Lsi08G004660 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi08G004660
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionHistone-lysine N-methyltransferase ASHH2
Locationchr08: 12382357 .. 12383697 (+)
RNA-Seq ExpressionLsi08G004660
SyntenyLsi08G004660
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCATGTCTTGGCGTAGGTATGATCTTTGGCCAAAAGCTTTCAAAGTTCAGTTTTGACGAGTTAAGCATGAACCTGCAGGTACATCAAATTGCCCGAAGTTTTCGGGACAGATGGTTCCCCAGACATACTAGAAAATTTGGTTACTCTGAGAGGGAGGATGGAAGATTGGAAGGTTACAGGGGTTCAAACTGCGGTAGGTTTACAGCATCACACAGTTATCGGCATGATCAGGATTCCAGACCGACGGATGCAATTGATTGTGTCAAGCAGTCAATGCCATCTTTGCCAGATGCTCATCCTGCGGAGGTCTGTTCTGTGGCTTCCACAGCTGGTCACTCATTGAATGGACAAAAAGTCCGTAAGCGTAAGAGCCGATGGGATCAGCCTGCAGACACAAGCCTAGATCTGAGATCCAAGGAGCAGAAGCTTGAATCAACATCAGTGCAGCAATTGAATTCCAGCCAATTAAATGATGTTGGAGTGGCGTCAATGTTGATAGACAAAGTAAACAATGATGATAAGGACCCCTCCCTCTCTGATTCTGTTGGAGATCGTTGTCGGCAAGATGAAGATATTAGGGCAGACAGTGCAGTGCAGAACATCCTTGAAGATATTCCTCCAGGATTTTCATCTCCCTTTCATCCCCCCATGGCTTCCTCGAGCACTTTTTCAACAGTTTTAGATCCTCCTCGTCAGAATATTGGCAATTTGAGTTGTGCTTTTTCCACGGTTGGGCACCCACAGGAAAGATTCATTTCTCGCTTGCCTGTGTCCTATGGAATTCCATTTTCTATCATTGAGCAATGTGGAACATCCCGTGCAGAGAATCTGGAGTGTTGGGATGTTGCTCCAGGAGTGCCTTTTCATCCTTTTCCACCCCTGCCGCCTTATCCCCGAGGTAAGAGAGGCCCGCCTACATCTGCCTGTGGTACTGTAGTTGGACAGTCCTTTCAAGAAGGGCAGGTCAACAGCCATGATTCTCGAACTTCTTTCTCAGAAGAAAGCCCTCCTAGTACAAGTACTAATTACCAACCGGATGTGTGCACTCCATCAAACAACCAACAGATACCGAAACGGGCAAAAGAATCATCGTACGACTTGGGAAGAAGGTACTTTAGGCAGCAAAAGTGGCGTAATTCAAAGTATGGCCCCCCTTGGTTACAGAGAAGAAATCAGTGGGGATGCCAGGGGAACTTCAGGGAAGGGGTGAGCACTATAGGTGATGACAATATACCCAATGAAGAAATAAGTCCATATTACTCGGATGAAGCAAGCGGTAAAGTGGATAAAGCTAATGATGATTTTTATCAGCATTTGCAGAACCAAAATCTGCATTGA

mRNA sequence

ATGGCATGTCTTGGCGTAGGTATGATCTTTGGCCAAAAGCTTTCAAAGTTCAGTTTTGACGAGTTAAGCATGAACCTGCAGGTACATCAAATTGCCCGAAGTTTTCGGGACAGATGGTTCCCCAGACATACTAGAAAATTTGGTTACTCTGAGAGGGAGGATGGAAGATTGGAAGGTTACAGGGGTTCAAACTGCGGTAGGTTTACAGCATCACACAGTTATCGGCATGATCAGGATTCCAGACCGACGGATGCAATTGATTGTGTCAAGCAGTCAATGCCATCTTTGCCAGATGCTCATCCTGCGGAGGTCTGTTCTGTGGCTTCCACAGCTGGTCACTCATTGAATGGACAAAAAGTCCGTAAGCGTAAGAGCCGATGGGATCAGCCTGCAGACACAAGCCTAGATCTGAGATCCAAGGAGCAGAAGCTTGAATCAACATCAGTGCAGCAATTGAATTCCAGCCAATTAAATGATGTTGGAGTGGCGTCAATGTTGATAGACAAAGTAAACAATGATGATAAGGACCCCTCCCTCTCTGATTCTGTTGGAGATCGTTGTCGGCAAGATGAAGATATTAGGGCAGACAGTGCAGTGCAGAACATCCTTGAAGATATTCCTCCAGGATTTTCATCTCCCTTTCATCCCCCCATGGCTTCCTCGAGCACTTTTTCAACAGTTTTAGATCCTCCTCGTCAGAATATTGGCAATTTGAGTTGTGCTTTTTCCACGGTTGGGCACCCACAGGAAAGATTCATTTCTCGCTTGCCTGTGTCCTATGGAATTCCATTTTCTATCATTGAGCAATGTGGAACATCCCGTGCAGAGAATCTGGAGTGTTGGGATGTTGCTCCAGGAGTGCCTTTTCATCCTTTTCCACCCCTGCCGCCTTATCCCCGAGGTAAGAGAGGCCCGCCTACATCTGCCTGTGGTACTGTAGTTGGACAGTCCTTTCAAGAAGGGCAGGTCAACAGCCATGATTCTCGAACTTCTTTCTCAGAAGAAAGCCCTCCTAGTACAAGTACTAATTACCAACCGGATGTGTGCACTCCATCAAACAACCAACAGATACCGAAACGGGCAAAAGAATCATCGTACGACTTGGGAAGAAGGTACTTTAGGCAGCAAAAGTGGCGTAATTCAAAGTATGGCCCCCCTTGGTTACAGAGAAGAAATCAGTGGGGATGCCAGGGGAACTTCAGGGAAGGGGTGAGCACTATAGGTGATGACAATATACCCAATGAAGAAATAAGTCCATATTACTCGGATGAAGCAAGCGGTAAAGTGGATAAAGCTAATGATGATTTTTATCAGCATTTGCAGAACCAAAATCTGCATTGA

Coding sequence (CDS)

ATGGCATGTCTTGGCGTAGGTATGATCTTTGGCCAAAAGCTTTCAAAGTTCAGTTTTGACGAGTTAAGCATGAACCTGCAGGTACATCAAATTGCCCGAAGTTTTCGGGACAGATGGTTCCCCAGACATACTAGAAAATTTGGTTACTCTGAGAGGGAGGATGGAAGATTGGAAGGTTACAGGGGTTCAAACTGCGGTAGGTTTACAGCATCACACAGTTATCGGCATGATCAGGATTCCAGACCGACGGATGCAATTGATTGTGTCAAGCAGTCAATGCCATCTTTGCCAGATGCTCATCCTGCGGAGGTCTGTTCTGTGGCTTCCACAGCTGGTCACTCATTGAATGGACAAAAAGTCCGTAAGCGTAAGAGCCGATGGGATCAGCCTGCAGACACAAGCCTAGATCTGAGATCCAAGGAGCAGAAGCTTGAATCAACATCAGTGCAGCAATTGAATTCCAGCCAATTAAATGATGTTGGAGTGGCGTCAATGTTGATAGACAAAGTAAACAATGATGATAAGGACCCCTCCCTCTCTGATTCTGTTGGAGATCGTTGTCGGCAAGATGAAGATATTAGGGCAGACAGTGCAGTGCAGAACATCCTTGAAGATATTCCTCCAGGATTTTCATCTCCCTTTCATCCCCCCATGGCTTCCTCGAGCACTTTTTCAACAGTTTTAGATCCTCCTCGTCAGAATATTGGCAATTTGAGTTGTGCTTTTTCCACGGTTGGGCACCCACAGGAAAGATTCATTTCTCGCTTGCCTGTGTCCTATGGAATTCCATTTTCTATCATTGAGCAATGTGGAACATCCCGTGCAGAGAATCTGGAGTGTTGGGATGTTGCTCCAGGAGTGCCTTTTCATCCTTTTCCACCCCTGCCGCCTTATCCCCGAGGTAAGAGAGGCCCGCCTACATCTGCCTGTGGTACTGTAGTTGGACAGTCCTTTCAAGAAGGGCAGGTCAACAGCCATGATTCTCGAACTTCTTTCTCAGAAGAAAGCCCTCCTAGTACAAGTACTAATTACCAACCGGATGTGTGCACTCCATCAAACAACCAACAGATACCGAAACGGGCAAAAGAATCATCGTACGACTTGGGAAGAAGGTACTTTAGGCAGCAAAAGTGGCGTAATTCAAAGTATGGCCCCCCTTGGTTACAGAGAAGAAATCAGTGGGGATGCCAGGGGAACTTCAGGGAAGGGGTGAGCACTATAGGTGATGACAATATACCCAATGAAGAAATAAGTCCATATTACTCGGATGAAGCAAGCGGTAAAGTGGATAAAGCTAATGATGATTTTTATCAGCATTTGCAGAACCAAAATCTGCATTGA

Protein sequence

MACLGVGMIFGQKLSKFSFDELSMNLQVHQIARSFRDRWFPRHTRKFGYSEREDGRLEGYRGSNCGRFTASHSYRHDQDSRPTDAIDCVKQSMPSLPDAHPAEVCSVASTAGHSLNGQKVRKRKSRWDQPADTSLDLRSKEQKLESTSVQQLNSSQLNDVGVASMLIDKVNNDDKDPSLSDSVGDRCRQDEDIRADSAVQNILEDIPPGFSSPFHPPMASSSTFSTVLDPPRQNIGNLSCAFSTVGHPQERFISRLPVSYGIPFSIIEQCGTSRAENLECWDVAPGVPFHPFPPLPPYPRGKRGPPTSACGTVVGQSFQEGQVNSHDSRTSFSEESPPSTSTNYQPDVCTPSNNQQIPKRAKESSYDLGRRYFRQQKWRNSKYGPPWLQRRNQWGCQGNFREGVSTIGDDNIPNEEISPYYSDEASGKVDKANDDFYQHLQNQNLH
Homology
BLAST of Lsi08G004660 vs. ExPASy Swiss-Prot
Match: Q2LAE1 (Histone-lysine N-methyltransferase ASHH2 OS=Arabidopsis thaliana OX=3702 GN=ASHH2 PE=1 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 3.3e-32
Identity = 131/389 (33.68%), Postives = 166/389 (42.67%), Query Frame = 0

Query: 13   KLSKFSFDELSMNLQVHQIARSFRDRWFPRHTRKFGYSEREDGRLEGYRGSNCGRFTASH 72
            K S  SF E   +  VH IARSFRDRW P+H RK     RE+ R E  R     RF AS 
Sbjct: 1456 KDSVLSFTEHD-DYTVHNIARSFRDRWIPKHFRKPWRINREE-RSESMRSPINRRFRASQ 1515

Query: 73   SYRHD-QDSRPTDAIDCVKQSMPSLPDAHPAEVCSVASTAGHSLNGQKVRKRKSRWDQPA 132
              R+D Q  RP +    V  S  + P+   A V    S     L     RKRKSRWDQP+
Sbjct: 1516 EPRYDHQSPRPAEPAASVTSSKAATPET--ASVSEGYSEPNSGLPETNGRKRKSRWDQPS 1575

Query: 133  DTSLDLRSKEQKLESTSVQQLNSSQLNDVGVASMLIDKVNNDDKDPSLSDSVGDRCRQDE 192
             T      KEQ++ +   QQ + +  N                                 
Sbjct: 1576 KT------KEQRIMTILSQQTDETNGN--------------------------------- 1635

Query: 193  DIRADSAVQNILEDIPPGFSSPFHPPMASSSTFSTVLDPPRQNIGNLSCAFSTVGHPQER 252
                    Q++ +D+PPGFSSP               D P           +    PQ++
Sbjct: 1636 --------QDVQDDLPPGFSSP-------------CTDVPD----------AITAQPQQK 1695

Query: 253  FISRLPVSYGIPFSIIEQCGTSRAENLECWDVAPGVPFHPFPPLPPYPRG----KRGPPT 312
            F+SRLPVSYGIP SI+ Q G+   E+   W VAPG+PF+PFPPLPP   G    KR    
Sbjct: 1696 FLSRLPVSYGIPLSIVHQFGSPGKEDPTTWSVAPGMPFYPFPPLPPVSHGEFFAKRN--V 1739

Query: 313  SACGTVVGQSFQEGQVNSHDSRTSFSEESPPSTSTNYQPDVCTPSNNQQIPKRAKE-SSY 372
             AC + +G               ++S E  P+          TP  +   P R +E  S 
Sbjct: 1756 RACSSSMG-------------NLTYSNEILPA----------TPVTDSTAPTRKRELFSS 1739

Query: 373  DLGRRYFRQQKWRNSKYGPPWLQRRNQWG 396
            D+G  YFRQQK    +  PPWL  RN  G
Sbjct: 1816 DIGTTYFRQQK----QSVPPWL--RNNGG 1739

BLAST of Lsi08G004660 vs. ExPASy TrEMBL
Match: A0A0A0KDR4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G376290 PE=4 SV=1)

HSP 1 Score: 749.6 bits (1934), Expect = 7.6e-213
Identity = 376/420 (89.52%), Postives = 391/420 (93.10%), Query Frame = 0

Query: 27   QVHQIARSFRDRWFPRHTRKFGYSEREDGRLEGYRGSNCGRFTASHSYRHDQDSRPTDAI 86
            QVHQIARSFRDRWFPRHTRKFGYSEREDGRLE YRGSN  RFTASHS+RHDQD RPTDAI
Sbjct: 1732 QVHQIARSFRDRWFPRHTRKFGYSEREDGRLEVYRGSNSSRFTASHSFRHDQDCRPTDAI 1791

Query: 87   DCVKQSMP-SLPDAHPAEVCSVASTAGHSLNGQKVRKRKSRWDQPADTSLDLRSKEQKLE 146
            DC+KQSMP SLPDAHPAEVCS+AS A HS+NGQKVRKRKSRWDQPADTSLDLRSKEQKLE
Sbjct: 1792 DCIKQSMPTSLPDAHPAEVCSLASAASHSVNGQKVRKRKSRWDQPADTSLDLRSKEQKLE 1851

Query: 147  STSVQQLNSSQLNDVGVASMLIDKVNNDDKDPSLSDSVGDRCRQDEDIRADSAVQNILED 206
            STSVQ+LNSSQLN VG ASMLIDKVNNDDKD SLSDSVG  CRQDEDIRADSAV NI ED
Sbjct: 1852 STSVQELNSSQLNSVGAASMLIDKVNNDDKDISLSDSVGVPCRQDEDIRADSAVPNIPED 1911

Query: 207  IPPGFSSPFHPPMASSSTFSTVLDPPRQNIGNLSCAFSTVGHPQERFISRLPVSYGIPFS 266
            IPPGFSSPF+PP+ASSS FS VLDPPRQNIG+LSCAFSTVGH QERFISRLPVSYGIPFS
Sbjct: 1912 IPPGFSSPFNPPVASSSAFSAVLDPPRQNIGDLSCAFSTVGHLQERFISRLPVSYGIPFS 1971

Query: 267  IIEQCGTSRAENLECWDVAPGVPFHPFPPLPPYPRGKRGPPTSACGTVVGQSFQEGQVNS 326
            IIEQCGTS AENLECWDVAPGVPFHPFPPLPPYPRG RG PTSACGT  GQS QEGQVNS
Sbjct: 1972 IIEQCGTSHAENLECWDVAPGVPFHPFPPLPPYPRGMRGLPTSACGT-AGQSSQEGQVNS 2031

Query: 327  HDSRTSFSEESPPSTSTNYQPDVCTPSNNQQIPKRAKESSYDLGRRYFRQQKWRNSKYGP 386
            HDSRTSFSEESPPSTSTNYQ D+CTPSNNQQI KRAKESS DLGRRYFRQQKWRN+K+GP
Sbjct: 2032 HDSRTSFSEESPPSTSTNYQTDLCTPSNNQQIAKRAKESSCDLGRRYFRQQKWRNTKFGP 2091

Query: 387  PWLQRRNQWGCQGNFREGVSTIGDDNIPNEEISPYYSDEASGKVDKANDDFYQHLQNQNL 446
            PWLQRR+QWGCQGNFR GVSTIGD+NIP+EEISPY SDEASG+VDKAN DFYQHLQNQNL
Sbjct: 2092 PWLQRRSQWGCQGNFRGGVSTIGDENIPDEEISPYCSDEASGRVDKANGDFYQHLQNQNL 2150

BLAST of Lsi08G004660 vs. ExPASy TrEMBL
Match: A0A5A7UPQ6 (Histone-lysine N-methyltransferase ASHH2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold221G001140 PE=4 SV=1)

HSP 1 Score: 734.9 bits (1896), Expect = 1.9e-208
Identity = 371/420 (88.33%), Postives = 386/420 (91.90%), Query Frame = 0

Query: 27   QVHQIARSFRDRWFPRHTRKFGYSEREDGRLEGYRGSNCGRFTASHSYRHDQDSRPTDAI 86
            QVHQIARSFRDRWFPRHTRKFGYSEREDGRLE YRGSN  RFTASHSYRHDQD RPTDAI
Sbjct: 1692 QVHQIARSFRDRWFPRHTRKFGYSEREDGRLEVYRGSNSSRFTASHSYRHDQDCRPTDAI 1751

Query: 87   DCVKQSMPS-LPDAHPAEVCSVASTAGHSLNGQKVRKRKSRWDQPADTSLDLRSKEQKLE 146
            DC+KQSMP+ LPDAH AEVCS+AS AG S+NGQKVRKRKSRWDQPADTSLDLRSKEQKLE
Sbjct: 1752 DCIKQSMPTPLPDAHTAEVCSLASVAGPSVNGQKVRKRKSRWDQPADTSLDLRSKEQKLE 1811

Query: 147  STSVQQLNSSQLNDVGVASMLIDKVNNDDKDPSLSDSVGDRCRQDEDIRADSAVQNILED 206
            STSVQ+LNSSQLN V VASMLIDKVNNDDKD SLSDSVG  CRQDED RADSAV NI ED
Sbjct: 1812 STSVQELNSSQLNSVRVASMLIDKVNNDDKDSSLSDSVGVPCRQDEDTRADSAVPNIPED 1871

Query: 207  IPPGFSSPFHPPMASSSTFSTVLDPPRQNIGNLSCAFSTVGHPQERFISRLPVSYGIPFS 266
            IPPGFSSPF+P +ASSS FS VLDPP+QNIG LSCAFSTVGH QERFISRLPVSYGIPFS
Sbjct: 1872 IPPGFSSPFNPSVASSSAFSAVLDPPQQNIGYLSCAFSTVGHLQERFISRLPVSYGIPFS 1931

Query: 267  IIEQCGTSRAENLECWDVAPGVPFHPFPPLPPYPRGKRGPPTSACGTVVGQSFQEGQVNS 326
            IIEQCGTSRAENLECWDVAPGVPFHPFPPLPPYPRG  GP TSACGT  GQS QEGQVNS
Sbjct: 1932 IIEQCGTSRAENLECWDVAPGVPFHPFPPLPPYPRGMSGPRTSACGT-AGQSSQEGQVNS 1991

Query: 327  HDSRTSFSEESPPSTSTNYQPDVCTPSNNQQIPKRAKESSYDLGRRYFRQQKWRNSKYGP 386
            HDSRTSFSEESPPSTSTNYQ D+CTPSNNQQI KR KESS DLGRRYFRQQKWRN+K+GP
Sbjct: 1992 HDSRTSFSEESPPSTSTNYQTDLCTPSNNQQITKRPKESSCDLGRRYFRQQKWRNTKFGP 2051

Query: 387  PWLQRRNQWGCQGNFREGVSTIGDDNIPNEEISPYYSDEASGKVDKANDDFYQHLQNQNL 446
            PWLQRR+QWGCQGNFR GVSTIGD+NIP+EEISPY SDEASG+VDKAN DFYQHLQNQNL
Sbjct: 2052 PWLQRRSQWGCQGNFRGGVSTIGDENIPDEEISPYCSDEASGRVDKANGDFYQHLQNQNL 2110

BLAST of Lsi08G004660 vs. ExPASy TrEMBL
Match: A0A1S3B3U9 (LOW QUALITY PROTEIN: histone-lysine N-methyltransferase ASHH2 OS=Cucumis melo OX=3656 GN=LOC103485694 PE=4 SV=1)

HSP 1 Score: 734.9 bits (1896), Expect = 1.9e-208
Identity = 371/420 (88.33%), Postives = 386/420 (91.90%), Query Frame = 0

Query: 27   QVHQIARSFRDRWFPRHTRKFGYSEREDGRLEGYRGSNCGRFTASHSYRHDQDSRPTDAI 86
            QVHQIARSFRDRWFPRHTRKFGYSEREDGRLE YRGSN  RFTASHSYRHDQD RPTDAI
Sbjct: 1663 QVHQIARSFRDRWFPRHTRKFGYSEREDGRLEVYRGSNSSRFTASHSYRHDQDCRPTDAI 1722

Query: 87   DCVKQSMPS-LPDAHPAEVCSVASTAGHSLNGQKVRKRKSRWDQPADTSLDLRSKEQKLE 146
            DC+KQSMP+ LPDAH AEVCS+AS AG S+NGQKVRKRKSRWDQPADTSLDLRSKEQKLE
Sbjct: 1723 DCIKQSMPTPLPDAHTAEVCSLASVAGPSVNGQKVRKRKSRWDQPADTSLDLRSKEQKLE 1782

Query: 147  STSVQQLNSSQLNDVGVASMLIDKVNNDDKDPSLSDSVGDRCRQDEDIRADSAVQNILED 206
            STSVQ+LNSSQLN V VASMLIDKVNNDDKD SLSDSVG  CRQDED RADSAV NI ED
Sbjct: 1783 STSVQELNSSQLNSVRVASMLIDKVNNDDKDSSLSDSVGVPCRQDEDTRADSAVPNIPED 1842

Query: 207  IPPGFSSPFHPPMASSSTFSTVLDPPRQNIGNLSCAFSTVGHPQERFISRLPVSYGIPFS 266
            IPPGFSSPF+P +ASSS FS VLDPP+QNIG LSCAFSTVGH QERFISRLPVSYGIPFS
Sbjct: 1843 IPPGFSSPFNPSVASSSAFSAVLDPPQQNIGYLSCAFSTVGHLQERFISRLPVSYGIPFS 1902

Query: 267  IIEQCGTSRAENLECWDVAPGVPFHPFPPLPPYPRGKRGPPTSACGTVVGQSFQEGQVNS 326
            IIEQCGTSRAENLECWDVAPGVPFHPFPPLPPYPRG  GP TSACGT  GQS QEGQVNS
Sbjct: 1903 IIEQCGTSRAENLECWDVAPGVPFHPFPPLPPYPRGMSGPRTSACGT-AGQSSQEGQVNS 1962

Query: 327  HDSRTSFSEESPPSTSTNYQPDVCTPSNNQQIPKRAKESSYDLGRRYFRQQKWRNSKYGP 386
            HDSRTSFSEESPPSTSTNYQ D+CTPSNNQQI KR KESS DLGRRYFRQQKWRN+K+GP
Sbjct: 1963 HDSRTSFSEESPPSTSTNYQTDLCTPSNNQQITKRPKESSCDLGRRYFRQQKWRNTKFGP 2022

Query: 387  PWLQRRNQWGCQGNFREGVSTIGDDNIPNEEISPYYSDEASGKVDKANDDFYQHLQNQNL 446
            PWLQRR+QWGCQGNFR GVSTIGD+NIP+EEISPY SDEASG+VDKAN DFYQHLQNQNL
Sbjct: 2023 PWLQRRSQWGCQGNFRGGVSTIGDENIPDEEISPYCSDEASGRVDKANGDFYQHLQNQNL 2081

BLAST of Lsi08G004660 vs. ExPASy TrEMBL
Match: A0A6J1JXF7 (histone-lysine N-methyltransferase ASHH2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111489728 PE=4 SV=1)

HSP 1 Score: 690.6 bits (1781), Expect = 4.2e-195
Identity = 355/419 (84.73%), Postives = 374/419 (89.26%), Query Frame = 0

Query: 27   QVHQIARSFRDRWFPRHTRKFGYSEREDGRLEGYRGSNCGRFTASHSYRHDQDSRPTDAI 86
            QVHQIARSFRDRWFPRHTRKF YSEREDGRLE YRGSNC RFTASHSYR DQDSRPTDAI
Sbjct: 1670 QVHQIARSFRDRWFPRHTRKFVYSEREDGRLEVYRGSNCSRFTASHSYRRDQDSRPTDAI 1729

Query: 87   DCVKQSM-PSLPDAHPAEVCSVASTAGHSLNGQKVRKRKSRWDQPADTSLDLRSKEQKLE 146
            DCVKQS+  SLPDAHPAEVCS+ASTAGHSLNGQKV KRKSRWDQPADTSLDLRSKEQKLE
Sbjct: 1730 DCVKQSLSTSLPDAHPAEVCSMASTAGHSLNGQKVCKRKSRWDQPADTSLDLRSKEQKLE 1789

Query: 147  STSVQQLNSSQLNDVGVASMLIDKVNNDDKDPSLSDSVGDRCRQDEDIRADSAVQNILED 206
            S SVQQ NSSQL+ VGV SMLIDKVN+DDKD SLSDSVG R  QDEDIRADSAVQNI ED
Sbjct: 1790 SKSVQQFNSSQLSSVGVVSMLIDKVNSDDKDFSLSDSVGVRGSQDEDIRADSAVQNIPED 1849

Query: 207  IPPGFSSPFHPPMASSSTFSTVLDPPRQNIGNLSCAFSTVGHPQERFISRLPVSYGIPFS 266
            IPPGF  PF  P+ASSS FSTVLDPPRQ+IG LSCAFSTVG+PQE+FIS LPVSYGIPFS
Sbjct: 1850 IPPGF-FPFSLPVASSSAFSTVLDPPRQSIGKLSCAFSTVGYPQEKFISCLPVSYGIPFS 1909

Query: 267  IIEQCGTSRAENLECWDVAPGVPFHPFPPLPPYPRGKRGPPTSACGTVVGQSFQEGQVNS 326
            I+EQCGTS AENLECWDVAPG+PFHPFPPLPPYPRGKRG PTSACGT V QS QE QVN 
Sbjct: 1910 IVEQCGTSCAENLECWDVAPGMPFHPFPPLPPYPRGKRGLPTSACGTAVRQSSQEMQVNC 1969

Query: 327  HDSRTSFSEESPPSTSTNYQPDVCTPSNNQQIPKRAKESSYDLGRRYFRQQKWRNSKYGP 386
            HDSRTSFSEE+PPSTSTNYQ D+C  SN QQ  KRAKESSYDLGR+YFRQ+KWRN+K+GP
Sbjct: 1970 HDSRTSFSEETPPSTSTNYQQDLCNLSNIQQTSKRAKESSYDLGRKYFRQEKWRNTKFGP 2029

Query: 387  PWLQRRNQWGCQGNFREGVSTIGDDNIPNEEISPYYSDEASGKVDKANDDFYQHLQNQN 445
            P   R +QWG QGNFR GVST+GDDNIPNE I PY SDEASG+VDKANDDFYQHLQNQN
Sbjct: 2030 P---RTDQWGYQGNFRSGVSTLGDDNIPNEGIRPYCSDEASGRVDKANDDFYQHLQNQN 2084

BLAST of Lsi08G004660 vs. ExPASy TrEMBL
Match: A0A6J1K197 (histone-lysine N-methyltransferase ASHH2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111489728 PE=4 SV=1)

HSP 1 Score: 690.6 bits (1781), Expect = 4.2e-195
Identity = 355/419 (84.73%), Postives = 374/419 (89.26%), Query Frame = 0

Query: 27   QVHQIARSFRDRWFPRHTRKFGYSEREDGRLEGYRGSNCGRFTASHSYRHDQDSRPTDAI 86
            QVHQIARSFRDRWFPRHTRKF YSEREDGRLE YRGSNC RFTASHSYR DQDSRPTDAI
Sbjct: 1665 QVHQIARSFRDRWFPRHTRKFVYSEREDGRLEVYRGSNCSRFTASHSYRRDQDSRPTDAI 1724

Query: 87   DCVKQSM-PSLPDAHPAEVCSVASTAGHSLNGQKVRKRKSRWDQPADTSLDLRSKEQKLE 146
            DCVKQS+  SLPDAHPAEVCS+ASTAGHSLNGQKV KRKSRWDQPADTSLDLRSKEQKLE
Sbjct: 1725 DCVKQSLSTSLPDAHPAEVCSMASTAGHSLNGQKVCKRKSRWDQPADTSLDLRSKEQKLE 1784

Query: 147  STSVQQLNSSQLNDVGVASMLIDKVNNDDKDPSLSDSVGDRCRQDEDIRADSAVQNILED 206
            S SVQQ NSSQL+ VGV SMLIDKVN+DDKD SLSDSVG R  QDEDIRADSAVQNI ED
Sbjct: 1785 SKSVQQFNSSQLSSVGVVSMLIDKVNSDDKDFSLSDSVGVRGSQDEDIRADSAVQNIPED 1844

Query: 207  IPPGFSSPFHPPMASSSTFSTVLDPPRQNIGNLSCAFSTVGHPQERFISRLPVSYGIPFS 266
            IPPGF  PF  P+ASSS FSTVLDPPRQ+IG LSCAFSTVG+PQE+FIS LPVSYGIPFS
Sbjct: 1845 IPPGF-FPFSLPVASSSAFSTVLDPPRQSIGKLSCAFSTVGYPQEKFISCLPVSYGIPFS 1904

Query: 267  IIEQCGTSRAENLECWDVAPGVPFHPFPPLPPYPRGKRGPPTSACGTVVGQSFQEGQVNS 326
            I+EQCGTS AENLECWDVAPG+PFHPFPPLPPYPRGKRG PTSACGT V QS QE QVN 
Sbjct: 1905 IVEQCGTSCAENLECWDVAPGMPFHPFPPLPPYPRGKRGLPTSACGTAVRQSSQEMQVNC 1964

Query: 327  HDSRTSFSEESPPSTSTNYQPDVCTPSNNQQIPKRAKESSYDLGRRYFRQQKWRNSKYGP 386
            HDSRTSFSEE+PPSTSTNYQ D+C  SN QQ  KRAKESSYDLGR+YFRQ+KWRN+K+GP
Sbjct: 1965 HDSRTSFSEETPPSTSTNYQQDLCNLSNIQQTSKRAKESSYDLGRKYFRQEKWRNTKFGP 2024

Query: 387  PWLQRRNQWGCQGNFREGVSTIGDDNIPNEEISPYYSDEASGKVDKANDDFYQHLQNQN 445
            P   R +QWG QGNFR GVST+GDDNIPNE I PY SDEASG+VDKANDDFYQHLQNQN
Sbjct: 2025 P---RTDQWGYQGNFRSGVSTLGDDNIPNEGIRPYCSDEASGRVDKANDDFYQHLQNQN 2079

BLAST of Lsi08G004660 vs. NCBI nr
Match: XP_038885000.1 (histone-lysine N-methyltransferase ASHH2 isoform X2 [Benincasa hispida])

HSP 1 Score: 780.4 bits (2014), Expect = 8.3e-222
Identity = 385/420 (91.67%), Postives = 398/420 (94.76%), Query Frame = 0

Query: 27   QVHQIARSFRDRWFPRHTRKFGYSEREDGRLEGYRGSNCGRFTASHSYRHDQDSRPTDAI 86
            QVHQIARSFRDRWFPRH+RKFGYSEREDGRLE YRGSNC RFTASHSYRHDQDSRPTDAI
Sbjct: 1661 QVHQIARSFRDRWFPRHSRKFGYSEREDGRLEVYRGSNCSRFTASHSYRHDQDSRPTDAI 1720

Query: 87   DCVKQSMP-SLPDAHPAEVCSVASTAGHSLNGQKVRKRKSRWDQPADTSLDLRSKEQKLE 146
            DCVKQSMP SLPDAHP EVCSVASTAGHS NGQKVRKRKSRWDQPADTSLDLRSKEQKLE
Sbjct: 1721 DCVKQSMPTSLPDAHPVEVCSVASTAGHSSNGQKVRKRKSRWDQPADTSLDLRSKEQKLE 1780

Query: 147  STSVQQLNSSQLNDVGVASMLIDKVNNDDKDPSLSDSVGDRCRQDEDIRADSAVQNILED 206
            STSVQQLNSSQLN VG+ASMLIDKVNNDDKD SLSDSVG RCRQDEDIRADSAVQN+ ED
Sbjct: 1781 STSVQQLNSSQLNCVGMASMLIDKVNNDDKDSSLSDSVGVRCRQDEDIRADSAVQNVPED 1840

Query: 207  IPPGFSSPFHPPMASSSTFSTVLDPPRQNIGNLSCAFSTVGHPQERFISRLPVSYGIPFS 266
            IPPGFSSPF+PP+ASSS FSTVLDPPRQNI +L CAFSTVGHPQERFISR+PVSYGIPFS
Sbjct: 1841 IPPGFSSPFNPPVASSSAFSTVLDPPRQNICDLGCAFSTVGHPQERFISRMPVSYGIPFS 1900

Query: 267  IIEQCGTSRAENLECWDVAPGVPFHPFPPLPPYPRGKRGPPTSACGTVVGQSFQEGQVNS 326
            IIEQCGTS AENLECWDVAPGVPFHPFPPLPPYPRGKRGPPTSACGT VGQS QEGQVNS
Sbjct: 1901 IIEQCGTSHAENLECWDVAPGVPFHPFPPLPPYPRGKRGPPTSACGTAVGQSSQEGQVNS 1960

Query: 327  HDSRTSFSEESPPSTSTNYQPDVCTPSNNQQIPKRAKESSYDLGRRYFRQQKWRNSKYGP 386
            HDSRTSFSEESPPSTSTNYQPD+CT SNNQQIP R KESSYDLGRRYFRQQKWRN+KYGP
Sbjct: 1961 HDSRTSFSEESPPSTSTNYQPDLCTSSNNQQIPNRTKESSYDLGRRYFRQQKWRNTKYGP 2020

Query: 387  PWLQRRNQWGCQGNFREGVSTIGDDNIPNEEISPYYSDEASGKVDKANDDFYQHLQNQNL 446
            PWLQ+RNQWGCQGNFR GVS I DDNIPNEEISPY SDEASG++DKAND+FYQHLQNQNL
Sbjct: 2021 PWLQKRNQWGCQGNFRGGVSAIVDDNIPNEEISPYCSDEASGRLDKANDEFYQHLQNQNL 2080

BLAST of Lsi08G004660 vs. NCBI nr
Match: XP_038885001.1 (histone-lysine N-methyltransferase ASHH2 isoform X3 [Benincasa hispida])

HSP 1 Score: 780.4 bits (2014), Expect = 8.3e-222
Identity = 385/420 (91.67%), Postives = 398/420 (94.76%), Query Frame = 0

Query: 27   QVHQIARSFRDRWFPRHTRKFGYSEREDGRLEGYRGSNCGRFTASHSYRHDQDSRPTDAI 86
            QVHQIARSFRDRWFPRH+RKFGYSEREDGRLE YRGSNC RFTASHSYRHDQDSRPTDAI
Sbjct: 1646 QVHQIARSFRDRWFPRHSRKFGYSEREDGRLEVYRGSNCSRFTASHSYRHDQDSRPTDAI 1705

Query: 87   DCVKQSMP-SLPDAHPAEVCSVASTAGHSLNGQKVRKRKSRWDQPADTSLDLRSKEQKLE 146
            DCVKQSMP SLPDAHP EVCSVASTAGHS NGQKVRKRKSRWDQPADTSLDLRSKEQKLE
Sbjct: 1706 DCVKQSMPTSLPDAHPVEVCSVASTAGHSSNGQKVRKRKSRWDQPADTSLDLRSKEQKLE 1765

Query: 147  STSVQQLNSSQLNDVGVASMLIDKVNNDDKDPSLSDSVGDRCRQDEDIRADSAVQNILED 206
            STSVQQLNSSQLN VG+ASMLIDKVNNDDKD SLSDSVG RCRQDEDIRADSAVQN+ ED
Sbjct: 1766 STSVQQLNSSQLNCVGMASMLIDKVNNDDKDSSLSDSVGVRCRQDEDIRADSAVQNVPED 1825

Query: 207  IPPGFSSPFHPPMASSSTFSTVLDPPRQNIGNLSCAFSTVGHPQERFISRLPVSYGIPFS 266
            IPPGFSSPF+PP+ASSS FSTVLDPPRQNI +L CAFSTVGHPQERFISR+PVSYGIPFS
Sbjct: 1826 IPPGFSSPFNPPVASSSAFSTVLDPPRQNICDLGCAFSTVGHPQERFISRMPVSYGIPFS 1885

Query: 267  IIEQCGTSRAENLECWDVAPGVPFHPFPPLPPYPRGKRGPPTSACGTVVGQSFQEGQVNS 326
            IIEQCGTS AENLECWDVAPGVPFHPFPPLPPYPRGKRGPPTSACGT VGQS QEGQVNS
Sbjct: 1886 IIEQCGTSHAENLECWDVAPGVPFHPFPPLPPYPRGKRGPPTSACGTAVGQSSQEGQVNS 1945

Query: 327  HDSRTSFSEESPPSTSTNYQPDVCTPSNNQQIPKRAKESSYDLGRRYFRQQKWRNSKYGP 386
            HDSRTSFSEESPPSTSTNYQPD+CT SNNQQIP R KESSYDLGRRYFRQQKWRN+KYGP
Sbjct: 1946 HDSRTSFSEESPPSTSTNYQPDLCTSSNNQQIPNRTKESSYDLGRRYFRQQKWRNTKYGP 2005

Query: 387  PWLQRRNQWGCQGNFREGVSTIGDDNIPNEEISPYYSDEASGKVDKANDDFYQHLQNQNL 446
            PWLQ+RNQWGCQGNFR GVS I DDNIPNEEISPY SDEASG++DKAND+FYQHLQNQNL
Sbjct: 2006 PWLQKRNQWGCQGNFRGGVSAIVDDNIPNEEISPYCSDEASGRLDKANDEFYQHLQNQNL 2065

BLAST of Lsi08G004660 vs. NCBI nr
Match: XP_038884997.1 (histone-lysine N-methyltransferase ASHH2 isoform X1 [Benincasa hispida] >XP_038884998.1 histone-lysine N-methyltransferase ASHH2 isoform X1 [Benincasa hispida] >XP_038884999.1 histone-lysine N-methyltransferase ASHH2 isoform X1 [Benincasa hispida])

HSP 1 Score: 780.4 bits (2014), Expect = 8.3e-222
Identity = 385/420 (91.67%), Postives = 398/420 (94.76%), Query Frame = 0

Query: 27   QVHQIARSFRDRWFPRHTRKFGYSEREDGRLEGYRGSNCGRFTASHSYRHDQDSRPTDAI 86
            QVHQIARSFRDRWFPRH+RKFGYSEREDGRLE YRGSNC RFTASHSYRHDQDSRPTDAI
Sbjct: 1691 QVHQIARSFRDRWFPRHSRKFGYSEREDGRLEVYRGSNCSRFTASHSYRHDQDSRPTDAI 1750

Query: 87   DCVKQSMP-SLPDAHPAEVCSVASTAGHSLNGQKVRKRKSRWDQPADTSLDLRSKEQKLE 146
            DCVKQSMP SLPDAHP EVCSVASTAGHS NGQKVRKRKSRWDQPADTSLDLRSKEQKLE
Sbjct: 1751 DCVKQSMPTSLPDAHPVEVCSVASTAGHSSNGQKVRKRKSRWDQPADTSLDLRSKEQKLE 1810

Query: 147  STSVQQLNSSQLNDVGVASMLIDKVNNDDKDPSLSDSVGDRCRQDEDIRADSAVQNILED 206
            STSVQQLNSSQLN VG+ASMLIDKVNNDDKD SLSDSVG RCRQDEDIRADSAVQN+ ED
Sbjct: 1811 STSVQQLNSSQLNCVGMASMLIDKVNNDDKDSSLSDSVGVRCRQDEDIRADSAVQNVPED 1870

Query: 207  IPPGFSSPFHPPMASSSTFSTVLDPPRQNIGNLSCAFSTVGHPQERFISRLPVSYGIPFS 266
            IPPGFSSPF+PP+ASSS FSTVLDPPRQNI +L CAFSTVGHPQERFISR+PVSYGIPFS
Sbjct: 1871 IPPGFSSPFNPPVASSSAFSTVLDPPRQNICDLGCAFSTVGHPQERFISRMPVSYGIPFS 1930

Query: 267  IIEQCGTSRAENLECWDVAPGVPFHPFPPLPPYPRGKRGPPTSACGTVVGQSFQEGQVNS 326
            IIEQCGTS AENLECWDVAPGVPFHPFPPLPPYPRGKRGPPTSACGT VGQS QEGQVNS
Sbjct: 1931 IIEQCGTSHAENLECWDVAPGVPFHPFPPLPPYPRGKRGPPTSACGTAVGQSSQEGQVNS 1990

Query: 327  HDSRTSFSEESPPSTSTNYQPDVCTPSNNQQIPKRAKESSYDLGRRYFRQQKWRNSKYGP 386
            HDSRTSFSEESPPSTSTNYQPD+CT SNNQQIP R KESSYDLGRRYFRQQKWRN+KYGP
Sbjct: 1991 HDSRTSFSEESPPSTSTNYQPDLCTSSNNQQIPNRTKESSYDLGRRYFRQQKWRNTKYGP 2050

Query: 387  PWLQRRNQWGCQGNFREGVSTIGDDNIPNEEISPYYSDEASGKVDKANDDFYQHLQNQNL 446
            PWLQ+RNQWGCQGNFR GVS I DDNIPNEEISPY SDEASG++DKAND+FYQHLQNQNL
Sbjct: 2051 PWLQKRNQWGCQGNFRGGVSAIVDDNIPNEEISPYCSDEASGRLDKANDEFYQHLQNQNL 2110

BLAST of Lsi08G004660 vs. NCBI nr
Match: XP_011657417.1 (histone-lysine N-methyltransferase ASHH2 isoform X1 [Cucumis sativus] >XP_011657418.1 histone-lysine N-methyltransferase ASHH2 isoform X1 [Cucumis sativus] >KAE8647263.1 hypothetical protein Csa_018308 [Cucumis sativus])

HSP 1 Score: 749.6 bits (1934), Expect = 1.6e-212
Identity = 376/420 (89.52%), Postives = 391/420 (93.10%), Query Frame = 0

Query: 27   QVHQIARSFRDRWFPRHTRKFGYSEREDGRLEGYRGSNCGRFTASHSYRHDQDSRPTDAI 86
            QVHQIARSFRDRWFPRHTRKFGYSEREDGRLE YRGSN  RFTASHS+RHDQD RPTDAI
Sbjct: 1663 QVHQIARSFRDRWFPRHTRKFGYSEREDGRLEVYRGSNSSRFTASHSFRHDQDCRPTDAI 1722

Query: 87   DCVKQSMP-SLPDAHPAEVCSVASTAGHSLNGQKVRKRKSRWDQPADTSLDLRSKEQKLE 146
            DC+KQSMP SLPDAHPAEVCS+AS A HS+NGQKVRKRKSRWDQPADTSLDLRSKEQKLE
Sbjct: 1723 DCIKQSMPTSLPDAHPAEVCSLASAASHSVNGQKVRKRKSRWDQPADTSLDLRSKEQKLE 1782

Query: 147  STSVQQLNSSQLNDVGVASMLIDKVNNDDKDPSLSDSVGDRCRQDEDIRADSAVQNILED 206
            STSVQ+LNSSQLN VG ASMLIDKVNNDDKD SLSDSVG  CRQDEDIRADSAV NI ED
Sbjct: 1783 STSVQELNSSQLNSVGAASMLIDKVNNDDKDISLSDSVGVPCRQDEDIRADSAVPNIPED 1842

Query: 207  IPPGFSSPFHPPMASSSTFSTVLDPPRQNIGNLSCAFSTVGHPQERFISRLPVSYGIPFS 266
            IPPGFSSPF+PP+ASSS FS VLDPPRQNIG+LSCAFSTVGH QERFISRLPVSYGIPFS
Sbjct: 1843 IPPGFSSPFNPPVASSSAFSAVLDPPRQNIGDLSCAFSTVGHLQERFISRLPVSYGIPFS 1902

Query: 267  IIEQCGTSRAENLECWDVAPGVPFHPFPPLPPYPRGKRGPPTSACGTVVGQSFQEGQVNS 326
            IIEQCGTS AENLECWDVAPGVPFHPFPPLPPYPRG RG PTSACGT  GQS QEGQVNS
Sbjct: 1903 IIEQCGTSHAENLECWDVAPGVPFHPFPPLPPYPRGMRGLPTSACGT-AGQSSQEGQVNS 1962

Query: 327  HDSRTSFSEESPPSTSTNYQPDVCTPSNNQQIPKRAKESSYDLGRRYFRQQKWRNSKYGP 386
            HDSRTSFSEESPPSTSTNYQ D+CTPSNNQQI KRAKESS DLGRRYFRQQKWRN+K+GP
Sbjct: 1963 HDSRTSFSEESPPSTSTNYQTDLCTPSNNQQIAKRAKESSCDLGRRYFRQQKWRNTKFGP 2022

Query: 387  PWLQRRNQWGCQGNFREGVSTIGDDNIPNEEISPYYSDEASGKVDKANDDFYQHLQNQNL 446
            PWLQRR+QWGCQGNFR GVSTIGD+NIP+EEISPY SDEASG+VDKAN DFYQHLQNQNL
Sbjct: 2023 PWLQRRSQWGCQGNFRGGVSTIGDENIPDEEISPYCSDEASGRVDKANGDFYQHLQNQNL 2081

BLAST of Lsi08G004660 vs. NCBI nr
Match: XP_031744047.1 (histone-lysine N-methyltransferase ASHH2 isoform X2 [Cucumis sativus])

HSP 1 Score: 749.6 bits (1934), Expect = 1.6e-212
Identity = 376/420 (89.52%), Postives = 391/420 (93.10%), Query Frame = 0

Query: 27   QVHQIARSFRDRWFPRHTRKFGYSEREDGRLEGYRGSNCGRFTASHSYRHDQDSRPTDAI 86
            QVHQIARSFRDRWFPRHTRKFGYSEREDGRLE YRGSN  RFTASHS+RHDQD RPTDAI
Sbjct: 1626 QVHQIARSFRDRWFPRHTRKFGYSEREDGRLEVYRGSNSSRFTASHSFRHDQDCRPTDAI 1685

Query: 87   DCVKQSMP-SLPDAHPAEVCSVASTAGHSLNGQKVRKRKSRWDQPADTSLDLRSKEQKLE 146
            DC+KQSMP SLPDAHPAEVCS+AS A HS+NGQKVRKRKSRWDQPADTSLDLRSKEQKLE
Sbjct: 1686 DCIKQSMPTSLPDAHPAEVCSLASAASHSVNGQKVRKRKSRWDQPADTSLDLRSKEQKLE 1745

Query: 147  STSVQQLNSSQLNDVGVASMLIDKVNNDDKDPSLSDSVGDRCRQDEDIRADSAVQNILED 206
            STSVQ+LNSSQLN VG ASMLIDKVNNDDKD SLSDSVG  CRQDEDIRADSAV NI ED
Sbjct: 1746 STSVQELNSSQLNSVGAASMLIDKVNNDDKDISLSDSVGVPCRQDEDIRADSAVPNIPED 1805

Query: 207  IPPGFSSPFHPPMASSSTFSTVLDPPRQNIGNLSCAFSTVGHPQERFISRLPVSYGIPFS 266
            IPPGFSSPF+PP+ASSS FS VLDPPRQNIG+LSCAFSTVGH QERFISRLPVSYGIPFS
Sbjct: 1806 IPPGFSSPFNPPVASSSAFSAVLDPPRQNIGDLSCAFSTVGHLQERFISRLPVSYGIPFS 1865

Query: 267  IIEQCGTSRAENLECWDVAPGVPFHPFPPLPPYPRGKRGPPTSACGTVVGQSFQEGQVNS 326
            IIEQCGTS AENLECWDVAPGVPFHPFPPLPPYPRG RG PTSACGT  GQS QEGQVNS
Sbjct: 1866 IIEQCGTSHAENLECWDVAPGVPFHPFPPLPPYPRGMRGLPTSACGT-AGQSSQEGQVNS 1925

Query: 327  HDSRTSFSEESPPSTSTNYQPDVCTPSNNQQIPKRAKESSYDLGRRYFRQQKWRNSKYGP 386
            HDSRTSFSEESPPSTSTNYQ D+CTPSNNQQI KRAKESS DLGRRYFRQQKWRN+K+GP
Sbjct: 1926 HDSRTSFSEESPPSTSTNYQTDLCTPSNNQQIAKRAKESSCDLGRRYFRQQKWRNTKFGP 1985

Query: 387  PWLQRRNQWGCQGNFREGVSTIGDDNIPNEEISPYYSDEASGKVDKANDDFYQHLQNQNL 446
            PWLQRR+QWGCQGNFR GVSTIGD+NIP+EEISPY SDEASG+VDKAN DFYQHLQNQNL
Sbjct: 1986 PWLQRRSQWGCQGNFRGGVSTIGDENIPDEEISPYCSDEASGRVDKANGDFYQHLQNQNL 2044

BLAST of Lsi08G004660 vs. TAIR 10
Match: AT1G77300.1 (histone methyltransferases(H3-K4 specific);histone methyltransferases(H3-K36 specific) )

HSP 1 Score: 141.0 bits (354), Expect = 2.4e-33
Identity = 131/389 (33.68%), Postives = 166/389 (42.67%), Query Frame = 0

Query: 13   KLSKFSFDELSMNLQVHQIARSFRDRWFPRHTRKFGYSEREDGRLEGYRGSNCGRFTASH 72
            K S  SF E   +  VH IARSFRDRW P+H RK     RE+ R E  R     RF AS 
Sbjct: 1502 KDSVLSFTEHD-DYTVHNIARSFRDRWIPKHFRKPWRINREE-RSESMRSPINRRFRASQ 1561

Query: 73   SYRHD-QDSRPTDAIDCVKQSMPSLPDAHPAEVCSVASTAGHSLNGQKVRKRKSRWDQPA 132
              R+D Q  RP +    V  S  + P+   A V    S     L     RKRKSRWDQP+
Sbjct: 1562 EPRYDHQSPRPAEPAASVTSSKAATPET--ASVSEGYSEPNSGLPETNGRKRKSRWDQPS 1621

Query: 133  DTSLDLRSKEQKLESTSVQQLNSSQLNDVGVASMLIDKVNNDDKDPSLSDSVGDRCRQDE 192
             T      KEQ++ +   QQ + +  N                                 
Sbjct: 1622 KT------KEQRIMTILSQQTDETNGN--------------------------------- 1681

Query: 193  DIRADSAVQNILEDIPPGFSSPFHPPMASSSTFSTVLDPPRQNIGNLSCAFSTVGHPQER 252
                    Q++ +D+PPGFSSP               D P           +    PQ++
Sbjct: 1682 --------QDVQDDLPPGFSSP-------------CTDVPD----------AITAQPQQK 1741

Query: 253  FISRLPVSYGIPFSIIEQCGTSRAENLECWDVAPGVPFHPFPPLPPYPRG----KRGPPT 312
            F+SRLPVSYGIP SI+ Q G+   E+   W VAPG+PF+PFPPLPP   G    KR    
Sbjct: 1742 FLSRLPVSYGIPLSIVHQFGSPGKEDPTTWSVAPGMPFYPFPPLPPVSHGEFFAKRN--V 1785

Query: 313  SACGTVVGQSFQEGQVNSHDSRTSFSEESPPSTSTNYQPDVCTPSNNQQIPKRAKE-SSY 372
             AC + +G               ++S E  P+          TP  +   P R +E  S 
Sbjct: 1802 RACSSSMG-------------NLTYSNEILPA----------TPVTDSTAPTRKRELFSS 1785

Query: 373  DLGRRYFRQQKWRNSKYGPPWLQRRNQWG 396
            D+G  YFRQQK    +  PPWL  RN  G
Sbjct: 1862 DIGTTYFRQQK----QSVPPWL--RNNGG 1785

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q2LAE13.3e-3233.68Histone-lysine N-methyltransferase ASHH2 OS=Arabidopsis thaliana OX=3702 GN=ASHH... [more]
Match NameE-valueIdentityDescription
A0A0A0KDR47.6e-21389.52Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G376290 PE=4 SV=1[more]
A0A5A7UPQ61.9e-20888.33Histone-lysine N-methyltransferase ASHH2 OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A1S3B3U91.9e-20888.33LOW QUALITY PROTEIN: histone-lysine N-methyltransferase ASHH2 OS=Cucumis melo OX... [more]
A0A6J1JXF74.2e-19584.73histone-lysine N-methyltransferase ASHH2-like isoform X1 OS=Cucurbita maxima OX=... [more]
A0A6J1K1974.2e-19584.73histone-lysine N-methyltransferase ASHH2-like isoform X2 OS=Cucurbita maxima OX=... [more]
Match NameE-valueIdentityDescription
XP_038885000.18.3e-22291.67histone-lysine N-methyltransferase ASHH2 isoform X2 [Benincasa hispida][more]
XP_038885001.18.3e-22291.67histone-lysine N-methyltransferase ASHH2 isoform X3 [Benincasa hispida][more]
XP_038884997.18.3e-22291.67histone-lysine N-methyltransferase ASHH2 isoform X1 [Benincasa hispida] >XP_0388... [more]
XP_011657417.11.6e-21289.52histone-lysine N-methyltransferase ASHH2 isoform X1 [Cucumis sativus] >XP_011657... [more]
XP_031744047.11.6e-21289.52histone-lysine N-methyltransferase ASHH2 isoform X2 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT1G77300.12.4e-3333.68histone methyltransferases(H3-K4 specific);histone methyltransferases(H3-K36 spe... [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 320..356
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 114..134
NoneNo IPR availablePANTHERPTHR22884:SF496HISTONE-LYSINE N-METHYLTRANSFERASE ASHH2-LIKEcoord: 27..390
NoneNo IPR availablePANTHERPTHR22884SET DOMAIN PROTEINScoord: 27..390

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi08G004660.1Lsi08G004660.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010452 histone H3-K36 methylation
cellular_component GO:0005634 nucleus
molecular_function GO:0046975 histone methyltransferase activity (H3-K36 specific)
molecular_function GO:0008270 zinc ion binding