Lsi08G013060 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi08G013060
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionDNA-directed RNA polymerase subunit
Locationchr08: 21418386 .. 21423084 (+)
RNA-Seq ExpressionLsi08G013060
SyntenyLsi08G013060
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTCAGTAGGCTCAGCCCAATTACATGGCATTTTCGAAAATAATGCAAAACCCTATTTTATGAGAAAAAAGAAAAACGAAAAACAAAAAGCAAAACCCTACTTTTACCTTCTCTCTCCAGGCCATTGTGATTTTTCAGACCCTTCACCTTTCTTGCCGGCTCGTCACTCTCCGCCGCTCGCTGCAGCTGGTCACTGGATCTCGCCCCGACAAGTCGTCTCTAGGTAAAATAGTAAACCATCTTCGTCGTTTCTCTTCCATCAACGTCGACTGAACTTGGTTCAATCGAAAATCTGATTCGAACTTTCGGCTTCCGTCCGGTTCGAGGAGCATCCACACACTCAGCGGTCGGTCCGGTCCAGAATTTCTGTGCGAACCGAACCGAACCAATTTACAGTGCAGCTCCGGAGGGTTTAAGCTTCCAAAAGCCAAAACTCAGGATGAAACCATCTTGTTTTCGTTGTTCTTCTTTAGCAAGTTTTCTCTAAGGTCGATTATTGATCCATATCTCCGCCCAGCTCAGCTGTCTTTGGCTAACTTTTCGACAAGAAATCTGTAAAAGCTCTTTCTTGGACTTCATTAATTTTAGATGGGATCTTGAATAGGGATAGATTACAAGTGAGCTAATTTGAGCTTATCAAACTGATTAAATTGAGCTTACTACTTGTTATGAGGAGGCCCCTTGAGGTTCTGTCTGCAAATTTTTTTGGATATCTGAATGACCCATCTTAGTTATTTCTTTGAGACATTATGTTCGTTTTAGCCTATTGTGGTTATAAGTTCTCTCATTCTTTTTCATTGCTGTTTTAGTTTATTGACTTGTTTCTAGATGAAGAGCCAATTTCTCCTATAACTTTGTTAATTACCTCCAATTATTCTTGGCCTTGCTTCTTCTACAGCCTATTTTATTGGGGAACTCTGTTTACCAAATCCCCTTTTTGCTATCACAATTCGACCACCCTTTTTGGGCTCAACTTTCCTGTCTTTTAGAGTGTGAGCATTTTTTTTTAGTCTCTTATTGTTGTAATTGAACTTCTTATAGTTATTCTAACTTTTCTCTTTTTTTGTGGACATTGTTAGTTAGAAAGCTCTTGGGGAAGCAGCAAGGGCCACTCATGGCTTATTCTCGTGGACATGACTTCTTGTTTTGCAATTTGTGTGGAACCATGATGTCCTTCAGCTCAACGAAATATGTTGAGTGTCCTTTGTGCAAGTCTAGGCGAAGTGCAAAAGGTGGACATACACATTGAATTTAATCTTATTCCATTTTAGTTTTTCATTTTGGATCATTTGAACATTCGAACACTCATTACTTATATGAATGAGTGCTTGATTGGGTATGCCTTGCAGTAATTACACTCTGACTTAATCTTATTATATTGTAGTGTAGACCATTTTTCCTTCAATGTTTATCTGAGTAAAATGTGAAAAGCATCGTTTATTTTTCCATTTCCTATCTCGAGTTTCTATTTTTCTTTTTAATCTTTTAACCAGAACAGATTGAGTCTGGGCTGTGCAGGTTGGGTTTTCTTAACCTGTCATTTTCATTTTAAAAAATTCCTCTTGTAGATAGGAGAGTAAGCGTTTCTATTAGGGAATTTGCATGATCTTCTGCTTTGAGTGTTCAACTTTGAAAGCATGATTTCTTACACAAAAAACATTGTTCAAAATTCATAGTTCACAGGATTTTTATTTGATGAAGGTCACCGCTGTTCGCTTGTGCATTCTGGTTTCAATCAAAGCATAGATAAGTCGACTATTATTATTTTGATGAACAACTAACTAGCTTTCATATATATATATATATAAAGAAAAAAGAAAAAAGAAAAGAAAAGAAAAGGTGGCCACCTACTTAGGATTTAATATCCTACGAGTTTCCTTGACACCCAAATGTTGTAGGGTCAGGCGAGTTGTCCCGTGAGATTAGTCAAGGTGTGCGTAAGTTGGTCCGGACACTCATAGATATGAGAAAAAAAAAAAGACTATCTCGAATAGGACACAAAGCACCTTCCATTCAGATTAAGCAGCCATCTGCGAATAAGAAAAAAAGGGGAGGAAATTTGATCCCTTGATGTAGCCAAGCCTAACCTTTCATCTAGCTTTACTATTCGGAAAAGAACCAAGAAGTCCACAGACGAGAGTCATACTTCAATTCATATTATCCTCCTCTGCCCAACTCATTAGCTGCAAGGATTGAGTCTATAATTTGGAACTGTCCAGCAAAATCCCGGGGAAATTTTGAGACCATTTTAGAGAGGATAGTTTGAACCTATTTACAAACATTTATGAAGAATTTTGTAAGGGCCCATAGTTAGAGAGGCTTATGGGTCTATTATCTTTCACTTCCTTGCCTTCATCCTCCTTAGCCATAAAGGCAATATAAGTCTTGGTTATATTCACATCAAGGATTTCTTTCTTAAAGAAGCCTAGATGTTGACATGGTATCATGCTTGAGAGTGTCCTAAGTTTTTGTGAAGAATTCCAAGGAAAAACCATCTGGACCCATAGCTTGCTTGGCCTTGGCCCTTGACTAAGACTAGAAATAGCCCTTGGAACTTTATGCTAAATAAAAGCCTACAGGAGTAGAGAACCGGCTGCAGAGGTAGAATTCCAACACAGACTATGGAAGATTATGTGGTCCTCTGAGCTTAGTACGGTAGACTGCTGGGGTTCTGACAATTGGAACCTTAAATTCAGATGCAACTCATTGATGTAGGTGAACGCACAGGCTGACTGATGTAGGTGGATGCACAGGCTGAGTCGTTCATTATTTTGGACGCTTGTTTTTCCAACTAGCAAGATGGACCTTGGAAACTTTGATTAGATTTCCTCTGAAACCTATTTTCCTACTATGGATGAGTTCCGTAATTTCTCCATAAAAAGTGCTATAGGGAAAAATAGATACCCCATGAAGGTTAAGTTCTTTGTTTGGGACATTGATCTTGGTATGGAATCTCCATTAAGTTCCCAACAATTTTTCAGATGCCCTCCTTCTCTCACTTAGTTATTTAACTAATTAGTTGGCTTCCTTTTACAAATAAGAACGAACTCAACCTGTATCACTAGTAAACATACCCGAGGAAAAACTTCTAATTACAGAAATTAACATAAATACTGCTTAATTATGACTTCTACTCATCCCTTGATACAGTATTGTCCTTGGTAGTCTTCCGCCTAAGATGTGTGACATATTGGTGGTGGCTATCATAAAGATCATCCATAAGAATACCTGCATGGAAAATGCAGGTTTTTTTTTTTGTTTTGTTTTAATAAATAAAATCGAAATTTTCCCGACTATAATTGCATGGTGAAAGGACTGAGCTAGCCATTAGTGTATGTGGAACCAAATTCTAACTACTAACCGCTCTGCAAAGTCTAAGATATCGAACTCATTAAAATTCTTAGGCTGGTTTCACATCTTGTTCAAGGCCCAAGTGCCATCATGATTTCCCTCAATGTTATTCAATTCTGCATCTTGAACTTGGTGTTCTGATGGATTTATACCAAATTATTTTCGAATTTGATGCTTTGCTTACATGATTGGAGATATTGAACTCGAGCAAATGCAGTCGAGAAACTATTGATTTAATATTTTATGCACTCTTTTAGTAGACATCTCTAACCTTTTTGTTGTGTGGCTTATTTCAGAGATCATCGGTAGAGAAATAAGTTACACAGTTACTGCTGAGGTATGCTTTTTGAAACATAACCATTATCCCCATGCTTTCTAGGTCTAAGACACAGTATCCTCCCTGCAACACGTCATTGCCATTTCTGCTACATTTGTGATTTTTGCTAGGATTGTTTCTGGTTGGATTTATCTGTGGATTACGGAAAGGGCTTAAACTAAGGAATCTGTAAAATGTTCTCTAGACTAGGCAAATCAATAAACCGACCAGACCCTGGAAATTCATATTGGGTTGGAAATCTCAAGCCGTGAAAACTTTTAGTATAAGCAAAAGTTTCTATGATCTAATATCGATATGCCCCCTTTTATTTGATCATCAGACATCCAGTAAATGTTTTAATTTTGAAATCCTCATGTGACAAATTGCAGGAAATTAGAAAGCAGTTGGGAATTTCATTAATTGATGAAGAGAAAATGCAGCTATCAAAGGTAGTAAGAGAAAAGAAGTGTATGGTATTAAGATTTTGCTTGAGATATGTAAGTAGTCCACTAATATGAAGCTGTGTCATGTTTGACAGGAAAGGAGGAGATGTGAAAAATGCGGGAATGATGAAGCTTGGTTTGAGAGCAGACAGGTACACTTTGACTTTTCAAACACTAAAAATAAAACTCTAATTCCTGGACCCTTCAGGCTTTCTTTCTTTGGTTTTCCCATTAAAAAGAGTCCTAAGTTTGAGTGTGGTTCTGAATAAAAATGCAGATGAGATCGGCTGATGAAGGGCAAACCACATTTTATACCTGCACCAAGTGCCATCATCAAACCAGAGAGAATTGATCTTCCATATAAATGTTTTTAGGATGTCGCAGTGGTTCATTATAGAAATATGCATTCTGTAGCACACCATAATTCAAAGCAAAGTGCAATCATCATCCTTTGAATTATGGCTCCAATGCAATCCAAAAGCTACACGATGAATTATGATGTTCTTAGACAGTTGAAGTTAAAAATATCATGAAGTTGAAATTAATTCTTTGGATAGTGAAGGGTCTTTAGTACCTGATTTGGTAGCAA

mRNA sequence

TTTCAGTAGGCTCAGCCCAATTACATGGCATTTTCGAAAATAATGCAAAACCCTATTTTATGAGAAAAAAGAAAAACGAAAAACAAAAAGCAAAACCCTACTTTTACCTTCTCTCTCCAGGCCATTGTGATTTTTCAGACCCTTCACCTTTCTTGCCGGCTCGTCACTCTCCGCCGCTCGCTGCAGCTGGTCACTGGATCTCGCCCCGACAAGTCGTCTCTAGAAAGCTCTTGGGGAAGCAGCAAGGGCCACTCATGGCTTATTCTCGTGGACATGACTTCTTGTTTTGCAATTTGTGTGGAACCATGATGTCCTTCAGCTCAACGAAATATGTTGAGTGTCCTTTGTGCAAGTCTAGGCGAAGTGCAAAAGGGTCAGGCGAGTTGTCCCGTGAGATTAGTCAAGGTGTGCAGATCATCGGTAGAGAAATAAGTTACACAGTTACTGCTGAGGAAATTAGAAAGCAGTTGGGAATTTCATTAATTGATGAAGAGAAAATGCAGCTATCAAAGGAAAGGAGGAGATGTGAAAAATGCGGGAATGATGAAGCTTGGTTTGAGAGCAGACAGATGAGATCGGCTGATGAAGGGCAAACCACATTTTATACCTGCACCAAGTGCCATCATCAAACCAGAGAGAATTGATCTTCCATATAAATGTTTTTAGGATGTCGCAGTGGTTCATTATAGAAATATGCATTCTGTAGCACACCATAATTCAAAGCAAAGTGCAATCATCATCCTTTGAATTATGGCTCCAATGCAATCCAAAAGCTACACGATGAATTATGATGTTCTTAGACAGTTGAAGTTAAAAATATCATGAAGTTGAAATTAATTCTTTGGATAGTGAAGGGTCTTTAGTACCTGATTTGGTAGCAA

Coding sequence (CDS)

ATGAGAAAAAAGAAAAACGAAAAACAAAAAGCAAAACCCTACTTTTACCTTCTCTCTCCAGGCCATTGTGATTTTTCAGACCCTTCACCTTTCTTGCCGGCTCGTCACTCTCCGCCGCTCGCTGCAGCTGGTCACTGGATCTCGCCCCGACAAGTCGTCTCTAGAAAGCTCTTGGGGAAGCAGCAAGGGCCACTCATGGCTTATTCTCGTGGACATGACTTCTTGTTTTGCAATTTGTGTGGAACCATGATGTCCTTCAGCTCAACGAAATATGTTGAGTGTCCTTTGTGCAAGTCTAGGCGAAGTGCAAAAGGGTCAGGCGAGTTGTCCCGTGAGATTAGTCAAGGTGTGCAGATCATCGGTAGAGAAATAAGTTACACAGTTACTGCTGAGGAAATTAGAAAGCAGTTGGGAATTTCATTAATTGATGAAGAGAAAATGCAGCTATCAAAGGAAAGGAGGAGATGTGAAAAATGCGGGAATGATGAAGCTTGGTTTGAGAGCAGACAGATGAGATCGGCTGATGAAGGGCAAACCACATTTTATACCTGCACCAAGTGCCATCATCAAACCAGAGAGAATTGA

Protein sequence

MRKKKNEKQKAKPYFYLLSPGHCDFSDPSPFLPARHSPPLAAAGHWISPRQVVSRKLLGKQQGPLMAYSRGHDFLFCNLCGTMMSFSSTKYVECPLCKSRRSAKGSGELSREISQGVQIIGREISYTVTAEEIRKQLGISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN
Homology
[Close]
BLAST of Lsi08G013060 vs. ExPASy Swiss-Prot
Match: O94703 (DNA-directed RNA polymerase I subunit RPA12 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=rpa12 PE=1 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 1.9e-08
Identity = 31/120 (25.83%), Postives = 60/120 (50.00%), Query Frame = 0 

Query: 75  LFCNLCGTMMSFSSTKYVECPLCKSRRSAKGSGELSREISQGVQIIGREISYTVTAEEIR 134
           +FC+ CG ++  ++ ++  C  C+S   ++    L         ++  + S +     ++
Sbjct: 8   IFCSECGNLLESTTAQWTTCDQCQSVYPSEQFANL---------VVETKSSASAFPSALK 67

Query: 135 KQLGISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 194
            +  I  ++ +K + +    +C KCGND   F + Q+RSADEG T FY C +C ++   N
Sbjct: 68  LKHSIVQVESQKEEAATIEEKCPKCGNDHMTFHTLQLRSADEGSTVFYECPRCAYKFSTN 118

[Close]
BLAST of Lsi08G013060 vs. ExPASy Swiss-Prot
Match: Q5TM50 (DNA-directed RNA polymerase I subunit RPA12 OS=Macaca mulatta OX=9544 GN=POLR1H PE=3 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 1.6e-07
Identity = 36/123 (29.27%), Postives = 60/123 (48.78%), Query Frame = 0 

Query: 73  DFLFCNLCGTMMSFSSTK-YVECPLCKSRRSAKGSGELSREISQGVQIIGREISYTVTAE 132
           D  FC+ CG+++     +  V C  C    + +                G+ +  +V   
Sbjct: 16  DLDFCSDCGSVLPLPGAQDTVTCTRCGFNINVR-------------DFEGKVVKTSVVFH 75

Query: 133 EIRKQLGISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQT 192
           ++   + +S+ +  + Q     RRC +CG++   + +RQMRSADEGQT FYTCT C  Q 
Sbjct: 76  QLGTAMPMSVEEGPECQGPVVDRRCPRCGHEGMAYHTRQMRSADEGQTVFYTCTNCKFQE 125

Query: 193 REN 195
           +E+
Sbjct: 136 KED 125

[Close]
BLAST of Lsi08G013060 vs. ExPASy Swiss-Prot
Match: Q1RMP0 (DNA-directed RNA polymerase I subunit RPA12 OS=Bos taurus OX=9913 GN=POLR1H PE=2 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 2.1e-07
Identity = 36/123 (29.27%), Postives = 59/123 (47.97%), Query Frame = 0 

Query: 73  DFLFCNLCGTMMSFSSTK-YVECPLCKSRRSAKGSGELSREISQGVQIIGREISYTVTAE 132
           D  FC  CG+++     +  V C  C    + +                G+ +  +V   
Sbjct: 13  DLDFCPDCGSVLPLPGVQDAVACTRCGFSINVR-------------DFEGKVVKTSVVFN 72

Query: 133 EIRKQLGISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQT 192
           ++   + +S+ +  + Q     RRC +CG++   + +RQMRSADEGQT FYTCT C  Q 
Sbjct: 73  KLGTAMPLSMEEGPEFQGPVVDRRCSRCGHEGMAYHTRQMRSADEGQTVFYTCTNCKFQE 122

Query: 193 REN 195
           +E+
Sbjct: 133 KED 122

[Close]
BLAST of Lsi08G013060 vs. ExPASy Swiss-Prot
Match: Q9P1U0 (DNA-directed RNA polymerase I subunit RPA12 OS=Homo sapiens OX=9606 GN=POLR1H PE=1 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 2.8e-07
Identity = 36/123 (29.27%), Postives = 60/123 (48.78%), Query Frame = 0 

Query: 73  DFLFCNLCGTMMSFSSTK-YVECPLCKSRRSAKGSGELSREISQGVQIIGREISYTVTAE 132
           D  FC+ CG+++     +  V C  C    + +                G+ +  +V   
Sbjct: 16  DLDFCSDCGSVLPLPGAQDTVTCIRCGFNINVR-------------DFEGKVVKTSVVFH 75

Query: 133 EIRKQLGISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQT 192
           ++   + +S+ +  + Q     RRC +CG++   + +RQMRSADEGQT FYTCT C  Q 
Sbjct: 76  QLGTAMPMSVEEGPECQGPVVDRRCPRCGHEGMAYHTRQMRSADEGQTVFYTCTNCKFQE 125

Query: 193 REN 195
           +E+
Sbjct: 136 KED 125

[Close]
BLAST of Lsi08G013060 vs. ExPASy Swiss-Prot
Match: Q1XHV8 (DNA-directed RNA polymerase I subunit RPA12 OS=Pan troglodytes OX=9598 GN=POLR1H PE=3 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 2.8e-07
Identity = 36/123 (29.27%), Postives = 60/123 (48.78%), Query Frame = 0 

Query: 73  DFLFCNLCGTMMSFSSTK-YVECPLCKSRRSAKGSGELSREISQGVQIIGREISYTVTAE 132
           D  FC+ CG+++     +  V C  C    + +                G+ +  +V   
Sbjct: 16  DLDFCSDCGSVLPLPGAQDTVTCIRCGFNINVR-------------DFEGKVVKTSVVFH 75

Query: 133 EIRKQLGISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQT 192
           ++   + +S+ +  + Q     RRC +CG++   + +RQMRSADEGQT FYTCT C  Q 
Sbjct: 76  QLGTAMPMSVEEGPECQGPVVDRRCPRCGHEGMAYHTRQMRSADEGQTVFYTCTNCKFQE 125

Query: 193 REN 195
           +E+
Sbjct: 136 KED 125

[Close]
BLAST of Lsi08G013060 vs. ExPASy TrEMBL
Match: A0A6J1KC19 (DNA-directed RNA polymerase subunit OS=Cucurbita maxima OX=3661 GN=LOC111492509 PE=3 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 8.8e-57
Identity = 115/129 (89.15%), Postives = 116/129 (89.92%), Query Frame = 0 

Query: 66  MAYSRGHDFLFCNLCGTMMSFSSTKYVECPLCKSRRSAKGSGELSREISQGVQIIGREIS 125
           MAYSRGHDFLFCNLCGTM+SFSSTKYVECPLCKSRRSAKG             IIGREIS
Sbjct: 1   MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKG-------------IIGREIS 60

Query: 126 YTVTAEEIRKQLGISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCT 185
           YTVTAEEIRKQLGISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCT
Sbjct: 61  YTVTAEEIRKQLGISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCT 116

Query: 186 KCHHQTREN 195
           KCHHQTREN
Sbjct: 121 KCHHQTREN 116

[Close]
BLAST of Lsi08G013060 vs. ExPASy TrEMBL
Match: A0A6J1CTE6 (DNA-directed RNA polymerase subunit OS=Momordica charantia OX=3673 GN=LOC111014043 PE=3 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 9.7e-56
Identity = 113/129 (87.60%), Postives = 116/129 (89.92%), Query Frame = 0 

Query: 66  MAYSRGHDFLFCNLCGTMMSFSSTKYVECPLCKSRRSAKGSGELSREISQGVQIIGREIS 125
           MAYSRGHDFLFCNLCGTM+SFSSTKYVECPLCKSRRSAK             +IIGREIS
Sbjct: 1   MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAK-------------EIIGREIS 60

Query: 126 YTVTAEEIRKQLGISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCT 185
           YTVTAEEIRKQLGISLIDEEK+QLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCT
Sbjct: 61  YTVTAEEIRKQLGISLIDEEKVQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCT 116

Query: 186 KCHHQTREN 195
           KCHHQTREN
Sbjct: 121 KCHHQTREN 116

[Close]
BLAST of Lsi08G013060 vs. ExPASy TrEMBL
Match: A0A6J1HDS4 (DNA-directed RNA polymerase subunit OS=Cucurbita moschata OX=3662 GN=LOC111463020 PE=3 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 2.8e-55
Identity = 112/129 (86.82%), Postives = 115/129 (89.15%), Query Frame = 0 

Query: 66  MAYSRGHDFLFCNLCGTMMSFSSTKYVECPLCKSRRSAKGSGELSREISQGVQIIGREIS 125
           MAYS GHDFLFCNLCGTM+SFSSTKYVECPLCKSRRSAK             +I+GREIS
Sbjct: 1   MAYSGGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAK-------------EIVGREIS 60

Query: 126 YTVTAEEIRKQLGISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCT 185
           YTVTAEEIRKQLGISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCT
Sbjct: 61  YTVTAEEIRKQLGISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCT 116

Query: 186 KCHHQTREN 195
           KCHHQTREN
Sbjct: 121 KCHHQTREN 116

[Close]
BLAST of Lsi08G013060 vs. ExPASy TrEMBL
Match: A0A0A0LKC4 (DNA-directed RNA polymerase subunit OS=Cucumis sativus OX=3659 GN=Csa_2G345940 PE=3 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 1.8e-54
Identity = 110/129 (85.27%), Postives = 114/129 (88.37%), Query Frame = 0 

Query: 66  MAYSRGHDFLFCNLCGTMMSFSSTKYVECPLCKSRRSAKGSGELSREISQGVQIIGREIS 125
           MAYSRGHDFLFCNLCGTM+SF STKYVECP CKSRRSAK             +I+GREIS
Sbjct: 1   MAYSRGHDFLFCNLCGTMLSFCSTKYVECPSCKSRRSAK-------------EIVGREIS 60

Query: 126 YTVTAEEIRKQLGISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCT 185
           YTVTAEEIRKQLGISLIDEEKMQL+KERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCT
Sbjct: 61  YTVTAEEIRKQLGISLIDEEKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCT 116

Query: 186 KCHHQTREN 195
           KCHHQTREN
Sbjct: 121 KCHHQTREN 116

[Close]
BLAST of Lsi08G013060 vs. ExPASy TrEMBL
Match: A0A1S3BBZ4 (DNA-directed RNA polymerase subunit OS=Cucumis melo OX=3656 GN=LOC103488399 PE=3 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 2.6e-53
Identity = 109/129 (84.50%), Postives = 113/129 (87.60%), Query Frame = 0 

Query: 66  MAYSRGHDFLFCNLCGTMMSFSSTKYVECPLCKSRRSAKGSGELSREISQGVQIIGREIS 125
           MAYSRGHDFLFCNLCGTM+SF STKYVECPLCKS+RSAK             +I GREIS
Sbjct: 1   MAYSRGHDFLFCNLCGTMLSFCSTKYVECPLCKSKRSAK-------------EINGREIS 60

Query: 126 YTVTAEEIRKQLGISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCT 185
           YTVTAEEIRKQLGISLI EEKMQL+KERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCT
Sbjct: 61  YTVTAEEIRKQLGISLIGEEKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCT 116

Query: 186 KCHHQTREN 195
           KCHHQTREN
Sbjct: 121 KCHHQTREN 116

[Close]
BLAST of Lsi08G013060 vs. NCBI nr
Match: KAG6598510.1 (DNA-directed RNA polymerase I subunit RPA12, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 234.6 bits (597), Expect = 7.3e-58
Identity = 117/141 (82.98%), Postives = 122/141 (86.52%), Query Frame = 0 

Query: 54  SRKLLGKQQGPLMAYSRGHDFLFCNLCGTMMSFSSTKYVECPLCKSRRSAKGSGELSREI 113
           S + LG+   P MAYSRGHDFLFCNLCGTM+SFSSTKYVECPLCKSRRSAK         
Sbjct: 19  SLEALGEAARPFMAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAK--------- 78

Query: 114 SQGVQIIGREISYTVTAEEIRKQLGISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRS 173
               +I+GREISYTVTAEEIRKQLGISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRS
Sbjct: 79  ----EIVGREISYTVTAEEIRKQLGISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRS 138

Query: 174 ADEGQTTFYTCTKCHHQTREN 195
           ADEGQTTFYTCTKCHHQTREN
Sbjct: 139 ADEGQTTFYTCTKCHHQTREN 146

[Close]
BLAST of Lsi08G013060 vs. NCBI nr
Match: XP_022997634.1 (DNA-directed RNA polymerase I subunit RPA12-like [Cucurbita maxima] >XP_022997635.1 DNA-directed RNA polymerase I subunit RPA12-like [Cucurbita maxima])

HSP 1 Score: 229.9 bits (585), Expect = 1.8e-56
Identity = 115/129 (89.15%), Postives = 116/129 (89.92%), Query Frame = 0 

Query: 66  MAYSRGHDFLFCNLCGTMMSFSSTKYVECPLCKSRRSAKGSGELSREISQGVQIIGREIS 125
           MAYSRGHDFLFCNLCGTM+SFSSTKYVECPLCKSRRSAKG             IIGREIS
Sbjct: 1   MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKG-------------IIGREIS 60

Query: 126 YTVTAEEIRKQLGISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCT 185
           YTVTAEEIRKQLGISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCT
Sbjct: 61  YTVTAEEIRKQLGISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCT 116

Query: 186 KCHHQTREN 195
           KCHHQTREN
Sbjct: 121 KCHHQTREN 116

[Close]
BLAST of Lsi08G013060 vs. NCBI nr
Match: XP_023546814.1 (DNA-directed RNA polymerase I subunit RPA12-like [Cucurbita pepo subsp. pepo] >KAG7029447.1 DNA-directed RNA polymerase I subunit RPA12 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 227.6 bits (579), Expect = 9.0e-56
Identity = 113/129 (87.60%), Postives = 116/129 (89.92%), Query Frame = 0 

Query: 66  MAYSRGHDFLFCNLCGTMMSFSSTKYVECPLCKSRRSAKGSGELSREISQGVQIIGREIS 125
           MAYSRGHDFLFCNLCGTM+SFSSTKYVECPLCKSRRSAK             +I+GREIS
Sbjct: 1   MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAK-------------EIVGREIS 60

Query: 126 YTVTAEEIRKQLGISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCT 185
           YTVTAEEIRKQLGISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCT
Sbjct: 61  YTVTAEEIRKQLGISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCT 116

Query: 186 KCHHQTREN 195
           KCHHQTREN
Sbjct: 121 KCHHQTREN 116

[Close]
BLAST of Lsi08G013060 vs. NCBI nr
Match: XP_022144336.1 (DNA-directed RNA polymerase I subunit RPA12-like [Momordica charantia] >XP_022144337.1 DNA-directed RNA polymerase I subunit RPA12-like [Momordica charantia])

HSP 1 Score: 226.5 bits (576), Expect = 2.0e-55
Identity = 113/129 (87.60%), Postives = 116/129 (89.92%), Query Frame = 0 

Query: 66  MAYSRGHDFLFCNLCGTMMSFSSTKYVECPLCKSRRSAKGSGELSREISQGVQIIGREIS 125
           MAYSRGHDFLFCNLCGTM+SFSSTKYVECPLCKSRRSAK             +IIGREIS
Sbjct: 1   MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAK-------------EIIGREIS 60

Query: 126 YTVTAEEIRKQLGISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCT 185
           YTVTAEEIRKQLGISLIDEEK+QLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCT
Sbjct: 61  YTVTAEEIRKQLGISLIDEEKVQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCT 116

Query: 186 KCHHQTREN 195
           KCHHQTREN
Sbjct: 121 KCHHQTREN 116

[Close]
BLAST of Lsi08G013060 vs. NCBI nr
Match: XP_038886279.1 (DNA-directed RNA polymerase I subunit RPA12-like isoform X1 [Benincasa hispida] >XP_038886280.1 DNA-directed RNA polymerase I subunit RPA12-like isoform X1 [Benincasa hispida] >XP_038886281.1 DNA-directed RNA polymerase I subunit RPA12-like isoform X1 [Benincasa hispida] >XP_038886282.1 DNA-directed RNA polymerase I subunit RPA12-like isoform X1 [Benincasa hispida])

HSP 1 Score: 225.3 bits (573), Expect = 4.5e-55
Identity = 112/129 (86.82%), Postives = 116/129 (89.92%), Query Frame = 0 

Query: 66  MAYSRGHDFLFCNLCGTMMSFSSTKYVECPLCKSRRSAKGSGELSREISQGVQIIGREIS 125
           MAYSRGHDFLFCNLCGTM+SFSSTKYV+CPLCKS+RSAK             +IIGREIS
Sbjct: 1   MAYSRGHDFLFCNLCGTMLSFSSTKYVQCPLCKSQRSAK-------------EIIGREIS 60

Query: 126 YTVTAEEIRKQLGISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCT 185
           YTVTAEEIRKQLGISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCT
Sbjct: 61  YTVTAEEIRKQLGISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCT 116

Query: 186 KCHHQTREN 195
           KCHHQTREN
Sbjct: 121 KCHHQTREN 116

[Close]
BLAST of Lsi08G013060 vs. TAIR 10
Match: AT3G25940.1 (TFIIB zinc-binding protein )

HSP 1 Score: 123.2 bits (308), Expect = 2.2e-28
Identity = 66/131 (50.38%), Postives = 86/131 (65.65%), Query Frame = 0 

Query: 66  MAYSRGHDFLFCNLCGTMMSFSSTKYVECPLCKSRRSAKGSGELSREISQGVQIIGREIS 125
           M  SR  +FLFCNLCGTM+   STKY ECP CK+ R+AK              II +EI+
Sbjct: 1   MEKSRESEFLFCNLCGTMLVLKSTKYAECPHCKTTRNAK-------------DIIDKEIA 60

Query: 126 YTVTAEEIRKQLGISLIDEE---KMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFY 185
           YTV+AE+IR++LGISL  E+   + +L K ++ CEKC + E  + +RQ RSADEGQTT+Y
Sbjct: 61  YTVSAEDIRRELGISLFGEKTQAEAELPKIKKACEKCQHPELVYTTRQTRSADEGQTTYY 118

Query: 186 TCTKCHHQTRE 194
           TC  C H+  E
Sbjct: 121 TCPNCAHRFTE 118

[Close]
BLAST of Lsi08G013060 vs. TAIR 10
Match: AT1G20065.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; BEST Arabidopsis thaliana protein match is: TFIIB zinc-binding protein (TAIR:AT3G25940.1). )

HSP 1 Score: 74.7 bits (182), Expect = 9.1e-14
Identity = 37/75 (49.33%), Postives = 48/75 (64.00%), Query Frame = 0 

Query: 66  MAYSRGHDFLFCNLCGTMMSFSSTKYVECPLCKSRRSAKGSGELSREISQGVQIIGREIS 125
           M  SR  D LFCNLCGTM+   STKY ECPLC++ R+ K             +II + ++
Sbjct: 1   MEKSRESDILFCNLCGTMLVLKSTKYAECPLCETTRNGK-------------EIIDKNLA 60

Query: 126 YTVTAEEIRKQLGIS 141
           YTVT E+IR++LGIS
Sbjct: 61  YTVTTEDIRRELGIS 62

[Close]
BLAST of Lsi08G013060 vs. TAIR 10
Match: AT1G20065.2 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; BEST Arabidopsis thaliana protein match is: TFIIB zinc-binding protein (TAIR:AT3G25940.1). )

HSP 1 Score: 62.4 bits (150), Expect = 4.7e-10
Identity = 31/66 (46.97%), Postives = 39/66 (59.09%), Query Frame = 0 

Query: 66  MAYSRGHDFLFCNLCGTMMSFSSTKYVECPLCKSRRSAKGSGELSREISQGVQIIGREIS 125
           M  SR  D LFCNLCGTM+   STKY ECPLC++ R+ K             +II + ++
Sbjct: 1   MEKSRESDILFCNLCGTMLVLKSTKYAECPLCETTRNGK-------------EIIDKNLA 53

Query: 126 YTVTAE 132
           YTVT E
Sbjct: 61  YTVTTE 53

[Close]
BLAST of Lsi08G013060 vs. TAIR 10
Match: AT1G01210.1 (DNA-directed RNA polymerase, subunit M, archaeal )

HSP 1 Score: 48.1 bits (113), Expect = 9.1e-06
Identity = 29/102 (28.43%), Postives = 49/102 (48.04%), Query Frame = 0 

Query: 94  CPLCKS--RRSAKGSGELSREISQGVQIIGREISYTVTAEEIRKQLGISLIDEEKMQLSK 153
           CP C +  R    G+          V  I R++        ++K +   +  ++    ++
Sbjct: 4   CPTCGNLLRYEGGGNSRFFCSTCPYVAYIQRQVEIKKKQLLVKKSIEAVVTKDDIPTAAE 63

Query: 154 ERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTRE 194
               C +CG+D+A+F+S Q+RSADE ++ FY C KC    RE
Sbjct: 64  TEAPCPRCGHDKAYFKSMQIRSADEPESRFYRCLKCEFTWRE 105

[Close]
BLAST of Lsi08G013060 vs. TAIR 10
Match: AT4G07950.1 (DNA-directed RNA polymerase, subunit M, archaeal )

HSP 1 Score: 47.0 bits (110), Expect = 2.0e-05
Identity = 30/102 (29.41%), Postives = 48/102 (47.06%), Query Frame = 0 

Query: 94  CPLCKS--RRSAKGSGELSREISQGVQIIGREISYTVTAEEIRKQLGISLIDEEKMQLSK 153
           CP C +  R    GS          V  I R +        ++K +   +  ++    ++
Sbjct: 4   CPTCGNLLRYEGGGSSRFFCSTCPYVANIERRVEIKKKQLLVKKSIEPVVTKDDIPTAAE 63

Query: 154 ERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTRE 194
               C +CG+D+A+F+S Q+RSADE ++ FY C KC    RE
Sbjct: 64  TEAPCPRCGHDKAYFKSMQIRSADEPESRFYRCLKCEFTWRE 105

The following BLAST results are available for this feature:
BLAST of Lsi08G013060 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O947031.9e-0825.83DNA-directed RNA polymerase I subunit RPA12 OS=Schizosaccharomyces pombe (strain... [more]
Q5TM501.6e-0729.27DNA-directed RNA polymerase I subunit RPA12 OS=Macaca mulatta OX=9544 GN=POLR1H ... [more]
Q1RMP02.1e-0729.27DNA-directed RNA polymerase I subunit RPA12 OS=Bos taurus OX=9913 GN=POLR1H PE=2... [more]
Q9P1U02.8e-0729.27DNA-directed RNA polymerase I subunit RPA12 OS=Homo sapiens OX=9606 GN=POLR1H PE... [more]
Q1XHV82.8e-0729.27DNA-directed RNA polymerase I subunit RPA12 OS=Pan troglodytes OX=9598 GN=POLR1H... [more]
BLAST of Lsi08G013060 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1KC198.8e-5789.15DNA-directed RNA polymerase subunit OS=Cucurbita maxima OX=3661 GN=LOC111492509 ... [more]
A0A6J1CTE69.7e-5687.60DNA-directed RNA polymerase subunit OS=Momordica charantia OX=3673 GN=LOC1110140... [more]
A0A6J1HDS42.8e-5586.82DNA-directed RNA polymerase subunit OS=Cucurbita moschata OX=3662 GN=LOC11146302... [more]
A0A0A0LKC41.8e-5485.27DNA-directed RNA polymerase subunit OS=Cucumis sativus OX=3659 GN=Csa_2G345940 P... [more]
A0A1S3BBZ42.6e-5384.50DNA-directed RNA polymerase subunit OS=Cucumis melo OX=3656 GN=LOC103488399 PE=3... [more]
BLAST of Lsi08G013060 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG6598510.17.3e-5882.98DNA-directed RNA polymerase I subunit RPA12, partial [Cucurbita argyrosperma sub... [more]
XP_022997634.11.8e-5689.15DNA-directed RNA polymerase I subunit RPA12-like [Cucurbita maxima] >XP_02299763... [more]
XP_023546814.19.0e-5687.60DNA-directed RNA polymerase I subunit RPA12-like [Cucurbita pepo subsp. pepo] >K... [more]
XP_022144336.12.0e-5587.60DNA-directed RNA polymerase I subunit RPA12-like [Momordica charantia] >XP_02214... [more]
XP_038886279.14.5e-5586.82DNA-directed RNA polymerase I subunit RPA12-like isoform X1 [Benincasa hispida] ... [more]
BLAST of Lsi08G013060 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G25940.12.2e-2850.38TFIIB zinc-binding protein [more]
AT1G20065.19.1e-1449.33FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow... [more]
AT1G20065.24.7e-1046.97FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow... [more]
AT1G01210.19.1e-0628.43DNA-directed RNA polymerase, subunit M, archaeal [more]
AT4G07950.12.0e-0529.41DNA-directed RNA polymerase, subunit M, archaeal [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001222Zinc finger, TFIIS-typeSMARTSM00440Cys4_2coord: 154..193
e-value: 3.7E-12
score: 56.4
IPR001222Zinc finger, TFIIS-typePFAMPF01096TFIIS_Ccoord: 155..190
e-value: 2.2E-15
score: 56.2
IPR001222Zinc finger, TFIIS-typePROSITEPS51133ZF_TFIIS_2coord: 152..192
score: 12.826397
NoneNo IPR availableGENE3D2.20.25.10coord: 140..193
e-value: 5.9E-16
score: 59.7
NoneNo IPR availablePANTHERPTHR11239:SF14DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA12coord: 70..194
NoneNo IPR availableSUPERFAMILY57783Zinc beta-ribboncoord: 133..194
IPR012164DNA-directed RNA polymerase subunit/transcription factor SPANTHERPTHR11239DNA-DIRECTED RNA POLYMERASEcoord: 70..194
IPR034004Pol I subunit A12, C-terminal zinc ribbonCDDcd10507Zn-ribbon_RPA12coord: 154..194
e-value: 3.57202E-22
score: 82.636

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi08G013060.1Lsi08G013060.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006379 mRNA cleavage
biological_process GO:0006363 termination of RNA polymerase I transcription
biological_process GO:0006351 transcription, DNA-templated
cellular_component GO:0005736 RNA polymerase I complex
molecular_function GO:0003899 DNA-directed 5'-3' RNA polymerase activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0008270 zinc ion binding