Lsi08G004970 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi08G004970
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionwall-associated receptor kinase 2-like
Locationchr08: 12840607 .. 12845993 (+)
RNA-Seq ExpressionLsi08G004970
SyntenyLsi08G004970
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CGATGGAATTTAAAAAATTTGGCAGTCCAATTTTCTAAAATTCTTGAGGTGAGGAGTCGATTCTTTGGGAGTCCGAGATCGATCTCCAATATATTTTATTAAAATTTTAATATGTTTTCTATATTTACTTAGTTCATCGTTATATTATGTTTTGATTTCTATTTGAAATTTATCCAATTTTTTATTTAAATCCTTTTCATAACTTTTTTTTTTTAAATCATTTAAATTTTTCTTACAACCTTTTGTTTTCTAACCTTTAAAAAATTAATCTATTATTTCATAAGGTTTCCTAATCAATTTTTTCTAATAACTTGTTTTCAGGACCGTTTTTCTTAAAAAAAATTAAAAAAATTCCTACAATGGAATCTAGAAATATCTAGGAGTCCGTTTTTCTAGATTCTTGAGGTGAGGGATCATATCTTTGGGAGTCCGAGATTTTGATCGCAAGAAAAAATGAACTTAATTAATGTTTTTTTTTAAAAAAATCCTTTTATTAAAAGCTATTCCTATGACGGATTTGAGAAATTGTAATACTTTCTCAATTTCTCGAGGTGAGGAATTTTTCCTCAATTCTAGTGTAGCCTAATTGATGGAAATATGTTCCACTTATGGGATCATTACTTCAAATATTAGAGTAATGAGGGGTAAAATAAGACATTTGTTTTTAAAAAGAAATCCTAAATGATTTAATTTAAAGAATAATTTTTAATTCAAGATAAAAAAATGAATTGGCCACCGTTTTCTAAACGGGTGTTGTGGGGTGCTAACACCTTCTCCATACACAAATGACTCCCGAAATCAACTTAGTTTTTGCAGACTATTTTTATTGATTTTTACTTAAAATTTGTTTATTTTATTTCGGTGTCCAATCATATCGTACAAAAGATTGGTGGCGACTCCCTTTTTATTTTCTTTTTAAATTAACCCTTTTTAGAGGACATCGGCCGCTCCGCATCGTCTTGGGCACGTGGCGACACACCCGTATTCACCATTTTTTTTTTAAAAAAAAACTGCCCCATGAATTCACACATCATATTCTAATTTGAAAAGATTATCTAGAGTATCATTTTATTCTCACCCAATTAAGAACACAAGCAAAAGCTTAAGATTGGTTATAAATATTCAAACACGTACGCACCAAGTCATCTCCATTGAATTAATTTCACAGTCTAAACAATGAGAAGTCTAGAAGAGAAACTTATTAAGCTCATAATTTTATCATCAGCAACTTTGTTTGTGGTGGCAGCAGCGGTGAAGGCGTCGTTTACAGGCTGCAACGAACAGTGTGGAAACTTGAAAATTCCATATCCGTTCGGGATCAAGGAAGGGTGTTATCATAACGAAAATTTCTCAATTACTTGCAACAAAACTCGTTATAATCCACCAAAAGCATTTCTAAAAGACACCGACATAGGTATCACCAATATATCCATAATCGAAGGCCAGCTCCCCATCTTGCAATCCATAGCCAAAGATTGCTACACAAAAAATGGTACTCCTGAATCCAACGATCCCACTTTCCAACTCTTCAATTTCACAATTTCCAACACCATGAACAAGTTCACAGTCATTGGCTGCGATACTTACGCTTTTATTTCTGGTGAACTTGAGGGAGAATCCTACATAAGTGGGTGCATGGCGTTATGTGGAAACAGTACTGAAACTATAAGAGATGGGTCGTGCTCTGGGAGTGGGTGCTGTCAGTTGGAGATTCCTAGAGGCCTAAAAAATTTGGACGTTGAGGTTAAAAGCTTCGACAATCATAGTAAGGTACTCAACTTCAATCCATGTGGGTATGCTTTTGTAATCCAACAAGACAAATTCAATTTCTCCACAAACTATATTCGTAATTTTACACAAAAGGAAGTTCCATTGGTGCTTGATTGGGCCATCCCAACCAATAAGTCTTGCTTAGAGCAGGCTAAGAACAAAAGCAATTGCAGTATATGTGGACTAAATACCAAAAGGATTAACTTTCTTGACGATGGATCTGAATATCGTTGCCAGTGTTTGGAAGGCTTTGAAGGAAATCCATATCTCCCTCAAGGTTGCCAAGGTAAATAATTAGTATATTTCTCCTTTTCCATATATATATATATATATATATTTTGAGTTTAACATGTGGTGGGGTGGTTTAAATCTCAAACCTTTTGGTCTATGTTATGTCGAGGATCTCATATTGAAAAGATGAGGAGATCTCGCATTGAGAATTTGGTCGAAAAATAAAAATTGGGATCTGATCCATAAATTGTGAACCCAAAAAAGCACATTTTGAGAGACACGTGTTGAAGATCGCACATTGAAAAAATCAAGGAAATCTCACTATGTTTATAAGATAGATGTAAAAGTACTACTCCTCTCATTACCTATTGATTTTGAGAAAGAACCTCAAATTATCTAAACCTCATATTTAGATATCAAAGCTCATGAATCCCTCAAAACAACCATTTGGTCTAAAAATATGTTGGGATCTGATTCAAGAATAGTGAACTTAAAGGGACAATCTTTATAAGATACGTGGGTTACTCTACTCATAGTCAATTAATTTTGAGATAAAACTTCATATTTATCTAATATAGTATAAATTTTATATAAGCTGAATTGTGATTATTTTGATTTTTTTTTTTTTTAACATATAGTGTAACGGACATTGATTTTGGCATTTTACAGATATAGATGAATGCAAGAATGGAAGCCAGAAATGTAAATACAAAGAGTTGTGCGTTAACACACCAGGAAACTATACTTGTCATTGTCCTAAGAACCATAAAGGAGATGGCAGATATGGAGGAGAAGGCTGCATTCCAAACTCCATCTCTTCAATTCATATCATCATTGGTGAGTTCTTATATTTTAGTACAAAATATATGTAGTATATTATCCGGAAAAATGTCTTTTTCCTCCCTTAGTTTGGATCATTTAGTTCCTAAATTATTACTTCTAAGTTTTGAACTTTGTTAGAACTTTGTTTTCAGTTGGTGTTTAAACTTTATAATGTTACATTTTAACCCTTCAATTTAATGTTTAGTTTTCATTTGGTCTAAGTTTCAAAATGTTAAATTTATAACTTTGAGTTTTGAATTGAAAAATATTTAGGTGCATCCCTAGGCAACACTTATTTTTGTGCATCCCACCAAATTGTCCGATCATAATTTGTCATGTGACAAATACTTTTTAATAATTAGGTAGAACGTAGACTACATCTAATCTCATGCATCCCATTTTCTATATATAACATGGTAAAAAAAAAGATAAAAGTAATTCAATTTTAAGAAAAAAAATATTGTCACATGACGGATTTCTATTAGGTGCTATCTAAGCTACACAAAAATGAGTGGAGCTATTTTTTTTTTTAAGTATTTTAATTCTTTATTGGTGTGAATAATGAAAATGAATGTAAAAAGAGGATGCACCCAAGTATTAACCTTTTGAATCCAATTTCCATTTAGTTCTAAGATTTCTAAGTTTTACACTTTTACCCCAAGTAATCAATTTTATTTTAATTAATTTTAAATAGTTATGATCTTAAATTTTCAATTAATTGTAATATAGTCCGAAGTTTAATTTATTGAAAACTAAGTTAATTATAATTTATTTGATGGTTTTAAATTAGTTAATAAAAAATTAATACTAAGTGGACATTAGTGAAAACTAGAGGTAAGAGTATACGATCTTGAAGTTTATAAACCAAATAGAAAATAAACTCAAAACTCAATGGTAAAAATGTAACATTTTGAAATTTAGAATGAAAAAAAAATTAAATTCAAAATTCGTGAATAAAAGTGTACCATTTTAAAATTTAAGAACCAAATAAAAATTACCATACTCAGGAAGAAACATATATATCTGATACGTACATTGATGAAATAAAATGCAGGAACTTGTGTGGGGTTCACAGTATTTGTGTTTGGCAGTATATGGTTATACTTGGGTTACAAAAAGTGGAAATTTATTCAACAAAAGGAGAAGTTCTTTAAGAAAAATGGAGGTTTAATGCTTCGGCAACATCTTTCTCAATGGCAATCACCAGACATGCTCAAAATTTTCACCCAAGAAGAGTTGGAGAAGGCTACAAACAAATATGACGAAAAAGCAGTGGTTGGCAAAGGTGGTTACGGTACCGTTTACAAAGGAGTCTTAGACAACGGTTTGACAGTTGCAATCAAGAAATCAAAATTAGTGAACCAGTCCCAAATTGATCAATTTATTAACGAAGTCAACGTTCTGTCCCAAATCAACCATCGCAACGTCGTCAAACTCATATGGTGTTGTTTGGAGACAAAGGTTCCATTGTTGGTGTACGAGCTCGTAACGAACGGCACACTTTTTGAACACATCCACCGCAAAACCAACCAAGTTTATCTTCCATGGGAAGCTCGCTTGAAAATAGCTTCGGAAATTGCCAGTGTAGTTTCATATTTGCATTCTTCCGCTTCTACTCCAATTATTCATAGAGATATCAAGTCCAGTAATATACTTTTGGACCAAAACTACACAGCAAAGATCTCTGATTTTGGAACGTCAAAGCTTGTTCCGTTGGATCGAACTCAAATATCCACAATGGTGCAAGGGACTATTGGATATTTGGATCCAGAATATTTTTTGACGAGTGAGTTGACGGAGAAAAGCGATGTGTACAGCTTTGGAATTGTGCTTATCACTACAAGAAAGTTGACTATTTTCGAAGGTCACAACCTTCGAAAAAACCTTCAATAAACCTTCGATAAACTTTTTTTCAAAGGTTATCTCAACCTTTGAAAAGTGTCGAAATAGATCCTGTTGAAAAAACTTTTCCAAAGGTTTACTAACCTTCGAAAATTATTTTTTTTCAAAGGTTTATTAACCTTAAAAAATTGTTGTTTTTCAAAGGTCACAAGTCTTCGATAATTATCAAATTATTTGTCGATAATTATATATTGGCAAATGATTTTTTCGAAGGTTTTCATTATTTTTAAAGGCTCTATTTGAAAGATTTTAAAGCCTCATAAAAACTTCTTTATCAACAATTATAAATCCTTCGAAAATTCTCTCGCAAAAACTTTTAGCATTTAAATTATTACTGACAAATCTCAAGAAGGTTGTTTTTACTAATTGACCCACCTATAATATCTTGAATTACAAAACAGAAGAATGGAATAGAAAAAATAGGCAAAGATATTGATTAAAGTAAACTTAGATGTCATAATGTTCAAAACCCTTTATTCCAAATGCTTCTGGTCTACATTTTTACATTTTTCAGTCCACACTGTTTTTTACTCTCAATCTATTAGTTCATTTTCTACCAAATGAGCCTTTTCCTATCATGTTATGATGTGAAAGACGACGATGACAATATCGACAATACGAAAGATAAGGCAGTTGATCACAATAAAGCAATTCAAAAGTTA

mRNA sequence

CGATGGAATTTAAAAAATTTGGCAGTCCAATTTTCTAAAATTCTTGAGGTGAGGAGTCGATTCTTTGGGAGTCCGAGATCGATCTCCAATATATTTTATTAAAATTTTAATATGTTTTCTATATTTACTTAGTTCATCGTTATATTATGTTTTGATTTCTATTTGAAATTTATCCAATTTTTTATTTAAATCCTTTTCATAACTTTTTTTTTTTAAATCATTTAAATTTTTCTTACAACCTTTTGTTTTCTAACCTTTAAAAAATTAATCTATTATTTCATAAGGTTTCCTAATCAATTTTTTCTAATAACTTGTTTTCAGGACCGTTTTTCTTAAAAAAAATTAAAAAAATTCCTACAATGGAATCTAGAAATATCTAGGAGTCCGTTTTTCTAGATTCTTGAGGTGAGGGATCATATCTTTGGGAGTCCGAGATTTTGATCGCAAGAAAAAATGAACTTAATTAATGTTTTTTTTTAAAAAAATCCTTTTATTAAAAGCTATTCCTATGACGGATTTGAGAAATTGTAATACTTTCTCAATTTCTCGAGGTGAGGAATTTTTCCTCAATTCTAGTGTAGCCTAATTGATGGAAATATGTTCCACTTATGGGATCATTACTTCAAATATTAGAGTAATGAGGGGTAAAATAAGACATTTGTTTTTAAAAAGAAATCCTAAATGATTTAATTTAAAGAATAATTTTTAATTCAAGATAAAAAAATGAATTGGCCACCGTTTTCTAAACGGGTGTTGTGGGGTGCTAACACCTTCTCCATACACAAATGACTCCCGAAATCAACTTAGTTTTTGCAGACTATTTTTATTGATTTTTACTTAAAATTTGTTTATTTTATTTCGGTGTCCAATCATATCGTACAAAAGATTGGTGGCGACTCCCTTTTTATTTTCTTTTTAAATTAACCCTTTTTAGAGGACATCGGCCGCTCCGCATCGTCTTGGGCACGTGGCGACACACCCGTATTCACCATTTTTTTTTTAAAAAAAAACTGCCCCATGAATTCACACATCATATTCTAATTTGAAAAGATTATCTAGAGTATCATTTTATTCTCACCCAATTAAGAACACAAGCAAAAGCTTAAGATTGGTTATAAATATTCAAACACGTACGCACCAAGTCATCTCCATTGAATTAATTTCACAGTCTAAACAATGAGAAGTCTAGAAGAGAAACTTATTAAGCTCATAATTTTATCATCAGCAACTTTGTTTGTGGTGGCAGCAGCGGTGAAGGCGTCGTTTACAGGCTGCAACGAACAGTGTGGAAACTTGAAAATTCCATATCCGTTCGGGATCAAGGAAGGGTGTTATCATAACGAAAATTTCTCAATTACTTGCAACAAAACTCGTTATAATCCACCAAAAGCATTTCTAAAAGACACCGACATAGGTATCACCAATATATCCATAATCGAAGGCCAGCTCCCCATCTTGCAATCCATAGCCAAAGATTGCTACACAAAAAATGGTACTCCTGAATCCAACGATCCCACTTTCCAACTCTTCAATTTCACAATTTCCAACACCATGAACAAGTTCACAGTCATTGGCTGCGATACTTACGCTTTTATTTCTGGTGAACTTGAGGGAGAATCCTACATAAGTGGGTGCATGGCGTTATGTGGAAACAGTACTGAAACTATAAGAGATGGGTCGTGCTCTGGGAGTGGGTGCTGTCAGTTGGAGATTCCTAGAGGCCTAAAAAATTTGGACGTTGAGGTTAAAAGCTTCGACAATCATAGTAAGGTACTCAACTTCAATCCATGTGGGTATGCTTTTGTAATCCAACAAGACAAATTCAATTTCTCCACAAACTATATTCGTAATTTTACACAAAAGGAAGTTCCATTGGTGCTTGATTGGGCCATCCCAACCAATAAGTCTTGCTTAGAGCAGGCTAAGAACAAAAGCAATTGCAGTATATGTGGACTAAATACCAAAAGGATTAACTTTCTTGACGATGGATCTGAATATCGTTGCCAGTGTTTGGAAGGCTTTGAAGGAAATCCATATCTCCCTCAAGGTTGCCAAGATATAGATGAATGCAAGAATGGAAGCCAGAAATGTAAATACAAAGAGTTGTGCGTTAACACACCAGGAAACTATACTTGTCATTGTCCTAAGAACCATAAAGGAGATGGCAGATATGGAGGAGAAGGCTGCATTCCAAACTCCATCTCTTCAATTCATATCATCATTGTATTTGTGTTTGGCAGTATATGGTTATACTTGGGTTACAAAAAGTGGAAATTTATTCAACAAAAGGAGAAGTTCTTTAAGAAAAATGGAGGTTTAATGCTTCGGCAACATCTTTCTCAATGGCAATCACCAGACATGCTCAAAATTTTCACCCAAGAAGAGTTGGAGAAGGCTACAAACAAATATGACGAAAAAGCAGTGGTTGGCAAAGGTGGTTACGGTACCGTTTACAAAGGAGTCTTAGACAACGGTTTGACAGTTGCAATCAAGAAATCAAAATTAGTGAACCAGTCCCAAATTGATCAATTTATTAACGAAGTCAACGTTCTGTCCCAAATCAACCATCGCAACGTCGTCAAACTCATATGGTGTTGTTTGGAGACAAAGGTTCCATTGTTGGTGTACGAGCTCGTAACGAACGGCACACTTTTTGAACACATCCACCGCAAAACCAACCAAGTTTATCTTCCATGGGAAGCTCGCTTGAAAATAGCTTCGGAAATTGCCAGTGTAGTTTCATATTTGCATTCTTCCGCTTCTACTCCAATTATTCATAGAGATATCAAGTCCAGTAATATACTTTTGGACCAAAACTACACAGCAAAGATCTCTGATTTTGGAACGTCAAAGCTTGTTCCGTTGGATCGAACTCAAATATCCACAATGGTGCAAGGGACTATTGGATATTTGGATCCAGAATATTTTTTGACGAACGACGATGACAATATCGACAATACGAAAGATAAGGCAGTTGATCACAATAAAGCAATTCAAAAGTTA

Coding sequence (CDS)

ATGAGAAGTCTAGAAGAGAAACTTATTAAGCTCATAATTTTATCATCAGCAACTTTGTTTGTGGTGGCAGCAGCGGTGAAGGCGTCGTTTACAGGCTGCAACGAACAGTGTGGAAACTTGAAAATTCCATATCCGTTCGGGATCAAGGAAGGGTGTTATCATAACGAAAATTTCTCAATTACTTGCAACAAAACTCGTTATAATCCACCAAAAGCATTTCTAAAAGACACCGACATAGGTATCACCAATATATCCATAATCGAAGGCCAGCTCCCCATCTTGCAATCCATAGCCAAAGATTGCTACACAAAAAATGGTACTCCTGAATCCAACGATCCCACTTTCCAACTCTTCAATTTCACAATTTCCAACACCATGAACAAGTTCACAGTCATTGGCTGCGATACTTACGCTTTTATTTCTGGTGAACTTGAGGGAGAATCCTACATAAGTGGGTGCATGGCGTTATGTGGAAACAGTACTGAAACTATAAGAGATGGGTCGTGCTCTGGGAGTGGGTGCTGTCAGTTGGAGATTCCTAGAGGCCTAAAAAATTTGGACGTTGAGGTTAAAAGCTTCGACAATCATAGTAAGGTACTCAACTTCAATCCATGTGGGTATGCTTTTGTAATCCAACAAGACAAATTCAATTTCTCCACAAACTATATTCGTAATTTTACACAAAAGGAAGTTCCATTGGTGCTTGATTGGGCCATCCCAACCAATAAGTCTTGCTTAGAGCAGGCTAAGAACAAAAGCAATTGCAGTATATGTGGACTAAATACCAAAAGGATTAACTTTCTTGACGATGGATCTGAATATCGTTGCCAGTGTTTGGAAGGCTTTGAAGGAAATCCATATCTCCCTCAAGGTTGCCAAGATATAGATGAATGCAAGAATGGAAGCCAGAAATGTAAATACAAAGAGTTGTGCGTTAACACACCAGGAAACTATACTTGTCATTGTCCTAAGAACCATAAAGGAGATGGCAGATATGGAGGAGAAGGCTGCATTCCAAACTCCATCTCTTCAATTCATATCATCATTGTATTTGTGTTTGGCAGTATATGGTTATACTTGGGTTACAAAAAGTGGAAATTTATTCAACAAAAGGAGAAGTTCTTTAAGAAAAATGGAGGTTTAATGCTTCGGCAACATCTTTCTCAATGGCAATCACCAGACATGCTCAAAATTTTCACCCAAGAAGAGTTGGAGAAGGCTACAAACAAATATGACGAAAAAGCAGTGGTTGGCAAAGGTGGTTACGGTACCGTTTACAAAGGAGTCTTAGACAACGGTTTGACAGTTGCAATCAAGAAATCAAAATTAGTGAACCAGTCCCAAATTGATCAATTTATTAACGAAGTCAACGTTCTGTCCCAAATCAACCATCGCAACGTCGTCAAACTCATATGGTGTTGTTTGGAGACAAAGGTTCCATTGTTGGTGTACGAGCTCGTAACGAACGGCACACTTTTTGAACACATCCACCGCAAAACCAACCAAGTTTATCTTCCATGGGAAGCTCGCTTGAAAATAGCTTCGGAAATTGCCAGTGTAGTTTCATATTTGCATTCTTCCGCTTCTACTCCAATTATTCATAGAGATATCAAGTCCAGTAATATACTTTTGGACCAAAACTACACAGCAAAGATCTCTGATTTTGGAACGTCAAAGCTTGTTCCGTTGGATCGAACTCAAATATCCACAATGGTGCAAGGGACTATTGGATATTTGGATCCAGAATATTTTTTGACGAACGACGATGACAATATCGACAATACGAAAGATAAGGCAGTTGATCACAATAAAGCAATTCAAAAGTTA

Protein sequence

MRSLEEKLIKLIILSSATLFVVAAAVKASFTGCNEQCGNLKIPYPFGIKEGCYHNENFSITCNKTRYNPPKAFLKDTDIGITNISIIEGQLPILQSIAKDCYTKNGTPESNDPTFQLFNFTISNTMNKFTVIGCDTYAFISGELEGESYISGCMALCGNSTETIRDGSCSGSGCCQLEIPRGLKNLDVEVKSFDNHSKVLNFNPCGYAFVIQQDKFNFSTNYIRNFTQKEVPLVLDWAIPTNKSCLEQAKNKSNCSICGLNTKRINFLDDGSEYRCQCLEGFEGNPYLPQGCQDIDECKNGSQKCKYKELCVNTPGNYTCHCPKNHKGDGRYGGEGCIPNSISSIHIIIVFVFGSIWLYLGYKKWKFIQQKEKFFKKNGGLMLRQHLSQWQSPDMLKIFTQEELEKATNKYDEKAVVGKGGYGTVYKGVLDNGLTVAIKKSKLVNQSQIDQFINEVNVLSQINHRNVVKLIWCCLETKVPLLVYELVTNGTLFEHIHRKTNQVYLPWEARLKIASEIASVVSYLHSSASTPIIHRDIKSSNILLDQNYTAKISDFGTSKLVPLDRTQISTMVQGTIGYLDPEYFLTNDDDNIDNTKDKAVDHNKAIQKL
Homology
BLAST of Lsi08G004970 vs. ExPASy Swiss-Prot
Match: Q9LMP1 (Wall-associated receptor kinase 2 OS=Arabidopsis thaliana OX=3702 GN=WAK2 PE=1 SV=1)

HSP 1 Score: 472.6 bits (1215), Expect = 6.6e-132
Identity = 259/574 (45.12%), Postives = 370/574 (64.46%), Query Frame = 0

Query: 33  CNEQCGNLKIPYPFGIKEGCYH--NENFSITCNKTRYNPPKAFLKDTDIGITNISIIEGQ 92
           C  +CGN+ + YPFG   GCY+  +E+F++TCN+      K F    ++ + N+S + GQ
Sbjct: 29  CQTRCGNVAVEYPFGTSPGCYYPGDESFNLTCNEQE----KLFF--GNMPVINMS-LSGQ 88

Query: 93  LPILQSIAKDCYTKNGTPESNDPTFQ---LFNFTISNTMNKFTVIGCDTYAFI--SGELE 152
           L +    ++ CY   G  +  D   Q   L NFT+S  +N+FTV+GC++YAF+  SG   
Sbjct: 89  LRVRLVRSRVCYDSQG--KQTDYIAQRTTLGNFTLSE-LNRFTVVGCNSYAFLRTSGV-- 148

Query: 153 GESYISGCMALCGNSTETIRDGSCSGSGCCQLEIPRGLKNLDVEVKSFDNHSKVLNFNPC 212
            E Y +GC+++C ++  T ++GSCSG GCCQ+ +PRG   + V+  SF NH  V  FNPC
Sbjct: 149 -EKYSTGCISICDSA--TTKNGSCSGEGCCQIPVPRGYSFVRVKPHSFHNHPTVHLFNPC 208

Query: 213 GYAFVIQQDKFNF----STNYIRNFTQKEVPLVLDWAIPTNKSCLEQAKNKSNCSICGLN 272
            YAF+++   F+F      N +RN T    P+VLDW+I  +K+C    K      +CG N
Sbjct: 209 TYAFLVEDGMFDFHALEDLNNLRNVT--TFPVVLDWSI-GDKTC----KQVEYRGVCGGN 268

Query: 273 TKRINFLDDGSEYRCQCLEGFEGNPYLPQGCQDIDECKNGSQKCKYKELCVNTPGNYTCH 332
           +   +    G+ Y C+CLEGFEGNPYLP GCQDI+EC +    C     C NT G++ C+
Sbjct: 269 STCFD-STGGTGYNCKCLEGFEGNPYLPNGCQDINECISSRHNCSEHSTCENTKGSFNCN 328

Query: 333 CPKNHKGDGRYGGEGCI-PNSISSIHIIIVFVFGSIWLYLG-------YKKWKFIQQKEK 392
           CP  ++ D        + P       I +    G   + LG        K  K  + ++K
Sbjct: 329 CPSGYRKDSLNSCTRKVRPEYFRWTQIFLGTTIGFSVIMLGISCLQQKIKHRKNTELRQK 388

Query: 393 FFKKNGGLMLRQHLSQWQSPDM-LKIFTQEELEKATNKYDEKAVVGKGGYGTVYKGVLDN 452
           FF++NGG ML Q +S     ++ +KIFT++ +++ATN Y E  ++G+GG GTVYKG+L +
Sbjct: 389 FFEQNGGGMLIQRVSGAGPSNVDVKIFTEKGMKEATNGYHESRILGQGGQGTVYKGILPD 448

Query: 453 GLTVAIKKSKLVNQSQIDQFINEVNVLSQINHRNVVKLIWCCLETKVPLLVYELVTNGTL 512
              VAIKK++L N+SQ++QFINEV VLSQINHRNVVK++ CCLET+VPLLVYE + +GTL
Sbjct: 449 NSIVAIKKARLGNRSQVEQFINEVLVLSQINHRNVVKVLGCCLETEVPLLVYEFINSGTL 508

Query: 513 FEHIHRKTNQVYLPWEARLKIASEIASVVSYLHSSASTPIIHRDIKSSNILLDQNYTAKI 572
           F+H+H       L WE RL+IA+E+A  ++YLHSSAS PIIHRDIK++NILLD+N TAK+
Sbjct: 509 FDHLHGSLYDSSLTWEHRLRIATEVAGSLAYLHSSASIPIIHRDIKTANILLDKNLTAKV 568

Query: 573 SDFGTSKLVPLDRTQISTMVQGTIGYLDPEYFLT 587
           +DFG S+L+P+D+ Q++T+VQGT+GYLDPEY+ T
Sbjct: 569 ADFGASRLIPMDKEQLTTIVQGTLGYLDPEYYNT 579

BLAST of Lsi08G004970 vs. ExPASy Swiss-Prot
Match: Q39191 (Wall-associated receptor kinase 1 OS=Arabidopsis thaliana OX=3702 GN=WAK1 PE=1 SV=2)

HSP 1 Score: 449.1 bits (1154), Expect = 7.8e-125
Identity = 248/574 (43.21%), Postives = 355/574 (61.85%), Query Frame = 0

Query: 33  CNEQCGNLKIPYPFGIKEGCYH--NENFSITCNKTRYNPPKAFLKDTDIGITNISIIEGQ 92
           C  +CGN+ I YPFGI  GCY+  NE+FSITC + R +     L D ++   N S   GQ
Sbjct: 32  CQNKCGNITIEYPFGISSGCYYPGNESFSITCKEDRPH----VLSDIEVANFNHS---GQ 91

Query: 93  LPILQSIAKDCYTKNGTPESNDPTFQLFNFTISNTMNKFTVIGCDTYAFISGELEGESYI 152
           L +L + +  CY + G     D +F L N ++S   NK T +GC+  + +      ++Y 
Sbjct: 92  LQVLLNRSSTCYDEQGKKTEEDSSFTLENLSLS-ANNKLTAVGCNALSLLD-TFGMQNYS 151

Query: 153 SGCMALCGNSTETIRDGSCSGSGCCQLEIPRGLKNLDVEVKS--FDNHSKVLNFNPCGYA 212
           + C++LC +  E   DG C+G GCC++++   L +   E  S    + +   +F+PC YA
Sbjct: 152 TACLSLCDSPPEA--DGECNGRGCCRVDVSAPLDSYTFETTSGRIKHMTSFHDFSPCTYA 211

Query: 213 FVIQQDKFNFSTN----YIRNFTQKEVPLVLDWAIPTNKSCLEQAKNKSNCSICGLNTKR 272
           F+++ DKFNFS+      +RN  +   P++LDW++  N++C    +   + SICG N+  
Sbjct: 212 FLVEDDKFNFSSTEDLLNLRNVMR--FPVLLDWSV-GNQTC----EQVGSTSICGGNSTC 271

Query: 273 INFLDDGSEYRCQCLEGFEGNPYLPQGCQDIDECKNGS----QKCKYKELCVNTPGNYTC 332
           ++       Y C+C EGF+GNPYL  GCQD++EC   S      C   + C N  G + C
Sbjct: 272 LDSTPRNG-YICRCNEGFDGNPYLSAGCQDVNECTTSSTIHRHNCSDPKTCRNKVGGFYC 331

Query: 333 HCPKNHKGDGRYGGEGCIPNSISSIHIIIVFVFGSIWLYLG-------YKKWKFIQQKEK 392
            C   ++ D       C     +   I++V   G + + LG        K  K  + +E+
Sbjct: 332 KCQSGYRLDTT--TMSCKRKEFAWTTILLVTTIGFLVILLGVACIQQRMKHLKDTKLREQ 391

Query: 393 FFKKNGGLMLRQHLSQWQSPDM-LKIFTQEELEKATNKYDEKAVVGKGGYGTVYKGVLDN 452
           FF++NGG ML Q LS     ++ +KIFT++ ++KATN Y E  ++G+GG GTVYKG+L +
Sbjct: 392 FFEQNGGGMLTQRLSGAGPSNVDVKIFTEDGMKKATNGYAESRILGQGGQGTVYKGILPD 451

Query: 453 GLTVAIKKSKLVNQSQIDQFINEVNVLSQINHRNVVKLIWCCLETKVPLLVYELVTNGTL 512
              VAIKK++L + SQ++QFINEV VLSQINHRNVVKL+ CCLET+VPLLVYE +TNGTL
Sbjct: 452 NSIVAIKKARLGDSSQVEQFINEVLVLSQINHRNVVKLLGCCLETEVPLLVYEFITNGTL 511

Query: 513 FEHIHRKTNQVYLPWEARLKIASEIASVVSYLHSSASTPIIHRDIKSSNILLDQNYTAKI 572
           F+H+H       L WE RLKIA E+A  ++YLHSSAS PIIHRDIK++NILLD N TAK+
Sbjct: 512 FDHLHGSMIDSSLTWEHRLKIAIEVAGTLAYLHSSASIPIIHRDIKTANILLDVNLTAKV 571

Query: 573 SDFGTSKLVPLDRTQISTMVQGTIGYLDPEYFLT 587
           +DFG S+L+P+D+ ++ TMVQGT+GYLDPEY+ T
Sbjct: 572 ADFGASRLIPMDKEELETMVQGTLGYLDPEYYNT 584

BLAST of Lsi08G004970 vs. ExPASy Swiss-Prot
Match: Q9LMN8 (Wall-associated receptor kinase 3 OS=Arabidopsis thaliana OX=3702 GN=WAK3 PE=2 SV=2)

HSP 1 Score: 438.3 bits (1126), Expect = 1.4e-121
Identity = 247/580 (42.59%), Postives = 352/580 (60.69%), Query Frame = 0

Query: 33  CNEQCGNLKIPYPFGIKEGCYH--NENFSITCNKTRYNPPKAFLKDTDIGITNISIIEGQ 92
           C  +CGN+ I YPFGI  GCY+  ++NF++TC        +  L    I +TNIS   G 
Sbjct: 31  CKLKCGNVTIEYPFGISTGCYYPGDDNFNLTC-----VVEEKLLLFGIIQVTNIS-HSGH 90

Query: 93  LPILQSIAKDCYTKNGTPESNDPTFQLFNFTISNTMNKFTVIGCDTYAFISGELEGESYI 152
           + +L     +CY +          +QL +    ++ NKFT++GC+  + +S     ++Y 
Sbjct: 91  VSVLFERFSECYEQKNETNGTALGYQLGSSFSLSSNNKFTLVGCNALSLLS-TFGKQNYS 150

Query: 153 SGCMALCGNSTETIRDGSCSGSGCCQLE---IPRGLKNL---DVEVKSFDNHS------K 212
           +GC++LC +  E   +G C+G GCC  E   +P          V +++  N+S       
Sbjct: 151 TGCLSLCNSQPEA--NGRCNGVGCCTTEDFSVPFDSDTFQFGSVRLRNQVNNSLDLFNTS 210

Query: 213 VLNFNPCGYAFVIQQDKFNFSTN----YIRNFTQKEVPLVLDWAIPTNKSCLEQAKNKSN 272
           V  FNPC YAF+++  KFNF ++     +RN T+   P+ LDW+I  N++C EQA    +
Sbjct: 211 VYQFNPCTYAFLVEDGKFNFDSSKDLKNLRNVTR--FPVALDWSI-GNQTC-EQA---GS 270

Query: 273 CSICGLNTKRINFLDDGSEYRCQCLEGFEGNPYLPQGCQDIDECKNGSQKCKYKELCVNT 332
             ICG N+   N       Y C+C EG++GNPY  +GC+DIDEC + +  C   + C N 
Sbjct: 271 TRICGKNSSCYNSTTRNG-YICKCNEGYDGNPYRSEGCKDIDECISDTHNCSDPKTCRNR 330

Query: 333 PGNYTCHCPKNHKGDGRYGGEGCIPNSISSIHIIIVFVFGSIWLYL-------GYKKWKF 392
            G + C CP    G        C         I +V + G + L L         K+ K+
Sbjct: 331 DGGFDCKCP---SGYDLNSSMSCTRPEYKRTRIFLVIIIGVLVLLLAAICIQHATKQRKY 390

Query: 393 IQQKEKFFKKNGGLMLRQHLSQWQSPDM-LKIFTQEELEKATNKYDEKAVVGKGGYGTVY 452
            + + +FF++NGG ML Q LS     ++  KIFT+E +++ATN YDE  ++G+GG GTVY
Sbjct: 391 TKLRRQFFEQNGGGMLIQRLSGAGLSNIDFKIFTEEGMKEATNGYDESRILGQGGQGTVY 450

Query: 453 KGVLDNGLTVAIKKSKLVNQSQIDQFINEVNVLSQINHRNVVKLIWCCLETKVPLLVYEL 512
           KG+L +   VAIKK++L +  Q+DQFI+EV VLSQINHRNVVK++ CCLET+VPLLVYE 
Sbjct: 451 KGILPDNTIVAIKKARLADSRQVDQFIHEVLVLSQINHRNVVKILGCCLETEVPLLVYEF 510

Query: 513 VTNGTLFEHIHRKTNQVYLPWEARLKIASEIASVVSYLHSSASTPIIHRDIKSSNILLDQ 572
           +TNGTLF+H+H       L WE RL+IA E+A  ++YLHSSAS PIIHRDIK++NILLD+
Sbjct: 511 ITNGTLFDHLHGSIFDSSLTWEHRLRIAIEVAGTLAYLHSSASIPIIHRDIKTANILLDE 570

Query: 573 NYTAKISDFGTSKLVPLDRTQISTMVQGTIGYLDPEYFLT 587
           N TAK++DFG SKL+P+D+ Q++TMVQGT+GYLDPEY+ T
Sbjct: 571 NLTAKVADFGASKLIPMDKEQLTTMVQGTLGYLDPEYYTT 590

BLAST of Lsi08G004970 vs. ExPASy Swiss-Prot
Match: Q9LMN7 (Wall-associated receptor kinase 5 OS=Arabidopsis thaliana OX=3702 GN=WAK5 PE=2 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 1.2e-120
Identity = 243/580 (41.90%), Postives = 360/580 (62.07%), Query Frame = 0

Query: 33  CNEQCGNLKIPYPFGIKEGCYH--NENFSITCNKTRYNPPKAFLKDTDIGITNISII--- 92
           C  +CG++ I YPFGI  GCY+  +++F+ITC + + N            ++NI ++   
Sbjct: 29  CQTRCGDVPIDYPFGISTGCYYPGDDSFNITCEEDKPNV-----------LSNIEVLNFN 88

Query: 93  -EGQLPILQSIAKDCYTKNGTPESNDPTFQLFNFTISNTMNKFTVIGCDTYAFISGELEG 152
             GQL  L   +  CY +    +     F+L N + S   NKFT++GC+ +A +S     
Sbjct: 89  HSGQLRGLIPRSTVCYDQQTNNDFESLWFRLDNLSFSPN-NKFTLVGCNAWALLS-TFGI 148

Query: 153 ESYISGCMALCGNSTETIRDGSCSGSGCCQLE--IPRGLKNLDVEVKSFDNHSKVLNFNP 212
           ++Y +GCM+LC   T    +  C+G GCC+ E  IP     ++ +   F+N + V +FNP
Sbjct: 149 QNYSTGCMSLC--DTPPPPNSKCNGVGCCRTEVSIPLDSHRIETQPSRFENMTSVEHFNP 208

Query: 213 CGYAFVIQQDKFNFST----NYIRNFTQKEVPLVLDWAIPTNKSCLEQAKNKSNCSICGL 272
           C YAF ++   FNFS+      +RN T+   P++LDW+I  N++C EQ   +   +ICG 
Sbjct: 209 CSYAFFVEDGMFNFSSLEDLKDLRNVTR--FPVLLDWSI-GNQTC-EQVVGR---NICGG 268

Query: 273 NTKRINFLDDGSEYRCQCLEGFEGNPYLPQGCQDIDECKNGSQKCKYKELCVNTPGNYTC 332
           N+   +    G  Y C+CL+GF+GNPYL  GCQDI+EC      C     C NT G++ C
Sbjct: 269 NSTCFD-STRGKGYNCKCLQGFDGNPYLSDGCQDINECTTRIHNCSDTSTCENTLGSFHC 328

Query: 333 HCPKNH-------------KGDGRYGGEGCIPNSISSIHIIIVFVFGSIWLYLGYKKWKF 392
            CP                K + +Y G   +    +   +II+     I   + ++  K 
Sbjct: 329 QCPSGSDLNTTTMSCIDTPKEEPKYLGWTTVLLGTTIGFLIILLTISYIQQKMRHR--KN 388

Query: 393 IQQKEKFFKKNGGLMLRQHLSQWQSPDM-LKIFTQEELEKATNKYDEKAVVGKGGYGTVY 452
            + +++FF++NGG ML Q LS     ++ +KIFT+E +++AT+ Y+E  ++G+GG GTVY
Sbjct: 389 TELRQQFFEQNGGGMLIQRLSGAGPSNVDVKIFTEEGMKEATDGYNESRILGQGGQGTVY 448

Query: 453 KGVLDNGLTVAIKKSKLVNQSQIDQFINEVNVLSQINHRNVVKLIWCCLETKVPLLVYEL 512
           KG+L +   VAIKK++L ++SQ++QFINEV VLSQINHRNVVKL+ CCLET+VPLLVYE 
Sbjct: 449 KGILQDNSIVAIKKARLGDRSQVEQFINEVLVLSQINHRNVVKLLGCCLETEVPLLVYEF 508

Query: 513 VTNGTLFEHIHRKTNQVYLPWEARLKIASEIASVVSYLHSSASTPIIHRDIKSSNILLDQ 572
           +++GTLF+H+H       L WE RL+IA E+A  ++YLHS AS PIIHRD+K++NILLD+
Sbjct: 509 ISSGTLFDHLHGSMFDSSLTWEHRLRIAIEVAGTLAYLHSYASIPIIHRDVKTANILLDE 568

Query: 573 NYTAKISDFGTSKLVPLDRTQISTMVQGTIGYLDPEYFLT 587
           N TAK++DFG S+L+P+D+ Q++TMVQGT+GYLDPEY+ T
Sbjct: 569 NLTAKVADFGASRLIPMDQEQLTTMVQGTLGYLDPEYYNT 583

BLAST of Lsi08G004970 vs. ExPASy Swiss-Prot
Match: Q9LMN6 (Wall-associated receptor kinase 4 OS=Arabidopsis thaliana OX=3702 GN=WAK4 PE=2 SV=1)

HSP 1 Score: 400.2 bits (1027), Expect = 4.2e-110
Identity = 236/602 (39.20%), Postives = 349/602 (57.97%), Query Frame = 0

Query: 7   KLIKLIILSSATLFVVAAAVKASFTGCNEQCGNLKIPYPFGIKEGCYHNENFSITCNKTR 66
           K+ +L +++   L  +      +   C E+CGN+ + YPFG   GC+  E+ S   +   
Sbjct: 2   KVQRLFLVAIFCLSYMQLVKGQTLPRCPEKCGNVTLEYPFGFSPGCWRAEDPSFNLSCVN 61

Query: 67  YNPPKAFLKDTDIGITNISIIEGQLPILQSIAKDCYTKNGT-PESNDPTFQLFNFTISNT 126
            N    F K  ++   + S    QL +L   +  CY   G   +       L N T+S  
Sbjct: 62  EN---LFYKGLEVVEISHS---SQLRVLYPASYICYNSKGKFAKGTYYWSNLGNLTLSGN 121

Query: 127 MNKFTVIGCDTYAFISGELEGESYISGCMALCGNSTETIRDGSCSGSGCCQLEIPRGLKN 186
            N  T +GC++YAF+S      + + GC++ C ++     +G C+G GCCQ  +P G   
Sbjct: 122 -NTITALGCNSYAFVSSNGTRRNSV-GCISAC-DALSHEANGECNGEGCCQNPVPAGNNW 181

Query: 187 LDVEVKSFDNHSKV--LNFNPCGYAFVIQQDKFNFSTNYIRNFTQKE---VPLVLDWAIP 246
           L V    FDN + V  ++   C YAF+++  KF ++ +   ++ Q      P+VLDW+I 
Sbjct: 182 LIVRSYRFDNDTSVQPISEGQCIYAFLVENGKFKYNASDKYSYLQNRNVGFPVVLDWSI- 241

Query: 247 TNKSCLEQAKNKSNCSICGLNTKRINFLDDGSEYRCQCLEGFEGNPYLPQGCQDIDECKN 306
             ++C +  + K     CG+N    N    G  Y C+C  GF+GNPYL  GCQDI+EC  
Sbjct: 242 RGETCGQVGEKK-----CGVNGICSNSA-SGIGYTCKCKGGFQGNPYLQNGCQDINECTT 301

Query: 307 GS----QKCKYKELCVNTPGNYTCHCPKNH-----------KGDGRYGGEGCIPNSISSI 366
            +      C     C N  G++ C+C   +           KG+  Y     I    +  
Sbjct: 302 ANPIHKHNCSGDSTCENKLGHFRCNCRSRYELNTTTNTCKPKGNPEYVEWTTIVLGTTIG 361

Query: 367 HIIIVFVFGSIWLYLGYKKWKFIQQKEKFFKKNGGLMLRQHLSQWQSPDM-LKIFTQEEL 426
            ++I+     I   +  K  K  + +++FF++NGG ML Q LS     ++ +KIFT+E +
Sbjct: 362 FLVILLAISCIEHKM--KNTKDTELRQQFFEQNGGGMLMQRLSGAGPSNVDVKIFTEEGM 421

Query: 427 EKATNKYDEKAVVGKGGYGTVYKGVLDNGLTVAIKKSKLVNQSQIDQFINEVNVLSQINH 486
           ++AT+ YDE  ++G+GG GTVYKG+L +   VAIKK++L + SQ++QFINEV VLSQINH
Sbjct: 422 KEATDGYDENRILGQGGQGTVYKGILPDNSIVAIKKARLGDNSQVEQFINEVLVLSQINH 481

Query: 487 RNVVKLIWCCLETKVPLLVYELVTNGTLFEHIHRKTNQVYLPWEARLKIASEIASVVSYL 546
           RNVVKL+ CCLET+VPLLVYE +++GTLF+H+H       L WE RL++A EIA  ++YL
Sbjct: 482 RNVVKLLGCCLETEVPLLVYEFISSGTLFDHLHGSMFDSSLTWEHRLRMAVEIAGTLAYL 541

Query: 547 HSSASTPIIHRDIKSSNILLDQNYTAKISDFGTSKLVPLDRTQISTMVQGTIGYLDPEYF 587
           HSSAS PIIHRDIK++NILLD+N TAK++DFG S+L+P+D+  ++TMVQGT+GYLDPEY+
Sbjct: 542 HSSASIPIIHRDIKTANILLDENLTAKVADFGASRLIPMDKEDLATMVQGTLGYLDPEYY 585

BLAST of Lsi08G004970 vs. ExPASy TrEMBL
Match: A0A5D3DKI3 (Wall-associated receptor kinase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1607G001160 PE=4 SV=1)

HSP 1 Score: 956.8 bits (2472), Expect = 4.3e-275
Identity = 470/597 (78.73%), Postives = 517/597 (86.60%), Query Frame = 0

Query: 1   MRSLEEKLIKLIILSSATLFVVAAAVKASFTGCNEQCGNLKIPYPFGIKEGCYHNENFSI 60
           M+   E L+KLIIL S TL   A  V    TGC+E CGNL+IPYPFGIK+ CY ++ FSI
Sbjct: 1   MKIQAEDLMKLIILLSGTL-AAAVEVAKVLTGCDEHCGNLRIPYPFGIKKRCYLDQAFSI 60

Query: 61  TCNKTRYNPPKAFLKDTDIGITNISIIEGQLPILQSIAKDCYTKNGTPESNDPTFQLFN- 120
           TCNKT Y+PPK FL+D++I ITNISII+GQL I Q +A+DCYTKNG  ESN   F L N 
Sbjct: 61  TCNKT-YHPPKPFLQDSNIEITNISIIQGQLHIKQFVARDCYTKNGPSESNTRPFLLLNK 120

Query: 121 FTISNTMNKFTVIGCDTYAFISGELEGESYISGCMALCG-NSTETIRDGSCSGSGCCQLE 180
           F ISNT NKF VIGCDTYA+I GE+EGESY SGCMALCG N+T+ I+DGSCSG+GCCQL+
Sbjct: 121 FRISNTDNKFIVIGCDTYAYIYGEIEGESYKSGCMALCGNNNTKIIKDGSCSGNGCCQLQ 180

Query: 181 IPRGLKNLDVEVKSFDNHSKVLNFNPCGYAFVIQQDKFNFSTNYIRNFTQKEVPLVLDWA 240
           IP+GLK L++EV+SFDNHS+VL FNPCGYAFVIQQDKF FS  YI NFTQ+EVPLVLDW 
Sbjct: 181 IPKGLKILELEVRSFDNHSEVLKFNPCGYAFVIQQDKFTFSKKYIYNFTQEEVPLVLDWG 240

Query: 241 IPTNKSCLEQAKNKSNCSICGLNTKRINFLDDGSEYRCQCLEGFEGNPYLPQGCQDIDEC 300
           IPTN SC  + +NK NCSICGLN +RI FLDDGSEYRCQCLEGFEGNPYLPQGCQDIDEC
Sbjct: 241 IPTNTSC-SKGENKGNCSICGLNAERIRFLDDGSEYRCQCLEGFEGNPYLPQGCQDIDEC 300

Query: 301 KNGSQKCKYKELCVNTPGNYTCHCPKNHKGDGRYGGEGCIPNSISSIHIII-------VF 360
           KNGS KCKYK+LC NTPGNYTCHCPKNHKGDG+YGGEGC PN +SSIHIII       V 
Sbjct: 301 KNGSHKCKYKDLCFNTPGNYTCHCPKNHKGDGKYGGEGCTPNFMSSIHIIIGTSVGLAVL 360

Query: 361 VFGSIWLYLGYKKWKFIQQKEKFFKKNGGLMLRQHLSQWQSPDMLKIFTQEELEKATNKY 420
           V G IWLYLGY++WKFIQQKEKFFK+NGGLML+QHLSQWQSPDMLKIFT+EELEKATNKY
Sbjct: 361 VIGGIWLYLGYRRWKFIQQKEKFFKRNGGLMLQQHLSQWQSPDMLKIFTREELEKATNKY 420

Query: 421 DEKAVVGKGGYGTVYKGVLDNGLTVAIKKSKLVNQSQIDQFINEVNVLSQINHRNVVKLI 480
           DE AVVGKGGYGTVYKGVLD+G TVAIKKSKLVNQSQ +QFINEV +LSQINHRNVVKLI
Sbjct: 421 DESAVVGKGGYGTVYKGVLDDGSTVAIKKSKLVNQSQTNQFINEVIILSQINHRNVVKLI 480

Query: 481 WCCLETKVPLLVYELVTNGTLFEHIHRKTNQVYLPWEARLKIASEIASVVSYLHSSASTP 540
            CCLETKVPLLVYE +TNGTL EHIH KTNQV LPW  RLKIASEIASV+SYLH SASTP
Sbjct: 481 GCCLETKVPLLVYEFITNGTLSEHIHSKTNQVRLPWATRLKIASEIASVLSYLHYSASTP 540

Query: 541 IIHRDIKSSNILLDQNYTAKISDFGTSKLVPLDRTQISTMVQGTIGYLDPEYFLTND 589
           IIHRDIKS+NILLDQNYTAK+SDFGTSKLVPLDRTQISTMVQGTIGYLDPEYFLT++
Sbjct: 541 IIHRDIKSNNILLDQNYTAKVSDFGTSKLVPLDRTQISTMVQGTIGYLDPEYFLTSE 594

BLAST of Lsi08G004970 vs. ExPASy TrEMBL
Match: A0A1S3B3R7 (wall-associated receptor kinase 2-like OS=Cucumis melo OX=3656 GN=LOC103485671 PE=4 SV=1)

HSP 1 Score: 956.8 bits (2472), Expect = 4.3e-275
Identity = 470/597 (78.73%), Postives = 517/597 (86.60%), Query Frame = 0

Query: 1   MRSLEEKLIKLIILSSATLFVVAAAVKASFTGCNEQCGNLKIPYPFGIKEGCYHNENFSI 60
           M+   E L+KLIIL S TL   A  V    TGC+E CGNL+IPYPFGIK+ CY ++ FSI
Sbjct: 1   MKIQAEDLMKLIILLSGTL-AAAVEVAKVLTGCDEHCGNLRIPYPFGIKKRCYLDQAFSI 60

Query: 61  TCNKTRYNPPKAFLKDTDIGITNISIIEGQLPILQSIAKDCYTKNGTPESNDPTFQLFN- 120
           TCNKT Y+PPK FL+D++I ITNISII+GQL I Q +A+DCYTKNG  ESN   F L N 
Sbjct: 61  TCNKT-YHPPKPFLQDSNIEITNISIIQGQLHIKQFVARDCYTKNGPSESNTRPFLLLNK 120

Query: 121 FTISNTMNKFTVIGCDTYAFISGELEGESYISGCMALCG-NSTETIRDGSCSGSGCCQLE 180
           F ISNT NKF VIGCDTYA+I GE+EGESY SGCMALCG N+T+ I+DGSCSG+GCCQL+
Sbjct: 121 FRISNTDNKFIVIGCDTYAYIYGEIEGESYKSGCMALCGNNNTKIIKDGSCSGNGCCQLQ 180

Query: 181 IPRGLKNLDVEVKSFDNHSKVLNFNPCGYAFVIQQDKFNFSTNYIRNFTQKEVPLVLDWA 240
           IP+GLK L++EV+SFDNHS+VL FNPCGYAFVIQQDKF FS  YI NFTQ+EVPLVLDW 
Sbjct: 181 IPKGLKILELEVRSFDNHSEVLKFNPCGYAFVIQQDKFTFSKKYIYNFTQEEVPLVLDWG 240

Query: 241 IPTNKSCLEQAKNKSNCSICGLNTKRINFLDDGSEYRCQCLEGFEGNPYLPQGCQDIDEC 300
           IPTN SC  + +NK NCSICGLN +RI FLDDGSEYRCQCLEGFEGNPYLPQGCQDIDEC
Sbjct: 241 IPTNTSC-SKGENKGNCSICGLNAERIRFLDDGSEYRCQCLEGFEGNPYLPQGCQDIDEC 300

Query: 301 KNGSQKCKYKELCVNTPGNYTCHCPKNHKGDGRYGGEGCIPNSISSIHIII-------VF 360
           KNGS KCKYK+LC NTPGNYTCHCPKNHKGDG+YGGEGC PN +SSIHIII       V 
Sbjct: 301 KNGSHKCKYKDLCFNTPGNYTCHCPKNHKGDGKYGGEGCTPNFMSSIHIIIGTSVGLAVL 360

Query: 361 VFGSIWLYLGYKKWKFIQQKEKFFKKNGGLMLRQHLSQWQSPDMLKIFTQEELEKATNKY 420
           V G IWLYLGY++WKFIQQKEKFFK+NGGLML+QHLSQWQSPDMLKIFT+EELEKATNKY
Sbjct: 361 VIGGIWLYLGYRRWKFIQQKEKFFKRNGGLMLQQHLSQWQSPDMLKIFTREELEKATNKY 420

Query: 421 DEKAVVGKGGYGTVYKGVLDNGLTVAIKKSKLVNQSQIDQFINEVNVLSQINHRNVVKLI 480
           DE AVVGKGGYGTVYKGVLD+G TVAIKKSKLVNQSQ +QFINEV +LSQINHRNVVKLI
Sbjct: 421 DESAVVGKGGYGTVYKGVLDDGSTVAIKKSKLVNQSQTNQFINEVIILSQINHRNVVKLI 480

Query: 481 WCCLETKVPLLVYELVTNGTLFEHIHRKTNQVYLPWEARLKIASEIASVVSYLHSSASTP 540
            CCLETKVPLLVYE +TNGTL EHIH KTNQV LPW  RLKIASEIASV+SYLH SASTP
Sbjct: 481 GCCLETKVPLLVYEFITNGTLSEHIHSKTNQVRLPWATRLKIASEIASVLSYLHYSASTP 540

Query: 541 IIHRDIKSSNILLDQNYTAKISDFGTSKLVPLDRTQISTMVQGTIGYLDPEYFLTND 589
           IIHRDIKS+NILLDQNYTAK+SDFGTSKLVPLDRTQISTMVQGTIGYLDPEYFLT++
Sbjct: 541 IIHRDIKSNNILLDQNYTAKVSDFGTSKLVPLDRTQISTMVQGTIGYLDPEYFLTSE 594

BLAST of Lsi08G004970 vs. ExPASy TrEMBL
Match: A0A6J1CJM0 (putative wall-associated receptor kinase-like 16 OS=Momordica charantia OX=3673 GN=LOC111011910 PE=4 SV=1)

HSP 1 Score: 776.5 bits (2004), Expect = 7.9e-221
Identity = 386/599 (64.44%), Postives = 474/599 (79.13%), Query Frame = 0

Query: 6   EKLIKLII------LSSATLFVVAAAVKASFTGCNEQCGNLKIPYPFGIKEGCYHNENFS 65
           E L++LII      + SA     AA    +  GC E CG+++IPYPFG+KEGCY NE F 
Sbjct: 2   ETLMRLIITAVQIGILSAAAATAAAGASQALPGCEEWCGDVQIPYPFGMKEGCYLNEKFF 61

Query: 66  ITCNKTRYNPPKAFLKDTDIGITNISIIEGQLPILQSIAKDCYTKNGTPESNDPTFQLFN 125
           I+CNKT  + PKAFL+  ++ +TNIS I G+L IL   A+DCY  N  P+ +   F    
Sbjct: 62  ISCNKTHNDSPKAFLQGGNLNVTNIS-ISGELNILHFWARDCYPPNTLPDLS-TAFLNTE 121

Query: 126 FTISNTMNKFTVIGCDTYAFISGELEG-ESYISGCMALCGNSTETIRDGSCSGSGCCQLE 185
           F +S+  NKFTVIGCDT++FISG + G + Y S C+ALC + T T++DG+CSG+GCCQLE
Sbjct: 122 FAVSSAKNKFTVIGCDTFSFISGLVGGTQFYKSACVALCDDIT-TVKDGACSGNGCCQLE 181

Query: 186 IPRGLKNLDVEVKSFDNHSKVLNFNPCGYAFVIQQDKFNFSTNYIRNFTQKEVPLVLDWA 245
           IP+GL NL+  V SFDNH+ VL+FNPCGYAFVI++DKFNFS+ YIR+F  + VPLVLDWA
Sbjct: 182 IPKGLNNLNYTVSSFDNHTNVLSFNPCGYAFVIEEDKFNFSSKYIRHFPLERVPLVLDWA 241

Query: 246 IPTNKSCLEQAKNKSNCSICGLNTKRINFLDDGSEYRCQCLEGFEGNPYLPQGCQDIDEC 305
           I +N +C    +N +NC ICG +++++NF++DGSEYRCQC +GFEGNPYLP+GCQD+DEC
Sbjct: 242 I-SNNTC----ENTTNC-ICGPSSRKVNFVEDGSEYRCQCSDGFEGNPYLPRGCQDVDEC 301

Query: 306 KNGSQKCKYKELCVNTPGNYTCHCPKNHKGDGRYGGEGCIPNSISSIHIII-------VF 365
           K+G   CK++  CVNT GNYTC+CP+  +GDGR  GEGC  NS S + II+       V 
Sbjct: 302 KDGRHDCKFE--CVNTEGNYTCNCPEGFRGDGRREGEGCTRNSKSFVQIIVGVTVGFTVL 361

Query: 366 VFGSIWLYLGYKKWKFIQQKEKFFKKNGGLMLRQHLSQWQ-SPDMLKIFTQEELEKATNK 425
           + G  W YLGY+KWKF++ KE+FF+KNGGLML+QHLSQWQ S DM++IFTQEELEKATNK
Sbjct: 362 LIGLTWFYLGYRKWKFVKLKEEFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKATNK 421

Query: 426 YDEKAVVGKGGYGTVYKGVLDNGLTVAIKKSKLVNQSQIDQFINEVNVLSQINHRNVVKL 485
           YDE AVVGKGGYGTVYKGVL++GL VAIKKSKLV+QSQ  QFINEV VLSQINHRNVVKL
Sbjct: 422 YDESAVVGKGGYGTVYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKL 481

Query: 486 IWCCLETKVPLLVYELVTNGTLFEHIHRKTNQVY-LPWEARLKIASEIASVVSYLHSSAS 545
           + CCLET+VPLLVYE +TNGTL++HIH K N  Y LPWEARL+IASE A V+SYLHSSAS
Sbjct: 482 LGCCLETQVPLLVYEFITNGTLYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSSAS 541

Query: 546 TPIIHRDIKSSNILLDQNYTAKISDFGTSKLVPLDRTQISTMVQGTIGYLDPEYFLTND 589
           TPIIHRDIK++NILLDQNYTAK+SDFG SKLVPLD+TQ+STMVQGT+GYLDPEY LT++
Sbjct: 542 TPIIHRDIKTTNILLDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSE 589

BLAST of Lsi08G004970 vs. ExPASy TrEMBL
Match: A0A6J1H843 (wall-associated receptor kinase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111461403 PE=4 SV=1)

HSP 1 Score: 775.4 bits (2001), Expect = 1.8e-220
Identity = 387/596 (64.93%), Postives = 474/596 (79.53%), Query Frame = 0

Query: 6    EKLIKLIILSSATLFVVAAAVKAS--FTGCNEQCGNLKIPYPFGIKEGCYHNENFSITCN 65
            E LI+L+I++   + ++++ V AS    GC ++CG+L+IPYPFG +EGCY N+NF ITCN
Sbjct: 713  ETLIRLMIVN---ILILSSPVYASQALLGCPDRCGDLQIPYPFGTREGCYLNKNFLITCN 772

Query: 66   KTRYNPPKAFLKDTDIGITNISIIEGQLPILQSIAKDCYTKNGTPES--NDPTFQLFNFT 125
             T +NPP+ FL+  +I +TNIS I G+L IL   AKDCY KN + ++     T  L  FT
Sbjct: 773  TTHFNPPRPFLRTGNIQVTNIS-ISGELQILHFGAKDCYPKNNSLDARRTSATLNLSTFT 832

Query: 126  ISNTMNKFTVIGCDTYAFISGELEGESYISGCMALCGNSTETIRDGSCSGSGCCQLEIPR 185
            +S+T NKFTVIGCDTYAF+SG++EG+SY + C+ALC N T T+RDG+CSG+GCCQL+IP 
Sbjct: 833  VSSTKNKFTVIGCDTYAFLSGQIEGQSYRTACVALCDNIT-TVRDGACSGNGCCQLDIPS 892

Query: 186  GLKNLDVEVKSFDNHSKVLNFNPCGYAFVIQQDKFNFSTNYIRNFTQKEVPLVLDWAIPT 245
            GLK L   V+SFDNH+ VL+FNPCGYAFV ++DKF+FS  YIR+F Q++VP+VLDW I +
Sbjct: 893  GLKGLRYRVRSFDNHTDVLSFNPCGYAFVTEEDKFHFSAAYIRDFPQRQVPVVLDWGI-S 952

Query: 246  NKSCLEQAKNKSNCSICGLNTKRINFLDDGSEYRCQCLEGFEGNPYLPQGCQDIDECKNG 305
            N +C   A NKSNC ICG N+  +N L DGSEYRC+CL+GFEGNPYLP+GCQDIDEC++ 
Sbjct: 953  NTTC-STANNKSNC-ICGPNSMMVNPLHDGSEYRCRCLDGFEGNPYLPRGCQDIDECRDE 1012

Query: 306  S-QKCKYKELCVNTPGNYTCHCPKNHKGDGRYGGEGCIPNSISSIHIII-------VFVF 365
                CK++  CVNT GNYTC+CP+  KGDGR GGEGC  +S S + +II       V V 
Sbjct: 1013 RLNDCKFE--CVNTKGNYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVIIGVSVGFTVLVI 1072

Query: 366  GSIWLYLGYKKWKFIQQKEKFFKKNGGLMLRQHLSQWQ-SPDMLKIFTQEELEKATNKYD 425
            GS WLYLGY+KWK I+ KEKFF++NGGLML++HLSQW+ S D + IFTQEEL+KATNKYD
Sbjct: 1073 GSTWLYLGYRKWKLIKLKEKFFEENGGLMLQRHLSQWKSSTDTVTIFTQEELDKATNKYD 1132

Query: 426  EKAVVGKGGYGTVYKGVLDNGLTVAIKKSKLVNQSQIDQFINEVNVLSQINHRNVVKLIW 485
            E AV+GKGGYGTVYKG L +G  VAIKKSKLV+QSQ  QFINEV VLSQINHRNVVKL+ 
Sbjct: 1133 ESAVIGKGGYGTVYKGFLPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNVVKLLG 1192

Query: 486  CCLETKVPLLVYELVTNGTLFEHIHRKTNQVYLPWEARLKIASEIASVVSYLHSSASTPI 545
            CCLET+VPLLVYE VTNGTLF+HIH  T  V L WEARL+IASE A V+SYLHSSASTPI
Sbjct: 1193 CCLETQVPLLVYEFVTNGTLFDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSASTPI 1252

Query: 546  IHRDIKSSNILLDQNYTAKISDFGTSKLVPLDRTQISTMVQGTIGYLDPEYFLTND 589
            IHRDIK++NILLD NY AK+SDFG SKLVPLD+TQ+STMVQGT+GYLDPEY LT++
Sbjct: 1253 IHRDIKTTNILLDDNYNAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSE 1298

BLAST of Lsi08G004970 vs. ExPASy TrEMBL
Match: A0A6J1JNA5 (wall-associated receptor kinase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111486858 PE=4 SV=1)

HSP 1 Score: 771.9 bits (1992), Expect = 1.9e-219
Identity = 382/592 (64.53%), Postives = 467/592 (78.89%), Query Frame = 0

Query: 8   LIKLIILSSATLFVVAAAVKASFTGCNEQCGNLKIPYPFGIKEGCYHNENFSITCNKTRY 67
           +I ++ILS +    V A    +  GC+++CG+L+IPYPFG +EGCY N+NF ITCN T +
Sbjct: 13  IINILILSPS----VYAVASQALLGCDDRCGDLQIPYPFGTREGCYLNKNFLITCNTTHF 72

Query: 68  NPPKAFLKDTDIGITNISIIEGQLPILQSIAKDCYTKNGTPES--NDPTFQLFNFTISNT 127
           +PP+ FL+  +I +TNIS I G+L IL   AKDCY KN + ++     T  L  FT+S+T
Sbjct: 73  DPPRPFLRTGNIQVTNIS-ISGELQILHFGAKDCYPKNNSLDARRTSATLNLSTFTVSST 132

Query: 128 MNKFTVIGCDTYAFISGELEGESYISGCMALCGNSTETIRDGSCSGSGCCQLEIPRGLKN 187
            NKFTVIGCDTYAF+SG++EG+SY + C+ALC N T T+RDG+CSG+GCCQL+IP GLK 
Sbjct: 133 KNKFTVIGCDTYAFLSGQIEGQSYRTACVALCDNIT-TVRDGACSGNGCCQLDIPSGLKG 192

Query: 188 LDVEVKSFDNHSKVLNFNPCGYAFVIQQDKFNFSTNYIRNFTQKEVPLVLDWAIPTNKSC 247
           L   V+SFDNH+ V +FNPCGYAFV ++DKF+FS  YIRNF Q++VP+VLDW I +N +C
Sbjct: 193 LRYRVRSFDNHTDVFSFNPCGYAFVTEEDKFHFSAAYIRNFPQRQVPVVLDWGI-SNTTC 252

Query: 248 LEQAKNKSNCSICGLNTKRINFLDDGSEYRCQCLEGFEGNPYLPQGCQDIDECKNGS-QK 307
              A NKSNC ICG N+  +N L DGSEYRC CL+GFEGNPYLP+GCQDIDEC++     
Sbjct: 253 -STANNKSNC-ICGPNSMMVNVLPDGSEYRCGCLDGFEGNPYLPRGCQDIDECRDERLND 312

Query: 308 CKYKELCVNTPGNYTCHCPKNHKGDGRYGGEGCIPNSISSIHIII-------VFVFGSIW 367
           CK++  CVNT GNYTC+CP+  KGDGR GGEGC  +S S + +II       V V GS W
Sbjct: 313 CKFE--CVNTKGNYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVIIGVSVGFTVLVIGSTW 372

Query: 368 LYLGYKKWKFIQQKEKFFKKNGGLMLRQHLSQWQ-SPDMLKIFTQEELEKATNKYDEKAV 427
           LYLGY+KWK I+ KEKFF++NGGLML++HLSQW+ S D + IFTQEEL+KATNKYDE AV
Sbjct: 373 LYLGYRKWKLIKLKEKFFEENGGLMLQRHLSQWKSSTDTVTIFTQEELDKATNKYDESAV 432

Query: 428 VGKGGYGTVYKGVLDNGLTVAIKKSKLVNQSQIDQFINEVNVLSQINHRNVVKLIWCCLE 487
           +GKGGYGTVYKG+L +G  VAIKKSKLV+QSQ  QFINEV VLSQINHRNVVKL+ CCLE
Sbjct: 433 IGKGGYGTVYKGILPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNVVKLLGCCLE 492

Query: 488 TKVPLLVYELVTNGTLFEHIHRKTNQVYLPWEARLKIASEIASVVSYLHSSASTPIIHRD 547
           T+VPLLVYE VTNGTLF+HIH  T  V L W+ARL+IA E A V+SYLHSSASTPIIHRD
Sbjct: 493 TQVPLLVYEFVTNGTLFDHIHDTTKHVPLSWKARLRIALETAGVISYLHSSASTPIIHRD 552

Query: 548 IKSSNILLDQNYTAKISDFGTSKLVPLDRTQISTMVQGTIGYLDPEYFLTND 589
           IK++NILLD NY AK+SDFG SKLVPLD+TQ+STMVQGT+GYLDPEY LT++
Sbjct: 553 IKTTNILLDDNYNAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSE 593

BLAST of Lsi08G004970 vs. NCBI nr
Match: XP_038885912.1 (wall-associated receptor kinase 2-like [Benincasa hispida])

HSP 1 Score: 974.2 bits (2517), Expect = 5.3e-280
Identity = 470/595 (78.99%), Postives = 526/595 (88.40%), Query Frame = 0

Query: 1   MRSLEEKLIKLIILSSATLFVVAAAVKASFTGCNEQCGNLKIPYPFGIKEGCYHNENFSI 60
           MRS  E LIKLIIL SATL   A       TGC+E+CG+LKIPYPFGIK+GCY +E+FSI
Sbjct: 1   MRSATENLIKLIILLSATLAAAAPEAAKGLTGCDERCGDLKIPYPFGIKKGCYLDEDFSI 60

Query: 61  TCNKTRYNPPKAFLKDTDIGITNISIIEGQLPILQSIAKDCYTKNGTPESNDPTFQLFNF 120
           TCNK+ ++PPKAFLKD++  ITNISII+GQL ILQ +AKDCYTK+G  E+  PT +L++ 
Sbjct: 61  TCNKS-HDPPKAFLKDSNTDITNISIIQGQLHILQFVAKDCYTKDGPSEAYTPTLELYDL 120

Query: 121 TISNTMNKFTVIGCDTYAFISGELEGESYISGCMALCGNSTETIRDGSCSGSGCCQLEIP 180
           TISNT NKF VIGCDTYAFISGE+EG+SY SGCMALCGNST+TI+DGSCSG+GCCQLEIP
Sbjct: 121 TISNTDNKFIVIGCDTYAFISGEIEGQSYKSGCMALCGNSTKTIKDGSCSGNGCCQLEIP 180

Query: 181 RGLKNLDVEVKSFDNHSKVLNFNPCGYAFVIQQDKFNFSTNYIRNFTQKEVPLVLDWAIP 240
           +GLK L+++V+SF+NHS+VL FN CGYAFV+QQDKF FS  YI NFTQ+EVPLVLDWAIP
Sbjct: 181 KGLKYLELDVRSFNNHSEVLEFNSCGYAFVVQQDKFTFSEKYIHNFTQEEVPLVLDWAIP 240

Query: 241 TNKSCLEQAKNKSNCSICGLNTKRINFLDDGSEYRCQCLEGFEGNPYLPQGCQDIDECKN 300
            N SCL +A NK+NCSICG NTK I+FLDDGS YRCQCLEGFEGNPYLPQGCQDIDECKN
Sbjct: 241 ANTSCL-KAGNKTNCSICGTNTKTISFLDDGSNYRCQCLEGFEGNPYLPQGCQDIDECKN 300

Query: 301 GSQKCKYKELCVNTPGNYTCHCPKNHKGDGRYGGEGCIPNSISSIHIII-------VFVF 360
           GS KC YK+LCVNT GNYTC+CPKNH+GDGRYGGEGC PNS SSIHIII       V V 
Sbjct: 301 GSHKCTYKDLCVNTQGNYTCYCPKNHEGDGRYGGEGCTPNSRSSIHIIIGICVGFAVLVI 360

Query: 361 GSIWLYLGYKKWKFIQQKEKFFKKNGGLMLRQHLSQWQSPDMLKIFTQEELEKATNKYDE 420
           GS+WLYLGYKKWKFIQQKEKFFKKNGGLML++HLS+WQSPDMLKIFTQE+LEKATNKYD+
Sbjct: 361 GSVWLYLGYKKWKFIQQKEKFFKKNGGLMLQKHLSEWQSPDMLKIFTQEKLEKATNKYDD 420

Query: 421 KAVVGKGGYGTVYKGVLDNGLTVAIKKSKLVNQSQIDQFINEVNVLSQINHRNVVKLIWC 480
            A++GKGG+GTVYKGVLD+G TVAIKKSKLVNQSQ +QFINEV VLSQINHRNVVKLI C
Sbjct: 421 SAMIGKGGFGTVYKGVLDDGSTVAIKKSKLVNQSQTNQFINEVIVLSQINHRNVVKLIGC 480

Query: 481 CLETKVPLLVYELVTNGTLFEHIHRKTNQVYLPWEARLKIASEIASVVSYLHSSASTPII 540
           CLETKVPLLVYE V N TLFEHIHR+TNQ YLPWEARLKIASEIASV+SYLHSS STPII
Sbjct: 481 CLETKVPLLVYEFVNNRTLFEHIHRRTNQAYLPWEARLKIASEIASVLSYLHSSTSTPII 540

Query: 541 HRDIKSSNILLDQNYTAKISDFGTSKLVPLDRTQISTMVQGTIGYLDPEYFLTND 589
           HRDIKS+NILLDQNYTAK+SDFG SKLVPLD TQISTMVQGTIGYLDPEY+LT++
Sbjct: 541 HRDIKSNNILLDQNYTAKVSDFGMSKLVPLDGTQISTMVQGTIGYLDPEYYLTSE 593

BLAST of Lsi08G004970 vs. NCBI nr
Match: XP_008441592.1 (PREDICTED: wall-associated receptor kinase 2-like [Cucumis melo] >TYK23789.1 wall-associated receptor kinase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 956.8 bits (2472), Expect = 8.8e-275
Identity = 470/597 (78.73%), Postives = 517/597 (86.60%), Query Frame = 0

Query: 1   MRSLEEKLIKLIILSSATLFVVAAAVKASFTGCNEQCGNLKIPYPFGIKEGCYHNENFSI 60
           M+   E L+KLIIL S TL   A  V    TGC+E CGNL+IPYPFGIK+ CY ++ FSI
Sbjct: 1   MKIQAEDLMKLIILLSGTL-AAAVEVAKVLTGCDEHCGNLRIPYPFGIKKRCYLDQAFSI 60

Query: 61  TCNKTRYNPPKAFLKDTDIGITNISIIEGQLPILQSIAKDCYTKNGTPESNDPTFQLFN- 120
           TCNKT Y+PPK FL+D++I ITNISII+GQL I Q +A+DCYTKNG  ESN   F L N 
Sbjct: 61  TCNKT-YHPPKPFLQDSNIEITNISIIQGQLHIKQFVARDCYTKNGPSESNTRPFLLLNK 120

Query: 121 FTISNTMNKFTVIGCDTYAFISGELEGESYISGCMALCG-NSTETIRDGSCSGSGCCQLE 180
           F ISNT NKF VIGCDTYA+I GE+EGESY SGCMALCG N+T+ I+DGSCSG+GCCQL+
Sbjct: 121 FRISNTDNKFIVIGCDTYAYIYGEIEGESYKSGCMALCGNNNTKIIKDGSCSGNGCCQLQ 180

Query: 181 IPRGLKNLDVEVKSFDNHSKVLNFNPCGYAFVIQQDKFNFSTNYIRNFTQKEVPLVLDWA 240
           IP+GLK L++EV+SFDNHS+VL FNPCGYAFVIQQDKF FS  YI NFTQ+EVPLVLDW 
Sbjct: 181 IPKGLKILELEVRSFDNHSEVLKFNPCGYAFVIQQDKFTFSKKYIYNFTQEEVPLVLDWG 240

Query: 241 IPTNKSCLEQAKNKSNCSICGLNTKRINFLDDGSEYRCQCLEGFEGNPYLPQGCQDIDEC 300
           IPTN SC  + +NK NCSICGLN +RI FLDDGSEYRCQCLEGFEGNPYLPQGCQDIDEC
Sbjct: 241 IPTNTSC-SKGENKGNCSICGLNAERIRFLDDGSEYRCQCLEGFEGNPYLPQGCQDIDEC 300

Query: 301 KNGSQKCKYKELCVNTPGNYTCHCPKNHKGDGRYGGEGCIPNSISSIHIII-------VF 360
           KNGS KCKYK+LC NTPGNYTCHCPKNHKGDG+YGGEGC PN +SSIHIII       V 
Sbjct: 301 KNGSHKCKYKDLCFNTPGNYTCHCPKNHKGDGKYGGEGCTPNFMSSIHIIIGTSVGLAVL 360

Query: 361 VFGSIWLYLGYKKWKFIQQKEKFFKKNGGLMLRQHLSQWQSPDMLKIFTQEELEKATNKY 420
           V G IWLYLGY++WKFIQQKEKFFK+NGGLML+QHLSQWQSPDMLKIFT+EELEKATNKY
Sbjct: 361 VIGGIWLYLGYRRWKFIQQKEKFFKRNGGLMLQQHLSQWQSPDMLKIFTREELEKATNKY 420

Query: 421 DEKAVVGKGGYGTVYKGVLDNGLTVAIKKSKLVNQSQIDQFINEVNVLSQINHRNVVKLI 480
           DE AVVGKGGYGTVYKGVLD+G TVAIKKSKLVNQSQ +QFINEV +LSQINHRNVVKLI
Sbjct: 421 DESAVVGKGGYGTVYKGVLDDGSTVAIKKSKLVNQSQTNQFINEVIILSQINHRNVVKLI 480

Query: 481 WCCLETKVPLLVYELVTNGTLFEHIHRKTNQVYLPWEARLKIASEIASVVSYLHSSASTP 540
            CCLETKVPLLVYE +TNGTL EHIH KTNQV LPW  RLKIASEIASV+SYLH SASTP
Sbjct: 481 GCCLETKVPLLVYEFITNGTLSEHIHSKTNQVRLPWATRLKIASEIASVLSYLHYSASTP 540

Query: 541 IIHRDIKSSNILLDQNYTAKISDFGTSKLVPLDRTQISTMVQGTIGYLDPEYFLTND 589
           IIHRDIKS+NILLDQNYTAK+SDFGTSKLVPLDRTQISTMVQGTIGYLDPEYFLT++
Sbjct: 541 IIHRDIKSNNILLDQNYTAKVSDFGTSKLVPLDRTQISTMVQGTIGYLDPEYFLTSE 594

BLAST of Lsi08G004970 vs. NCBI nr
Match: XP_038886240.1 (wall-associated receptor kinase 2-like [Benincasa hispida])

HSP 1 Score: 811.6 bits (2095), Expect = 4.6e-231
Identity = 394/599 (65.78%), Postives = 479/599 (79.97%), Query Frame = 0

Query: 1   MRSLEEKLIKLIILSSATLFVVAAAVKASFTGCNEQCGNLKIPYPFGIKEGCYHNENFSI 60
           M   EEKL+ L +L       +A A  AS   C  QCGNL+IPYPFG+K+GCY NENF +
Sbjct: 1   MEHPEEKLLFLFMLIMFPSVALATAAAAS-QDCERQCGNLEIPYPFGMKKGCYLNENFLV 60

Query: 61  TCNKTRYNPPKAFLKDTDIGITNISIIEGQLPILQSIAKDCYTKNGTPESN--DPTFQLF 120
           TCNKT Y+PPKAFL+ ++I +T+ISI+  +L IL  +A+DCYTK+G P+ +   P   + 
Sbjct: 61  TCNKTHYHPPKAFLRGSNIEVTHISILPSELHILNYVARDCYTKDGRPDISRARPFLVVD 120

Query: 121 NFTISNTMNKFTVIGCDTYAFISGELEGESYISGCMALCGNSTETIRDGSCSGSGCCQLE 180
            F+ISNT NK T++GCDTY ++ GE++GE Y SGCMALCGNS+ TI+DGSCSGSGCCQLE
Sbjct: 121 MFSISNTKNKLTIVGCDTYGYLHGEIQGEIYSSGCMALCGNSSRTIKDGSCSGSGCCQLE 180

Query: 181 IPRGLKNLDVEVKSFDNHSKVLNFNPCGYAFVIQQDKFNFSTNYIRNFTQKEVPLVLDWA 240
           IP+GLK + ++V+SF+NH++V + NPCGYAFVIQQ++F FS NYI+NFT+ +VPLVLDW 
Sbjct: 181 IPKGLKEIQLDVRSFNNHTRVHSLNPCGYAFVIQQNRFTFSKNYIKNFTESKVPLVLDWG 240

Query: 241 IPTNKSCLEQAKNKSNCSICGLNTKRINFLD-DGSEYRCQCLEGFEGNPYLPQGCQDIDE 300
           I  N +C  Q  N  +  +CG N+++ + L  DGSEY CQCL+GF GNPYL QGCQDIDE
Sbjct: 241 I-KNDTC--QPPNHKDKCLCGPNSQKNSSLSLDGSEYYCQCLDGFHGNPYLSQGCQDIDE 300

Query: 301 CKNGSQKCKYKELCVNTPGNYTCHCPKNHKGDGRYGGEGCIPNSISSIHIIIVFVFG--- 360
           C++GS +CK+K  CVNTPGNYTC+CPKN+KGDGR GGEGC  N+   I III  V G   
Sbjct: 301 CQDGSHECKFKHQCVNTPGNYTCNCPKNYKGDGRRGGEGCTQNTTFFILIIIGIVVGLLV 360

Query: 361 ----SIWLYLGYKKWKFIQQKEKFFKKNGGLMLRQHLSQWQ-SPDMLKIFTQEELEKATN 420
               S+WLYL YKKW+FIQQK KFF KNGGL+L++H+SQWQ S DML+IFT+EELEKATN
Sbjct: 361 LGISSMWLYLAYKKWRFIQQKAKFFNKNGGLVLQEHISQWQSSSDMLRIFTREELEKATN 420

Query: 421 KYDEKAVVGKGGYGTVYKGVLDNGLTVAIKKSKLVNQSQIDQFINEVNVLSQINHRNVVK 480
            +DE  VVGKGGYGTVYKGVL +G  +AIKKSKLV+QSQ  QFINEV +LSQINHRNVVK
Sbjct: 421 NFDESVVVGKGGYGTVYKGVLMDGSIIAIKKSKLVDQSQTKQFINEVIILSQINHRNVVK 480

Query: 481 LIWCCLETKVPLLVYELVTNGTLFEHIHRKTNQVYLPWEARLKIASEIASVVSYLHSSAS 540
           L+ CCLET+VPLLVYE +TNGTLFEHIHRK N  +L W+ RLKIAS+ A V+SYLHSSAS
Sbjct: 481 LLGCCLETRVPLLVYEFITNGTLFEHIHRKNNHSFLSWKTRLKIASQTAGVLSYLHSSAS 540

Query: 541 TPIIHRDIKSSNILLDQNYTAKISDFGTSKLVPLDRTQISTMVQGTIGYLDPEYFLTND 589
           TPIIHRDIKS+NILLD NYTAK+SDFG SKLVPLD+TQISTMVQGT+GYLDPEY LT++
Sbjct: 541 TPIIHRDIKSTNILLDHNYTAKVSDFGASKLVPLDQTQISTMVQGTLGYLDPEYLLTSE 595

BLAST of Lsi08G004970 vs. NCBI nr
Match: XP_022141581.1 (putative wall-associated receptor kinase-like 16 [Momordica charantia])

HSP 1 Score: 776.5 bits (2004), Expect = 1.6e-220
Identity = 386/599 (64.44%), Postives = 474/599 (79.13%), Query Frame = 0

Query: 6   EKLIKLII------LSSATLFVVAAAVKASFTGCNEQCGNLKIPYPFGIKEGCYHNENFS 65
           E L++LII      + SA     AA    +  GC E CG+++IPYPFG+KEGCY NE F 
Sbjct: 2   ETLMRLIITAVQIGILSAAAATAAAGASQALPGCEEWCGDVQIPYPFGMKEGCYLNEKFF 61

Query: 66  ITCNKTRYNPPKAFLKDTDIGITNISIIEGQLPILQSIAKDCYTKNGTPESNDPTFQLFN 125
           I+CNKT  + PKAFL+  ++ +TNIS I G+L IL   A+DCY  N  P+ +   F    
Sbjct: 62  ISCNKTHNDSPKAFLQGGNLNVTNIS-ISGELNILHFWARDCYPPNTLPDLS-TAFLNTE 121

Query: 126 FTISNTMNKFTVIGCDTYAFISGELEG-ESYISGCMALCGNSTETIRDGSCSGSGCCQLE 185
           F +S+  NKFTVIGCDT++FISG + G + Y S C+ALC + T T++DG+CSG+GCCQLE
Sbjct: 122 FAVSSAKNKFTVIGCDTFSFISGLVGGTQFYKSACVALCDDIT-TVKDGACSGNGCCQLE 181

Query: 186 IPRGLKNLDVEVKSFDNHSKVLNFNPCGYAFVIQQDKFNFSTNYIRNFTQKEVPLVLDWA 245
           IP+GL NL+  V SFDNH+ VL+FNPCGYAFVI++DKFNFS+ YIR+F  + VPLVLDWA
Sbjct: 182 IPKGLNNLNYTVSSFDNHTNVLSFNPCGYAFVIEEDKFNFSSKYIRHFPLERVPLVLDWA 241

Query: 246 IPTNKSCLEQAKNKSNCSICGLNTKRINFLDDGSEYRCQCLEGFEGNPYLPQGCQDIDEC 305
           I +N +C    +N +NC ICG +++++NF++DGSEYRCQC +GFEGNPYLP+GCQD+DEC
Sbjct: 242 I-SNNTC----ENTTNC-ICGPSSRKVNFVEDGSEYRCQCSDGFEGNPYLPRGCQDVDEC 301

Query: 306 KNGSQKCKYKELCVNTPGNYTCHCPKNHKGDGRYGGEGCIPNSISSIHIII-------VF 365
           K+G   CK++  CVNT GNYTC+CP+  +GDGR  GEGC  NS S + II+       V 
Sbjct: 302 KDGRHDCKFE--CVNTEGNYTCNCPEGFRGDGRREGEGCTRNSKSFVQIIVGVTVGFTVL 361

Query: 366 VFGSIWLYLGYKKWKFIQQKEKFFKKNGGLMLRQHLSQWQ-SPDMLKIFTQEELEKATNK 425
           + G  W YLGY+KWKF++ KE+FF+KNGGLML+QHLSQWQ S DM++IFTQEELEKATNK
Sbjct: 362 LIGLTWFYLGYRKWKFVKLKEEFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKATNK 421

Query: 426 YDEKAVVGKGGYGTVYKGVLDNGLTVAIKKSKLVNQSQIDQFINEVNVLSQINHRNVVKL 485
           YDE AVVGKGGYGTVYKGVL++GL VAIKKSKLV+QSQ  QFINEV VLSQINHRNVVKL
Sbjct: 422 YDESAVVGKGGYGTVYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKL 481

Query: 486 IWCCLETKVPLLVYELVTNGTLFEHIHRKTNQVY-LPWEARLKIASEIASVVSYLHSSAS 545
           + CCLET+VPLLVYE +TNGTL++HIH K N  Y LPWEARL+IASE A V+SYLHSSAS
Sbjct: 482 LGCCLETQVPLLVYEFITNGTLYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSSAS 541

Query: 546 TPIIHRDIKSSNILLDQNYTAKISDFGTSKLVPLDRTQISTMVQGTIGYLDPEYFLTND 589
           TPIIHRDIK++NILLDQNYTAK+SDFG SKLVPLD+TQ+STMVQGT+GYLDPEY LT++
Sbjct: 542 TPIIHRDIKTTNILLDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSE 589

BLAST of Lsi08G004970 vs. NCBI nr
Match: XP_022960687.1 (wall-associated receptor kinase 3-like [Cucurbita moschata])

HSP 1 Score: 775.4 bits (2001), Expect = 3.6e-220
Identity = 387/596 (64.93%), Postives = 474/596 (79.53%), Query Frame = 0

Query: 6    EKLIKLIILSSATLFVVAAAVKAS--FTGCNEQCGNLKIPYPFGIKEGCYHNENFSITCN 65
            E LI+L+I++   + ++++ V AS    GC ++CG+L+IPYPFG +EGCY N+NF ITCN
Sbjct: 713  ETLIRLMIVN---ILILSSPVYASQALLGCPDRCGDLQIPYPFGTREGCYLNKNFLITCN 772

Query: 66   KTRYNPPKAFLKDTDIGITNISIIEGQLPILQSIAKDCYTKNGTPES--NDPTFQLFNFT 125
             T +NPP+ FL+  +I +TNIS I G+L IL   AKDCY KN + ++     T  L  FT
Sbjct: 773  TTHFNPPRPFLRTGNIQVTNIS-ISGELQILHFGAKDCYPKNNSLDARRTSATLNLSTFT 832

Query: 126  ISNTMNKFTVIGCDTYAFISGELEGESYISGCMALCGNSTETIRDGSCSGSGCCQLEIPR 185
            +S+T NKFTVIGCDTYAF+SG++EG+SY + C+ALC N T T+RDG+CSG+GCCQL+IP 
Sbjct: 833  VSSTKNKFTVIGCDTYAFLSGQIEGQSYRTACVALCDNIT-TVRDGACSGNGCCQLDIPS 892

Query: 186  GLKNLDVEVKSFDNHSKVLNFNPCGYAFVIQQDKFNFSTNYIRNFTQKEVPLVLDWAIPT 245
            GLK L   V+SFDNH+ VL+FNPCGYAFV ++DKF+FS  YIR+F Q++VP+VLDW I +
Sbjct: 893  GLKGLRYRVRSFDNHTDVLSFNPCGYAFVTEEDKFHFSAAYIRDFPQRQVPVVLDWGI-S 952

Query: 246  NKSCLEQAKNKSNCSICGLNTKRINFLDDGSEYRCQCLEGFEGNPYLPQGCQDIDECKNG 305
            N +C   A NKSNC ICG N+  +N L DGSEYRC+CL+GFEGNPYLP+GCQDIDEC++ 
Sbjct: 953  NTTC-STANNKSNC-ICGPNSMMVNPLHDGSEYRCRCLDGFEGNPYLPRGCQDIDECRDE 1012

Query: 306  S-QKCKYKELCVNTPGNYTCHCPKNHKGDGRYGGEGCIPNSISSIHIII-------VFVF 365
                CK++  CVNT GNYTC+CP+  KGDGR GGEGC  +S S + +II       V V 
Sbjct: 1013 RLNDCKFE--CVNTKGNYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVIIGVSVGFTVLVI 1072

Query: 366  GSIWLYLGYKKWKFIQQKEKFFKKNGGLMLRQHLSQWQ-SPDMLKIFTQEELEKATNKYD 425
            GS WLYLGY+KWK I+ KEKFF++NGGLML++HLSQW+ S D + IFTQEEL+KATNKYD
Sbjct: 1073 GSTWLYLGYRKWKLIKLKEKFFEENGGLMLQRHLSQWKSSTDTVTIFTQEELDKATNKYD 1132

Query: 426  EKAVVGKGGYGTVYKGVLDNGLTVAIKKSKLVNQSQIDQFINEVNVLSQINHRNVVKLIW 485
            E AV+GKGGYGTVYKG L +G  VAIKKSKLV+QSQ  QFINEV VLSQINHRNVVKL+ 
Sbjct: 1133 ESAVIGKGGYGTVYKGFLPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNVVKLLG 1192

Query: 486  CCLETKVPLLVYELVTNGTLFEHIHRKTNQVYLPWEARLKIASEIASVVSYLHSSASTPI 545
            CCLET+VPLLVYE VTNGTLF+HIH  T  V L WEARL+IASE A V+SYLHSSASTPI
Sbjct: 1193 CCLETQVPLLVYEFVTNGTLFDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSASTPI 1252

Query: 546  IHRDIKSSNILLDQNYTAKISDFGTSKLVPLDRTQISTMVQGTIGYLDPEYFLTND 589
            IHRDIK++NILLD NY AK+SDFG SKLVPLD+TQ+STMVQGT+GYLDPEY LT++
Sbjct: 1253 IHRDIKTTNILLDDNYNAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSE 1298

BLAST of Lsi08G004970 vs. TAIR 10
Match: AT1G21270.1 (wall-associated kinase 2 )

HSP 1 Score: 472.6 bits (1215), Expect = 4.7e-133
Identity = 259/574 (45.12%), Postives = 370/574 (64.46%), Query Frame = 0

Query: 33  CNEQCGNLKIPYPFGIKEGCYH--NENFSITCNKTRYNPPKAFLKDTDIGITNISIIEGQ 92
           C  +CGN+ + YPFG   GCY+  +E+F++TCN+      K F    ++ + N+S + GQ
Sbjct: 29  CQTRCGNVAVEYPFGTSPGCYYPGDESFNLTCNEQE----KLFF--GNMPVINMS-LSGQ 88

Query: 93  LPILQSIAKDCYTKNGTPESNDPTFQ---LFNFTISNTMNKFTVIGCDTYAFI--SGELE 152
           L +    ++ CY   G  +  D   Q   L NFT+S  +N+FTV+GC++YAF+  SG   
Sbjct: 89  LRVRLVRSRVCYDSQG--KQTDYIAQRTTLGNFTLSE-LNRFTVVGCNSYAFLRTSGV-- 148

Query: 153 GESYISGCMALCGNSTETIRDGSCSGSGCCQLEIPRGLKNLDVEVKSFDNHSKVLNFNPC 212
            E Y +GC+++C ++  T ++GSCSG GCCQ+ +PRG   + V+  SF NH  V  FNPC
Sbjct: 149 -EKYSTGCISICDSA--TTKNGSCSGEGCCQIPVPRGYSFVRVKPHSFHNHPTVHLFNPC 208

Query: 213 GYAFVIQQDKFNF----STNYIRNFTQKEVPLVLDWAIPTNKSCLEQAKNKSNCSICGLN 272
            YAF+++   F+F      N +RN T    P+VLDW+I  +K+C    K      +CG N
Sbjct: 209 TYAFLVEDGMFDFHALEDLNNLRNVT--TFPVVLDWSI-GDKTC----KQVEYRGVCGGN 268

Query: 273 TKRINFLDDGSEYRCQCLEGFEGNPYLPQGCQDIDECKNGSQKCKYKELCVNTPGNYTCH 332
           +   +    G+ Y C+CLEGFEGNPYLP GCQDI+EC +    C     C NT G++ C+
Sbjct: 269 STCFD-STGGTGYNCKCLEGFEGNPYLPNGCQDINECISSRHNCSEHSTCENTKGSFNCN 328

Query: 333 CPKNHKGDGRYGGEGCI-PNSISSIHIIIVFVFGSIWLYLG-------YKKWKFIQQKEK 392
           CP  ++ D        + P       I +    G   + LG        K  K  + ++K
Sbjct: 329 CPSGYRKDSLNSCTRKVRPEYFRWTQIFLGTTIGFSVIMLGISCLQQKIKHRKNTELRQK 388

Query: 393 FFKKNGGLMLRQHLSQWQSPDM-LKIFTQEELEKATNKYDEKAVVGKGGYGTVYKGVLDN 452
           FF++NGG ML Q +S     ++ +KIFT++ +++ATN Y E  ++G+GG GTVYKG+L +
Sbjct: 389 FFEQNGGGMLIQRVSGAGPSNVDVKIFTEKGMKEATNGYHESRILGQGGQGTVYKGILPD 448

Query: 453 GLTVAIKKSKLVNQSQIDQFINEVNVLSQINHRNVVKLIWCCLETKVPLLVYELVTNGTL 512
              VAIKK++L N+SQ++QFINEV VLSQINHRNVVK++ CCLET+VPLLVYE + +GTL
Sbjct: 449 NSIVAIKKARLGNRSQVEQFINEVLVLSQINHRNVVKVLGCCLETEVPLLVYEFINSGTL 508

Query: 513 FEHIHRKTNQVYLPWEARLKIASEIASVVSYLHSSASTPIIHRDIKSSNILLDQNYTAKI 572
           F+H+H       L WE RL+IA+E+A  ++YLHSSAS PIIHRDIK++NILLD+N TAK+
Sbjct: 509 FDHLHGSLYDSSLTWEHRLRIATEVAGSLAYLHSSASIPIIHRDIKTANILLDKNLTAKV 568

Query: 573 SDFGTSKLVPLDRTQISTMVQGTIGYLDPEYFLT 587
           +DFG S+L+P+D+ Q++T+VQGT+GYLDPEY+ T
Sbjct: 569 ADFGASRLIPMDKEQLTTIVQGTLGYLDPEYYNT 579

BLAST of Lsi08G004970 vs. TAIR 10
Match: AT1G21250.1 (cell wall-associated kinase )

HSP 1 Score: 449.1 bits (1154), Expect = 5.6e-126
Identity = 248/574 (43.21%), Postives = 355/574 (61.85%), Query Frame = 0

Query: 33  CNEQCGNLKIPYPFGIKEGCYH--NENFSITCNKTRYNPPKAFLKDTDIGITNISIIEGQ 92
           C  +CGN+ I YPFGI  GCY+  NE+FSITC + R +     L D ++   N S   GQ
Sbjct: 32  CQNKCGNITIEYPFGISSGCYYPGNESFSITCKEDRPH----VLSDIEVANFNHS---GQ 91

Query: 93  LPILQSIAKDCYTKNGTPESNDPTFQLFNFTISNTMNKFTVIGCDTYAFISGELEGESYI 152
           L +L + +  CY + G     D +F L N ++S   NK T +GC+  + +      ++Y 
Sbjct: 92  LQVLLNRSSTCYDEQGKKTEEDSSFTLENLSLS-ANNKLTAVGCNALSLLD-TFGMQNYS 151

Query: 153 SGCMALCGNSTETIRDGSCSGSGCCQLEIPRGLKNLDVEVKS--FDNHSKVLNFNPCGYA 212
           + C++LC +  E   DG C+G GCC++++   L +   E  S    + +   +F+PC YA
Sbjct: 152 TACLSLCDSPPEA--DGECNGRGCCRVDVSAPLDSYTFETTSGRIKHMTSFHDFSPCTYA 211

Query: 213 FVIQQDKFNFSTN----YIRNFTQKEVPLVLDWAIPTNKSCLEQAKNKSNCSICGLNTKR 272
           F+++ DKFNFS+      +RN  +   P++LDW++  N++C    +   + SICG N+  
Sbjct: 212 FLVEDDKFNFSSTEDLLNLRNVMR--FPVLLDWSV-GNQTC----EQVGSTSICGGNSTC 271

Query: 273 INFLDDGSEYRCQCLEGFEGNPYLPQGCQDIDECKNGS----QKCKYKELCVNTPGNYTC 332
           ++       Y C+C EGF+GNPYL  GCQD++EC   S      C   + C N  G + C
Sbjct: 272 LDSTPRNG-YICRCNEGFDGNPYLSAGCQDVNECTTSSTIHRHNCSDPKTCRNKVGGFYC 331

Query: 333 HCPKNHKGDGRYGGEGCIPNSISSIHIIIVFVFGSIWLYLG-------YKKWKFIQQKEK 392
            C   ++ D       C     +   I++V   G + + LG        K  K  + +E+
Sbjct: 332 KCQSGYRLDTT--TMSCKRKEFAWTTILLVTTIGFLVILLGVACIQQRMKHLKDTKLREQ 391

Query: 393 FFKKNGGLMLRQHLSQWQSPDM-LKIFTQEELEKATNKYDEKAVVGKGGYGTVYKGVLDN 452
           FF++NGG ML Q LS     ++ +KIFT++ ++KATN Y E  ++G+GG GTVYKG+L +
Sbjct: 392 FFEQNGGGMLTQRLSGAGPSNVDVKIFTEDGMKKATNGYAESRILGQGGQGTVYKGILPD 451

Query: 453 GLTVAIKKSKLVNQSQIDQFINEVNVLSQINHRNVVKLIWCCLETKVPLLVYELVTNGTL 512
              VAIKK++L + SQ++QFINEV VLSQINHRNVVKL+ CCLET+VPLLVYE +TNGTL
Sbjct: 452 NSIVAIKKARLGDSSQVEQFINEVLVLSQINHRNVVKLLGCCLETEVPLLVYEFITNGTL 511

Query: 513 FEHIHRKTNQVYLPWEARLKIASEIASVVSYLHSSASTPIIHRDIKSSNILLDQNYTAKI 572
           F+H+H       L WE RLKIA E+A  ++YLHSSAS PIIHRDIK++NILLD N TAK+
Sbjct: 512 FDHLHGSMIDSSLTWEHRLKIAIEVAGTLAYLHSSASIPIIHRDIKTANILLDVNLTAKV 571

Query: 573 SDFGTSKLVPLDRTQISTMVQGTIGYLDPEYFLT 587
           +DFG S+L+P+D+ ++ TMVQGT+GYLDPEY+ T
Sbjct: 572 ADFGASRLIPMDKEELETMVQGTLGYLDPEYYNT 584

BLAST of Lsi08G004970 vs. TAIR 10
Match: AT1G21240.1 (wall associated kinase 3 )

HSP 1 Score: 438.3 bits (1126), Expect = 9.8e-123
Identity = 247/580 (42.59%), Postives = 352/580 (60.69%), Query Frame = 0

Query: 33  CNEQCGNLKIPYPFGIKEGCYH--NENFSITCNKTRYNPPKAFLKDTDIGITNISIIEGQ 92
           C  +CGN+ I YPFGI  GCY+  ++NF++TC        +  L    I +TNIS   G 
Sbjct: 31  CKLKCGNVTIEYPFGISTGCYYPGDDNFNLTC-----VVEEKLLLFGIIQVTNIS-HSGH 90

Query: 93  LPILQSIAKDCYTKNGTPESNDPTFQLFNFTISNTMNKFTVIGCDTYAFISGELEGESYI 152
           + +L     +CY +          +QL +    ++ NKFT++GC+  + +S     ++Y 
Sbjct: 91  VSVLFERFSECYEQKNETNGTALGYQLGSSFSLSSNNKFTLVGCNALSLLS-TFGKQNYS 150

Query: 153 SGCMALCGNSTETIRDGSCSGSGCCQLE---IPRGLKNL---DVEVKSFDNHS------K 212
           +GC++LC +  E   +G C+G GCC  E   +P          V +++  N+S       
Sbjct: 151 TGCLSLCNSQPEA--NGRCNGVGCCTTEDFSVPFDSDTFQFGSVRLRNQVNNSLDLFNTS 210

Query: 213 VLNFNPCGYAFVIQQDKFNFSTN----YIRNFTQKEVPLVLDWAIPTNKSCLEQAKNKSN 272
           V  FNPC YAF+++  KFNF ++     +RN T+   P+ LDW+I  N++C EQA    +
Sbjct: 211 VYQFNPCTYAFLVEDGKFNFDSSKDLKNLRNVTR--FPVALDWSI-GNQTC-EQA---GS 270

Query: 273 CSICGLNTKRINFLDDGSEYRCQCLEGFEGNPYLPQGCQDIDECKNGSQKCKYKELCVNT 332
             ICG N+   N       Y C+C EG++GNPY  +GC+DIDEC + +  C   + C N 
Sbjct: 271 TRICGKNSSCYNSTTRNG-YICKCNEGYDGNPYRSEGCKDIDECISDTHNCSDPKTCRNR 330

Query: 333 PGNYTCHCPKNHKGDGRYGGEGCIPNSISSIHIIIVFVFGSIWLYL-------GYKKWKF 392
            G + C CP    G        C         I +V + G + L L         K+ K+
Sbjct: 331 DGGFDCKCP---SGYDLNSSMSCTRPEYKRTRIFLVIIIGVLVLLLAAICIQHATKQRKY 390

Query: 393 IQQKEKFFKKNGGLMLRQHLSQWQSPDM-LKIFTQEELEKATNKYDEKAVVGKGGYGTVY 452
            + + +FF++NGG ML Q LS     ++  KIFT+E +++ATN YDE  ++G+GG GTVY
Sbjct: 391 TKLRRQFFEQNGGGMLIQRLSGAGLSNIDFKIFTEEGMKEATNGYDESRILGQGGQGTVY 450

Query: 453 KGVLDNGLTVAIKKSKLVNQSQIDQFINEVNVLSQINHRNVVKLIWCCLETKVPLLVYEL 512
           KG+L +   VAIKK++L +  Q+DQFI+EV VLSQINHRNVVK++ CCLET+VPLLVYE 
Sbjct: 451 KGILPDNTIVAIKKARLADSRQVDQFIHEVLVLSQINHRNVVKILGCCLETEVPLLVYEF 510

Query: 513 VTNGTLFEHIHRKTNQVYLPWEARLKIASEIASVVSYLHSSASTPIIHRDIKSSNILLDQ 572
           +TNGTLF+H+H       L WE RL+IA E+A  ++YLHSSAS PIIHRDIK++NILLD+
Sbjct: 511 ITNGTLFDHLHGSIFDSSLTWEHRLRIAIEVAGTLAYLHSSASIPIIHRDIKTANILLDE 570

Query: 573 NYTAKISDFGTSKLVPLDRTQISTMVQGTIGYLDPEYFLT 587
           N TAK++DFG SKL+P+D+ Q++TMVQGT+GYLDPEY+ T
Sbjct: 571 NLTAKVADFGASKLIPMDKEQLTTMVQGTLGYLDPEYYTT 590

BLAST of Lsi08G004970 vs. TAIR 10
Match: AT1G21230.1 (wall associated kinase 5 )

HSP 1 Score: 435.3 bits (1118), Expect = 8.3e-122
Identity = 243/580 (41.90%), Postives = 360/580 (62.07%), Query Frame = 0

Query: 33  CNEQCGNLKIPYPFGIKEGCYH--NENFSITCNKTRYNPPKAFLKDTDIGITNISII--- 92
           C  +CG++ I YPFGI  GCY+  +++F+ITC + + N            ++NI ++   
Sbjct: 29  CQTRCGDVPIDYPFGISTGCYYPGDDSFNITCEEDKPNV-----------LSNIEVLNFN 88

Query: 93  -EGQLPILQSIAKDCYTKNGTPESNDPTFQLFNFTISNTMNKFTVIGCDTYAFISGELEG 152
             GQL  L   +  CY +    +     F+L N + S   NKFT++GC+ +A +S     
Sbjct: 89  HSGQLRGLIPRSTVCYDQQTNNDFESLWFRLDNLSFSPN-NKFTLVGCNAWALLS-TFGI 148

Query: 153 ESYISGCMALCGNSTETIRDGSCSGSGCCQLE--IPRGLKNLDVEVKSFDNHSKVLNFNP 212
           ++Y +GCM+LC   T    +  C+G GCC+ E  IP     ++ +   F+N + V +FNP
Sbjct: 149 QNYSTGCMSLC--DTPPPPNSKCNGVGCCRTEVSIPLDSHRIETQPSRFENMTSVEHFNP 208

Query: 213 CGYAFVIQQDKFNFST----NYIRNFTQKEVPLVLDWAIPTNKSCLEQAKNKSNCSICGL 272
           C YAF ++   FNFS+      +RN T+   P++LDW+I  N++C EQ   +   +ICG 
Sbjct: 209 CSYAFFVEDGMFNFSSLEDLKDLRNVTR--FPVLLDWSI-GNQTC-EQVVGR---NICGG 268

Query: 273 NTKRINFLDDGSEYRCQCLEGFEGNPYLPQGCQDIDECKNGSQKCKYKELCVNTPGNYTC 332
           N+   +    G  Y C+CL+GF+GNPYL  GCQDI+EC      C     C NT G++ C
Sbjct: 269 NSTCFD-STRGKGYNCKCLQGFDGNPYLSDGCQDINECTTRIHNCSDTSTCENTLGSFHC 328

Query: 333 HCPKNH-------------KGDGRYGGEGCIPNSISSIHIIIVFVFGSIWLYLGYKKWKF 392
            CP                K + +Y G   +    +   +II+     I   + ++  K 
Sbjct: 329 QCPSGSDLNTTTMSCIDTPKEEPKYLGWTTVLLGTTIGFLIILLTISYIQQKMRHR--KN 388

Query: 393 IQQKEKFFKKNGGLMLRQHLSQWQSPDM-LKIFTQEELEKATNKYDEKAVVGKGGYGTVY 452
            + +++FF++NGG ML Q LS     ++ +KIFT+E +++AT+ Y+E  ++G+GG GTVY
Sbjct: 389 TELRQQFFEQNGGGMLIQRLSGAGPSNVDVKIFTEEGMKEATDGYNESRILGQGGQGTVY 448

Query: 453 KGVLDNGLTVAIKKSKLVNQSQIDQFINEVNVLSQINHRNVVKLIWCCLETKVPLLVYEL 512
           KG+L +   VAIKK++L ++SQ++QFINEV VLSQINHRNVVKL+ CCLET+VPLLVYE 
Sbjct: 449 KGILQDNSIVAIKKARLGDRSQVEQFINEVLVLSQINHRNVVKLLGCCLETEVPLLVYEF 508

Query: 513 VTNGTLFEHIHRKTNQVYLPWEARLKIASEIASVVSYLHSSASTPIIHRDIKSSNILLDQ 572
           +++GTLF+H+H       L WE RL+IA E+A  ++YLHS AS PIIHRD+K++NILLD+
Sbjct: 509 ISSGTLFDHLHGSMFDSSLTWEHRLRIAIEVAGTLAYLHSYASIPIIHRDVKTANILLDE 568

Query: 573 NYTAKISDFGTSKLVPLDRTQISTMVQGTIGYLDPEYFLT 587
           N TAK++DFG S+L+P+D+ Q++TMVQGT+GYLDPEY+ T
Sbjct: 569 NLTAKVADFGASRLIPMDQEQLTTMVQGTLGYLDPEYYNT 583

BLAST of Lsi08G004970 vs. TAIR 10
Match: AT1G21210.1 (wall associated kinase 4 )

HSP 1 Score: 400.2 bits (1027), Expect = 3.0e-111
Identity = 236/602 (39.20%), Postives = 349/602 (57.97%), Query Frame = 0

Query: 7   KLIKLIILSSATLFVVAAAVKASFTGCNEQCGNLKIPYPFGIKEGCYHNENFSITCNKTR 66
           K+ +L +++   L  +      +   C E+CGN+ + YPFG   GC+  E+ S   +   
Sbjct: 2   KVQRLFLVAIFCLSYMQLVKGQTLPRCPEKCGNVTLEYPFGFSPGCWRAEDPSFNLSCVN 61

Query: 67  YNPPKAFLKDTDIGITNISIIEGQLPILQSIAKDCYTKNGT-PESNDPTFQLFNFTISNT 126
            N    F K  ++   + S    QL +L   +  CY   G   +       L N T+S  
Sbjct: 62  EN---LFYKGLEVVEISHS---SQLRVLYPASYICYNSKGKFAKGTYYWSNLGNLTLSGN 121

Query: 127 MNKFTVIGCDTYAFISGELEGESYISGCMALCGNSTETIRDGSCSGSGCCQLEIPRGLKN 186
            N  T +GC++YAF+S      + + GC++ C ++     +G C+G GCCQ  +P G   
Sbjct: 122 -NTITALGCNSYAFVSSNGTRRNSV-GCISAC-DALSHEANGECNGEGCCQNPVPAGNNW 181

Query: 187 LDVEVKSFDNHSKV--LNFNPCGYAFVIQQDKFNFSTNYIRNFTQKE---VPLVLDWAIP 246
           L V    FDN + V  ++   C YAF+++  KF ++ +   ++ Q      P+VLDW+I 
Sbjct: 182 LIVRSYRFDNDTSVQPISEGQCIYAFLVENGKFKYNASDKYSYLQNRNVGFPVVLDWSI- 241

Query: 247 TNKSCLEQAKNKSNCSICGLNTKRINFLDDGSEYRCQCLEGFEGNPYLPQGCQDIDECKN 306
             ++C +  + K     CG+N    N    G  Y C+C  GF+GNPYL  GCQDI+EC  
Sbjct: 242 RGETCGQVGEKK-----CGVNGICSNSA-SGIGYTCKCKGGFQGNPYLQNGCQDINECTT 301

Query: 307 GS----QKCKYKELCVNTPGNYTCHCPKNH-----------KGDGRYGGEGCIPNSISSI 366
            +      C     C N  G++ C+C   +           KG+  Y     I    +  
Sbjct: 302 ANPIHKHNCSGDSTCENKLGHFRCNCRSRYELNTTTNTCKPKGNPEYVEWTTIVLGTTIG 361

Query: 367 HIIIVFVFGSIWLYLGYKKWKFIQQKEKFFKKNGGLMLRQHLSQWQSPDM-LKIFTQEEL 426
            ++I+     I   +  K  K  + +++FF++NGG ML Q LS     ++ +KIFT+E +
Sbjct: 362 FLVILLAISCIEHKM--KNTKDTELRQQFFEQNGGGMLMQRLSGAGPSNVDVKIFTEEGM 421

Query: 427 EKATNKYDEKAVVGKGGYGTVYKGVLDNGLTVAIKKSKLVNQSQIDQFINEVNVLSQINH 486
           ++AT+ YDE  ++G+GG GTVYKG+L +   VAIKK++L + SQ++QFINEV VLSQINH
Sbjct: 422 KEATDGYDENRILGQGGQGTVYKGILPDNSIVAIKKARLGDNSQVEQFINEVLVLSQINH 481

Query: 487 RNVVKLIWCCLETKVPLLVYELVTNGTLFEHIHRKTNQVYLPWEARLKIASEIASVVSYL 546
           RNVVKL+ CCLET+VPLLVYE +++GTLF+H+H       L WE RL++A EIA  ++YL
Sbjct: 482 RNVVKLLGCCLETEVPLLVYEFISSGTLFDHLHGSMFDSSLTWEHRLRMAVEIAGTLAYL 541

Query: 547 HSSASTPIIHRDIKSSNILLDQNYTAKISDFGTSKLVPLDRTQISTMVQGTIGYLDPEYF 587
           HSSAS PIIHRDIK++NILLD+N TAK++DFG S+L+P+D+  ++TMVQGT+GYLDPEY+
Sbjct: 542 HSSASIPIIHRDIKTANILLDENLTAKVADFGASRLIPMDKEDLATMVQGTLGYLDPEYY 585

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LMP16.6e-13245.12Wall-associated receptor kinase 2 OS=Arabidopsis thaliana OX=3702 GN=WAK2 PE=1 S... [more]
Q391917.8e-12543.21Wall-associated receptor kinase 1 OS=Arabidopsis thaliana OX=3702 GN=WAK1 PE=1 S... [more]
Q9LMN81.4e-12142.59Wall-associated receptor kinase 3 OS=Arabidopsis thaliana OX=3702 GN=WAK3 PE=2 S... [more]
Q9LMN71.2e-12041.90Wall-associated receptor kinase 5 OS=Arabidopsis thaliana OX=3702 GN=WAK5 PE=2 S... [more]
Q9LMN64.2e-11039.20Wall-associated receptor kinase 4 OS=Arabidopsis thaliana OX=3702 GN=WAK4 PE=2 S... [more]
Match NameE-valueIdentityDescription
A0A5D3DKI34.3e-27578.73Wall-associated receptor kinase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3B3R74.3e-27578.73wall-associated receptor kinase 2-like OS=Cucumis melo OX=3656 GN=LOC103485671 P... [more]
A0A6J1CJM07.9e-22164.44putative wall-associated receptor kinase-like 16 OS=Momordica charantia OX=3673 ... [more]
A0A6J1H8431.8e-22064.93wall-associated receptor kinase 3-like OS=Cucurbita moschata OX=3662 GN=LOC11146... [more]
A0A6J1JNA51.9e-21964.53wall-associated receptor kinase 2-like OS=Cucurbita maxima OX=3661 GN=LOC1114868... [more]
Match NameE-valueIdentityDescription
XP_038885912.15.3e-28078.99wall-associated receptor kinase 2-like [Benincasa hispida][more]
XP_008441592.18.8e-27578.73PREDICTED: wall-associated receptor kinase 2-like [Cucumis melo] >TYK23789.1 wal... [more]
XP_038886240.14.6e-23165.78wall-associated receptor kinase 2-like [Benincasa hispida][more]
XP_022141581.11.6e-22064.44putative wall-associated receptor kinase-like 16 [Momordica charantia][more]
XP_022960687.13.6e-22064.93wall-associated receptor kinase 3-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT1G21270.14.7e-13345.12wall-associated kinase 2 [more]
AT1G21250.15.6e-12643.21cell wall-associated kinase [more]
AT1G21240.19.8e-12342.59wall associated kinase 3 [more]
AT1G21230.18.3e-12241.90wall associated kinase 5 [more]
AT1G21210.13.0e-11139.20wall associated kinase 4 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000742EGF-like domainSMARTSM00181egf_5coord: 297..338
e-value: 23.0
score: 11.7
coord: 244..293
e-value: 8.3
score: 15.4
IPR000742EGF-like domainPROSITEPS50026EGF_3coord: 294..338
score: 10.943007
IPR001881EGF-like calcium-binding domainSMARTSM00179egfca_6coord: 294..338
e-value: 4.7E-4
score: 28.7
IPR001881EGF-like calcium-binding domainPFAMPF07645EGF_CAcoord: 294..327
e-value: 4.3E-7
score: 30.0
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 411..606
e-value: 1.1E-11
score: 33.0
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 411..588
e-value: 4.8E-37
score: 127.8
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 411..609
score: 31.283501
NoneNo IPR availableGENE3D2.10.25.10Laminincoord: 242..337
e-value: 1.2E-11
score: 46.8
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 487..601
e-value: 3.2E-31
score: 110.3
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 384..486
e-value: 7.1E-29
score: 102.0
NoneNo IPR availablePIRSRPIRSR000556-2PIRSR000556-2coord: 409..582
e-value: 1.0E-18
score: 65.1
NoneNo IPR availablePANTHERPTHR27005:SF341WALL-ASSOCIATED RECEPTOR KINASE 2-LIKE ISOFORM X2coord: 17..587
NoneNo IPR availablePANTHERPTHR27005WALL-ASSOCIATED RECEPTOR KINASE-LIKE 21coord: 17..587
NoneNo IPR availableCDDcd00054EGF_CAcoord: 294..329
e-value: 1.17606E-5
score: 40.6978
NoneNo IPR availableSUPERFAMILY57196EGF/Laminincoord: 293..329
IPR025287Wall-associated receptor kinase, galacturonan-binding domainPFAMPF13947GUB_WAK_bindcoord: 33..86
e-value: 4.9E-9
score: 36.5
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 417..440
IPR000152EGF-type aspartate/asparagine hydroxylation sitePROSITEPS00010ASX_HYDROXYLcoord: 311..322
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 532..544
IPR018097EGF-like calcium-binding, conserved sitePROSITEPS01187EGF_CAcoord: 294..320
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 388..587

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi08G004970.1Lsi08G004970.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007166 cell surface receptor signaling pathway
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0030247 polysaccharide binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity