Lsi08G010850 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi08G010850
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	carotenoid cleavage dioxygenase 8 homolog B, chloroplastic-like
Location	chr08: 19415522 .. 19419519 (-)
RNA-Seq Expression	Lsi08G010850
Synteny	Lsi08G010850

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCTCTCTCTTTCTTCCAAATTACAAACAATTTTCTCATGATGGCTTCCATGGCCACTGGAAGCTGCATTATTTCTTCCTTTAACTTCAAAACAAACCATCTAATTCTTCCCATCCACCCTTCAAAACCTACCAACTTCACCATCAGAAGCATAGCCAGTCCCATCCACCGCCGCTCATCGGTCGCCGTTCCATTGCCGGAGATTGACACCTCCGGCCTCCCGAATCACGTTGCATGGACCAGCGTTCGGCAACACCGTTGGGAAGGAGAACTCTCCGTCCAAGGCCAATTGCCCCGTTGGCTGGTTCGTTTATTATTATTATTAATGTCTTAATTATTGACAAGATTAAGATCACTTTTGTCCTGTAATATAAAATGGTAAATTTTTAAACATTAAATTTTTTTTAAATGGTTAAAGATATATTTTGACAGTAATTTTAAACGTCTGGAATGATTTTAACCATTTAAAAATTAGTATTTTAAATTAACTGTTGTGTCCTATCTTTGCATGCATATATGATAAAAATAATAGATAATAAAGAATAAAGAATTCCATTCATATATCATATGGTGGGTATTGTCGTCCAACTATAAAAATAATAATTTTATATTCAAACACTTTACAAAGTGATTAATCATTATTATTGAAATTTTGTTTTTTTTTTTGTTTTTTTTTTTTTAAAAAAATAAAGTCAATACATTATATACCCCACTTTTGATTACAATTTTACAAACTCTCCATATAATTCGACATGAATTTTTAGTCATGAAAGTTTTTCAATTTTCTAGTACAAAAAAATTATTATTATTTAACATATGCGAATGAATATATATTCTTTGTTTTAATTAATGATTTATGCCAATAGTATAAAAATATGTTCACAATTTTGTTTAAATAATAATAATAACAATAAAATAAATTTAAACAATTCTTTCAATTGTCTTTTTCGAACAGGACATAATGTTGCATGAATTAATCTTAATTATTATACCTACATGCTTAATCTTCAAATTAATCAATATTGAAAATTTCAAGGCTATTTTAACGAAAAGAAAACGAAGCCATATATTTGAAAAACTATATTAGAGTGACACTTTTCAAATTCGAGTTCAAACTTATTATTAAAAGATTGTTACATTGAATAATTTTTCGAAATGTTTTGTATAATTTCATGTGCATTTTTGCATGCAGAGTGGGACATATTTGAGAAATGGACCTGGATTATGGCATATTGGCGAATATAACTTTAGGCACCTTTTCGATGGCTACGCTACGATGGTTAAGCTTCACTTTGATGATGGTCGACTAACCGCCGGTCACCGTCAGATCGAATCGGACGCCTATAAAGCCGCCATGAAGAATCAAAAGATTTGTTATCGTGAATTTTCCGAAGTCCCCAAGTCCGATAACTTTCTTGCCTACGTCGGGGAACTTGCAAACCTCTTCTCCGGTGCATCATTGACCGATAATGCAAACACGGGCGTCGTAAAGCTCGGCGACGGTCGAGTCGTTTGCCTTACAGAGACTCAAAAGGGGTCCATAATGATCGACCCAACCACATTAGAGACTATGGGGAAGTTTGAGTATAGTGACTCGCTCGGCGGTTTGATTCACTCGGCTCATCCGATTGTTACGGACTCGGAGTTTTTGACGTTGTTGCCAGATCTTTTGAACCCGGGTTACTTGGTGGTTCGGATGGAACCAGGCTCGAATGAGCGGAAGGTGATCGGTCGAGTCAACTGCAAGGGTGGACCAGCCCCGGGTTGGGTCCATTCGTTTCCGGTGACGGAGAATTACGTGGTGGTGCCGGAGATGCCATTGAGATATTGTGCTCAAAACTTGTTGAGGGCAGAGCCGACGCCGTTGTATAAGTTTGAGTGGCGACCGGAGTCCAAAGCCTTTGTGCATGTCATGTGCAAAGCTAGTGGAACTATTGTAAGTCTTCTCTTACTTTTCTATCTTATTTTACATTATTAGAATATTGAATATCATACACAAATTAATTACACACAAGCTTATATGATGTTAATTATGCAAATAAACTTATAATAATGTATGGTTTTTAATGAAATAATGCAGGTAGCAAGTGTAGAAGTGCCATTATTCATTACATTTCATTTCATAAATGCATATGAAGAGAGGGATGAAAAAGGAAGAATTACTGCTGTGATTGCTGATTGTTGCGAACATAACGCCAACCCCGTAATCTTGGATCGACTTAGGCTTCATAATTTGCGTCGCTCTCTCAAACATCCCCTTCCGGATGCCAGGTTTGTTAAATCTCCATTTACATTTTTTTATTTTATATTTTAGGATAAATTGTAATGTAATCATGATTTGGAGAAAATTAGAATTTATAATTAAAATTTTATCCTTATAGTTTGATAAAAAAAAAAATCATAAATAGTCTACCATAGAGATTATTTACGAAAATTTTATAAAATCATATAAATTATTTATAAAGTTTTATTAAACCTGACCCATATGCAGTTAAAGTACAGTTTTTTTTTCCAATTATTATTATAATCAAAATTGGATTGTTTGTGTAATCATAAGAGTTCTTTTTTCCCCCCTTTTTATTGTGTTTATGCATGCATGCAGAAAGAGAAAAAATATATATTTTTGAAGTAAATCTAAATTCTTTCTTGCCCCAAAGTTTTTTAACAAGAAAAAGACAATGAATTCCGGAGCCGGAATTGTTGAATTTGAGAAAGAAAACAAAACAAACATTTACACAGCCGGAATTGTTGAATTCTTTTAAGAAAACCAATAACTAATCACCATAATCTGGTTTTGATTCCTTTTCTGAATGATTTGGTTTCATAAAGCTTTGACTTGTTTTGATTTAAATTCCTCACATCATGTGAAACAACGTTTTCACGTCGTTATGAGATTTTCAATAATCCTGATTCTATTACGGCCATCCATATGCCTCCTTAAATTCACAGATGTTTTAGAAGAAAGGAATGGGATGAATAATTGATAAATGGAAGTTGTGTAGGGTTGGAAGGTTCAGAATACCACTGGATGGGAGTGGATATGGGAAGCTGGAGGCAGCATTGGATCCAGATGAACATGGCAGAGGAATGGATATGTGTAGCTTCAACCCTGCTTACTTAGGCAAAAAATATAGATATGCTTATGCTTGTGGAGCCAAAAGACCCTGTAATTTCCCCAACACACTCACTAAGGTACCATTTTCCCTAATCTATGTGCTTCATCTTCGTCTTTTTTTAACTTCTTCCTCGATCCTGAATTCACTTTTGTGCTTGTTTATGAATCCGTTCGTTTCAGATTGATTTGGTGAAGAAGAAGGCCAAGAATTGGTACGAGGATGGCTCTATACCTTCTGAGCCTTTCTTCGTTGCTCGACCTGGTGCAATGGAAGAAGATGACGGTGAGTCATTTTATATCATCATCATCTAAGTGAAAAAAATGAATAAAAACACTCAACCTGAAAGGACTTGTACTTGTGATCTTCTTCACTTCTAATTAACGTATGAAATTGTATTTGTTAGTGAAAGGAAGGGAAGGGAATGTGTCTGAAATGTATTTATGTGAATGCAGGAGTGGTGATCTCTATGGTCAGTGGGAAAAATGGAGAAGGGTATGCTTTACTACTAGATGGATCAACATTTGAAGAGATAGCCAGAGCAAAATTTCCTTATGGTCTACCTTATGGCCTACATGGTTGCTGGGTTCCCAAGGATTAGGATCACTTCAATACCAAAGCATACCTTATTAAATTAATTGGTATCAGAAAATACATATATACACTATATACAGTAAGAGTAAGAAATACGTACATGTGGCGACTAACGGTGCAGCCACATAGATAGTTTGTACCTGTACAAAATGTAGGCACCTTCTTCCTCATGTATTCATCAGTTTCACTTTCAGTTTAGCCCTGTGAAAATAATATATGCAGCTTTGCTTTGTACACTATGGCCATGAATCTTTAAATTGGATTCAACAGAGGAAGTTTTCCAGAAGATGAC

mRNA sequence

CCTCTCTCTTTCTTCCAAATTACAAACAATTTTCTCATGATGGCTTCCATGGCCACTGGAAGCTGCATTATTTCTTCCTTTAACTTCAAAACAAACCATCTAATTCTTCCCATCCACCCTTCAAAACCTACCAACTTCACCATCAGAAGCATAGCCAGTCCCATCCACCGCCGCTCATCGGTCGCCGTTCCATTGCCGGAGATTGACACCTCCGGCCTCCCGAATCACGTTGCATGGACCAGCGTTCGGCAACACCGTTGGGAAGGAGAACTCTCCGTCCAAGGCCAATTGCCCCGTTGGCTGAGTGGGACATATTTGAGAAATGGACCTGGATTATGGCATATTGGCGAATATAACTTTAGGCACCTTTTCGATGGCTACGCTACGATGGTTAAGCTTCACTTTGATGATGGTCGACTAACCGCCGGTCACCGTCAGATCGAATCGGACGCCTATAAAGCCGCCATGAAGAATCAAAAGATTTGTTATCGTGAATTTTCCGAAGTCCCCAAGTCCGATAACTTTCTTGCCTACGTCGGGGAACTTGCAAACCTCTTCTCCGGTGCATCATTGACCGATAATGCAAACACGGGCGTCGTAAAGCTCGGCGACGGTCGAGTCGTTTGCCTTACAGAGACTCAAAAGGGGTCCATAATGATCGACCCAACCACATTAGAGACTATGGGGAAGTTTGAGTATAGTGACTCGCTCGGCGGTTTGATTCACTCGGCTCATCCGATTGTTACGGACTCGGAGTTTTTGACGTTGTTGCCAGATCTTTTGAACCCGGGTTACTTGGTGGTTCGGATGGAACCAGGCTCGAATGAGCGGAAGGTGATCGGTCGAGTCAACTGCAAGGGTGGACCAGCCCCGGGTTGGGTCCATTCGTTTCCGGTGACGGAGAATTACGTGGTGGTGCCGGAGATGCCATTGAGATATTGTGCTCAAAACTTGTTGAGGGCAGAGCCGACGCCGTTGTATAAGTTTGAGTGGCGACCGGAGTCCAAAGCCTTTGTGCATGTCATGTGCAAAGCTAGTGGAACTATTGTAGCAAGTGTAGAAGTGCCATTATTCATTACATTTCATTTCATAAATGCATATGAAGAGAGGGATGAAAAAGGAAGAATTACTGCTGTGATTGCTGATTGTTGCGAACATAACGCCAACCCCGTAATCTTGGATCGACTTAGGCTTCATAATTTGCGTCGCTCTCTCAAACATCCCCTTCCGGATGCCAGGTTTGTTAAATCTCCATTTACATTTTTTTATTTTATATTTTAGGATAAATTGTAATGTAATCATGATTTGGAGAAAATTAGAATTTATAATTAAAATTTTATCCTTATAGTTTGATAAAAAAAAAAATCATAAATAGTCTACCATAGAGATTATTTACGAAAATTTTATAAAATCATATAAATTATTTATAAAGTTTTATTAAACCTGACCCATATGCAGTTAAAGTACAGTTTTTTTTTCCAATTATTATTATAATCAAAATTGGATTGTTTGGTTGGAAGGTTCAGAATACCACTGGATGGGAGTGGATATGGGAAGCTGGAGGCAGCATTGGATCCAGATGAACATGGCAGAGGAATGGATATGTGTAGCTTCAACCCTGCTTACTTAGGCAAAAAATATAGATATGCTTATGCTTGTGGAGCCAAAAGACCCTGTAATTTCCCCAACACACTCACTAAGATTGATTTGGTGAAGAAGAAGGCCAAGAATTGGTACGAGGATGGCTCTATACCTTCTGAGCCTTTCTTCGTTGCTCGACCTGGTGCAATGGAAGAAGATGACGGAGTGGTGATCTCTATGGTCAGTGGGAAAAATGGAGAAGGGTATGCTTTACTACTAGATGGATCAACATTTGAAGAGATAGCCAGAGCAAAATTTCCTTATGGTCTACCTTATGGCCTACATGGTTGCTGGGTTCCCAAGGATTAGGATCACTTCAATACCAAAGCATACCTTATTAAATTAATTGGTATCAGAAAATACATATATACACTATATACAGTAAGAGTAAGAAATACGTACATGTGGCGACTAACGGTGCAGCCACATAGATAGTTTGTACCTGTACAAAATGTAGGCACCTTCTTCCTCATGTATTCATCAGTTTCACTTTCAGTTTAGCCCTGTGAAAATAATATATGCAGCTTTGCTTTGTACACTATGGCCATGAATCTTTAAATTGGATTCAACAGAGGAAGTTTTCCAGAAGATGAC

Coding sequence (CDS)

ATGATGGCTTCCATGGCCACTGGAAGCTGCATTATTTCTTCCTTTAACTTCAAAACAAACCATCTAATTCTTCCCATCCACCCTTCAAAACCTACCAACTTCACCATCAGAAGCATAGCCAGTCCCATCCACCGCCGCTCATCGGTCGCCGTTCCATTGCCGGAGATTGACACCTCCGGCCTCCCGAATCACGTTGCATGGACCAGCGTTCGGCAACACCGTTGGGAAGGAGAACTCTCCGTCCAAGGCCAATTGCCCCGTTGGCTGAGTGGGACATATTTGAGAAATGGACCTGGATTATGGCATATTGGCGAATATAACTTTAGGCACCTTTTCGATGGCTACGCTACGATGGTTAAGCTTCACTTTGATGATGGTCGACTAACCGCCGGTCACCGTCAGATCGAATCGGACGCCTATAAAGCCGCCATGAAGAATCAAAAGATTTGTTATCGTGAATTTTCCGAAGTCCCCAAGTCCGATAACTTTCTTGCCTACGTCGGGGAACTTGCAAACCTCTTCTCCGGTGCATCATTGACCGATAATGCAAACACGGGCGTCGTAAAGCTCGGCGACGGTCGAGTCGTTTGCCTTACAGAGACTCAAAAGGGGTCCATAATGATCGACCCAACCACATTAGAGACTATGGGGAAGTTTGAGTATAGTGACTCGCTCGGCGGTTTGATTCACTCGGCTCATCCGATTGTTACGGACTCGGAGTTTTTGACGTTGTTGCCAGATCTTTTGAACCCGGGTTACTTGGTGGTTCGGATGGAACCAGGCTCGAATGAGCGGAAGGTGATCGGTCGAGTCAACTGCAAGGGTGGACCAGCCCCGGGTTGGGTCCATTCGTTTCCGGTGACGGAGAATTACGTGGTGGTGCCGGAGATGCCATTGAGATATTGTGCTCAAAACTTGTTGAGGGCAGAGCCGACGCCGTTGTATAAGTTTGAGTGGCGACCGGAGTCCAAAGCCTTTGTGCATGTCATGTGCAAAGCTAGTGGAACTATTGTAGCAAGTGTAGAAGTGCCATTATTCATTACATTTCATTTCATAAATGCATATGAAGAGAGGGATGAAAAAGGAAGAATTACTGCTGTGATTGCTGATTGTTGCGAACATAACGCCAACCCCGTAATCTTGGATCGACTTAGGCTTCATAATTTGCGTCGCTCTCTCAAACATCCCCTTCCGGATGCCAGGTTTGTTAAATCTCCATTTACATTTTTTTATTTTATATTTTAG

Protein sequence

MMASMATGSCIISSFNFKTNHLILPIHPSKPTNFTIRSIASPIHRRSSVAVPLPEIDTSGLPNHVAWTSVRQHRWEGELSVQGQLPRWLSGTYLRNGPGLWHIGEYNFRHLFDGYATMVKLHFDDGRLTAGHRQIESDAYKAAMKNQKICYREFSEVPKSDNFLAYVGELANLFSGASLTDNANTGVVKLGDGRVVCLTETQKGSIMIDPTTLETMGKFEYSDSLGGLIHSAHPIVTDSEFLTLLPDLLNPGYLVVRMEPGSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPEMPLRYCAQNLLRAEPTPLYKFEWRPESKAFVHVMCKASGTIVASVEVPLFITFHFINAYEERDEKGRITAVIADCCEHNANPVILDRLRLHNLRRSLKHPLPDARFVKSPFTFFYFIF

Homology

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BLAST of Lsi08G010850 vs. ExPASy Swiss-Prot
Match: Q8LIY8 (Carotenoid cleavage dioxygenase 8 homolog B, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=CCD8B PE=1 SV=1)

HSP 1 Score: 557.4 bits (1435), Expect = 1.4e-157
Identity = 255/338 (75.44%), Postives = 295/338 (87.28%), Query Frame = 0


Query: 65  VAWTSVRQHRWEGELSVQGQLPRWLSGTYLRNGPGLWHIGEYNFRHLFDGYATMVKLHFD 124
           VAW SVRQ RWEG L V G+LP WL GTYLRNGPGLW++G+Y FRHLFDGYAT+V++ F 
Sbjct: 83  VAWKSVRQERWEGALEVDGELPLWLDGTYLRNGPGLWNLGDYGFRHLFDGYATLVRVSFR 142

Query: 125 DGRLTAGHRQIESDAYKAAMKNQKICYREFSEVPKSDNFLAYVGELANLFSGASLTDNAN 184
            GR    HRQIES+AYKAA  + K+CYREFSEVPK DNFL+YVG+LA LFSG+SLTDN+N
Sbjct: 143 GGRAVGAHRQIESEAYKAARAHGKVCYREFSEVPKPDNFLSYVGQLATLFSGSSLTDNSN 202

Query: 185 TGVVKLGDGRVVCLTETQKGSIMIDPTTLETMGKFEYSDSLGGLIHSAHPIVTDSEFLTL 244
           TGVV LGDGRV+CLTET KGSI +DP TL+T+GKF+Y+D LGGLIHSAHPIVTD+EF TL
Sbjct: 203 TGVVMLGDGRVLCLTETIKGSIQVDPDTLDTVGKFQYTDKLGGLIHSAHPIVTDTEFWTL 262

Query: 245 LPDLLNPGYLVVRMEPGSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPEMPLRYCAQ 304
           +PDL+ PGY+V RM+ GSNER+ +GRV+C+GGPAPGWVHSFPVTE+YVVVPEMPLRYCA+
Sbjct: 263 IPDLIRPGYVVARMDAGSNERQFVGRVDCRGGPAPGWVHSFPVTEHYVVVPEMPLRYCAK 322

Query: 305 NLLRAEPTPLYKFEWRPESKAFVHVMCKASGTIVASVEVPLFITFHFINAYEERDEKGRI 364
           NLLRAEPTPLYKFEW  ES +++HVMCKASG IVASVEVP F+TFHFINAYEE DE+GR+
Sbjct: 323 NLLRAEPTPLYKFEWHLESGSYMHVMCKASGKIVASVEVPPFVTFHFINAYEETDEEGRV 382

Query: 365 TAVIADCCEHNANPVILDRLRLHNLRRSL-KHPLPDAR 402
           TA+IADCCEHNAN  ILD+LRLHNLR S  +  LPDAR
Sbjct: 383 TAIIADCCEHNANTAILDKLRLHNLRSSSGQDVLPDAR 420

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BLAST of Lsi08G010850 vs. ExPASy Swiss-Prot
Match: Q8VY26 (Carotenoid cleavage dioxygenase 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CCD8 PE=1 SV=1)

HSP 1 Score: 494.2 bits (1271), Expect = 1.4e-138
Identity = 240/365 (65.75%), Postives = 288/365 (78.90%), Query Frame = 0


Query: 47  SSVAVPLPEID--TSGLPNHVAWTSVRQHRWEGELSVQGQLPRWLSGTYLRNGPGLWHIG 106
           S+   PLPE +        HVAWTSV+Q  WEGEL+VQG++P WL+GTYLRNGPGLW+IG
Sbjct: 61  SAAPSPLPEKEKVEGERRCHVAWTSVQQENWEGELTVQGKIPTWLNGTYLRNGPGLWNIG 120

Query: 107 EYNFRHLFDGYATMVKLHFDDGRLTAGHRQIESDAYKAAMKNQKICYREFSEVPKS---- 166
           +++FRHLFDGY+T+VKL FD GR+ A HR +ESDAYKAA K+ ++CYREFSE PKS    
Sbjct: 121 DHDFRHLFDGYSTLVKLQFDGGRIFAAHRLLESDAYKAAKKHNRLCYREFSETPKSVIIN 180

Query: 167 DNFLAYVGELANLFSGASLTDNANTGVVKLGDGRVVCLTETQKGSIMIDPTTLETMGKFE 226
            N  + +GE+  LFSG SLTDNANTGV+KLGDGRV+CLTETQKGSI++D  TLET+GKFE
Sbjct: 181 KNPFSGIGEIVRLFSGESLTDNANTGVIKLGDGRVMCLTETQKGSILVDHETLETIGKFE 240

Query: 227 YSDSLGG-LIHSAHPIVTDSEFLTLLPDLLNPGYLVVRMEPGSNERKVIGRVNCKGGP-A 286
           Y D L   +I SAHPIVT++E  TL+PDL+ PGY VVRME GSN+R+V+GRV C+ G   
Sbjct: 241 YDDVLSDHMIQSAHPIVTETEMWTLIPDLVKPGYRVVRMEAGSNKREVVGRVRCRSGSWG 300

Query: 287 PGWVHSFPVTENYVVVPEMPLRYCAQNLLRAEPTPLYKFEWRPESKAFVHVMCKASGTIV 346
           PGWVHSF VTENYVV+PEMPLRY  +NLLRAEPTPLYKFEW P+  AF+HVM K +G +V
Sbjct: 301 PGWVHSFAVTENYVVIPEMPLRYSVKNLLRAEPTPLYKFEWCPQDGAFIHVMSKLTGEVV 360

Query: 347 ASVEVPLFITFHFINAYEE-RDEKGRITAVIADCCEHNANPVILDRLRLHNLRRSLKHP- 402
           ASVEVP ++TFHFINAYEE ++  G+ T +IADCCEHNA+  ILD LRL  LR S  H  
Sbjct: 361 ASVEVPAYVTFHFINAYEEDKNGDGKATVIIADCCEHNADTRILDMLRLDTLRSSHGHDV 420

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BLAST of Lsi08G010850 vs. ExPASy Swiss-Prot
Match: Q93VD5 (Carotenoid cleavage dioxygenase 8 homolog A, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=CCD8A PE=2 SV=1)

HSP 1 Score: 423.7 bits (1088), Expect = 2.4e-117
Identity = 203/341 (59.53%), Postives = 257/341 (75.37%), Query Frame = 0


Query: 66  AWTSVRQHRWEGELSVQGQLPRWLSGTYLRNGPGLWHIGEYNFRHLFDGYATMVKLHFDD 125
           A TSVRQ RWEG+L ++G LP WL+GTY+RNGPG+W +GE+ F HLFDGYAT+V++ F  
Sbjct: 68  ASTSVRQERWEGDLPIEGCLPPWLNGTYIRNGPGMWDVGEHAFHHLFDGYATLVRVSFRG 127

Query: 126 G---RLTAGHRQIESDAYKAAMKNQKICYREFSEVP-KSDNFLAYVGELANLFSGASLTD 185
           G   R T  HRQIES+AY+AA+   +   REFS  P  + + L   G+L  L +GA+LTD
Sbjct: 128 GGGARATGAHRQIESEAYRAAVARGRPVLREFSHCPAPAKSLLHRFGDLVGLVTGAALTD 187

Query: 186 NANTGVVKLGDGRVVCLTETQKGSIMIDPTTLETMGKFEYSDSLGGLIHSAHPIVTDSEF 245
           N N+ V+ LGDGRV+CLTET K S++IDP TLET+G+F Y+D LGG++ SAHPIVTD+EF
Sbjct: 188 NPNSAVLPLGDGRVMCLTETTKSSVLIDPDTLETVGRFRYTDRLGGMVQSAHPIVTDTEF 247

Query: 246 LTLLPDLLNPGYLVVRMEPGSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPEMPLRY 305
           LTLLPDL+ PG+LVVRME GSNERKVIGR++C+GGP+PGW+HSF VTE Y VVPEMPLRY
Sbjct: 248 LTLLPDLVRPGHLVVRMEAGSNERKVIGRMDCRGGPSPGWLHSFAVTEKYAVVPEMPLRY 307

Query: 306 CAQNLLRAEPTPLYKFEWRPESKAFVHVMCKASGTIVASVEVPLFITFHFINAYEERDEK 365
            + +LL +E  P Y F+W P S +++HVMCK++G  VASVEVP F+  HFINAYE   E+
Sbjct: 308 SSASLLASELAPFYAFDWVPASGSYMHVMCKSTGKTVASVEVPPFMAIHFINAYE---EE 367

Query: 366 GRITAVIADCCEHNANPVILDRLRLHNLRRSL-KHPLPDAR 402
           G   AV+ DCCEH  +P I++ L L  LR    K  LP+AR
Sbjct: 368 GDEAAVVVDCCEHYGDPAIIETLVLSRLRLLRGKDVLPNAR 405

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BLAST of Lsi08G010850 vs. ExPASy Swiss-Prot
Match: Q9VFS2 (Carotenoid isomerooxygenase OS=Drosophila melanogaster OX=7227 GN=ninaB PE=1 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 8.4e-30
Identity = 89/336 (26.49%), Postives = 152/336 (45.24%), Query Frame = 0


Query: 83  GQLPRWLSGTYLRNGPGLWHIGEYNFRHLFDGYATMVKLHFDDGRLTAGHRQIESDAYKA 142
           G +P+W+ G+ LRNGPG W +G+  F HLFD  A + +    +GR+T  +R ++++  + 
Sbjct: 63  GHIPKWICGSLLRNGPGSWKVGDMTFGHLFDCSALLHRFAIRNGRVTYQNRFVDTETLRK 122

Query: 143 AMKNQKICYREFSEVPKSDNFLAYVGELANLFSGASLTDNANTGVVKLGDGRVVCLTETQ 202
               Q+I   EF      D   +     A +F   S TDN+   +   GD +    TET 
Sbjct: 123 NRSAQRIVVTEFGTAAVPDPCHSIFDRFAAIFRPDSGTDNSMISIYPFGD-QYYTFTET- 182

Query: 203 KGSIMIDPTTLETMGKFEYSDSLGGLIHSAHPIVTDSEFLTLLPDLL---NPGYLVVRME 262
                I+P TL T  +   +D +G + H++HP V  S  +  L   +    P Y ++   
Sbjct: 183 PFMHRINPCTLATEARICTTDFVGVVNHTSHPHVLPSGTVYNLGTTMTRSGPAYTILSFP 242

Query: 263 PGS---NERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPEMPLRYCAQNLLRAE---PTP 322
            G     +  V+  + C+    PG++H+F +T++Y V+ E PL       ++A+      
Sbjct: 243 HGEQMFEDAHVVATLPCRWKLHPGYMHTFGLTDHYFVIVEQPLSVSLTEYIKAQLGGQNL 302

Query: 323 LYKFEWRPESKAFVHVMCKASGTIVASVEVPLFITFHFINAYEERDEKGRITAVIADCCE 382
               +W  +     H++ + SG +V + E   F   H IN +E      R   V+ D C 
Sbjct: 303 SACLKWFEDRPTLFHLIDRVSGKLVQTYESEAFFYLHIINCFE------RDGHVVVDICS 362

Query: 383 HNANPVILDRLRLHNLRRSLKHPLPDARFVKSPFTF 410
           +  NP +++ + L  +     +P     F   P  F
Sbjct: 363 YR-NPEMINCMYLEAIANMQTNPNYATLFRGRPLRF 389

[Close]

BLAST of Lsi08G010850 vs. ExPASy Swiss-Prot
Match: A9C3R8 (Retinal Mueller cells isomerohydrolase OS=Danio rerio OX=7955 GN=rpe65c PE=1 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 1.1e-29
Identity = 99/348 (28.45%), Postives = 155/348 (44.54%), Query Frame = 0


Query: 81  VQGQLPRWLSGTYLRNGPGLWHIGEYNFRHLFDGYATMVKLHFDDGRLTAGHRQIESDAY 140
           V G++P WLSG+ LR GPGL+ +G+  F HLFDG A + K    DGR+T   R I +DAY
Sbjct: 30  VSGEIPAWLSGSLLRMGPGLFEVGDEPFYHLFDGQALLHKFDLKDGRVTYHRRFIRTDAY 89

Query: 141 KAAMKNQKICYREFSEVPKSDNFLAYVGELANLFSGASLTDNANTGVVKLGDGRVVCLTE 200
             AM  +++   EF      D            F G  +TDN    +  +G+    C TE
Sbjct: 90  VRAMTEKRVVITEFGTTAYPDPCKNIFSRFFTYFQGIEVTDNCLVNIYPIGEDFYAC-TE 149

Query: 201 TQKGSIMIDPTTLETMGKFEYSDSLGGLIHSAHP-IVTDSEFLTL---LPDLLNPGYLVV 260
           T      +DP TLET+ K +  + L     +AHP I  D     +       ++  Y +V
Sbjct: 150 T-NFITKVDPDTLETVKKVDLCNYLSVNGLTAHPHIEADGTVYNIGNCFGKNMSLAYNIV 209

Query: 261 RMEPGSNER-------KVIGRVNCKGGPAPGWVHSFPVTENYVVVPEMPLRYCAQNLLRA 320
           ++ P   ++       K++ +        P +VHSF +TEN+ V  E P++    NLL+ 
Sbjct: 210 KIPPLQEDKSDQFEKSKILVQFPSSERFKPSYVHSFGITENHFVFVETPVKI---NLLKF 269

Query: 321 EPTPLYK-------FEWRPESKAFVHVMCKASGTIV-ASVEVPLFITFHFINAYEERDEK 380
             +   +       FE   +   + H+  K  G  +        F  FH IN +E++   
Sbjct: 270 LTSWSIRGSNYMDCFESNDKMGTWFHLAAKNPGKYIDHKFRTSAFNIFHHINCFEDQG-- 329

Query: 381 GRITAVIADCCEHNANPVILDRLRLHNLRR--------SLKHPLPDAR 402
                ++ D C    +  + + L L NLR+        +L+ P P+ R
Sbjct: 330 ----FIVVDLCTWKGHEFVYNYLYLANLRQNWEEVKKAALRAPQPEVR 366

[Close]

BLAST of Lsi08G010850 vs. ExPASy TrEMBL
Match: A0A0A0LS17 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G373590 PE=3 SV=1)

HSP 1 Score: 752.3 bits (1941), Expect = 1.1e-213
Identity = 366/404 (90.59%), Postives = 380/404 (94.06%), Query Frame = 0


Query: 1   MMASMATGSCIISSFNFKTNHLILPIHP-SKPT--NFTIRSIASPIHRRSSVAVPLPEID 60
           MMASMA GSCIIS FNFKT H ILPI P SKPT  NFTIRSIASP+H  S V VPLPEID
Sbjct: 1   MMASMAMGSCIISPFNFKTKHPILPIQPSSKPTLSNFTIRSIASPVHPPSPVVVPLPEID 60

Query: 61  TSGLPNHVAWTSVRQHRWEGELSVQGQLPRWLSGTYLRNGPGLWHIGEYNFRHLFDGYAT 120
           TS   NHVAWTS+RQ RWEGELS+QG LP WLSGTYLRNGPGLW+IG+YNFRHLFDGYAT
Sbjct: 61  TSN--NHVAWTSIRQDRWEGELSIQGHLPSWLSGTYLRNGPGLWNIGDYNFRHLFDGYAT 120

Query: 121 MVKLHFDDGRLTAGHRQIESDAYKAAMKNQKICYREFSEVPKSDNFLAYVGELANLFSGA 180
           +VKLHFD+GRL AGHRQIES+AYKAAMKNQKICYREFSEVPK+DNFLAYVGELANLFSGA
Sbjct: 121 IVKLHFDNGRLIAGHRQIESNAYKAAMKNQKICYREFSEVPKADNFLAYVGELANLFSGA 180

Query: 181 SLTDNANTGVVKLGDGRVVCLTETQKGSIMIDPTTLETMGKFEYSDSLGGLIHSAHPIVT 240
           SLTDNANTGVVKLGDGRVVCLTETQKGSIMIDP TLET+G+FEYSDSLGGLIHSAHPIVT
Sbjct: 181 SLTDNANTGVVKLGDGRVVCLTETQKGSIMIDPDTLETVGQFEYSDSLGGLIHSAHPIVT 240

Query: 241 DSEFLTLLPDLLNPGYLVVRMEPGSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPEM 300
           DSEFLTLLPDLLNPGYLVVRMEP SNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPEM
Sbjct: 241 DSEFLTLLPDLLNPGYLVVRMEPSSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPEM 300

Query: 301 PLRYCAQNLLRAEPTPLYKFEWRPESKAFVHVMCKASGTIVASVEVPLFITFHFINAYEE 360
           PLRYCAQNLLRAEPTPLYKFEWRPESKAF+HVMCKASG IVASVEVPLFITFHFINAYEE
Sbjct: 301 PLRYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGNIVASVEVPLFITFHFINAYEE 360

Query: 361 RDEKGRITAVIADCCEHNANPVILDRLRLHNLRRSLKHPLPDAR 402
           RDEKGRITAVIADCCEHNA+P ILDRLRLHNLR SLK+PLPDAR
Sbjct: 361 RDEKGRITAVIADCCEHNADPAILDRLRLHNLRDSLKYPLPDAR 402

[Close]

BLAST of Lsi08G010850 vs. ExPASy TrEMBL
Match: A0A5A7VHT5 (Carotenoid cleavage dioxygenase 8-like protein B OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G003670 PE=3 SV=1)

HSP 1 Score: 749.6 bits (1934), Expect = 7.0e-213
Identity = 364/405 (89.88%), Postives = 378/405 (93.33%), Query Frame = 0


Query: 1   MMASMATGSCIISSFNFKTNHLILPIH--PSKPT--NFTIRSIASPIHRRSSVAVPLPEI 60
           MMASMA GSCIIS FNFKT H +LPI    SKPT  NFTIRSIASP+   S V VPLPEI
Sbjct: 1   MMASMAIGSCIISPFNFKTKHPLLPIQQPSSKPTLSNFTIRSIASPVRPPSPVVVPLPEI 60

Query: 61  DTSGLPNHVAWTSVRQHRWEGELSVQGQLPRWLSGTYLRNGPGLWHIGEYNFRHLFDGYA 120
           DT   PNHVAWTS+RQ RWEGELSVQG LP WLSGTYLRNGPGLW+IG+Y+FRHLFDGYA
Sbjct: 61  DTFDAPNHVAWTSIRQDRWEGELSVQGHLPSWLSGTYLRNGPGLWNIGDYDFRHLFDGYA 120

Query: 121 TMVKLHFDDGRLTAGHRQIESDAYKAAMKNQKICYREFSEVPKSDNFLAYVGELANLFSG 180
            +VKLHFD+GRL AGHRQIES+AYKAAMKNQKICYREFSEVPK+DNFLAYVGELANLFSG
Sbjct: 121 MIVKLHFDNGRLIAGHRQIESNAYKAAMKNQKICYREFSEVPKADNFLAYVGELANLFSG 180

Query: 181 ASLTDNANTGVVKLGDGRVVCLTETQKGSIMIDPTTLETMGKFEYSDSLGGLIHSAHPIV 240
           ASLTDNANTGVVKLGDGRVVCLTETQKGSIMIDP TLET+GKFEYSDSLGGLIHSAHPIV
Sbjct: 181 ASLTDNANTGVVKLGDGRVVCLTETQKGSIMIDPDTLETVGKFEYSDSLGGLIHSAHPIV 240

Query: 241 TDSEFLTLLPDLLNPGYLVVRMEPGSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPE 300
           TDSEFLTLLPDLLNPGYLVVRMEP SNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPE
Sbjct: 241 TDSEFLTLLPDLLNPGYLVVRMEPSSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPE 300

Query: 301 MPLRYCAQNLLRAEPTPLYKFEWRPESKAFVHVMCKASGTIVASVEVPLFITFHFINAYE 360
           MPLRYCAQNLL+AEPTPLYKFEWRPESKAF+HVMCKASG IVASVEVPLFITFHFINAYE
Sbjct: 301 MPLRYCAQNLLKAEPTPLYKFEWRPESKAFMHVMCKASGNIVASVEVPLFITFHFINAYE 360

Query: 361 ERDEKGRITAVIADCCEHNANPVILDRLRLHNLRRSLKHPLPDAR 402
           ERDEKGRITAVIADCCEHNANPVILDRLRLHNLR SLK+PLPDAR
Sbjct: 361 ERDEKGRITAVIADCCEHNANPVILDRLRLHNLRDSLKYPLPDAR 405

[Close]

BLAST of Lsi08G010850 vs. ExPASy TrEMBL
Match: A0A1S3BBP5 (carotenoid cleavage dioxygenase 8 homolog B, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=CCD8 PE=3 SV=1)

HSP 1 Score: 749.6 bits (1934), Expect = 7.0e-213
Identity = 364/405 (89.88%), Postives = 378/405 (93.33%), Query Frame = 0


Query: 1   MMASMATGSCIISSFNFKTNHLILPIH--PSKPT--NFTIRSIASPIHRRSSVAVPLPEI 60
           MMASMA GSCIIS FNFKT H +LPI    SKPT  NFTIRSIASP+   S V VPLPEI
Sbjct: 1   MMASMAIGSCIISPFNFKTKHPLLPIQQPSSKPTLSNFTIRSIASPVRPPSPVVVPLPEI 60

Query: 61  DTSGLPNHVAWTSVRQHRWEGELSVQGQLPRWLSGTYLRNGPGLWHIGEYNFRHLFDGYA 120
           DT   PNHVAWTS+RQ RWEGELSVQG LP WLSGTYLRNGPGLW+IG+Y+FRHLFDGYA
Sbjct: 61  DTFDAPNHVAWTSIRQDRWEGELSVQGHLPSWLSGTYLRNGPGLWNIGDYDFRHLFDGYA 120

Query: 121 TMVKLHFDDGRLTAGHRQIESDAYKAAMKNQKICYREFSEVPKSDNFLAYVGELANLFSG 180
            +VKLHFD+GRL AGHRQIES+AYKAAMKNQKICYREFSEVPK+DNFLAYVGELANLFSG
Sbjct: 121 MIVKLHFDNGRLIAGHRQIESNAYKAAMKNQKICYREFSEVPKADNFLAYVGELANLFSG 180

Query: 181 ASLTDNANTGVVKLGDGRVVCLTETQKGSIMIDPTTLETMGKFEYSDSLGGLIHSAHPIV 240
           ASLTDNANTGVVKLGDGRVVCLTETQKGSIMIDP TLET+GKFEYSDSLGGLIHSAHPIV
Sbjct: 181 ASLTDNANTGVVKLGDGRVVCLTETQKGSIMIDPDTLETVGKFEYSDSLGGLIHSAHPIV 240

Query: 241 TDSEFLTLLPDLLNPGYLVVRMEPGSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPE 300
           TDSEFLTLLPDLLNPGYLVVRMEP SNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPE
Sbjct: 241 TDSEFLTLLPDLLNPGYLVVRMEPSSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPE 300

Query: 301 MPLRYCAQNLLRAEPTPLYKFEWRPESKAFVHVMCKASGTIVASVEVPLFITFHFINAYE 360
           MPLRYCAQNLL+AEPTPLYKFEWRPESKAF+HVMCKASG IVASVEVPLFITFHFINAYE
Sbjct: 301 MPLRYCAQNLLKAEPTPLYKFEWRPESKAFMHVMCKASGNIVASVEVPLFITFHFINAYE 360

Query: 361 ERDEKGRITAVIADCCEHNANPVILDRLRLHNLRRSLKHPLPDAR 402
           ERDEKGRITAVIADCCEHNANPVILDRLRLHNLR SLK+PLPDAR
Sbjct: 361 ERDEKGRITAVIADCCEHNANPVILDRLRLHNLRDSLKYPLPDAR 405

[Close]

BLAST of Lsi08G010850 vs. ExPASy TrEMBL
Match: A0A023VSV7 (Carotenoid cleavage dioxygenase 8 OS=Cucumis melo OX=3656 GN=CCD8 PE=2 SV=1)

HSP 1 Score: 746.9 bits (1927), Expect = 4.5e-212
Identity = 365/406 (89.90%), Postives = 378/406 (93.10%), Query Frame = 0


Query: 1   MMASMATGSCIISSFNFKTNHLILPIH--PSKPT--NFTIRSIASPIHRRSSVAVPLPEI 60
           MMASMA GSCIIS FNFKT H +LPI    SKPT  NFTIRSIASP+   S V VPLPEI
Sbjct: 1   MMASMAIGSCIISPFNFKTKHPLLPIQQPSSKPTLSNFTIRSIASPVRPPSPVVVPLPEI 60

Query: 61  DTSGLPNHVAWTSVRQHRWEGELSVQGQLPRWL-SGTYLRNGPGLWHIGEYNFRHLFDGY 120
           DT   PNHVAWTS+RQ RWEGELSVQG LP WL SGTYLRNGPGLW+IG+YNFRHLFDGY
Sbjct: 61  DTFDAPNHVAWTSIRQDRWEGELSVQGHLPSWLVSGTYLRNGPGLWNIGDYNFRHLFDGY 120

Query: 121 ATMVKLHFDDGRLTAGHRQIESDAYKAAMKNQKICYREFSEVPKSDNFLAYVGELANLFS 180
           A +VKLHFD+GRL AGHRQIES+AYKAAMKNQKICYREFSEVPK+DNFLAYVGELANLFS
Sbjct: 121 AMIVKLHFDNGRLIAGHRQIESNAYKAAMKNQKICYREFSEVPKADNFLAYVGELANLFS 180

Query: 181 GASLTDNANTGVVKLGDGRVVCLTETQKGSIMIDPTTLETMGKFEYSDSLGGLIHSAHPI 240
           GASLTDNANTGVVKLGDGRVVCLTETQKGSIMIDP TLET+GKFEYSDSLGGLIHSAHPI
Sbjct: 181 GASLTDNANTGVVKLGDGRVVCLTETQKGSIMIDPDTLETVGKFEYSDSLGGLIHSAHPI 240

Query: 241 VTDSEFLTLLPDLLNPGYLVVRMEPGSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVP 300
           VTDSEFLTLLPDLLNPGYLVVRMEP SNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVP
Sbjct: 241 VTDSEFLTLLPDLLNPGYLVVRMEPSSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVP 300

Query: 301 EMPLRYCAQNLLRAEPTPLYKFEWRPESKAFVHVMCKASGTIVASVEVPLFITFHFINAY 360
           EMPLRYCAQNLL+AEPTPLYKFEWRPESKAF+HVMCKASG IVASVEVPLFITFHFINAY
Sbjct: 301 EMPLRYCAQNLLKAEPTPLYKFEWRPESKAFMHVMCKASGNIVASVEVPLFITFHFINAY 360

Query: 361 EERDEKGRITAVIADCCEHNANPVILDRLRLHNLRRSLKHPLPDAR 402
           EERDEKGRITAVIADCCEHNANPVILDRLRLHNLR SLK+PLPDAR
Sbjct: 361 EERDEKGRITAVIADCCEHNANPVILDRLRLHNLRDSLKYPLPDAR 406

[Close]

BLAST of Lsi08G010850 vs. ExPASy TrEMBL
Match: A0A6J1HGQ6 (carotenoid cleavage dioxygenase 8 homolog B, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111462801 PE=3 SV=1)

HSP 1 Score: 723.4 bits (1866), Expect = 5.4e-205
Identity = 351/403 (87.10%), Postives = 373/403 (92.56%), Query Frame = 0


Query: 2   MASMATGSCIISSFNFKTNHLILPIHPSKPT--NFTIRSIASPIHRRSSVAVPLPEIDTS 61
           MASMA GSCI    +FK  +  LP+HP K T  +FTIRSIASPI     V VPLP+IDTS
Sbjct: 1   MASMAAGSCI----SFKPYNPCLPLHPPKRTVSSFTIRSIASPIRPSPPVVVPLPDIDTS 60

Query: 62  GLPNHVAWTSVRQHRWEGELSVQGQLPRWLSGTYLRNGPGLWHIGEYNFRHLFDGYATMV 121
             P HVAWTS+RQ RWEG+LSVQGQLPRWL+GTYLRNGPG+W+IG YNFRHLFDGYAT+V
Sbjct: 61  IPPTHVAWTSIRQDRWEGQLSVQGQLPRWLNGTYLRNGPGVWNIGNYNFRHLFDGYATIV 120

Query: 122 KLHFDDGRLTAGHRQIESDAYKAAMKNQKICYREFSEVPKSDNFLAYVGELANLFSGASL 181
           KLHF+DGRL AGHRQIES+AYKAAMKNQK+CYREFSEVPK+DNFLAYVGELANLFSG SL
Sbjct: 121 KLHFEDGRLIAGHRQIESNAYKAAMKNQKVCYREFSEVPKTDNFLAYVGELANLFSGVSL 180

Query: 182 TDNANTGVVKLGDGRVVCLTETQKGSIMIDPTTLETMGKFEYSDSLGGLIHSAHPIVTDS 241
           TDNANTGVVKLGD RVVCLTETQKGSI+I+P+TLET+GKFEYSDSLGGLIHSAHPIVTD 
Sbjct: 181 TDNANTGVVKLGDSRVVCLTETQKGSIIINPSTLETIGKFEYSDSLGGLIHSAHPIVTDL 240

Query: 242 EFLTLLPDLLNPGYLVVRMEPGSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPEMPL 301
           EFLTLLPDLLNPGYLVVRMEPGSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPEMPL
Sbjct: 241 EFLTLLPDLLNPGYLVVRMEPGSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPEMPL 300

Query: 302 RYCAQNLLRAEPTPLYKFEWRPESKAFVHVMCKASGTIVASVEVPLFITFHFINAYEERD 361
           RYCAQNLLRAEPTPLYKFEWRPESKAF+HVMCKASG+IVASVEVPLFITFHFINAYEERD
Sbjct: 301 RYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGSIVASVEVPLFITFHFINAYEERD 360

Query: 362 EKGRITAVIADCCEHNANPVILDRLRLHNLRR-SLKHPLPDAR 402
           EKGRITAVIADCCEHNANP+ILDRLRLHNLRR SLKH LPDAR
Sbjct: 361 EKGRITAVIADCCEHNANPLILDRLRLHNLRRFSLKHALPDAR 399

[Close]

BLAST of Lsi08G010850 vs. NCBI nr
Match: XP_038885421.1 (carotenoid cleavage dioxygenase 8 homolog B, chloroplastic [Benincasa hispida])

HSP 1 Score: 770.0 bits (1987), Expect = 1.0e-218
Identity = 373/403 (92.56%), Postives = 384/403 (95.29%), Query Frame = 0


Query: 1   MMASMATGSCIISSFNFKTNHLILPIHPSKPT--NFTIRSIASPIHRRSSVAVPLPEIDT 60
           MMASMA GS IIS FNFKTNH     HPSKPT  NFTIRSIASPI R S + VPLPEIDT
Sbjct: 1   MMASMAIGSSIISPFNFKTNH-----HPSKPTLSNFTIRSIASPIRRPSPIVVPLPEIDT 60

Query: 61  SGLPNHVAWTSVRQHRWEGELSVQGQLPRWLSGTYLRNGPGLWHIGEYNFRHLFDGYATM 120
           +G PNHVAWTSVRQ RWEGELSV+GQLPRWLSGTYLRNGPGLW+IG+YNFRHLFDGYAT+
Sbjct: 61  TGPPNHVAWTSVRQDRWEGELSVRGQLPRWLSGTYLRNGPGLWNIGDYNFRHLFDGYATI 120

Query: 121 VKLHFDDGRLTAGHRQIESDAYKAAMKNQKICYREFSEVPKSDNFLAYVGELANLFSGAS 180
           VKLHFDDGRL AGHRQIESDAYKAAMKNQKICYREFSEVPK+DNFLAYVGELANLFSGAS
Sbjct: 121 VKLHFDDGRLIAGHRQIESDAYKAAMKNQKICYREFSEVPKADNFLAYVGELANLFSGAS 180

Query: 181 LTDNANTGVVKLGDGRVVCLTETQKGSIMIDPTTLETMGKFEYSDSLGGLIHSAHPIVTD 240
           LTDNANTGV+KLGDGRVVCLTETQKGSIMIDPTTLET+GKFEYSDSLGGLIHSAHPIVTD
Sbjct: 181 LTDNANTGVIKLGDGRVVCLTETQKGSIMIDPTTLETVGKFEYSDSLGGLIHSAHPIVTD 240

Query: 241 SEFLTLLPDLLNPGYLVVRMEPGSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPEMP 300
           +EFLTLLPDLLNPGYLVVRMEPGSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPEMP
Sbjct: 241 TEFLTLLPDLLNPGYLVVRMEPGSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPEMP 300

Query: 301 LRYCAQNLLRAEPTPLYKFEWRPESKAFVHVMCKASGTIVASVEVPLFITFHFINAYEER 360
           LRYCAQNLLRAEPTPLYKFEWRPESKAFVHVMCKASG IVASVEVPLFITFHFINAYEER
Sbjct: 301 LRYCAQNLLRAEPTPLYKFEWRPESKAFVHVMCKASGNIVASVEVPLFITFHFINAYEER 360

Query: 361 DEKGRITAVIADCCEHNANPVILDRLRLHNLRRSLKHPLPDAR 402
           DEKGRITAVIADCCEHNANPVILDRLRLHNLRRSLK+ LPDAR
Sbjct: 361 DEKGRITAVIADCCEHNANPVILDRLRLHNLRRSLKYALPDAR 398

[Close]

BLAST of Lsi08G010850 vs. NCBI nr
Match: XP_004148387.1 (carotenoid cleavage dioxygenase 8 homolog B, chloroplastic [Cucumis sativus] >KGN62816.1 hypothetical protein Csa_022620 [Cucumis sativus])

HSP 1 Score: 752.3 bits (1941), Expect = 2.2e-213
Identity = 366/404 (90.59%), Postives = 380/404 (94.06%), Query Frame = 0


Query: 1   MMASMATGSCIISSFNFKTNHLILPIHP-SKPT--NFTIRSIASPIHRRSSVAVPLPEID 60
           MMASMA GSCIIS FNFKT H ILPI P SKPT  NFTIRSIASP+H  S V VPLPEID
Sbjct: 1   MMASMAMGSCIISPFNFKTKHPILPIQPSSKPTLSNFTIRSIASPVHPPSPVVVPLPEID 60

Query: 61  TSGLPNHVAWTSVRQHRWEGELSVQGQLPRWLSGTYLRNGPGLWHIGEYNFRHLFDGYAT 120
           TS   NHVAWTS+RQ RWEGELS+QG LP WLSGTYLRNGPGLW+IG+YNFRHLFDGYAT
Sbjct: 61  TSN--NHVAWTSIRQDRWEGELSIQGHLPSWLSGTYLRNGPGLWNIGDYNFRHLFDGYAT 120

Query: 121 MVKLHFDDGRLTAGHRQIESDAYKAAMKNQKICYREFSEVPKSDNFLAYVGELANLFSGA 180
           +VKLHFD+GRL AGHRQIES+AYKAAMKNQKICYREFSEVPK+DNFLAYVGELANLFSGA
Sbjct: 121 IVKLHFDNGRLIAGHRQIESNAYKAAMKNQKICYREFSEVPKADNFLAYVGELANLFSGA 180

Query: 181 SLTDNANTGVVKLGDGRVVCLTETQKGSIMIDPTTLETMGKFEYSDSLGGLIHSAHPIVT 240
           SLTDNANTGVVKLGDGRVVCLTETQKGSIMIDP TLET+G+FEYSDSLGGLIHSAHPIVT
Sbjct: 181 SLTDNANTGVVKLGDGRVVCLTETQKGSIMIDPDTLETVGQFEYSDSLGGLIHSAHPIVT 240

Query: 241 DSEFLTLLPDLLNPGYLVVRMEPGSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPEM 300
           DSEFLTLLPDLLNPGYLVVRMEP SNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPEM
Sbjct: 241 DSEFLTLLPDLLNPGYLVVRMEPSSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPEM 300

Query: 301 PLRYCAQNLLRAEPTPLYKFEWRPESKAFVHVMCKASGTIVASVEVPLFITFHFINAYEE 360
           PLRYCAQNLLRAEPTPLYKFEWRPESKAF+HVMCKASG IVASVEVPLFITFHFINAYEE
Sbjct: 301 PLRYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGNIVASVEVPLFITFHFINAYEE 360

Query: 361 RDEKGRITAVIADCCEHNANPVILDRLRLHNLRRSLKHPLPDAR 402
           RDEKGRITAVIADCCEHNA+P ILDRLRLHNLR SLK+PLPDAR
Sbjct: 361 RDEKGRITAVIADCCEHNADPAILDRLRLHNLRDSLKYPLPDAR 402

[Close]

BLAST of Lsi08G010850 vs. NCBI nr
Match: XP_008445014.1 (PREDICTED: carotenoid cleavage dioxygenase 8 homolog B, chloroplastic isoform X1 [Cucumis melo] >KAA0065039.1 carotenoid cleavage dioxygenase 8-like protein B [Cucumis melo var. makuwa])

HSP 1 Score: 749.6 bits (1934), Expect = 1.4e-212
Identity = 364/405 (89.88%), Postives = 378/405 (93.33%), Query Frame = 0


Query: 1   MMASMATGSCIISSFNFKTNHLILPIH--PSKPT--NFTIRSIASPIHRRSSVAVPLPEI 60
           MMASMA GSCIIS FNFKT H +LPI    SKPT  NFTIRSIASP+   S V VPLPEI
Sbjct: 1   MMASMAIGSCIISPFNFKTKHPLLPIQQPSSKPTLSNFTIRSIASPVRPPSPVVVPLPEI 60

Query: 61  DTSGLPNHVAWTSVRQHRWEGELSVQGQLPRWLSGTYLRNGPGLWHIGEYNFRHLFDGYA 120
           DT   PNHVAWTS+RQ RWEGELSVQG LP WLSGTYLRNGPGLW+IG+Y+FRHLFDGYA
Sbjct: 61  DTFDAPNHVAWTSIRQDRWEGELSVQGHLPSWLSGTYLRNGPGLWNIGDYDFRHLFDGYA 120

Query: 121 TMVKLHFDDGRLTAGHRQIESDAYKAAMKNQKICYREFSEVPKSDNFLAYVGELANLFSG 180
            +VKLHFD+GRL AGHRQIES+AYKAAMKNQKICYREFSEVPK+DNFLAYVGELANLFSG
Sbjct: 121 MIVKLHFDNGRLIAGHRQIESNAYKAAMKNQKICYREFSEVPKADNFLAYVGELANLFSG 180

Query: 181 ASLTDNANTGVVKLGDGRVVCLTETQKGSIMIDPTTLETMGKFEYSDSLGGLIHSAHPIV 240
           ASLTDNANTGVVKLGDGRVVCLTETQKGSIMIDP TLET+GKFEYSDSLGGLIHSAHPIV
Sbjct: 181 ASLTDNANTGVVKLGDGRVVCLTETQKGSIMIDPDTLETVGKFEYSDSLGGLIHSAHPIV 240

Query: 241 TDSEFLTLLPDLLNPGYLVVRMEPGSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPE 300
           TDSEFLTLLPDLLNPGYLVVRMEP SNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPE
Sbjct: 241 TDSEFLTLLPDLLNPGYLVVRMEPSSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPE 300

Query: 301 MPLRYCAQNLLRAEPTPLYKFEWRPESKAFVHVMCKASGTIVASVEVPLFITFHFINAYE 360
           MPLRYCAQNLL+AEPTPLYKFEWRPESKAF+HVMCKASG IVASVEVPLFITFHFINAYE
Sbjct: 301 MPLRYCAQNLLKAEPTPLYKFEWRPESKAFMHVMCKASGNIVASVEVPLFITFHFINAYE 360

Query: 361 ERDEKGRITAVIADCCEHNANPVILDRLRLHNLRRSLKHPLPDAR 402
           ERDEKGRITAVIADCCEHNANPVILDRLRLHNLR SLK+PLPDAR
Sbjct: 361 ERDEKGRITAVIADCCEHNANPVILDRLRLHNLRDSLKYPLPDAR 405

[Close]

BLAST of Lsi08G010850 vs. NCBI nr
Match: NP_001284455.1 (carotenoid cleavage dioxygenase 8 homolog B, chloroplastic [Cucumis melo] >AHY18725.1 carotenoid cleavage dioxygenase 8 [Cucumis melo])

HSP 1 Score: 746.9 bits (1927), Expect = 9.4e-212
Identity = 365/406 (89.90%), Postives = 378/406 (93.10%), Query Frame = 0


Query: 1   MMASMATGSCIISSFNFKTNHLILPIH--PSKPT--NFTIRSIASPIHRRSSVAVPLPEI 60
           MMASMA GSCIIS FNFKT H +LPI    SKPT  NFTIRSIASP+   S V VPLPEI
Sbjct: 1   MMASMAIGSCIISPFNFKTKHPLLPIQQPSSKPTLSNFTIRSIASPVRPPSPVVVPLPEI 60

Query: 61  DTSGLPNHVAWTSVRQHRWEGELSVQGQLPRWL-SGTYLRNGPGLWHIGEYNFRHLFDGY 120
           DT   PNHVAWTS+RQ RWEGELSVQG LP WL SGTYLRNGPGLW+IG+YNFRHLFDGY
Sbjct: 61  DTFDAPNHVAWTSIRQDRWEGELSVQGHLPSWLVSGTYLRNGPGLWNIGDYNFRHLFDGY 120

Query: 121 ATMVKLHFDDGRLTAGHRQIESDAYKAAMKNQKICYREFSEVPKSDNFLAYVGELANLFS 180
           A +VKLHFD+GRL AGHRQIES+AYKAAMKNQKICYREFSEVPK+DNFLAYVGELANLFS
Sbjct: 121 AMIVKLHFDNGRLIAGHRQIESNAYKAAMKNQKICYREFSEVPKADNFLAYVGELANLFS 180

Query: 181 GASLTDNANTGVVKLGDGRVVCLTETQKGSIMIDPTTLETMGKFEYSDSLGGLIHSAHPI 240
           GASLTDNANTGVVKLGDGRVVCLTETQKGSIMIDP TLET+GKFEYSDSLGGLIHSAHPI
Sbjct: 181 GASLTDNANTGVVKLGDGRVVCLTETQKGSIMIDPDTLETVGKFEYSDSLGGLIHSAHPI 240

Query: 241 VTDSEFLTLLPDLLNPGYLVVRMEPGSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVP 300
           VTDSEFLTLLPDLLNPGYLVVRMEP SNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVP
Sbjct: 241 VTDSEFLTLLPDLLNPGYLVVRMEPSSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVP 300

Query: 301 EMPLRYCAQNLLRAEPTPLYKFEWRPESKAFVHVMCKASGTIVASVEVPLFITFHFINAY 360
           EMPLRYCAQNLL+AEPTPLYKFEWRPESKAF+HVMCKASG IVASVEVPLFITFHFINAY
Sbjct: 301 EMPLRYCAQNLLKAEPTPLYKFEWRPESKAFMHVMCKASGNIVASVEVPLFITFHFINAY 360

Query: 361 EERDEKGRITAVIADCCEHNANPVILDRLRLHNLRRSLKHPLPDAR 402
           EERDEKGRITAVIADCCEHNANPVILDRLRLHNLR SLK+PLPDAR
Sbjct: 361 EERDEKGRITAVIADCCEHNANPVILDRLRLHNLRDSLKYPLPDAR 406

[Close]

BLAST of Lsi08G010850 vs. NCBI nr
Match: KAG6598778.1 (Carotenoid cleavage dioxygenase 8-like B, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 726.9 bits (1875), Expect = 1.0e-205
Identity = 353/403 (87.59%), Postives = 373/403 (92.56%), Query Frame = 0


Query: 2   MASMATGSCIISSFNFKTNHLILPIHPSKPT--NFTIRSIASPIHRRSSVAVPLPEIDTS 61
           MASMA GSCI    +FK  +  LP HP K T  NFTIRSIASPI     V VPLP+IDTS
Sbjct: 1   MASMAAGSCI----SFKPYNPCLPFHPPKRTVSNFTIRSIASPIRPSPPVVVPLPDIDTS 60

Query: 62  GLPNHVAWTSVRQHRWEGELSVQGQLPRWLSGTYLRNGPGLWHIGEYNFRHLFDGYATMV 121
             P HVAWTS+RQ RWEG+LSVQGQLPRWL+GTYLRNGPG+W+IG YNFRHLFDGYAT+V
Sbjct: 61  IPPTHVAWTSIRQDRWEGQLSVQGQLPRWLNGTYLRNGPGVWNIGNYNFRHLFDGYATIV 120

Query: 122 KLHFDDGRLTAGHRQIESDAYKAAMKNQKICYREFSEVPKSDNFLAYVGELANLFSGASL 181
           KLHF+DGRL AGHRQIES+AYKAAMKNQK+CYREFSEVPK+DNFLAYVGELANLFSG SL
Sbjct: 121 KLHFEDGRLIAGHRQIESNAYKAAMKNQKVCYREFSEVPKTDNFLAYVGELANLFSGVSL 180

Query: 182 TDNANTGVVKLGDGRVVCLTETQKGSIMIDPTTLETMGKFEYSDSLGGLIHSAHPIVTDS 241
           TDNANTGVVKLGD RVVCLTETQKGSI+I+P+TLET+GKFEYSDSLGGLIHSAHPIVTDS
Sbjct: 181 TDNANTGVVKLGDSRVVCLTETQKGSIIINPSTLETIGKFEYSDSLGGLIHSAHPIVTDS 240

Query: 242 EFLTLLPDLLNPGYLVVRMEPGSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPEMPL 301
           EFLTLLPDLLNPGYLVVRMEPGSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPEMPL
Sbjct: 241 EFLTLLPDLLNPGYLVVRMEPGSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPEMPL 300

Query: 302 RYCAQNLLRAEPTPLYKFEWRPESKAFVHVMCKASGTIVASVEVPLFITFHFINAYEERD 361
           RYCAQNLLRAEPTPLYKFEWRPESKAF+HVMCKASG+IVASVEVPLFITFHFINAYEERD
Sbjct: 301 RYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGSIVASVEVPLFITFHFINAYEERD 360

Query: 362 EKGRITAVIADCCEHNANPVILDRLRLHNLRR-SLKHPLPDAR 402
           EKGRITAVIADCCEHNANP+ILDRLRLHNLRR SLKH LPDAR
Sbjct: 361 EKGRITAVIADCCEHNANPLILDRLRLHNLRRFSLKHALPDAR 399

[Close]

BLAST of Lsi08G010850 vs. TAIR 10
Match: AT4G32810.1 (carotenoid cleavage dioxygenase 8 )

HSP 1 Score: 494.2 bits (1271), Expect = 1.0e-139
Identity = 240/365 (65.75%), Postives = 288/365 (78.90%), Query Frame = 0


Query: 47  SSVAVPLPEID--TSGLPNHVAWTSVRQHRWEGELSVQGQLPRWLSGTYLRNGPGLWHIG 106
           S+   PLPE +        HVAWTSV+Q  WEGEL+VQG++P WL+GTYLRNGPGLW+IG
Sbjct: 61  SAAPSPLPEKEKVEGERRCHVAWTSVQQENWEGELTVQGKIPTWLNGTYLRNGPGLWNIG 120

Query: 107 EYNFRHLFDGYATMVKLHFDDGRLTAGHRQIESDAYKAAMKNQKICYREFSEVPKS---- 166
           +++FRHLFDGY+T+VKL FD GR+ A HR +ESDAYKAA K+ ++CYREFSE PKS    
Sbjct: 121 DHDFRHLFDGYSTLVKLQFDGGRIFAAHRLLESDAYKAAKKHNRLCYREFSETPKSVIIN 180

Query: 167 DNFLAYVGELANLFSGASLTDNANTGVVKLGDGRVVCLTETQKGSIMIDPTTLETMGKFE 226
            N  + +GE+  LFSG SLTDNANTGV+KLGDGRV+CLTETQKGSI++D  TLET+GKFE
Sbjct: 181 KNPFSGIGEIVRLFSGESLTDNANTGVIKLGDGRVMCLTETQKGSILVDHETLETIGKFE 240

Query: 227 YSDSLGG-LIHSAHPIVTDSEFLTLLPDLLNPGYLVVRMEPGSNERKVIGRVNCKGGP-A 286
           Y D L   +I SAHPIVT++E  TL+PDL+ PGY VVRME GSN+R+V+GRV C+ G   
Sbjct: 241 YDDVLSDHMIQSAHPIVTETEMWTLIPDLVKPGYRVVRMEAGSNKREVVGRVRCRSGSWG 300

Query: 287 PGWVHSFPVTENYVVVPEMPLRYCAQNLLRAEPTPLYKFEWRPESKAFVHVMCKASGTIV 346
           PGWVHSF VTENYVV+PEMPLRY  +NLLRAEPTPLYKFEW P+  AF+HVM K +G +V
Sbjct: 301 PGWVHSFAVTENYVVIPEMPLRYSVKNLLRAEPTPLYKFEWCPQDGAFIHVMSKLTGEVV 360

Query: 347 ASVEVPLFITFHFINAYEE-RDEKGRITAVIADCCEHNANPVILDRLRLHNLRRSLKHP- 402
           ASVEVP ++TFHFINAYEE ++  G+ T +IADCCEHNA+  ILD LRL  LR S  H  
Sbjct: 361 ASVEVPAYVTFHFINAYEEDKNGDGKATVIIADCCEHNADTRILDMLRLDTLRSSHGHDV 420

[Close]

BLAST of Lsi08G010850 vs. TAIR 10
Match: AT3G24220.1 (nine-cis-epoxycarotenoid dioxygenase 6 )

HSP 1 Score: 84.7 bits (208), Expect = 1.9e-16
Identity = 97/352 (27.56%), Postives = 152/352 (43.18%), Query Frame = 0


Query: 38  SIASPIHRRSSVAVPL-PEIDTSGLPNHVAWTSVRQHRWEGELSVQGQLPRWLSGTYLRN 97
           SI  P+ +   +  P  P +  SG      +  V +   +  L V GQ+P  L G Y+RN
Sbjct: 80  SIVIPMEQNRPLPKPTDPAVQLSG-----NFAPVNECPVQNGLEVVGQIPSCLKGVYIRN 139

Query: 98  GPGLWHIGEYNFRHLFDGYATM--VKLHFDDG-----RLTAGHRQIESDAYKAAMKNQKI 157
           G            HLFDG   +  V + FD+      R T  +R ++  A   ++  + I
Sbjct: 140 GANPM-FPPLAGHHLFDGDGMIHAVSIGFDNQVSYSCRYTKTNRLVQETALGRSVFPKPI 199

Query: 158 C-YREFSEVPKSDNFLAYVGELANLFSGASLTDNANTGVVKLGDGRVVCLTETQ-KGSIM 217
                 S + +   F A  G    L  G      AN GVV   +GR++ ++E      + 
Sbjct: 200 GELHGHSGLARLALFTARAG--IGLVDGTRGMGVANAGVV-FFNGRLLAMSEDDLPYQVK 259

Query: 218 ID-PTTLETMGKFEYSDSLGGLIHSAHPIV--TDSEFLTLLPDLLNPGYL--VVRMEPGS 277
           ID    LET+G+F + D +   +  AHP V  T  +  TL  ++L   +L  +     G 
Sbjct: 260 IDGQGDLETIGRFGFDDQIDSSV-IAHPKVDATTGDLHTLSYNVLKKPHLRYLKFNTCGK 319

Query: 278 NERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPEMPLRYCAQNLLRAEPTPLYKFEWRPE 337
             R V   +     P P  +H F +TEN+VV+P+  + +    ++R     +Y      E
Sbjct: 320 KTRDVEITL-----PEPTMIHDFAITENFVVIPDQQMVFKLSEMIRGGSPVIYV----KE 379

Query: 338 SKAFVHVMCK--ASGTIVASVEVPLFITFHFINAYEERDEKGRITAVIADCC 373
             A   V+ K   +G+ +  V+VP    FH  NA+EER E+G    V+   C
Sbjct: 380 KMARFGVLSKQDLTGSDINWVDVPDCFCFHLWNAWEERTEEGDPVIVVIGSC 412

[Close]

BLAST of Lsi08G010850 vs. TAIR 10
Match: AT3G14440.1 (nine-cis-epoxycarotenoid dioxygenase 3 )

HSP 1 Score: 69.3 bits (168), Expect = 8.1e-12
Identity = 74/317 (23.34%), Postives = 133/317 (41.96%), Query Frame = 0


Query: 52  PLPEIDTSGLPNHVAWTSVRQHRWEGELSVQGQLPRWLSGTYLRNGPGLWHIGEYNFRHL 111
           PLP+     +     +  V +      L V G+LP  + G Y+RNG    H       H 
Sbjct: 112 PLPKTADPSVQIAGNFAPVNEQPVRRNLPVVGKLPDSIKGVYVRNGANPLH-EPVTGHHF 171

Query: 112 FDGYATMVKLHFDDGRLTAGHRQIESDAY-KAAMKNQKICYREFSEVPKSDN----FLAY 171
           FDG   +  + F+ G  +   R  +++ + +     + +  +   E+          L Y
Sbjct: 172 FDGDGMVHAVKFEHGSASYACRFTQTNRFVQERQLGRPVFPKAIGELHGHTGIARLMLFY 231

Query: 172 VGELANLFSGASLTDNANTGVVKLGDGRVVCLTETQ-KGSIMIDPT-TLETMGKFEYSDS 231
               A +   A  T  AN G+V   +GR++ ++E      + I P   L+T+G+F++   
Sbjct: 232 ARAAAGIVDPAHGTGVANAGLVYF-NGRLLAMSEDDLPYQVQITPNGDLKTVGRFDFDGQ 291

Query: 232 LGGLIHSAHPIV--TDSEFLTLLPDLLNPGYL-VVRMEP-GSNERKVIGRVNCKGGPAPG 291
           L   +  AHP V     E   L  D+++  YL   R  P G+    V  +++      P 
Sbjct: 292 LESTM-IAHPKVDPESGELFALSYDVVSKPYLKYFRFSPDGTKSPDVEIQLD-----QPT 351

Query: 292 WVHSFPVTENYVVVPEMPLRYCAQNLLRAEPTPLYKFEWRPESKAFVHVMCKASGTIVAS 351
            +H F +TEN+VVVP+  + +    ++R     +Y  +    ++  +        + +  
Sbjct: 352 MMHDFAITENFVVVPDQQVVFKLPEMIRGGSPVVY--DKNKVARFGILDKYAEDSSNIKW 411

Query: 352 VEVPLFITFHFINAYEE 358
           ++ P    FH  NA+EE
Sbjct: 412 IDAPDCFCFHLWNAWEE 418

[Close]

BLAST of Lsi08G010850 vs. TAIR 10
Match: AT1G78390.1 (nine-cis-epoxycarotenoid dioxygenase 9 )

HSP 1 Score: 65.9 bits (159), Expect = 9.0e-11
Identity = 70/294 (23.81%), Postives = 126/294 (42.86%), Query Frame = 0


Query: 79  LSVQGQLPRWLSGTYLRNGPGLWHIGEYNFRHLFDGYATMVKLHFDDGRLTAGHRQIESD 138
           L V G +P  + G Y+RNG    H    +  HLFDG   +  + FD+G ++   R  E++
Sbjct: 199 LPVTGTVPECIQGVYVRNGANPLH-KPVSGHHLFDGDGMVHAVRFDNGSVSYACRFTETN 258

Query: 139 AYKAAMKNQKICYREFSEVPKSDNFL-AYVG-------ELANLFSGASLTDN---ANTGV 198
                +  ++ C R     PK+   L  ++G           LF     T     AN G+
Sbjct: 259 ----RLVQERECGRPV--FPKAIGELHGHLGIAKLMLFNTRGLFGLVDPTGGLGVANAGL 318

Query: 199 VKLGDGRVVCLTETQ-KGSIMIDPT-TLETMGKFEYSDSLGGLIHSAHPIVTDS--EFLT 258
           V   +G ++ ++E      + +  T  LET G++++   L   +  AHP +     E   
Sbjct: 319 VYF-NGHLLAMSEDDLPYHVKVTQTGDLETSGRYDFDGQLKSTM-IAHPKIDPETRELFA 378

Query: 259 LLPDLLNPGYLVVRMEPGSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPEMPLRYCA 318
           L  D+++  YL         E+     +       P  +H F +TEN+VV+P+  + +  
Sbjct: 379 LSYDVVSKPYLKYFRFTSDGEKSPDVEIPL---DQPTMIHDFAITENFVVIPDQQVVFRL 438

Query: 319 QNLLRAEPTPLYKFEWRPESKAFVHVMCKASGTIVASVEVPLFITFHFINAYEE 358
             ++R     +Y  + + +S+  +        + +  +EVP    FH  N++EE
Sbjct: 439 PEMIRGGSPVVY--DEKKKSRFGILNKNAKDASSIQWIEVPDCFCFHLWNSWEE 478

[Close]

BLAST of Lsi08G010850 vs. TAIR 10
Match: AT4G18350.1 (nine-cis-epoxycarotenoid dioxygenase 2 )

HSP 1 Score: 59.3 bits (142), Expect = 8.4e-09
Identity = 72/345 (20.87%), Postives = 140/345 (40.58%), Query Frame = 0


Query: 54  PEIDTSGLPNHVAWTSVRQHRWEGELSVQGQLPRWLSGTYLRNG------PGLWHIGEYN 113
           P +  +G  + V  +SVR++     L+V+G +P  + G Y+RNG      P   H     
Sbjct: 102 PRVQIAGNYSPVPESSVRRN-----LTVEGTIPDCIDGVYIRNGANPMFEPTAGH----- 161

Query: 114 FRHLFDGYATMVKLHFDDGRLTAGHRQIESDAYKAAMK-NQKICYREFSEVPKSDN---- 173
             HLFDG   +  +   +G  +   R  +++      +  + +  +   E+         
Sbjct: 162 --HLFDGDGMVHAVKITNGSASYACRFTKTERLVQEKRLGRPVFPKAIGELHGHSGIARL 221

Query: 174 FLAYVGELANLFSGASLTDNANTGVVKLGDGRVVCLTETQKGSIMIDPT-TLETMGKFEY 233
            L Y   L  L +  +    AN G+V   +  +    +     + I  T  L+T+G++++
Sbjct: 222 MLFYARGLCGLINNQNGVGVANAGLVYFNNRLLAMSEDDLPYQLKITQTGDLQTVGRYDF 281

Query: 234 SDSLGGLIHSAHPIV--TDSEFLTLLPDLLNPGYL-VVRMEPGSNERKVIGRVNCKGGPA 293
              L   +  AHP +     E   L  D++   YL   R  P   +   +  +  +    
Sbjct: 282 DGQLKSAM-IAHPKLDPVTKELHALSYDVVKKPYLKYFRFSPDGVKSPEL-EIPLE---T 341

Query: 294 PGWVHSFPVTENYVVVPEMPLRYCAQNLLRAEPTPLYKFEWRPESKAFVHVMCKASGTIV 353
           P  +H F +TEN+VV+P+  + +    ++  + +P+  F+    S+  +        + +
Sbjct: 342 PTMIHDFAITENFVVIPDQQVVFKLGEMISGK-SPVV-FDGEKVSRLGIMPKDATEASQI 401

Query: 354 ASVEVPLFITFHFINAYEERDEKGRITAVIADCCEHNANPVILDR 384
             V  P    FH  NA+E  + +     V+   C   A+ +  +R
Sbjct: 402 IWVNSPETFCFHLWNAWESPETE---EIVVIGSCMSPADSIFNER 424

The following BLAST results are available for this feature:

BLAST of Lsi08G010850 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LIY8	1.4e-157	75.44	Carotenoid cleavage dioxygenase 8 homolog B, chloroplastic OS=Oryza sativa subsp...	[more]
Q8VY26	1.4e-138	65.75	Carotenoid cleavage dioxygenase 8, chloroplastic OS=Arabidopsis thaliana OX=3702...	[more]
Q93VD5	2.4e-117	59.53	Carotenoid cleavage dioxygenase 8 homolog A, chloroplastic OS=Oryza sativa subsp...	[more]
Q9VFS2	8.4e-30	26.49	Carotenoid isomerooxygenase OS=Drosophila melanogaster OX=7227 GN=ninaB PE=1 SV=...	[more]
A9C3R8	1.1e-29	28.45	Retinal Mueller cells isomerohydrolase OS=Danio rerio OX=7955 GN=rpe65c PE=1 SV=...	[more]

BLAST of Lsi08G010850 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LS17	1.1e-213	90.59	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G373590 PE=3 SV=1	[more]
A0A5A7VHT5	7.0e-213	89.88	Carotenoid cleavage dioxygenase 8-like protein B OS=Cucumis melo var. makuwa OX=...	[more]
A0A1S3BBP5	7.0e-213	89.88	carotenoid cleavage dioxygenase 8 homolog B, chloroplastic isoform X1 OS=Cucumis...	[more]
A0A023VSV7	4.5e-212	89.90	Carotenoid cleavage dioxygenase 8 OS=Cucumis melo OX=3656 GN=CCD8 PE=2 SV=1	[more]
A0A6J1HGQ6	5.4e-205	87.10	carotenoid cleavage dioxygenase 8 homolog B, chloroplastic-like OS=Cucurbita mos...	[more]

BLAST of Lsi08G010850 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038885421.1	1.0e-218	92.56	carotenoid cleavage dioxygenase 8 homolog B, chloroplastic [Benincasa hispida]	[more]
XP_004148387.1	2.2e-213	90.59	carotenoid cleavage dioxygenase 8 homolog B, chloroplastic [Cucumis sativus] >KG...	[more]
XP_008445014.1	1.4e-212	89.88	PREDICTED: carotenoid cleavage dioxygenase 8 homolog B, chloroplastic isoform X1...	[more]
NP_001284455.1	9.4e-212	89.90	carotenoid cleavage dioxygenase 8 homolog B, chloroplastic [Cucumis melo] >AHY18...	[more]
KAG6598778.1	1.0e-205	87.59	Carotenoid cleavage dioxygenase 8-like B, chloroplastic, partial [Cucurbita argy...	[more]

BLAST of Lsi08G010850 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G32810.1	1.0e-139	65.75	carotenoid cleavage dioxygenase 8	[more]
AT3G24220.1	1.9e-16	27.56	nine-cis-epoxycarotenoid dioxygenase 6	[more]
AT3G14440.1	8.1e-12	23.34	nine-cis-epoxycarotenoid dioxygenase 3	[more]
AT1G78390.1	9.0e-11	23.81	nine-cis-epoxycarotenoid dioxygenase 9	[more]
AT4G18350.1	8.4e-09	20.87	nine-cis-epoxycarotenoid dioxygenase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004294	Carotenoid oxygenase	PFAM	PF03055	RPE65	coord: 78..393 e-value: 8.0E-58 score: 196.7
IPR004294	Carotenoid oxygenase	PANTHER	PTHR10543	BETA-CAROTENE DIOXYGENASE	coord: 64..402
None	No IPR available	PANTHER	PTHR10543:SF118	CAROTENOID CLEAVAGE DIOXYGENASE-RELATED	coord: 64..402

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi08G010850.1	Lsi08G010850.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016121	carotene catabolic process
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0010436	carotenoid dioxygenase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016702	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen