Lsi08G013810 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi08G013810
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	PfkB domain-containing protein
Location	chr08: 22099858 .. 22102551 (-)
RNA-Seq Expression	Lsi08G013810
Synteny	Lsi08G013810

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGGGTTTTCTTTAATTTGAAATCTATAGCCAGCATGTAGGGAGGAAAATTGACGTTATGTTTCTTTTTTCACCTCTTTATTTGTATTCAAGTGCTGAAATTCGTCTGTTTGAGTGTTTTTTTTTTCAGGTGGGTATGGAAGAGCTTGAGAATATACTGAGGGAGGTGAAATCCTATATACTTTCCTCGCCTGATGTTCTGACTCCGGTTAAGACAATAGCTGGGGGAAGTGTTGCCAACACAATACGAGGATTGAGTGCGGGGTTTGGAGTCAGCTGTGGAATTATTGGCGCCTATGGGGATGATGAGCAAGGAAAATTGTTTGTCTCTAACATGAGCTCCAATGGGGTCAATCTCTCAAGATTGAGGATGATTAAAGGTCCCACAGCTCAGGTCTGCTTCACTCCCTTCATGGAGGGGTGTCCAAATTTGCATTGTGTGTTTATGTCTTTTAAAGTTCTGAGAATGTATTGTAAATTGTACGGCAGTGTGTTTGCCTGGTTGATGCATTGGGCAATCGCACAATGCGACCCTCTCTCGCAAGTGCAGTTAAAGTTCAGGTAACACTACTTTTTTGATTGGGAGTTTGTGTCAGTGTGCGTGTGAGTTTCGAATGCGTTTTGGATAAATTGCTCAGTTTAGTACTTTGTGGTATGCTAAACAGAGGAGATTTGATTACTAAATATACTGACCATCTATTTGTTTTGTCATCTTCAGGGCAATGAACTGACTAGAGATGACTTTAAGGGCTCTAAGGTTAGCGTACTCATATCTTTATTTTCTTTTAACATGTATGACGTGTGTTAAGAAAATAAGAGCGGCCGAAATTGGAAACAGATCAGGGAAACATATTATTATGCTTTTCGGTCTTTCCTGTCCTGTAAAATATTTTCTACTGATTTTATTCTAATTTCCATTGAATCAATTATCAAACTTAAAAGAAATTGGGATGGGGAGGCTTTTGGTAGACTTAATTACTGCCATGTCTTTATTGCAGTGGCTTGTAATGAGATATAGTATATTTAACTTGGAGGTTATTGAAGCGGCTGTAAAGATGGCCAAACAAGAGAGTGTTTTTGTGTCCTTGGATTTGGCCAGTTTTGAGGTACATATTCCTAGACAACATCATTTCTATCATTTGTGTGATTTCAGCTTTGGAGAAGAGGTTGTTATATCATTTGAAAAGAAATGTAAACAAAGTAGCTCACATCTCTATATTTGGCCAGATGGTTCGAGATTTTAGATTACCTCTTCTCCAGCTACTGGAGTCCGGGGACATTGACCTTTGCTTTGCCAATGAAGACGAAGCAAAAGAGTTGATAGGGTGACTGCCTTTGCTTTGAATCTCATTATTCTTCAAACTCAACTGATTTGTAAATTTGAATATCTGCATTACCTCAGCTGTAGGATGTTTCAACTCTTTTGCAAAACGTTGTCTAGTAGATGGCATTAATTTCTGTTACTATATTTAATCTTTACAGGGGTGAGAAAGACGCTGATCCTGAAGTTGCACTAGAATTTCTTGCAAAGCACTGTCAATGGGCTGTCGTGACATTAGGCGCCAATGGTTGTATCGCTAAGCATGGAAAAGAGGTTAGTGATTGATCTTTCCTCCTTTTGGTAACATGTGAAGATTTGATTTTGTGAACAATAGAATATCTTATAAATCGTCTTATGATTGATATATGTGGGGACTGGAGAGGACTGCTTTACTTTAGCTAGGTTCCTTTTCAAGCTTAAACTTAGATCTTTAGCAAAAAGAGAGTTTTTTCGGATAGCTAGTACTTGTGCTTTGCTTTGTTTCGGAATGGGGAGAGGAACAATTTAGCGTTTAGAGGTGTGGAATAGTTTAGGGAGGAAGTTTGTCTGTTGTTAGGTTCTATACCTTCCTCTCACATGATTGGACCACTTTTCTTTAGCTTAGCTTCTTTTTGGGGTTTGTTTTTTTTGTCTGTCCTTTGTATTCTTTCCTTCATTTCAACAGGACGGGAAAGTTTATCGTTGCATCAAGGAAATGTTTTGTTTTATTCTGGTTTCACCTTTTATTTATTTGTTTGGCCCAAAGCGACATACAAAATTCAAGAGATCAAAGAGTGAAACTGAGTTCTGAGATATTATTTCCATGTGGATCATTAGTTATTAAGTTAAATAACTTACCTCAATATATCAGATAGTCCGAGTTCCAGCAATAGGGGAATCAAAGGTGACTGATGCCACTGGAGCAGGTGACTTGTTTGCGAGTGGGTTTTTGTATGGCTTAGTGAAGGGGTTGTCTCTGGAGAAATGCTGTCAGCTTGGATCGTGCAGCGGCGGTTCTGTCATCCGTGCCCTTGGGGGTGAGGTTACCCCAGAAAATTGGCAATGGATGCACAAGCATTTGCAGATCAAAGGGCTTCCTCTCCCTGAGATCCCTCAATGATCAATCATTTTTCTCATGTCGTTGTTGTCCCATCCATTTTCAATGTCTATCAATTTTCTCTTTTTAACAGGCATCTCTTTCAACCTTGGGATGGCTGGACTTTCTTTCAAATTTCAACCCCCTAAAAAAAAAAAGGTTAAATTATTTGAAGTTAGCTGGAGGACCTCTTTGATAATCTCAGGCTTATGTCTCCATCAACAAGATGTAAAAAGTTATCTTTCATTAATGTGATTTCATCTATTTTCAAGCTACTTTTTGGTCCCTAGCTGTGCG

mRNA sequence

ATGTGGGTGGGTATGGAAGAGCTTGAGAATATACTGAGGGAGGTGAAATCCTATATACTTTCCTCGCCTGATGTTCTGACTCCGGTTAAGACAATAGCTGGGGGAAGTGTTGCCAACACAATACGAGGATTGAGTGCGGGGTTTGGAGTCAGCTGTGGAATTATTGGCGCCTATGGGGATGATGAGCAAGGAAAATTGTTTGTCTCTAACATGAGCTCCAATGGGGTCAATCTCTCAAGATTGAGGATGATTAAAGGTCCCACAGCTCAGGTCTGCTTCACTCCCTTCATGGAGGGGTGTCCAAATTTGCATTGTGTGTTTATGTCTTTTAAAGTTCTGAGAATGTATTGTAAATTGTACGGCAGTGTGTTTGCCTGGTTGATGCATTGGGCAATCGCACAATGCGACCCTCTCTCGCAAGTGCAGTTAAAGTTCAGTGTGCGTGTGAGTTTCGAATGCGTTTTGGATAAATTGCTCAGTTTAGTACTTTGTGGTATGCTAAACAGAGGAGATTTGATTACTAAATATACTGACCATCTATTTGGCAATGAACTGACTAGAGATGACTTTAAGGGCTCTAAGTGGCTTGTAATGAGATATAGTATATTTAACTTGGAGGTTATTGAAGCGGCTGTAAAGATGGCCAAACAAGAGATTTTGAGGTACATATTCCTAGACAACATCATTTCTATCATTTGTGTGATTTCAGCTTTGGAGAAGAGGTTGTTATATCATTTGAAAAGAAATGTAAACAAAGTAGCTCACATCTCTATATTTGGCCAGATGGTTCGAGATTTTAGATTACCTCTTCTCCAGCTACTGGAGTCCGGGGACATTGACCTTTGCTTTGCCAATGAAGACGAAGCAAAAGAGTTGATAGGGGGTGAGAAAGACGCTGATCCTGAAGTTGCACTAGAATTTCTTGCAAAGCACTGTCAATGGGCTGTCGTGACATTAGGCGCCAATGGTTGTATCGCTAAGCATGGAAAAGAGATAGTCCGAGTTCCAGCAATAGGGGAATCAAAGGTGACTGATGCCACTGGAGCAGGTGACTTGTTTGCGAGTGGGTTTTTGTATGGCTTAGTGAAGGGGTTGTCTCTGGAGAAATGCTGTCAGCTTGGATCGTGCAGCGGCGGTTCTGTCATCCGTGCCCTTGGGGGTGAGGTTACCCCAGAAAATTGGCAATGGATGCACAAGCATTTGCAGATCAAAGGGCTTCCTCTCCCTGAGATCCCTCAATGATCAATCATTTTTCTCATGTCGTTGTTGTCCCATCCATTTTCAATGTCTATCAATTTTCTCTTTTTAACAGGCATCTCTTTCAACCTTGGGATGGCTGGACTTTCTTTCAAATTTCAACCCCCTAAAAAAAAAAAGGTTAAATTATTTGAAGTTAGCTGGAGGACCTCTTTGATAATCTCAGGCTTATGTCTCCATCAACAAGATGTAAAAAGTTATCTTTCATTAATGTGATTTCATCTATTTTCAAGCTACTTTTTGGTCCCTAGCTGTGCG

Coding sequence (CDS)

ATGTGGGTGGGTATGGAAGAGCTTGAGAATATACTGAGGGAGGTGAAATCCTATATACTTTCCTCGCCTGATGTTCTGACTCCGGTTAAGACAATAGCTGGGGGAAGTGTTGCCAACACAATACGAGGATTGAGTGCGGGGTTTGGAGTCAGCTGTGGAATTATTGGCGCCTATGGGGATGATGAGCAAGGAAAATTGTTTGTCTCTAACATGAGCTCCAATGGGGTCAATCTCTCAAGATTGAGGATGATTAAAGGTCCCACAGCTCAGGTCTGCTTCACTCCCTTCATGGAGGGGTGTCCAAATTTGCATTGTGTGTTTATGTCTTTTAAAGTTCTGAGAATGTATTGTAAATTGTACGGCAGTGTGTTTGCCTGGTTGATGCATTGGGCAATCGCACAATGCGACCCTCTCTCGCAAGTGCAGTTAAAGTTCAGTGTGCGTGTGAGTTTCGAATGCGTTTTGGATAAATTGCTCAGTTTAGTACTTTGTGGTATGCTAAACAGAGGAGATTTGATTACTAAATATACTGACCATCTATTTGGCAATGAACTGACTAGAGATGACTTTAAGGGCTCTAAGTGGCTTGTAATGAGATATAGTATATTTAACTTGGAGGTTATTGAAGCGGCTGTAAAGATGGCCAAACAAGAGATTTTGAGGTACATATTCCTAGACAACATCATTTCTATCATTTGTGTGATTTCAGCTTTGGAGAAGAGGTTGTTATATCATTTGAAAAGAAATGTAAACAAAGTAGCTCACATCTCTATATTTGGCCAGATGGTTCGAGATTTTAGATTACCTCTTCTCCAGCTACTGGAGTCCGGGGACATTGACCTTTGCTTTGCCAATGAAGACGAAGCAAAAGAGTTGATAGGGGGTGAGAAAGACGCTGATCCTGAAGTTGCACTAGAATTTCTTGCAAAGCACTGTCAATGGGCTGTCGTGACATTAGGCGCCAATGGTTGTATCGCTAAGCATGGAAAAGAGATAGTCCGAGTTCCAGCAATAGGGGAATCAAAGGTGACTGATGCCACTGGAGCAGGTGACTTGTTTGCGAGTGGGTTTTTGTATGGCTTAGTGAAGGGGTTGTCTCTGGAGAAATGCTGTCAGCTTGGATCGTGCAGCGGCGGTTCTGTCATCCGTGCCCTTGGGGGTGAGGTTACCCCAGAAAATTGGCAATGGATGCACAAGCATTTGCAGATCAAAGGGCTTCCTCTCCCTGAGATCCCTCAATGA

Protein sequence

MWVGMEELENILREVKSYILSSPDVLTPVKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDEQGKLFVSNMSSNGVNLSRLRMIKGPTAQVCFTPFMEGCPNLHCVFMSFKVLRMYCKLYGSVFAWLMHWAIAQCDPLSQVQLKFSVRVSFECVLDKLLSLVLCGMLNRGDLITKYTDHLFGNELTRDDFKGSKWLVMRYSIFNLEVIEAAVKMAKQEILRYIFLDNIISIICVISALEKRLLYHLKRNVNKVAHISIFGQMVRDFRLPLLQLLESGDIDLCFANEDEAKELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKVTDATGAGDLFASGFLYGLVKGLSLEKCCQLGSCSGGSVIRALGGEVTPENWQWMHKHLQIKGLPLPEIPQ

Homology

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BLAST of Lsi08G013810 vs. ExPASy Swiss-Prot
Match: D9TT10 (ATP-dependent 6-phosphofructokinase OS=Thermoanaerobacterium thermosaccharolyticum (strain ATCC 7956 / DSM 571 / NCIMB 9385 / NCA 3814 / NCTC 13789 / WDCM 00135 / 2032) OX=580327 GN=pfkB PE=1 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 5.1e-11
Identity = 39/111 (35.14%), Postives = 62/111 (55.86%), Query Frame = 0


Query: 279 IDLCFANEDEAKELIGGEKDADPEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAI 338
           +D+   +ED+A+ + G  KD       +FL    +  ++TLG +G I  +G+E +R   +
Sbjct: 197 VDIIKPSEDDAERIFG--KDTPENQLKKFLDLGAKLVILTLGKDGAIVSNGEETIRFNTL 256

Query: 339 GESKVTDATGAGDLFASGFLYGLVKGLSLEKCCQLGSCSGGSVIRALGGEV 390
            + +V D TGAGD F SGF  GL+KG +L+K  +LG       +R +G  V
Sbjct: 257 AD-EVVDTTGAGDAFWSGFYSGLIKGYTLKKSLELGFAVSAYKLRYVGAIV 304

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BLAST of Lsi08G013810 vs. ExPASy Swiss-Prot
Match: Q55480 (Uncharacterized sugar kinase slr0537 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=slr0537 PE=3 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 1.5e-10
Identity = 49/143 (34.27%), Postives = 73/143 (51.05%), Query Frame = 0


Query: 262 MVRDFRLPLLQLLESGDIDLCFANEDEAKELIGGEKDADPEVALEFLAKHCQWAVVTLGA 321
           M + F+  L ++L SG +DL FANE EA E+ G    +D   A+ +     +   +T G 
Sbjct: 196 MAKFFQDGLKEMLGSG-VDLLFANEAEALEMAG---TSDLNQAIAYCKSIAKNFALTRGG 255

Query: 322 NGCIAKHGKEIVRVPAIGESKV--TDATGAGDLFASGFLYGLVKGLSLEKCCQLGSCSGG 381
            G +   G+ ++    IG  KV   D  GAGD++A GFLYGL  G+  EK  QL S +  
Sbjct: 256 AGSLIFDGENLL---TIGTPKVQPIDTVGAGDMYAGGFLYGLTHGMDYEKAGQLASETAA 315

Query: 382 SVIRALGGEVTPENWQWMHKHLQ 403
            V+   G  +  E  Q + + +Q
Sbjct: 316 KVVTCYGPRLDTEILQEILQSVQ 331

[Close]

BLAST of Lsi08G013810 vs. ExPASy Swiss-Prot
Match: Q9CF42 (Ribokinase OS=Lactococcus lactis subsp. lactis (strain IL1403) OX=272623 GN=rbsK PE=3 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 2.2e-09
Identity = 40/111 (36.04%), Postives = 58/111 (52.25%), Query Frame = 0


Query: 279 IDLCFANEDEAKELIGGEKDADPEVAL-EFLAKHCQWAVVTLGANGCIAKHGKEIVRVPA 338
           +D    NE E KEL        P +AL E L K+    +VTLG+ G I   G+ + ++PA
Sbjct: 177 VDYITPNEHECKELF-------PNLALEEILKKYSNRLIVTLGSEGVIFHDGETLQKIPA 236

Query: 339 IGESKVTDATGAGDLFASGFLYGLVKGLSLEKCCQLGSCSGGSVIRALGGE 389
           I ++KV D TGAGD F   F +GL + LS+    +L   +    I+  G +
Sbjct: 237 I-KAKVVDTTGAGDTFNGAFAFGLTENLSISDSIRLAVVASHLSIQKFGAQ 279

[Close]

BLAST of Lsi08G013810 vs. ExPASy Swiss-Prot
Match: Q57849 (Nucleoside kinase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=MJ0406 PE=1 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 1.4e-08
Identity = 30/87 (34.48%), Postives = 46/87 (52.87%), Query Frame = 0


Query: 316 VVTLGANGCIAKHGKEIVRVPAIGESKVTDATGAGDLFASGFLYGLVKGLSLEKCCQLGS 375
           +VT G+ G +     + + +P I   KV D TGAGD + +GFL   VKG  LEKC  +G+
Sbjct: 212 IVTKGSKGSVIYTKDKKIEIPCIKAGKVIDPTGAGDSYRAGFLSAYVKGYDLEKCGLIGA 271

Query: 376 CSGGSVIRALGGEVTPENWQWMHKHLQ 403
            +   V+ A G +     W  + + L+
Sbjct: 272 ATASFVVEAKGCQTNLPTWDKVVERLE 298

[Close]

BLAST of Lsi08G013810 vs. ExPASy Swiss-Prot
Match: O29891 (Uncharacterized sugar kinase AF_0356 OS=Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126) OX=224325 GN=AF_0356 PE=3 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 9.0e-08
Identity = 31/70 (44.29%), Postives = 40/70 (57.14%), Query Frame = 0


Query: 317 VTLGANGCIAKHGKEIVRVPAIGESKVTDATGAGDLFASGFLYGLVKGLSLEKCCQLGSC 376
           V LG+ GC  K G   V V  +  +KV D TGAGD F +GFLYG +KG  +E+C +LG+ 
Sbjct: 172 VKLGSEGCWIKKGDREVTVKPV-STKVVDTTGAGDAFNAGFLYGYLKGKDIEECGRLGNF 231

Query: 377 SGGSVIRALG 387
                I   G
Sbjct: 232 VAAKCIEKYG 240

[Close]

BLAST of Lsi08G013810 vs. ExPASy TrEMBL
Match: A0A6J1CTD9 (uncharacterized protein LOC111014039 OS=Momordica charantia OX=3673 GN=LOC111014039 PE=4 SV=1)

HSP 1 Score: 492.7 bits (1267), Expect = 1.5e-135
Identity = 277/411 (67.40%), Postives = 292/411 (71.05%), Query Frame = 0


Query: 3   VGMEELENILREVKSYILSSPDVLTPVKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDE 62
           VGMEELE+ILR+VKSYILSSPD LTPVKTIAGGSVANTIRGLSAGFGVSCGIIGA GDDE
Sbjct: 54  VGMEELEHILRDVKSYILSSPDDLTPVKTIAGGSVANTIRGLSAGFGVSCGIIGACGDDE 113

Query: 63  QGKLFVSNMSSNGVNLSRLRMIKGPTAQVCFTPFMEGCPNLHCVFMSFKVLRMYCKLYGS 122
           QGKLFV NMSSNGVNLSRLRM KG TAQ              CV                
Sbjct: 114 QGKLFVCNMSSNGVNLSRLRMTKGSTAQ--------------CV---------------- 173

Query: 123 VFAWLMHWAIAQCDPLSQVQLKFSVRVSFECVLDKLLSLVLCGMLNRGDLITKYTDHLFG 182
                                         C++D L +  +   L+    +        G
Sbjct: 174 ------------------------------CLVDALGNRTMRPSLSSAVKVQ-------G 233

Query: 183 NELTRDDFKGSKWLVMRYSIFNLEVIEAAVKMAKQEILRYIFLDNIISIICVISALEKRL 242
           NELTRDDFKGSKWLVMRYSIFNLEVI+AAVKMAKQE + ++ LD                
Sbjct: 234 NELTRDDFKGSKWLVMRYSIFNLEVIQAAVKMAKQEKV-FVSLD---------------- 293

Query: 243 LYHLKRNVNKVAHISIFGQMVRDFRLPLLQLLESGDIDLCFANEDEAKELIGGEKDADPE 302
                        ++ F +MVRDFRLPLLQLLESGDIDLCFANEDEAKELIGGEKDADPE
Sbjct: 294 -------------LASF-EMVRDFRLPLLQLLESGDIDLCFANEDEAKELIGGEKDADPE 353

Query: 303 VALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKVTDATGAGDLFASGFLYGLV 362
           VALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESK TDATGAGDLFASGFLYGLV
Sbjct: 354 VALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKATDATGAGDLFASGFLYGLV 366

Query: 363 KGLSLEKCCQLGSCSGGSVIRALGGEVTPENWQWMHKHLQIKGLPLPEIPQ 414
           KGLSLEKCCQLGSCSGGSVIRALGGEVTPENWQWMHKHLQIKGLPLPEIPQ
Sbjct: 414 KGLSLEKCCQLGSCSGGSVIRALGGEVTPENWQWMHKHLQIKGLPLPEIPQ 366

[Close]

BLAST of Lsi08G013810 vs. ExPASy TrEMBL
Match: A0A6J1GJH7 (uncharacterized protein LOC111454413 OS=Cucurbita moschata OX=3662 GN=LOC111454413 PE=4 SV=1)

HSP 1 Score: 492.7 bits (1267), Expect = 1.5e-135
Identity = 275/413 (66.59%), Postives = 293/413 (70.94%), Query Frame = 0


Query: 1   MWVGMEELENILREVKSYILSSPDVLTPVKTIAGGSVANTIRGLSAGFGVSCGIIGAYGD 60
           M VGMEELENILREVKSYILSSPDVLTP KTIAGGSVANTIRGLSAGFGVSCGIIGAYGD
Sbjct: 51  MPVGMEELENILREVKSYILSSPDVLTPFKTIAGGSVANTIRGLSAGFGVSCGIIGAYGD 110

Query: 61  DEQGKLFVSNMSSNGVNLSRLRMIKGPTAQVCFTPFMEGCPNLHCVFMSFKVLRMYCKLY 120
           D+QGKLFV NMSSNGVNLSRLRMIKGPTAQ              CV              
Sbjct: 111 DQQGKLFVCNMSSNGVNLSRLRMIKGPTAQ--------------CV-------------- 170

Query: 121 GSVFAWLMHWAIAQCDPLSQVQLKFSVRVSFECVLDKLLSLVLCGMLNRGDLITKYTDHL 180
                                           C++D + +  +   L+    +       
Sbjct: 171 --------------------------------CLVDAMGNRTMRPSLSSAVKVQ------ 230

Query: 181 FGNELTRDDFKGSKWLVMRYSIFNLEVIEAAVKMAKQEILRYIFLDNIISIICVISALEK 240
             NELTRDDFKG+KWLVMRYSIFN+EVIEAAVKMAKQE + ++ LD              
Sbjct: 231 -ANELTRDDFKGAKWLVMRYSIFNMEVIEAAVKMAKQEKV-FVSLD-------------- 290

Query: 241 RLLYHLKRNVNKVAHISIFGQMVRDFRLPLLQLLESGDIDLCFANEDEAKELIGGEKDAD 300
                          ++ F +MVRDFR PLLQLLESGDIDLCFANEDEAKELIGGEKDAD
Sbjct: 291 ---------------LASF-EMVRDFRSPLLQLLESGDIDLCFANEDEAKELIGGEKDAD 350

Query: 301 PEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKVTDATGAGDLFASGFLYG 360
           PEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESK TDATGAGDLFASGFLYG
Sbjct: 351 PEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKATDATGAGDLFASGFLYG 365

Query: 361 LVKGLSLEKCCQLGSCSGGSVIRALGGEVTPENWQWMHKHLQIKGLPLPEIPQ 414
           LVKGLSLEKCCQLGSCSGGSVIR+LGGEVTPENWQWMHKHLQIKGLP+ EIPQ
Sbjct: 411 LVKGLSLEKCCQLGSCSGGSVIRSLGGEVTPENWQWMHKHLQIKGLPVQEIPQ 365

[Close]

BLAST of Lsi08G013810 vs. ExPASy TrEMBL
Match: A0A6J1KQC1 (uncharacterized protein LOC111496250 OS=Cucurbita maxima OX=3661 GN=LOC111496250 PE=4 SV=1)

HSP 1 Score: 491.5 bits (1264), Expect = 3.4e-135
Identity = 274/413 (66.34%), Postives = 293/413 (70.94%), Query Frame = 0


Query: 1   MWVGMEELENILREVKSYILSSPDVLTPVKTIAGGSVANTIRGLSAGFGVSCGIIGAYGD 60
           M VGMEELENILREVKSYILSSPDVLTP KTIAGGSVANTIRGLSAGFGVSCGIIGAYGD
Sbjct: 51  MPVGMEELENILREVKSYILSSPDVLTPFKTIAGGSVANTIRGLSAGFGVSCGIIGAYGD 110

Query: 61  DEQGKLFVSNMSSNGVNLSRLRMIKGPTAQVCFTPFMEGCPNLHCVFMSFKVLRMYCKLY 120
           D+QGKLFV NMSSNGVNLSRLRMIKGPTAQ              CV              
Sbjct: 111 DQQGKLFVCNMSSNGVNLSRLRMIKGPTAQ--------------CV-------------- 170

Query: 121 GSVFAWLMHWAIAQCDPLSQVQLKFSVRVSFECVLDKLLSLVLCGMLNRGDLITKYTDHL 180
                                           C++D + +  +   L+    +       
Sbjct: 171 --------------------------------CLVDAMGNRTMRPSLSSAVKVQ------ 230

Query: 181 FGNELTRDDFKGSKWLVMRYSIFNLEVIEAAVKMAKQEILRYIFLDNIISIICVISALEK 240
             NELTRDDFKG+KWLVMRYSIFN+EVIEAAVKMAKQ+ + ++ LD              
Sbjct: 231 -ANELTRDDFKGAKWLVMRYSIFNMEVIEAAVKMAKQDKV-FVSLD-------------- 290

Query: 241 RLLYHLKRNVNKVAHISIFGQMVRDFRLPLLQLLESGDIDLCFANEDEAKELIGGEKDAD 300
                          ++ F +MVRDFR PLLQLLESGDIDLCFANEDEAKELIGGEKDAD
Sbjct: 291 ---------------LASF-EMVRDFRSPLLQLLESGDIDLCFANEDEAKELIGGEKDAD 350

Query: 301 PEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKVTDATGAGDLFASGFLYG 360
           PEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESK TDATGAGDLFASGFLYG
Sbjct: 351 PEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKATDATGAGDLFASGFLYG 365

Query: 361 LVKGLSLEKCCQLGSCSGGSVIRALGGEVTPENWQWMHKHLQIKGLPLPEIPQ 414
           LVKGLSLEKCCQLGSCSGGSVIR+LGGEVTPENWQWMHKHLQIKGLP+ EIPQ
Sbjct: 411 LVKGLSLEKCCQLGSCSGGSVIRSLGGEVTPENWQWMHKHLQIKGLPVQEIPQ 365

[Close]

BLAST of Lsi08G013810 vs. ExPASy TrEMBL
Match: A0A0A0LPB8 (PfkB domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G361810 PE=4 SV=1)

HSP 1 Score: 488.4 bits (1256), Expect = 2.9e-134
Identity = 278/414 (67.15%), Postives = 293/414 (70.77%), Query Frame = 0


Query: 1   MWVGMEELENILREVKSYILSSPDVL-TPVKTIAGGSVANTIRGLSAGFGVSCGIIGAYG 60
           M VGMEELENILREVKSYILSSPD L TPVKTIAGGSVANTIRGLSAGFG+SCGIIGA G
Sbjct: 50  MPVGMEELENILREVKSYILSSPDDLTTPVKTIAGGSVANTIRGLSAGFGISCGIIGACG 109

Query: 61  DDEQGKLFVSNMSSNGVNLSRLRMIKGPTAQVCFTPFMEGCPNLHCVFMSFKVLRMYCKL 120
           DDEQGKLFVSNMSS+GVNLSRLRMIKGPTAQ              CV             
Sbjct: 110 DDEQGKLFVSNMSSHGVNLSRLRMIKGPTAQ--------------CV------------- 169

Query: 121 YGSVFAWLMHWAIAQCDPLSQVQLKFSVRVSFECVLDKLLSLVLCGMLNRGDLITKYTDH 180
                                            C++D L +  +   L+    +      
Sbjct: 170 ---------------------------------CLVDALGNRTMRPSLSSAVKVQ----- 229

Query: 181 LFGNELTRDDFKGSKWLVMRYSIFNLEVIEAAVKMAKQEILRYIFLDNIISIICVISALE 240
             GNELTRDDFKGSKWLVMRYSIFN+EVIEAAVKMAKQE + ++ LD             
Sbjct: 230 --GNELTRDDFKGSKWLVMRYSIFNIEVIEAAVKMAKQEKV-FVSLD------------- 289

Query: 241 KRLLYHLKRNVNKVAHISIFGQMVRDFRLPLLQLLESGDIDLCFANEDEAKELIGGEKDA 300
                           ++ F +MVRDFRLPLLQLLESGDIDLCFANEDEAKELI GEKDA
Sbjct: 290 ----------------LASF-EMVRDFRLPLLQLLESGDIDLCFANEDEAKELIRGEKDA 349

Query: 301 DPEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKVTDATGAGDLFASGFLY 360
           DPEVALEFLAKHCQWA VTLGANGCIAKHGKEIVRVPAIGESK TDATGAGDLFASGFLY
Sbjct: 350 DPEVALEFLAKHCQWAAVTLGANGCIAKHGKEIVRVPAIGESKATDATGAGDLFASGFLY 365

Query: 361 GLVKGLSLEKCCQLGSCSGGSVIRALGGEVTPENWQWMHKHLQIKGLPLPEIPQ 414
           GLVKGLSLEKCCQLGSCSGGSVIRALGGEVTPENWQWMHKHLQIKGLPLPEI Q
Sbjct: 410 GLVKGLSLEKCCQLGSCSGGSVIRALGGEVTPENWQWMHKHLQIKGLPLPEIRQ 365

[Close]

BLAST of Lsi08G013810 vs. ExPASy TrEMBL
Match: A0A6J1HF87 (uncharacterized protein LOC111462499 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111462499 PE=4 SV=1)

HSP 1 Score: 485.3 bits (1248), Expect = 2.5e-133
Identity = 274/413 (66.34%), Postives = 291/413 (70.46%), Query Frame = 0


Query: 1   MWVGMEELENILREVKSYILSSPDVLTPVKTIAGGSVANTIRGLSAGFGVSCGIIGAYGD 60
           M VGMEELENIL EVKSYI SSPD LTPVKTIAGGSVANTIRGLSAGFGVSCGIIGAYGD
Sbjct: 50  MPVGMEELENILSEVKSYIRSSPDDLTPVKTIAGGSVANTIRGLSAGFGVSCGIIGAYGD 109

Query: 61  DEQGKLFVSNMSSNGVNLSRLRMIKGPTAQVCFTPFMEGCPNLHCVFMSFKVLRMYCKLY 120
           DEQGKLFVSNM SNGVNLSRLRMIKGPTAQ              CV              
Sbjct: 110 DEQGKLFVSNMGSNGVNLSRLRMIKGPTAQ--------------CV-------------- 169

Query: 121 GSVFAWLMHWAIAQCDPLSQVQLKFSVRVSFECVLDKLLSLVLCGMLNRGDLITKYTDHL 180
                                           C++D L +  +   L+    +       
Sbjct: 170 --------------------------------CLVDSLGNRTMRPCLSSAVKVQ------ 229

Query: 181 FGNELTRDDFKGSKWLVMRYSIFNLEVIEAAVKMAKQEILRYIFLDNIISIICVISALEK 240
            GNEL+RDDFKGSKWLVMRYSIF+LEVI+AAVKMAKQE                      
Sbjct: 230 -GNELSRDDFKGSKWLVMRYSIFSLEVIQAAVKMAKQE---------------------- 289

Query: 241 RLLYHLKRNVNKVAHISIFGQMVRDFRLPLLQLLESGDIDLCFANEDEAKELIGGEKDAD 300
           R+L  L         ++ F +MVR+FRLPLL+LLESGDIDLCFANEDEAKELIGGEKDAD
Sbjct: 290 RVLVSL--------DLASF-EMVRNFRLPLLKLLESGDIDLCFANEDEAKELIGGEKDAD 349

Query: 301 PEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKVTDATGAGDLFASGFLYG 360
           PEVALEFLAKHCQWAVVTLGANGCIAKHGKE+VR+PAIGESK TDATGAGDLFASGFLYG
Sbjct: 350 PEVALEFLAKHCQWAVVTLGANGCIAKHGKEVVRIPAIGESKATDATGAGDLFASGFLYG 364

Query: 361 LVKGLSLEKCCQLGSCSGGSVIRALGGEVTPENWQWMHKHLQIKGLPLPEIPQ 414
           LVKGLSL KCCQLGSCSGGSVIRALGGEVTPEN QWMHKHLQIKGLPLPEIPQ
Sbjct: 410 LVKGLSLVKCCQLGSCSGGSVIRALGGEVTPENRQWMHKHLQIKGLPLPEIPQ 364

[Close]

BLAST of Lsi08G013810 vs. NCBI nr
Match: XP_038886551.1 (uncharacterized sugar kinase slr0537 [Benincasa hispida])

HSP 1 Score: 496.9 bits (1278), Expect = 1.7e-136
Identity = 281/414 (67.87%), Postives = 295/414 (71.26%), Query Frame = 0


Query: 1   MWVGMEELENILREVKSYILSSP-DVLTPVKTIAGGSVANTIRGLSAGFGVSCGIIGAYG 60
           M VGMEELENILREVKSY LS+P D LTPVKTIAGGSVANTIRGLSAGFGVSCGIIGAYG
Sbjct: 50  MPVGMEELENILREVKSYTLSTPDDDLTPVKTIAGGSVANTIRGLSAGFGVSCGIIGAYG 109

Query: 61  DDEQGKLFVSNMSSNGVNLSRLRMIKGPTAQVCFTPFMEGCPNLHCVFMSFKVLRMYCKL 120
           DDEQGKLFVSNMSSNGVNLSRLRMIKGPTAQ              CV             
Sbjct: 110 DDEQGKLFVSNMSSNGVNLSRLRMIKGPTAQ--------------CV------------- 169

Query: 121 YGSVFAWLMHWAIAQCDPLSQVQLKFSVRVSFECVLDKLLSLVLCGMLNRGDLITKYTDH 180
                                            C++D L +  +   L+    +      
Sbjct: 170 ---------------------------------CLVDALGNRTMRPCLSSAVKVQ----- 229

Query: 181 LFGNELTRDDFKGSKWLVMRYSIFNLEVIEAAVKMAKQEILRYIFLDNIISIICVISALE 240
             GNELTRDDFKGSKWLVMRYSIFN+EVIEAAVKMAKQE + ++ LD             
Sbjct: 230 --GNELTRDDFKGSKWLVMRYSIFNMEVIEAAVKMAKQEKV-FVSLD------------- 289

Query: 241 KRLLYHLKRNVNKVAHISIFGQMVRDFRLPLLQLLESGDIDLCFANEDEAKELIGGEKDA 300
                           ++ F +MVRDFRLPLLQLLESGDIDLCFANEDEAKELIGGE DA
Sbjct: 290 ----------------LASF-EMVRDFRLPLLQLLESGDIDLCFANEDEAKELIGGETDA 349

Query: 301 DPEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKVTDATGAGDLFASGFLY 360
           DPEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESK TDATGAGDLFASGFLY
Sbjct: 350 DPEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKATDATGAGDLFASGFLY 365

Query: 361 GLVKGLSLEKCCQLGSCSGGSVIRALGGEVTPENWQWMHKHLQIKGLPLPEIPQ 414
           GLVKGLSLEKCCQLGSCSGGSVIRALGGEVTPENWQWMHKHLQIKGLPLPEIPQ
Sbjct: 410 GLVKGLSLEKCCQLGSCSGGSVIRALGGEVTPENWQWMHKHLQIKGLPLPEIPQ 365

[Close]

BLAST of Lsi08G013810 vs. NCBI nr
Match: XP_022951670.1 (uncharacterized protein LOC111454413 [Cucurbita moschata] >KAG6585583.1 hypothetical protein SDJN03_18316, partial [Cucurbita argyrosperma subsp. sororia] >KAG7020498.1 hypothetical protein SDJN02_17183, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 492.7 bits (1267), Expect = 3.2e-135
Identity = 275/413 (66.59%), Postives = 293/413 (70.94%), Query Frame = 0


Query: 1   MWVGMEELENILREVKSYILSSPDVLTPVKTIAGGSVANTIRGLSAGFGVSCGIIGAYGD 60
           M VGMEELENILREVKSYILSSPDVLTP KTIAGGSVANTIRGLSAGFGVSCGIIGAYGD
Sbjct: 51  MPVGMEELENILREVKSYILSSPDVLTPFKTIAGGSVANTIRGLSAGFGVSCGIIGAYGD 110

Query: 61  DEQGKLFVSNMSSNGVNLSRLRMIKGPTAQVCFTPFMEGCPNLHCVFMSFKVLRMYCKLY 120
           D+QGKLFV NMSSNGVNLSRLRMIKGPTAQ              CV              
Sbjct: 111 DQQGKLFVCNMSSNGVNLSRLRMIKGPTAQ--------------CV-------------- 170

Query: 121 GSVFAWLMHWAIAQCDPLSQVQLKFSVRVSFECVLDKLLSLVLCGMLNRGDLITKYTDHL 180
                                           C++D + +  +   L+    +       
Sbjct: 171 --------------------------------CLVDAMGNRTMRPSLSSAVKVQ------ 230

Query: 181 FGNELTRDDFKGSKWLVMRYSIFNLEVIEAAVKMAKQEILRYIFLDNIISIICVISALEK 240
             NELTRDDFKG+KWLVMRYSIFN+EVIEAAVKMAKQE + ++ LD              
Sbjct: 231 -ANELTRDDFKGAKWLVMRYSIFNMEVIEAAVKMAKQEKV-FVSLD-------------- 290

Query: 241 RLLYHLKRNVNKVAHISIFGQMVRDFRLPLLQLLESGDIDLCFANEDEAKELIGGEKDAD 300
                          ++ F +MVRDFR PLLQLLESGDIDLCFANEDEAKELIGGEKDAD
Sbjct: 291 ---------------LASF-EMVRDFRSPLLQLLESGDIDLCFANEDEAKELIGGEKDAD 350

Query: 301 PEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKVTDATGAGDLFASGFLYG 360
           PEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESK TDATGAGDLFASGFLYG
Sbjct: 351 PEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKATDATGAGDLFASGFLYG 365

Query: 361 LVKGLSLEKCCQLGSCSGGSVIRALGGEVTPENWQWMHKHLQIKGLPLPEIPQ 414
           LVKGLSLEKCCQLGSCSGGSVIR+LGGEVTPENWQWMHKHLQIKGLP+ EIPQ
Sbjct: 411 LVKGLSLEKCCQLGSCSGGSVIRSLGGEVTPENWQWMHKHLQIKGLPVQEIPQ 365

[Close]

BLAST of Lsi08G013810 vs. NCBI nr
Match: XP_022144332.1 (uncharacterized protein LOC111014039 [Momordica charantia])

HSP 1 Score: 492.7 bits (1267), Expect = 3.2e-135
Identity = 277/411 (67.40%), Postives = 292/411 (71.05%), Query Frame = 0


Query: 3   VGMEELENILREVKSYILSSPDVLTPVKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDE 62
           VGMEELE+ILR+VKSYILSSPD LTPVKTIAGGSVANTIRGLSAGFGVSCGIIGA GDDE
Sbjct: 54  VGMEELEHILRDVKSYILSSPDDLTPVKTIAGGSVANTIRGLSAGFGVSCGIIGACGDDE 113

Query: 63  QGKLFVSNMSSNGVNLSRLRMIKGPTAQVCFTPFMEGCPNLHCVFMSFKVLRMYCKLYGS 122
           QGKLFV NMSSNGVNLSRLRM KG TAQ              CV                
Sbjct: 114 QGKLFVCNMSSNGVNLSRLRMTKGSTAQ--------------CV---------------- 173

Query: 123 VFAWLMHWAIAQCDPLSQVQLKFSVRVSFECVLDKLLSLVLCGMLNRGDLITKYTDHLFG 182
                                         C++D L +  +   L+    +        G
Sbjct: 174 ------------------------------CLVDALGNRTMRPSLSSAVKVQ-------G 233

Query: 183 NELTRDDFKGSKWLVMRYSIFNLEVIEAAVKMAKQEILRYIFLDNIISIICVISALEKRL 242
           NELTRDDFKGSKWLVMRYSIFNLEVI+AAVKMAKQE + ++ LD                
Sbjct: 234 NELTRDDFKGSKWLVMRYSIFNLEVIQAAVKMAKQEKV-FVSLD---------------- 293

Query: 243 LYHLKRNVNKVAHISIFGQMVRDFRLPLLQLLESGDIDLCFANEDEAKELIGGEKDADPE 302
                        ++ F +MVRDFRLPLLQLLESGDIDLCFANEDEAKELIGGEKDADPE
Sbjct: 294 -------------LASF-EMVRDFRLPLLQLLESGDIDLCFANEDEAKELIGGEKDADPE 353

Query: 303 VALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKVTDATGAGDLFASGFLYGLV 362
           VALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESK TDATGAGDLFASGFLYGLV
Sbjct: 354 VALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKATDATGAGDLFASGFLYGLV 366

Query: 363 KGLSLEKCCQLGSCSGGSVIRALGGEVTPENWQWMHKHLQIKGLPLPEIPQ 414
           KGLSLEKCCQLGSCSGGSVIRALGGEVTPENWQWMHKHLQIKGLPLPEIPQ
Sbjct: 414 KGLSLEKCCQLGSCSGGSVIRALGGEVTPENWQWMHKHLQIKGLPLPEIPQ 366

[Close]

BLAST of Lsi08G013810 vs. NCBI nr
Match: XP_023002394.1 (uncharacterized protein LOC111496250 [Cucurbita maxima])

HSP 1 Score: 491.5 bits (1264), Expect = 7.1e-135
Identity = 274/413 (66.34%), Postives = 293/413 (70.94%), Query Frame = 0


Query: 1   MWVGMEELENILREVKSYILSSPDVLTPVKTIAGGSVANTIRGLSAGFGVSCGIIGAYGD 60
           M VGMEELENILREVKSYILSSPDVLTP KTIAGGSVANTIRGLSAGFGVSCGIIGAYGD
Sbjct: 51  MPVGMEELENILREVKSYILSSPDVLTPFKTIAGGSVANTIRGLSAGFGVSCGIIGAYGD 110

Query: 61  DEQGKLFVSNMSSNGVNLSRLRMIKGPTAQVCFTPFMEGCPNLHCVFMSFKVLRMYCKLY 120
           D+QGKLFV NMSSNGVNLSRLRMIKGPTAQ              CV              
Sbjct: 111 DQQGKLFVCNMSSNGVNLSRLRMIKGPTAQ--------------CV-------------- 170

Query: 121 GSVFAWLMHWAIAQCDPLSQVQLKFSVRVSFECVLDKLLSLVLCGMLNRGDLITKYTDHL 180
                                           C++D + +  +   L+    +       
Sbjct: 171 --------------------------------CLVDAMGNRTMRPSLSSAVKVQ------ 230

Query: 181 FGNELTRDDFKGSKWLVMRYSIFNLEVIEAAVKMAKQEILRYIFLDNIISIICVISALEK 240
             NELTRDDFKG+KWLVMRYSIFN+EVIEAAVKMAKQ+ + ++ LD              
Sbjct: 231 -ANELTRDDFKGAKWLVMRYSIFNMEVIEAAVKMAKQDKV-FVSLD-------------- 290

Query: 241 RLLYHLKRNVNKVAHISIFGQMVRDFRLPLLQLLESGDIDLCFANEDEAKELIGGEKDAD 300
                          ++ F +MVRDFR PLLQLLESGDIDLCFANEDEAKELIGGEKDAD
Sbjct: 291 ---------------LASF-EMVRDFRSPLLQLLESGDIDLCFANEDEAKELIGGEKDAD 350

Query: 301 PEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKVTDATGAGDLFASGFLYG 360
           PEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESK TDATGAGDLFASGFLYG
Sbjct: 351 PEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKATDATGAGDLFASGFLYG 365

Query: 361 LVKGLSLEKCCQLGSCSGGSVIRALGGEVTPENWQWMHKHLQIKGLPLPEIPQ 414
           LVKGLSLEKCCQLGSCSGGSVIR+LGGEVTPENWQWMHKHLQIKGLP+ EIPQ
Sbjct: 411 LVKGLSLEKCCQLGSCSGGSVIRSLGGEVTPENWQWMHKHLQIKGLPVQEIPQ 365

[Close]

BLAST of Lsi08G013810 vs. NCBI nr
Match: XP_023538348.1 (uncharacterized protein LOC111799162 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 489.6 bits (1259), Expect = 2.7e-134
Identity = 274/413 (66.34%), Postives = 292/413 (70.70%), Query Frame = 0


Query: 1   MWVGMEELENILREVKSYILSSPDVLTPVKTIAGGSVANTIRGLSAGFGVSCGIIGAYGD 60
           M VGMEELENILREVKSYILSSPDVLT  KTIAGGSVANTIRGLSAGFGVSCGIIGAYGD
Sbjct: 51  MPVGMEELENILREVKSYILSSPDVLTSFKTIAGGSVANTIRGLSAGFGVSCGIIGAYGD 110

Query: 61  DEQGKLFVSNMSSNGVNLSRLRMIKGPTAQVCFTPFMEGCPNLHCVFMSFKVLRMYCKLY 120
           D+QGKLFV NMSSNGVNLSRLRMIKGPTAQ              CV              
Sbjct: 111 DQQGKLFVCNMSSNGVNLSRLRMIKGPTAQ--------------CV-------------- 170

Query: 121 GSVFAWLMHWAIAQCDPLSQVQLKFSVRVSFECVLDKLLSLVLCGMLNRGDLITKYTDHL 180
                                           C++D + +  +   L+    +       
Sbjct: 171 --------------------------------CLVDAMGNRTMRPSLSSAVKVQ------ 230

Query: 181 FGNELTRDDFKGSKWLVMRYSIFNLEVIEAAVKMAKQEILRYIFLDNIISIICVISALEK 240
             NELTRDDFKG+KWLVMRYSIFN+EVIEAAVKMAKQE + ++ LD              
Sbjct: 231 -ANELTRDDFKGAKWLVMRYSIFNMEVIEAAVKMAKQEKV-FVSLD-------------- 290

Query: 241 RLLYHLKRNVNKVAHISIFGQMVRDFRLPLLQLLESGDIDLCFANEDEAKELIGGEKDAD 300
                          ++ F +MVRDFR PLLQLLESGDIDLCFANEDEAKELIGGEKDAD
Sbjct: 291 ---------------LASF-EMVRDFRSPLLQLLESGDIDLCFANEDEAKELIGGEKDAD 350

Query: 301 PEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKVTDATGAGDLFASGFLYG 360
           PEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESK TDATGAGDLFASGFLYG
Sbjct: 351 PEVALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKATDATGAGDLFASGFLYG 365

Query: 361 LVKGLSLEKCCQLGSCSGGSVIRALGGEVTPENWQWMHKHLQIKGLPLPEIPQ 414
           LVKGLSLEKCCQLGSCSGGSVIR+LGGEVTPENWQWMHKHLQIKGLP+ EIPQ
Sbjct: 411 LVKGLSLEKCCQLGSCSGGSVIRSLGGEVTPENWQWMHKHLQIKGLPVQEIPQ 365

[Close]

BLAST of Lsi08G013810 vs. TAIR 10
Match: AT1G19600.1 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 352.1 bits (902), Expect = 6.3e-97
Identity = 199/409 (48.66%), Postives = 254/409 (62.10%), Query Frame = 0


Query: 3   VGMEELENILREVKSYILSSPDVLTPVKTIAGGSVANTIRGLSAGFGVSCGIIGAYGDDE 62
           V  +ELE++L+E+ ++I      + P+K +AGGSV NT+RGLS GFGV+ GIIGAYGDDE
Sbjct: 48  VQKDELEHMLKELDAHI-----SVAPLKKMAGGSVTNTVRGLSVGFGVATGIIGAYGDDE 107

Query: 63  QGKLFVSNMSSNGVNLSRLRMIKGPTAQVCFTPFMEGCPNLHCVFMSFKVLRMYCKLYGS 122
           QG+LFVSNM  +GV++SRLR  KG TAQ              CV                
Sbjct: 108 QGQLFVSNMGFSGVSISRLRKKKGSTAQ--------------CV---------------- 167

Query: 123 VFAWLMHWAIAQCDPLSQVQLKFSVRVSFECVLDKLLSLVLCGMLNRGDLITKYTDHLFG 182
                                         C++D   +  +   L+    I         
Sbjct: 168 ------------------------------CLVDDSGNRTMRPCLSSAVKIQ-------A 227

Query: 183 NELTRDDFKGSKWLVMRYSIFNLEVIEAAVKMAKQEILRYIFLDNIISIICVISALEKRL 242
           +EL+++DF GSKWLV+RY++ NL+VI+AA++ AKQE L         S+   +++ E   
Sbjct: 228 DELSKEDFTGSKWLVLRYAVLNLQVIQAAIRFAKQEGL---------SVSLDLASFE--- 287

Query: 243 LYHLKRNVNKVAHISIFGQMVRDFRLPLLQLLESGDIDLCFANEDEAKELIGGEKDADPE 302
                              MVR+ +  L QLLESG+IDLCFANEDEA EL+ GE++A PE
Sbjct: 288 -------------------MVRNSKSELRQLLESGNIDLCFANEDEAAELLRGEQEAGPE 347

Query: 303 VALEFLAKHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKVTDATGAGDLFASGFLYGLV 362
            ALEFL +HC+WAVVTLG+ GCIAKH KE+V + AIGE+  TDATGAGDLFASGFLYGL+
Sbjct: 348 AALEFLGRHCRWAVVTLGSKGCIAKHDKEVVHISAIGETVATDATGAGDLFASGFLYGLI 353

Query: 363 KGLSLEKCCQLGSCSGGSVIRALGGEVTPENWQWMHKHLQIKGLPLPEI 412
           KGLSLE+CC++GSCSGGSVIRALGGEVTPENWQWMHK LQ+KGLP+P+I
Sbjct: 408 KGLSLEECCKVGSCSGGSVIRALGGEVTPENWQWMHKQLQLKGLPVPDI 353

[Close]

BLAST of Lsi08G013810 vs. TAIR 10
Match: AT3G09820.1 (adenosine kinase 1 )

HSP 1 Score: 53.9 bits (128), Expect = 3.5e-07
Identity = 37/125 (29.60%), Postives = 59/125 (47.20%), Query Frame = 0


Query: 279 IDLCFANEDEAK---ELIGGEKDADPEVALEF------LAKHCQWAVVTLGANGCIAKHG 338
           +D  F NE EA+    + G E D   ++A++          + +  V+T GA+  +    
Sbjct: 216 MDYIFGNETEARTFSRVHGWETDDVEQIAIKMSQLPKASGTYKRTTVITQGADPVVVAED 275

Query: 339 KEIVRVPAI--GESKVTDATGAGDLFASGFLYGLVKGLSLEKCCQLGSCSGGSVIRALGG 393
            ++ + P I   + K+ D  GAGD F  GFL  LV G  +E+C + G C   +V+    G
Sbjct: 276 GKVKKYPVIPLPKEKLVDTNGAGDAFVGGFLSQLVHGKGIEECVRAG-CYASNVVIQRSG 335

[Close]

BLAST of Lsi08G013810 vs. TAIR 10
Match: AT3G09820.2 (adenosine kinase 1 )

HSP 1 Score: 53.9 bits (128), Expect = 3.5e-07
Identity = 37/125 (29.60%), Postives = 59/125 (47.20%), Query Frame = 0


Query: 279 IDLCFANEDEAK---ELIGGEKDADPEVALEF------LAKHCQWAVVTLGANGCIAKHG 338
           +D  F NE EA+    + G E D   ++A++          + +  V+T GA+  +    
Sbjct: 174 MDYIFGNETEARTFSRVHGWETDDVEQIAIKMSQLPKASGTYKRTTVITQGADPVVVAED 233

Query: 339 KEIVRVPAI--GESKVTDATGAGDLFASGFLYGLVKGLSLEKCCQLGSCSGGSVIRALGG 393
            ++ + P I   + K+ D  GAGD F  GFL  LV G  +E+C + G C   +V+    G
Sbjct: 234 GKVKKYPVIPLPKEKLVDTNGAGDAFVGGFLSQLVHGKGIEECVRAG-CYASNVVIQRSG 293

[Close]

BLAST of Lsi08G013810 vs. TAIR 10
Match: AT5G03300.1 (adenosine kinase 2 )

HSP 1 Score: 53.1 bits (126), Expect = 6.0e-07
Identity = 36/125 (28.80%), Postives = 60/125 (48.00%), Query Frame = 0


Query: 279 IDLCFANEDEAK---ELIGGEKDADPEVALEF------LAKHCQWAVVTLGANGCIAKHG 338
           +D  F NE EA+    + G E +   ++A++          + +  V+T GA+  +    
Sbjct: 217 MDFVFGNETEARTFSRVHGWETEDVEQIAIKISQLPKATGTYKRTTVITQGADPVVVAED 276

Query: 339 KEIVRVPAI--GESKVTDATGAGDLFASGFLYGLVKGLSLEKCCQLGSCSGGSVIRALGG 393
            ++ + P I   + K+ D  GAGD F  GF+  LVK  S+E+C + G C   +V+    G
Sbjct: 277 GKVKKYPVIPLPKEKLVDTNGAGDAFVGGFMSQLVKEKSIEECVKAG-CYASNVVIQRSG 336

[Close]

BLAST of Lsi08G013810 vs. TAIR 10
Match: AT5G58730.1 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 52.0 bits (123), Expect = 1.3e-06
Identity = 29/77 (37.66%), Postives = 39/77 (50.65%), Query Frame = 0


Query: 310 KHCQWAVVTLGANGCIAKHGKEIVRVPAIGESKVTDATGAGDLFASGFLYGLVKGLSLEK 369
           KH    VVT G  GC   H  + + VP    +K  D TGAGD F  G + GLV+GL++  
Sbjct: 210 KHLCCVVVTNGEKGCRIYHKDDEMTVPPF-LAKQVDPTGAGDSFLGGLIVGLVEGLTVPD 269

Query: 370 CCQLGSCSGGSVIRALG 387
              LG+  G   +  +G
Sbjct: 270 AALLGNLFGSITVEHIG 285

The following BLAST results are available for this feature:

BLAST of Lsi08G013810 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
D9TT10	5.1e-11	35.14	ATP-dependent 6-phosphofructokinase OS=Thermoanaerobacterium thermosaccharolytic...	[more]
Q55480	1.5e-10	34.27	Uncharacterized sugar kinase slr0537 OS=Synechocystis sp. (strain PCC 6803 / Kaz...	[more]
Q9CF42	2.2e-09	36.04	Ribokinase OS=Lactococcus lactis subsp. lactis (strain IL1403) OX=272623 GN=rbsK...	[more]
Q57849	1.4e-08	34.48	Nucleoside kinase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661...	[more]
O29891	9.0e-08	44.29	Uncharacterized sugar kinase AF_0356 OS=Archaeoglobus fulgidus (strain ATCC 4955...	[more]

BLAST of Lsi08G013810 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CTD9	1.5e-135	67.40	uncharacterized protein LOC111014039 OS=Momordica charantia OX=3673 GN=LOC111014...	[more]
A0A6J1GJH7	1.5e-135	66.59	uncharacterized protein LOC111454413 OS=Cucurbita moschata OX=3662 GN=LOC1114544...	[more]
A0A6J1KQC1	3.4e-135	66.34	uncharacterized protein LOC111496250 OS=Cucurbita maxima OX=3661 GN=LOC111496250...	[more]
A0A0A0LPB8	2.9e-134	67.15	PfkB domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G361810 PE=4 S...	[more]
A0A6J1HF87	2.5e-133	66.34	uncharacterized protein LOC111462499 isoform X1 OS=Cucurbita moschata OX=3662 GN...	[more]

BLAST of Lsi08G013810 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038886551.1	1.7e-136	67.87	uncharacterized sugar kinase slr0537 [Benincasa hispida]	[more]
XP_022951670.1	3.2e-135	66.59	uncharacterized protein LOC111454413 [Cucurbita moschata] >KAG6585583.1 hypothet...	[more]
XP_022144332.1	3.2e-135	67.40	uncharacterized protein LOC111014039 [Momordica charantia]	[more]
XP_023002394.1	7.1e-135	66.34	uncharacterized protein LOC111496250 [Cucurbita maxima]	[more]
XP_023538348.1	2.7e-134	66.34	uncharacterized protein LOC111799162 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lsi08G013810 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G19600.1	6.3e-97	48.66	pfkB-like carbohydrate kinase family protein	[more]
AT3G09820.1	3.5e-07	29.60	adenosine kinase 1	[more]
AT3G09820.2	3.5e-07	29.60	adenosine kinase 1	[more]
AT5G03300.1	6.0e-07	28.80	adenosine kinase 2	[more]
AT5G58730.1	1.3e-06	37.66	pfkB-like carbohydrate kinase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011611	Carbohydrate kinase PfkB	PFAM	PF00294	PfkB	coord: 250..386 e-value: 6.6E-27 score: 94.6
IPR029056	Ribokinase-like	GENE3D	3.40.1190.20		coord: 174..390 e-value: 2.6E-36 score: 127.4
IPR029056	Ribokinase-like	GENE3D	3.40.1190.20		coord: 4..99 e-value: 1.5E-11 score: 46.0
IPR029056	Ribokinase-like	SUPERFAMILY	53613	Ribokinase-like	coord: 27..387
None	No IPR available	PANTHER	PTHR43320	SUGAR KINASE	coord: 183..220 coord: 261..404 coord: 3..91
None	No IPR available	PANTHER	PTHR43320:SF1	CARBOHYDRATE KINASE-LIKE	coord: 183..220 coord: 261..404 coord: 3..91
None	No IPR available	CDD	cd01168	adenosine_kinase	coord: 3..386 e-value: 8.52102E-57 score: 187.436

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi08G013810.1	Lsi08G013810.1	mRNA