Lsi08G009890 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi08G009890
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	RING-type E3 ubiquitin transferase
Location	chr08: 18348886 .. 18349803 (+)
RNA-Seq Expression	Lsi08G009890
Synteny	Lsi08G009890

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGTTACAATTAGCAGAAAACTTTTCCCTGTTGCTACTGGAACTACCCAACCAAACACCAATTGTTCCAGCTGCCCTTACGACTGCCCTTTTGACTGCTACGGTATTCCAGATTTCTACTTCTCACCGCCGCCGCCGCCGCCCCCGCCTACACTCACCGATCACCAAAAACAGCTTCCAACTATAATCATCATCCTAATTCTGTTCGTAGGATTTTTCATCGCTTTTAGTTTCTATATCATCGTCGTAAAATGCCGATCTTGGCAAAGTGGCTCCGGCAACGAAGGGACAGAGGCGTTGCAATCGGACGGCGGAGAAGGAGAGTTTATGAACGAGAATCAAATCGACCATCCAATTTGGTTCATCACCACCGTTGGACTGCAGCAATCAGTCATCAATTCGATTACGGTTTGCAAGTACAAGAAGAGCGAAGGTTTAATTGAAGGAACAGATTGCTCTGTTTGTTTGAGTGAATTTCAAGAAGACGAAATGCTTCGGCTATTACCCAAATGCAGCCACGCGTTTCATATTGGGTGTGTTGATACTTGGCTCAGAACTCATACCACTTGCCCTCTCTGTCGAGCTCATATTCTTACCGATTTCACGAACTCAAACTCTGCGAGGCCACCCAATTTAGGCCCTGTGAATCAAAACGAAGCTAATTTGAGTGTAAATGAGGAAACCCAGATGGAGAATGATGAGATTAATGGCGAAGTTTTAGTGGAAAATGAGGGGGTTGTTTCTAGTGGGAGCTCTGAAAACAGAGGAGATGTTGTTGATGAACAGAGAGAAGTTGGGGATCTTCAGATTGGGGAGATAGTTCCAAAAGAAGGGAATTTTGAATCTGATGAATCTAAAGTTGAAGGAAACGAAAGTATGAGGAACAATGAGATGGATTCTTCTTCAGCAACATGA

mRNA sequence

ATGGCGGTTACAATTAGCAGAAAACTTTTCCCTGTTGCTACTGGAACTACCCAACCAAACACCAATTGTTCCAGCTGCCCTTACGACTGCCCTTTTGACTGCTACGGTATTCCAGATTTCTACTTCTCACCGCCGCCGCCGCCGCCCCCGCCTACACTCACCGATCACCAAAAACAGCTTCCAACTATAATCATCATCCTAATTCTGTTCGTAGGATTTTTCATCGCTTTTAGTTTCTATATCATCGTCGTAAAATGCCGATCTTGGCAAAGTGGCTCCGGCAACGAAGGGACAGAGGCGTTGCAATCGGACGGCGGAGAAGGAGAGTTTATGAACGAGAATCAAATCGACCATCCAATTTGGTTCATCACCACCGTTGGACTGCAGCAATCAGTCATCAATTCGATTACGGTTTGCAAGTACAAGAAGAGCGAAGGTTTAATTGAAGGAACAGATTGCTCTGTTTGTTTGAGTGAATTTCAAGAAGACGAAATGCTTCGGCTATTACCCAAATGCAGCCACGCGTTTCATATTGGGTGTGTTGATACTTGGCTCAGAACTCATACCACTTGCCCTCTCTGTCGAGCTCATATTCTTACCGATTTCACGAACTCAAACTCTGCGAGGCCACCCAATTTAGGCCCTGTGAATCAAAACGAAGCTAATTTGAGTGTAAATGAGGAAACCCAGATGGAGAATGATGAGATTAATGGCGAAGTTTTAGTGGAAAATGAGGGGGTTGTTTCTAGTGGGAGCTCTGAAAACAGAGGAGATGTTGTTGATGAACAGAGAGAAGTTGGGGATCTTCAGATTGGGGAGATAGTTCCAAAAGAAGGGAATTTTGAATCTGATGAATCTAAAGTTGAAGGAAACGAAAGTATGAGGAACAATGAGATGGATTCTTCTTCAGCAACATGA

Coding sequence (CDS)

ATGGCGGTTACAATTAGCAGAAAACTTTTCCCTGTTGCTACTGGAACTACCCAACCAAACACCAATTGTTCCAGCTGCCCTTACGACTGCCCTTTTGACTGCTACGGTATTCCAGATTTCTACTTCTCACCGCCGCCGCCGCCGCCCCCGCCTACACTCACCGATCACCAAAAACAGCTTCCAACTATAATCATCATCCTAATTCTGTTCGTAGGATTTTTCATCGCTTTTAGTTTCTATATCATCGTCGTAAAATGCCGATCTTGGCAAAGTGGCTCCGGCAACGAAGGGACAGAGGCGTTGCAATCGGACGGCGGAGAAGGAGAGTTTATGAACGAGAATCAAATCGACCATCCAATTTGGTTCATCACCACCGTTGGACTGCAGCAATCAGTCATCAATTCGATTACGGTTTGCAAGTACAAGAAGAGCGAAGGTTTAATTGAAGGAACAGATTGCTCTGTTTGTTTGAGTGAATTTCAAGAAGACGAAATGCTTCGGCTATTACCCAAATGCAGCCACGCGTTTCATATTGGGTGTGTTGATACTTGGCTCAGAACTCATACCACTTGCCCTCTCTGTCGAGCTCATATTCTTACCGATTTCACGAACTCAAACTCTGCGAGGCCACCCAATTTAGGCCCTGTGAATCAAAACGAAGCTAATTTGAGTGTAAATGAGGAAACCCAGATGGAGAATGATGAGATTAATGGCGAAGTTTTAGTGGAAAATGAGGGGGTTGTTTCTAGTGGGAGCTCTGAAAACAGAGGAGATGTTGTTGATGAACAGAGAGAAGTTGGGGATCTTCAGATTGGGGAGATAGTTCCAAAAGAAGGGAATTTTGAATCTGATGAATCTAAAGTTGAAGGAAACGAAAGTATGAGGAACAATGAGATGGATTCTTCTTCAGCAACATGA

Protein sequence

MAVTISRKLFPVATGTTQPNTNCSSCPYDCPFDCYGIPDFYFSPPPPPPPPTLTDHQKQLPTIIIILILFVGFFIAFSFYIIVVKCRSWQSGSGNEGTEALQSDGGEGEFMNENQIDHPIWFITTVGLQQSVINSITVCKYKKSEGLIEGTDCSVCLSEFQEDEMLRLLPKCSHAFHIGCVDTWLRTHTTCPLCRAHILTDFTNSNSARPPNLGPVNQNEANLSVNEETQMENDEINGEVLVENEGVVSSGSSENRGDVVDEQREVGDLQIGEIVPKEGNFESDESKVEGNESMRNNEMDSSSAT

Homology

[Close]

BLAST of Lsi08G009890 vs. ExPASy Swiss-Prot
Match: Q8LFY8 (RING-H2 finger protein ATL54 OS=Arabidopsis thaliana OX=3702 GN=ATL54 PE=2 SV=2)

HSP 1 Score: 174.5 bits (441), Expect = 1.9e-42
Identity = 124/319 (38.87%), Postives = 163/319 (51.10%), Query Frame = 0


Query: 1   MAVTISRKLFPVATGTTQPNTNCSS--CPYDCPFDCYGIPDFY-FSP----------PPP 60
           MA    RKLFP     T    +CS+  C   CP++CY  PD+Y  SP          P P
Sbjct: 1   MARKKHRKLFPTLASETNKTLDCSNGVCDPICPYNCYPEPDYYTISPQLPPWSSSPQPSP 60

Query: 61  PPPPTLT-------DHQKQLPTIIIILILFVGFFIAFSFYIIVVKCRSWQSGSGNEGTEA 120
            P P+++       D    L  I II I      I  + + +V K  S      N+GT  
Sbjct: 61  CPSPSISAVYLPSQDSSSSLDAISIITITGAVLAILLTGFFLVAKFFSDSVNRVNQGTYQ 120

Query: 121 LQSDGG----EGEFMNENQIDHPIWFITTVGLQQSVINSITVCKYKKSEGLIEGTDCSVC 180
             ++      E EF +  Q+DHPIW I T GLQQS+INSIT+C YK+ +GLIE TDC VC
Sbjct: 121 SDNEDNDTVMEEEFQDREQVDHPIWLIRTTGLQQSIINSITICNYKRGDGLIERTDCPVC 180

Query: 181 LSEFQEDEMLRLLPKCSHAFHIGCVDTWLRTHTTCPLCRAHILTDFTNSNSARPPNLGPV 240
           L+EF+EDE LRLLPKC+HAFHI C+DTWL +HT CPLCRA I       +   P   GPV
Sbjct: 181 LNEFEEDESLRLLPKCNHAFHISCIDTWLSSHTNCPLCRAGI----AMISVTTPRYSGPV 240

Query: 241 NQNEANLSVNEETQMENDEINGEVLVENEGVVSSGSSENRGDVVDEQREVGDLQIGEIVP 296
                       + +END ++ E           G  ENR D   ++ +  D++I EI  
Sbjct: 241 EVTPG----GSGSHLENDGVDEE---------DHGEIENRVDSDFKESDDSDIRI-EIYR 299

[Close]

BLAST of Lsi08G009890 vs. ExPASy Swiss-Prot
Match: Q9LX93 (E3 ubiquitin-protein ligase RING1 OS=Arabidopsis thaliana OX=3702 GN=ATL55 PE=1 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 4.4e-36
Identity = 81/159 (50.94%), Postives = 104/159 (65.41%), Query Frame = 0


Query: 44  PPPPPPPPTLTDHQKQLPTIIII---LILFVGFFIAFSFYIIVVKCRSWQSGSGNEGTEA 103
           PPPPPPPP   +    LPT I +   +ILF+  F+ F  Y+ + + R   + S   G   
Sbjct: 26  PPPPPPPPPPRELSLLLPTSICVVGSIILFL--FLVFFLYLHITQQRRISAASVTPGDTN 85

Query: 104 LQSDGGEGEFMNENQIDHPIWFITTVGLQQSVINSITVCKYKKSEGLIEGTDCSVCLSEF 163
            Q D  E E  + +   H +W I TVGL +S INSITV  +KK EG+I+GT+CSVCL+EF
Sbjct: 86  QQEDEDETEERDFSDFHH-VWQIPTVGLHRSAINSITVVGFKKGEGIIDGTECSVCLNEF 145

Query: 164 QEDEMLRLLPKCSHAFHIGCVDTWLRTHTTCPLCRAHIL 200
           +EDE LRLLPKCSHAFH+ C+DTWL +H  CPLCRA +L
Sbjct: 146 EEDESLRLLPKCSHAFHLNCIDTWLLSHKNCPLCRAPVL 181

[Close]

BLAST of Lsi08G009890 vs. ExPASy Swiss-Prot
Match: Q9LF64 (RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 8.4e-27
Identity = 93/267 (34.83%), Postives = 139/267 (52.06%), Query Frame = 0


Query: 27  PYDCPFDC-YGIPDFYFSP------PPPPPPPTLTDHQKQ-----LPTIIIILILFVGFF 86
           PYD   DC  GI + Y         PPPPP   L D          P +I ++ +     
Sbjct: 10  PYDSYNDCSQGICNIYCPQWCYLIFPPPPPSFFLDDDSSSSSSSFSPLLIALIGILTSAL 69

Query: 87  IAFSFYIIVVK-CRSWQSGSGNEGTEALQSDGGEGEFMNENQIDHPIWFITTVGLQQSVI 146
           I  S+Y ++ K C      S +E      +  GEG F +  +I          GL +S+I
Sbjct: 70  ILVSYYTLISKYCHRHHQTSSSETLNL--NHNGEGFFSSTQRIS-----TNGDGLNESMI 129

Query: 147 NSITVCKYKKSEGLIEGTDCSVCLSEFQEDEMLRLLPKCSHAFHIGCVDTWLRTHTTCPL 206
            SITV KYK  +G ++G+DCSVCLSEF+E+E LRLLPKC+HAFH+ C+DTWL++H+ CPL
Sbjct: 130 KSITVYKYKSGDGFVDGSDCSVCLSEFEENESLRLLPKCNHAFHLPCIDTWLKSHSNCPL 189

Query: 207 CRAHILTDFTNSNSARPPNLGPVNQNEANL-----SVNEET------QMENDEINGEVLV 266
           CRA + T   N  ++   N+  V  N++N      SV+E        + +  + +  V++
Sbjct: 190 CRAFV-TGVNNPTASVGQNVSVVVANQSNSAHQTGSVSEINLNLAGYESQTGDFDSVVVI 249

Query: 267 ENEGVVSSGS-SENRGDVVDEQREVGD 269
           E+  + S  S + +   + +E+RE  D
Sbjct: 250 EDLEIGSRNSDARSELQLPEERRETKD 268

[Close]

BLAST of Lsi08G009890 vs. ExPASy Swiss-Prot
Match: Q9SRQ8 (RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2)

HSP 1 Score: 121.3 bits (303), Expect = 1.9e-26
Identity = 101/295 (34.24%), Postives = 137/295 (46.44%), Query Frame = 0


Query: 11  PVATGTTQPN-TNCSS--CPYDCPFDCYGIPDFYFSPPPPPPPPTLTDHQKQ-----LPT 70
           P   GTT  +  +CS   C   CP  CY I        PPPP   L D          P 
Sbjct: 7   PNPWGTTYDSYRDCSQGVCSVYCPQWCYVI-------FPPPPSFYLDDEDDSSSSDFSPL 66

Query: 71  IIIILILFVGFFIAFSFYIIVVK-CRSWQSGSGNEGTEALQSDGGEGEFM-----NENQI 130
           +I ++ +    FI  S+Y ++ K C   +  S +    A+     +  +      N N  
Sbjct: 67  LIALIGILASAFILVSYYTLISKYCHRRRHNSSSTSAAAINRISSDYTWQGTNNNNNNGA 126

Query: 131 DHPIWFI---TTVGLQQSVINSITVCKYKKSEGLIEGTDCSVCLSEFQEDEMLRLLPKCS 190
            +P   I      GL +S+I SITV KY+K +G +E +DCSVCLSEFQE+E LRLLPKC+
Sbjct: 127 TNPNQTIGGGGGDGLDESLIKSITVYKYRKMDGFVESSDCSVCLSEFQENESLRLLPKCN 186

Query: 191 HAFHIGCVDTWLRTHTTCPLCRAHILT-------DFTN-------------SNSARPPNL 250
           HAFH+ C+DTWL++H+ CPLCRA I+T       D TN              +S    NL
Sbjct: 187 HAFHVPCIDTWLKSHSNCPLCRAFIVTSSAVEIVDLTNQQIVTENNSISTGDDSVVVVNL 246

Query: 251 GPVNQNEANLSVNEETQMENDEINGEVLVENEGVVSSGSSENRGDVVDEQREVGD 269
              N    N +VNE +  +  E+      E     S  S      + D  RE+ D
Sbjct: 247 DLENSRSRNETVNEGSTPKPPEMQDSRDGEERRSASLNSGGGVVSIADILREIED 294

[Close]

BLAST of Lsi08G009890 vs. ExPASy Swiss-Prot
Match: Q7XLY8 (E3 ubiquitin-protein ligase Os04g0590900 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0590900 PE=2 SV=2)

HSP 1 Score: 120.6 bits (301), Expect = 3.2e-26
Identity = 89/277 (32.13%), Postives = 129/277 (46.57%), Query Frame = 0


Query: 18  QPNTNCSS--CPYDCPFDCYGIPDFYFSPPPP-PPPPTLTDHQKQ---LPTIIIILILFV 77
           +P  +CS   C   CP  CY    F F PPPP     T  D        P +I I+ +  
Sbjct: 12  EPTRDCSQGLCSMYCPQWCY----FIFPPPPPFDVAGTSADDSSGPVFSPLVIAIIGVLA 71

Query: 78  GFFIAFSFYIIVVK-CRSWQS------GSGNEGTEALQSDGGEGEFMNENQIDHPIWFIT 137
             F+  S+Y  + K C +  S      GS + G       G  G   +     H  W ++
Sbjct: 72  SAFLLVSYYTFISKYCGTVSSLRGRVFGSSSGGAAYGGGAGSGGRHGHGQSRSHESWNVS 131

Query: 138 -TVGLQQSVINSITVCKYKKSEGLIEGTDCSVCLSEFQEDEMLRLLPKCSHAFHIGCVDT 197
              GL +++IN ITVCKY++ +G +  TDCSVCL EF + E LRLLP+CSHAFH  C+DT
Sbjct: 132 PPSGLDETLINKITVCKYRRGDGFVHTTDCSVCLGEFSDGESLRLLPRCSHAFHQQCIDT 191

Query: 198 WLRTHTTCPLCRAHILTDFTNSNSARPPNLGPVNQNEANLSVNEETQMENDEINGEVLVE 257
           WL++H+ CPLCRA+I        S  P    P    E       E  +  D +      +
Sbjct: 192 WLKSHSNCPLCRANITFVTVGLASPEPEGCAP---GETGGDNTHEVVVVMDGLENLCEEQ 251

Query: 258 NEGVVSSGSSENRGDVVDEQREVGDLQIGEIVPKEGN 281
            E V  + ++++  D  D    + +      + +EG+
Sbjct: 252 QEAVSRASTADDDHDAKDVAEGMEEANGAAEIREEGS 281

[Close]

BLAST of Lsi08G009890 vs. ExPASy TrEMBL
Match: A0A5A7VDK2 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G004620 PE=3 SV=1)

HSP 1 Score: 506.9 bits (1304), Expect = 5.8e-140
Identity = 262/311 (84.24%), Postives = 275/311 (88.42%), Query Frame = 0


Query: 1   MAVTISRKLFPVATGTTQPNTNCSSCPYDCPFDCYGIPDFYFSPPPPPPPPTLTDHQKQL 60
           MAVT SRKLFPVATGTTQP TNCS+CPYDCP+DCYG PD YFSPPPPPPP  L DHQKQL
Sbjct: 1   MAVTHSRKLFPVATGTTQPTTNCSACPYDCPYDCYGFPDLYFSPPPPPPP--LFDHQKQL 60

Query: 61  PTIIIILILFVGFFIAFSFYIIVVKCRSWQSGSGNEGTEALQSDGGEGEFMNENQIDHPI 120
           PT+II+LILFV FFIAF+FYIIVVKCRSW SGSGNEG EALQSDGGEGEFMNENQ+DHPI
Sbjct: 61  PTMIIVLILFVAFFIAFTFYIIVVKCRSWYSGSGNEGAEALQSDGGEGEFMNENQVDHPI 120

Query: 121 WFITTVGLQQSVINSITVCKYKKSEGLIEGTDCSVCLSEFQEDEMLRLLPKCSHAFHIGC 180
           WFITT GLQQSVINSITVCKYKKSEGLIEGTDCSVCLSEFQEDEMLRLLPKCSHAFHIGC
Sbjct: 121 WFITTAGLQQSVINSITVCKYKKSEGLIEGTDCSVCLSEFQEDEMLRLLPKCSHAFHIGC 180

Query: 181 VDTWLRTHTTCPLCRAHILTDFT--NSNSARPPNLGPVNQNEANLSVNEETQMENDEING 240
           VDTWLRTHTTCPLCRAHILTDFT  NSNS RPPNLGP+NQNE NL +NE+TQMEN+ ING
Sbjct: 181 VDTWLRTHTTCPLCRAHILTDFTTSNSNSIRPPNLGPLNQNEGNLGLNEDTQMENENING 240

Query: 241 EVLVENEG---VVSSGSSENRGDVVDEQREVGDLQIGEIVPKEGNFESDESKVEGNESMR 300
           E + ENEG    +SS SSENRGDVVDEQREV      EIV KEGNFESD+SKVEGNES R
Sbjct: 241 EAVRENEGGVVSISSESSENRGDVVDEQREV-----EEIVQKEGNFESDDSKVEGNESTR 300

Query: 301 N-NEMDSSSAT 306
           N N M SSS T
Sbjct: 301 NDNRMYSSSTT 304

[Close]

BLAST of Lsi08G009890 vs. ExPASy TrEMBL
Match: A0A1S3BAW8 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103488086 PE=3 SV=1)

HSP 1 Score: 506.9 bits (1304), Expect = 5.8e-140
Identity = 262/311 (84.24%), Postives = 275/311 (88.42%), Query Frame = 0


Query: 1   MAVTISRKLFPVATGTTQPNTNCSSCPYDCPFDCYGIPDFYFSPPPPPPPPTLTDHQKQL 60
           MAVT SRKLFPVATGTTQP TNCS+CPYDCP+DCYG PD YFSPPPPPPP  L DHQKQL
Sbjct: 1   MAVTHSRKLFPVATGTTQPTTNCSACPYDCPYDCYGFPDLYFSPPPPPPP--LFDHQKQL 60

Query: 61  PTIIIILILFVGFFIAFSFYIIVVKCRSWQSGSGNEGTEALQSDGGEGEFMNENQIDHPI 120
           PT+II+LILFV FFIAF+FYIIVVKCRSW SGSGNEG EALQSDGGEGEFMNENQ+DHPI
Sbjct: 61  PTMIIVLILFVAFFIAFTFYIIVVKCRSWYSGSGNEGAEALQSDGGEGEFMNENQVDHPI 120

Query: 121 WFITTVGLQQSVINSITVCKYKKSEGLIEGTDCSVCLSEFQEDEMLRLLPKCSHAFHIGC 180
           WFITT GLQQSVINSITVCKYKKSEGLIEGTDCSVCLSEFQEDEMLRLLPKCSHAFHIGC
Sbjct: 121 WFITTAGLQQSVINSITVCKYKKSEGLIEGTDCSVCLSEFQEDEMLRLLPKCSHAFHIGC 180

Query: 181 VDTWLRTHTTCPLCRAHILTDFT--NSNSARPPNLGPVNQNEANLSVNEETQMENDEING 240
           VDTWLRTHTTCPLCRAHILTDFT  NSNS RPPNLGP+NQNE NL +NE+TQMEN+ ING
Sbjct: 181 VDTWLRTHTTCPLCRAHILTDFTTSNSNSIRPPNLGPLNQNEGNLGLNEDTQMENENING 240

Query: 241 EVLVENEG---VVSSGSSENRGDVVDEQREVGDLQIGEIVPKEGNFESDESKVEGNESMR 300
           E + ENEG    +SS SSENRGDVVDEQREV      EIV KEGNFESD+SKVEGNES R
Sbjct: 241 EAVRENEGGVVSISSESSENRGDVVDEQREV-----EEIVQKEGNFESDDSKVEGNESTR 300

Query: 301 N-NEMDSSSAT 306
           N N M SSS T
Sbjct: 301 NDNRMYSSSTT 304

[Close]

BLAST of Lsi08G009890 vs. ExPASy TrEMBL
Match: A0A0A0LPJ4 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_2G369190 PE=3 SV=1)

HSP 1 Score: 500.4 bits (1287), Expect = 5.5e-138
Identity = 253/309 (81.88%), Postives = 271/309 (87.70%), Query Frame = 0


Query: 1   MAVTISRKLFPVATGTTQPNTNCSSCPYDCPFDCYGIPDFYFSPPPPPPPPTLTDHQKQL 60
           MAVT+SRKLFPVATGT  P TNCS+CPYDCP+DCYG PD YFSPPPPPPP  L+DHQKQL
Sbjct: 1   MAVTLSRKLFPVATGTALPTTNCSACPYDCPYDCYGFPDLYFSPPPPPPP--LSDHQKQL 60

Query: 61  PTIIIILILFVGFFIAFSFYIIVVKCRSWQSGSGNEGTEALQSDGGEGEFMNENQIDHPI 120
           PT IIILILF+ FF+AF+FYIIVVKCRSW SGS NEG EALQSDGGEGEFMNENQ+DHPI
Sbjct: 61  PTTIIILILFIAFFVAFTFYIIVVKCRSWYSGSANEGAEALQSDGGEGEFMNENQVDHPI 120

Query: 121 WFITTVGLQQSVINSITVCKYKKSEGLIEGTDCSVCLSEFQEDEMLRLLPKCSHAFHIGC 180
           WFITT GLQQSVINSITVCKYKKSEGLIEGTDCSVCLSEFQEDEMLRLLPKCSHAFHIGC
Sbjct: 121 WFITTAGLQQSVINSITVCKYKKSEGLIEGTDCSVCLSEFQEDEMLRLLPKCSHAFHIGC 180

Query: 181 VDTWLRTHTTCPLCRAHILTDFTNSNSARPPNLGPVNQNEANLSVNEETQMENDEINGEV 240
           VDTWLRTHTTCPLCRAHILTDFT  NS RPPN+GP+NQNE NL +NE+TQMEN+  N E 
Sbjct: 181 VDTWLRTHTTCPLCRAHILTDFTTPNSVRPPNIGPLNQNEGNLGLNEDTQMENENTNREA 240

Query: 241 LVENEG---VVSSGSSENRGDVVDEQREVGDLQIGEIVPKEGNFESDESKVEGNESMRN- 300
           + ENEG    +SS SSENRGD VDEQREV      EIV KEGNFESD+SKVEGNESMRN 
Sbjct: 241 VRENEGGGVSISSESSENRGDAVDEQREV-----EEIVQKEGNFESDDSKVEGNESMRND 300

Query: 301 NEMDSSSAT 306
           N+M+SSS T
Sbjct: 301 NQMNSSSTT 302

[Close]

BLAST of Lsi08G009890 vs. ExPASy TrEMBL
Match: A0A6J1HEB6 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111462708 PE=3 SV=1)

HSP 1 Score: 439.5 bits (1129), Expect = 1.1e-119
Identity = 227/306 (74.18%), Postives = 252/306 (82.35%), Query Frame = 0


Query: 1   MAVTISRKLFPVATGTTQPNTNCSSCPYDCPFDCYGIPDFYFSPPPPPPPPTLTDHQKQL 60
           MAV I+RKLFPVA GT Q + NCS+CPYDCPFDC  +PDFYFSPPPP P P   DHQKQL
Sbjct: 1   MAVGITRKLFPVAAGTHQQSFNCSACPYDCPFDCLDLPDFYFSPPPPLPLPPFGDHQKQL 60

Query: 61  PTI-IIILILFVGFFIAFSFYIIVVKCRSWQSGSGNEGTEALQSDGGEGEFMNENQIDHP 120
           PTI I+IL LFVGFF  FSFYII+VKC++W+SGSGN+G EA Q +G +GEF+NENQ+DHP
Sbjct: 61  PTIVIVILALFVGFFFTFSFYIIIVKCQAWRSGSGNQGAEASQLNGEDGEFLNENQVDHP 120

Query: 121 IWFITTVGLQQSVINSITVCKYKKSEGLIEGTDCSVCLSEFQEDEMLRLLPKCSHAFHIG 180
           IWFITTVGLQQS+INSITVCKYKK EGLIEGT CSVCL+EFQEDEMLRLLPKCSHAFHIG
Sbjct: 121 IWFITTVGLQQSIINSITVCKYKKGEGLIEGTVCSVCLNEFQEDEMLRLLPKCSHAFHIG 180

Query: 181 CVDTWLRTHTTCPLCRAHILTDFTNSNSARPPNLGPVNQNEANLSVNEETQMENDEINGE 240
           CVDTWLRTHTTCPLCRAHILTDF  SN+ARPPN G +NQNEANL V EETQMEN  + GE
Sbjct: 181 CVDTWLRTHTTCPLCRAHILTDFPTSNAARPPNSGHLNQNEANLGVIEETQMEN--LEGE 240

Query: 241 VLVENEGVVSSGSSENRGDVVDEQREVGDLQIGEIVPKEGNFESDESKVEGNESMRNNEM 300
              E   V   G  ENRGD V EQR++G  QIGEIVPKEG FESD+SK+E  E+M    M
Sbjct: 241 ---EQSAVSGGGGVENRGDAVYEQRDIGVPQIGEIVPKEGVFESDDSKMEVTETM----M 297

Query: 301 DSSSAT 306
           + SSAT
Sbjct: 301 NCSSAT 297

[Close]

BLAST of Lsi08G009890 vs. ExPASy TrEMBL
Match: A0A6J1K4G1 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111492196 PE=3 SV=1)

HSP 1 Score: 434.9 bits (1117), Expect = 2.8e-118
Identity = 228/306 (74.51%), Postives = 249/306 (81.37%), Query Frame = 0


Query: 1   MAVTISRKLFPVATGTTQPNTNCSSCPYDCPFDCYGIPDFYFSPPPPPPPPTLTDHQKQL 60
           MAV I+RKLFPV TGT Q + NCS CPYDCPFDC  +PDFYFSPPPP       DHQKQL
Sbjct: 1   MAVGITRKLFPVETGTHQQSFNCSGCPYDCPFDCLDLPDFYFSPPPP-----FGDHQKQL 60

Query: 61  PTI-IIILILFVGFFIAFSFYIIVVKCRSWQSGSGNEGTEALQSDGGEGEFMNENQIDHP 120
           PTI I+IL LFVGFF  FSFYII+VK ++W+SGSGN+G EA Q DG +GEF+NENQ+DHP
Sbjct: 61  PTIVIVILALFVGFFFTFSFYIIIVKWQAWRSGSGNQGAEASQLDGEDGEFLNENQVDHP 120

Query: 121 IWFITTVGLQQSVINSITVCKYKKSEGLIEGTDCSVCLSEFQEDEMLRLLPKCSHAFHIG 180
           IWFITTVGLQQS+INSITVCKYKK EGLIEGT CSVCL+EFQEDEMLRLLPKCSHAFHIG
Sbjct: 121 IWFITTVGLQQSIINSITVCKYKKGEGLIEGTVCSVCLNEFQEDEMLRLLPKCSHAFHIG 180

Query: 181 CVDTWLRTHTTCPLCRAHILTDFTNSNSARPPNLGPVNQNEANLSVNEETQMENDEINGE 240
           CVDTWLRTHTTCPLCRAHILTDF  SN  RPPN G VNQNEANL+V EETQMEN  + GE
Sbjct: 181 CVDTWLRTHTTCPLCRAHILTDFATSNFPRPPNSGHVNQNEANLAVIEETQMEN--LEGE 240

Query: 241 VLVENEGVVSSGSSENRGDVVDEQREVGDLQIGEIVPKEGNFESDESKVEGNESMRNNEM 300
              E   V   G  ENRGD VDEQR+VG  QIGEIVPKEG FESD+SK+E NE+M    M
Sbjct: 241 ---EQSAVSGGGGVENRGDAVDEQRDVGVPQIGEIVPKEGVFESDDSKMEVNETM----M 292

Query: 301 DSSSAT 306
           + SSAT
Sbjct: 301 NCSSAT 292

[Close]

BLAST of Lsi08G009890 vs. NCBI nr
Match: XP_038884462.1 (RING-H2 finger protein ATL54-like [Benincasa hispida])

HSP 1 Score: 531.6 bits (1368), Expect = 4.6e-147
Identity = 268/305 (87.87%), Postives = 279/305 (91.48%), Query Frame = 0


Query: 1   MAVTISRKLFPVATGTTQPNTNCSSCPYDCPFDCYGIPDFYFSPPPPPPPPTLTDHQKQL 60
           MAVTISRKLFPVATGTTQP TNCS CPYDCPFDCYGIPDFY+S PPPPPP  L DHQKQL
Sbjct: 1   MAVTISRKLFPVATGTTQPTTNCSGCPYDCPFDCYGIPDFYYSQPPPPPP--LADHQKQL 60

Query: 61  PTIIIILILFVGFFIAFSFYIIVVKCRSWQSGSGNEGTEALQSDGGEGEFMNENQIDHPI 120
           PT+IIIL+LFVGFFIAFSFYIIVVKCRSWQSGSGNEGTEALQSDGGEGEFMNENQ+DHPI
Sbjct: 61  PTLIIILLLFVGFFIAFSFYIIVVKCRSWQSGSGNEGTEALQSDGGEGEFMNENQVDHPI 120

Query: 121 WFITTVGLQQSVINSITVCKYKKSEGLIEGTDCSVCLSEFQEDEMLRLLPKCSHAFHIGC 180
           WFITT GLQQSVINSITVCKYKKSEGLIEGT+CSVCLSEFQEDEMLRLLPKCSHAFHIGC
Sbjct: 121 WFITTAGLQQSVINSITVCKYKKSEGLIEGTECSVCLSEFQEDEMLRLLPKCSHAFHIGC 180

Query: 181 VDTWLRTHTTCPLCRAHILTDFTNSNSARPPNLGPVNQNEANLSVNEETQMENDEINGEV 240
           VDTWLRTHTTCPLCRAHILTDFT SNS RPPNLGPVNQNEANL VNEETQMEN+EINGEV
Sbjct: 181 VDTWLRTHTTCPLCRAHILTDFTASNSVRPPNLGPVNQNEANLGVNEETQMENEEINGEV 240

Query: 241 LVENEGVVSSGSSENRGDVVDEQREVGDLQIGEIVPKEGNFESDESKVEGNESMRNNEMD 300
           LVENEGVVS G+ ENRGDVVDEQREVG +Q            +D+SKVEGNES RNN+MD
Sbjct: 241 LVENEGVVSRGNFENRGDVVDEQREVGIVQ------------TDDSKVEGNESTRNNQMD 291

Query: 301 SSSAT 306
           SSSAT
Sbjct: 301 SSSAT 291

[Close]

BLAST of Lsi08G009890 vs. NCBI nr
Match: XP_008444871.1 (PREDICTED: RING-H2 finger protein ATL54-like [Cucumis melo] >KAA0065127.1 RING-H2 finger protein ATL54-like [Cucumis melo var. makuwa])

HSP 1 Score: 506.9 bits (1304), Expect = 1.2e-139
Identity = 262/311 (84.24%), Postives = 275/311 (88.42%), Query Frame = 0


Query: 1   MAVTISRKLFPVATGTTQPNTNCSSCPYDCPFDCYGIPDFYFSPPPPPPPPTLTDHQKQL 60
           MAVT SRKLFPVATGTTQP TNCS+CPYDCP+DCYG PD YFSPPPPPPP  L DHQKQL
Sbjct: 1   MAVTHSRKLFPVATGTTQPTTNCSACPYDCPYDCYGFPDLYFSPPPPPPP--LFDHQKQL 60

Query: 61  PTIIIILILFVGFFIAFSFYIIVVKCRSWQSGSGNEGTEALQSDGGEGEFMNENQIDHPI 120
           PT+II+LILFV FFIAF+FYIIVVKCRSW SGSGNEG EALQSDGGEGEFMNENQ+DHPI
Sbjct: 61  PTMIIVLILFVAFFIAFTFYIIVVKCRSWYSGSGNEGAEALQSDGGEGEFMNENQVDHPI 120

Query: 121 WFITTVGLQQSVINSITVCKYKKSEGLIEGTDCSVCLSEFQEDEMLRLLPKCSHAFHIGC 180
           WFITT GLQQSVINSITVCKYKKSEGLIEGTDCSVCLSEFQEDEMLRLLPKCSHAFHIGC
Sbjct: 121 WFITTAGLQQSVINSITVCKYKKSEGLIEGTDCSVCLSEFQEDEMLRLLPKCSHAFHIGC 180

Query: 181 VDTWLRTHTTCPLCRAHILTDFT--NSNSARPPNLGPVNQNEANLSVNEETQMENDEING 240
           VDTWLRTHTTCPLCRAHILTDFT  NSNS RPPNLGP+NQNE NL +NE+TQMEN+ ING
Sbjct: 181 VDTWLRTHTTCPLCRAHILTDFTTSNSNSIRPPNLGPLNQNEGNLGLNEDTQMENENING 240

Query: 241 EVLVENEG---VVSSGSSENRGDVVDEQREVGDLQIGEIVPKEGNFESDESKVEGNESMR 300
           E + ENEG    +SS SSENRGDVVDEQREV      EIV KEGNFESD+SKVEGNES R
Sbjct: 241 EAVRENEGGVVSISSESSENRGDVVDEQREV-----EEIVQKEGNFESDDSKVEGNESTR 300

Query: 301 N-NEMDSSSAT 306
           N N M SSS T
Sbjct: 301 NDNRMYSSSTT 304

[Close]

BLAST of Lsi08G009890 vs. NCBI nr
Match: XP_011649673.1 (RING-H2 finger protein ATL54 [Cucumis sativus] >KGN62717.1 hypothetical protein Csa_022380 [Cucumis sativus])

HSP 1 Score: 500.4 bits (1287), Expect = 1.1e-137
Identity = 253/309 (81.88%), Postives = 271/309 (87.70%), Query Frame = 0


Query: 1   MAVTISRKLFPVATGTTQPNTNCSSCPYDCPFDCYGIPDFYFSPPPPPPPPTLTDHQKQL 60
           MAVT+SRKLFPVATGT  P TNCS+CPYDCP+DCYG PD YFSPPPPPPP  L+DHQKQL
Sbjct: 1   MAVTLSRKLFPVATGTALPTTNCSACPYDCPYDCYGFPDLYFSPPPPPPP--LSDHQKQL 60

Query: 61  PTIIIILILFVGFFIAFSFYIIVVKCRSWQSGSGNEGTEALQSDGGEGEFMNENQIDHPI 120
           PT IIILILF+ FF+AF+FYIIVVKCRSW SGS NEG EALQSDGGEGEFMNENQ+DHPI
Sbjct: 61  PTTIIILILFIAFFVAFTFYIIVVKCRSWYSGSANEGAEALQSDGGEGEFMNENQVDHPI 120

Query: 121 WFITTVGLQQSVINSITVCKYKKSEGLIEGTDCSVCLSEFQEDEMLRLLPKCSHAFHIGC 180
           WFITT GLQQSVINSITVCKYKKSEGLIEGTDCSVCLSEFQEDEMLRLLPKCSHAFHIGC
Sbjct: 121 WFITTAGLQQSVINSITVCKYKKSEGLIEGTDCSVCLSEFQEDEMLRLLPKCSHAFHIGC 180

Query: 181 VDTWLRTHTTCPLCRAHILTDFTNSNSARPPNLGPVNQNEANLSVNEETQMENDEINGEV 240
           VDTWLRTHTTCPLCRAHILTDFT  NS RPPN+GP+NQNE NL +NE+TQMEN+  N E 
Sbjct: 181 VDTWLRTHTTCPLCRAHILTDFTTPNSVRPPNIGPLNQNEGNLGLNEDTQMENENTNREA 240

Query: 241 LVENEG---VVSSGSSENRGDVVDEQREVGDLQIGEIVPKEGNFESDESKVEGNESMRN- 300
           + ENEG    +SS SSENRGD VDEQREV      EIV KEGNFESD+SKVEGNESMRN 
Sbjct: 241 VRENEGGGVSISSESSENRGDAVDEQREV-----EEIVQKEGNFESDDSKVEGNESMRND 300

Query: 301 NEMDSSSAT 306
           N+M+SSS T
Sbjct: 301 NQMNSSSTT 302

[Close]

BLAST of Lsi08G009890 vs. NCBI nr
Match: XP_023546737.1 (RING-H2 finger protein ATL54-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 442.6 bits (1137), Expect = 2.8e-120
Identity = 229/306 (74.84%), Postives = 253/306 (82.68%), Query Frame = 0


Query: 1   MAVTISRKLFPVATGTTQPNTNCSSCPYDCPFDCYGIPDFYFSPPPPPPPPTLTDHQKQL 60
           MAV I+RKLFPVATGT Q + NCS+CPYDCP DC  +PDFYFSPPPPPPP    DHQKQL
Sbjct: 1   MAVGITRKLFPVATGTHQQSFNCSACPYDCPLDCLDLPDFYFSPPPPPPP--FDDHQKQL 60

Query: 61  PTI-IIILILFVGFFIAFSFYIIVVKCRSWQSGSGNEGTEALQSDGGEGEFMNENQIDHP 120
           PTI I+IL LFVGFF  FSFYIIVVKC++W+SGSGN+G E  Q DG +GEF+NENQ+DHP
Sbjct: 61  PTIVIVILALFVGFFFTFSFYIIVVKCQAWRSGSGNQGAETSQLDGEDGEFLNENQVDHP 120

Query: 121 IWFITTVGLQQSVINSITVCKYKKSEGLIEGTDCSVCLSEFQEDEMLRLLPKCSHAFHIG 180
           IWFITTVGLQQS+INSITVCKYKK EGLIEGT CSVCL+EFQEDE+LRLLPKCSHAFHIG
Sbjct: 121 IWFITTVGLQQSIINSITVCKYKKGEGLIEGTVCSVCLNEFQEDELLRLLPKCSHAFHIG 180

Query: 181 CVDTWLRTHTTCPLCRAHILTDFTNSNSARPPNLGPVNQNEANLSVNEETQMENDEINGE 240
           CVDTWLRTHTTCPLCRAHILTDF  SN+ARPPN G +NQNEANL V EETQMEN  + GE
Sbjct: 181 CVDTWLRTHTTCPLCRAHILTDFPTSNTARPPNSGHLNQNEANLVVIEETQMEN--LEGE 240

Query: 241 VLVENEGVVSSGSSENRGDVVDEQREVGDLQIGEIVPKEGNFESDESKVEGNESMRNNEM 300
              E   V   G  ENRGD VDEQR++G   IGEIVPKEG FESD+SK+E NE+M    M
Sbjct: 241 ---EQSAVSGGGGVENRGDAVDEQRDIGVPPIGEIVPKEGVFESDDSKMEVNETM----M 295

Query: 301 DSSSAT 306
           + SSAT
Sbjct: 301 NCSSAT 295

[Close]

BLAST of Lsi08G009890 vs. NCBI nr
Match: KAG6598705.1 (RING-H2 finger protein ATL54, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 441.0 bits (1133), Expect = 8.1e-120
Identity = 226/306 (73.86%), Postives = 252/306 (82.35%), Query Frame = 0


Query: 1   MAVTISRKLFPVATGTTQPNTNCSSCPYDCPFDCYGIPDFYFSPPPPPPPPTLTDHQKQL 60
           MAV I+RKLFPVA GT Q + NCS+CPYDCPFDC  +PDFYFSPPPPPP P   DHQKQL
Sbjct: 1   MAVGITRKLFPVAAGTHQQSFNCSACPYDCPFDCLDLPDFYFSPPPPPPLPPFGDHQKQL 60

Query: 61  PTI-IIILILFVGFFIAFSFYIIVVKCRSWQSGSGNEGTEALQSDGGEGEFMNENQIDHP 120
           PTI I+IL LFVGFF  FSFYII+VKC++W+SGSGN+G EA Q +G +GEF+NENQ+DHP
Sbjct: 61  PTIVIVILALFVGFFFTFSFYIIIVKCQAWRSGSGNQGAEASQLNGEDGEFLNENQVDHP 120

Query: 121 IWFITTVGLQQSVINSITVCKYKKSEGLIEGTDCSVCLSEFQEDEMLRLLPKCSHAFHIG 180
           IWFITTVGLQQS+INSITVCKYKK EGLIEGT CSVCL+EFQEDEMLRLLPKCSHAFHIG
Sbjct: 121 IWFITTVGLQQSIINSITVCKYKKGEGLIEGTVCSVCLNEFQEDEMLRLLPKCSHAFHIG 180

Query: 181 CVDTWLRTHTTCPLCRAHILTDFTNSNSARPPNLGPVNQNEANLSVNEETQMENDEINGE 240
           CVDTWLRTHTTCPLCRAHILTDF  SN+ARPPN G +NQNEANL V +ETQMEN  + GE
Sbjct: 181 CVDTWLRTHTTCPLCRAHILTDFPTSNAARPPNSGHLNQNEANLGVIQETQMEN--LEGE 240

Query: 241 VLVENEGVVSSGSSENRGDVVDEQREVGDLQIGEIVPKEGNFESDESKVEGNESMRNNEM 300
              E   V   G  ENRGD VDEQR++G  QIGEI PKEG FESD+SK+E  E+     M
Sbjct: 241 ---EQSAVSGGGGVENRGDAVDEQRDIGVPQIGEIAPKEGVFESDDSKMEVTET----TM 297

Query: 301 DSSSAT 306
           + SSAT
Sbjct: 301 NCSSAT 297

[Close]

BLAST of Lsi08G009890 vs. TAIR 10
Match: AT1G72220.1 (RING/U-box superfamily protein )

HSP 1 Score: 174.5 bits (441), Expect = 1.3e-43
Identity = 124/319 (38.87%), Postives = 163/319 (51.10%), Query Frame = 0


Query: 1   MAVTISRKLFPVATGTTQPNTNCSS--CPYDCPFDCYGIPDFY-FSP----------PPP 60
           MA    RKLFP     T    +CS+  C   CP++CY  PD+Y  SP          P P
Sbjct: 1   MARKKHRKLFPTLASETNKTLDCSNGVCDPICPYNCYPEPDYYTISPQLPPWSSSPQPSP 60

Query: 61  PPPPTLT-------DHQKQLPTIIIILILFVGFFIAFSFYIIVVKCRSWQSGSGNEGTEA 120
            P P+++       D    L  I II I      I  + + +V K  S      N+GT  
Sbjct: 61  CPSPSISAVYLPSQDSSSSLDAISIITITGAVLAILLTGFFLVAKFFSDSVNRVNQGTYQ 120

Query: 121 LQSDGG----EGEFMNENQIDHPIWFITTVGLQQSVINSITVCKYKKSEGLIEGTDCSVC 180
             ++      E EF +  Q+DHPIW I T GLQQS+INSIT+C YK+ +GLIE TDC VC
Sbjct: 121 SDNEDNDTVMEEEFQDREQVDHPIWLIRTTGLQQSIINSITICNYKRGDGLIERTDCPVC 180

Query: 181 LSEFQEDEMLRLLPKCSHAFHIGCVDTWLRTHTTCPLCRAHILTDFTNSNSARPPNLGPV 240
           L+EF+EDE LRLLPKC+HAFHI C+DTWL +HT CPLCRA I       +   P   GPV
Sbjct: 181 LNEFEEDESLRLLPKCNHAFHISCIDTWLSSHTNCPLCRAGI----AMISVTTPRYSGPV 240

Query: 241 NQNEANLSVNEETQMENDEINGEVLVENEGVVSSGSSENRGDVVDEQREVGDLQIGEIVP 296
                       + +END ++ E           G  ENR D   ++ +  D++I EI  
Sbjct: 241 EVTPG----GSGSHLENDGVDEE---------DHGEIENRVDSDFKESDDSDIRI-EIYR 299

[Close]

BLAST of Lsi08G009890 vs. TAIR 10
Match: AT5G10380.1 (RING/U-box superfamily protein )

HSP 1 Score: 153.3 bits (386), Expect = 3.2e-37
Identity = 81/159 (50.94%), Postives = 104/159 (65.41%), Query Frame = 0


Query: 44  PPPPPPPPTLTDHQKQLPTIIII---LILFVGFFIAFSFYIIVVKCRSWQSGSGNEGTEA 103
           PPPPPPPP   +    LPT I +   +ILF+  F+ F  Y+ + + R   + S   G   
Sbjct: 26  PPPPPPPPPPRELSLLLPTSICVVGSIILFL--FLVFFLYLHITQQRRISAASVTPGDTN 85

Query: 104 LQSDGGEGEFMNENQIDHPIWFITTVGLQQSVINSITVCKYKKSEGLIEGTDCSVCLSEF 163
            Q D  E E  + +   H +W I TVGL +S INSITV  +KK EG+I+GT+CSVCL+EF
Sbjct: 86  QQEDEDETEERDFSDFHH-VWQIPTVGLHRSAINSITVVGFKKGEGIIDGTECSVCLNEF 145

Query: 164 QEDEMLRLLPKCSHAFHIGCVDTWLRTHTTCPLCRAHIL 200
           +EDE LRLLPKCSHAFH+ C+DTWL +H  CPLCRA +L
Sbjct: 146 EEDESLRLLPKCSHAFHLNCIDTWLLSHKNCPLCRAPVL 181

[Close]

BLAST of Lsi08G009890 vs. TAIR 10
Match: AT4G33565.1 (RING/U-box superfamily protein )

HSP 1 Score: 136.0 bits (341), Expect = 5.2e-32
Identity = 95/246 (38.62%), Postives = 124/246 (50.41%), Query Frame = 0


Query: 23  CSS-CPYDCPFDCYGIPDFYFSPPPPPPPPTLTDHQKQLPTIIII--------LILFVGF 82
           CSS C  +CP  C  +     SPP          H   L  I+II        L L    
Sbjct: 89  CSSYCLDECPSICEIVVISGLSPPAE------IHHDDNLKRILIISASSIITTLFLLTLL 148

Query: 83  FIAFSFYI----IVVKCRSWQSGSGNEGTEALQSDGGEGEFMNENQIDHPIWFITTVGLQ 142
            + F FY      +   R W      E       DG          +DHPIW I T+GL 
Sbjct: 149 VLCFKFYYRRRRSITTSRRWSM----EEARNWDFDGPAPVI-----VDHPIWHIRTIGLN 208

Query: 143 QSVINSITVCKYKKSEGLIEGTDCSVCLSEFQEDEMLRLLPKCSHAFHIGCVDTWLRTHT 202
            +VI+SI VC+Y K +G++EGTDCSVCLSEF+E+E LRLLPKC HAFH+ C+DTWLR+HT
Sbjct: 209 PTVISSIKVCQYSKKDGVVEGTDCSVCLSEFEEEETLRLLPKCKHAFHLYCIDTWLRSHT 268

Query: 203 TCPLCRAHILTDFTNSNSARPPNLGPVNQNEANLSVNEETQMENDEINGEVLVENEGVVS 256
            CPLCRA I+   T  +             E ++ + EE   + DE   E+  E +G VS
Sbjct: 269 NCPLCRAPIVEANTMIDDHSE------GLEEISVMIPEENGDDTDE---EIEEERDGFVS 310

[Close]

BLAST of Lsi08G009890 vs. TAIR 10
Match: AT5G17600.1 (RING/U-box superfamily protein )

HSP 1 Score: 122.5 bits (306), Expect = 6.0e-28
Identity = 93/267 (34.83%), Postives = 139/267 (52.06%), Query Frame = 0


Query: 27  PYDCPFDC-YGIPDFYFSP------PPPPPPPTLTDHQKQ-----LPTIIIILILFVGFF 86
           PYD   DC  GI + Y         PPPPP   L D          P +I ++ +     
Sbjct: 10  PYDSYNDCSQGICNIYCPQWCYLIFPPPPPSFFLDDDSSSSSSSFSPLLIALIGILTSAL 69

Query: 87  IAFSFYIIVVK-CRSWQSGSGNEGTEALQSDGGEGEFMNENQIDHPIWFITTVGLQQSVI 146
           I  S+Y ++ K C      S +E      +  GEG F +  +I          GL +S+I
Sbjct: 70  ILVSYYTLISKYCHRHHQTSSSETLNL--NHNGEGFFSSTQRIS-----TNGDGLNESMI 129

Query: 147 NSITVCKYKKSEGLIEGTDCSVCLSEFQEDEMLRLLPKCSHAFHIGCVDTWLRTHTTCPL 206
            SITV KYK  +G ++G+DCSVCLSEF+E+E LRLLPKC+HAFH+ C+DTWL++H+ CPL
Sbjct: 130 KSITVYKYKSGDGFVDGSDCSVCLSEFEENESLRLLPKCNHAFHLPCIDTWLKSHSNCPL 189

Query: 207 CRAHILTDFTNSNSARPPNLGPVNQNEANL-----SVNEET------QMENDEINGEVLV 266
           CRA + T   N  ++   N+  V  N++N      SV+E        + +  + +  V++
Sbjct: 190 CRAFV-TGVNNPTASVGQNVSVVVANQSNSAHQTGSVSEINLNLAGYESQTGDFDSVVVI 249

Query: 267 ENEGVVSSGS-SENRGDVVDEQREVGD 269
           E+  + S  S + +   + +E+RE  D
Sbjct: 250 EDLEIGSRNSDARSELQLPEERRETKD 268

[Close]

BLAST of Lsi08G009890 vs. TAIR 10
Match: AT3G03550.1 (RING/U-box superfamily protein )

HSP 1 Score: 121.3 bits (303), Expect = 1.3e-27
Identity = 101/295 (34.24%), Postives = 137/295 (46.44%), Query Frame = 0


Query: 11  PVATGTTQPN-TNCSS--CPYDCPFDCYGIPDFYFSPPPPPPPPTLTDHQKQ-----LPT 70
           P   GTT  +  +CS   C   CP  CY I        PPPP   L D          P 
Sbjct: 7   PNPWGTTYDSYRDCSQGVCSVYCPQWCYVI-------FPPPPSFYLDDEDDSSSSDFSPL 66

Query: 71  IIIILILFVGFFIAFSFYIIVVK-CRSWQSGSGNEGTEALQSDGGEGEFM-----NENQI 130
           +I ++ +    FI  S+Y ++ K C   +  S +    A+     +  +      N N  
Sbjct: 67  LIALIGILASAFILVSYYTLISKYCHRRRHNSSSTSAAAINRISSDYTWQGTNNNNNNGA 126

Query: 131 DHPIWFI---TTVGLQQSVINSITVCKYKKSEGLIEGTDCSVCLSEFQEDEMLRLLPKCS 190
            +P   I      GL +S+I SITV KY+K +G +E +DCSVCLSEFQE+E LRLLPKC+
Sbjct: 127 TNPNQTIGGGGGDGLDESLIKSITVYKYRKMDGFVESSDCSVCLSEFQENESLRLLPKCN 186

Query: 191 HAFHIGCVDTWLRTHTTCPLCRAHILT-------DFTN-------------SNSARPPNL 250
           HAFH+ C+DTWL++H+ CPLCRA I+T       D TN              +S    NL
Sbjct: 187 HAFHVPCIDTWLKSHSNCPLCRAFIVTSSAVEIVDLTNQQIVTENNSISTGDDSVVVVNL 246

Query: 251 GPVNQNEANLSVNEETQMENDEINGEVLVENEGVVSSGSSENRGDVVDEQREVGD 269
              N    N +VNE +  +  E+      E     S  S      + D  RE+ D
Sbjct: 247 DLENSRSRNETVNEGSTPKPPEMQDSRDGEERRSASLNSGGGVVSIADILREIED 294

The following BLAST results are available for this feature:

BLAST of Lsi08G009890 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LFY8	1.9e-42	38.87	RING-H2 finger protein ATL54 OS=Arabidopsis thaliana OX=3702 GN=ATL54 PE=2 SV=2	[more]
Q9LX93	4.4e-36	50.94	E3 ubiquitin-protein ligase RING1 OS=Arabidopsis thaliana OX=3702 GN=ATL55 PE=1 ...	[more]
Q9LF64	8.4e-27	34.83	RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1	[more]
Q9SRQ8	1.9e-26	34.24	RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2	[more]
Q7XLY8	3.2e-26	32.13	E3 ubiquitin-protein ligase Os04g0590900 OS=Oryza sativa subsp. japonica OX=3994...	[more]

BLAST of Lsi08G009890 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7VDK2	5.8e-140	84.24	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C...	[more]
A0A1S3BAW8	5.8e-140	84.24	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103488086 PE=3 ...	[more]
A0A0A0LPJ4	5.5e-138	81.88	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_2G369190 PE...	[more]
A0A6J1HEB6	1.1e-119	74.18	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111462708...	[more]
A0A6J1K4G1	2.8e-118	74.51	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111492196 P...	[more]

BLAST of Lsi08G009890 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038884462.1	4.6e-147	87.87	RING-H2 finger protein ATL54-like [Benincasa hispida]	[more]
XP_008444871.1	1.2e-139	84.24	PREDICTED: RING-H2 finger protein ATL54-like [Cucumis melo] >KAA0065127.1 RING-H...	[more]
XP_011649673.1	1.1e-137	81.88	RING-H2 finger protein ATL54 [Cucumis sativus] >KGN62717.1 hypothetical protein ...	[more]
XP_023546737.1	2.8e-120	74.84	RING-H2 finger protein ATL54-like [Cucurbita pepo subsp. pepo]	[more]
KAG6598705.1	8.1e-120	73.86	RING-H2 finger protein ATL54, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Lsi08G009890 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G72220.1	1.3e-43	38.87	RING/U-box superfamily protein	[more]
AT5G10380.1	3.2e-37	50.94	RING/U-box superfamily protein	[more]
AT4G33565.1	5.2e-32	38.62	RING/U-box superfamily protein	[more]
AT5G17600.1	6.0e-28	34.83	RING/U-box superfamily protein	[more]
AT3G03550.1	1.3e-27	34.24	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 153..194 e-value: 1.1E-6 score: 38.2
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 152..195 e-value: 2.6E-13 score: 50.1
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 153..195 score: 12.740079
None	No IPR available	SMART	SM01197	FANCL_C_2	coord: 149..203 e-value: 0.0097 score: -1.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 247..266
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 278..294
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 273..305
None	No IPR available	PANTHER	PTHR45676:SF121	TRANSCRIPTION FACTOR C2H2 FAMILY-RELATED	coord: 7..304
None	No IPR available	CDD	cd16454	RING-H2_PA-TM-RING	coord: 152..195 e-value: 7.43728E-19 score: 76.1865
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 143..202
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 127..202 e-value: 1.1E-21 score: 77.8
IPR044675	E3 ubiquitin-protein ligase RING1-like	PANTHER	PTHR45676	RING-H2 FINGER PROTEIN ATL51-RELATED	coord: 7..304

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi08G009890.1	Lsi08G009890.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0016021	integral component of membrane