Lsi08G014410 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi08G014410
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionProtein kinase domain-containing protein
Locationchr08: 22600747 .. 22601121 (+)
RNA-Seq ExpressionLsi08G014410
SyntenyLsi08G014410
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCACTCCCAATTGAACAACAATCTCCGCCGGCGTCGGGAATGCAGCAAAATTATGGCGGCCATCCCGGCAGCGGCTCCGTCGGTCCGGTTATCGGAGTCCTCGTCGTCATCATTGTGTTCGCCGTGGTCGCCGTCGTGGTCGGGCGGCTGTGCTCGGGAAGGAGCATAATGGGCTACGGTCATTTCGACTTGGAGAGCTGGGCGGAGACAAAATGCTCCTCTTGCATCGACGGAAGGATCAGCCCACCACCGCCGAGGGTTAATGTCTCCGCCGCCGCCGACTCTCTTCCGGCCGGGACAATGGCGGTCCGAACCCAGCAAGAAACTAAGCAAGAAGAACCAGCTTCTCAAAATCCACCTGCCAACGCTTGA

mRNA sequence

ATGTCACTCCCAATTGAACAACAATCTCCGCCGGCGTCGGGAATGCAGCAAAATTATGGCGGCCATCCCGGCAGCGGCTCCGTCGGTCCGGTTATCGGAGTCCTCGTCGTCATCATTGTGTTCGCCGTGGTCGCCGTCGTGGTCGGGCGGCTGTGCTCGGGAAGGAGCATAATGGGCTACGGTCATTTCGACTTGGAGAGCTGGGCGGAGACAAAATGCTCCTCTTGCATCGACGGAAGGATCAGCCCACCACCGCCGAGGGTTAATGTCTCCGCCGCCGCCGACTCTCTTCCGGCCGGGACAATGGCGGTCCGAACCCAGCAAGAAACTAAGCAAGAAGAACCAGCTTCTCAAAATCCACCTGCCAACGCTTGA

Coding sequence (CDS)

ATGTCACTCCCAATTGAACAACAATCTCCGCCGGCGTCGGGAATGCAGCAAAATTATGGCGGCCATCCCGGCAGCGGCTCCGTCGGTCCGGTTATCGGAGTCCTCGTCGTCATCATTGTGTTCGCCGTGGTCGCCGTCGTGGTCGGGCGGCTGTGCTCGGGAAGGAGCATAATGGGCTACGGTCATTTCGACTTGGAGAGCTGGGCGGAGACAAAATGCTCCTCTTGCATCGACGGAAGGATCAGCCCACCACCGCCGAGGGTTAATGTCTCCGCCGCCGCCGACTCTCTTCCGGCCGGGACAATGGCGGTCCGAACCCAGCAAGAAACTAAGCAAGAAGAACCAGCTTCTCAAAATCCACCTGCCAACGCTTGA

Protein sequence

MSLPIEQQSPPASGMQQNYGGHPGSGSVGPVIGVLVVIIVFAVVAVVVGRLCSGRSIMGYGHFDLESWAETKCSSCIDGRISPPPPRVNVSAAADSLPAGTMAVRTQQETKQEEPASQNPPANA
Homology
BLAST of Lsi08G014410 vs. ExPASy TrEMBL
Match: A0A0A0LP67 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G360640 PE=4 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 7.1e-52
Identity = 114/126 (90.48%), Postives = 117/126 (92.86%), Query Frame = 0

Query: 1   MSLPIEQQSPPASGMQQNYGGHPGSGSVGPVIGVLVVIIVFAVVAVVVGRLCSGRSIMGY 60
           MSLPIEQQSPP  GMQQNY GHPGSGSVGPVIGVL+VIIVFAVVAVVVGRLCSGRSIMGY
Sbjct: 1   MSLPIEQQSPP--GMQQNYVGHPGSGSVGPVIGVLIVIIVFAVVAVVVGRLCSGRSIMGY 60

Query: 61  GHFDLESWAETKCSSCIDGRISPPPPRVNVS--AAADSLPAGTMAVRTQQETKQEEPASQ 120
           GHFDLESWAETKCSSCIDGRI+PPPPRV+ S  AAA SLPAGTMAV TQQETKQEEPASQ
Sbjct: 61  GHFDLESWAETKCSSCIDGRITPPPPRVDASTTAAASSLPAGTMAVGTQQETKQEEPASQ 120

Query: 121 NPPANA 125
           NP ANA
Sbjct: 121 NPSANA 124

BLAST of Lsi08G014410 vs. ExPASy TrEMBL
Match: A0A5A7V9H6 (Putative transmembrane protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold43357G00010 PE=4 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 2.7e-51
Identity = 113/125 (90.40%), Postives = 116/125 (92.80%), Query Frame = 0

Query: 1   MSLPIEQQSPPASGMQQNYGGHPGSGSVGPVIGVLVVIIVFAVVAVVVGRLCSGRSIMGY 60
           MS PIEQQSPP  G+QQNY GHPGSGSVGPVIGVLVVIIVFAVVAVVVGRLCSGRSIMGY
Sbjct: 1   MSFPIEQQSPP--GLQQNYVGHPGSGSVGPVIGVLVVIIVFAVVAVVVGRLCSGRSIMGY 60

Query: 61  GHFDLESWAETKCSSCIDGRISPPPPRVNVS-AAADSLPAGTMAVRTQQETKQEEPASQN 120
           GHFDLESWAETKCSSCIDGRI+PPPPRV+ S AAA SLPAGTMAV TQQETKQEEPASQN
Sbjct: 61  GHFDLESWAETKCSSCIDGRITPPPPRVDASTAAASSLPAGTMAVGTQQETKQEEPASQN 120

Query: 121 PPANA 125
           P ANA
Sbjct: 121 PSANA 123

BLAST of Lsi08G014410 vs. ExPASy TrEMBL
Match: A0A6J1CRZ5 (uncharacterized protein LOC111014030 OS=Momordica charantia OX=3673 GN=LOC111014030 PE=4 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 1.3e-48
Identity = 109/124 (87.90%), Postives = 114/124 (91.94%), Query Frame = 0

Query: 1   MSLPIEQQSPPASGMQQNYGGHPGSGSVGPVIGVLVVIIVFAVVAVVVGRLCSGRSIMGY 60
           MSLPIEQQSPPA GMQQ YGGH  +GSVGPVIGVLVVIIVFAVVAVVVGRLCSG++IMGY
Sbjct: 1   MSLPIEQQSPPAPGMQQPYGGHLSNGSVGPVIGVLVVIIVFAVVAVVVGRLCSGKTIMGY 60

Query: 61  GHFDLESWAETKCSSCIDGRISPPPPRVNVSAA--ADSLPAGTMAVRTQQETKQEEPASQ 120
           GHFDLESWAETKCSSCIDGRISPPPPR NVSAA  A +LPA TMAV+TQQETKQEEPASQ
Sbjct: 61  GHFDLESWAETKCSSCIDGRISPPPPRANVSAATTATALPA-TMAVQTQQETKQEEPASQ 120

Query: 121 NPPA 123
           NP A
Sbjct: 121 NPSA 123

BLAST of Lsi08G014410 vs. ExPASy TrEMBL
Match: A0A6J1KK79 (uncharacterized protein LOC111496028 OS=Cucurbita maxima OX=3661 GN=LOC111496028 PE=4 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 3.8e-45
Identity = 104/125 (83.20%), Postives = 108/125 (86.40%), Query Frame = 0

Query: 1   MSLPIEQQSPPASGMQQNYGGHPGS-GSVGPVIGVLVVIIVFAVVAVVVGRLCSGRSIMG 60
           MSLPI+QQSPPA GMQQ YGGH  S GSVGPVIGVLVVIIVFAVVAVVVGRLCSGRSIMG
Sbjct: 1   MSLPIDQQSPPAPGMQQPYGGHASSGGSVGPVIGVLVVIIVFAVVAVVVGRLCSGRSIMG 60

Query: 61  YGHFDLESWAETKCSSCIDGRISPPPPRVNVSAAADSLPAGTMAVRTQQETKQEEPASQN 120
           YGHFDLE WAE KCSSCIDGRIS P PRVNVS AA SLPA TM+V+TQ    Q+EPASQN
Sbjct: 61  YGHFDLERWAEVKCSSCIDGRISSPSPRVNVSTAAASLPAATMSVQTQ----QDEPASQN 120

Query: 121 PPANA 125
           PP NA
Sbjct: 121 PPPNA 121

BLAST of Lsi08G014410 vs. ExPASy TrEMBL
Match: A0A314Z947 (Uncharacterized protein OS=Prunus yedoensis var. nudiflora OX=2094558 GN=Pyn_06388 PE=4 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 2.5e-28
Identity = 75/126 (59.52%), Postives = 91/126 (72.22%), Query Frame = 0

Query: 1   MSLPIEQQSPPASGMQQNYGGHPGSGSVGPVIGVLVVIIVFAVVAVVVGRLCSGRSIMGY 60
           MS+P++QQ PP   +QQN   H   GS+G V+ VLVV+I+  VVAVV+GRLCSGRSIMGY
Sbjct: 1   MSMPLDQQPPPDFELQQNANPHSPHGSIGAVVAVLVVVIILGVVAVVIGRLCSGRSIMGY 60

Query: 61  GHFDLESWAETKCSSCIDGRISPP--PPRVNVSAAADSLPAGTMAVRTQQETKQEEPASQ 120
           GH+DLESWAETKCSSCIDGRI+PP   P V+ S+ + S PA T       E +QEE + Q
Sbjct: 61  GHYDLESWAETKCSSCIDGRINPPLSRPNVSCSSVSTSTPAQT---HQGSELQQEEQSPQ 120

Query: 121 NP-PAN 124
            P PAN
Sbjct: 121 TPQPAN 123

BLAST of Lsi08G014410 vs. NCBI nr
Match: XP_038885752.1 (uncharacterized protein LOC120076044 [Benincasa hispida])

HSP 1 Score: 219.5 bits (558), Expect = 1.6e-53
Identity = 116/126 (92.06%), Postives = 118/126 (93.65%), Query Frame = 0

Query: 1   MSLPIEQQSPPASGMQQNYGGHPGSGSVGPVIGVLVVIIVFAVVAVVVGRLCSGRSIMGY 60
           MSLPIEQQSPPA+ MQQNYGGHPGSGSVGPVIGVLVVIIVFAVVAVVVGRLCSGRSIMGY
Sbjct: 1   MSLPIEQQSPPAAEMQQNYGGHPGSGSVGPVIGVLVVIIVFAVVAVVVGRLCSGRSIMGY 60

Query: 61  GHFDLESWAETKCSSCIDGRISPPPPRVNVSAAADSLPAGTMAVRTQQETK--QEEPASQ 120
           GHFDLESWAETKCSSCIDGRISPPPPRV+  AAA SLPAGTMA R QQETK  QEEPASQ
Sbjct: 61  GHFDLESWAETKCSSCIDGRISPPPPRVDGCAAAASLPAGTMAARPQQETKQEQEEPASQ 120

Query: 121 NPPANA 125
           NPPANA
Sbjct: 121 NPPANA 126

BLAST of Lsi08G014410 vs. NCBI nr
Match: XP_031737057.1 (uncharacterized protein LOC116402140 [Cucumis sativus] >KGN62547.1 hypothetical protein Csa_022283 [Cucumis sativus])

HSP 1 Score: 213.0 bits (541), Expect = 1.5e-51
Identity = 114/126 (90.48%), Postives = 117/126 (92.86%), Query Frame = 0

Query: 1   MSLPIEQQSPPASGMQQNYGGHPGSGSVGPVIGVLVVIIVFAVVAVVVGRLCSGRSIMGY 60
           MSLPIEQQSPP  GMQQNY GHPGSGSVGPVIGVL+VIIVFAVVAVVVGRLCSGRSIMGY
Sbjct: 1   MSLPIEQQSPP--GMQQNYVGHPGSGSVGPVIGVLIVIIVFAVVAVVVGRLCSGRSIMGY 60

Query: 61  GHFDLESWAETKCSSCIDGRISPPPPRVNVS--AAADSLPAGTMAVRTQQETKQEEPASQ 120
           GHFDLESWAETKCSSCIDGRI+PPPPRV+ S  AAA SLPAGTMAV TQQETKQEEPASQ
Sbjct: 61  GHFDLESWAETKCSSCIDGRITPPPPRVDASTTAAASSLPAGTMAVGTQQETKQEEPASQ 120

Query: 121 NPPANA 125
           NP ANA
Sbjct: 121 NPSANA 124

BLAST of Lsi08G014410 vs. NCBI nr
Match: KAA0063096.1 (putative transmembrane protein [Cucumis melo var. makuwa])

HSP 1 Score: 211.1 bits (536), Expect = 5.6e-51
Identity = 113/125 (90.40%), Postives = 116/125 (92.80%), Query Frame = 0

Query: 1   MSLPIEQQSPPASGMQQNYGGHPGSGSVGPVIGVLVVIIVFAVVAVVVGRLCSGRSIMGY 60
           MS PIEQQSPP  G+QQNY GHPGSGSVGPVIGVLVVIIVFAVVAVVVGRLCSGRSIMGY
Sbjct: 1   MSFPIEQQSPP--GLQQNYVGHPGSGSVGPVIGVLVVIIVFAVVAVVVGRLCSGRSIMGY 60

Query: 61  GHFDLESWAETKCSSCIDGRISPPPPRVNVS-AAADSLPAGTMAVRTQQETKQEEPASQN 120
           GHFDLESWAETKCSSCIDGRI+PPPPRV+ S AAA SLPAGTMAV TQQETKQEEPASQN
Sbjct: 61  GHFDLESWAETKCSSCIDGRITPPPPRVDASTAAASSLPAGTMAVGTQQETKQEEPASQN 120

Query: 121 PPANA 125
           P ANA
Sbjct: 121 PSANA 123

BLAST of Lsi08G014410 vs. NCBI nr
Match: KAG6598392.1 (hypothetical protein SDJN03_08170, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 206.1 bits (523), Expect = 1.8e-49
Identity = 111/124 (89.52%), Postives = 113/124 (91.13%), Query Frame = 0

Query: 1   MSLPIEQQSPPASGMQQNYGGHPGSGSVGPVIGVLVVIIVFAVVAVVVGRLCSGRSIMGY 60
           MSLPIEQQSPPA+GM   YGGHP SGSVGPVIGVLVVIIVFAVVAVVVGRLCSG SIMGY
Sbjct: 1   MSLPIEQQSPPATGMLPPYGGHPVSGSVGPVIGVLVVIIVFAVVAVVVGRLCSGMSIMGY 60

Query: 61  GHFDLESWAETKCSSCIDGRISPPPPRVNVSAAADSLPAGTMAVRTQQETKQEEPASQNP 120
           GHFDLESWAETKCSSCIDGRIS  PPRVNVS AA SLP GTMAVRTQQETKQEEPASQNP
Sbjct: 61  GHFDLESWAETKCSSCIDGRISSTPPRVNVSPAA-SLPTGTMAVRTQQETKQEEPASQNP 120

Query: 121 PANA 125
            A+A
Sbjct: 121 TASA 123

BLAST of Lsi08G014410 vs. NCBI nr
Match: XP_022144319.1 (uncharacterized protein LOC111014030 [Momordica charantia])

HSP 1 Score: 202.2 bits (513), Expect = 2.6e-48
Identity = 109/124 (87.90%), Postives = 114/124 (91.94%), Query Frame = 0

Query: 1   MSLPIEQQSPPASGMQQNYGGHPGSGSVGPVIGVLVVIIVFAVVAVVVGRLCSGRSIMGY 60
           MSLPIEQQSPPA GMQQ YGGH  +GSVGPVIGVLVVIIVFAVVAVVVGRLCSG++IMGY
Sbjct: 1   MSLPIEQQSPPAPGMQQPYGGHLSNGSVGPVIGVLVVIIVFAVVAVVVGRLCSGKTIMGY 60

Query: 61  GHFDLESWAETKCSSCIDGRISPPPPRVNVSAA--ADSLPAGTMAVRTQQETKQEEPASQ 120
           GHFDLESWAETKCSSCIDGRISPPPPR NVSAA  A +LPA TMAV+TQQETKQEEPASQ
Sbjct: 61  GHFDLESWAETKCSSCIDGRISPPPPRANVSAATTATALPA-TMAVQTQQETKQEEPASQ 120

Query: 121 NPPA 123
           NP A
Sbjct: 121 NPSA 123

BLAST of Lsi08G014410 vs. TAIR 10
Match: AT2G26520.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G57500.1); Has 51 Blast hits to 51 proteins in 11 species: Archae - 0; Bacteria - 1; Metazoa - 0; Fungi - 0; Plants - 50; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 85.9 bits (211), Expect = 2.5e-17
Identity = 52/138 (37.68%), Postives = 78/138 (56.52%), Query Frame = 0

Query: 2   SLPIEQQSPPASGMQQNYG--GHPGSGSVGPVIGVLVVIIVFAVVAVVVGRLCSGRSIMG 61
           S+P+  Q PPA+ + Q+       G+ ++GP I V +V+ V  V+A V+GRLCSG++I+G
Sbjct: 3   SMPLYWQPPPATEVSQDSSSVSSAGNSTIGPFIAVFIVVTVLCVLASVIGRLCSGKTILG 62

Query: 62  YGHFDLESWAETKCSSCIDGRISP--------PPPRV---NVSAAADSLPAGTMA----- 121
           YG +D+E WAE++C SCIDG I P        PPPR    + S+   +   G +A     
Sbjct: 63  YGDYDMERWAESRCGSCIDGHIHPHRPSPSPTPPPRQPLHHTSSGVSAESEGHVADLDHE 122

BLAST of Lsi08G014410 vs. TAIR 10
Match: AT3G57500.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G26520.1); Has 51 Blast hits to 51 proteins in 11 species: Archae - 0; Bacteria - 1; Metazoa - 0; Fungi - 0; Plants - 50; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 57.0 bits (136), Expect = 1.3e-08
Identity = 37/97 (38.14%), Postives = 48/97 (49.48%), Query Frame = 0

Query: 10  PPASGMQQNYGGHPGSGSVGPVIGVLVVIIVFAVVAVVVGRLCSGRSIMGYGHFDLESWA 69
           PP+     +   H    S+  ++ VL VI + +V+A V  RLC GR +   G  D+E W 
Sbjct: 23  PPSQDQPSHNSDH---RSIETLVVVLAVITILSVLAGVFARLCGGRHLSHGGDHDIEGWV 82

Query: 70  ETKCSSCIDGRI-------SPPPPRVNVSAAADSLPA 100
           E KC SCID  I       SPPPP    +A   S PA
Sbjct: 83  ERKCRSCIDAGIPAVSAAPSPPPPPPPATAEERSKPA 116

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A0A0LP677.1e-5290.48Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G360640 PE=4 SV=1[more]
A0A5A7V9H62.7e-5190.40Putative transmembrane protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s... [more]
A0A6J1CRZ51.3e-4887.90uncharacterized protein LOC111014030 OS=Momordica charantia OX=3673 GN=LOC111014... [more]
A0A6J1KK793.8e-4583.20uncharacterized protein LOC111496028 OS=Cucurbita maxima OX=3661 GN=LOC111496028... [more]
A0A314Z9472.5e-2859.52Uncharacterized protein OS=Prunus yedoensis var. nudiflora OX=2094558 GN=Pyn_063... [more]
Match NameE-valueIdentityDescription
XP_038885752.11.6e-5392.06uncharacterized protein LOC120076044 [Benincasa hispida][more]
XP_031737057.11.5e-5190.48uncharacterized protein LOC116402140 [Cucumis sativus] >KGN62547.1 hypothetical ... [more]
KAA0063096.15.6e-5190.40putative transmembrane protein [Cucumis melo var. makuwa][more]
KAG6598392.11.8e-4989.52hypothetical protein SDJN03_08170, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022144319.12.6e-4887.90uncharacterized protein LOC111014030 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT2G26520.12.5e-1737.68unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT3G57500.11.3e-0838.14unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 103..124
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
NoneNo IPR availablePANTHERPTHR33429:SF24EXPRESSED PROTEINcoord: 1..123
NoneNo IPR availablePANTHERPTHR33429OS02G0708000 PROTEIN-RELATEDcoord: 1..123

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi08G014410.1Lsi08G014410.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane