Lsi08G005540 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi08G005540
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	WAT1-related protein
Location	chr08: 13722594 .. 13725594 (+)
RNA-Seq Expression	Lsi08G005540
Synteny	Lsi08G005540

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CATCTTCTTACATTTTCAACTACCAAACTGTTAGTTAAAGAAACTTTGAAAAAAAAAATTATTATTATTATGAAGAGCTTTGTTGGTTATGTAGAGGCCATGGAGGTCCACAAGCCCTATATTGCAATGTTATTTGTTCAATGTGTTTATTCTGGAATGGCTTTATTATCGAAAGCAGCCATTTCTCAAGGCATGAATCCGTCCGTGTTCGTCTTTTATCGTCAAGCTTTTGCTACCGTTGCCATGGCTCCTCTCGCGTTCTTACTCGAAAGGTTTGTTATTTTCATATCCATAATTAAAAAAAAGTTGGTGTAAATTTGATTTTTTTTAATTTAAAGTTGAGATTTGTATTGTTTGCAGAAAAAAGGCAGTTCCTTTATCTTTCAAGTTTCTTTCTAAAGTGTTCTTGGTTTCTCTAATTGGGTATGTATAATGTGAATTTGATATTATATTCTTAATTATATCTCAATATATATAATAAGCTTAATTAATTAAATTCATCATTAAAAATTAATGATTTTTTTCAGGATTACTTTGAGCTTAAATCTTTATTACGTAGCAATCAGCCACACATCAGCAACATTTGCAGCTGCCACTACCAACACCATCCCTGCCATTACCCTCCTTTTGGCTCTCCTTTTCAGGTTAAATTATTATATATATGTACGTTACGTTTCACTATCCAGATTGACAATCATTTAATTTTTGAAAATGGTAGATTATTTTTTTTTTTCTAATTCTTTTCGGCTTTCATAAAGTATCATTTGGATTCATATATGAATTCTAAAAAGAAATTTTAAAACTACTTCTTTTACGGAATTCATATGACTCAAATTATAAAATTTTCAAAAACTAAATGGGTTATCAAACGAATACTTTATTTTTATTGCAAAAAACTACCCCTAAAAGTATGGTTGTAGTTACAATTATGCCTTCAAACTTTTCATTGTAAAAATTGAGCCCTCAAACTTATATAAGTGTTAAAACCTGACCCTCAAACTTACATAATTGGATAATTATATAAATTTGAGAGTTCAATTTTTATCATTTTGGGGTCCAGTTTCTACAACTATATTTGTAAGTTTGATGGTATAATTGCAACTATTACGATACTTTAGGAATGGTTTTTGCAATTTGTCGTATTTTTTTTAATGTTTGTTATTAATTTAAGTGAGATATTTGTTTGAATTGAATCAGATATGAAAGCATTTGCATAAGAAAGGTTGAAGGGATGGCCAAGTTGGTGGGAGGAGTGATAGGTTTTTCAGGGGCTTTAGTGTTTGCTTTTGTGAAGGGTCCACCAATGAAATTCATGAATTGGCATCCACAAACAAATAATAATAATAATTCAAATTCATTTCAAGCTTATTCCACATTAGAATGGATTAAGGGTTCATTCACTATGCTTTCAGCCAACATTGCTTGGTCTTTATGGCTTGTTTTGCAGGTTCAATTCCTTTTCTACCCTCTTTTTTTTCAATTTTGTTTTAAGAAACTTTTTAAAAATAGAAAAATAAGAAAAACTATTTACACAAAATAGCAAATTTAGATAGTTGTGATAGACGATGATAAAAGTCTATCAGAATCTATCAGTGATAAAAATGATAGAAGTCTATCAATGTCTATAAGTGCTTTTTTGCTATTTTCTGTAAATAGTTTGATATTCTTTTTTATCTGAGAAAATTTTCCTTGTTTTAATGGGTTTTTCAAGTTTTGAAGAATTTTTTTTTTTTTTTTTTGAAGGGCTTAATTGTGAAGGAATATCCAGCAAAATTGAGAATTACAACTTTGCAGTGTTTCTTTAGTTTGATACAATCAGCTTTGTGGGCTTTGGTAATGGAGAGAAAGCCAAAAGCTTGGAAACTTGGATGGAATCTTCAACTCTTCTCTGTTGCCTATTGTGTAAATATCTCTCTTTTCTTTACGATAAAATAATCAACGCTTCGTTTGATAACAATTTCATCGTTGTAATTTATGCGTGTTTCTTCTAAAAAATAACGTTTCAAAAAAACTACTCATTTCAGTTTTCAAAATTTAGTGTGGTTTTTTAAATCGTTAGTAATAAGTATATTAAACTTATAGAACATGGGAGTTAGCGTTCATAAGCTTAAATTACAAAAATAAACAATTACTGAATGATTATCAAACGAGACCTAAATTTACTATAATATATCTATCCACTTAAACTTTTTAGGTTCACTAACTCTTGTTATAAGAAAATTTTGTGTTTATTATAAACTCTTATAACGTCACGTCACTACCTCTTGTTGCAATTTATATTAACTTAAACGATTTTGAGTCTGATAGTGATTTTTTGTTTTGTTTTGTTCTGTTAGGAAATAATAGTTTGATAGTGATTTGATAGTTAGCTTATTAACTAACCTAAATTTCTTAAGTTTTGGGCTTAATCATGATTTAACTTTTTTTTTTTTTTTAGTCAGTCTTATAATATGGTCATTACCTTCTTGTTGCAACTTTATATTAAAGCTTTTGAGTTTTGATAGGAAATTTTACAATAACCTATCGCCTTAGAACTTATTAGCTTAAATATTTAGTACTCTTAAGCCACTTTTGGTTCGATCACGATTTAATATGGTAAATTAAAGTAAGAAGAAAACTCTATAATGTTATTACCTAATCTCGGACAATGAACATCGACAATCTCACTTATAATTTATTAAACTTAAATTTTTTAGTCAGACAGTGATTTGATTATAAAGAATTGTTTAAAATTATAGGGTATAATCGTGACCGGAATGACGTATTGGCTACAAATATGGAGTGTGGAGAAGAAAGGGCCAGTTTTCACAGCTATGTTTACACCATTAGCTCTCATCATAACAGCAATCTTCTCAGCATTATTATGGAAGGAAACCCTTCATTGGGGAAGGTCAATTCCTTTATTTTATTATTATTATTACTTTTAAAAAATTACAAATCTAGCAAAGTCTATCACATTTTTTAAAAAAATATTGCTATATATTGAGTTATTATTCATAAA

mRNA sequence

CATCTTCTTACATTTTCAACTACCAAACTGTTAGTTAAAGAAACTTTGAAAAAAAAAATTATTATTATTATGAAGAGCTTTGTTGGTTATGTAGAGGCCATGGAGGTCCACAAGCCCTATATTGCAATGTTATTTGTTCAATGTGTTTATTCTGGAATGGCTTTATTATCGAAAGCAGCCATTTCTCAAGGCATGAATCCGTCCGTGTTCGTCTTTTATCGTCAAGCTTTTGCTACCGTTGCCATGGCTCCTCTCGCGTTCTTACTCGAAAGAAAAAAGGCAGTTCCTTTATCTTTCAAGTTTCTTTCTAAAGTGTTCTTGGTTTCTCTAATTGGGATTACTTTGAGCTTAAATCTTTATTACGTAGCAATCAGCCACACATCAGCAACATTTGCAGCTGCCACTACCAACACCATCCCTGCCATTACCCTCCTTTTGGCTCTCCTTTTCAGAAAGGTTGAAGGGATGGCCAAGTTGGTGGGAGGAGTGATAGGTTTTTCAGGGGCTTTAGTGTTTGCTTTTGTGAAGGGTCCACCAATGAAATTCATGAATTGGCATCCACAAACAAATAATAATAATAATTCAAATTCATTTCAAGCTTATTCCACATTAGAATGGATTAAGGGTTCATTCACTATGCTTTCAGCCAACATTGCTTGGTCTTTATGGCTTTGTTTCTTTAGTTTGATACAATCAGCTTTGTGGGCTTTGGTAATGGAGAGAAAGCCAAAAGCTTGGAAACTTGGATGGAATCTTCAACTCTTCTCTGTTGCCTATTGTTCTTATAATATGGGTATAATCGTGACCGGAATGACGTATTGGCTACAAATATGGAGTGTGGAGAAGAAAGGGCCAGTTTTCACAGCTATGTTTACACCATTAGCTCTCATCATAACAGCAATCTTCTCAGCATTATTATGGAAGGAAACCCTTCATTGGGGAAGGTCAATTCCTTTATTTTATTATTATTATTACTTTTAAAAAATTACAAATCTAGCAAAGTCTATCACATTTTTTAAAAAAATATTGCTATATATTGAGTTATTATTCATAAA

Coding sequence (CDS)

ATGAAGAGCTTTGTTGGTTATGTAGAGGCCATGGAGGTCCACAAGCCCTATATTGCAATGTTATTTGTTCAATGTGTTTATTCTGGAATGGCTTTATTATCGAAAGCAGCCATTTCTCAAGGCATGAATCCGTCCGTGTTCGTCTTTTATCGTCAAGCTTTTGCTACCGTTGCCATGGCTCCTCTCGCGTTCTTACTCGAAAGAAAAAAGGCAGTTCCTTTATCTTTCAAGTTTCTTTCTAAAGTGTTCTTGGTTTCTCTAATTGGGATTACTTTGAGCTTAAATCTTTATTACGTAGCAATCAGCCACACATCAGCAACATTTGCAGCTGCCACTACCAACACCATCCCTGCCATTACCCTCCTTTTGGCTCTCCTTTTCAGAAAGGTTGAAGGGATGGCCAAGTTGGTGGGAGGAGTGATAGGTTTTTCAGGGGCTTTAGTGTTTGCTTTTGTGAAGGGTCCACCAATGAAATTCATGAATTGGCATCCACAAACAAATAATAATAATAATTCAAATTCATTTCAAGCTTATTCCACATTAGAATGGATTAAGGGTTCATTCACTATGCTTTCAGCCAACATTGCTTGGTCTTTATGGCTTTGTTTCTTTAGTTTGATACAATCAGCTTTGTGGGCTTTGGTAATGGAGAGAAAGCCAAAAGCTTGGAAACTTGGATGGAATCTTCAACTCTTCTCTGTTGCCTATTGTTCTTATAATATGGGTATAATCGTGACCGGAATGACGTATTGGCTACAAATATGGAGTGTGGAGAAGAAAGGGCCAGTTTTCACAGCTATGTTTACACCATTAGCTCTCATCATAACAGCAATCTTCTCAGCATTATTATGGAAGGAAACCCTTCATTGGGGAAGGTCAATTCCTTTATTTTATTATTATTATTACTTTTAA

Protein sequence

MKSFVGYVEAMEVHKPYIAMLFVQCVYSGMALLSKAAISQGMNPSVFVFYRQAFATVAMAPLAFLLERKKAVPLSFKFLSKVFLVSLIGITLSLNLYYVAISHTSATFAAATTNTIPAITLLLALLFRKVEGMAKLVGGVIGFSGALVFAFVKGPPMKFMNWHPQTNNNNNSNSFQAYSTLEWIKGSFTMLSANIAWSLWLCFFSLIQSALWALVMERKPKAWKLGWNLQLFSVAYCSYNMGIIVTGMTYWLQIWSVEKKGPVFTAMFTPLALIITAIFSALLWKETLHWGRSIPLFYYYYYF

Homology

[Close]

BLAST of Lsi08G005540 vs. ExPASy Swiss-Prot
Match: Q6NMB7 (WAT1-related protein At1g43650 OS=Arabidopsis thaliana OX=3702 GN=At1g43650 PE=2 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 1.4e-77
Identity = 166/308 (53.90%), Postives = 210/308 (68.18%), Query Frame = 0


Query: 11  MEVHKPYIAMLFVQCVYSGMALLSKAAISQGMNPSVFVFYRQAFATVAMAPLAFLLERKK 70
           M  HK  +AM+FVQ VY+GM LLSK AISQG NP VFVFYRQAFA +A++P AF LE  K
Sbjct: 2   MMEHKANMAMVFVQIVYAGMPLLSKVAISQGTNPFVFVFYRQAFAALALSPFAFFLESSK 61

Query: 71  AVPLSFKFLSKVFLVSLIGITLSLNLYYVAISHTSATFAAATTNTIPAITLLLALLFR-- 130
           + PLSF  L K+F +SL G+TLSLNLYYVAI +T+ATFAAATTN IP+IT +LALLFR  
Sbjct: 62  SSPLSFILLLKIFFISLCGLTLSLNLYYVAIENTTATFAAATTNAIPSITFVLALLFRLE 121

Query: 131 -----KVEGMAKLVGGVIGFSGALVFAFVKGPPMKFMNWHPQTNNNNNSNSFQAYSTLEW 190
                K  G+AK+ G ++G  GALVFAFVKGP +  +N +    N++   +    ST   
Sbjct: 122 TVTLKKSHGVAKVTGSMVGMLGALVFAFVKGPSL--INHY----NSSTIPNGTVPSTKNS 181

Query: 191 IKGSFTMLSANIAWSLWL--------------------CFFSLIQSALWALVMERKPKAW 250
           +KGS TML+AN  W LW+                    C FS IQSA+WA+ + R P  W
Sbjct: 182 VKGSITMLAANTCWCLWIIMQSKVMKEYPAKLRLVALQCLFSCIQSAVWAVAVNRNPSVW 241

Query: 251 KLGWNLQLFSVAYCSYNMGIIVTGMTYWLQIWSVEKKGPVFTAMFTPLALIITAIFSALL 292
           K+ + L L S+AYC    GI+VTG+TYWLQ+W++EKKGPVFTA++TPLALI+T I S+ L
Sbjct: 242 KIEFGLPLLSMAYC----GIMVTGLTYWLQVWAIEKKGPVFTALYTPLALILTCIVSSFL 299

[Close]

BLAST of Lsi08G005540 vs. ExPASy Swiss-Prot
Match: Q9FGG3 (WAT1-related protein At5g64700 OS=Arabidopsis thaliana OX=3702 GN=At5g64700 PE=2 SV=1)

HSP 1 Score: 240.7 bits (613), Expect = 2.1e-62
Identity = 141/312 (45.19%), Postives = 192/312 (61.54%), Query Frame = 0


Query: 11  MEVHKPYIAMLFVQCVYSGMALLSKAAISQGMNPSVFVFYRQAFATVAMAPLAFLLERKK 70
           ME  KPY+ +  +Q +Y+ M L+SKA  + GMN  VFVFYRQAFAT+ +APLAF  ERK 
Sbjct: 3   MESKKPYLMVTIIQVIYTIMFLISKAVFNGGMNTFVFVFYRQAFATIFLAPLAFFFERKS 62

Query: 71  AVPLSFKFLSKVFLVSLIGITLSLNLYYVAISHTSATFAAATTNTIPAITLLLALLF--- 130
           A PLSF    K+F++SL G+TLSL+L  +A+S+TSAT AAATT ++PAIT  LALLF   
Sbjct: 63  APPLSFVTFIKIFMLSLFGVTLSLDLNGIALSYTSATLAAATTASLPAITFFLALLFGME 122

Query: 131 ----RKVEGMAKLVGGVIGFSGALVFAFVKGPPMKF----MNWHPQTNNNNNSNSFQAYS 190
               + ++G AKLVG  +   G ++ A  KGP +K       +H Q + + N+    +  
Sbjct: 123 RLKVKSIQGTAKLVGITVCMGGVIILAIYKGPLLKLPLCPHFYHGQEHPHRNNPGHVSGG 182

Query: 191 TLEWIKGSFTMLSANIAWSLWL--------------------CFFSLIQSALWALVMERK 250
           +  W+KG   M+++NI W LWL                    C  S IQS + A+ +ER 
Sbjct: 183 STSWLKGCVLMITSNILWGLWLVLQGRVLKVYPSKLYFTTLHCLLSSIQSFVIAIALERD 242

Query: 251 PKAWKLGWNLQLFSVAYCSYNMGIIVTGMTYWLQIWSVEKKGPVFTAMFTPLALIITAIF 292
             AWKLGWNL+L +V YC    G IVTG+ Y+LQ W +EK+GPVF +MFTPL+L+ T + 
Sbjct: 243 ISAWKLGWNLRLVAVIYC----GFIVTGVAYYLQSWVIEKRGPVFLSMFTPLSLLFTLLS 302

[Close]

BLAST of Lsi08G005540 vs. ExPASy Swiss-Prot
Match: Q9FL41 (WAT1-related protein At5g07050 OS=Arabidopsis thaliana OX=3702 GN=At5g07050 PE=2 SV=1)

HSP 1 Score: 166.8 bits (421), Expect = 3.9e-40
Identity = 100/310 (32.26%), Postives = 172/310 (55.48%), Query Frame = 0


Query: 15  KPYIAMLFVQCVYSGMALLSKAAISQGMNPSVFVFYRQAFATVAMAPLAFLLERKKAVPL 74
           KPY AM+ +Q  Y+GM +++K +++ GM+  V V YR A AT  +AP AF  ERK    +
Sbjct: 17  KPYFAMISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIAPFAFFFERKAQPKI 76

Query: 75  SFKFLSKVFLVSLIGITLSLNLYYVAISHTSATFAAATTNTIPAITLLLALLFR------ 134
           +F    ++F++ L+G  +  N YY+ + +TS TF+ A +N +PA+T +LA+LFR      
Sbjct: 77  TFSIFMQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMTFILAVLFRMEMLDL 136

Query: 135 -KVEGMAKLVGGVIGFSGALVFAFVKGPPMKFMNW----HPQTNNN-NNSNSFQAYSTLE 194
            K+   AK+ G V+  +GA++    KGP ++   W    H Q +++ N ++S  + S  E
Sbjct: 137 KKLWCQAKIAGTVVTVAGAMLMTIYKGPIVELF-WTKYMHIQDSSHANTTSSKNSSSDKE 196

Query: 195 WIKGSFTMLSANIAW---------------------SLWLCFFSLIQSALWALVMERKPK 254
           ++KGS  ++ A +AW                     +  +CF   +Q+     VME  P 
Sbjct: 197 FLKGSILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQAVAVTFVMEHNPS 256

Query: 255 AWKLGWNLQLFSVAYCSYNMGIIVTGMTYWLQIWSVEKKGPVFTAMFTPLALIITAIFSA 292
           AW++GW++ L + AY     GI+ + ++Y++Q   ++K+GPVF   F+PL ++I A+  +
Sbjct: 257 AWRIGWDMNLLAAAY----SGIVASSISYYVQGIVMKKRGPVFATAFSPLMMVIVAVMGS 316

[Close]

BLAST of Lsi08G005540 vs. ExPASy Swiss-Prot
Match: F4IJ08 (WAT1-related protein At2g40900 OS=Arabidopsis thaliana OX=3702 GN=At2g40900 PE=2 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 2.5e-39
Identity = 106/301 (35.22%), Postives = 170/301 (56.48%), Query Frame = 0


Query: 12  EVHKPYIAMLFVQCVYSGMALLSKAAISQGMNPSVFVFYRQAFATVAMAPLAFLLERKKA 71
           E  KPY AM+ +Q  Y+GM L++K  + +GM+  V V YR AFAT A+AP A L ERK  
Sbjct: 7   ESAKPYFAMVCLQFGYAGMNLVTKTVLDRGMSHYVLVAYRNAFATAAIAPFALLSERKVR 66

Query: 72  VPLSFKFLSKVFLVSLIGITLSLNLYYVAISHTSATFAAATTNTIPAITLLLALLF---- 131
             ++F    ++FL++L+G  +  NLYY+ +  TS TF++A +N +PAIT++LA LF    
Sbjct: 67  SKMTFPIFMRIFLLALLGPVIDQNLYYIGLKLTSPTFSSAVSNIVPAITIILATLFRMEK 126

Query: 132 ---RKVEGMAKLVGGVIGFSGALVFAFVKGPPMKFMNWH------PQTNNNNNSNSFQAY 191
              RKV  + K++G ++   G+++  F KGP + F   H      P T +   +  F   
Sbjct: 127 VEMRKVRCLVKVMGTLVTVVGSILMIFYKGPFINFFRSHLTAASSPPTADYLKAAVFLLL 186

Query: 192 STLEWIKGSFTML--------SANIAWSLWLCFFSLIQSALWALVMERKPKAWKLGWNLQ 251
           ++L W   SF +L        SA+++ S  +CF   +QS   A VME  P A  +G+++ 
Sbjct: 187 ASLSW--ASFFVLQAATLKKYSAHLSMSTMVCFMGTLQSLALAFVMEHNPSALNIGFDMN 246

Query: 252 LFSVAYCSYNMGIIVTGMTYWLQIWSVEKKGPVFTAMFTPLALIITAIFSALLWKETLHW 292
           L + AY     GI+ + + Y++Q   +++KGPVF   F PL ++I +I S  +  + ++ 
Sbjct: 247 LLASAYA----GIMSSSIAYYVQGLMMQRKGPVFVTAFNPLIVVIVSIMSFFVLGQGIYL 301

[Close]

BLAST of Lsi08G005540 vs. ExPASy Swiss-Prot
Match: F4HZQ7 (WAT1-related protein At1g21890 OS=Arabidopsis thaliana OX=3702 GN=At1g21890 PE=2 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 2.8e-38
Identity = 104/313 (33.23%), Postives = 162/313 (51.76%), Query Frame = 0


Query: 15  KPYIAMLFVQCVYSGMALLSKAAISQGMNPSVFVFYRQAFATVAMAPLAFLLERKKAVPL 74
           KPY+AM+ +Q  Y+GM +++  ++  GMN  V   YR A AT  +AP A   ERK    +
Sbjct: 10  KPYLAMISMQFGYAGMYIITMVSLKHGMNHYVLAVYRHAIATAVIAPFALFHERKIRPKM 69

Query: 75  SFKFLSKVFLVSLIGITLSLNLYYVAISHTSATFAAATTNTIPAITLLLALLFR------ 134
           +F+   ++ L+  I   L  NLYYV +++TSATFA+AT N +PAIT +LA++FR      
Sbjct: 70  TFRIFLQIALLGFIEPVLDQNLYYVGMTYTSATFASATANVLPAITFVLAIIFRLESVNF 129

Query: 135 -KVEGMAKLVGGVIGFSGALVFAFVKGPPMKFMNW------HPQTNNNNNSNSFQAYSTL 194
            KV  +AK+VG VI  SGAL+    KGP + F+ +             ++  +  A    
Sbjct: 130 KKVRSIAKVVGTVITVSGALLMTLYKGPIVDFIRFGGGGGGGSDGAGGSHGGAGAAAMDK 189

Query: 195 EWIKGSFTMLSANIAW--------------------SLWLCFFSLIQSALWALVMERKPK 254
            WI G+  +L     W                    +  +C    ++    +LV  R   
Sbjct: 190 HWIPGTLMLLGRTFGWAGFFILQSFTLKQYPAELSLTTLICLMGTLEGTAVSLVTVRDLS 249

Query: 255 AWKLGWNLQLFSVAYCSYNMGIIVTGMTYWLQIWSVEKKGPVFTAMFTPLALIITAIFSA 295
           AWK+G++  LF+ AY     G+I +G+ Y++Q   + ++GPVF A F PL ++ITA    
Sbjct: 250 AWKIGFDSNLFAAAY----SGVICSGVAYYVQGVVMRERGPVFVATFNPLCVVITAALGV 309

[Close]

BLAST of Lsi08G005540 vs. ExPASy TrEMBL
Match: A0A5D3DGQ0 (WAT1-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1163G00750 PE=3 SV=1)

HSP 1 Score: 486.1 bits (1250), Expect = 1.1e-133
Identity = 262/319 (82.13%), Postives = 275/319 (86.21%), Query Frame = 0


Query: 1   MKSFVGYVEAMEVHKPYIAMLFVQCVYSGMALLSKAAISQ-GMNPSVFVFYRQAFATVAM 60
           MKSF+GYVEAM VHKPYIAMLFVQCVYSGMAL SKAAISQ GMNP++FVFYRQAFATVAM
Sbjct: 1   MKSFLGYVEAMRVHKPYIAMLFVQCVYSGMALFSKAAISQKGMNPAIFVFYRQAFATVAM 60

Query: 61  APLAFLLERKKAVPLSFKFLSKVFLVSLIGITLSLNLYYVAISHTSATFAAATTNTIPAI 120
           APLAFLLERKK VPLSFKF SKVFLVSLIG+TLSLNLYYVAI+HTSATFAAATTNTIPAI
Sbjct: 61  APLAFLLERKKEVPLSFKFHSKVFLVSLIGVTLSLNLYYVAINHTSATFAAATTNTIPAI 120

Query: 121 TLLLALLF-------RKVEGMAKLVGGVIGFSGALVFAFVKGPPMKFMNWHPQTNNNNNS 180
           TLLLALLF       RKVEGMAKL+G +IGFSGALVFAFVKGPPMKFMNW+PQT  NN +
Sbjct: 121 TLLLALLFRYESICIRKVEGMAKLMGAIIGFSGALVFAFVKGPPMKFMNWYPQT--NNIT 180

Query: 181 NSFQAYSTLEWIKGSFTMLSANIAWSLWL--------------------CFFSLIQSALW 240
           NSFQ YSTLEWIKGSFTMLSAN+AWS WL                    CFFSLIQSALW
Sbjct: 181 NSFQPYSTLEWIKGSFTMLSANLAWSFWLVLQASIVKEYPAKLRVTTLQCFFSLIQSALW 240

Query: 241 ALVMERKPKAWKLGWNLQLFSVAYCSYNMGIIVTGMTYWLQIWSVEKKGPVFTAMFTPLA 292
           ALVMER P+AWKLGWNLQLFSVAYC    G+IVTGMTYWLQIW VEKKGPVFTAMFTPLA
Sbjct: 241 ALVMERNPQAWKLGWNLQLFSVAYC----GVIVTGMTYWLQIWCVEKKGPVFTAMFTPLA 300

[Close]

BLAST of Lsi08G005540 vs. ExPASy TrEMBL
Match: A0A1S3B3L9 (WAT1-related protein OS=Cucumis melo OX=3656 GN=LOC103485619 PE=3 SV=1)

HSP 1 Score: 486.1 bits (1250), Expect = 1.1e-133
Identity = 262/319 (82.13%), Postives = 275/319 (86.21%), Query Frame = 0


Query: 1   MKSFVGYVEAMEVHKPYIAMLFVQCVYSGMALLSKAAISQ-GMNPSVFVFYRQAFATVAM 60
           MKSF+GYVEAM VHKPYIAMLFVQCVYSGMAL SKAAISQ GMNP++FVFYRQAFATVAM
Sbjct: 1   MKSFLGYVEAMRVHKPYIAMLFVQCVYSGMALFSKAAISQKGMNPAIFVFYRQAFATVAM 60

Query: 61  APLAFLLERKKAVPLSFKFLSKVFLVSLIGITLSLNLYYVAISHTSATFAAATTNTIPAI 120
           APLAFLLERKK VPLSFKF SKVFLVSLIG+TLSLNLYYVAI+HTSATFAAATTNTIPAI
Sbjct: 61  APLAFLLERKKEVPLSFKFHSKVFLVSLIGVTLSLNLYYVAINHTSATFAAATTNTIPAI 120

Query: 121 TLLLALLF-------RKVEGMAKLVGGVIGFSGALVFAFVKGPPMKFMNWHPQTNNNNNS 180
           TLLLALLF       RKVEGMAKL+G +IGFSGALVFAFVKGPPMKFMNW+PQT  NN +
Sbjct: 121 TLLLALLFRYESICIRKVEGMAKLMGAIIGFSGALVFAFVKGPPMKFMNWYPQT--NNIT 180

Query: 181 NSFQAYSTLEWIKGSFTMLSANIAWSLWL--------------------CFFSLIQSALW 240
           NSFQ YSTLEWIKGSFTMLSAN+AWS WL                    CFFSLIQSALW
Sbjct: 181 NSFQPYSTLEWIKGSFTMLSANLAWSFWLVLQASIVKEYPAKLRVTTLQCFFSLIQSALW 240

Query: 241 ALVMERKPKAWKLGWNLQLFSVAYCSYNMGIIVTGMTYWLQIWSVEKKGPVFTAMFTPLA 292
           ALVMER P+AWKLGWNLQLFSVAYC    G+IVTGMTYWLQIW VEKKGPVFTAMFTPLA
Sbjct: 241 ALVMERNPQAWKLGWNLQLFSVAYC----GVIVTGMTYWLQIWCVEKKGPVFTAMFTPLA 300

[Close]

BLAST of Lsi08G005540 vs. ExPASy TrEMBL
Match: A0A6J1BVI1 (WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111006092 PE=3 SV=1)

HSP 1 Score: 465.7 bits (1197), Expect = 1.5e-127
Identity = 248/318 (77.99%), Postives = 265/318 (83.33%), Query Frame = 0


Query: 1   MKSFVGYVEAMEVHKPYIAMLFVQCVYSGMALLSKAAISQGMNPSVFVFYRQAFATVAMA 60
           MKSFVG VEAMEVHKPY+AMLFVQCVYSGMAL SKAAIS GMNP VFVFYRQAFAT+AMA
Sbjct: 1   MKSFVGCVEAMEVHKPYVAMLFVQCVYSGMALFSKAAISAGMNPPVFVFYRQAFATLAMA 60

Query: 61  PLAFLLERKKAVPLSFKFLSKVFLVSLIGITLSLNLYYVAISHTSATFAAATTNTIPAIT 120
           PLAF LERKKAVPLSFKFLSKVFLVSL GITLSLNLYY+AI+HTSATFAAATTNTIPAIT
Sbjct: 61  PLAFSLERKKAVPLSFKFLSKVFLVSLTGITLSLNLYYIAINHTSATFAAATTNTIPAIT 120

Query: 121 LLLALLF-------RKVEGMAKLVGGVIGFSGALVFAFVKGPPMKFMNWHPQTNNNNNSN 180
           LLLALLF       RK++G+AKL+G VIG SGALVFAFVKGPPMKFMNW+P+TNNN+   
Sbjct: 121 LLLALLFRYENIPIRKMQGIAKLMGAVIGLSGALVFAFVKGPPMKFMNWYPKTNNNDQIT 180

Query: 181 SFQAYSTLEWIKGSFTMLSANIAWSLWL--------------------CFFSLIQSALWA 240
              +YSTLEWIKGS  M+SANIAWSLWL                    CFFSLIQSALWA
Sbjct: 181 PSASYSTLEWIKGSLMMISANIAWSLWLVFQGSIVKEYPAKLRVTTLQCFFSLIQSALWA 240

Query: 241 LVMERKPKAWKLGWNLQLFSVAYCSYNMGIIVTGMTYWLQIWSVEKKGPVFTAMFTPLAL 292
           + MER P+AWKLGWNLQL SVAYC    G+IVTGMTYWLQIW+VEKKGPVF AMFTPLAL
Sbjct: 241 VAMERNPQAWKLGWNLQLISVAYC----GVIVTGMTYWLQIWTVEKKGPVFIAMFTPLAL 300

[Close]

BLAST of Lsi08G005540 vs. ExPASy TrEMBL
Match: A0A0A0KEE4 (WAT1-related protein OS=Cucumis sativus OX=3659 GN=Csa_6G056580 PE=3 SV=1)

HSP 1 Score: 463.4 bits (1191), Expect = 7.4e-127
Identity = 250/309 (80.91%), Postives = 263/309 (85.11%), Query Frame = 0


Query: 11  MEVHKPYIAMLFVQCVYSGMALLSKAAISQ-GMNPSVFVFYRQAFATVAMAPLAFLLERK 70
           M VHKPYIAMLFVQCVYSGMAL SKAAISQ GMNP++FVFYRQAFATVAMAPLAFL ERK
Sbjct: 1   MRVHKPYIAMLFVQCVYSGMALFSKAAISQKGMNPAIFVFYRQAFATVAMAPLAFLFERK 60

Query: 71  KAVPLSFKFLSKVFLVSLIGITLSLNLYYVAISHTSATFAAATTNTIPAITLLLALLF-- 130
           K VPLSFKF SKVF+VSLIG+TLSLNLYY+AI+HTSATFAAATTNTIPAITLLLALLF  
Sbjct: 61  KEVPLSFKFHSKVFVVSLIGVTLSLNLYYIAINHTSATFAAATTNTIPAITLLLALLFRY 120

Query: 131 -----RKVEGMAKLVGGVIGFSGALVFAFVKGPPMKFMNWHPQTNNNNNSNSFQAYSTLE 190
                RKVEGMAKLVG +IGFSGALVFAFVKGPPMKFMNW+PQT   N +NSFQ YSTLE
Sbjct: 121 ESICIRKVEGMAKLVGAIIGFSGALVFAFVKGPPMKFMNWYPQT--KNITNSFQPYSTLE 180

Query: 191 WIKGSFTMLSANIAWSLWL--------------------CFFSLIQSALWALVMERKPKA 250
           WIKG+FTMLSANIAWS WL                    CFFSLIQSALWALVMER P+A
Sbjct: 181 WIKGAFTMLSANIAWSFWLVLQGSIVKEYPAKLRITTLQCFFSLIQSALWALVMERNPQA 240

Query: 251 WKLGWNLQLFSVAYCSYNMGIIVTGMTYWLQIWSVEKKGPVFTAMFTPLALIITAIFSAL 292
           WKLGWNLQLFSVAYC    G+IVTGMTYWLQIW VEKKGPVFTAMFTPLALIITAIFSAL
Sbjct: 241 WKLGWNLQLFSVAYC----GVIVTGMTYWLQIWCVEKKGPVFTAMFTPLALIITAIFSAL 300

[Close]

BLAST of Lsi08G005540 vs. ExPASy TrEMBL
Match: A0A6J1HH16 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111463975 PE=3 SV=1)

HSP 1 Score: 455.3 bits (1170), Expect = 2.0e-124
Identity = 244/318 (76.73%), Postives = 259/318 (81.45%), Query Frame = 0


Query: 1   MKSFVGYVEAMEVHKPYIAMLFVQCVYSGMALLSKAAISQGMNPSVFVFYRQAFATVAMA 60
           M+S V Y EAMEVHKPYIAMLFVQCVYSGMAL SKAAISQGMNP +FVFYRQAFAT+AMA
Sbjct: 1   MRSLVSYAEAMEVHKPYIAMLFVQCVYSGMALFSKAAISQGMNPPIFVFYRQAFATIAMA 60

Query: 61  PLAFLLERKKAVPLSFKFLSKVFLVSLIGITLSLNLYYVAISHTSATFAAATTNTIPAIT 120
           P AF  ERKKAVPLSFKFL KVFL+SL GITLSLNLYY+AI+H SATFAAATTNTIPAIT
Sbjct: 61  PFAFFFERKKAVPLSFKFLFKVFLISLSGITLSLNLYYIAINHISATFAAATTNTIPAIT 120

Query: 121 LLLALLF-------RKVEGMAKLVGGVIGFSGALVFAFVKGPPMKFMNWHPQTNNNNNSN 180
           LL ALLF       RK+EG+AKLVGGVIGFSGALV+AFVKGP MKFMNW+PQ    N  N
Sbjct: 121 LLFALLFRYEVISVRKMEGIAKLVGGVIGFSGALVYAFVKGPVMKFMNWYPQ----NARN 180

Query: 181 SFQAYSTLEWIKGSFTMLSANIAWSLWL--------------------CFFSLIQSALWA 240
           SF+ YS  EWIKGSF MLSANIAWSLWL                    CFFSLIQS LWA
Sbjct: 181 SFEGYSGSEWIKGSFVMLSANIAWSLWLVLQASIVKEYPAKLRITSLQCFFSLIQSGLWA 240

Query: 241 LVMERKPKAWKLGWNLQLFSVAYCSYNMGIIVTGMTYWLQIWSVEKKGPVFTAMFTPLAL 292
           + MERK  AWKLGWNLQLFSVAYC    G+IVTGMTYWLQIW+VEKKGPVFTAMFTPLAL
Sbjct: 241 VAMERKADAWKLGWNLQLFSVAYC----GVIVTGMTYWLQIWTVEKKGPVFTAMFTPLAL 300

[Close]

BLAST of Lsi08G005540 vs. NCBI nr
Match: XP_038884292.1 (WAT1-related protein At1g43650 isoform X2 [Benincasa hispida])

HSP 1 Score: 503.8 bits (1296), Expect = 1.0e-138
Identity = 269/323 (83.28%), Postives = 280/323 (86.69%), Query Frame = 0


Query: 1   MKSFVGYVEAMEVHKPYIAMLFVQCVYSGMALLSKAAISQGMNPSVFVFYRQAFATVAMA 60
           MKSFVGYVEAMEVHKPYIAMLFVQCVYSGMAL SKAAISQGMNP +FVFYRQAFATVAMA
Sbjct: 1   MKSFVGYVEAMEVHKPYIAMLFVQCVYSGMALFSKAAISQGMNPPIFVFYRQAFATVAMA 60

Query: 61  PLAFLLERKKAVPLSFKFLSKVFLVSLIGITLSLNLYYVAISHTSATFAAATTNTIPAIT 120
           PLAFL ERKKAVPL FKFLSKVFLVSL+GITLSLNLYY+AI+HTSATFAAATTNTIPAIT
Sbjct: 61  PLAFLFERKKAVPLCFKFLSKVFLVSLVGITLSLNLYYIAINHTSATFAAATTNTIPAIT 120

Query: 121 LLLALLF-------RKVEGMAKLVGGVIGFSGALVFAFVKGPPMKFMNWHPQTNNNN--- 180
           LLLALLF       RKVEGMAKL+G +IGFSGALVFAFVKGPPMKFMNW+PQTNNNN   
Sbjct: 121 LLLALLFRYESICIRKVEGMAKLMGAMIGFSGALVFAFVKGPPMKFMNWYPQTNNNNNNN 180

Query: 181 --NSNSFQAYSTLEWIKGSFTMLSANIAWSLWL--------------------CFFSLIQ 240
             NSNSFQ YSTLEWIKGSFTMLSANIAWSLWL                    CFFSLIQ
Sbjct: 181 NSNSNSFQPYSTLEWIKGSFTMLSANIAWSLWLVLQGFIVKEYPAKLRITTLQCFFSLIQ 240

Query: 241 SALWALVMERKPKAWKLGWNLQLFSVAYCSYNMGIIVTGMTYWLQIWSVEKKGPVFTAMF 292
           SALWA+VMERKP+AWKLGWNLQLFSVAYC    G+IVTGMTYWLQIW VEKKGPVFTAMF
Sbjct: 241 SALWAVVMERKPQAWKLGWNLQLFSVAYC----GVIVTGMTYWLQIWCVEKKGPVFTAMF 300

[Close]

BLAST of Lsi08G005540 vs. NCBI nr
Match: XP_038884291.1 (WAT1-related protein At1g43650 isoform X1 [Benincasa hispida])

HSP 1 Score: 491.9 bits (1265), Expect = 4.0e-135
Identity = 269/354 (75.99%), Postives = 280/354 (79.10%), Query Frame = 0


Query: 1   MKSFVGYVEAMEVHKPYIAMLFVQCVYSGMALLSKAAISQGMNPSVFVFYRQAFATVAMA 60
           MKSFVGYVEAMEVHKPYIAMLFVQCVYSGMAL SKAAISQGMNP +FVFYRQAFATVAMA
Sbjct: 1   MKSFVGYVEAMEVHKPYIAMLFVQCVYSGMALFSKAAISQGMNPPIFVFYRQAFATVAMA 60

Query: 61  PLAFLLERKKAVPLSFKFLSKVFLVSLIGITLSLNLYYVAISHTSATFAAATTNTIPAIT 120
           PLAFL ERKKAVPL FKFLSKVFLVSL+GITLSLNLYY+AI+HTSATFAAATTNTIPAIT
Sbjct: 61  PLAFLFERKKAVPLCFKFLSKVFLVSLVGITLSLNLYYIAINHTSATFAAATTNTIPAIT 120

Query: 121 LLLALLF-------RKVEGMAKLVGGVIGFSGALVFAFVKGPPMKFMNWHPQTNNNN--- 180
           LLLALLF       RKVEGMAKL+G +IGFSGALVFAFVKGPPMKFMNW+PQTNNNN   
Sbjct: 121 LLLALLFRYESICIRKVEGMAKLMGAMIGFSGALVFAFVKGPPMKFMNWYPQTNNNNNNN 180

Query: 181 --NSNSFQAYSTLEWIKGSFTMLSANIAWSLWL--------------------------- 240
             NSNSFQ YSTLEWIKGSFTMLSANIAWSLWL                           
Sbjct: 181 NSNSNSFQPYSTLEWIKGSFTMLSANIAWSLWLVLQVLFLFYPSLSIFFTGFFHFFFFEE 240

Query: 241 ------------------------CFFSLIQSALWALVMERKPKAWKLGWNLQLFSVAYC 292
                                   CFFSLIQSALWA+VMERKP+AWKLGWNLQLFSVAYC
Sbjct: 241 IKFCSLKGFIVKEYPAKLRITTLQCFFSLIQSALWAVVMERKPQAWKLGWNLQLFSVAYC 300

[Close]

BLAST of Lsi08G005540 vs. NCBI nr
Match: XP_008441518.1 (PREDICTED: WAT1-related protein At1g43650 [Cucumis melo] >TYK22745.1 WAT1-related protein [Cucumis melo var. makuwa])

HSP 1 Score: 486.1 bits (1250), Expect = 2.2e-133
Identity = 262/319 (82.13%), Postives = 275/319 (86.21%), Query Frame = 0


Query: 1   MKSFVGYVEAMEVHKPYIAMLFVQCVYSGMALLSKAAISQ-GMNPSVFVFYRQAFATVAM 60
           MKSF+GYVEAM VHKPYIAMLFVQCVYSGMAL SKAAISQ GMNP++FVFYRQAFATVAM
Sbjct: 1   MKSFLGYVEAMRVHKPYIAMLFVQCVYSGMALFSKAAISQKGMNPAIFVFYRQAFATVAM 60

Query: 61  APLAFLLERKKAVPLSFKFLSKVFLVSLIGITLSLNLYYVAISHTSATFAAATTNTIPAI 120
           APLAFLLERKK VPLSFKF SKVFLVSLIG+TLSLNLYYVAI+HTSATFAAATTNTIPAI
Sbjct: 61  APLAFLLERKKEVPLSFKFHSKVFLVSLIGVTLSLNLYYVAINHTSATFAAATTNTIPAI 120

Query: 121 TLLLALLF-------RKVEGMAKLVGGVIGFSGALVFAFVKGPPMKFMNWHPQTNNNNNS 180
           TLLLALLF       RKVEGMAKL+G +IGFSGALVFAFVKGPPMKFMNW+PQT  NN +
Sbjct: 121 TLLLALLFRYESICIRKVEGMAKLMGAIIGFSGALVFAFVKGPPMKFMNWYPQT--NNIT 180

Query: 181 NSFQAYSTLEWIKGSFTMLSANIAWSLWL--------------------CFFSLIQSALW 240
           NSFQ YSTLEWIKGSFTMLSAN+AWS WL                    CFFSLIQSALW
Sbjct: 181 NSFQPYSTLEWIKGSFTMLSANLAWSFWLVLQASIVKEYPAKLRVTTLQCFFSLIQSALW 240

Query: 241 ALVMERKPKAWKLGWNLQLFSVAYCSYNMGIIVTGMTYWLQIWSVEKKGPVFTAMFTPLA 292
           ALVMER P+AWKLGWNLQLFSVAYC    G+IVTGMTYWLQIW VEKKGPVFTAMFTPLA
Sbjct: 241 ALVMERNPQAWKLGWNLQLFSVAYC----GVIVTGMTYWLQIWCVEKKGPVFTAMFTPLA 300

[Close]

BLAST of Lsi08G005540 vs. NCBI nr
Match: XP_004141013.1 (WAT1-related protein At1g43650 [Cucumis sativus] >KAE8646669.1 hypothetical protein Csa_005174 [Cucumis sativus])

HSP 1 Score: 482.6 bits (1241), Expect = 2.4e-132
Identity = 260/319 (81.50%), Postives = 273/319 (85.58%), Query Frame = 0


Query: 1   MKSFVGYVEAMEVHKPYIAMLFVQCVYSGMALLSKAAISQ-GMNPSVFVFYRQAFATVAM 60
           MKSFVGYVEAM VHKPYIAMLFVQCVYSGMAL SKAAISQ GMNP++FVFYRQAFATVAM
Sbjct: 1   MKSFVGYVEAMRVHKPYIAMLFVQCVYSGMALFSKAAISQKGMNPAIFVFYRQAFATVAM 60

Query: 61  APLAFLLERKKAVPLSFKFLSKVFLVSLIGITLSLNLYYVAISHTSATFAAATTNTIPAI 120
           APLAFL ERKK VPLSFKF SKVF+VSLIG+TLSLNLYY+AI+HTSATFAAATTNTIPAI
Sbjct: 61  APLAFLFERKKEVPLSFKFHSKVFVVSLIGVTLSLNLYYIAINHTSATFAAATTNTIPAI 120

Query: 121 TLLLALLF-------RKVEGMAKLVGGVIGFSGALVFAFVKGPPMKFMNWHPQTNNNNNS 180
           TLLLALLF       RKVEGMAKLVG +IGFSGALVFAFVKGPPMKFMNW+PQT   N +
Sbjct: 121 TLLLALLFRYESICIRKVEGMAKLVGAIIGFSGALVFAFVKGPPMKFMNWYPQT--KNIT 180

Query: 181 NSFQAYSTLEWIKGSFTMLSANIAWSLWL--------------------CFFSLIQSALW 240
           NSFQ YSTLEWIKG+FTMLSANIAWS WL                    CFFSLIQSALW
Sbjct: 181 NSFQPYSTLEWIKGAFTMLSANIAWSFWLVLQGSIVKEYPAKLRITTLQCFFSLIQSALW 240

Query: 241 ALVMERKPKAWKLGWNLQLFSVAYCSYNMGIIVTGMTYWLQIWSVEKKGPVFTAMFTPLA 292
           ALVMER P+AWKLGWNLQLFSVAYC    G+IVTGMTYWLQIW VEKKGPVFTAMFTPLA
Sbjct: 241 ALVMERNPQAWKLGWNLQLFSVAYC----GVIVTGMTYWLQIWCVEKKGPVFTAMFTPLA 300

[Close]

BLAST of Lsi08G005540 vs. NCBI nr
Match: XP_022133530.1 (WAT1-related protein At1g43650 [Momordica charantia])

HSP 1 Score: 465.7 bits (1197), Expect = 3.1e-127
Identity = 248/318 (77.99%), Postives = 265/318 (83.33%), Query Frame = 0


Query: 1   MKSFVGYVEAMEVHKPYIAMLFVQCVYSGMALLSKAAISQGMNPSVFVFYRQAFATVAMA 60
           MKSFVG VEAMEVHKPY+AMLFVQCVYSGMAL SKAAIS GMNP VFVFYRQAFAT+AMA
Sbjct: 1   MKSFVGCVEAMEVHKPYVAMLFVQCVYSGMALFSKAAISAGMNPPVFVFYRQAFATLAMA 60

Query: 61  PLAFLLERKKAVPLSFKFLSKVFLVSLIGITLSLNLYYVAISHTSATFAAATTNTIPAIT 120
           PLAF LERKKAVPLSFKFLSKVFLVSL GITLSLNLYY+AI+HTSATFAAATTNTIPAIT
Sbjct: 61  PLAFSLERKKAVPLSFKFLSKVFLVSLTGITLSLNLYYIAINHTSATFAAATTNTIPAIT 120

Query: 121 LLLALLF-------RKVEGMAKLVGGVIGFSGALVFAFVKGPPMKFMNWHPQTNNNNNSN 180
           LLLALLF       RK++G+AKL+G VIG SGALVFAFVKGPPMKFMNW+P+TNNN+   
Sbjct: 121 LLLALLFRYENIPIRKMQGIAKLMGAVIGLSGALVFAFVKGPPMKFMNWYPKTNNNDQIT 180

Query: 181 SFQAYSTLEWIKGSFTMLSANIAWSLWL--------------------CFFSLIQSALWA 240
              +YSTLEWIKGS  M+SANIAWSLWL                    CFFSLIQSALWA
Sbjct: 181 PSASYSTLEWIKGSLMMISANIAWSLWLVFQGSIVKEYPAKLRVTTLQCFFSLIQSALWA 240

Query: 241 LVMERKPKAWKLGWNLQLFSVAYCSYNMGIIVTGMTYWLQIWSVEKKGPVFTAMFTPLAL 292
           + MER P+AWKLGWNLQL SVAYC    G+IVTGMTYWLQIW+VEKKGPVF AMFTPLAL
Sbjct: 241 VAMERNPQAWKLGWNLQLISVAYC----GVIVTGMTYWLQIWTVEKKGPVFIAMFTPLAL 300

[Close]

BLAST of Lsi08G005540 vs. TAIR 10
Match: AT1G43650.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 291.2 bits (744), Expect = 9.6e-79
Identity = 166/308 (53.90%), Postives = 210/308 (68.18%), Query Frame = 0


Query: 11  MEVHKPYIAMLFVQCVYSGMALLSKAAISQGMNPSVFVFYRQAFATVAMAPLAFLLERKK 70
           M  HK  +AM+FVQ VY+GM LLSK AISQG NP VFVFYRQAFA +A++P AF LE  K
Sbjct: 2   MMEHKANMAMVFVQIVYAGMPLLSKVAISQGTNPFVFVFYRQAFAALALSPFAFFLESSK 61

Query: 71  AVPLSFKFLSKVFLVSLIGITLSLNLYYVAISHTSATFAAATTNTIPAITLLLALLFR-- 130
           + PLSF  L K+F +SL G+TLSLNLYYVAI +T+ATFAAATTN IP+IT +LALLFR  
Sbjct: 62  SSPLSFILLLKIFFISLCGLTLSLNLYYVAIENTTATFAAATTNAIPSITFVLALLFRLE 121

Query: 131 -----KVEGMAKLVGGVIGFSGALVFAFVKGPPMKFMNWHPQTNNNNNSNSFQAYSTLEW 190
                K  G+AK+ G ++G  GALVFAFVKGP +  +N +    N++   +    ST   
Sbjct: 122 TVTLKKSHGVAKVTGSMVGMLGALVFAFVKGPSL--INHY----NSSTIPNGTVPSTKNS 181

Query: 191 IKGSFTMLSANIAWSLWL--------------------CFFSLIQSALWALVMERKPKAW 250
           +KGS TML+AN  W LW+                    C FS IQSA+WA+ + R P  W
Sbjct: 182 VKGSITMLAANTCWCLWIIMQSKVMKEYPAKLRLVALQCLFSCIQSAVWAVAVNRNPSVW 241

Query: 251 KLGWNLQLFSVAYCSYNMGIIVTGMTYWLQIWSVEKKGPVFTAMFTPLALIITAIFSALL 292
           K+ + L L S+AYC    GI+VTG+TYWLQ+W++EKKGPVFTA++TPLALI+T I S+ L
Sbjct: 242 KIEFGLPLLSMAYC----GIMVTGLTYWLQVWAIEKKGPVFTALYTPLALILTCIVSSFL 299

[Close]

BLAST of Lsi08G005540 vs. TAIR 10
Match: AT5G64700.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 240.7 bits (613), Expect = 1.5e-63
Identity = 141/312 (45.19%), Postives = 192/312 (61.54%), Query Frame = 0


Query: 11  MEVHKPYIAMLFVQCVYSGMALLSKAAISQGMNPSVFVFYRQAFATVAMAPLAFLLERKK 70
           ME  KPY+ +  +Q +Y+ M L+SKA  + GMN  VFVFYRQAFAT+ +APLAF  ERK 
Sbjct: 3   MESKKPYLMVTIIQVIYTIMFLISKAVFNGGMNTFVFVFYRQAFATIFLAPLAFFFERKS 62

Query: 71  AVPLSFKFLSKVFLVSLIGITLSLNLYYVAISHTSATFAAATTNTIPAITLLLALLF--- 130
           A PLSF    K+F++SL G+TLSL+L  +A+S+TSAT AAATT ++PAIT  LALLF   
Sbjct: 63  APPLSFVTFIKIFMLSLFGVTLSLDLNGIALSYTSATLAAATTASLPAITFFLALLFGME 122

Query: 131 ----RKVEGMAKLVGGVIGFSGALVFAFVKGPPMKF----MNWHPQTNNNNNSNSFQAYS 190
               + ++G AKLVG  +   G ++ A  KGP +K       +H Q + + N+    +  
Sbjct: 123 RLKVKSIQGTAKLVGITVCMGGVIILAIYKGPLLKLPLCPHFYHGQEHPHRNNPGHVSGG 182

Query: 191 TLEWIKGSFTMLSANIAWSLWL--------------------CFFSLIQSALWALVMERK 250
           +  W+KG   M+++NI W LWL                    C  S IQS + A+ +ER 
Sbjct: 183 STSWLKGCVLMITSNILWGLWLVLQGRVLKVYPSKLYFTTLHCLLSSIQSFVIAIALERD 242

Query: 251 PKAWKLGWNLQLFSVAYCSYNMGIIVTGMTYWLQIWSVEKKGPVFTAMFTPLALIITAIF 292
             AWKLGWNL+L +V YC    G IVTG+ Y+LQ W +EK+GPVF +MFTPL+L+ T + 
Sbjct: 243 ISAWKLGWNLRLVAVIYC----GFIVTGVAYYLQSWVIEKRGPVFLSMFTPLSLLFTLLS 302

[Close]

BLAST of Lsi08G005540 vs. TAIR 10
Match: AT1G43650.2 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 190.7 bits (483), Expect = 1.8e-48
Identity = 113/198 (57.07%), Postives = 140/198 (70.71%), Query Frame = 0


Query: 11  MEVHKPYIAMLFVQCVYSGMALLSKAAISQGMNPSVFVFYRQAFATVAMAPLAFLLERKK 70
           M  HK  +AM+FVQ VY+GM LLSK AISQG NP VFVFYRQAFA +A++P AF LE  K
Sbjct: 2   MMEHKANMAMVFVQIVYAGMPLLSKVAISQGTNPFVFVFYRQAFAALALSPFAFFLESSK 61

Query: 71  AVPLSFKFLSKVFLVSLIGITLSLNLYYVAISHTSATFAAATTNTIPAITLLLALLFR-- 130
           + PLSF  L K+F +SL G+TLSLNLYYVAI +T+ATFAAATTN IP+IT +LALLFR  
Sbjct: 62  SSPLSFILLLKIFFISLCGLTLSLNLYYVAIENTTATFAAATTNAIPSITFVLALLFRLE 121

Query: 131 -----KVEGMAKLVGGVIGFSGALVFAFVKGPPMKFMNWHPQTNNNNNSNSFQAYSTLEW 190
                K  G+AK+ G ++G  GALVFAFVKGP +  +N +    N++   +    ST   
Sbjct: 122 TVTLKKSHGVAKVTGSMVGMLGALVFAFVKGPSL--INHY----NSSTIPNGTVPSTKNS 181

Query: 191 IKGSFTMLSANIAWSLWL 202
           +KGS TML+AN  W LW+
Sbjct: 182 VKGSITMLAANTCWCLWI 193

[Close]

BLAST of Lsi08G005540 vs. TAIR 10
Match: AT5G07050.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 166.8 bits (421), Expect = 2.7e-41
Identity = 100/310 (32.26%), Postives = 172/310 (55.48%), Query Frame = 0


Query: 15  KPYIAMLFVQCVYSGMALLSKAAISQGMNPSVFVFYRQAFATVAMAPLAFLLERKKAVPL 74
           KPY AM+ +Q  Y+GM +++K +++ GM+  V V YR A AT  +AP AF  ERK    +
Sbjct: 17  KPYFAMISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIAPFAFFFERKAQPKI 76

Query: 75  SFKFLSKVFLVSLIGITLSLNLYYVAISHTSATFAAATTNTIPAITLLLALLFR------ 134
           +F    ++F++ L+G  +  N YY+ + +TS TF+ A +N +PA+T +LA+LFR      
Sbjct: 77  TFSIFMQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMTFILAVLFRMEMLDL 136

Query: 135 -KVEGMAKLVGGVIGFSGALVFAFVKGPPMKFMNW----HPQTNNN-NNSNSFQAYSTLE 194
            K+   AK+ G V+  +GA++    KGP ++   W    H Q +++ N ++S  + S  E
Sbjct: 137 KKLWCQAKIAGTVVTVAGAMLMTIYKGPIVELF-WTKYMHIQDSSHANTTSSKNSSSDKE 196

Query: 195 WIKGSFTMLSANIAW---------------------SLWLCFFSLIQSALWALVMERKPK 254
           ++KGS  ++ A +AW                     +  +CF   +Q+     VME  P 
Sbjct: 197 FLKGSILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQAVAVTFVMEHNPS 256

Query: 255 AWKLGWNLQLFSVAYCSYNMGIIVTGMTYWLQIWSVEKKGPVFTAMFTPLALIITAIFSA 292
           AW++GW++ L + AY     GI+ + ++Y++Q   ++K+GPVF   F+PL ++I A+  +
Sbjct: 257 AWRIGWDMNLLAAAY----SGIVASSISYYVQGIVMKKRGPVFATAFSPLMMVIVAVMGS 316

[Close]

BLAST of Lsi08G005540 vs. TAIR 10
Match: AT2G40900.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 164.1 bits (414), Expect = 1.8e-40
Identity = 106/301 (35.22%), Postives = 170/301 (56.48%), Query Frame = 0


Query: 12  EVHKPYIAMLFVQCVYSGMALLSKAAISQGMNPSVFVFYRQAFATVAMAPLAFLLERKKA 71
           E  KPY AM+ +Q  Y+GM L++K  + +GM+  V V YR AFAT A+AP A L ERK  
Sbjct: 7   ESAKPYFAMVCLQFGYAGMNLVTKTVLDRGMSHYVLVAYRNAFATAAIAPFALLSERKVR 66

Query: 72  VPLSFKFLSKVFLVSLIGITLSLNLYYVAISHTSATFAAATTNTIPAITLLLALLF---- 131
             ++F    ++FL++L+G  +  NLYY+ +  TS TF++A +N +PAIT++LA LF    
Sbjct: 67  SKMTFPIFMRIFLLALLGPVIDQNLYYIGLKLTSPTFSSAVSNIVPAITIILATLFRMEK 126

Query: 132 ---RKVEGMAKLVGGVIGFSGALVFAFVKGPPMKFMNWH------PQTNNNNNSNSFQAY 191
              RKV  + K++G ++   G+++  F KGP + F   H      P T +   +  F   
Sbjct: 127 VEMRKVRCLVKVMGTLVTVVGSILMIFYKGPFINFFRSHLTAASSPPTADYLKAAVFLLL 186

Query: 192 STLEWIKGSFTML--------SANIAWSLWLCFFSLIQSALWALVMERKPKAWKLGWNLQ 251
           ++L W   SF +L        SA+++ S  +CF   +QS   A VME  P A  +G+++ 
Sbjct: 187 ASLSW--ASFFVLQAATLKKYSAHLSMSTMVCFMGTLQSLALAFVMEHNPSALNIGFDMN 246

Query: 252 LFSVAYCSYNMGIIVTGMTYWLQIWSVEKKGPVFTAMFTPLALIITAIFSALLWKETLHW 292
           L + AY     GI+ + + Y++Q   +++KGPVF   F PL ++I +I S  +  + ++ 
Sbjct: 247 LLASAYA----GIMSSSIAYYVQGLMMQRKGPVFVTAFNPLIVVIVSIMSFFVLGQGIYL 301

The following BLAST results are available for this feature:

BLAST of Lsi08G005540 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6NMB7	1.4e-77	53.90	WAT1-related protein At1g43650 OS=Arabidopsis thaliana OX=3702 GN=At1g43650 PE=2...	[more]
Q9FGG3	2.1e-62	45.19	WAT1-related protein At5g64700 OS=Arabidopsis thaliana OX=3702 GN=At5g64700 PE=2...	[more]
Q9FL41	3.9e-40	32.26	WAT1-related protein At5g07050 OS=Arabidopsis thaliana OX=3702 GN=At5g07050 PE=2...	[more]
F4IJ08	2.5e-39	35.22	WAT1-related protein At2g40900 OS=Arabidopsis thaliana OX=3702 GN=At2g40900 PE=2...	[more]
F4HZQ7	2.8e-38	33.23	WAT1-related protein At1g21890 OS=Arabidopsis thaliana OX=3702 GN=At1g21890 PE=2...	[more]

BLAST of Lsi08G005540 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3DGQ0	1.1e-133	82.13	WAT1-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold116...	[more]
A0A1S3B3L9	1.1e-133	82.13	WAT1-related protein OS=Cucumis melo OX=3656 GN=LOC103485619 PE=3 SV=1	[more]
A0A6J1BVI1	1.5e-127	77.99	WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111006092 PE=3 SV=1	[more]
A0A0A0KEE4	7.4e-127	80.91	WAT1-related protein OS=Cucumis sativus OX=3659 GN=Csa_6G056580 PE=3 SV=1	[more]
A0A6J1HH16	2.0e-124	76.73	WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111463975 PE=3 SV=1	[more]

BLAST of Lsi08G005540 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038884292.1	1.0e-138	83.28	WAT1-related protein At1g43650 isoform X2 [Benincasa hispida]	[more]
XP_038884291.1	4.0e-135	75.99	WAT1-related protein At1g43650 isoform X1 [Benincasa hispida]	[more]
XP_008441518.1	2.2e-133	82.13	PREDICTED: WAT1-related protein At1g43650 [Cucumis melo] >TYK22745.1 WAT1-relate...	[more]
XP_004141013.1	2.4e-132	81.50	WAT1-related protein At1g43650 [Cucumis sativus] >KAE8646669.1 hypothetical prot...	[more]
XP_022133530.1	3.1e-127	77.99	WAT1-related protein At1g43650 [Momordica charantia]	[more]

BLAST of Lsi08G005540 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G43650.1	9.6e-79	53.90	nodulin MtN21 /EamA-like transporter family protein	[more]
AT5G64700.1	1.5e-63	45.19	nodulin MtN21 /EamA-like transporter family protein	[more]
AT1G43650.2	1.8e-48	57.07	nodulin MtN21 /EamA-like transporter family protein	[more]
AT5G07050.1	2.7e-41	32.26	nodulin MtN21 /EamA-like transporter family protein	[more]
AT2G40900.1	1.8e-40	35.22	nodulin MtN21 /EamA-like transporter family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000620	EamA domain	PFAM	PF00892	EamA	coord: 15..149 e-value: 6.2E-14 score: 52.3
IPR030184	WAT1-related protein	PANTHER	PTHR31218	WAT1-RELATED PROTEIN	coord: 202..292 coord: 13..201
None	No IPR available	PANTHER	PTHR31218:SF183	WAT1-RELATED PROTEIN	coord: 202..292 coord: 13..201
None	No IPR available	SUPERFAMILY	103481	Multidrug resistance efflux transporter EmrE	coord: 50..151

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi08G005540.1	Lsi08G005540.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
cellular_component	GO:0016020	membrane
molecular_function	GO:0022857	transmembrane transporter activity