Lsi08G005140 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi08G005140
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	thioredoxin-like
Location	chr08: 13066182 .. 13067163 (+)
RNA-Seq Expression	Lsi08G005140
Synteny	Lsi08G005140

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAAAAAAAAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAATCCCAGCATTTTCCCTTCATACATAATACAAAAACAAACAGCCCCCAGTTGCCCTAGTTTATCCTTCTCCAATGGCACTCGATAACTGTATACAACTGAGCACCGCCGCCGCCATGTGCGCCACCACTTCCAGACCCAAATCTCCCTTTTTCTCTTCAAATTACCCCCAAAAGACATCCTTCACTGCCGTCTCCCTTCACCGTCTTAACAAATTACCCACTTCCGGACTCAGATTCTCCTCCCCTTCTTCCTCCACTTTCAAAAAATCCTTCTCCATCACGTGTCAAGCTCGTGGAGCCGTTGACGACGGTGAGTCCTCCGATCCATTTCTATGAGTTCTTCGCTTCTTCTGGCTTCGTGGGGTTTGTAAATTTGATGGGATTTTTTCTTTGTTAAGTGCAGTGAAAGAAGTGACAGAGTCGAGTTGGAACAATTTGGTGGTTGAGAATCAAAAGGCTGTGCTGGTGGAATTTTGGGCGCCATGGTGTGGACCTTGTAAGATTATTGAGCCTGTGATTAAGGAATTAGCTCAGGAATATGCAGGGAAGATTGTTTGTTTGAAGCTTAATACGGATATTAGTCCAAATGTGGCCTCTAAATATGGGATTAGAAGCATTCCAACGGTTCTGTTTTTCAAGAATGGAGAGAAGAGAGAAAGTGTTATTGGGGCTGTGCCTAAATCTACTTTGGCTGCCACCATTGATAAGTATGTCGAGGTTTGATTTTGAAGAATTCCCTCATCATCCAATTTCACCACATACCTTCCAATCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTGTATATGTATATCGTCATGTGTAAAAATGGGAAAACTTTTGTTAATCTCTTCTTTAATTCTGTTGTAACGATGACTTTCTTTTTCTACTTTGTGTACTGAGATCATTTTATGTTTATTTATTTATTTGGTGGTGGG

mRNA sequence

AAAAAAAAAAAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAATCCCAGCATTTTCCCTTCATACATAATACAAAAACAAACAGCCCCCAGTTGCCCTAGTTTATCCTTCTCCAATGGCACTCGATAACTGTATACAACTGAGCACCGCCGCCGCCATGTGCGCCACCACTTCCAGACCCAAATCTCCCTTTTTCTCTTCAAATTACCCCCAAAAGACATCCTTCACTGCCGTCTCCCTTCACCGTCTTAACAAATTACCCACTTCCGGACTCAGATTCTCCTCCCCTTCTTCCTCCACTTTCAAAAAATCCTTCTCCATCACGTGTCAAGCTCGTGGAGCCGTTGACGACGTGAAAGAAGTGACAGAGTCGAGTTGGAACAATTTGGTGGTTGAGAATCAAAAGGCTGTGCTGGTGGAATTTTGGGCGCCATGGTGTGGACCTTGTAAGATTATTGAGCCTGTGATTAAGGAATTAGCTCAGGAATATGCAGGGAAGATTGTTTGTTTGAAGCTTAATACGGATATTAGTCCAAATGTGGCCTCTAAATATGGGATTAGAAGCATTCCAACGGTTCTGTTTTTCAAGAATGGAGAGAAGAGAGAAAGTGTTATTGGGGCTGTGCCTAAATCTACTTTGGCTGCCACCATTGATAAGTATGTCGAGGTTTGATTTTGAAGAATTCCCTCATCATCCAATTTCACCACATACCTTCCAATCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTGTATATGTATATCGTCATGTGTAAAAATGGGAAAACTTTTGTTAATCTCTTCTTTAATTCTGTTGTAACGATGACTTTCTTTTTCTACTTTGTGTACTGAGATCATTTTATGTTTATTTATTTATTTGGTGGTGGG

Coding sequence (CDS)

ATGGCACTCGATAACTGTATACAACTGAGCACCGCCGCCGCCATGTGCGCCACCACTTCCAGACCCAAATCTCCCTTTTTCTCTTCAAATTACCCCCAAAAGACATCCTTCACTGCCGTCTCCCTTCACCGTCTTAACAAATTACCCACTTCCGGACTCAGATTCTCCTCCCCTTCTTCCTCCACTTTCAAAAAATCCTTCTCCATCACGTGTCAAGCTCGTGGAGCCGTTGACGACGTGAAAGAAGTGACAGAGTCGAGTTGGAACAATTTGGTGGTTGAGAATCAAAAGGCTGTGCTGGTGGAATTTTGGGCGCCATGGTGTGGACCTTGTAAGATTATTGAGCCTGTGATTAAGGAATTAGCTCAGGAATATGCAGGGAAGATTGTTTGTTTGAAGCTTAATACGGATATTAGTCCAAATGTGGCCTCTAAATATGGGATTAGAAGCATTCCAACGGTTCTGTTTTTCAAGAATGGAGAGAAGAGAGAAAGTGTTATTGGGGCTGTGCCTAAATCTACTTTGGCTGCCACCATTGATAAGTATGTCGAGGTTTGA

Protein sequence

MALDNCIQLSTAAAMCATTSRPKSPFFSSNYPQKTSFTAVSLHRLNKLPTSGLRFSSPSSSTFKKSFSITCQARGAVDDVKEVTESSWNNLVVENQKAVLVEFWAPWCGPCKIIEPVIKELAQEYAGKIVCLKLNTDISPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPKSTLAATIDKYVEV

Homology

[Close]

BLAST of Lsi08G005140 vs. ExPASy Swiss-Prot
Match: P07591 (Thioredoxin M-type, chloroplastic OS=Spinacia oleracea OX=3562 PE=1 SV=2)

HSP 1 Score: 174.9 bits (442), Expect = 8.6e-43
Identity = 93/191 (48.69%), Postives = 127/191 (66.49%), Query Frame = 0


Query: 1   MALDNCIQLSTAAAMCATTSRPK----SPFFSSNYPQ----KTSFTAVSLHRLNKLPTSG 60
           MA++NC+QLST+A++     +       P    N P     K SF A+S         S 
Sbjct: 1   MAIENCLQLSTSASVGTVAVKSHVHHLQPSSKVNVPTFRGLKRSFPALS---------SS 60

Query: 61  LRFSSPSSSTFKKSFSITCQARGAVDDVKEVTESSWNNLVVENQKAVLVEFWAPWCGPCK 120
           +  SSP    +    S+ C+A  AV +V++V +SSW   V+E++  V+V+FWAPWCGPCK
Sbjct: 61  VSSSSPRQFRYS---SVVCKASEAVKEVQDVNDSSWKEFVLESEVPVMVDFWAPWCGPCK 120

Query: 121 IIEPVIKELAQEYAGKIVCLKLNTDISPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPK 180
           +I PVI ELA+EY+GKI   KLNTD +P +A++Y IRSIPTVLFFKNGE++ES+IGAVPK
Sbjct: 121 LIAPVIDELAKEYSGKIAVYKLNTDEAPGIATQYNIRSIPTVLFFKNGERKESIIGAVPK 179

Query: 181 STLAATIDKYV 184
           STL  +I+KY+
Sbjct: 181 STLTDSIEKYL 179

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BLAST of Lsi08G005140 vs. ExPASy Swiss-Prot
Match: Q9ZP20 (Thioredoxin M5, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=TRXM PE=2 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 3.6e-41
Identity = 79/130 (60.77%), Postives = 97/130 (74.62%), Query Frame = 0


Query: 54  RFSSPSSSTFKKSFSITCQARGAVDDVKEVTESSWNNLVVENQKAVLVEFWAPWCGPCKI 113
           R S  S S  + +    CQ    VD+V    E +W+++V+ ++  VLVEFWAPWCGPC++
Sbjct: 41  RLSVASPSPLRPASRFACQCSNVVDEVVVADEKNWDSMVLGSEAPVLVEFWAPWCGPCRM 100

Query: 114 IEPVIKELAQEYAGKIVCLKLNTDISPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPKS 173
           I PVI ELA+EY GKI C K+NTD SPN+A+ YGIRSIPTVL FKNGEK+ESVIGAVPK+
Sbjct: 101 IAPVIDELAKEYVGKIKCCKVNTDDSPNIATNYGIRSIPTVLMFKNGEKKESVIGAVPKT 160

Query: 174 TLAATIDKYV 184
           TLA  IDKYV
Sbjct: 161 TLATIIDKYV 170

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BLAST of Lsi08G005140 vs. ExPASy Swiss-Prot
Match: Q41864 (Thioredoxin M-type, chloroplastic OS=Zea mays OX=4577 GN=TRM1 PE=2 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 6.2e-41
Identity = 77/113 (68.14%), Postives = 91/113 (80.53%), Query Frame = 0


Query: 71  CQARGAVDDVKEVTESSWNNLVVENQKAVLVEFWAPWCGPCKIIEPVIKELAQEYAGKIV 130
           CQ++ AVD+V    E +W+ LV+  +  VLVEFWAPWCGPC++I PVI ELA++YAGKI 
Sbjct: 52  CQSKNAVDEVVVADEKNWDGLVMACETPVLVEFWAPWCGPCRMIAPVIDELAKDYAGKIT 111

Query: 131 CLKLNTDISPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPKSTLAATIDKYV 184
           C K+NTD SPNVAS YGIRSIPTVL FK GEK+ESVIGAVPKSTL   IDKY+
Sbjct: 112 CCKVNTDDSPNVASTYGIRSIPTVLIFKGGEKKESVIGAVPKSTLTTLIDKYI 164

[Close]

BLAST of Lsi08G005140 vs. ExPASy Swiss-Prot
Match: P48384 (Thioredoxin M-type, chloroplastic OS=Pisum sativum OX=3888 PE=2 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 6.2e-41
Identity = 74/115 (64.35%), Postives = 97/115 (84.35%), Query Frame = 0


Query: 69  ITCQARGAVDDVKEVTESSWNNLVVENQKAVLVEFWAPWCGPCKIIEPVIKELAQEYAGK 128
           +   AR AV++V+ V +SSW+ LV+ ++  VLV+FWAPWCGPC++I P+I ELA+EYAGK
Sbjct: 58  VLLHAREAVNEVQVVNDSSWDELVIGSETPVLVDFWAPWCGPCRMIAPIIDELAKEYAGK 117

Query: 129 IVCLKLNTDISPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPKSTLAATIDKYV 184
           I C KLNTD SPN A+KYGIRSIPTVLFFKNGE+++SVIGAVPK+TL+  ++KY+
Sbjct: 118 IKCYKLNTDESPNTATKYGIRSIPTVLFFKNGERKDSVIGAVPKATLSEKVEKYI 172

[Close]

BLAST of Lsi08G005140 vs. ExPASy Swiss-Prot
Match: Q9ZP21 (Thioredoxin M-type, chloroplastic OS=Triticum aestivum OX=4565 PE=2 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 8.4e-38
Identity = 84/187 (44.92%), Postives = 113/187 (60.43%), Query Frame = 0


Query: 1   MALDNCIQ-LSTAAAMCATTSRPKSPFFSSNYPQKTSFTAVSLHRLNKLPTSGLRFSSPS 60
           MAL+ C++  +  A       R  S  ++ + P+  +   VS               SP 
Sbjct: 1   MALETCLRGWALYAPQAGIRERLSSGSYAPSRPRTAAPAVVS--------------PSPY 60

Query: 61  SSTF---KKSFSITCQARGAVDDVKEVTESSWNNLVVENQKAVLVEFWAPWCGPCKIIEP 120
            S     ++     C+ +  VD+V    E +W+N+V+  +  VLVEFWAPWCGPC++I P
Sbjct: 61  KSALVAARRPSRFVCKCKNVVDEVIVADEKNWDNMVIACESPVLVEFWAPWCGPCRMIAP 120

Query: 121 VIKELAQEYAGKIVCLKLNTDISPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPKSTLA 180
           VI ELA++Y GKI C K+NTD  PN+AS YGIRSIPTVL FK+GEK+ESVIGAVPK+TL 
Sbjct: 121 VIDELAKDYVGKIKCCKVNTDDCPNIASTYGIRSIPTVLMFKDGEKKESVIGAVPKTTLC 173

Query: 181 ATIDKYV 184
             IDKY+
Sbjct: 181 TIIDKYI 173

[Close]

BLAST of Lsi08G005140 vs. ExPASy TrEMBL
Match: A0A0A0KIC1 (Thioredoxin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G343710 PE=4 SV=1)

HSP 1 Score: 303.5 bits (776), Expect = 5.9e-79
Identity = 155/187 (82.89%), Postives = 163/187 (87.17%), Query Frame = 0


Query: 1   MALDNCIQLSTAAAMCATTSRPKSPFFSS--NYPQKTSFTAVSLHRLNKLPTSGLRFSSP 60
           MALDNCIQLSTAA MCA  S PKS +FSS  NYPQKTSF+ +SL RLNK PT        
Sbjct: 1   MALDNCIQLSTAATMCAAASIPKSHYFSSTTNYPQKTSFSTLSLQRLNKSPT-------- 60

Query: 61  SSSTFKKSFSITCQARGAVDDVKEVTESSWNNLVVENQKAVLVEFWAPWCGPCKIIEPVI 120
              TF+K FS+TCQARGAVDDVKEVTESSWNNLVVENQKAVLVEFWAPWCGPCKIIEPVI
Sbjct: 61  --PTFRKPFSLTCQARGAVDDVKEVTESSWNNLVVENQKAVLVEFWAPWCGPCKIIEPVI 120

Query: 121 KELAQEYAGKIVCLKLNTDISPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPKSTLAAT 180
           KELA EYAGKIVCLKLNTD+SPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPKSTL A+
Sbjct: 121 KELAAEYAGKIVCLKLNTDLSPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPKSTLTAS 177

Query: 181 IDKYVEV 186
           IDKY+EV
Sbjct: 181 IDKYIEV 177

[Close]

BLAST of Lsi08G005140 vs. ExPASy TrEMBL
Match: A0A5D3DJJ9 (Thioredoxin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1607G00860 PE=4 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 5.0e-78
Identity = 153/187 (81.82%), Postives = 162/187 (86.63%), Query Frame = 0


Query: 1   MALDNCIQLSTAAAMCATTSRPKSPFFSS--NYPQKTSFTAVSLHRLNKLPTSGLRFSSP 60
           MALDNCIQLSTAA MCA  S PKS +FSS  N+PQK SF+ +SL RLNK PT        
Sbjct: 1   MALDNCIQLSTAATMCAAASIPKSHYFSSTANFPQKLSFSTLSLQRLNKFPT-------- 60

Query: 61  SSSTFKKSFSITCQARGAVDDVKEVTESSWNNLVVENQKAVLVEFWAPWCGPCKIIEPVI 120
              TF+K FS+TCQARGAVDDVKEVTESSWNNLVVENQKAVLVEFWAPWCGPCKIIEPVI
Sbjct: 61  --PTFRKPFSVTCQARGAVDDVKEVTESSWNNLVVENQKAVLVEFWAPWCGPCKIIEPVI 120

Query: 121 KELAQEYAGKIVCLKLNTDISPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPKSTLAAT 180
           KELA EYAGKI CLKLNTD+SPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPKSTLAA+
Sbjct: 121 KELAAEYAGKIECLKLNTDVSPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPKSTLAAS 177

Query: 181 IDKYVEV 186
           IDKY+EV
Sbjct: 181 IDKYIEV 177

[Close]

BLAST of Lsi08G005140 vs. ExPASy TrEMBL
Match: A0A1S4DTR8 (thioredoxin-like OS=Cucumis melo OX=3656 GN=LOC107990489 PE=4 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 5.0e-78
Identity = 153/187 (81.82%), Postives = 162/187 (86.63%), Query Frame = 0


Query: 1   MALDNCIQLSTAAAMCATTSRPKSPFFSS--NYPQKTSFTAVSLHRLNKLPTSGLRFSSP 60
           MALDNCIQLSTAA MCA  S PKS +FSS  N+PQK SF+ +SL RLNK PT        
Sbjct: 1   MALDNCIQLSTAATMCAAASIPKSHYFSSTANFPQKLSFSTLSLQRLNKFPT-------- 60

Query: 61  SSSTFKKSFSITCQARGAVDDVKEVTESSWNNLVVENQKAVLVEFWAPWCGPCKIIEPVI 120
              TF+K FS+TCQARGAVDDVKEVTESSWNNLVVENQKAVLVEFWAPWCGPCKIIEPVI
Sbjct: 61  --PTFRKPFSVTCQARGAVDDVKEVTESSWNNLVVENQKAVLVEFWAPWCGPCKIIEPVI 120

Query: 121 KELAQEYAGKIVCLKLNTDISPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPKSTLAAT 180
           KELA EYAGKI CLKLNTD+SPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPKSTLAA+
Sbjct: 121 KELAAEYAGKIECLKLNTDVSPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPKSTLAAS 177

Query: 181 IDKYVEV 186
           IDKY+EV
Sbjct: 181 IDKYIEV 177

[Close]

BLAST of Lsi08G005140 vs. ExPASy TrEMBL
Match: A0A6J1JL70 (uncharacterized protein LOC111486882 OS=Cucurbita maxima OX=3661 GN=LOC111486882 PE=4 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 1.4e-72
Identity = 148/189 (78.31%), Postives = 162/189 (85.71%), Query Frame = 0


Query: 1   MALDNCIQLSTAAAMCATTSRPKSPFFSSNYPQKTSFTAVSLHRLNKLPTSGLRFS---- 60
           MALDNCIQLSTAAAM  TTS PKS  FSS + QKT+   +SL RLNK PTS  RF     
Sbjct: 1   MALDNCIQLSTAAAMYGTTSIPKSHPFSSTFHQKTT---LSLQRLNKFPTSIARFKSFSP 60

Query: 61  SPSSSTFKKSFSITCQARGAVDDVKEVTESSWNNLVVENQKAVLVEFWAPWCGPCKIIEP 120
           S S STF KS S+TC+ARGAVDDVKEVTESSWN LVVE++K VLVEFWAPWCGPCK+IEP
Sbjct: 61  STSVSTFGKSRSVTCKARGAVDDVKEVTESSWNELVVESEKTVLVEFWAPWCGPCKMIEP 120

Query: 121 VIKELAQEYAGKIVCLKLNTDISPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPKSTLA 180
           VI+ELAQ+YAGKIVCLKLNTD SPNVASKYGIRSIPTVLFFKNG+KRE VIGAVPKSTL+
Sbjct: 121 VIRELAQDYAGKIVCLKLNTDASPNVASKYGIRSIPTVLFFKNGQKREGVIGAVPKSTLS 180

Query: 181 ATIDKYVEV 186
           A+IDKY+EV
Sbjct: 181 ASIDKYLEV 186

[Close]

BLAST of Lsi08G005140 vs. ExPASy TrEMBL
Match: A0A6J1GXN7 (uncharacterized protein LOC111457772 OS=Cucurbita moschata OX=3662 GN=LOC111457772 PE=4 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 4.1e-72
Identity = 147/189 (77.78%), Postives = 161/189 (85.19%), Query Frame = 0


Query: 1   MALDNCIQLSTAAAMCATTSRPKSPFFSSNYPQKTSFTAVSLHRLNKLPTSGLRFS---- 60
           MALDNCIQLSTAAAM  TTS PKS  FSS + QK +   +SL RLNK PTS  RF     
Sbjct: 1   MALDNCIQLSTAAAMYGTTSIPKSHPFSSTFHQKPT---LSLQRLNKFPTSTARFKSFSP 60

Query: 61  SPSSSTFKKSFSITCQARGAVDDVKEVTESSWNNLVVENQKAVLVEFWAPWCGPCKIIEP 120
           S S STF KS S+TC+ARGAVDDVKEVTESSWN LVVE++K VLVEFWAPWCGPCK+IEP
Sbjct: 61  STSVSTFGKSRSVTCKARGAVDDVKEVTESSWNELVVESEKTVLVEFWAPWCGPCKMIEP 120

Query: 121 VIKELAQEYAGKIVCLKLNTDISPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPKSTLA 180
           VI+ELAQ+YAGKIVCLKLNTD SPNVASKYGIRSIPTVLFFKNG+KRE VIGAVPKSTL+
Sbjct: 121 VIRELAQDYAGKIVCLKLNTDASPNVASKYGIRSIPTVLFFKNGQKREGVIGAVPKSTLS 180

Query: 181 ATIDKYVEV 186
           A+IDKY+EV
Sbjct: 181 ASIDKYLEV 186

[Close]

BLAST of Lsi08G005140 vs. NCBI nr
Match: XP_038884238.1 (thioredoxin M-type, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 325.9 bits (834), Expect = 2.3e-85
Identity = 165/185 (89.19%), Postives = 172/185 (92.97%), Query Frame = 0


Query: 1   MALDNCIQLSTAAAMCATTSRPKSPFFSSNYPQKTSFTAVSLHRLNKLPTSGLRFSSPSS 60
           MALDNCIQLSTAAAMCATTSRPKS  F SN+PQKTSF+       NKLPT  LRFS+PSS
Sbjct: 1   MALDNCIQLSTAAAMCATTSRPKSHLFPSNFPQKTSFS-------NKLPTLRLRFSTPSS 60

Query: 61  STFKKSFSITCQARGAVDDVKEVTESSWNNLVVENQKAVLVEFWAPWCGPCKIIEPVIKE 120
           STF+KSFS+TCQARGAVDDVKEVTESSWNNLVVE+QKAVLVEFWAPWCGPCKIIEPVIKE
Sbjct: 61  STFRKSFSVTCQARGAVDDVKEVTESSWNNLVVESQKAVLVEFWAPWCGPCKIIEPVIKE 120

Query: 121 LAQEYAGKIVCLKLNTDISPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPKSTLAATID 180
           LA EYAGKIVCLKLNTD+SPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPKSTLAATID
Sbjct: 121 LAAEYAGKIVCLKLNTDVSPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPKSTLAATID 178

Query: 181 KYVEV 186
           KYVEV
Sbjct: 181 KYVEV 178

[Close]

BLAST of Lsi08G005140 vs. NCBI nr
Match: XP_004150725.2 (thioredoxin M-type, chloroplastic [Cucumis sativus] >KAE8647190.1 hypothetical protein Csa_019038 [Cucumis sativus])

HSP 1 Score: 302.4 bits (773), Expect = 2.7e-78
Identity = 154/186 (82.80%), Postives = 162/186 (87.10%), Query Frame = 0


Query: 1   MALDNCIQLSTAAAMCATTSRPKSPFFSS--NYPQKTSFTAVSLHRLNKLPTSGLRFSSP 60
           MALDNCIQLSTAA MCA  S PKS +FSS  NYPQKTSF+ +SL RLNK PT        
Sbjct: 1   MALDNCIQLSTAATMCAAASIPKSHYFSSTTNYPQKTSFSTLSLQRLNKSPT-------- 60

Query: 61  SSSTFKKSFSITCQARGAVDDVKEVTESSWNNLVVENQKAVLVEFWAPWCGPCKIIEPVI 120
              TF+K FS+TCQARGAVDDVKEVTESSWNNLVVENQKAVLVEFWAPWCGPCKIIEPVI
Sbjct: 61  --PTFRKPFSLTCQARGAVDDVKEVTESSWNNLVVENQKAVLVEFWAPWCGPCKIIEPVI 120

Query: 121 KELAQEYAGKIVCLKLNTDISPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPKSTLAAT 180
           KELA EYAGKIVCLKLNTD+SPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPKSTL A+
Sbjct: 121 KELAAEYAGKIVCLKLNTDLSPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPKSTLTAS 176

Query: 181 IDKYVE 185
           IDKY+E
Sbjct: 181 IDKYIE 176

[Close]

BLAST of Lsi08G005140 vs. NCBI nr
Match: XP_016899386.1 (PREDICTED: thioredoxin-like [Cucumis melo] >KAA0058857.1 thioredoxin-like [Cucumis melo var. makuwa] >TYK23765.1 thioredoxin-like [Cucumis melo var. makuwa])

HSP 1 Score: 300.4 bits (768), Expect = 1.0e-77
Identity = 153/187 (81.82%), Postives = 162/187 (86.63%), Query Frame = 0


Query: 1   MALDNCIQLSTAAAMCATTSRPKSPFFSS--NYPQKTSFTAVSLHRLNKLPTSGLRFSSP 60
           MALDNCIQLSTAA MCA  S PKS +FSS  N+PQK SF+ +SL RLNK PT        
Sbjct: 1   MALDNCIQLSTAATMCAAASIPKSHYFSSTANFPQKLSFSTLSLQRLNKFPT-------- 60

Query: 61  SSSTFKKSFSITCQARGAVDDVKEVTESSWNNLVVENQKAVLVEFWAPWCGPCKIIEPVI 120
              TF+K FS+TCQARGAVDDVKEVTESSWNNLVVENQKAVLVEFWAPWCGPCKIIEPVI
Sbjct: 61  --PTFRKPFSVTCQARGAVDDVKEVTESSWNNLVVENQKAVLVEFWAPWCGPCKIIEPVI 120

Query: 121 KELAQEYAGKIVCLKLNTDISPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPKSTLAAT 180
           KELA EYAGKI CLKLNTD+SPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPKSTLAA+
Sbjct: 121 KELAAEYAGKIECLKLNTDVSPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPKSTLAAS 177

Query: 181 IDKYVEV 186
           IDKY+EV
Sbjct: 181 IDKYIEV 177

[Close]

BLAST of Lsi08G005140 vs. NCBI nr
Match: XP_022989806.1 (uncharacterized protein LOC111486882 [Cucurbita maxima])

HSP 1 Score: 282.3 bits (721), Expect = 2.9e-72
Identity = 148/189 (78.31%), Postives = 162/189 (85.71%), Query Frame = 0


Query: 1   MALDNCIQLSTAAAMCATTSRPKSPFFSSNYPQKTSFTAVSLHRLNKLPTSGLRFS---- 60
           MALDNCIQLSTAAAM  TTS PKS  FSS + QKT+   +SL RLNK PTS  RF     
Sbjct: 1   MALDNCIQLSTAAAMYGTTSIPKSHPFSSTFHQKTT---LSLQRLNKFPTSIARFKSFSP 60

Query: 61  SPSSSTFKKSFSITCQARGAVDDVKEVTESSWNNLVVENQKAVLVEFWAPWCGPCKIIEP 120
           S S STF KS S+TC+ARGAVDDVKEVTESSWN LVVE++K VLVEFWAPWCGPCK+IEP
Sbjct: 61  STSVSTFGKSRSVTCKARGAVDDVKEVTESSWNELVVESEKTVLVEFWAPWCGPCKMIEP 120

Query: 121 VIKELAQEYAGKIVCLKLNTDISPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPKSTLA 180
           VI+ELAQ+YAGKIVCLKLNTD SPNVASKYGIRSIPTVLFFKNG+KRE VIGAVPKSTL+
Sbjct: 121 VIRELAQDYAGKIVCLKLNTDASPNVASKYGIRSIPTVLFFKNGQKREGVIGAVPKSTLS 180

Query: 181 ATIDKYVEV 186
           A+IDKY+EV
Sbjct: 181 ASIDKYLEV 186

[Close]

BLAST of Lsi08G005140 vs. NCBI nr
Match: KAG7032842.1 (hypothetical protein SDJN02_06892 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 280.8 bits (717), Expect = 8.5e-72
Identity = 147/189 (77.78%), Postives = 162/189 (85.71%), Query Frame = 0


Query: 1   MALDNCIQLSTAAAMCATTSRPKSPFFSSNYPQKTSFTAVSLHRLNKLPTSGLRFSSPSS 60
           MALDNCIQLSTAAAM  TTS PKS  FSS + QK +   +SL RLNK PTS  RF S S+
Sbjct: 1   MALDNCIQLSTAAAMYGTTSIPKSHPFSSTFHQKPT---LSLQRLNKFPTSTARFKSFST 60

Query: 61  ST----FKKSFSITCQARGAVDDVKEVTESSWNNLVVENQKAVLVEFWAPWCGPCKIIEP 120
           ST    F KS S+TC+ARGAVDDVKEVTESSWN LVVE++K VLVEFWAPWCGPCK+IEP
Sbjct: 61  STSVSSFGKSRSVTCKARGAVDDVKEVTESSWNELVVESEKTVLVEFWAPWCGPCKMIEP 120

Query: 121 VIKELAQEYAGKIVCLKLNTDISPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPKSTLA 180
           VI+ELAQ+YAGKIVCLKLNTD SPNVASKYGIRSIPTVLFFKNG+KRE VIGAVPKSTL+
Sbjct: 121 VIRELAQDYAGKIVCLKLNTDASPNVASKYGIRSIPTVLFFKNGQKREGVIGAVPKSTLS 180

Query: 181 ATIDKYVEV 186
           A+IDKY+EV
Sbjct: 181 ASIDKYLEV 186

[Close]

BLAST of Lsi08G005140 vs. TAIR 10
Match: AT4G03520.1 (Thioredoxin superfamily protein )

HSP 1 Score: 147.9 bits (372), Expect = 8.0e-36
Identity = 64/140 (45.71%), Postives = 100/140 (71.43%), Query Frame = 0


Query: 44  RLNKLPTSGLRFSSPSSSTFKKSFSITCQARGAVDDVKEVTESSWNNLVVENQKAVLVEF 103
           RL+  P S      P  S  ++  ++ C+A+    D++ V +S+W++LV++    V+V+F
Sbjct: 48  RLSLSPASLTSIHQPRVSRLRR--AVVCEAQETTTDIQVVNDSTWDSLVLKATGPVVVDF 107

Query: 104 WAPWCGPCKIIEPVIKELAQEYAGKIVCLKLNTDISPNVASKYGIRSIPTVLFFKNGEKR 163
           WAPWCGPCK+I+P++ +LAQ Y GKI   KLNTD SPN   +YG+RSIPT++ F  GEK+
Sbjct: 108 WAPWCGPCKMIDPLVNDLAQHYTGKIKFYKLNTDESPNTPGQYGVRSIPTIMIFVGGEKK 167

Query: 164 ESVIGAVPKSTLAATIDKYV 184
           +++IGAVPK+TL +++DK++
Sbjct: 168 DTIIGAVPKTTLTSSLDKFL 185

[Close]

BLAST of Lsi08G005140 vs. TAIR 10
Match: AT3G15360.1 (thioredoxin M-type 4 )

HSP 1 Score: 142.1 bits (357), Expect = 4.4e-34
Identity = 60/118 (50.85%), Postives = 89/118 (75.42%), Query Frame = 0


Query: 69  ITCQAR---GAVDDVKEVTESSWNNLVVENQKAVLVEFWAPWCGPCKIIEPVIKELAQEY 128
           I C+A+    A  +V  +++S W   V+E+   VLVEFWAPWCGPC++I P++ +LA+++
Sbjct: 74  IACEAQDTTAAAVEVPNLSDSEWQTKVLESDVPVLVEFWAPWCGPCRMIHPIVDQLAKDF 133

Query: 129 AGKIVCLKLNTDISPNVASKYGIRSIPTVLFFKNGEKRESVIGAVPKSTLAATIDKYV 184
           AGK    K+NTD SPN A++YGIRS+PTV+ FK GEK++S+IGAVP+ TL  TI++++
Sbjct: 134 AGKFKFYKINTDESPNTANRYGIRSVPTVIIFKGGEKKDSIIGAVPRETLEKTIERFL 191

[Close]

BLAST of Lsi08G005140 vs. TAIR 10
Match: AT1G03680.1 (thioredoxin M-type 1 )

HSP 1 Score: 141.0 bits (354), Expect = 9.8e-34
Identity = 65/140 (46.43%), Postives = 96/140 (68.57%), Query Frame = 0


Query: 50  TSGLRFSSPSSSTFKKS------FSITCQARGAVDDVKEVTESSWNNLVVENQKAVLVEF 109
           +SGLR     SS  K S        + C+A+     +  V +S+W++LV++  + V V+F
Sbjct: 40  SSGLRTRVSLSSLSKNSRVSRLRRGVICEAQDTATGIPVVNDSTWDSLVLKADEPVFVDF 99

Query: 110 WAPWCGPCKIIEPVIKELAQEYAGKIVCLKLNTDISPNVASKYGIRSIPTVLFFKNGEKR 169
           WAPWCGPCK+I+P++ ELAQ+YAG+    KLNTD SP    +YG+RSIPT++ F NGEK+
Sbjct: 100 WAPWCGPCKMIDPIVNELAQKYAGQFKFYKLNTDESPATPGQYGVRSIPTIMIFVNGEKK 159

Query: 170 ESVIGAVPKSTLAATIDKYV 184
           +++IGAV K TLA +I+K++
Sbjct: 160 DTIIGAVSKDTLATSINKFL 179

[Close]

BLAST of Lsi08G005140 vs. TAIR 10
Match: AT2G15570.2 (Thioredoxin superfamily protein )

HSP 1 Score: 111.3 bits (277), Expect = 8.3e-25
Identity = 57/135 (42.22%), Postives = 84/135 (62.22%), Query Frame = 0


Query: 53  LRFS------SPSSSTFKKSFSITCQARGAVDDVKEVTESSWNNLVVENQKAVLVEFWAP 112
           LRFS      S S+S  + S      +R A     EVT+ SW + V++++  VLVEF+  
Sbjct: 40  LRFSDRRSLLSSSASRLRLSPLCVRDSRAAA----EVTQRSWEDSVLKSETPVLVEFYTS 99

Query: 113 WCGPCKIIEPVIKELAQEYAGKIVCLKLNTDISPNVASKYGIRSIPTVLFFKNGEKRESV 172
           WCGPC+++  +I E+A +YAGK+ C  LN D    VA +Y I+++P VL FKNGEKRES+
Sbjct: 100 WCGPCRMVHRIIDEIAGDYAGKLNCYLLNADNDLPVAEEYEIKAVPVVLLFKNGEKRESI 159

Query: 173 IGAVPKSTLAATIDK 182
           +G +PK    + I++
Sbjct: 160 MGTMPKEFYISAIER 170

[Close]

BLAST of Lsi08G005140 vs. TAIR 10
Match: AT2G15570.1 (Thioredoxin superfamily protein )

HSP 1 Score: 110.9 bits (276), Expect = 1.1e-24
Identity = 57/135 (42.22%), Postives = 84/135 (62.22%), Query Frame = 0


Query: 53  LRFS------SPSSSTFKKSFSITCQARGAVDDVKEVTESSWNNLVVENQKAVLVEFWAP 112
           LRFS      S S+S  + S      +R A     EVT+ SW + V++++  VLVEF+  
Sbjct: 40  LRFSDRRSLLSSSASRLRLSPLCVRDSRAA-----EVTQRSWEDSVLKSETPVLVEFYTS 99

Query: 113 WCGPCKIIEPVIKELAQEYAGKIVCLKLNTDISPNVASKYGIRSIPTVLFFKNGEKRESV 172
           WCGPC+++  +I E+A +YAGK+ C  LN D    VA +Y I+++P VL FKNGEKRES+
Sbjct: 100 WCGPCRMVHRIIDEIAGDYAGKLNCYLLNADNDLPVAEEYEIKAVPVVLLFKNGEKRESI 159

Query: 173 IGAVPKSTLAATIDK 182
           +G +PK    + I++
Sbjct: 160 MGTMPKEFYISAIER 169

The following BLAST results are available for this feature:

BLAST of Lsi08G005140 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P07591	8.6e-43	48.69	Thioredoxin M-type, chloroplastic OS=Spinacia oleracea OX=3562 PE=1 SV=2	[more]
Q9ZP20	3.6e-41	60.77	Thioredoxin M5, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=TRXM P...	[more]
Q41864	6.2e-41	68.14	Thioredoxin M-type, chloroplastic OS=Zea mays OX=4577 GN=TRM1 PE=2 SV=1	[more]
P48384	6.2e-41	64.35	Thioredoxin M-type, chloroplastic OS=Pisum sativum OX=3888 PE=2 SV=1	[more]
Q9ZP21	8.4e-38	44.92	Thioredoxin M-type, chloroplastic OS=Triticum aestivum OX=4565 PE=2 SV=1	[more]

BLAST of Lsi08G005140 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KIC1	5.9e-79	82.89	Thioredoxin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G343710...	[more]
A0A5D3DJJ9	5.0e-78	81.82	Thioredoxin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1607G00...	[more]
A0A1S4DTR8	5.0e-78	81.82	thioredoxin-like OS=Cucumis melo OX=3656 GN=LOC107990489 PE=4 SV=1	[more]
A0A6J1JL70	1.4e-72	78.31	uncharacterized protein LOC111486882 OS=Cucurbita maxima OX=3661 GN=LOC111486882...	[more]
A0A6J1GXN7	4.1e-72	77.78	uncharacterized protein LOC111457772 OS=Cucurbita moschata OX=3662 GN=LOC1114577...	[more]

BLAST of Lsi08G005140 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038884238.1	2.3e-85	89.19	thioredoxin M-type, chloroplastic-like [Benincasa hispida]	[more]
XP_004150725.2	2.7e-78	82.80	thioredoxin M-type, chloroplastic [Cucumis sativus] >KAE8647190.1 hypothetical p...	[more]
XP_016899386.1	1.0e-77	81.82	PREDICTED: thioredoxin-like [Cucumis melo] >KAA0058857.1 thioredoxin-like [Cucum...	[more]
XP_022989806.1	2.9e-72	78.31	uncharacterized protein LOC111486882 [Cucurbita maxima]	[more]
KAG7032842.1	8.5e-72	77.78	hypothetical protein SDJN02_06892 [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of Lsi08G005140 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G03520.1	8.0e-36	45.71	Thioredoxin superfamily protein	[more]
AT3G15360.1	4.4e-34	50.85	thioredoxin M-type 4	[more]
AT1G03680.1	9.8e-34	46.43	thioredoxin M-type 1	[more]
AT2G15570.2	8.3e-25	42.22	Thioredoxin superfamily protein	[more]
AT2G15570.1	1.1e-24	42.22	Thioredoxin superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00421	THIOREDOXIN	coord: 147..158 score: 44.63 coord: 107..116 score: 63.28 coord: 99..107 score: 64.57
None	No IPR available	GENE3D	3.40.30.10	Glutaredoxin	coord: 79..184 e-value: 4.7E-35 score: 121.8
None	No IPR available	PANTHER	PTHR45663	GEO12009P1	coord: 28..182
None	No IPR available	PANTHER	PTHR45663:SF34	THIOREDOXIN M-TYPE PROTEIN	coord: 28..182
None	No IPR available	CDD	cd02947	TRX_family	coord: 92..180 e-value: 1.96871E-37 score: 122.667
IPR013766	Thioredoxin domain	PFAM	PF00085	Thioredoxin	coord: 80..181 e-value: 3.2E-32 score: 110.6
IPR013766	Thioredoxin domain	PROSITE	PS51352	THIOREDOXIN_2	coord: 58..184 score: 14.619746
IPR005746	Thioredoxin	TIGRFAM	TIGR01068	TIGR01068	coord: 84..182 e-value: 4.3E-36 score: 121.1
IPR017937	Thioredoxin, conserved site	PROSITE	PS00194	THIOREDOXIN_1	coord: 100..118
IPR036249	Thioredoxin-like superfamily	SUPERFAMILY	52833	Thioredoxin-like	coord: 70..183

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi08G005140.1	Lsi08G005140.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006662	glycerol ether metabolic process
biological_process	GO:0042744	hydrogen peroxide catabolic process
biological_process	GO:0009657	plastid organization
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0016671	oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor
molecular_function	GO:0015035	protein-disulfide reductase activity