Lsi08G004290 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi08G004290
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Eukaryotic aspartyl protease family protein
Location	chr08: 11807593 .. 11808894 (+)
RNA-Seq Expression	Lsi08G004290
Synteny	Lsi08G004290

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCAATGGCAAATTTCTGCTTCTTGTTACTGCTTTTGTTGCCAATTCTCTCTTCTGAGCCAGCTTTAAGTGAAAGTTCACTGAAACCTGGGTTTCATTTTGATCTTACACACGTTGATGCTGCCCGAAATTTTACCAAATTCGAAATGCTCCAACGAGGAATCGAGCGTGACAAGACTCGGATGCAAAAATTCAATGAGATGACCATGAACTTTGAAGTTCGAATGCCAGTCGTTGAACAACAAGGGAGCTATCTGATGAACTTGTCACTCGGGACGCCTTCTGTTTCGTTCTCTGCAATTTTCGATACTGGCAGCGAGTTGATTTGGACGCAGTGCAAGCCATGCTTGCAATGCTTCGAGCAGCCAACCCCTGTGTATGATCCTGCACAATCGCCTTCCTTTGCCAATGCAACTCGCGCCGCTGCGTTATGCCAGGCTTTGACCAATTCAAGTTCCAGCAATGGTTGTGAGTATTCAGTTGGTTATGTAGACGGGTCTTTCACCATAGGTTATTTAGCGTTCGAGACCTTGACGCTTGGGGAAGCAAACCAGCAAGTTTCTACACCAAATATAGCTTTCGGGTGCGGGATAAGGAACCAAGTAAAGAGCTTGACGCAGGGTGCTGGCATAGTAGGGTTCAGCCGAGGACCGTTGTCGCTTCTTTCGCAACTCCATATTCAAAAGTTCTCTTACTGTTTAAGTCCAAATGGAACTGGGAGTCTAGCCTTTGGATCATCTTCAACAAGCATTGACAACACAACGAACCGAGCCATCAAGACTACGCCACTGATACAAAATCCATTCGATCCATCTTACTACTATCTATCTCTACAAGGAATTACAGTTGGCCAAAAGTTCTTGCCCGTCCCGTCCTCGTGGTTTAGCCTAAACGCTGATGGCAGTGGCGGCGTGATCATAGATTCTGGCACATCGATCACGTACATAACAGAGGATGCCTTTGATGTGCTGAAGCCAGTTTTTACTTCACAGACAAACCTCCCAGCGATAAACTCAGCAAGTATTGGGCTTGACCTCTGCTTTGAGCTGCCTTCCCACAACAAAAGTAAACTTGATGTGCCTGATTTGATATTTCATTTCGAGGGTCTCGACTTGAAGCTTCCAGTTGAGAACTACATGGTCGTCAATGAGGAGCTAGGGATTGCATGCTTGGCAATGGGAGCTGCAGGAGGTTTGTCGATTTTCGGCAGCATGCAACATCAAAATATGTTGGTTCTTCATGATCTCGAAAAAAAAGTTGTATCATTCATTCCCACGCAATGTTCTCAGCTCAATTACTAG

mRNA sequence

ATGCCAATGGCAAATTTCTGCTTCTTGTTACTGCTTTTGTTGCCAATTCTCTCTTCTGAGCCAGCTTTAAGTGAAAGTTCACTGAAACCTGGGTTTCATTTTGATCTTACACACGTTGATGCTGCCCGAAATTTTACCAAATTCGAAATGCTCCAACGAGGAATCGAGCGTGACAAGACTCGGATGCAAAAATTCAATGAGATGACCATGAACTTTGAAGTTCGAATGCCAGTCGTTGAACAACAAGGGAGCTATCTGATGAACTTGTCACTCGGGACGCCTTCTGTTTCGTTCTCTGCAATTTTCGATACTGGCAGCGAGTTGATTTGGACGCAGTGCAAGCCATGCTTGCAATGCTTCGAGCAGCCAACCCCTGTGTATGATCCTGCACAATCGCCTTCCTTTGCCAATGCAACTCGCGCCGCTGCGTTATGCCAGGCTTTGACCAATTCAAGTTCCAGCAATGGTTGTGAGTATTCAGTTGGTTATGTAGACGGGTCTTTCACCATAGGTTATTTAGCGTTCGAGACCTTGACGCTTGGGGAAGCAAACCAGCAAGTTTCTACACCAAATATAGCTTTCGGGTGCGGGATAAGGAACCAAGTAAAGAGCTTGACGCAGGGTGCTGGCATAGTAGGGTTCAGCCGAGGACCGTTGTCGCTTCTTTCGCAACTCCATATTCAAAAGTTCTCTTACTGTTTAAGTCCAAATGGAACTGGGAGTCTAGCCTTTGGATCATCTTCAACAAGCATTGACAACACAACGAACCGAGCCATCAAGACTACGCCACTGATACAAAATCCATTCGATCCATCTTACTACTATCTATCTCTACAAGGAATTACAGTTGGCCAAAAGTTCTTGCCCGTCCCGTCCTCGTGGTTTAGCCTAAACGCTGATGGCAGTGGCGGCGTGATCATAGATTCTGGCACATCGATCACGTACATAACAGAGGATGCCTTTGATGTGCTGAAGCCAGTTTTTACTTCACAGACAAACCTCCCAGCGATAAACTCAGCAAGTATTGGGCTTGACCTCTGCTTTGAGCTGCCTTCCCACAACAAAAGTAAACTTGATGTGCCTGATTTGATATTTCATTTCGAGGGTCTCGACTTGAAGCTTCCAGTTGAGAACTACATGGTCGTCAATGAGGAGCTAGGGATTGCATGCTTGGCAATGGGAGCTGCAGGAGGTTTGTCGATTTTCGGCAGCATGCAACATCAAAATATGTTGGTTCTTCATGATCTCGAAAAAAAAGTTGTATCATTCATTCCCACGCAATGTTCTCAGCTCAATTACTAG

Coding sequence (CDS)

ATGCCAATGGCAAATTTCTGCTTCTTGTTACTGCTTTTGTTGCCAATTCTCTCTTCTGAGCCAGCTTTAAGTGAAAGTTCACTGAAACCTGGGTTTCATTTTGATCTTACACACGTTGATGCTGCCCGAAATTTTACCAAATTCGAAATGCTCCAACGAGGAATCGAGCGTGACAAGACTCGGATGCAAAAATTCAATGAGATGACCATGAACTTTGAAGTTCGAATGCCAGTCGTTGAACAACAAGGGAGCTATCTGATGAACTTGTCACTCGGGACGCCTTCTGTTTCGTTCTCTGCAATTTTCGATACTGGCAGCGAGTTGATTTGGACGCAGTGCAAGCCATGCTTGCAATGCTTCGAGCAGCCAACCCCTGTGTATGATCCTGCACAATCGCCTTCCTTTGCCAATGCAACTCGCGCCGCTGCGTTATGCCAGGCTTTGACCAATTCAAGTTCCAGCAATGGTTGTGAGTATTCAGTTGGTTATGTAGACGGGTCTTTCACCATAGGTTATTTAGCGTTCGAGACCTTGACGCTTGGGGAAGCAAACCAGCAAGTTTCTACACCAAATATAGCTTTCGGGTGCGGGATAAGGAACCAAGTAAAGAGCTTGACGCAGGGTGCTGGCATAGTAGGGTTCAGCCGAGGACCGTTGTCGCTTCTTTCGCAACTCCATATTCAAAAGTTCTCTTACTGTTTAAGTCCAAATGGAACTGGGAGTCTAGCCTTTGGATCATCTTCAACAAGCATTGACAACACAACGAACCGAGCCATCAAGACTACGCCACTGATACAAAATCCATTCGATCCATCTTACTACTATCTATCTCTACAAGGAATTACAGTTGGCCAAAAGTTCTTGCCCGTCCCGTCCTCGTGGTTTAGCCTAAACGCTGATGGCAGTGGCGGCGTGATCATAGATTCTGGCACATCGATCACGTACATAACAGAGGATGCCTTTGATGTGCTGAAGCCAGTTTTTACTTCACAGACAAACCTCCCAGCGATAAACTCAGCAAGTATTGGGCTTGACCTCTGCTTTGAGCTGCCTTCCCACAACAAAAGTAAACTTGATGTGCCTGATTTGATATTTCATTTCGAGGGTCTCGACTTGAAGCTTCCAGTTGAGAACTACATGGTCGTCAATGAGGAGCTAGGGATTGCATGCTTGGCAATGGGAGCTGCAGGAGGTTTGTCGATTTTCGGCAGCATGCAACATCAAAATATGTTGGTTCTTCATGATCTCGAAAAAAAAGTTGTATCATTCATTCCCACGCAATGTTCTCAGCTCAATTACTAG

Protein sequence

MPMANFCFLLLLLLPILSSEPALSESSLKPGFHFDLTHVDAARNFTKFEMLQRGIERDKTRMQKFNEMTMNFEVRMPVVEQQGSYLMNLSLGTPSVSFSAIFDTGSELIWTQCKPCLQCFEQPTPVYDPAQSPSFANATRAAALCQALTNSSSSNGCEYSVGYVDGSFTIGYLAFETLTLGEANQQVSTPNIAFGCGIRNQVKSLTQGAGIVGFSRGPLSLLSQLHIQKFSYCLSPNGTGSLAFGSSSTSIDNTTNRAIKTTPLIQNPFDPSYYYLSLQGITVGQKFLPVPSSWFSLNADGSGGVIIDSGTSITYITEDAFDVLKPVFTSQTNLPAINSASIGLDLCFELPSHNKSKLDVPDLIFHFEGLDLKLPVENYMVVNEELGIACLAMGAAGGLSIFGSMQHQNMLVLHDLEKKVVSFIPTQCSQLNY

Homology

[Close]

BLAST of Lsi08G004290 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 357.1 bits (915), Expect = 2.9e-97
Identity = 202/444 (45.50%), Postives = 275/444 (61.94%), Query Frame = 0


Query: 1   MPMANFCFLLLLLLPILSSEPALSES---------SLKPGFHFDLTHVDAARNFTKFEML 60
           M  + + FLL L +  +   P  S S         +   GF   L HVD+ +N TKF++L
Sbjct: 1   MASSLYSFLLALSIVYIFVAPTHSTSRTALNHRHEAKVTGFQIMLEHVDSGKNLTKFQLL 60

Query: 61  QRGIERDKTRMQKFNEMTMN-FEVRMPVVEQQGSYLMNLSLGTPSVSFSAIFDTGSELIW 120
           +R IER   R+Q+   M      V   V    G YLMNLS+GTP+  FSAI DTGS+LIW
Sbjct: 61  ERAIERGSRRLQRLEAMLNGPSGVETSVYAGDGEYLMNLSIGTPAQPFSAIMDTGSDLIW 120

Query: 121 TQCKPCLQCFEQPTPVYDPAQSPSFANATRAAALCQALTNSSSSNG-CEYSVGYVDGSFT 180
           TQC+PC QCF Q TP+++P  S SF+    ++ LCQAL++ + SN  C+Y+ GY DGS T
Sbjct: 121 TQCQPCTQCFNQSTPIFNPQGSSSFSTLPCSSQLCQALSSPTCSNNFCQYTYGYGDGSET 180

Query: 181 IGYLAFETLTLGEANQQVSTPNIAFGCGIRNQVKSLTQGAGIVGFSRGPLSLLSQLHIQK 240
            G +  ETLT G     VS PNI FGCG  NQ      GAG+VG  RGPLSL SQL + K
Sbjct: 181 QGSMGTETLTFG----SVSIPNITFGCGENNQGFGQGNGAGLVGMGRGPLSLPSQLDVTK 240

Query: 241 FSYCLSPNGT---GSLAFGSSSTSIDNTTNRAIKTTPLIQNPFDPSYYYLSLQGITVGQK 300
           FSYC++P G+    +L  G    S+ N+       T LIQ+   P++YY++L G++VG  
Sbjct: 241 FSYCMTPIGSSTPSNLLLG----SLANSVTAGSPNTTLIQSSQIPTFYYITLNGLSVGST 300

Query: 301 FLPVPSSWFSLNA-DGSGGVIIDSGTSITYITEDAFDVLKPVFTSQTNLPAINSASIGLD 360
            LP+  S F+LN+ +G+GG+IIDSGT++TY   +A+  ++  F SQ NLP +N +S G D
Sbjct: 301 RLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVNNAYQSVRQEFISQINLPVVNGSSSGFD 360

Query: 361 LCFELPSHNKSKLDVPDLIFHFEGLDLKLPVENYMVVNEELGIACLAMGAAG-GLSIFGS 420
           LCF+ PS + S L +P  + HF+G DL+LP ENY  ++   G+ CLAMG++  G+SIFG+
Sbjct: 361 LCFQTPS-DPSNLQIPTFVMHFDGGDLELPSENYF-ISPSNGLICLAMGSSSQGMSIFGN 420

Query: 421 MQHQNMLVLHDLEKKVVSFIPTQC 429
           +Q QNMLV++D    VVSF   QC
Sbjct: 421 IQQQNMLVVYDTGNSVVSFASAQC 434

[Close]

BLAST of Lsi08G004290 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 7.1e-96
Identity = 186/407 (45.70%), Postives = 265/407 (65.11%), Query Frame = 0


Query: 29  KPGFHFDLTHVDAARNFTKFEMLQRGIERDKTRMQKFNEMTMNFE-VRMPVVEQQGSYLM 88
           +PG   DL  VD+ +N TK+E+++R I+R + RM+  N M  +   +  PV    G YLM
Sbjct: 39  QPGLRVDLEQVDSGKNLTKYELIKRAIKRGERRMRSINAMLQSSSGIETPVYAGDGEYLM 98

Query: 89  NLSLGTPSVSFSAIFDTGSELIWTQCKPCLQCFEQPTPVYDPAQSPSFANATRAAALCQA 148
           N+++GTP  SFSAI DTGS+LIWTQC+PC QCF QPTP+++P  S SF+     +  CQ 
Sbjct: 99  NVAIGTPDSSFSAIMDTGSDLIWTQCEPCTQCFSQPTPIFNPQDSSSFSTLPCESQYCQD 158

Query: 149 L-TNSSSSNGCEYSVGYVDGSFTIGYLAFETLTLGEANQQVSTPNIAFGCGIRNQVKSLT 208
           L + + ++N C+Y+ GY DGS T GY+A ET T     +  S PNIAFGCG  NQ     
Sbjct: 159 LPSETCNNNECQYTYGYGDGSTTQGYMATETFTF----ETSSVPNIAFGCGEDNQGFGQG 218

Query: 209 QGAGIVGFSRGPLSLLSQLHIQKFSYCLSPNGTGS---LAFGSSSTSIDNTTNRAIKTTP 268
            GAG++G   GPLSL SQL + +FSYC++  G+ S   LA GS+++ +   +     +T 
Sbjct: 219 NGAGLIGMGWGPLSLPSQLGVGQFSYCMTSYGSSSPSTLALGSAASGVPEGS----PSTT 278

Query: 269 LIQNPFDPSYYYLSLQGITVGQKFLPVPSSWFSLNADGSGGVIIDSGTSITYITEDAFDV 328
           LI +  +P+YYY++LQGITVG   L +PSS F L  DG+GG+IIDSGT++TY+ +DA++ 
Sbjct: 279 LIHSSLNPTYYYITLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNA 338

Query: 329 LKPVFTSQTNLPAINSASIGLDLCFELPSHNKSKLDVPDLIFHFEGLDLKLPVENYMVVN 388
           +   FT Q NLP ++ +S GL  CF+ PS + S + VP++   F+G  L L  +N ++  
Sbjct: 339 VAQAFTDQINLPTVDESSSGLSTCFQQPS-DGSTVQVPEISMQFDGGVLNLGEQNILISP 398

Query: 389 EELGIACLAMGAAG--GLSIFGSMQHQNMLVLHDLEKKVVSFIPTQC 429
            E G+ CLAMG++   G+SIFG++Q Q   VL+DL+   VSF+PTQC
Sbjct: 399 AE-GVICLAMGSSSQLGISIFGNIQQQETQVLYDLQNLAVSFVPTQC 435

[Close]

BLAST of Lsi08G004290 vs. ExPASy Swiss-Prot
Match: Q6XBF8 (Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 1.6e-60
Identity = 156/451 (34.59%), Postives = 238/451 (52.77%), Query Frame = 0


Query: 3   MANFCFLLLLLLPILSSEPALSESSLKP--GFHFDLTHVDAARN------FTKFEMLQRG 62
           MA+    +LL L +LSS   LS ++ KP  GF  DL H D+ ++       T  + L+  
Sbjct: 1   MASLFSSVLLSLCLLSS-LFLSNANAKPKLGFTADLIHRDSPKSPFYNPMETSSQRLRNA 60

Query: 63  IERDKTRMQKFNEMTMNFEVRMPVVEQQGSYLMNLSLGTPSVSFSAIFDTGSELIWTQCK 122
           I R   R+  F E     + ++ +    G YLMN+S+GTP     AI DTGS+L+WTQC 
Sbjct: 61  IHRSVNRVFHFTEKDNTPQPQIDLTSNSGEYLMNVSIGTPPFPIMAIADTGSDLLWTQCA 120

Query: 123 PCLQCFEQPTPVYDPAQSPSFANATRAAALCQALTN----SSSSNGCEYSVGYVDGSFTI 182
           PC  C+ Q  P++DP  S ++ + + +++ C AL N    S++ N C YS+ Y D S+T 
Sbjct: 121 PCDDCYTQVDPLFDPKTSSTYKDVSCSSSQCTALENQASCSTNDNTCSYSLSYGDNSYTK 180

Query: 183 GYLAFETLTLGEAN-QQVSTPNIAFGCGIRNQVKSLTQGAGIVGFSRGPLSLLSQLHIQ- 242
           G +A +TLTLG ++ + +   NI  GCG  N      +G+GIVG   GP+SL+ QL    
Sbjct: 181 GNIAVDTLTLGSSDTRPMQLKNIIIGCGHNNAGTFNKKGSGIVGLGGGPVSLIKQLGDSI 240

Query: 243 --KFSYCLSP-----NGTGSLAFGSSSTSIDNTTNRAIKTTPLIQNPFDPSYYYLSLQGI 302
             KFSYCL P     + T  + FG+++      +   + +TPLI      ++YYL+L+ I
Sbjct: 241 DGKFSYCLVPLTSKKDQTSKINFGTNAI----VSGSGVVSTPLIAKASQETFYYLTLKSI 300

Query: 303 TVGQKFLPVPSSWFSLNADGSGGVIIDSGTSITYITEDAFDVLKPVFTSQTNLPAINSAS 362
           +VG K +       S +    G +IIDSGT++T +  + +  L+    S  +        
Sbjct: 301 SVGSKQIQYSG---SDSESSEGNIIIDSGTTLTLLPTEFYSELEDAVASSIDAEKKQDPQ 360

Query: 363 IGLDLCFELPSHNKSKLDVPDLIFHFEGLDLKLPVEN-YMVVNEELGIACLAMGAAGGLS 422
            GL LC+         L VP +  HF+G D+KL   N ++ V+E+L   C A   +   S
Sbjct: 361 SGLSLCYSA----TGDLKVPVITMHFDGADVKLDSSNAFVQVSEDL--VCFAFRGSPSFS 420

Query: 423 IFGSMQHQNMLVLHDLEKKVVSFIPTQCSQL 432
           I+G++   N LV +D   K VSF PT C+++
Sbjct: 421 IYGNVAQMNFLVGYDTVSKTVSFKPTDCAKM 437

[Close]

BLAST of Lsi08G004290 vs. ExPASy Swiss-Prot
Match: Q7XV21 (Aspartyl protease 37 OS=Oryza sativa subsp. japonica OX=39947 GN=AP37 PE=3 SV=2)

HSP 1 Score: 218.0 bits (554), Expect = 2.1e-55
Identity = 153/468 (32.69%), Postives = 232/468 (49.57%), Query Frame = 0


Query: 10  LLLLLPILSSEPALSESSLKP-GFHFDLTHVDA----ARNFTKFEMLQRGIERDKTRMQK 69
           +LLLL  L++ PA   S   P  F  +L  VDA    A N T+ E+L+R I+R + R+  
Sbjct: 5   VLLLLLALAALPA---SCAPPRSFRLELASVDASAADAANLTEHELLRRAIQRSRYRLAG 64

Query: 70  FN-------EMTMNFEVRMPVVEQQGSYLMNLSLGTPSVSFSAIFDTGSELIWTQCKPCL 129
                              P++   G YL+ L +GTP   F+A  DT S+LIWTQC+PC 
Sbjct: 65  IGMARGEAASARKAVVAETPIMPAGGEYLVKLGIGTPPYKFTAAIDTASDLIWTQCQPCT 124

Query: 130 QCFEQPTPVYDPAQSPSFANATRAAALCQAL----TNSSSSNGCEYSVGYVDGSFTIGYL 189
            C+ Q  P+++P  S ++A    ++  C  L            C+Y+  Y   + T G L
Sbjct: 125 GCYHQVDPMFNPRVSSTYAALPCSSDTCDELDVHRCGHDDDESCQYTYTYSGNATTEGTL 184

Query: 190 AFETLTLGEANQQVSTPNIAFGCGIRNQVKS-LTQGAGIVGFSRGPLSLLSQLHIQKFSY 249
           A + L +GE     +   +AFGC   +   +   Q +G+VG  RGPLSL+SQL +++F+Y
Sbjct: 185 AVDKLVIGED----AFRGVAFGCSTSSTGGAPPPQASGVVGLGRGPLSLVSQLSVRRFAY 244

Query: 250 CLSPNGT---GSLAFGSSSTSIDNTTNRAIKTTPLIQNPFDPSYYYLSLQGITVGQKFL- 309
           CL P  +   G L  G+ + +  N TNR     P+ ++P  PSYYYL+L G+ +G + + 
Sbjct: 245 CLPPPASRIPGKLVLGADADAARNATNRI--AVPMRRDPRYPSYYYLNLDGLLIGDRAMS 304

Query: 310 ---------------PVPSSWFSLNADGSG-------GVIIDSGTSITYITEDAFDVLKP 369
                          P P+   S NA           G+IID  ++IT++    +D L  
Sbjct: 305 LPPTTTTTATATATAPAPAPTPSPNATAVAVGDANRYGMIIDIASTITFLEASLYDELVN 364

Query: 370 VFTSQTNLPAINSASIGLDLCFELPSHNK-SKLDVPDLIFHFEGLDLKLPVENYMVVNEE 429
               +  LP    +S+GLDLCF LP      ++ VP +   F+G  L+L        + E
Sbjct: 365 DLEVEIRLPRGTGSSLGLDLCFILPDGVAFDRVYVPAVALAFDGRWLRLDKARLFAEDRE 424

Query: 430 LGIACLAMG--AAGGLSIFGSMQHQNMLVLHDLEKKVVSFIPTQCSQL 432
            G+ CL +G   AG +SI G+ Q QNM VL++L +  V+F+ + C  L
Sbjct: 425 SGMMCLMVGRAEAGSVSILGNFQQQNMQVLYNLRRGRVTFVQSPCGAL 463

[Close]

BLAST of Lsi08G004290 vs. ExPASy Swiss-Prot
Match: Q3EBM5 (Probable aspartic protease At2g35615 OS=Arabidopsis thaliana OX=3702 GN=At2g35615 PE=3 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 2.1e-55
Identity = 146/447 (32.66%), Postives = 238/447 (53.24%), Query Frame = 0


Query: 11  LLLLPILSSEPALSESSLKPGFHFDLTHVDAARN------FTKFEMLQRGIERDKTRMQK 70
           +LL   L     LS S     F  +L H D+  +       T  + L     R  +R ++
Sbjct: 5   ILLCFFLFFSVTLSSSGHPKNFSVELIHRDSPLSPIYNPQITVTDRLNAAFLRSVSRSRR 64

Query: 71  FNEMTMNFEVRMPVVEQQGSYLMNLSLGTPSVSFSAIFDTGSELIWTQCKPCLQCFEQPT 130
           FN      +++  ++   G + M++++GTP +   AI DTGS+L W QCKPC QC+++  
Sbjct: 65  FNHQLSQTDLQSGLIGADGEFFMSITIGTPPIKVFAIADTGSDLTWVQCKPCQQCYKENG 124

Query: 131 PVYDPAQSPSFANATRAAALCQALTNS-----SSSNGCEYSVGYVDGSFTIGYLAFETLT 190
           P++D  +S ++ +    +  CQAL+++      S+N C+Y   Y D SF+ G +A ET++
Sbjct: 125 PIFDKKKSSTYKSEPCDSRNCQALSSTERGCDESNNICKYRYSYGDQSFSKGDVATETVS 184

Query: 191 LGEAN-QQVSTPNIAFGCGIRNQVKSLTQGAGIVGFSRGPLSLLSQLH---IQKFSYCLS 250
           +  A+   VS P   FGCG  N       G+GI+G   G LSL+SQL     +KFSYCLS
Sbjct: 185 IDSASGSPVSFPGTVFGCGYNNGGTFDETGSGIIGLGGGHLSLISQLGSSISKKFSYCLS 244

Query: 251 -----PNGTGSLAFGSSSTSIDNTTNRAIKTTPLIQNPFDP-SYYYLSLQGITVGQKFLP 310
                 NGT  +  G++S     + +  + +TPL+    +P +YYYL+L+ I+VG+K +P
Sbjct: 245 HKSATTNGTSVINLGTNSIPSSLSKDSGVVSTPLVDK--EPLTYYYLTLEAISVGKKKIP 304

Query: 311 VPSSWFSLNADG-----SGGVIIDSGTSITYITEDAFDVL-KPVFTSQTNLPAINSASIG 370
              S ++ N DG     SG +IIDSGT++T +    FD     V  S T    ++     
Sbjct: 305 YTGSSYNPNDDGILSETSGNIIIDSGTTLTLLEAGFFDKFSSAVEESVTGAKRVSDPQGL 364

Query: 371 LDLCFELPSHNKSKLDVPDLIFHFEGLDLKL-PVENYMVVNEELGIACLAMGAAGGLSIF 430
           L  CF+  S   +++ +P++  HF G D++L P+  ++ ++E++   CL+M     ++I+
Sbjct: 365 LSHCFKSGS---AEIGLPEITVHFTGADVRLSPINAFVKLSEDM--VCLSMVPTTEVAIY 424

[Close]

BLAST of Lsi08G004290 vs. ExPASy TrEMBL
Match: A0A2N9HF56 (Peptidase A1 domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS40859 PE=3 SV=1)

HSP 1 Score: 458.8 bits (1179), Expect = 2.6e-125
Identity = 244/439 (55.58%), Postives = 311/439 (70.84%), Query Frame = 0


Query: 6   FCFLLLLLLPIL-----SSEPALSESSLKPGFHFDLTHVDAA-RNFTKFEMLQRG-IERD 65
           F F LLLL+ IL     SS     ES  K GF   LT VDA  +N T+ +++QR  I R 
Sbjct: 8   FQFSLLLLVVILSLLSFSSGLPFDESPEKTGFRVSLTRVDAGKKNLTELQLVQRAIINRV 67

Query: 66  KTRMQKFNEMTMNF---EVRMPVVEQQGSYLMNLSLGTPSVSFSAIFDTGSELIWTQCKP 125
           KTR+ + + +T N    +++ P+    G +LM+LS+GTP+V F AI DTGS+LIWTQCKP
Sbjct: 68  KTRLDRLSAITKNAPNDDIQAPIYVGDGEFLMHLSIGTPAVPFLAIMDTGSDLIWTQCKP 127

Query: 126 CLQCFEQPTPVYDPAQSPSFANATRAAALCQALTNSSSSNGCEYSVGYVDGSFTIGYLAF 185
           C  CF Q TPV++PA S SF+N T ++ LC+AL+NS   NGCEY   Y D S + G+LA 
Sbjct: 128 CSNCFNQSTPVFEPALSSSFSNVTCSSELCKALSNSRCGNGCEYLNRYGDESSSEGFLAM 187

Query: 186 ETLTLGEANQQVSTPNIAFGCGIRNQVKSLTQGAGIVGFSRGPLSLLSQLHIQKFSYCLS 245
           ETLT GE+ Q+VS PNI FGCG+ N+ K   + AG+VG  RGPLSL+SQL  +KFSYCL+
Sbjct: 188 ETLTFGESAQRVSVPNIGFGCGVDNKGKGWNKAAGLVGLGRGPLSLVSQLGSKKFSYCLT 247

Query: 246 ---PNGTGSLAFGSSSTSIDNTTNRAIKTTPLIQNPFDPSYYYLSLQGITVGQKFLPVPS 305
               N + SL FGS +  ++ +    +KTTPLI+NP  PS+YYLSL+GITVGQ  LP+P 
Sbjct: 248 SIGDNKSSSLLFGSLA-DLNLSNGAVLKTTPLIRNPILPSFYYLSLEGITVGQTLLPIPK 307

Query: 306 SWFSLNADGSGGVIIDSGTSITYITEDAFDVLKPVFTSQTNLPAINSASIGLDLCFELPS 365
           S F L +DG GGVI+DSGT+IT + EDAFDVLK VF +QT L   N  + GLD+CF+LPS
Sbjct: 308 SLFQLGSDGGGGVILDSGTTITSLHEDAFDVLKQVFKAQTKLRVSNLYTRGLDVCFDLPS 367

Query: 366 HNKSKLDVPDLIFHFEGLDLKLPVENYMVVNEELGIACLAMGAAGGLSIFGSMQHQNMLV 425
            +   + VP L+FHFEGLDL+LPVENYM+ + +LG+ CLAMGA G LSIFG++Q QNMLV
Sbjct: 368 QDVKDIKVPKLVFHFEGLDLELPVENYMIADTKLGVVCLAMGATGRLSIFGNIQQQNMLV 427

Query: 426 LHDLEKKVVSFIPTQCSQL 432
           LHDLEK+ VSFIPT+C QL
Sbjct: 428 LHDLEKETVSFIPTKCDQL 445

[Close]

BLAST of Lsi08G004290 vs. ExPASy TrEMBL
Match: A0A4U5QLX0 (Aspartyl protease family protein OS=Populus alba OX=43335 GN=D5086_0000085760 PE=3 SV=1)

HSP 1 Score: 441.0 bits (1133), Expect = 5.6e-120
Identity = 220/419 (52.51%), Postives = 294/419 (70.17%), Query Frame = 0


Query: 18  SSEPALSESSLKPGFHFDLTHVDAARNFTKFEMLQRGIERDKTRMQKFNEMTM----NFE 77
           +S   L    ++ GF   L HVD+ +N TKFE +Q G++R + R+Q+F  M +    N E
Sbjct: 26  TSRRVLEHPKVQNGFRVKLKHVDSGKNLTKFERIQHGVKRGRHRLQRFKAMALVASSNSE 85

Query: 78  VRMPVVEQQGSYLMNLSLGTPSVSFSAIFDTGSELIWTQCKPCLQCFEQPTPVYDPAQSP 137
           +  PV+   G +LM L++GTP  ++SAI DTGS+LIWTQCKPC QCF+QPTP++DP +S 
Sbjct: 86  IDAPVLPGNGEFLMKLAIGTPPETYSAIMDTGSDLIWTQCKPCTQCFDQPTPIFDPKKSS 145

Query: 138 SFANATRAAALCQALTNSSSSNGCEYSVGYVDGSFTIGYLAFETLTLGEANQQVSTPNIA 197
           SF+  + ++ LC+AL  S+ S+GCEY  GY D S T G LA ETLT G    +VS P +A
Sbjct: 146 SFSKLSCSSKLCEALPQSTCSDGCEYLYGYGDYSSTQGILASETLTFG----KVSVPKVA 205

Query: 198 FGCGIRNQVKSLTQGAGIVGFSRGPLSLLSQLHIQKFSYCL-SPNGTGSLAFGSSSTSID 257
           FGCG  N+    +QG+G+VG  RGPLSL+SQL   KFSYCL S + T +      S +  
Sbjct: 206 FGCGEDNEGSGFSQGSGLVGLGRGPLSLVSQLEEPKFSYCLTSVDDTKASTLLMGSLASV 265

Query: 258 NTTNRAIKTTPLIQNPFDPSYYYLSLQGITVGQKFLPVPSSWFSLNADGSGGVIIDSGTS 317
             ++  IKTTPLIQN   PS+YYLSL+GI+VG   LP+  S FSL  DGSGG+IIDSGT+
Sbjct: 266 KASDSEIKTTPLIQNSAQPSFYYLSLEGISVGDTSLPIKKSTFSLQEDGSGGLIIDSGTT 325

Query: 318 ITYITEDAFDVLKPVFTSQTNLPAINSASIGLDLCFELPSHNKSKLDVPDLIFHFEGLDL 377
           ITY+ + AFD++   FTSQ NLP  NS S GL++CF LPS   + ++VP L+FHF+G DL
Sbjct: 326 ITYLEQSAFDLVAKEFTSQINLPVDNSGSTGLEVCFTLPS-GSTDIEVPKLVFHFDGADL 385

Query: 378 KLPVENYMVVNEELGIACLAMGAAGGLSIFGSMQHQNMLVLHDLEKKVVSFIPTQCSQL 432
           +LP ENYM+ +  +G+ACLAMG++ G+SIFG++Q QNMLVLHDLEK+ +SF+PTQC +L
Sbjct: 386 ELPAENYMIADASMGVACLAMGSSSGMSIFGNIQQQNMLVLHDLEKETLSFLPTQCDEL 439

[Close]

BLAST of Lsi08G004290 vs. ExPASy TrEMBL
Match: A0A4U5R6H0 (Aspartyl protease family protein OS=Populus alba OX=43335 GN=D5086_0000004600 PE=3 SV=1)

HSP 1 Score: 440.7 bits (1132), Expect = 7.3e-120
Identity = 220/419 (52.51%), Postives = 294/419 (70.17%), Query Frame = 0


Query: 18  SSEPALSESSLKPGFHFDLTHVDAARNFTKFEMLQRGIERDKTRMQKFNEMTM----NFE 77
           +S   L    ++ GF   L HVD+ +N TKFE +Q G++R + R+Q+F  M +    N E
Sbjct: 26  TSRRVLEHPKVRNGFRVKLKHVDSGKNLTKFERIQHGVKRGRHRLQRFKAMALVASSNSE 85

Query: 78  VRMPVVEQQGSYLMNLSLGTPSVSFSAIFDTGSELIWTQCKPCLQCFEQPTPVYDPAQSP 137
           +  PV+   G +LM L++GTP  ++SAI DTGS+LIWTQCKPC QCF+QPTP++DP +S 
Sbjct: 86  IDAPVLPGNGEFLMKLAIGTPPETYSAIMDTGSDLIWTQCKPCTQCFDQPTPIFDPKKSS 145

Query: 138 SFANATRAAALCQALTNSSSSNGCEYSVGYVDGSFTIGYLAFETLTLGEANQQVSTPNIA 197
           SF+  + ++ LC+AL  S+ S+GCEY  GY D S T G LA ETLT G    +VS P +A
Sbjct: 146 SFSKLSCSSKLCEALPQSTCSDGCEYLYGYGDYSSTQGILASETLTFG----KVSVPKVA 205

Query: 198 FGCGIRNQVKSLTQGAGIVGFSRGPLSLLSQLHIQKFSYCL-SPNGTGSLAFGSSSTSID 257
           FGCG  N+    +QG+G+VG  RGPLSL+SQL   KFSYCL S + T +      S +  
Sbjct: 206 FGCGEDNEGSGFSQGSGLVGLGRGPLSLVSQLEEPKFSYCLTSVDDTKASTLLMGSLASV 265

Query: 258 NTTNRAIKTTPLIQNPFDPSYYYLSLQGITVGQKFLPVPSSWFSLNADGSGGVIIDSGTS 317
             ++  IKTTPLIQN   PS+YYLSL+GI+VG   LP+  S FSL  DGSGG+IIDSGT+
Sbjct: 266 KASDSEIKTTPLIQNSAQPSFYYLSLEGISVGDTSLPIKKSTFSLQEDGSGGLIIDSGTT 325

Query: 318 ITYITEDAFDVLKPVFTSQTNLPAINSASIGLDLCFELPSHNKSKLDVPDLIFHFEGLDL 377
           ITY+ + AFD++   FTSQ NLP  NS S GL++CF LPS   + ++VP L+FHF+G DL
Sbjct: 326 ITYLEQSAFDLVAKEFTSQINLPVDNSGSTGLEVCFTLPS-GSTDIEVPKLVFHFDGADL 385

Query: 378 KLPVENYMVVNEELGIACLAMGAAGGLSIFGSMQHQNMLVLHDLEKKVVSFIPTQCSQL 432
           +LP ENYM+ +  +G+ACLAMG++ G+SIFG++Q QNMLVLHDLEK+ +SF+PTQC +L
Sbjct: 386 ELPPENYMIADASMGVACLAMGSSSGMSIFGNIQQQNMLVLHDLEKETLSFLPTQCDEL 439

[Close]

BLAST of Lsi08G004290 vs. ExPASy TrEMBL
Match: B9N2J4 (Peptidase A1 domain-containing protein OS=Populus trichocarpa OX=3694 GN=POPTR_019G002100 PE=3 SV=1)

HSP 1 Score: 440.3 bits (1131), Expect = 9.5e-120
Identity = 220/419 (52.51%), Postives = 294/419 (70.17%), Query Frame = 0


Query: 18  SSEPALSESSLKPGFHFDLTHVDAARNFTKFEMLQRGIERDKTRMQKFNEMTM----NFE 77
           +S   L    ++ GF   L HVD+ +N TKFE +Q G++R + R+Q+F  M +    N E
Sbjct: 26  TSRRVLEHPKVQNGFRAKLKHVDSGKNLTKFERIQHGVKRGRHRLQRFKAMALVASSNSE 85

Query: 78  VRMPVVEQQGSYLMNLSLGTPSVSFSAIFDTGSELIWTQCKPCLQCFEQPTPVYDPAQSP 137
           +  PV+   G +LM L++GTP  ++SAI DTGS+LIWTQCKPC QCF+QPTP++DP +S 
Sbjct: 86  IDAPVLPGNGEFLMKLAIGTPPETYSAIMDTGSDLIWTQCKPCTQCFDQPTPIFDPKKSS 145

Query: 138 SFANATRAAALCQALTNSSSSNGCEYSVGYVDGSFTIGYLAFETLTLGEANQQVSTPNIA 197
           SF+  + ++ LC+AL  S+ S+GCEY  GY D S T G LA ETLT G    +VS P +A
Sbjct: 146 SFSKLSCSSKLCEALPQSTCSDGCEYLYGYGDYSSTQGMLASETLTFG----KVSVPEVA 205

Query: 198 FGCGIRNQVKSLTQGAGIVGFSRGPLSLLSQLHIQKFSYCL-SPNGTGSLAFGSSSTSID 257
           FGCG  N+    +QG+G+VG  RGPLSL+SQL   KFSYCL S + T +      S +  
Sbjct: 206 FGCGEDNEGSGFSQGSGLVGLGRGPLSLVSQLKEPKFSYCLTSVDDTKASTLLMGSLASV 265

Query: 258 NTTNRAIKTTPLIQNPFDPSYYYLSLQGITVGQKFLPVPSSWFSLNADGSGGVIIDSGTS 317
             ++  IKTTPLIQN   PS+YYLSL+GI+VG   LP+  S FSL  DGSGG+IIDSGT+
Sbjct: 266 KASDSEIKTTPLIQNSAQPSFYYLSLEGISVGDTSLPIKKSTFSLQEDGSGGLIIDSGTT 325

Query: 318 ITYITEDAFDVLKPVFTSQTNLPAINSASIGLDLCFELPSHNKSKLDVPDLIFHFEGLDL 377
           ITY+ + AFD++   FTSQ NLP  NS S GL++CF LPS   + ++VP L+FHF+G DL
Sbjct: 326 ITYLEQSAFDLVAKEFTSQINLPVDNSGSTGLEVCFTLPS-GSTDIEVPKLVFHFDGADL 385

Query: 378 KLPVENYMVVNEELGIACLAMGAAGGLSIFGSMQHQNMLVLHDLEKKVVSFIPTQCSQL 432
           +LP ENYM+ +  +G+ACLAMG++ G+SIFG++Q QNMLVLHDLEK+ +SF+PTQC +L
Sbjct: 386 ELPAENYMIADASMGVACLAMGSSSGMSIFGNIQQQNMLVLHDLEKETLSFLPTQCDEL 439

[Close]

BLAST of Lsi08G004290 vs. ExPASy TrEMBL
Match: A0A6M2F5X8 (Peptidase A1 domain-containing protein OS=Populus davidiana OX=266767 PE=3 SV=1)

HSP 1 Score: 439.1 bits (1128), Expect = 2.1e-119
Identity = 220/419 (52.51%), Postives = 294/419 (70.17%), Query Frame = 0


Query: 18  SSEPALSESSLKPGFHFDLTHVDAARNFTKFEMLQRGIERDKTRMQKFNEMTM----NFE 77
           +S   L    ++ GF   L HVD+ +N TKFE +Q G++R + R+Q+F  M +    N E
Sbjct: 23  TSRRVLEHPKVRNGFRVKLKHVDSGKNLTKFERIQHGVKRGRHRLQRFKAMALVASSNSE 82

Query: 78  VRMPVVEQQGSYLMNLSLGTPSVSFSAIFDTGSELIWTQCKPCLQCFEQPTPVYDPAQSP 137
           +  PV+   G +LM L++GTP  ++SAI DTGS+LIWTQCKPC QCF+QPTP++DP +S 
Sbjct: 83  IDAPVLPGNGEFLMKLAIGTPPETYSAIMDTGSDLIWTQCKPCTQCFDQPTPIFDPKKSS 142

Query: 138 SFANATRAAALCQALTNSSSSNGCEYSVGYVDGSFTIGYLAFETLTLGEANQQVSTPNIA 197
           SF+  + ++ LC+AL  S+ S+GCEY  GY D S T G LA ETLT G    +VS P +A
Sbjct: 143 SFSKLSCSSKLCEALPQSTCSDGCEYLYGYGDYSSTQGILASETLTFG----KVSVPKVA 202

Query: 198 FGCGIRNQVKSLTQGAGIVGFSRGPLSLLSQLHIQKFSYCL-SPNGTGSLAFGSSSTSID 257
           FGCG  N+    +QG+G+VG  RGPLSL+SQL   KFSYCL S + T +      S +  
Sbjct: 203 FGCGEDNEGSGFSQGSGLVGLGRGPLSLVSQLDEPKFSYCLTSVDDTKASTLLMGSLASV 262

Query: 258 NTTNRAIKTTPLIQNPFDPSYYYLSLQGITVGQKFLPVPSSWFSLNADGSGGVIIDSGTS 317
             ++  IKTTPLIQN   PS+YYLSL+GI+VG   LP+  S FSL  DGSGG+IIDSGT+
Sbjct: 263 KASDSEIKTTPLIQNSAQPSFYYLSLEGISVGATRLPIKKSTFSLQDDGSGGLIIDSGTT 322

Query: 318 ITYITEDAFDVLKPVFTSQTNLPAINSASIGLDLCFELPSHNKSKLDVPDLIFHFEGLDL 377
           ITY+ + AFD++   FTSQ NLP  +S S GLD+CF LPS   + ++VP L+FHF+G DL
Sbjct: 323 ITYLEQSAFDLVAKEFTSQINLPVDSSGSTGLDVCFTLPS-GSTNIEVPKLVFHFDGADL 382

Query: 378 KLPVENYMVVNEELGIACLAMGAAGGLSIFGSMQHQNMLVLHDLEKKVVSFIPTQCSQL 432
           +LP ENYM+ +  +G+ACLAMG++ G+SIFG++Q QNMLVLHDLEK+ +SF+PTQC +L
Sbjct: 383 ELPAENYMIGDSSMGVACLAMGSSSGMSIFGNIQQQNMLVLHDLEKETLSFLPTQCDEL 436

[Close]

BLAST of Lsi08G004290 vs. NCBI nr
Match: XP_038886455.1 (aspartic proteinase nepenthesin-2-like [Benincasa hispida])

HSP 1 Score: 735.3 bits (1897), Expect = 3.0e-208
Identity = 371/434 (85.48%), Postives = 400/434 (92.17%), Query Frame = 0


Query: 1   MPMANFCFLLLLLLPILSSEPALSESSLKPGFHFDLTHVDAARNFTKFEMLQRGIERDKT 60
           MPMANFCFLLLLLLP+LSS+PA SESSLKPGFHFDLTHVDA  NF+KFE+L+RGI+RDKT
Sbjct: 1   MPMANFCFLLLLLLPLLSSKPAFSESSLKPGFHFDLTHVDATWNFSKFEILRRGIKRDKT 60

Query: 61  RMQKFNEMTM-NFEVRMPVVEQQGSYLMNLSLGTPSVSFSAIFDTGSELIWTQCKPCLQC 120
            +Q FN+M + N +VRMPVVEQ+GSYLMNLSLGTP +SFSAIFDTGS+L+WTQCKPCLQC
Sbjct: 61  WIQNFNKMIISNLKVRMPVVEQRGSYLMNLSLGTPPISFSAIFDTGSDLVWTQCKPCLQC 120

Query: 121 FEQPTPVYDPAQSPSFANATRAAALCQALTNSSSSNGCEYSVGYVDGSFTIGYLAFETLT 180
           F+QPTPVYDPAQS SFANATR+AALC+ LTNSSSSNGCEYS  Y D SFT GYLAFETLT
Sbjct: 121 FKQPTPVYDPAQSSSFANATRSAALCKELTNSSSSNGCEYSFSYADESFTTGYLAFETLT 180

Query: 181 LGEANQQVSTPNIAFGCGIRNQVKSLTQGAGIVGFSRGPLSLLSQLHIQKFSYCLSPNGT 240
           LG ANQ+VSTP+IAFGCGI+N VK LTQGAGIVGF+RGPLSLLSQLHI+KFSYCLS NGT
Sbjct: 181 LGGANQKVSTPDIAFGCGIKNHVKGLTQGAGIVGFNRGPLSLLSQLHIKKFSYCLSLNGT 240

Query: 241 GSLAFGSSSTSIDNTTNRAIKTTPLIQNPFDPSYYYLSLQGITVGQKFLPVPSSWFSLNA 300
           GSL FG SSTSIDNTTNRAIKTTPLIQNP++PSYYYLSLQGITVGQK+LPVPSS F LN 
Sbjct: 241 GSLVFG-SSTSIDNTTNRAIKTTPLIQNPYNPSYYYLSLQGITVGQKYLPVPSSSFRLNT 300

Query: 301 DGSGGVIIDSGTSITYITEDAFDVLKPVFTSQTNLPAINSASIGLDLCFELPSHNKSKLD 360
           DGSGG+IIDSGTS+TYITED FDVLKPVF SQTNL  I S SIGLDLCF+LPSHN SKLD
Sbjct: 301 DGSGGMIIDSGTSMTYITEDVFDVLKPVFASQTNLQVIWS-SIGLDLCFQLPSHNNSKLD 360

Query: 361 VPDLIFHFEGLDLKLPVENYMVVNEELGIACLAMGAAGGLSIFGSMQHQNMLVLHDLEKK 420
           VPDLIFHFE LDLKLPVENYMVVNEELGI CLAMGAAGGLSIFGSMQHQNMLVLHDLEKK
Sbjct: 361 VPDLIFHFEDLDLKLPVENYMVVNEELGIVCLAMGAAGGLSIFGSMQHQNMLVLHDLEKK 420

Query: 421 VVSFIPTQCSQLNY 434
           VVSFIPTQCSQL+Y
Sbjct: 421 VVSFIPTQCSQLDY 432

[Close]

BLAST of Lsi08G004290 vs. NCBI nr
Match: KAF9660916.1 (hypothetical protein SADUNF_Sadunf19G0013400 [Salix dunnii])

HSP 1 Score: 441.4 bits (1134), Expect = 8.8e-120
Identity = 221/419 (52.74%), Postives = 293/419 (69.93%), Query Frame = 0


Query: 18  SSEPALSESSLKPGFHFDLTHVDAARNFTKFEMLQRGIERDKTRMQKFNEMTM----NFE 77
           +S   L    ++ GF   L HVD+ +N TKFE +Q G++R + R+Q+F  M +    N E
Sbjct: 283 TSRRVLEHPKVQNGFRVKLKHVDSGKNLTKFERIQHGVKRGRHRLQRFKAMALVASSNSE 342

Query: 78  VRMPVVEQQGSYLMNLSLGTPSVSFSAIFDTGSELIWTQCKPCLQCFEQPTPVYDPAQSP 137
           V  PV    G +LM L++GTP  ++SAI DTGS+LIWTQCKPC QCF+QPTP++DP +S 
Sbjct: 343 VDAPVFPGNGEFLMKLAIGTPPETYSAILDTGSDLIWTQCKPCTQCFDQPTPIFDPKKSS 402

Query: 138 SFANATRAAALCQALTNSSSSNGCEYSVGYVDGSFTIGYLAFETLTLGEANQQVSTPNIA 197
           SF+  + ++ LC+AL  S+ S+GCEY  GY D S T G +A ETLT G    +VS PN+A
Sbjct: 403 SFSKLSCSSKLCEALPQSTCSDGCEYLYGYGDYSSTQGVMASETLTFG----KVSVPNVA 462

Query: 198 FGCGIRNQVKSLTQGAGIVGFSRGPLSLLSQLHIQKFSYCLSP-NGTGSLAFGSSSTSID 257
            GCG  N+    +QG+G+VG  RGPLSL+SQL   KFSYCL+  + T S      S +  
Sbjct: 463 LGCGEDNEGSGFSQGSGLVGLGRGPLSLVSQLKEPKFSYCLTAVDDTKSSTLLMGSLASV 522

Query: 258 NTTNRAIKTTPLIQNPFDPSYYYLSLQGITVGQKFLPVPSSWFSLNADGSGGVIIDSGTS 317
             +   IKTTPLIQN   PS+YYLSL+GI+VG   LP+  S FSL+ DGSGG+IIDSGT+
Sbjct: 523 KASESEIKTTPLIQNSAQPSFYYLSLEGISVGDTSLPIKKSTFSLHDDGSGGMIIDSGTT 582

Query: 318 ITYITEDAFDVLKPVFTSQTNLPAINSASIGLDLCFELPSHNKSKLDVPDLIFHFEGLDL 377
           ITY+ + AFD++   FTSQ NLP  NS S GL++CF LPS   + +DVP L+FHF+G DL
Sbjct: 583 ITYLEQSAFDLVAKEFTSQINLPVDNSGSTGLEVCFTLPS-GSTNIDVPKLVFHFDGADL 642

Query: 378 KLPVENYMVVNEELGIACLAMGAAGGLSIFGSMQHQNMLVLHDLEKKVVSFIPTQCSQL 432
           +LP ENYM+ +  +G+ACLAMG++ G+SIFG++Q QNMLVLHDLEK+ +SF+PTQC Q+
Sbjct: 643 ELPAENYMIADASMGLACLAMGSSSGMSIFGNIQQQNMLVLHDLEKETLSFLPTQCDQI 696

[Close]

BLAST of Lsi08G004290 vs. NCBI nr
Match: XP_034919474.1 (aspartic proteinase nepenthesin-1-like [Populus alba] >TKS10217.1 aspartyl protease family protein [Populus alba])

HSP 1 Score: 441.0 bits (1133), Expect = 1.2e-119
Identity = 220/419 (52.51%), Postives = 294/419 (70.17%), Query Frame = 0


Query: 18  SSEPALSESSLKPGFHFDLTHVDAARNFTKFEMLQRGIERDKTRMQKFNEMTM----NFE 77
           +S   L    ++ GF   L HVD+ +N TKFE +Q G++R + R+Q+F  M +    N E
Sbjct: 26  TSRRVLEHPKVQNGFRVKLKHVDSGKNLTKFERIQHGVKRGRHRLQRFKAMALVASSNSE 85

Query: 78  VRMPVVEQQGSYLMNLSLGTPSVSFSAIFDTGSELIWTQCKPCLQCFEQPTPVYDPAQSP 137
           +  PV+   G +LM L++GTP  ++SAI DTGS+LIWTQCKPC QCF+QPTP++DP +S 
Sbjct: 86  IDAPVLPGNGEFLMKLAIGTPPETYSAIMDTGSDLIWTQCKPCTQCFDQPTPIFDPKKSS 145

Query: 138 SFANATRAAALCQALTNSSSSNGCEYSVGYVDGSFTIGYLAFETLTLGEANQQVSTPNIA 197
           SF+  + ++ LC+AL  S+ S+GCEY  GY D S T G LA ETLT G    +VS P +A
Sbjct: 146 SFSKLSCSSKLCEALPQSTCSDGCEYLYGYGDYSSTQGILASETLTFG----KVSVPKVA 205

Query: 198 FGCGIRNQVKSLTQGAGIVGFSRGPLSLLSQLHIQKFSYCL-SPNGTGSLAFGSSSTSID 257
           FGCG  N+    +QG+G+VG  RGPLSL+SQL   KFSYCL S + T +      S +  
Sbjct: 206 FGCGEDNEGSGFSQGSGLVGLGRGPLSLVSQLEEPKFSYCLTSVDDTKASTLLMGSLASV 265

Query: 258 NTTNRAIKTTPLIQNPFDPSYYYLSLQGITVGQKFLPVPSSWFSLNADGSGGVIIDSGTS 317
             ++  IKTTPLIQN   PS+YYLSL+GI+VG   LP+  S FSL  DGSGG+IIDSGT+
Sbjct: 266 KASDSEIKTTPLIQNSAQPSFYYLSLEGISVGDTSLPIKKSTFSLQEDGSGGLIIDSGTT 325

Query: 318 ITYITEDAFDVLKPVFTSQTNLPAINSASIGLDLCFELPSHNKSKLDVPDLIFHFEGLDL 377
           ITY+ + AFD++   FTSQ NLP  NS S GL++CF LPS   + ++VP L+FHF+G DL
Sbjct: 326 ITYLEQSAFDLVAKEFTSQINLPVDNSGSTGLEVCFTLPS-GSTDIEVPKLVFHFDGADL 385

Query: 378 KLPVENYMVVNEELGIACLAMGAAGGLSIFGSMQHQNMLVLHDLEKKVVSFIPTQCSQL 432
           +LP ENYM+ +  +G+ACLAMG++ G+SIFG++Q QNMLVLHDLEK+ +SF+PTQC +L
Sbjct: 386 ELPAENYMIADASMGVACLAMGSSSGMSIFGNIQQQNMLVLHDLEKETLSFLPTQCDEL 439

[Close]

BLAST of Lsi08G004290 vs. NCBI nr
Match: KAG6738256.1 (hypothetical protein POTOM_057865 [Populus tomentosa])

HSP 1 Score: 441.0 bits (1133), Expect = 1.2e-119
Identity = 220/419 (52.51%), Postives = 294/419 (70.17%), Query Frame = 0


Query: 18  SSEPALSESSLKPGFHFDLTHVDAARNFTKFEMLQRGIERDKTRMQKFNEMTM----NFE 77
           +S   L    ++ GF   L HVD+ +N TKFE +Q G++R + R+Q+F  M +    N E
Sbjct: 26  TSRRVLEHPKVQNGFRVKLKHVDSGKNLTKFECIQHGVKRGRHRLQRFKAMALVASSNSE 85

Query: 78  VRMPVVEQQGSYLMNLSLGTPSVSFSAIFDTGSELIWTQCKPCLQCFEQPTPVYDPAQSP 137
           +  PV+   G +LM L++GTP  ++SAI DTGS+LIWTQCKPC QCF+QPTP++DP +S 
Sbjct: 86  IDAPVLPGNGEFLMKLAIGTPPETYSAIMDTGSDLIWTQCKPCTQCFDQPTPIFDPKKSS 145

Query: 138 SFANATRAAALCQALTNSSSSNGCEYSVGYVDGSFTIGYLAFETLTLGEANQQVSTPNIA 197
           SF+  + ++ LC+AL  S+ S+GCEY  GY D S T G LA ETLT G    +VS P +A
Sbjct: 146 SFSKLSCSSKLCEALPQSTCSDGCEYLYGYGDYSSTQGILASETLTFG----KVSVPKVA 205

Query: 198 FGCGIRNQVKSLTQGAGIVGFSRGPLSLLSQLHIQKFSYCL-SPNGTGSLAFGSSSTSID 257
           FGCG  N+    +QG+G+VG  RGPLSL+SQL   KFSYCL S + T +      S +  
Sbjct: 206 FGCGEDNEGSGFSQGSGLVGLGRGPLSLVSQLEEPKFSYCLTSVDDTKASTLLMGSLASV 265

Query: 258 NTTNRAIKTTPLIQNPFDPSYYYLSLQGITVGQKFLPVPSSWFSLNADGSGGVIIDSGTS 317
             ++  IKTTPLIQN   PS+YYLSL+GI+VG   LP+  S FSL  DGSGG+IIDSGT+
Sbjct: 266 KASDSEIKTTPLIQNSAQPSFYYLSLEGISVGDTTLPIKKSTFSLQEDGSGGLIIDSGTT 325

Query: 318 ITYITEDAFDVLKPVFTSQTNLPAINSASIGLDLCFELPSHNKSKLDVPDLIFHFEGLDL 377
           ITY+ + AFD++   FTSQ NLP  NS S GL++CF LPS   + ++VP L+FHF+G DL
Sbjct: 326 ITYLEQSAFDLVAKEFTSQINLPVDNSGSTGLEVCFTLPS-GSTDIEVPKLVFHFDGADL 385

Query: 378 KLPVENYMVVNEELGIACLAMGAAGGLSIFGSMQHQNMLVLHDLEKKVVSFIPTQCSQL 432
           +LP ENYM+ +  +G+ACLAMG++ G+SIFG++Q QNMLVLHDLEK+ +SF+PTQC +L
Sbjct: 386 ELPAENYMIADASMGVACLAMGSSSGMSIFGNIQQQNMLVLHDLEKETLSFLPTQCDEL 439

[Close]

BLAST of Lsi08G004290 vs. NCBI nr
Match: KAG6737285.1 (hypothetical protein POTOM_058799 [Populus tomentosa])

HSP 1 Score: 440.7 bits (1132), Expect = 1.5e-119
Identity = 220/419 (52.51%), Postives = 294/419 (70.17%), Query Frame = 0


Query: 18  SSEPALSESSLKPGFHFDLTHVDAARNFTKFEMLQRGIERDKTRMQKFNEMTM----NFE 77
           +S   L    +  GF   L HVD+ +N TKFE +Q G++R + R+Q+F  M +    N E
Sbjct: 26  TSRRVLEHPKVLNGFRVKLKHVDSGKNLTKFERIQHGVKRGRHRLQRFKAMALVASSNSE 85

Query: 78  VRMPVVEQQGSYLMNLSLGTPSVSFSAIFDTGSELIWTQCKPCLQCFEQPTPVYDPAQSP 137
           +  PV+   G +LM L++GTP  ++SAI DTGS+LIWTQCKPC QCF+QPTP++DP +S 
Sbjct: 86  IDAPVLPGNGEFLMKLAIGTPPETYSAIMDTGSDLIWTQCKPCTQCFDQPTPIFDPKKSS 145

Query: 138 SFANATRAAALCQALTNSSSSNGCEYSVGYVDGSFTIGYLAFETLTLGEANQQVSTPNIA 197
           SF+  + ++ LC+AL  S+ S+GCEY  GY D S T G LA ETLT G    +VS P +A
Sbjct: 146 SFSKLSCSSKLCEALPQSTCSDGCEYLYGYGDYSSTQGILASETLTFG----KVSVPKVA 205

Query: 198 FGCGIRNQVKSLTQGAGIVGFSRGPLSLLSQLHIQKFSYCL-SPNGTGSLAFGSSSTSID 257
           FGCG  N+    +QG+G+VG  RGPLSL+SQL   KFSYCL S + T +      S +  
Sbjct: 206 FGCGEDNEGSGFSQGSGLVGLGRGPLSLVSQLEEPKFSYCLTSVDDTKASTLLMGSLASV 265

Query: 258 NTTNRAIKTTPLIQNPFDPSYYYLSLQGITVGQKFLPVPSSWFSLNADGSGGVIIDSGTS 317
            +++  IKTTPLIQN   PS+YYLSL+GI+VG   LP+  S FSL  DGSGG+IIDSGT+
Sbjct: 266 KSSDSEIKTTPLIQNSAQPSFYYLSLEGISVGDTSLPIKKSTFSLQEDGSGGLIIDSGTT 325

Query: 318 ITYITEDAFDVLKPVFTSQTNLPAINSASIGLDLCFELPSHNKSKLDVPDLIFHFEGLDL 377
           ITY+ + AFD++   FTSQ NLP  NS S GL++CF LPS   + ++VP L+FHF+G DL
Sbjct: 326 ITYLEQSAFDLVAKEFTSQINLPVDNSGSTGLEVCFTLPS-GSTDIEVPKLVFHFDGADL 385

Query: 378 KLPVENYMVVNEELGIACLAMGAAGGLSIFGSMQHQNMLVLHDLEKKVVSFIPTQCSQL 432
           +LP ENYM+ +  +G+ACLAMG++ G+SIFG++Q QNMLVLHDLEK+ +SF+PTQC +L
Sbjct: 386 ELPAENYMIADASMGVACLAMGSSSGMSIFGNIQQQNMLVLHDLEKETLSFVPTQCDEL 439

[Close]

BLAST of Lsi08G004290 vs. TAIR 10
Match: AT2G03200.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 387.9 bits (995), Expect = 1.1e-107
Identity = 212/452 (46.90%), Postives = 284/452 (62.83%), Query Frame = 0


Query: 6   FCFLLLLLLPILSSEPALSESSL-----KPGFHFDLTHVDAARNFTKFEMLQRGIERDKT 65
           F  L   L+ + SS  +L + +L     + GF   L HVD+ +N TK + +QRGI R   
Sbjct: 14  FLILFSCLISVSSSRRSLIDRTLPKNLPRSGFRLSLRHVDSGKNLTKIQKIQRGINRGFH 73

Query: 66  RMQKFNEMTM---------NFEVRMPVVEQQGSYLMNLSLGTPSVSFSAIFDTGSELIWT 125
           R+ +   + +            ++ P     G +LM LS+G P+V +SAI DTGS+LIWT
Sbjct: 74  RLNRLGAVAVLAVASKPDDTNNIKAPTHGGSGEFLMELSIGNPAVKYSAIVDTGSDLIWT 133

Query: 126 QCKPCLQCFEQPTPVYDPAQSPSFANATRAAALCQALTNSS---SSNGCEYSVGYVDGSF 185
           QCKPC +CF+QPTP++DP +S S++    ++ LC AL  S+     + CEY   Y D S 
Sbjct: 134 QCKPCTECFDQPTPIFDPEKSSSYSKVGCSSGLCNALPRSNCNEDKDACEYLYTYGDYSS 193

Query: 186 TIGYLAFETLTLGEANQQVSTPNIAFGCGIRNQVKSLTQGAGIVGFSRGPLSLLSQLHIQ 245
           T G LA ET T  + N   S   I FGCG+ N+    +QG+G+VG  RGPLSL+SQL   
Sbjct: 194 TRGLLATETFTFEDEN---SISGIGFGCGVENEGDGFSQGSGLVGLGRGPLSLISQLKET 253

Query: 246 KFSYCLS----PNGTGSLAFGSSSTSIDNTTNRAI-----KTTPLIQNPFDPSYYYLSLQ 305
           KFSYCL+       + SL  GS ++ I N T  ++     KT  L++NP  PS+YYL LQ
Sbjct: 254 KFSYCLTSIEDSEASSSLFIGSLASGIVNKTGASLDGEVTKTMSLLRNPDQPSFYYLELQ 313

Query: 306 GITVGQKFLPVPSSWFSLNADGSGGVIIDSGTSITYITEDAFDVLKPVFTSQTNLPAINS 365
           GITVG K L V  S F L  DG+GG+IIDSGT+ITY+ E AF VLK  FTS+ +LP  +S
Sbjct: 314 GITVGAKRLSVEKSTFELAEDGTGGMIIDSGTTITYLEETAFKVLKEEFTSRMSLPVDDS 373

Query: 366 ASIGLDLCFELPSHNKSKLDVPDLIFHFEGLDLKLPVENYMVVNEELGIACLAMGAAGGL 425
            S GLDLCF+LP   K+ + VP +IFHF+G DL+LP ENYMV +   G+ CLAMG++ G+
Sbjct: 374 GSTGLDLCFKLPDAAKN-IAVPKMIFHFKGADLELPGENYMVADSSTGVLCLAMGSSNGM 433

Query: 426 SIFGSMQHQNMLVLHDLEKKVVSFIPTQCSQL 432
           SIFG++Q QN  VLHDLEK+ VSF+PT+C +L
Sbjct: 434 SIFGNVQQQNFNVLHDLEKETVSFVPTECGKL 461

[Close]

BLAST of Lsi08G004290 vs. TAIR 10
Match: AT5G33340.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 235.0 bits (598), Expect = 1.2e-61
Identity = 156/451 (34.59%), Postives = 238/451 (52.77%), Query Frame = 0


Query: 3   MANFCFLLLLLLPILSSEPALSESSLKP--GFHFDLTHVDAARN------FTKFEMLQRG 62
           MA+    +LL L +LSS   LS ++ KP  GF  DL H D+ ++       T  + L+  
Sbjct: 1   MASLFSSVLLSLCLLSS-LFLSNANAKPKLGFTADLIHRDSPKSPFYNPMETSSQRLRNA 60

Query: 63  IERDKTRMQKFNEMTMNFEVRMPVVEQQGSYLMNLSLGTPSVSFSAIFDTGSELIWTQCK 122
           I R   R+  F E     + ++ +    G YLMN+S+GTP     AI DTGS+L+WTQC 
Sbjct: 61  IHRSVNRVFHFTEKDNTPQPQIDLTSNSGEYLMNVSIGTPPFPIMAIADTGSDLLWTQCA 120

Query: 123 PCLQCFEQPTPVYDPAQSPSFANATRAAALCQALTN----SSSSNGCEYSVGYVDGSFTI 182
           PC  C+ Q  P++DP  S ++ + + +++ C AL N    S++ N C YS+ Y D S+T 
Sbjct: 121 PCDDCYTQVDPLFDPKTSSTYKDVSCSSSQCTALENQASCSTNDNTCSYSLSYGDNSYTK 180

Query: 183 GYLAFETLTLGEAN-QQVSTPNIAFGCGIRNQVKSLTQGAGIVGFSRGPLSLLSQLHIQ- 242
           G +A +TLTLG ++ + +   NI  GCG  N      +G+GIVG   GP+SL+ QL    
Sbjct: 181 GNIAVDTLTLGSSDTRPMQLKNIIIGCGHNNAGTFNKKGSGIVGLGGGPVSLIKQLGDSI 240

Query: 243 --KFSYCLSP-----NGTGSLAFGSSSTSIDNTTNRAIKTTPLIQNPFDPSYYYLSLQGI 302
             KFSYCL P     + T  + FG+++      +   + +TPLI      ++YYL+L+ I
Sbjct: 241 DGKFSYCLVPLTSKKDQTSKINFGTNAI----VSGSGVVSTPLIAKASQETFYYLTLKSI 300

Query: 303 TVGQKFLPVPSSWFSLNADGSGGVIIDSGTSITYITEDAFDVLKPVFTSQTNLPAINSAS 362
           +VG K +       S +    G +IIDSGT++T +  + +  L+    S  +        
Sbjct: 301 SVGSKQIQYSG---SDSESSEGNIIIDSGTTLTLLPTEFYSELEDAVASSIDAEKKQDPQ 360

Query: 363 IGLDLCFELPSHNKSKLDVPDLIFHFEGLDLKLPVEN-YMVVNEELGIACLAMGAAGGLS 422
            GL LC+         L VP +  HF+G D+KL   N ++ V+E+L   C A   +   S
Sbjct: 361 SGLSLCYSA----TGDLKVPVITMHFDGADVKLDSSNAFVQVSEDL--VCFAFRGSPSFS 420

Query: 423 IFGSMQHQNMLVLHDLEKKVVSFIPTQCSQL 432
           I+G++   N LV +D   K VSF PT C+++
Sbjct: 421 IYGNVAQMNFLVGYDTVSKTVSFKPTDCAKM 437

[Close]

BLAST of Lsi08G004290 vs. TAIR 10
Match: AT1G64830.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 225.7 bits (574), Expect = 7.1e-59
Identity = 152/450 (33.78%), Postives = 242/450 (53.78%), Query Frame = 0


Query: 3   MANFCFLLLLLLPILSSEPALSESSLKPGFHFDLTHVDAARN------FTKFEMLQRGIE 62
           MA+  F  LL L +LS+  A      K GF  DL H D+ ++       T  + ++  I 
Sbjct: 1   MASLIFATLLSLLLLSNVNAYP----KDGFTIDLIHRDSPKSPFYNSAETSSQRMRNAIR 60

Query: 63  RD-KTRMQKFNEMTMNFEVRMPVVEQQGSYLMNLSLGTPSVSFSAIFDTGSELIWTQCKP 122
           R  ++ +Q  N+       +  +   +G YLMN+S+GTP V   AI DTGS+LIWTQC P
Sbjct: 61  RSARSTLQFSNDDASPNSPQSFITSNRGEYLMNISIGTPPVPILAIADTGSDLIWTQCNP 120

Query: 123 CLQCFEQPTPVYDPAQSPSFANATRAAALCQALTNSSSS---NGCEYSVGYVDGSFTIGY 182
           C  C++Q +P++DP +S ++   + +++ C+AL ++S S   N C Y++ Y D S+T G 
Sbjct: 121 CEDCYQQTSPLFDPKESSTYRKVSCSSSQCRALEDASCSTDENTCSYTITYGDNSYTKGD 180

Query: 183 LAFETLTLGEANQQ-VSTPNIAFGCGIRNQVKSLTQGAGIVGFSRGPLSLLSQLHIQ--- 242
           +A +T+T+G + ++ VS  N+  GCG  N       G+GI+G   G  SL+SQL      
Sbjct: 181 VAVDTVTMGSSGRRPVSLRNMIIGCGHENTGTFDPAGSGIIGLGGGSTSLVSQLRKSING 240

Query: 243 KFSYCLSP-----NGTGSLAFGSSSTSIDNTTNRAIKTTPLIQNPFDP-SYYYLSLQGIT 302
           KFSYCL P       T  + FG++       +   + +T +++   DP +YY+L+L+ I+
Sbjct: 241 KFSYCLVPFTSETGLTSKINFGTNGI----VSGDGVVSTSMVKK--DPATYYFLNLEAIS 300

Query: 303 VGQKFLPVPSSWFSLNADGSGGVIIDSGTSITYITEDAFDVLKPVFTSQTNLPAINSASI 362
           VG K +   S+ F     G G ++IDSGT++T +  + +  L+ V  S      +     
Sbjct: 301 VGSKKIQFTSTIF---GTGEGNIVIDSGTTLTLLPSNFYYELESVVASTIKAERVQDPDG 360

Query: 363 GLDLCFELPSHNKSKLDVPDLIFHFEGLDLKL-PVENYMVVNEELGIACLAMGAAGGLSI 422
            L LC+     + S   VPD+  HF+G D+KL  +  ++ V+E+  ++C A  A   L+I
Sbjct: 361 ILSLCY----RDSSSFKVPDITVHFKGGDVKLGNLNTFVAVSED--VSCFAFAANEQLTI 420

Query: 423 FGSMQHQNMLVLHDLEKKVVSFIPTQCSQL 432
           FG++   N LV +D     VSF  T CSQ+
Sbjct: 421 FGNLAQMNFLVGYDTVSGTVSFKKTDCSQM 431

[Close]

BLAST of Lsi08G004290 vs. TAIR 10
Match: AT2G35615.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 218.0 bits (554), Expect = 1.5e-56
Identity = 146/447 (32.66%), Postives = 238/447 (53.24%), Query Frame = 0


Query: 11  LLLLPILSSEPALSESSLKPGFHFDLTHVDAARN------FTKFEMLQRGIERDKTRMQK 70
           +LL   L     LS S     F  +L H D+  +       T  + L     R  +R ++
Sbjct: 5   ILLCFFLFFSVTLSSSGHPKNFSVELIHRDSPLSPIYNPQITVTDRLNAAFLRSVSRSRR 64

Query: 71  FNEMTMNFEVRMPVVEQQGSYLMNLSLGTPSVSFSAIFDTGSELIWTQCKPCLQCFEQPT 130
           FN      +++  ++   G + M++++GTP +   AI DTGS+L W QCKPC QC+++  
Sbjct: 65  FNHQLSQTDLQSGLIGADGEFFMSITIGTPPIKVFAIADTGSDLTWVQCKPCQQCYKENG 124

Query: 131 PVYDPAQSPSFANATRAAALCQALTNS-----SSSNGCEYSVGYVDGSFTIGYLAFETLT 190
           P++D  +S ++ +    +  CQAL+++      S+N C+Y   Y D SF+ G +A ET++
Sbjct: 125 PIFDKKKSSTYKSEPCDSRNCQALSSTERGCDESNNICKYRYSYGDQSFSKGDVATETVS 184

Query: 191 LGEAN-QQVSTPNIAFGCGIRNQVKSLTQGAGIVGFSRGPLSLLSQLH---IQKFSYCLS 250
           +  A+   VS P   FGCG  N       G+GI+G   G LSL+SQL     +KFSYCLS
Sbjct: 185 IDSASGSPVSFPGTVFGCGYNNGGTFDETGSGIIGLGGGHLSLISQLGSSISKKFSYCLS 244

Query: 251 -----PNGTGSLAFGSSSTSIDNTTNRAIKTTPLIQNPFDP-SYYYLSLQGITVGQKFLP 310
                 NGT  +  G++S     + +  + +TPL+    +P +YYYL+L+ I+VG+K +P
Sbjct: 245 HKSATTNGTSVINLGTNSIPSSLSKDSGVVSTPLVDK--EPLTYYYLTLEAISVGKKKIP 304

Query: 311 VPSSWFSLNADG-----SGGVIIDSGTSITYITEDAFDVL-KPVFTSQTNLPAINSASIG 370
              S ++ N DG     SG +IIDSGT++T +    FD     V  S T    ++     
Sbjct: 305 YTGSSYNPNDDGILSETSGNIIIDSGTTLTLLEAGFFDKFSSAVEESVTGAKRVSDPQGL 364

Query: 371 LDLCFELPSHNKSKLDVPDLIFHFEGLDLKL-PVENYMVVNEELGIACLAMGAAGGLSIF 430
           L  CF+  S   +++ +P++  HF G D++L P+  ++ ++E++   CL+M     ++I+
Sbjct: 365 LSHCFKSGS---AEIGLPEITVHFTGADVRLSPINAFVKLSEDM--VCLSMVPTTEVAIY 424

[Close]

BLAST of Lsi08G004290 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 217.2 bits (552), Expect = 2.5e-56
Identity = 153/444 (34.46%), Postives = 220/444 (49.55%), Query Frame = 0


Query: 19  SEPALSESSLKPGFHFDLTHVDAARNF---TKFEMLQRGIERDKTRMQKFNEMT-----M 78
           ++ +LSES+     H  L+HVDA  +F   +  ++    ++RD  R++    +       
Sbjct: 50  TDESLSESTTSLSVH--LSHVDALSSFSDASPADLFNLRLQRDSLRVKSITSLAAVSTGR 109

Query: 79  NFEVRMP-------------VVEQQGSYLMNLSLGTPSVSFSAIFDTGSELIWTQCKPCL 138
           N   R P             + +  G Y M L +GTP+ +   + DTGS+++W QC PC 
Sbjct: 110 NATKRTPRTAGGFSGAVISGLSQGSGEYFMRLGVGTPATNVYMVLDTGSDVVWLQCSPCK 169

Query: 139 QCFEQPTPVYDPAQSPSFANATRAAALCQALTNSSS-----SNGCEYSVGYVDGSFTIGY 198
            C+ Q   ++DP +S +FA     + LC+ L +SS      S  C Y V Y DGSFT G 
Sbjct: 170 ACYNQTDAIFDPKKSKTFATVPCGSRLCRRLDDSSECVTRRSKTCLYQVSYGDGSFTEGD 229

Query: 199 LAFETLTLGEANQQVSTPNIAFGCGIRNQVKSLTQGAGIVGFSRGPLSLLSQL---HIQK 258
            + ETLT   A       ++  GCG  N+       AG++G  RG LS  SQ    +  K
Sbjct: 230 FSTETLTFHGAR----VDHVPLGCGHDNE-GLFVGAAGLLGLGRGGLSFPSQTKNRYNGK 289

Query: 259 FSYCLSPNGTGSLAFGSSSTSI--DNTTNRAIKTTPLIQNPFDPSYYYLSLQGITVGQKF 318
           FSYCL    +   +    ST +  +    +    TPL+ NP   ++YYL L GI+VG   
Sbjct: 290 FSYCLVDRTSSGSSSKPPSTIVFGNAAVPKTSVFTPLLTNPKLDTFYYLQLLGISVGGSR 349

Query: 319 LP-VPSSWFSLNADGSGGVIIDSGTSITYITEDAFDVLKPVF-TSQTNLPAINSASIGLD 378
           +P V  S F L+A G+GGVIIDSGTS+T +T+ A+  L+  F    T L    S S+  D
Sbjct: 350 VPGVSESQFKLDATGNGGVIIDSGTSVTRLTQPAYVALRDAFRLGATKLKRAPSYSL-FD 409

Query: 379 LCFELPSHNKSKLDVPDLIFHFEGLDLKLPVENYMVVNEELGIACLAM-GAAGGLSIFGS 429
            CF+L      K  VP ++FHF G ++ LP  NY++     G  C A  G  G LSI G+
Sbjct: 410 TCFDLSGMTTVK--VPTVVFHFGGGEVSLPASNYLIPVNTEGRFCFAFAGTMGSLSIIGN 469

The following BLAST results are available for this feature:

BLAST of Lsi08G004290 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q766C3	2.9e-97	45.50	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]
Q766C2	7.1e-96	45.70	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]
Q6XBF8	1.6e-60	34.59	Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1	[more]
Q7XV21	2.1e-55	32.69	Aspartyl protease 37 OS=Oryza sativa subsp. japonica OX=39947 GN=AP37 PE=3 SV=2	[more]
Q3EBM5	2.1e-55	32.66	Probable aspartic protease At2g35615 OS=Arabidopsis thaliana OX=3702 GN=At2g3561...	[more]

BLAST of Lsi08G004290 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A2N9HF56	2.6e-125	55.58	Peptidase A1 domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS4...	[more]
A0A4U5QLX0	5.6e-120	52.51	Aspartyl protease family protein OS=Populus alba OX=43335 GN=D5086_0000085760 PE...	[more]
A0A4U5R6H0	7.3e-120	52.51	Aspartyl protease family protein OS=Populus alba OX=43335 GN=D5086_0000004600 PE...	[more]
B9N2J4	9.5e-120	52.51	Peptidase A1 domain-containing protein OS=Populus trichocarpa OX=3694 GN=POPTR_0...	[more]
A0A6M2F5X8	2.1e-119	52.51	Peptidase A1 domain-containing protein OS=Populus davidiana OX=266767 PE=3 SV=1	[more]

BLAST of Lsi08G004290 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038886455.1	3.0e-208	85.48	aspartic proteinase nepenthesin-2-like [Benincasa hispida]	[more]
KAF9660916.1	8.8e-120	52.74	hypothetical protein SADUNF_Sadunf19G0013400 [Salix dunnii]	[more]
XP_034919474.1	1.2e-119	52.51	aspartic proteinase nepenthesin-1-like [Populus alba] >TKS10217.1 aspartyl prote...	[more]
KAG6738256.1	1.2e-119	52.51	hypothetical protein POTOM_057865 [Populus tomentosa]	[more]
KAG6737285.1	1.5e-119	52.51	hypothetical protein POTOM_058799 [Populus tomentosa]	[more]

BLAST of Lsi08G004290 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G03200.1	1.1e-107	46.90	Eukaryotic aspartyl protease family protein	[more]
AT5G33340.1	1.2e-61	34.59	Eukaryotic aspartyl protease family protein	[more]
AT1G64830.1	7.1e-59	33.78	Eukaryotic aspartyl protease family protein	[more]
AT2G35615.1	1.5e-56	32.66	Eukaryotic aspartyl protease family protein	[more]
AT3G61820.1	2.5e-56	34.46	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001461	Aspartic peptidase A1 family	PRINTS	PR00792	PEPSIN	coord: 91..111 score: 44.74 coord: 305..316 score: 41.72
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 85..246 e-value: 6.4E-45 score: 153.5
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 248..433 e-value: 3.0E-51 score: 175.7
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 59..246 e-value: 8.8E-49 score: 168.1
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 81..430
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 274..423 e-value: 3.2E-29 score: 101.8
None	No IPR available	PANTHER	PTHR47967	OS07G0603500 PROTEIN-RELATED	coord: 16..430
None	No IPR available	PANTHER	PTHR47967:SF23	OS08G0469000 PROTEIN	coord: 16..430
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 305..316
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 85..424 score: 37.85601
IPR034161	Pepsin-like domain, plant	CDD	cd05476	pepsin_A_like_plant	coord: 85..428 e-value: 1.75978E-97 score: 291.089

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi08G004290.1	Lsi08G004290.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0004190	aspartic-type endopeptidase activity