAATATCATGACAGTGACAAAGATGGTTTTCTCAACATACGGGAGTTAACAAAAGCTTTCGGTTCTCTAGGCTCAGGTGTCCCATTCAACAAAGCTCGCTATGGTATGATTTATGCTGATGCTGATAAAGATGGTCTTATTAGTGAGGTCGAACTTGACAAACTCATCGATTATGCCATTAAAATCATCAAGAAGAAATGA

Coding sequence (CDS)

ATGTCGGCCTCCCAACTTCAAGCTTGGGCTGAGAATGAAGCAAATGAATTGATAAAGAAGTATGATAGAAATAGTGATGGTGTTCTCACTAAAGAAGAGATACAATCTTTCCTCCACGACGCTGGTTGTTCAACTCAACTCGACAATCTAAAGTTTGTGTCATCTAAGAAAGGTTTCCAAACTTATAATACTTCGGGTATTATTCTTATTAGTTTAAGAATGTCGGTCTCCCAAATTCAAGCTTGGGCTGAGAATGAAGCAAATGAATTGATAAAGAAGTATGATAGAAATGGTGATGGTGTTCTCACTAAAGAAGAGCTACCATCTTTTCTCCATGACACTCGTTGTTCAACTCAACTCAACAATTCAAGGTTTACATCATCTAAGACGTTCCCAAAAACTAACGATAATCTTGCATACAAGCTTACCTCAAACACTCCTATGAACCCAAAAGACCAGTTGCGAGACAAAGCTCAAAATTCCTCAGCAAAATTTCTTGATAGAAAGCAATCTAATGTGTCTTTGAGTCGAGAGCAGATAAAAGAGATTTTTAAATATCATGACAGTGACAAAGATGGTTTTCTCAACATACGGGAGTTAACAAAAGCTTTCGGTTCTCTAGGCTCAGGTGTCCCATTCAACAAAGCTCGCTATGGTATGATTTATGCTGATGCTGATAAAGATGGTCTTATTAGTGAGGTCGAACTTGACAAACTCATCGATTATGCCATTAAAATCATCAAGAAGAAATGA

Protein sequence

MSASQLQAWAENEANELIKKYDRNSDGVLTKEEIQSFLHDAGCSTQLDNLKFVSSKKGFQTYNTSGIILISLRMSVSQIQAWAENEANELIKKYDRNGDGVLTKEELPSFLHDTRCSTQLNNSRFTSSKTFPKTNDNLAYKLTSNTPMNPKDQLRDKAQNSSAKFLDRKQSNVSLSREQIKEIFKYHDSDKDGFLNIRELTKAFGSLGSGVPFNKARYGMIYADADKDGLISEVELDKLIDYAIKIIKKK

Homology

[Close]

BLAST of Lsi08G014600 vs. ExPASy Swiss-Prot
Match: Q9LQN4 (Probable calcium-binding protein CML17 OS=Arabidopsis thaliana OX=3702 GN=CML17 PE=2 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 7.4e-05
Identity = 44/159 (27.67%), Postives = 69/159 (43.40%), Query Frame = 0


Query: 85  NEANELIKKYDRNGDGVLTKEELPSFLHDTRCSTQLNNSRFTSSKTFPKTNDNLAYKLTS 144
           NE  E+ + +DRN DG LT+ EL S L            R    K  P   + L  K  +
Sbjct: 15  NELREIFRSFDRNKDGSLTQLELGSLL------------RALGVKPSPDQFETLIDK--A 74

Query: 145 NTPMNPKDQLRDKAQNSSAKFLDRKQSNVSLSREQIKEIFKYHDSDKDGFLNIRELTKAF 204
           +T  N   +  +     S + L   +     + EQ+  +F+  D+D +GF+   EL  + 
Sbjct: 75  DTKSNGLVEFPEFVALVSPELLSPAKRTTPYTEEQLLRLFRIFDTDGNGFITAAELAHSM 134

Query: 205 GSLGSGVPFNKARYGMIYADADKDGLISEVELDKLIDYA 244
             LG  +   +    +  AD+D DG I+  E  K I+ A
Sbjct: 135 AKLGHALTVAELTGMIKEADSDGDGRINFQEFAKAINSA 159

[Close]

BLAST of Lsi08G014600 vs. ExPASy Swiss-Prot
Match: O22845 (Calmodulin-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=CML5 PE=2 SV=2)

HSP 1 Score: 45.8 bits (107), Expect = 8.2e-04
Identity = 38/153 (24.84%), Postives = 67/153 (43.79%), Query Frame = 0


Query: 85  NEANELIKKYDRNGDGVLTKEELPSFLHDTRCSTQLNNSRFTSSKTFPKTNDNLAYKLTS 144
           +E   + + +D+NGDG +TKEEL   L         N   +   K   +    + +K+ +
Sbjct: 64  SELKRVFQMFDKNGDGRITKEELNDSLE--------NLGIYIPDKDLTQ----MIHKIDA 123

Query: 145 NTPMNPKDQLRDKAQNSSAKFLDRKQSNVSLSREQIKEIFKYHDSDKDGFLNIRELTKAF 204
           N          D+ ++  +  +D   ++     E +K+ F   D D DGF+ + EL    
Sbjct: 124 N---GDGCVDIDEFESLYSSIVDEHHNDGETEEEDMKDAFNVFDQDGDGFITVEELKSVM 183

Query: 205 GSLG--SGVPFNKARYGMIYADADKDGLISEVE 236
            SLG   G   +  +  ++  DAD DG ++  E
Sbjct: 184 ASLGLKQGKTLDGCKKMIMQVDADGDGRVNYKE 201

[Close]

BLAST of Lsi08G014600 vs. ExPASy TrEMBL
Match: A0A0A0LRC8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G359950 PE=4 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 2.3e-62
Identity = 135/177 (76.27%), Postives = 146/177 (82.49%), Query Frame = 0


Query: 74  MSVSQIQAWAENEANELIKKYDRNGDGVLTKEELPSFLHDTRCSTQLNNSRFTSSKTFPK 133
           MSVS +QAWA+NEANELIKKYDRNGDGVLTKEEL SFL D R STQLNN R  SS T PK
Sbjct: 1   MSVSHLQAWAKNEANELIKKYDRNGDGVLTKEELQSFLRDARGSTQLNNIRNVSSSTVPK 60

Query: 134 TNDNLAYKLTSNTPMNPKDQLRDKAQNSSAKFLDRKQSNVSLSREQIKEIFKYHDSDKDG 193
           TN+NLA K++SNT + PK QL DKAQ SS      KQSNV LSREQIKEIFKYHDS+KDG
Sbjct: 61  TNENLAQKVSSNTCITPKKQLLDKAQGSS------KQSNVPLSREQIKEIFKYHDSNKDG 120

Query: 194 FLNIRELTKAFGSLGSGVPFNKARYGMIYADADKDGLISEVELDKLIDYAIKIIKKK 251
           FL+IRELTKAF S+GS VPF KARY M YAD D+DGL+SE ELDKLIDYA KIIKKK
Sbjct: 121 FLSIRELTKAFSSMGSIVPFCKARYAMAYADVDEDGLVSEAELDKLIDYAHKIIKKK 171

[Close]

BLAST of Lsi08G014600 vs. ExPASy TrEMBL
Match: A0A5A7V4R9 (Putative calcium-binding protein CML11 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00180 PE=4 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 2.3e-57
Identity = 129/177 (72.88%), Postives = 142/177 (80.23%), Query Frame = 0


Query: 74  MSVSQIQAWAENEANELIKKYDRNGDGVLTKEELPSFLHDTRCSTQLNNSRFTSSKTFPK 133
           MSVS +QAWA+ EA+ELIKKYDRNGDGVLTKEEL SFLHD R    L N RF SS T PK
Sbjct: 1   MSVSHLQAWAKIEASELIKKYDRNGDGVLTKEELQSFLHDAR----LKNIRFVSSSTIPK 60

Query: 134 TNDNLAYKLTSNTPMNPKDQLRDKAQNSSAKFLDRKQSNVSLSREQIKEIFKYHDSDKDG 193
           TN+NL  K++SNT + PK QL DKAQ+S       +QSNV LSREQIKEIFKYHDS+KDG
Sbjct: 61  TNENLDQKVSSNTRIKPKKQLLDKAQDS------LRQSNVPLSREQIKEIFKYHDSNKDG 120

Query: 194 FLNIRELTKAFGSLGSGVPFNKARYGMIYADADKDGLISEVELDKLIDYAIKIIKKK 251
           FL+IRELTKAFGSLGS VPF KAR+ M YAD D+DGLISE EL KLIDYA KIIKKK
Sbjct: 121 FLSIRELTKAFGSLGSIVPFAKARHAMAYADVDEDGLISEAELHKLIDYAEKIIKKK 167

[Close]

BLAST of Lsi08G014600 vs. ExPASy TrEMBL
Match: A0A1S3BDW2 (probable calcium-binding protein CML11 OS=Cucumis melo OX=3656 GN=LOC103488544 PE=4 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 2.3e-57
Identity = 129/177 (72.88%), Postives = 142/177 (80.23%), Query Frame = 0


Query: 74  MSVSQIQAWAENEANELIKKYDRNGDGVLTKEELPSFLHDTRCSTQLNNSRFTSSKTFPK 133
           MSVS +QAWA+ EA+ELIKKYDRNGDGVLTKEEL SFLHD R    L N RF SS T PK
Sbjct: 1   MSVSHLQAWAKIEASELIKKYDRNGDGVLTKEELQSFLHDAR----LKNIRFVSSSTIPK 60

Query: 134 TNDNLAYKLTSNTPMNPKDQLRDKAQNSSAKFLDRKQSNVSLSREQIKEIFKYHDSDKDG 193
           TN+NL  K++SNT + PK QL DKAQ+S       +QSNV LSREQIKEIFKYHDS+KDG
Sbjct: 61  TNENLDQKVSSNTRIKPKKQLLDKAQDS------LRQSNVPLSREQIKEIFKYHDSNKDG 120

Query: 194 FLNIRELTKAFGSLGSGVPFNKARYGMIYADADKDGLISEVELDKLIDYAIKIIKKK 251
           FL+IRELTKAFGSLGS VPF KAR+ M YAD D+DGLISE EL KLIDYA KIIKKK
Sbjct: 121 FLSIRELTKAFGSLGSIVPFAKARHAMAYADVDEDGLISEAELHKLIDYAEKIIKKK 167

[Close]

BLAST of Lsi08G014600 vs. ExPASy TrEMBL
Match: A0A6J1KM01 (probable calcium-binding protein CML11 OS=Cucurbita maxima OX=3661 GN=LOC111496473 PE=4 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 4.3e-56
Identity = 124/177 (70.06%), Postives = 139/177 (78.53%), Query Frame = 0


Query: 74  MSVSQIQAWAENEANELIKKYDRNGDGVLTKEELPSFLHDTRCSTQLNNSRFTSSKTFPK 133
           MSVS +Q WAENEANEL+KKYD+NGDG+LTKEEL  FL D R STQLN   F S    PK
Sbjct: 1   MSVSILQTWAENEANELMKKYDKNGDGILTKEELQVFLRDVRGSTQLNYQGFLSK--IPK 60

Query: 134 TNDNLAYKLTSNTPMNPKDQLRDKAQNSSAKFLDRKQSNVSLSREQIKEIFKYHDSDKDG 193
           TND  A+KL+SNT  NPK Q++DK   SS K L  K SNVSLSREQIK+IFKY+DSDKDG
Sbjct: 61  TNDKGAHKLSSNTHTNPKIQVQDKVTKSSTKVLSEKVSNVSLSREQIKKIFKYYDSDKDG 120

Query: 194 FLNIRELTKAFGSLGSGVPFNKARYGMIYADADKDGLISEVELDKLIDYAIKIIKKK 251
           FLNIRE+TKAF  LGS  PF KA+Y M +ADA+ DGLISE EL+KLIDYA KIIKKK
Sbjct: 121 FLNIREVTKAFSFLGSINPFYKAQYAMTFADANNDGLISETELEKLIDYACKIIKKK 175

[Close]

BLAST of Lsi08G014600 vs. ExPASy TrEMBL
Match: A0A6J1GII5 (probable calcium-binding protein CML11 OS=Cucurbita moschata OX=3662 GN=LOC111454483 PE=4 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 1.6e-55
Identity = 124/177 (70.06%), Postives = 138/177 (77.97%), Query Frame = 0


Query: 74  MSVSQIQAWAENEANELIKKYDRNGDGVLTKEELPSFLHDTRCSTQLNNSRFTSSKTFPK 133
           MSVS +QAWAEN+ANELIKKYD+NGDGVLTKEEL  FL D R STQLNN  F S    P+
Sbjct: 1   MSVSILQAWAENKANELIKKYDKNGDGVLTKEELQVFLRDVRGSTQLNNQGFLSK--IPR 60

Query: 134 TNDNLAYKLTSNTPMNPKDQLRDKAQNSSAKFLDRKQSNVSLSREQIKEIFKYHDSDKDG 193
           TND  A+KL SN   NPK Q++ KA  SS K L  K SNV LSREQIK+IFKY+DSDKDG
Sbjct: 61  TNDKGAHKLNSNPRTNPKIQVQSKATKSSTKVLSEKVSNVPLSREQIKKIFKYYDSDKDG 120

Query: 194 FLNIRELTKAFGSLGSGVPFNKARYGMIYADADKDGLISEVELDKLIDYAIKIIKKK 251
           FLNIRE+TKAF  LGS  PF KA+Y M +ADA+ DGLISE EL+KLIDYA KIIKKK
Sbjct: 121 FLNIREVTKAFSFLGSINPFYKAQYAMTFADANNDGLISEAELEKLIDYAYKIIKKK 175

[Close]

BLAST of Lsi08G014600 vs. NCBI nr
Match: XP_038886759.1 (probable calcium-binding protein CML11 [Benincasa hispida])

HSP 1 Score: 269.6 bits (688), Expect = 2.7e-68
Identity = 141/177 (79.66%), Postives = 151/177 (85.31%), Query Frame = 0


Query: 74  MSVSQIQAWAENEANELIKKYDRNGDGVLTKEELPSFLHDTRCSTQLNNSRFTSSKTFPK 133
           MSVSQ+QAWAENEANELIKKYDRNGDGVL KEEL SFLHDT  STQLNN+R   S+  PK
Sbjct: 1   MSVSQLQAWAENEANELIKKYDRNGDGVLAKEELQSFLHDTCSSTQLNNTRCVLSRMIPK 60

Query: 134 TNDNLAYKLTSNTPMNPKDQLRDKAQNSSAKFLDRKQSNVSLSREQIKEIFKYHDSDKDG 193
           TN+NL +KL+SNT MN K QLRDK QNSS K LDRKQSN+  SREQIKEIFKYHDS+KDG
Sbjct: 61  TNNNLVHKLSSNTHMNQKKQLRDKIQNSSTKALDRKQSNIPPSREQIKEIFKYHDSNKDG 120

Query: 194 FLNIRELTKAFGSLGSGVPFNKARYGMIYADADKDGLISEVELDKLIDYAIKIIKKK 251
           FLNIRELTK FGSLGS +PF KA Y M YADADKDGLISEVELD+LIDY  KIIKKK
Sbjct: 121 FLNIRELTKTFGSLGSVIPFYKAHYAMAYADADKDGLISEVELDELIDYTNKIIKKK 177

[Close]

BLAST of Lsi08G014600 vs. NCBI nr
Match: XP_004152801.1 (probable calcium-binding protein CML11 [Cucumis sativus] >KGN62526.1 hypothetical protein Csa_021903 [Cucumis sativus])

HSP 1 Score: 248.8 bits (634), Expect = 4.9e-62
Identity = 135/177 (76.27%), Postives = 146/177 (82.49%), Query Frame = 0


Query: 74  MSVSQIQAWAENEANELIKKYDRNGDGVLTKEELPSFLHDTRCSTQLNNSRFTSSKTFPK 133
           MSVS +QAWA+NEANELIKKYDRNGDGVLTKEEL SFL D R STQLNN R  SS T PK
Sbjct: 1   MSVSHLQAWAKNEANELIKKYDRNGDGVLTKEELQSFLRDARGSTQLNNIRNVSSSTVPK 60

Query: 134 TNDNLAYKLTSNTPMNPKDQLRDKAQNSSAKFLDRKQSNVSLSREQIKEIFKYHDSDKDG 193
           TN+NLA K++SNT + PK QL DKAQ SS      KQSNV LSREQIKEIFKYHDS+KDG
Sbjct: 61  TNENLAQKVSSNTCITPKKQLLDKAQGSS------KQSNVPLSREQIKEIFKYHDSNKDG 120

Query: 194 FLNIRELTKAFGSLGSGVPFNKARYGMIYADADKDGLISEVELDKLIDYAIKIIKKK 251
           FL+IRELTKAF S+GS VPF KARY M YAD D+DGL+SE ELDKLIDYA KIIKKK
Sbjct: 121 FLSIRELTKAFSSMGSIVPFCKARYAMAYADVDEDGLVSEAELDKLIDYAHKIIKKK 171

[Close]

BLAST of Lsi08G014600 vs. NCBI nr
Match: XP_008445564.1 (PREDICTED: probable calcium-binding protein CML11 [Cucumis melo] >KAA0060885.1 putative calcium-binding protein CML11 [Cucumis melo var. makuwa])

HSP 1 Score: 232.3 bits (591), Expect = 4.7e-57
Identity = 129/177 (72.88%), Postives = 142/177 (80.23%), Query Frame = 0


Query: 74  MSVSQIQAWAENEANELIKKYDRNGDGVLTKEELPSFLHDTRCSTQLNNSRFTSSKTFPK 133
           MSVS +QAWA+ EA+ELIKKYDRNGDGVLTKEEL SFLHD R    L N RF SS T PK
Sbjct: 1   MSVSHLQAWAKIEASELIKKYDRNGDGVLTKEELQSFLHDAR----LKNIRFVSSSTIPK 60

Query: 134 TNDNLAYKLTSNTPMNPKDQLRDKAQNSSAKFLDRKQSNVSLSREQIKEIFKYHDSDKDG 193
           TN+NL  K++SNT + PK QL DKAQ+S       +QSNV LSREQIKEIFKYHDS+KDG
Sbjct: 61  TNENLDQKVSSNTRIKPKKQLLDKAQDS------LRQSNVPLSREQIKEIFKYHDSNKDG 120

Query: 194 FLNIRELTKAFGSLGSGVPFNKARYGMIYADADKDGLISEVELDKLIDYAIKIIKKK 251
           FL+IRELTKAFGSLGS VPF KAR+ M YAD D+DGLISE EL KLIDYA KIIKKK
Sbjct: 121 FLSIRELTKAFGSLGSIVPFAKARHAMAYADVDEDGLISEAELHKLIDYAEKIIKKK 167

[Close]

BLAST of Lsi08G014600 vs. NCBI nr
Match: XP_023002691.1 (probable calcium-binding protein CML11 [Cucurbita maxima])

HSP 1 Score: 228.0 bits (580), Expect = 8.9e-56
Identity = 124/177 (70.06%), Postives = 139/177 (78.53%), Query Frame = 0


Query: 74  MSVSQIQAWAENEANELIKKYDRNGDGVLTKEELPSFLHDTRCSTQLNNSRFTSSKTFPK 133
           MSVS +Q WAENEANEL+KKYD+NGDG+LTKEEL  FL D R STQLN   F S    PK
Sbjct: 1   MSVSILQTWAENEANELMKKYDKNGDGILTKEELQVFLRDVRGSTQLNYQGFLSK--IPK 60

Query: 134 TNDNLAYKLTSNTPMNPKDQLRDKAQNSSAKFLDRKQSNVSLSREQIKEIFKYHDSDKDG 193
           TND  A+KL+SNT  NPK Q++DK   SS K L  K SNVSLSREQIK+IFKY+DSDKDG
Sbjct: 61  TNDKGAHKLSSNTHTNPKIQVQDKVTKSSTKVLSEKVSNVSLSREQIKKIFKYYDSDKDG 120

Query: 194 FLNIRELTKAFGSLGSGVPFNKARYGMIYADADKDGLISEVELDKLIDYAIKIIKKK 251
           FLNIRE+TKAF  LGS  PF KA+Y M +ADA+ DGLISE EL+KLIDYA KIIKKK
Sbjct: 121 FLNIREVTKAFSFLGSINPFYKAQYAMTFADANNDGLISETELEKLIDYACKIIKKK 175

[Close]

BLAST of Lsi08G014600 vs. NCBI nr
Match: XP_022951742.1 (probable calcium-binding protein CML11 [Cucurbita moschata])

HSP 1 Score: 226.1 bits (575), Expect = 3.4e-55
Identity = 124/177 (70.06%), Postives = 138/177 (77.97%), Query Frame = 0


Query: 74  MSVSQIQAWAENEANELIKKYDRNGDGVLTKEELPSFLHDTRCSTQLNNSRFTSSKTFPK 133
           MSVS +QAWAEN+ANELIKKYD+NGDGVLTKEEL  FL D R STQLNN  F S    P+
Sbjct: 1   MSVSILQAWAENKANELIKKYDKNGDGVLTKEELQVFLRDVRGSTQLNNQGFLSK--IPR 60

Query: 134 TNDNLAYKLTSNTPMNPKDQLRDKAQNSSAKFLDRKQSNVSLSREQIKEIFKYHDSDKDG 193
           TND  A+KL SN   NPK Q++ KA  SS K L  K SNV LSREQIK+IFKY+DSDKDG
Sbjct: 61  TNDKGAHKLNSNPRTNPKIQVQSKATKSSTKVLSEKVSNVPLSREQIKKIFKYYDSDKDG 120

Query: 194 FLNIRELTKAFGSLGSGVPFNKARYGMIYADADKDGLISEVELDKLIDYAIKIIKKK 251
           FLNIRE+TKAF  LGS  PF KA+Y M +ADA+ DGLISE EL+KLIDYA KIIKKK
Sbjct: 121 FLNIREVTKAFSFLGSINPFYKAQYAMTFADANNDGLISEAELEKLIDYAYKIIKKK 175

[Close]

BLAST of Lsi08G014600 vs. TAIR 10
Match: AT1G32250.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 49.3 bits (116), Expect = 5.3e-06
Identity = 44/159 (27.67%), Postives = 69/159 (43.40%), Query Frame = 0


Query: 85  NEANELIKKYDRNGDGVLTKEELPSFLHDTRCSTQLNNSRFTSSKTFPKTNDNLAYKLTS 144
           NE  E+ + +DRN DG LT+ EL S L            R    K  P   + L  K  +
Sbjct: 15  NELREIFRSFDRNKDGSLTQLELGSLL------------RALGVKPSPDQFETLIDK--A 74

Query: 145 NTPMNPKDQLRDKAQNSSAKFLDRKQSNVSLSREQIKEIFKYHDSDKDGFLNIRELTKAF 204
           +T  N   +  +     S + L   +     + EQ+  +F+  D+D +GF+   EL  + 
Sbjct: 75  DTKSNGLVEFPEFVALVSPELLSPAKRTTPYTEEQLLRLFRIFDTDGNGFITAAELAHSM 134

Query: 205 GSLGSGVPFNKARYGMIYADADKDGLISEVELDKLIDYA 244
             LG  +   +    +  AD+D DG I+  E  K I+ A
Sbjct: 135 AKLGHALTVAELTGMIKEADSDGDGRINFQEFAKAINSA 159

[Close]

BLAST of Lsi08G014600 vs. TAIR 10
Match: AT2G43290.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 45.8 bits (107), Expect = 5.8e-05
Identity = 38/153 (24.84%), Postives = 67/153 (43.79%), Query Frame = 0


Query: 85  NEANELIKKYDRNGDGVLTKEELPSFLHDTRCSTQLNNSRFTSSKTFPKTNDNLAYKLTS 144
           +E   + + +D+NGDG +TKEEL   L         N   +   K   +    + +K+ +
Sbjct: 64  SELKRVFQMFDKNGDGRITKEELNDSLE--------NLGIYIPDKDLTQ----MIHKIDA 123

Query: 145 NTPMNPKDQLRDKAQNSSAKFLDRKQSNVSLSREQIKEIFKYHDSDKDGFLNIRELTKAF 204
           N          D+ ++  +  +D   ++     E +K+ F   D D DGF+ + EL    
Sbjct: 124 N---GDGCVDIDEFESLYSSIVDEHHNDGETEEEDMKDAFNVFDQDGDGFITVEELKSVM 183

Query: 205 GSLG--SGVPFNKARYGMIYADADKDGLISEVE 236
            SLG   G   +  +  ++  DAD DG ++  E
Sbjct: 184 ASLGLKQGKTLDGCKKMIMQVDADGDGRVNYKE 201

[Close]

BLAST of Lsi08G014600 vs. TAIR 10
Match: AT4G12860.1 (EF hand calcium-binding protein family )

HSP 1 Score: 42.4 bits (98), Expect = 6.4e-04
Identity = 28/102 (27.45%), Postives = 48/102 (47.06%), Query Frame = 0


Query: 13  EANELIKKYDRNSDGVLTKEEIQSFLHDAGCSTQLDNLKFVSSKKGFQTYNTSGIILISL 72
           E + + + +D+N DG + K E++ F    G     + +  + +K      N  G + I  
Sbjct: 5   ELSRVFQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAK---MDVNGDGAMDIDE 64

Query: 73  RMSVSQ---IQAWAENEANELIKKYDRNGDGVLTKEELPSFL 112
             S+ Q    +   E +  E  + +D+NGDG +T EEL S L
Sbjct: 65  FGSLYQEMVEEKEEEEDMREAFRVFDQNGDGFITDEELRSVL 103

The following BLAST results are available for this feature:

BLAST of Lsi08G014600 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LQN4	7.4e-05	27.67	Probable calcium-binding protein CML17 OS=Arabidopsis thaliana OX=3702 GN=CML17 ...	[more]
O22845	8.2e-04	24.84	Calmodulin-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=CML5 PE=2 SV=2	[more]

BLAST of Lsi08G014600 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LRC8	2.3e-62	76.27	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G359950 PE=4 SV=1	[more]
A0A5A7V4R9	2.3e-57	72.88	Putative calcium-binding protein CML11 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3BDW2	2.3e-57	72.88	probable calcium-binding protein CML11 OS=Cucumis melo OX=3656 GN=LOC103488544 P...	[more]
A0A6J1KM01	4.3e-56	70.06	probable calcium-binding protein CML11 OS=Cucurbita maxima OX=3661 GN=LOC1114964...	[more]
A0A6J1GII5	1.6e-55	70.06	probable calcium-binding protein CML11 OS=Cucurbita moschata OX=3662 GN=LOC11145...	[more]

BLAST of Lsi08G014600 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038886759.1	2.7e-68	79.66	probable calcium-binding protein CML11 [Benincasa hispida]	[more]
XP_004152801.1	4.9e-62	76.27	probable calcium-binding protein CML11 [Cucumis sativus] >KGN62526.1 hypothetica...	[more]
XP_008445564.1	4.7e-57	72.88	PREDICTED: probable calcium-binding protein CML11 [Cucumis melo] >KAA0060885.1 p...	[more]
XP_023002691.1	8.9e-56	70.06	probable calcium-binding protein CML11 [Cucurbita maxima]	[more]
XP_022951742.1	3.4e-55	70.06	probable calcium-binding protein CML11 [Cucurbita moschata]	[more]

BLAST of Lsi08G014600 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G32250.1	5.3e-06	27.67	Calcium-binding EF-hand family protein	[more]
AT2G43290.1	5.8e-05	24.84	Calcium-binding EF-hand family protein	[more]
AT4G12860.1	6.4e-04	27.45	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 86..114 e-value: 0.0061 score: 25.8 coord: 13..41 e-value: 0.02 score: 24.1 coord: 179..207 e-value: 0.068 score: 22.3
IPR002048	EF-hand domain	PFAM	PF13202	EF-hand_5	coord: 16..37 e-value: 0.0044 score: 16.5 coord: 89..107 e-value: 7.1E-4 score: 19.0
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 177..236 e-value: 4.7E-8 score: 33.4
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 82..117 score: 11.472807
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 175..210 score: 13.174425
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 9..44 score: 12.532831
None	No IPR available	GENE3D	1.10.238.10		coord: 76..109 e-value: 1.6E-5 score: 26.6
None	No IPR available	GENE3D	1.10.238.10		coord: 9..46 e-value: 3.0E-5 score: 25.5
None	No IPR available	GENE3D	1.10.238.10		coord: 119..243 e-value: 2.9E-11 score: 45.6
None	No IPR available	PANTHER	PTHR10891:SF892	CALCIUM-BINDING PROTEIN CML28-RELATED	coord: 85..122
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 85..122 coord: 12..49 coord: 178..236
None	No IPR available	PANTHER	PTHR10891:SF892	CALCIUM-BINDING PROTEIN CML28-RELATED	coord: 12..49 coord: 178..236
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 22..34
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 188..200
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 95..107
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 224..236
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 4..236

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi08G014600.1	Lsi08G014600.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding