Lsi08G013580 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi08G013580
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptiondehydration-responsive element-binding protein 2D
Locationchr08: 21900887 .. 21901561 (-)
RNA-Seq ExpressionLsi08G013580
SyntenyLsi08G013580
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGAAATCATCCTCCAACAACAACAACAACAAAAGACCCTCTTCAAGAAAGGGTTGTATGAGAGGCAAAGGAGGCCCTGAAAACGCTTCTTGTACTTATAAAGGTGTTCGTCAAAGAACCTGGGGCAAATGGGTTGCTGAAATTCGAGAGCCAAATCGTGGAGCTCGTCTTTGGCTTGGTACTTTTGATACTTCTCATGATGCTGCTTTGGCTTATGACAATGCTGCTCGTCGTCTTTATGGCTCTGATGCTAAACTCAATCTTCCTCATCTTTCTCCTCTTTCTGCTTCTGCTTCTGCTTCTGCTTCTCTTGCTGCTGCTGCTTCTTCTTCTTCTGATAATGTTGCTGCTAATGCTAATTCAAATGCTATTTCCAATGCCATTGTGGAAGATGTTGCGTCCTCTGTTTCTCATGAAAACAATAACAACACTCTATTGCCTAAGAAGAGATTTAGGGAGGAAGAGGAAGAACAAATTGGGGGGCTTTGGAGGGCTATGAGTATTAATTTAGATGATTCTATTTGGGTTGAAGCAGCTATGTCTTTGGATTTTCCTGTTGTTATGGAACATCATCAGGATCATCCTCATCATCAAGGCTTTTCTGCTGCTAATTTGATGGATACAAATGGAAATGGTGGATGTGGATGGGATACACTTCAATGGTGTTTATGA

mRNA sequence

ATGTTGAAATCATCCTCCAACAACAACAACAACAAAAGACCCTCTTCAAGAAAGGGTTGTATGAGAGGCAAAGGAGGCCCTGAAAACGCTTCTTGTACTTATAAAGGTGTTCGTCAAAGAACCTGGGGCAAATGGGTTGCTGAAATTCGAGAGCCAAATCGTGGAGCTCGTCTTTGGCTTGGTACTTTTGATACTTCTCATGATGCTGCTTTGGCTTATGACAATGCTGCTCGTCGTCTTTATGGCTCTGATGCTAAACTCAATCTTCCTCATCTTTCTCCTCTTTCTGCTTCTGCTTCTGCTTCTGCTTCTCTTGCTGCTGCTGCTTCTTCTTCTTCTGATAATGTTGCTGCTAATGCTAATTCAAATGCTATTTCCAATGCCATTGTGGAAGATGTTGCGTCCTCTGTTTCTCATGAAAACAATAACAACACTCTATTGCCTAAGAAGAGATTTAGGGAGGAAGAGGAAGAACAAATTGGGGGGCTTTGGAGGGCTATGAGTATTAATTTAGATGATTCTATTTGGGTTGAAGCAGCTATGTCTTTGGATTTTCCTGTTGTTATGGAACATCATCAGGATCATCCTCATCATCAAGGCTTTTCTGCTGCTAATTTGATGGATACAAATGGAAATGGTGGATGTGGATGGGATACACTTCAATGGTGTTTATGA

Coding sequence (CDS)

ATGTTGAAATCATCCTCCAACAACAACAACAACAAAAGACCCTCTTCAAGAAAGGGTTGTATGAGAGGCAAAGGAGGCCCTGAAAACGCTTCTTGTACTTATAAAGGTGTTCGTCAAAGAACCTGGGGCAAATGGGTTGCTGAAATTCGAGAGCCAAATCGTGGAGCTCGTCTTTGGCTTGGTACTTTTGATACTTCTCATGATGCTGCTTTGGCTTATGACAATGCTGCTCGTCGTCTTTATGGCTCTGATGCTAAACTCAATCTTCCTCATCTTTCTCCTCTTTCTGCTTCTGCTTCTGCTTCTGCTTCTCTTGCTGCTGCTGCTTCTTCTTCTTCTGATAATGTTGCTGCTAATGCTAATTCAAATGCTATTTCCAATGCCATTGTGGAAGATGTTGCGTCCTCTGTTTCTCATGAAAACAATAACAACACTCTATTGCCTAAGAAGAGATTTAGGGAGGAAGAGGAAGAACAAATTGGGGGGCTTTGGAGGGCTATGAGTATTAATTTAGATGATTCTATTTGGGTTGAAGCAGCTATGTCTTTGGATTTTCCTGTTGTTATGGAACATCATCAGGATCATCCTCATCATCAAGGCTTTTCTGCTGCTAATTTGATGGATACAAATGGAAATGGTGGATGTGGATGGGATACACTTCAATGGTGTTTATGA

Protein sequence

MLKSSSNNNNNKRPSSRKGCMRGKGGPENASCTYKGVRQRTWGKWVAEIREPNRGARLWLGTFDTSHDAALAYDNAARRLYGSDAKLNLPHLSPLSASASASASLAAAASSSSDNVAANANSNAISNAIVEDVASSVSHENNNNTLLPKKRFREEEEEQIGGLWRAMSINLDDSIWVEAAMSLDFPVVMEHHQDHPHHQGFSAANLMDTNGNGGCGWDTLQWCL
Homology
BLAST of Lsi08G013580 vs. ExPASy Swiss-Prot
Match: Q9LQZ2 (Dehydration-responsive element-binding protein 2D OS=Arabidopsis thaliana OX=3702 GN=DREB2D PE=2 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 6.6e-37
Identity = 104/224 (46.43%), Postives = 130/224 (58.04%), Query Frame = 0

Query: 1   MLKSSSNNNNNKRPSSRKGCMRGKGGPENASCTYKGVRQRTWGKWVAEIREPNRGARLWL 60
           M+  ++      + SSRKGCMRGKGGP+NASCTYKGVRQRTWGKWVAEIREPNRGARLWL
Sbjct: 9   MMVGANKKQRTVQASSRKGCMRGKGGPDNASCTYKGVRQRTWGKWVAEIREPNRGARLWL 68

Query: 61  GTFDTSHDAALAYDNAARRLYGSDAKLNLPHLSPLSASASASASLAAAASSSSDNVAANA 120
           GTFDTS +AALAYD+AAR+LYG +A LNLP          +  S    ASS +     ++
Sbjct: 69  GTFDTSREAALAYDSAARKLYGPEAHLNLP---------ESLRSYPKTASSPASQTTPSS 128

Query: 121 NSNAISNAIVEDVASSVSHENNNNTLLPKKRFREEEEEQIGGLWRAMSINL----DDSIW 180
           N+   S++  E   SS       N +    R  EE        W  ++++L    D SIW
Sbjct: 129 NTGGKSSSDSESPCSS-------NEMSSCGRVTEEIS------WEHINVDLPVMDDSSIW 188

Query: 181 VEAAMSLDFPVVMEHHQDHPHHQGFSAANLMDTNGNGG-CGWDT 220
            EA MSL FP V         H+G +  +  DT  +GG   WD+
Sbjct: 189 EEATMSLGFPWV---------HEGDNDISRFDTCISGGYSNWDS 201

BLAST of Lsi08G013580 vs. ExPASy Swiss-Prot
Match: P61827 (Dehydration-responsive element-binding protein 2G OS=Arabidopsis thaliana OX=3702 GN=DREB2G PE=1 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 1.1e-31
Identity = 61/76 (80.26%), Postives = 71/76 (93.42%), Query Frame = 0

Query: 16 SRKGCMRGKGGPENASCTYKGVRQRTWGKWVAEIREPNRGARLWLGTFDTSHDAALAYDN 75
          SRKGCM+GKGGPENA+CT++GVRQRTWGKWVAEIREPNRG RLWLGTF+TS +AA+AYD 
Sbjct: 16 SRKGCMKGKGGPENATCTFRGVRQRTWGKWVAEIREPNRGTRLWLGTFNTSVEAAMAYDE 75

Query: 76 AARRLYGSDAKLNLPH 92
          AA++LYG +AKLNL H
Sbjct: 76 AAKKLYGHEAKLNLVH 91

BLAST of Lsi08G013580 vs. ExPASy Swiss-Prot
Match: Q84ZA1 (Dehydration-responsive element-binding protein 2C OS=Oryza sativa subsp. japonica OX=39947 GN=DREB2C PE=2 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 7.8e-30
Identity = 81/158 (51.27%), Postives = 106/158 (67.09%), Query Frame = 0

Query: 16  SRKGCMRGKGGPENASCTYKGVRQRTWGKWVAEIREPNRGARLWLGTFDTSHDAALAYDN 75
           SRKGCM+GKGGPEN  C ++GVRQRTWGKWVAEIREPNRGARLWLGTF+T+ DAA AYD+
Sbjct: 37  SRKGCMKGKGGPENQRCPFRGVRQRTWGKWVAEIREPNRGARLWLGTFNTALDAARAYDS 96

Query: 76  AARRLYGSDAKLNLPHLSPLSASASASASLAAAASSSSDNVAANANS-NAISNAIVEDVA 135
           AAR LYG  A+LNL     L A+A+A A  AAA  S +   + N +S N+ S    + + 
Sbjct: 97  AARALYGDCARLNL-----LLAAATAGAPPAAATPSVATPCSTNDDSNNSSSTTHQQQLT 156

Query: 136 SSVSHENNNNTLLPKKRFREEEEEQIGGLWRAMSINLD 173
           + +  +++N TL P    +E+ E  +  L +A    L+
Sbjct: 157 TMLQLDDDNYTLQPSSSDQEDFETYVTRLPKAEDFGLE 189

BLAST of Lsi08G013580 vs. ExPASy Swiss-Prot
Match: Q9SIZ0 (Putative dehydration-responsive element-binding protein 2H OS=Arabidopsis thaliana OX=3702 GN=DREB2H PE=5 SV=3)

HSP 1 Score: 128.6 bits (322), Expect = 8.6e-29
Identity = 57/77 (74.03%), Postives = 66/77 (85.71%), Query Frame = 0

Query: 16  SRKGCMRGKGGPENASCTYKGVRQRTWGKWVAEIREPNRGARLWLGTFDTSHDAALAYDN 75
           SRKGCM+GKGGPEN  C Y GVRQRTWGKWVAEIREP RGA+LWLGTF +S++AALAYD 
Sbjct: 49  SRKGCMKGKGGPENGICDYTGVRQRTWGKWVAEIREPGRGAKLWLGTFSSSYEAALAYDE 108

Query: 76  AARRLYGSDAKLNLPHL 93
           A++ +YG  A+LNLP L
Sbjct: 109 ASKAIYGQSARLNLPLL 125

BLAST of Lsi08G013580 vs. ExPASy Swiss-Prot
Match: Q8LFR2 (Dehydration-responsive element-binding protein 2C OS=Arabidopsis thaliana OX=3702 GN=DREB2C PE=2 SV=2)

HSP 1 Score: 126.7 bits (317), Expect = 3.3e-28
Identity = 72/149 (48.32%), Postives = 104/149 (69.80%), Query Frame = 0

Query: 16  SRKGCMRGKGGPENASCTYKGVRQRTWGKWVAEIREPNRGARLWLGTFDTSHDAALAYDN 75
           SRKGCM+GKGGPEN  C Y+GVRQR WGKWVAEIREP+ GARLWLGTF +S++AALAYD 
Sbjct: 54  SRKGCMKGKGGPENGICDYRGVRQRRWGKWVAEIREPDGGARLWLGTFSSSYEAALAYDE 113

Query: 76  AARRLYGSDAKLNLPHLSPLSASASASASLAAAASSSSD--NVAANANSNAIS---NAIV 135
           AA+ +YG  A+LNLP ++  S+S +A+A+++ + ++ SD   V A  ++NA S      +
Sbjct: 114 AAKAIYGQSARLNLPEITNRSSSTAATATVSGSVTAFSDESEVCAREDTNASSGFGQVKL 173

Query: 136 EDVASSVSHENNNNTLLPKKRFREEEEEQ 160
           ED +      +++  +  + + +EE  E+
Sbjct: 174 EDCSDEYVLLDSSQCIKEELKGKEEVREE 202

BLAST of Lsi08G013580 vs. ExPASy TrEMBL
Match: A0A0A0LL46 (AP2/ERF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G363010 PE=4 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 1.8e-66
Identity = 156/226 (69.03%), Postives = 177/226 (78.32%), Query Frame = 0

Query: 1   MLKS-SSNNNNNKRPSSRKGCMRGKGGPENASCTYKGVRQRTWGKWVAEIREPNRGARLW 60
           MLKS SSNN NNK+ SSRKGCMRGKGGPENA CTYKGVRQRTWGKWVAEIREPNRGARLW
Sbjct: 1   MLKSCSSNNKNNKKASSRKGCMRGKGGPENALCTYKGVRQRTWGKWVAEIREPNRGARLW 60

Query: 61  LGTFDTSHDAALAYDNAARRLYGSDAKLNLPHL-SPLSASASASASLAAAASSSSDNVAA 120
           LGTFDTSHDAALAYDNAARRLYGSDAKLNLP + S +S S S+S+S   + ++++++V A
Sbjct: 61  LGTFDTSHDAALAYDNAARRLYGSDAKLNLPLISSSISVSVSSSSSTTTSDNNNNNSVVA 120

Query: 121 NANS-NAISNAIVEDVASSVSHENNNNTLLPKKRFRE-EEEEQIGGLWRAMSINLDDSIW 180
            AN+ N   N  VEDVASS S  NN       KRFRE E++EQIGGLWRAMSI+LDDSIW
Sbjct: 121 TANTYNNDVNPTVEDVASSSSLSNN-------KRFREDEDQEQIGGLWRAMSISLDDSIW 180

Query: 181 VEAAMSLDFPVVMEHHQDHPHHQGFSAANLMDTNGNGGCGWDTLQW 223
           VEAAMSLDFP++ME        Q F + NL+DTN N G    +LQW
Sbjct: 181 VEAAMSLDFPLLME-------QQTFFSPNLVDTNPNAGLD-SSLQW 211

BLAST of Lsi08G013580 vs. ExPASy TrEMBL
Match: A0A5A7VDQ3 (Dehydration-responsive element-binding protein 2D OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G005340 PE=4 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 4.1e-66
Identity = 155/226 (68.58%), Postives = 176/226 (77.88%), Query Frame = 0

Query: 1   MLKS-SSNNNNNKRPSSRKGCMRGKGGPENASCTYKGVRQRTWGKWVAEIREPNRGARLW 60
           MLKS SSNN NNK+ SSRKGCMRGKGGPENASCTYKGVRQRTWGKWVAEIREPNRGARLW
Sbjct: 1   MLKSCSSNNKNNKKASSRKGCMRGKGGPENASCTYKGVRQRTWGKWVAEIREPNRGARLW 60

Query: 61  LGTFDTSHDAALAYDNAARRLYGSDAKLNLPHLSPLSASASASASLAAAASSSSDN---- 120
           LGTFDTS+DAALAYDNAARRLYGSDAKLNLP +     S+S S S+++++S++SDN    
Sbjct: 61  LGTFDTSYDAALAYDNAARRLYGSDAKLNLPLI-----SSSTSVSVSSSSSTTSDNNNNN 120

Query: 121 --VAANANSNAISNAIVEDVASSVSHENNNNTLLPKKRFREEEEEQIGGLWRAMSINLDD 180
             VA  +  N   N +VEDVASS S  +NN      KRFRE+EEEQIGGLWRAMSI+LDD
Sbjct: 121 SVVATASTYNNKVNPMVEDVASSSSSISNN------KRFREDEEEQIGGLWRAMSISLDD 180

Query: 181 SIWVEAAMSLDFPVVMEHHQDHPHHQGFSAANLMDTNGNGGCGWDT 220
           SIWVEAAMSLDFP++ME        Q   + NL+DTN N   GWD+
Sbjct: 181 SIWVEAAMSLDFPLLME-------QQTSFSPNLVDTNPNP--GWDS 206

BLAST of Lsi08G013580 vs. ExPASy TrEMBL
Match: A0A1S3BAM0 (dehydration-responsive element-binding protein 2D OS=Cucumis melo OX=3656 GN=LOC103488010 PE=4 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 4.1e-66
Identity = 155/226 (68.58%), Postives = 176/226 (77.88%), Query Frame = 0

Query: 1   MLKS-SSNNNNNKRPSSRKGCMRGKGGPENASCTYKGVRQRTWGKWVAEIREPNRGARLW 60
           MLKS SSNN NNK+ SSRKGCMRGKGGPENASCTYKGVRQRTWGKWVAEIREPNRGARLW
Sbjct: 1   MLKSCSSNNKNNKKASSRKGCMRGKGGPENASCTYKGVRQRTWGKWVAEIREPNRGARLW 60

Query: 61  LGTFDTSHDAALAYDNAARRLYGSDAKLNLPHLSPLSASASASASLAAAASSSSDN---- 120
           LGTFDTS+DAALAYDNAARRLYGSDAKLNLP +     S+S S S+++++S++SDN    
Sbjct: 61  LGTFDTSYDAALAYDNAARRLYGSDAKLNLPLI-----SSSTSVSVSSSSSTTSDNNNNN 120

Query: 121 --VAANANSNAISNAIVEDVASSVSHENNNNTLLPKKRFREEEEEQIGGLWRAMSINLDD 180
             VA  +  N   N +VEDVASS S  +NN      KRFRE+EEEQIGGLWRAMSI+LDD
Sbjct: 121 SVVATASTYNNKVNPMVEDVASSSSSISNN------KRFREDEEEQIGGLWRAMSISLDD 180

Query: 181 SIWVEAAMSLDFPVVMEHHQDHPHHQGFSAANLMDTNGNGGCGWDT 220
           SIWVEAAMSLDFP++ME        Q   + NL+DTN N   GWD+
Sbjct: 181 SIWVEAAMSLDFPLLME-------QQTSFSPNLVDTNPNP--GWDS 206

BLAST of Lsi08G013580 vs. ExPASy TrEMBL
Match: A0A6J1HEF2 (dehydration-responsive element-binding protein 2D-like OS=Cucurbita moschata OX=3662 GN=LOC111462736 PE=4 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 1.2e-62
Identity = 145/226 (64.16%), Postives = 164/226 (72.57%), Query Frame = 0

Query: 1   MLKSSSNNNNNKRPS---SRKGCMRGKGGPENASCTYKGVRQRTWGKWVAEIREPNRGAR 60
           MLKSS     +   S   SRKGCMRGKGGPENASCTYKGVRQRTWGKWVAEIREPNRGAR
Sbjct: 50  MLKSSKQVKRSPSASHATSRKGCMRGKGGPENASCTYKGVRQRTWGKWVAEIREPNRGAR 109

Query: 61  LWLGTFDTSHDAALAYDNAARRLYGSDAKLNLPHLSPLSASASASASLAAAASSSSDNVA 120
           LWLGTFDTSHDAA+AYDNAARRLYGS+AKLNLPHLSP     +    L  + SSSSD+ A
Sbjct: 110 LWLGTFDTSHDAAMAYDNAARRLYGSEAKLNLPHLSPNLQLHNHPLLLPGSVSSSSDSAA 169

Query: 121 ANANSNAISNAIVEDVASSVSHENNNNTLLPKKRFREEEEEQIGGLWRAMSINLDDSIWV 180
           A           VEDV S  S  ++N++   + RF EEEEE+IGGLWR+MSI+LDDSIW 
Sbjct: 170 AT----------VEDVGSISSAPHDNSS---RMRFGEEEEEEIGGLWRSMSISLDDSIWA 229

Query: 181 EAAMSLDFPVVMEHHQDHPHHQGFSAANLMDTNGNGGCGWDTLQWC 224
           EAAMSLDFPVV +  +     QG  AA+ +D+N     GWDTLQWC
Sbjct: 230 EAAMSLDFPVVEDRDR-----QGVFAADFVDSN-----GWDTLQWC 252

BLAST of Lsi08G013580 vs. ExPASy TrEMBL
Match: A0A6J1K5U2 (dehydration-responsive element-binding protein 2D-like OS=Cucurbita maxima OX=3661 GN=LOC111491948 PE=4 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 1.5e-60
Identity = 145/229 (63.32%), Postives = 165/229 (72.05%), Query Frame = 0

Query: 1   MLKSS---SNNNNNKRPSSRKGCMRGKGGPENASCTYKGVRQRTWGKWVAEIREPNRGAR 60
           MLKSS     + +    +SRKGCMRGKGGPENASCTYKGVRQRTWGKWVAEIREPNRGAR
Sbjct: 50  MLKSSKQVKRSPSTSHATSRKGCMRGKGGPENASCTYKGVRQRTWGKWVAEIREPNRGAR 109

Query: 61  LWLGTFDTSHDAALAYDNAARRLYGSDAKLNLPHLSPLSASASASASLAAAASSSSDNVA 120
           LWLGTFDTSHDAA+AYDNAARRLYGSDAKLNLPHLSP          L  + SSSSD+ A
Sbjct: 110 LWLGTFDTSHDAAMAYDNAARRLYGSDAKLNLPHLSP--NLQHHPLLLPVSVSSSSDSAA 169

Query: 121 ANANSNAISNAIVEDVA--SSVSHENNNNTLLPKKRF-REEEEEQIGGLWRAMSINLDDS 180
           A           VEDV   SSV H+N++     + RF  EEEEE+IGGLW++MSI+LDDS
Sbjct: 170 AT----------VEDVGSISSVPHDNSS-----RMRFGEEEEEEEIGGLWKSMSISLDDS 229

Query: 181 IWVEAAMSLDFPVVMEHHQDHPHHQGFSAANLMDTNGNGGCGWDTLQWC 224
           IW EAAMSLDFPV+ +  +     QG  AA+ +D+N     GWDTLQWC
Sbjct: 230 IWAEAAMSLDFPVMEDRDR-----QGDFAADFVDSN-----GWDTLQWC 251

BLAST of Lsi08G013580 vs. NCBI nr
Match: XP_038884866.1 (dehydration-responsive element-binding protein 2D-like [Benincasa hispida])

HSP 1 Score: 319.3 bits (817), Expect = 2.6e-83
Identity = 176/225 (78.22%), Postives = 192/225 (85.33%), Query Frame = 0

Query: 1   MLKSSSNNNNNKRPSSRKGCMRGKGGPENASCTYKGVRQRTWGKWVAEIREPNRGARLWL 60
           MLKSSS +NNNKRPSSRKGCMRGKGGPENASCTYKGVRQRTWGKWVAEIREPNRGARLWL
Sbjct: 1   MLKSSS-SNNNKRPSSRKGCMRGKGGPENASCTYKGVRQRTWGKWVAEIREPNRGARLWL 60

Query: 61  GTFDTSHDAALAYDNAARRLYGSDAKLNLPHLSPLSASASASASLAAAASSSSDNVAANA 120
           GTFDTSHDAALAYDNAARRLYGSDAKLNLP              ++A+ SSSSD+VAANA
Sbjct: 61  GTFDTSHDAALAYDNAARRLYGSDAKLNLP--------------VSASTSSSSDSVAANA 120

Query: 121 NSNAISNAIVEDVA-SSVSHENNNNTLLPKKRFREEEEEQIGGLWRAMSINLDDSIWVEA 180
           N N   NA+VEDVA SSVS+EN++NT+LPKKRFREEEEEQIGGLWRAMSI++DDS+WVEA
Sbjct: 121 NVNV--NAMVEDVASSSVSNENSSNTVLPKKRFREEEEEQIGGLWRAMSISVDDSLWVEA 180

Query: 181 AMSLDFPVVMEHHQDHPHHQGFSAANLMDTNGNGGCGWDTLQWCL 225
           AMSLDFPVVM+        QG  +ANLMDTNGNG  GWD+LQWCL
Sbjct: 181 AMSLDFPVVMD--------QGCFSANLMDTNGNG--GWDSLQWCL 198

BLAST of Lsi08G013580 vs. NCBI nr
Match: XP_011649643.1 (dehydration-responsive element-binding protein 2D [Cucumis sativus] >KGN62640.1 hypothetical protein Csa_022003 [Cucumis sativus])

HSP 1 Score: 262.3 bits (669), Expect = 3.8e-66
Identity = 156/226 (69.03%), Postives = 177/226 (78.32%), Query Frame = 0

Query: 1   MLKS-SSNNNNNKRPSSRKGCMRGKGGPENASCTYKGVRQRTWGKWVAEIREPNRGARLW 60
           MLKS SSNN NNK+ SSRKGCMRGKGGPENA CTYKGVRQRTWGKWVAEIREPNRGARLW
Sbjct: 1   MLKSCSSNNKNNKKASSRKGCMRGKGGPENALCTYKGVRQRTWGKWVAEIREPNRGARLW 60

Query: 61  LGTFDTSHDAALAYDNAARRLYGSDAKLNLPHL-SPLSASASASASLAAAASSSSDNVAA 120
           LGTFDTSHDAALAYDNAARRLYGSDAKLNLP + S +S S S+S+S   + ++++++V A
Sbjct: 61  LGTFDTSHDAALAYDNAARRLYGSDAKLNLPLISSSISVSVSSSSSTTTSDNNNNNSVVA 120

Query: 121 NANS-NAISNAIVEDVASSVSHENNNNTLLPKKRFRE-EEEEQIGGLWRAMSINLDDSIW 180
            AN+ N   N  VEDVASS S  NN       KRFRE E++EQIGGLWRAMSI+LDDSIW
Sbjct: 121 TANTYNNDVNPTVEDVASSSSLSNN-------KRFREDEDQEQIGGLWRAMSISLDDSIW 180

Query: 181 VEAAMSLDFPVVMEHHQDHPHHQGFSAANLMDTNGNGGCGWDTLQW 223
           VEAAMSLDFP++ME        Q F + NL+DTN N G    +LQW
Sbjct: 181 VEAAMSLDFPLLME-------QQTFFSPNLVDTNPNAGLD-SSLQW 211

BLAST of Lsi08G013580 vs. NCBI nr
Match: XP_008444762.1 (PREDICTED: dehydration-responsive element-binding protein 2D [Cucumis melo] >KAA0065197.1 dehydration-responsive element-binding protein 2D [Cucumis melo var. makuwa])

HSP 1 Score: 261.2 bits (666), Expect = 8.5e-66
Identity = 155/226 (68.58%), Postives = 176/226 (77.88%), Query Frame = 0

Query: 1   MLKS-SSNNNNNKRPSSRKGCMRGKGGPENASCTYKGVRQRTWGKWVAEIREPNRGARLW 60
           MLKS SSNN NNK+ SSRKGCMRGKGGPENASCTYKGVRQRTWGKWVAEIREPNRGARLW
Sbjct: 1   MLKSCSSNNKNNKKASSRKGCMRGKGGPENASCTYKGVRQRTWGKWVAEIREPNRGARLW 60

Query: 61  LGTFDTSHDAALAYDNAARRLYGSDAKLNLPHLSPLSASASASASLAAAASSSSDN---- 120
           LGTFDTS+DAALAYDNAARRLYGSDAKLNLP +     S+S S S+++++S++SDN    
Sbjct: 61  LGTFDTSYDAALAYDNAARRLYGSDAKLNLPLI-----SSSTSVSVSSSSSTTSDNNNNN 120

Query: 121 --VAANANSNAISNAIVEDVASSVSHENNNNTLLPKKRFREEEEEQIGGLWRAMSINLDD 180
             VA  +  N   N +VEDVASS S  +NN      KRFRE+EEEQIGGLWRAMSI+LDD
Sbjct: 121 SVVATASTYNNKVNPMVEDVASSSSSISNN------KRFREDEEEQIGGLWRAMSISLDD 180

Query: 181 SIWVEAAMSLDFPVVMEHHQDHPHHQGFSAANLMDTNGNGGCGWDT 220
           SIWVEAAMSLDFP++ME        Q   + NL+DTN N   GWD+
Sbjct: 181 SIWVEAAMSLDFPLLME-------QQTSFSPNLVDTNPNP--GWDS 206

BLAST of Lsi08G013580 vs. NCBI nr
Match: XP_023546902.1 (dehydration-responsive element-binding protein 2D-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 250.4 bits (638), Expect = 1.5e-62
Identity = 146/228 (64.04%), Postives = 165/228 (72.37%), Query Frame = 0

Query: 1   MLKSSSNNNNNKRPS---SRKGCMRGKGGPENASCTYKGVRQRTWGKWVAEIREPNRGAR 60
           MLKSS     +   S   SRKGCMRGKGGPENASCTYKGVRQRTWGKWVAEIREPNRGAR
Sbjct: 51  MLKSSKQVKRSPSASHATSRKGCMRGKGGPENASCTYKGVRQRTWGKWVAEIREPNRGAR 110

Query: 61  LWLGTFDTSHDAALAYDNAARRLYGSDAKLNLPHLSPLSASASASASLAAAASSSSDNVA 120
           LWLGTFDTSHDAA+AYDNAARRLYGS+AKLNLPHLSP     +    L  + SSSSD+ A
Sbjct: 111 LWLGTFDTSHDAAMAYDNAARRLYGSEAKLNLPHLSPNLQHHNHPLLLPVSVSSSSDSAA 170

Query: 121 ANANSNAISNAIVEDVA--SSVSHENNNNTLLPKKRFREEEEEQIGGLWRAMSINLDDSI 180
           A           VEDV   SS  H+N+++    + RF EEEEE+IGGLWR+MSI+LDDSI
Sbjct: 171 AT----------VEDVGSISSAPHDNSSS----RMRFGEEEEEEIGGLWRSMSISLDDSI 230

Query: 181 WVEAAMSLDFPVVMEHHQDHPHHQGFSAANLMDTNGNGGCGWDTLQWC 224
           W EAAMSLDFPVV +  +     QG  AA+ +D+N     GWDTLQWC
Sbjct: 231 WAEAAMSLDFPVVEDRDR-----QGVFAADFVDSN-----GWDTLQWC 254

BLAST of Lsi08G013580 vs. NCBI nr
Match: KAG6598473.1 (Dehydration-responsive element-binding protein 2D, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 249.6 bits (636), Expect = 2.5e-62
Identity = 145/226 (64.16%), Postives = 164/226 (72.57%), Query Frame = 0

Query: 1   MLKSSSNNNNNKRPS---SRKGCMRGKGGPENASCTYKGVRQRTWGKWVAEIREPNRGAR 60
           MLKSS     +   S   SRKGCMRGKGGPENASCTYKGVRQRTWGKWVAEIREPNRGAR
Sbjct: 50  MLKSSKQVKRSPSASHATSRKGCMRGKGGPENASCTYKGVRQRTWGKWVAEIREPNRGAR 109

Query: 61  LWLGTFDTSHDAALAYDNAARRLYGSDAKLNLPHLSPLSASASASASLAAAASSSSDNVA 120
           LWLGTFDTSHDAA+AYDNAARRLYGS+AKLNLPHLSP     +    L  + SSSSD+ A
Sbjct: 110 LWLGTFDTSHDAAMAYDNAARRLYGSEAKLNLPHLSPNLQLHNHPLLLPGSVSSSSDSAA 169

Query: 121 ANANSNAISNAIVEDVASSVSHENNNNTLLPKKRFREEEEEQIGGLWRAMSINLDDSIWV 180
           A           VEDV S  S  ++N++   + RF EEEEE+IGGLWR+MSI+LDDSIW 
Sbjct: 170 AT----------VEDVGSISSAPHDNSS---RMRFGEEEEEEIGGLWRSMSISLDDSIWA 229

Query: 181 EAAMSLDFPVVMEHHQDHPHHQGFSAANLMDTNGNGGCGWDTLQWC 224
           EAAMSLDFPVV +  +     QG  AA+ +D+N     GWDTLQWC
Sbjct: 230 EAAMSLDFPVVEDRDR-----QGVFAADFVDSN-----GWDTLQWC 252

BLAST of Lsi08G013580 vs. TAIR 10
Match: AT1G75490.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 155.6 bits (392), Expect = 4.7e-38
Identity = 104/224 (46.43%), Postives = 130/224 (58.04%), Query Frame = 0

Query: 1   MLKSSSNNNNNKRPSSRKGCMRGKGGPENASCTYKGVRQRTWGKWVAEIREPNRGARLWL 60
           M+  ++      + SSRKGCMRGKGGP+NASCTYKGVRQRTWGKWVAEIREPNRGARLWL
Sbjct: 9   MMVGANKKQRTVQASSRKGCMRGKGGPDNASCTYKGVRQRTWGKWVAEIREPNRGARLWL 68

Query: 61  GTFDTSHDAALAYDNAARRLYGSDAKLNLPHLSPLSASASASASLAAAASSSSDNVAANA 120
           GTFDTS +AALAYD+AAR+LYG +A LNLP          +  S    ASS +     ++
Sbjct: 69  GTFDTSREAALAYDSAARKLYGPEAHLNLP---------ESLRSYPKTASSPASQTTPSS 128

Query: 121 NSNAISNAIVEDVASSVSHENNNNTLLPKKRFREEEEEQIGGLWRAMSINL----DDSIW 180
           N+   S++  E   SS       N +    R  EE        W  ++++L    D SIW
Sbjct: 129 NTGGKSSSDSESPCSS-------NEMSSCGRVTEEIS------WEHINVDLPVMDDSSIW 188

Query: 181 VEAAMSLDFPVVMEHHQDHPHHQGFSAANLMDTNGNGG-CGWDT 220
            EA MSL FP V         H+G +  +  DT  +GG   WD+
Sbjct: 189 EEATMSLGFPWV---------HEGDNDISRFDTCISGGYSNWDS 201

BLAST of Lsi08G013580 vs. TAIR 10
Match: AT5G18450.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 138.3 bits (347), Expect = 7.7e-33
Identity = 61/76 (80.26%), Postives = 71/76 (93.42%), Query Frame = 0

Query: 16 SRKGCMRGKGGPENASCTYKGVRQRTWGKWVAEIREPNRGARLWLGTFDTSHDAALAYDN 75
          SRKGCM+GKGGPENA+CT++GVRQRTWGKWVAEIREPNRG RLWLGTF+TS +AA+AYD 
Sbjct: 16 SRKGCMKGKGGPENATCTFRGVRQRTWGKWVAEIREPNRGTRLWLGTFNTSVEAAMAYDE 75

Query: 76 AARRLYGSDAKLNLPH 92
          AA++LYG +AKLNL H
Sbjct: 76 AAKKLYGHEAKLNLVH 91

BLAST of Lsi08G013580 vs. TAIR 10
Match: AT2G40350.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 128.6 bits (322), Expect = 6.1e-30
Identity = 57/77 (74.03%), Postives = 66/77 (85.71%), Query Frame = 0

Query: 16  SRKGCMRGKGGPENASCTYKGVRQRTWGKWVAEIREPNRGARLWLGTFDTSHDAALAYDN 75
           SRKGCM+GKGGPEN  C Y GVRQRTWGKWVAEIREP RGA+LWLGTF +S++AALAYD 
Sbjct: 49  SRKGCMKGKGGPENGICDYTGVRQRTWGKWVAEIREPGRGAKLWLGTFSSSYEAALAYDE 108

Query: 76  AARRLYGSDAKLNLPHL 93
           A++ +YG  A+LNLP L
Sbjct: 109 ASKAIYGQSARLNLPLL 125

BLAST of Lsi08G013580 vs. TAIR 10
Match: AT2G40340.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 126.7 bits (317), Expect = 2.3e-29
Identity = 72/149 (48.32%), Postives = 104/149 (69.80%), Query Frame = 0

Query: 16  SRKGCMRGKGGPENASCTYKGVRQRTWGKWVAEIREPNRGARLWLGTFDTSHDAALAYDN 75
           SRKGCM+GKGGPEN  C Y+GVRQR WGKWVAEIREP+ GARLWLGTF +S++AALAYD 
Sbjct: 54  SRKGCMKGKGGPENGICDYRGVRQRRWGKWVAEIREPDGGARLWLGTFSSSYEAALAYDE 113

Query: 76  AARRLYGSDAKLNLPHLSPLSASASASASLAAAASSSSD--NVAANANSNAIS---NAIV 135
           AA+ +YG  A+LNLP ++  S+S +A+A+++ + ++ SD   V A  ++NA S      +
Sbjct: 114 AAKAIYGQSARLNLPEITNRSSSTAATATVSGSVTAFSDESEVCAREDTNASSGFGQVKL 173

Query: 136 EDVASSVSHENNNNTLLPKKRFREEEEEQ 160
           ED +      +++  +  + + +EE  E+
Sbjct: 174 EDCSDEYVLLDSSQCIKEELKGKEEVREE 202

BLAST of Lsi08G013580 vs. TAIR 10
Match: AT3G57600.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 121.7 bits (304), Expect = 7.5e-28
Identity = 55/76 (72.37%), Postives = 64/76 (84.21%), Query Frame = 0

Query: 17 RKGCMRGKGGPENASCTYKGVRQRTWGKWVAEIREPNRGARLWLGTFDTSHDAALAYDNA 76
          +KG  RGKGGP+NA C Y+GVRQRTWGKWVAEIREP + ARLWLG+F T+ +AA+AYD A
Sbjct: 11 KKGPARGKGGPQNALCQYRGVRQRTWGKWVAEIREPKKRARLWLGSFATAEEAAMAYDEA 70

Query: 77 ARRLYGSDAKLNLPHL 93
          A +LYG DA LNLPHL
Sbjct: 71 ALKLYGHDAYLNLPHL 86

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LQZ26.6e-3746.43Dehydration-responsive element-binding protein 2D OS=Arabidopsis thaliana OX=370... [more]
P618271.1e-3180.26Dehydration-responsive element-binding protein 2G OS=Arabidopsis thaliana OX=370... [more]
Q84ZA17.8e-3051.27Dehydration-responsive element-binding protein 2C OS=Oryza sativa subsp. japonic... [more]
Q9SIZ08.6e-2974.03Putative dehydration-responsive element-binding protein 2H OS=Arabidopsis thalia... [more]
Q8LFR23.3e-2848.32Dehydration-responsive element-binding protein 2C OS=Arabidopsis thaliana OX=370... [more]
Match NameE-valueIdentityDescription
A0A0A0LL461.8e-6669.03AP2/ERF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G363010 PE=... [more]
A0A5A7VDQ34.1e-6668.58Dehydration-responsive element-binding protein 2D OS=Cucumis melo var. makuwa OX... [more]
A0A1S3BAM04.1e-6668.58dehydration-responsive element-binding protein 2D OS=Cucumis melo OX=3656 GN=LOC... [more]
A0A6J1HEF21.2e-6264.16dehydration-responsive element-binding protein 2D-like OS=Cucurbita moschata OX=... [more]
A0A6J1K5U21.5e-6063.32dehydration-responsive element-binding protein 2D-like OS=Cucurbita maxima OX=36... [more]
Match NameE-valueIdentityDescription
XP_038884866.12.6e-8378.22dehydration-responsive element-binding protein 2D-like [Benincasa hispida][more]
XP_011649643.13.8e-6669.03dehydration-responsive element-binding protein 2D [Cucumis sativus] >KGN62640.1 ... [more]
XP_008444762.18.5e-6668.58PREDICTED: dehydration-responsive element-binding protein 2D [Cucumis melo] >KAA... [more]
XP_023546902.11.5e-6264.04dehydration-responsive element-binding protein 2D-like [Cucurbita pepo subsp. pe... [more]
KAG6598473.12.5e-6264.16Dehydration-responsive element-binding protein 2D, partial [Cucurbita argyrosper... [more]
Match NameE-valueIdentityDescription
AT1G75490.14.7e-3846.43Integrase-type DNA-binding superfamily protein [more]
AT5G18450.17.7e-3380.26Integrase-type DNA-binding superfamily protein [more]
AT2G40350.16.1e-3074.03Integrase-type DNA-binding superfamily protein [more]
AT2G40340.12.3e-2948.32Integrase-type DNA-binding superfamily protein [more]
AT3G57600.17.5e-2872.37Integrase-type DNA-binding superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001471AP2/ERF domainPRINTSPR00367ETHRSPELEMNTcoord: 34..45
score: 64.7
coord: 56..72
score: 59.36
IPR001471AP2/ERF domainSMARTSM00380rav1_2coord: 33..96
e-value: 4.0E-36
score: 136.0
IPR001471AP2/ERF domainPFAMPF00847AP2coord: 33..82
e-value: 2.8E-14
score: 53.1
IPR001471AP2/ERF domainPROSITEPS51032AP2_ERFcoord: 33..90
score: 22.02294
IPR001471AP2/ERF domainCDDcd00018AP2coord: 32..91
e-value: 6.73126E-30
score: 103.891
IPR036955AP2/ERF domain superfamilyGENE3D3.30.730.10AP2/ERF domaincoord: 33..91
e-value: 1.3E-24
score: 88.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..16
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..32
NoneNo IPR availablePANTHERPTHR31241:SF53DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN 2Dcoord: 9..209
NoneNo IPR availablePANTHERPTHR31241DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN 2Ccoord: 9..209
IPR016177DNA-binding domain superfamilySUPERFAMILY54171DNA-binding domaincoord: 33..91

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi08G013580.1Lsi08G013580.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045893 positive regulation of transcription, DNA-templated
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0000976 transcription cis-regulatory region binding
molecular_function GO:0003677 DNA binding