Lsi08G003860 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi08G003860
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Mannan endo-1,4-beta-mannosidase
Location	chr08: 10651352 .. 10653150 (+)
RNA-Seq Expression	Lsi08G003860
Synteny	Lsi08G003860

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TATTCCTTACCCTTATCAACGCAAGGGAGATGGATCATTGATTCGCAAACAGGACGTCGAGTGAAACTTGTGTGTGTGAATTGGCCCTCCCACACACAGAGCATGCTTGCAGAAGGTCTCAACCATAGATCGTTAAAAGATCTAGCAGACGAAGCAATCAAGTTAAGATTCAATTGTGTACGACTCACATATGCAACCCACATGTTCACTCGCTACGCAAATAGGACGATTGAAGAAAACTTCGATCTTCTTGATTTAAAACAAGCAAAAGCTGGATTGGCTCAATATAATCCTTTTGTGTTGAACAAGACTATTCCAGAAGCTTATGAAGCTGTTGTTGATGTGCTGGGGGCAAGTGGTCTGATGGTGATTGCTGACAATCACATGAGTCAGCCAAGATGGTGTTGTTCTCTTGACGATGGCAATGGCTTCTTTGGGAACCGCTATTTTGACCCTCAAGAATGGCTACAAGGTCTTAGCTTAGTTGCTCAACGCTTTAACAACAAATCTACGGTATGTAACATCAAAAGATTTTCTTAATTTCTTGAAACTAAACTCAAGTATTTAAAAACTTCTTTAATATTTCATTAAACAAAGGAAGTCTTTTTATGAATTTTTAGGTGGTAGGGATGAGTTTACGAAATGAGATACGAGGAATGATGGAAAATGCAAATGATTGGAACAACTATATAACTCAAGGAGTAACCACAATCCATAACATAAATTCGGCAGTTTTAGTCATTGTTTCAGGTTTAAATTATGACAATGATCTCCGATGCTTAAAGGAGAAGCCTTTGACTGTAAAGACCTTAGACAATAAGTTGGTTTTTGAGGTACACTTGTATTCTTTTAGTGGAGATTCTGAGAGCAAGTTTGTGCAACAACCATTGAACAATATTTGTGCAAATATTATGAATGGCTTTATAGACCATGCTGAGTTTGTAATTGAAGGATCAAACCCATTTCCTTTATTTGTTAGTGAATATGGGTATGATCAAAGAGAGGTTGACGATGCAGAAAATCGATTCATGAGCTGCTTCACAGCCCATCTTGCACAAAAAGATTTGGACTGGGCATTGTGGACATGGCAAGGTAGTTATTATTATAGAGAAGGTCAAGCAGAGTCTACAGAAACGTTTGGAGTGCTCGAAGCTAATTGGACTCAAATTAAGAACCCTAACTTTGTCAAGAAGTTTCAATTACTGCAGACCATGTTGCAAGGTAACTATCTTAATGATTTTGAATTTATTCAAGGATCTATAACTAGTAAACTAATATGATTTGTGAAAATAAATGTTAAATAGTTCGATTAGGTTTTATAAAACTTGTTAAATAACAATTTGGATAACATTTTTTTATGGTACACAGATCCAAATTCCAATGCATCATTCTCATATGTTATATATCATCCACAAAGTGGCCAATGTATCCAAGTCTCTAATGACAACAAAGAAATTTTTCTCACCAATTGCTCCACCTCAACTCGATGGAGTCATGACAATGATAGCACTCCAATTACAATGTCAAACACTGGTTTGTGCTTGAAGGCTAGTGGAGAAGGCCTTGAGGCATCTCTTTCAACTGACTGCTCTAGCAAACAAAGTGTTTGGAGTGCCATTTCAAGCTCTAAGCTGCATTTGGCAACTTTAACTGAAGATGGCAAGAGTCTTTGTCTGCAGATTGAAAGTTCAAATTCTTCAAAAATTGTGACCAGCTCTTGTATTTGCACGAATGGTGATCCAACTTGCCTCCAAGACACCCAAAGCCAATGGTTTGAACTTGTTGGAACCAACACATTG

mRNA sequence

TATTCCTTACCCTTATCAACGCAAGGGAGATGGATCATTGATTCGCAAACAGGACGTCGAGTGAAACTTGTGTGTGTGAATTGGCCCTCCCACACACAGAGCATGCTTGCAGAAGGTCTCAACCATAGATCGTTAAAAGATCTAGCAGACGAAGCAATCAAGTTAAGATTCAATTGTGTACGACTCACATATGCAACCCACATGTTCACTCGCTACGCAAATAGGACGATTGAAGAAAACTTCGATCTTCTTGATTTAAAACAAGCAAAAGCTGGATTGGCTCAATATAATCCTTTTGTGTTGAACAAGACTATTCCAGAAGCTTATGAAGCTGTTGTTGATGTGCTGGGGGCAAGTGGTCTGATGGTGATTGCTGACAATCACATGAGTCAGCCAAGATGGTGTTGTTCTCTTGACGATGGCAATGGCTTCTTTGGGAACCGCTATTTTGACCCTCAAGAATGGCTACAAGGTCTTAGCTTAGTTGCTCAACGCTTTAACAACAAATCTACGGTGGTAGGGATGAGTTTACGAAATGAGATACGAGGAATGATGGAAAATGCAAATGATTGGAACAACTATATAACTCAAGGAGTAACCACAATCCATAACATAAATTCGGCAGTTTTAGTCATTGTTTCAGGTTTAAATTATGACAATGATCTCCGATGCTTAAAGGAGAAGCCTTTGACTGTAAAGACCTTAGACAATAAGTTGGTTTTTGAGGTACACTTGTATTCTTTTAGTGGAGATTCTGAGAGCAAGTTTGTGCAACAACCATTGAACAATATTTGTGCAAATATTATGAATGGCTTTATAGACCATGCTGAGTTTGTAATTGAAGGATCAAACCCATTTCCTTTATTTGTTAGTGAATATGGGTATGATCAAAGAGAGGTTGACGATGCAGAAAATCGATTCATGAGCTGCTTCACAGCCCATCTTGCACAAAAAGATTTGGACTGGGCATTGTGGACATGGCAAGGTAGTTATTATTATAGAGAAGGTCAAGCAGAGTCTACAGAAACGTTTGGAGTGCTCGAAGCTAATTGGACTCAAATTAAGAACCCTAACTTTGTCAAGAAGTTTCAATTACTGCAGACCATGTTGCAAGATCCAAATTCCAATGCATCATTCTCATATGTTATATATCATCCACAAAGTGGCCAATGTATCCAAGTCTCTAATGACAACAAAGAAATTTTTCTCACCAATTGCTCCACCTCAACTCGATGGAGTCATGACAATGATAGCACTCCAATTACAATGTCAAACACTGGTTTGTGCTTGAAGGCTAGTGGAGAAGGCCTTGAGGCATCTCTTTCAACTGACTGCTCTAGCAAACAAAGTGTTTGGAGTGCCATTTCAAGCTCTAAGCTGCATTTGGCAACTTTAACTGAAGATGGCAAGAGTCTTTGTCTGCAGATTGAAAGTTCAAATTCTTCAAAAATTGTGACCAGCTCTTGTATTTGCACGAATGGTGATCCAACTTGCCTCCAAGACACCCAAAGCCAATGGTTTGAACTTGTTGGAACCAACACATTG

Coding sequence (CDS)

TATTCCTTACCCTTATCAACGCAAGGGAGATGGATCATTGATTCGCAAACAGGACGTCGAGTGAAACTTGTGTGTGTGAATTGGCCCTCCCACACACAGAGCATGCTTGCAGAAGGTCTCAACCATAGATCGTTAAAAGATCTAGCAGACGAAGCAATCAAGTTAAGATTCAATTGTGTACGACTCACATATGCAACCCACATGTTCACTCGCTACGCAAATAGGACGATTGAAGAAAACTTCGATCTTCTTGATTTAAAACAAGCAAAAGCTGGATTGGCTCAATATAATCCTTTTGTGTTGAACAAGACTATTCCAGAAGCTTATGAAGCTGTTGTTGATGTGCTGGGGGCAAGTGGTCTGATGGTGATTGCTGACAATCACATGAGTCAGCCAAGATGGTGTTGTTCTCTTGACGATGGCAATGGCTTCTTTGGGAACCGCTATTTTGACCCTCAAGAATGGCTACAAGGTCTTAGCTTAGTTGCTCAACGCTTTAACAACAAATCTACGGTGGTAGGGATGAGTTTACGAAATGAGATACGAGGAATGATGGAAAATGCAAATGATTGGAACAACTATATAACTCAAGGAGTAACCACAATCCATAACATAAATTCGGCAGTTTTAGTCATTGTTTCAGGTTTAAATTATGACAATGATCTCCGATGCTTAAAGGAGAAGCCTTTGACTGTAAAGACCTTAGACAATAAGTTGGTTTTTGAGGTACACTTGTATTCTTTTAGTGGAGATTCTGAGAGCAAGTTTGTGCAACAACCATTGAACAATATTTGTGCAAATATTATGAATGGCTTTATAGACCATGCTGAGTTTGTAATTGAAGGATCAAACCCATTTCCTTTATTTGTTAGTGAATATGGGTATGATCAAAGAGAGGTTGACGATGCAGAAAATCGATTCATGAGCTGCTTCACAGCCCATCTTGCACAAAAAGATTTGGACTGGGCATTGTGGACATGGCAAGGTAGTTATTATTATAGAGAAGGTCAAGCAGAGTCTACAGAAACGTTTGGAGTGCTCGAAGCTAATTGGACTCAAATTAAGAACCCTAACTTTGTCAAGAAGTTTCAATTACTGCAGACCATGTTGCAAGATCCAAATTCCAATGCATCATTCTCATATGTTATATATCATCCACAAAGTGGCCAATGTATCCAAGTCTCTAATGACAACAAAGAAATTTTTCTCACCAATTGCTCCACCTCAACTCGATGGAGTCATGACAATGATAGCACTCCAATTACAATGTCAAACACTGGTTTGTGCTTGAAGGCTAGTGGAGAAGGCCTTGAGGCATCTCTTTCAACTGACTGCTCTAGCAAACAAAGTGTTTGGAGTGCCATTTCAAGCTCTAAGCTGCATTTGGCAACTTTAACTGAAGATGGCAAGAGTCTTTGTCTGCAGATTGAAAGTTCAAATTCTTCAAAAATTGTGACCAGCTCTTGTATTTGCACGAATGGTGATCCAACTTGCCTCCAAGACACCCAAAGCCAATGGTTTGAACTTGTTGGAACCAACACATTG

Protein sequence

YSLPLSTQGRWIIDSQTGRRVKLVCVNWPSHTQSMLAEGLNHRSLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFVLNKTIPEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLSLVAQRFNNKSTVVGMSLRNEIRGMMENANDWNNYITQGVTTIHNINSAVLVIVSGLNYDNDLRCLKEKPLTVKTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEFVIEGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAESTETFGVLEANWTQIKNPNFVKKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQVSNDNKEIFLTNCSTSTRWSHDNDSTPITMSNTGLCLKASGEGLEASLSTDCSSKQSVWSAISSSKLHLATLTEDGKSLCLQIESSNSSKIVTSSCICTNGDPTCLQDTQSQWFELVGTNTL

Homology

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BLAST of Lsi08G003860 vs. ExPASy Swiss-Prot
Match: C0HLA0 (Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 414.5 bits (1064), Expect = 1.8e-114
Identity = 221/528 (41.86%), Postives = 320/528 (60.61%), Query Frame = 0


Query: 1   YSLPLSTQGRWIIDSQTGRRVKLVCVNWPSHTQSMLAEGLNHRSLKDLADEAIKLRFNCV 60
           +SLPL T+GRWI+D  TG RVKL CVNW  H +  L EGLN   +  +A     L FNCV
Sbjct: 27  HSLPLLTRGRWIVDEATGLRVKLACVNWVGHLEPGLPEGLNRLPVATVAHTISSLGFNCV 86

Query: 61  RLTYATHMFTR--YANRTIEENFDLLDLKQAKAGLAQYNPFVLNKTIPEAYEAVVDVLGA 120
           RLTY+ HM TR  Y N T+ + F  L+L +A +G+   NP +L+     AY  VV  L  
Sbjct: 87  RLTYSIHMLTRTSYTNATVAQTFARLNLTEAASGIEHNNPELLDLGHVAAYHHVVAALSE 146

Query: 121 SGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLSLVAQRFNNKSTVVGMSLR 180
           +G+MVI DNH+S+P+WCC++DDGNGFFG+RYF+P  W++GL L+A  FNN   VV MSLR
Sbjct: 147 AGVMVILDNHVSKPKWCCAVDDGNGFFGDRYFNPNTWVEGLGLMATYFNNTPNVVAMSLR 206

Query: 181 NEIRGMMENANDWNNYITQGVTTIHNINSAVLVIVSGLNYDNDLRCLKEKPLTVKTLDNK 240
           NE+RG       W+ ++  G  T+H  N  VLVI+SGL +D DL  L   P+T+     K
Sbjct: 207 NELRGNRSTPISWSRHMQWGAATVHKANPKVLVILSGLQFDTDLSFLPVLPVTL-PFKEK 266

Query: 241 LVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEFVIEGSN--PFPLFVSEYGYD 300
           +V+E H YSF     +       N++C N    F  +  FV   +N    PLF+SE+G D
Sbjct: 267 IVYEGHWYSFGVPWRTGLP----NDVCKNETGRFKSNVGFVTSSANATAAPLFMSEFGID 326

Query: 301 QREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQ---AESTETFGVLEANWTQ 360
           QR V+D +NR+++C  A+LA++DLDWALWT  GSYYYR  +    +  ET+G    +W++
Sbjct: 327 QRYVNDNDNRYLNCILAYLAEEDLDWALWTMGGSYYYRSDKQPVKDFEETYGFFNHDWSR 386

Query: 361 IKNPNFVKKFQLLQTMLQDPN-SNASFSYVIYHPQSGQCIQVSNDNKEIFLTNC-STSTR 420
           I+NP+F+ + + +Q  +QDP  +   +  +IYHP SG C++ S     + L +C S  +R
Sbjct: 387 IRNPDFISRLKEIQQPIQDPYLAPGPYYQIIYHPASGLCVE-SGIGNTVHLGSCQSVRSR 446

Query: 421 WSHD-NDSTPITMSNTGLCLKASGEGLEASLSTDCSS-KQSVWSAISSSKLHLAT--LTE 480
           W++D +   PI +  +  C+   G GL A ++ +CS+   ++WS +SS++L L T  L +
Sbjct: 447 WNYDASVKGPIGLMGSSSCISTQGNGLPAIMTENCSAPNNTLWSTVSSAQLQLGTRVLGK 506

Query: 481 DGKSLCLQIESSNSSKIVTSSCICTNGDPTCLQ--DTQSQWFELVGTN 514
           DGK   + ++ S S  I T+ CIC   D  C    + + QWF+++ TN
Sbjct: 507 DGKEKWMCLDGSKSPLISTNECICIT-DSHCYPKLNPEKQWFKVITTN 547

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BLAST of Lsi08G003860 vs. ExPASy Swiss-Prot
Match: P23548 (Endoglucanase OS=Paenibacillus polymyxa OX=1406 PE=3 SV=2)

HSP 1 Score: 81.3 bits (199), Expect = 3.6e-14
Identity = 86/376 (22.87%), Postives = 152/376 (40.43%), Query Frame = 0


Query: 7   TQGRWIIDSQTGRRVKLVCVNW-PSHTQSMLAEGLNHRSLKDLADEAIKLRFNCVRLTYA 66
           TQG  I+D ++G+      +NW    T +    GL  RS+ D+ D+  K  +N +RL Y+
Sbjct: 41  TQGNKIVD-ESGKEAAFNGLNWFGLETPNYTLHGLWSRSMDDMLDQVKKEGYNLIRLPYS 100

Query: 67  THMF---TRYANRTIEENFDLLDLKQAKAGLAQYNPF-VLNKTIPEAYEAVVDVLGASGL 126
             +F   +R  +    +N DL+ L          NP  +++K I +A        G  G+
Sbjct: 101 NQLFDSSSRPDSIDYHKNPDLVGL----------NPIQIMDKLIEKA--------GQRGI 160

Query: 127 MVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQ----EWLQGLSLVAQRFNNKSTVVGMSL 186
            +I D H            G+G     ++  Q     W+    ++A R+ N  TV+G  L
Sbjct: 161 QIILDRHR----------PGSGGQSELWYTSQYPESRWISDWKMLADRYKNNPTVIGADL 220

Query: 187 RNEIRGMME----NAN-DWNNYITQGVTTIHNINSAVLVIVSGLNYD-----------ND 246
            NE  G       NA+ DW     +    I ++N   L++V G++++            +
Sbjct: 221 HNEPHGQASWGTGNASTDWRLAAQRAGNAILSVNPNWLILVEGVDHNVQGNNSQYWWGGN 280

Query: 247 LRCLKEKPLTVKTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEFVIE 306
           L  +   P+ V  + N++V+  H Y   G S   +   P      + +    D     I 
Sbjct: 281 LTGVANYPV-VLDVPNRVVYSPHDYG-PGVSSQPWFNDP---AFPSNLPAIWDQTWGYIS 340

Query: 307 GSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAEST 358
             N  P+ V E+G    ++   E ++ +    ++   +L +  W+             S 
Sbjct: 341 KQNIAPVLVGEFGGRNVDLSCPEGKWQNALVHYIGANNLYFTYWSL---------NPNSG 373

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BLAST of Lsi08G003860 vs. ExPASy Swiss-Prot
Match: P19487 (Major extracellular endoglucanase OS=Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25) OX=190485 GN=engXCA PE=1 SV=2)

HSP 1 Score: 73.9 bits (180), Expect = 5.8e-12
Identity = 94/384 (24.48%), Postives = 152/384 (39.58%), Query Frame = 0


Query: 10  RWIIDSQTGRRVKLVCVN-WPSHTQSMLAEGLNHRSLKDLADEAIKLRFNCVRLTYATHM 69
           R I+D  +G+ V+L  VN +   T + +  GL  R+ KD+  +   L FN VRL +    
Sbjct: 32  RQIVD-DSGKVVQLKGVNVFGFETGNHVMHGLWARNWKDMIVQMQGLGFNAVRLPFCPAT 91

Query: 70  F---TRYANRTIEENFDLLDLKQAKAGLAQYNPFVLNKTIPEAYEAVVDVLGASGLMVIA 129
               T  A+     N DL  L   +         +L+K I E          A G+ V+ 
Sbjct: 92  LRSDTMPASIDYSRNADLQGLTSLQ---------ILDKVIAE--------FNARGMYVLL 151

Query: 130 DNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLSLVAQRFNNKSTVVGMSLRNEIRGMM 189
           D+H      C  + +   ++   Y + Q WL  L  VA R+ N   V+G+ L+NE  G  
Sbjct: 152 DHHTPD---CAGISE--LWYTGSYTEAQ-WLADLRFVANRYKNVPYVLGLDLKNEPHGAA 211

Query: 190 -----ENANDWNNYITQGVTTIHNINSAVLVIVSGLN------------YDNDLRCLKEK 249
                  A DWN    +G   +  +    L+ V G+             +  +L+ L   
Sbjct: 212 TWGTGNAATDWNKAAERGSAAVLAVAPKWLIAVEGITDNPVCSTNGGIFWGGNLQPLACT 271

Query: 250 PLTVKTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGF--IDHAEFVIEGSNPF 309
           PL +    N+L+   H+Y         FVQ   N+  +N  N    I    F  + +   
Sbjct: 272 PLNIPA--NRLLLAPHVY-----GPDVFVQSYFND--SNFPNNMPAIWERHFG-QFAGTH 331

Query: 310 PLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWAL-WTWQGSYYYREGQAESTETFG 369
            L + E+G    E D  +  +      +L  K ++    W+W            S +T G
Sbjct: 332 ALLLGEFGGKYGEGDARDKTWQDALVKYLRSKGINQGFYWSW---------NPNSGDTGG 368

[Close]

BLAST of Lsi08G003860 vs. ExPASy TrEMBL
Match: A0A0A0K853 (Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_6G028440 PE=3 SV=1)

HSP 1 Score: 978.0 bits (2527), Expect = 1.5e-281
Identity = 472/516 (91.47%), Postives = 491/516 (95.16%), Query Frame = 0


Query: 1   YSLPLSTQGRWIIDSQTGRRVKLVCVNWPSHTQSMLAEGLNHRSLKDLADEAIKLRFNCV 60
           YSLPLST GRWIIDSQ+G+RVKLVCVNWPSHTQSML EGLNHR LK+LADEAIKLRFNCV
Sbjct: 23  YSLPLSTHGRWIIDSQSGKRVKLVCVNWPSHTQSMLIEGLNHRPLKELADEAIKLRFNCV 82

Query: 61  RLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFVLNKTIPEAYEAVVDVLGASG 120
           RLTYATHMFTRYANRT+EENFDLLDL+QAKAGLAQYNPFVLNKTI EAYEAVVDVLGASG
Sbjct: 83  RLTYATHMFTRYANRTVEENFDLLDLEQAKAGLAQYNPFVLNKTIAEAYEAVVDVLGASG 142

Query: 121 LMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLSLVAQRFNNKSTVVGMSLRNE 180
           LMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLSLVAQRFNNKSTVVGMSLRNE
Sbjct: 143 LMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLSLVAQRFNNKSTVVGMSLRNE 202

Query: 181 IRGMMENANDWNNYITQGVTTIHNINSAVLVIVSGLNYDNDLRCLKEKPLTVKTLDNKLV 240
           +RGMMENANDWNNY+TQGVTTIH IN AVLVIVSGLNYDNDLRCLK+KPL V TLDNKL 
Sbjct: 203 LRGMMENANDWNNYVTQGVTTIHKINPAVLVIVSGLNYDNDLRCLKDKPLNVSTLDNKLA 262

Query: 241 FEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEFVIEGSNPFPLFVSEYGYDQREV 300
           FEVHLYSFSGDSESKFVQQPLNNICA IM+ FIDHAEFVIEG NPFPLFVSEYGYDQREV
Sbjct: 263 FEVHLYSFSGDSESKFVQQPLNNICAKIMHEFIDHAEFVIEGPNPFPLFVSEYGYDQREV 322

Query: 301 DDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAESTETFGVLEANWTQIKNPNFV 360
           DDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAE  ETFGVL++NWTQIKNPNFV
Sbjct: 323 DDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAELAETFGVLDSNWTQIKNPNFV 382

Query: 361 KKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQVSNDNKEIFLTNCSTSTRWSHDNDSTP 420
           +KFQLLQTMLQDP SNASFSYVIYH QSGQCI+VSNDNKEIFLTNCSTS+RWSHDNDSTP
Sbjct: 383 QKFQLLQTMLQDPYSNASFSYVIYHVQSGQCIEVSNDNKEIFLTNCSTSSRWSHDNDSTP 442

Query: 421 ITMSNTGLCLKASGEGLEASLSTDCSSKQSVWSAISSSKLHLATLTEDGKSLCLQ-IESS 480
           I MS+TGLCLKASGEGLEASLSTDC  KQS+WSAIS+S LHL T+TEDGKSLCLQ IESS
Sbjct: 443 IKMSSTGLCLKASGEGLEASLSTDCIGKQSLWSAISNSNLHLGTVTEDGKSLCLQIIESS 502

Query: 481 NSSKIVTSSCICTNGDPTCLQDTQSQWFELVGTNTL 516
           NSSKIVT+SCICT  DPTCLQDTQSQWFELV TNTL
Sbjct: 503 NSSKIVTNSCICTTNDPTCLQDTQSQWFELVATNTL 538

[Close]

BLAST of Lsi08G003860 vs. ExPASy TrEMBL
Match: A0A5A7TMC9 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002090 PE=3 SV=1)

HSP 1 Score: 964.1 bits (2491), Expect = 2.3e-277
Identity = 463/514 (90.08%), Postives = 489/514 (95.14%), Query Frame = 0


Query: 2   SLPLSTQGRWIIDSQTGRRVKLVCVNWPSHTQSMLAEGLNHRSLKDLADEAIKLRFNCVR 61
           SLPLST+GRWIIDSQTGRRVKLVC+NWPSHTQSML EGLNHR LKDLADEAIKLRFNCVR
Sbjct: 23  SLPLSTRGRWIIDSQTGRRVKLVCMNWPSHTQSMLIEGLNHRPLKDLADEAIKLRFNCVR 82

Query: 62  LTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFVLNKTIPEAYEAVVDVLGASGL 121
           LTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFVLNKTI EAYEAVVDVLGASGL
Sbjct: 83  LTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFVLNKTIAEAYEAVVDVLGASGL 142

Query: 122 MVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLSLVAQRFNNKSTVVGMSLRNEI 181
           MVIADNH+SQPRWCCSLDDGNGFFGN  FDPQEWLQGLSLVAQRF NKSTVVGMSLRNEI
Sbjct: 143 MVIADNHISQPRWCCSLDDGNGFFGNNNFDPQEWLQGLSLVAQRFRNKSTVVGMSLRNEI 202

Query: 182 RGMMENANDWNNYITQGVTTIHNINSAVLVIVSGLNYDNDLRCLKEKPLTVKTLDNKLVF 241
           RG MENANDWN YITQGVTTIHNINS VLVIVSGLNYDNDLRCLKEKPL V TLDNKLVF
Sbjct: 203 RGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYDNDLRCLKEKPLNVGTLDNKLVF 262

Query: 242 EVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEFVIEGSNPFPLFVSEYGYDQREVD 301
           EVHLYSFSGDSESKFV+QPLN+ICANIMNGFIDHA FV++GSNPFPLFVSE+GYDQREV+
Sbjct: 263 EVHLYSFSGDSESKFVKQPLNDICANIMNGFIDHAGFVMQGSNPFPLFVSEFGYDQREVN 322

Query: 302 DAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAESTETFGVLEANWTQIKNPNFVK 361
           DAENRFMSCFTAHLA +DLDWALW WQGSYY+REGQAE  E+FGVL++NWTQ+KNPNF K
Sbjct: 323 DAENRFMSCFTAHLALRDLDWALWAWQGSYYFREGQAEPGESFGVLDSNWTQVKNPNFAK 382

Query: 362 KFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQVSNDNKEIFLTNCSTSTRWSHDNDSTPI 421
           KFQLLQTMLQDPNSN SFSYVIYHPQS QCIQVSNDNKEIFLT+CST TRWSH ND+TPI
Sbjct: 383 KFQLLQTMLQDPNSNESFSYVIYHPQSSQCIQVSNDNKEIFLTDCSTPTRWSHINDNTPI 442

Query: 422 TMSNTGLCLKASGEGLEASLSTDCSSKQSVWSAISSSKLHLATLTEDGKSLCLQIESSNS 481
            MS+TGLCLKASGEGLEASLSTDC S+QSVWSAIS+SKLHLAT+T+ GKSLCLQI+SSNS
Sbjct: 443 KMSSTGLCLKASGEGLEASLSTDCLSQQSVWSAISNSKLHLATVTQGGKSLCLQIDSSNS 502

Query: 482 SKIVTSSCICTNGDPTCLQDTQSQWFELVGTNTL 516
           SK+VT+SCICTNGDP CLQDT+SQWFELVGTNTL
Sbjct: 503 SKVVTNSCICTNGDPNCLQDTRSQWFELVGTNTL 536

[Close]

BLAST of Lsi08G003860 vs. ExPASy TrEMBL
Match: A0A5A7TMX0 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002070 PE=3 SV=1)

HSP 1 Score: 956.8 bits (2472), Expect = 3.6e-275
Identity = 461/515 (89.51%), Postives = 488/515 (94.76%), Query Frame = 0


Query: 2   SLPLSTQGRWIIDSQTGRRVKLVCVNWPSHTQSMLAEGLNHRSLKDLADEAIKLRFNCVR 61
           SLPLST+GRWIIDSQTGRRVKLVC+NWPSHTQSML EGLNHR LKDLADEAIKLRFNCVR
Sbjct: 26  SLPLSTRGRWIIDSQTGRRVKLVCMNWPSHTQSMLIEGLNHRPLKDLADEAIKLRFNCVR 85

Query: 62  LTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFVLNKTIPEAYEAVVDVLGASGL 121
           LTYATHMFTRYANRTIEENFDLLDLKQAKAGLA YNPFVLNKTI EAYEAVVD+LGASGL
Sbjct: 86  LTYATHMFTRYANRTIEENFDLLDLKQAKAGLALYNPFVLNKTIAEAYEAVVDILGASGL 145

Query: 122 MVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLSLVAQRFNNKSTVVGMSLRNEI 181
           MVIADNH+SQPRWCCSLDDGNGFFGN  FDPQEWLQGLSLVAQRF NKSTVVGMSLRNEI
Sbjct: 146 MVIADNHISQPRWCCSLDDGNGFFGNNNFDPQEWLQGLSLVAQRFRNKSTVVGMSLRNEI 205

Query: 182 RGMMENANDWNNYITQGVTTIHNINSAVLVIVSGLNYDNDLRCLKEKPLTVKTLDNKLVF 241
           RG MENANDWN YITQGVTTIHNINS VLVIVSGLNYDNDLRCLKEKPL V TLDNKLVF
Sbjct: 206 RGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYDNDLRCLKEKPLNVGTLDNKLVF 265

Query: 242 EVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEFVIEGSNPFPLFVSEYGYDQREVD 301
           EVHLYSFSGDSESKFV+QPLN+ICANIMNGFIDHA FV++GSNPFPLFVSE+GYDQREV+
Sbjct: 266 EVHLYSFSGDSESKFVKQPLNDICANIMNGFIDHAGFVMQGSNPFPLFVSEFGYDQREVN 325

Query: 302 DAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAESTETFGVLEANWTQIKNPNFVK 361
           DAENRFMSCFTAHLA +DLDWALW WQGSYY+REGQAE  E+FGVL++NWTQ+KNPNF K
Sbjct: 326 DAENRFMSCFTAHLALRDLDWALWAWQGSYYFREGQAEPGESFGVLDSNWTQVKNPNFAK 385

Query: 362 KFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQVSNDNKEIFLTNC-STSTRWSHDNDSTP 421
           KFQLLQTMLQDPNSNASFSYVIYHPQS QCIQVSNDNKEIFLT+C ST TRWSH ND+TP
Sbjct: 386 KFQLLQTMLQDPNSNASFSYVIYHPQSSQCIQVSNDNKEIFLTDCSSTPTRWSHINDNTP 445

Query: 422 ITMSNTGLCLKASGEGLEASLSTDCSSKQSVWSAISSSKLHLATLTEDGKSLCLQIESSN 481
           I MS+TGLCLKASGEGLEASLSTDC S+QSVWSAIS+SKLHLAT+T+ GKSLCLQI+SSN
Sbjct: 446 IKMSSTGLCLKASGEGLEASLSTDCLSQQSVWSAISNSKLHLATITQGGKSLCLQIDSSN 505

Query: 482 SSKIVTSSCICTNGDPTCLQDTQSQWFELVGTNTL 516
            SK+VT+SCICTNGDP CLQDT+SQWFELVGTNTL
Sbjct: 506 PSKVVTNSCICTNGDPNCLQDTRSQWFELVGTNTL 540

[Close]

BLAST of Lsi08G003860 vs. ExPASy TrEMBL
Match: A0A5A7THG1 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002110 PE=3 SV=1)

HSP 1 Score: 955.7 bits (2469), Expect = 8.0e-275
Identity = 458/514 (89.11%), Postives = 485/514 (94.36%), Query Frame = 0


Query: 2   SLPLSTQGRWIIDSQTGRRVKLVCVNWPSHTQSMLAEGLNHRSLKDLADEAIKLRFNCVR 61
           SLPLST+GRWIID QTGRRVKLVC+NWPSHTQSML EGLNHR LKDLADEAIKLRFNCVR
Sbjct: 23  SLPLSTRGRWIIDLQTGRRVKLVCMNWPSHTQSMLIEGLNHRPLKDLADEAIKLRFNCVR 82

Query: 62  LTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFVLNKTIPEAYEAVVDVLGASGL 121
           LTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFVLNKTI EAYEAVVD+LGASGL
Sbjct: 83  LTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFVLNKTIAEAYEAVVDILGASGL 142

Query: 122 MVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLSLVAQRFNNKSTVVGMSLRNEI 181
           MVIADNH+SQPRWCCSLDDGNGFFGN  FDPQEWLQGLSLVAQRF NKSTVVGMSLRNEI
Sbjct: 143 MVIADNHISQPRWCCSLDDGNGFFGNNNFDPQEWLQGLSLVAQRFRNKSTVVGMSLRNEI 202

Query: 182 RGMMENANDWNNYITQGVTTIHNINSAVLVIVSGLNYDNDLRCLKEKPLTVKTLDNKLVF 241
           RG MENANDWN YITQGVTTIHNINS VLVIVSGLNYDNDLRCLKEKPL V TLDNKLVF
Sbjct: 203 RGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYDNDLRCLKEKPLNVGTLDNKLVF 262

Query: 242 EVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEFVIEGSNPFPLFVSEYGYDQREVD 301
           EVHLYSFSGDSESKFV+QPLNNICANIMNGFIDHA FV++GSNPFPLFVSE+GYDQREV+
Sbjct: 263 EVHLYSFSGDSESKFVKQPLNNICANIMNGFIDHAGFVMQGSNPFPLFVSEFGYDQREVN 322

Query: 302 DAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAESTETFGVLEANWTQIKNPNFVK 361
           DAENRFMSCFTAHLA +DLDWALW WQGSYY+REGQAE  E+FGVL++NWTQ+KNPNF K
Sbjct: 323 DAENRFMSCFTAHLALRDLDWALWAWQGSYYFREGQAEPGESFGVLDSNWTQVKNPNFAK 382

Query: 362 KFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQVSNDNKEIFLTNCSTSTRWSHDNDSTPI 421
           KFQLLQTMLQDPNSNASFSYVIYHPQ+ QCIQVSNDNKEIFLT+CST TRWSH ND+TPI
Sbjct: 383 KFQLLQTMLQDPNSNASFSYVIYHPQTSQCIQVSNDNKEIFLTDCSTPTRWSHINDNTPI 442

Query: 422 TMSNTGLCLKASGEGLEASLSTDCSSKQSVWSAISSSKLHLATLTEDGKSLCLQIESSNS 481
            MS+TGLCLKASGEGLEASLSTDC  +QSVWSAIS+SKLHLAT+ + G SLCLQI+SSNS
Sbjct: 443 KMSSTGLCLKASGEGLEASLSTDCLIQQSVWSAISNSKLHLATVAQGGNSLCLQIDSSNS 502

Query: 482 SKIVTSSCICTNGDPTCLQDTQSQWFELVGTNTL 516
           SK+VT+SCICTNGDP CLQDT+SQWFELV TNTL
Sbjct: 503 SKVVTNSCICTNGDPNCLQDTRSQWFELVATNTL 536

[Close]

BLAST of Lsi08G003860 vs. ExPASy TrEMBL
Match: A0A6J1JZU8 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111491191 PE=3 SV=1)

HSP 1 Score: 943.0 bits (2436), Expect = 5.4e-271
Identity = 451/515 (87.57%), Postives = 480/515 (93.20%), Query Frame = 0


Query: 1   YSLPLSTQGRWIIDSQTGRRVKLVCVNWPSHTQSMLAEGLNHRSLKDLADEAIKLRFNCV 60
           YSLPLST+ +WIIDS TGRRVKLVCVNWPSHTQSML EGLNHR LK+LADEAIKLRFNCV
Sbjct: 22  YSLPLSTKEKWIIDSTTGRRVKLVCVNWPSHTQSMLIEGLNHRPLKELADEAIKLRFNCV 81

Query: 61  RLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFVLNKTIPEAYEAVVDVLGASG 120
           RLTYAT MFTRYANRT+EENFDLLDL+QAKAGLAQ NPFVLNKTI EAYEAVVDVLG SG
Sbjct: 82  RLTYATQMFTRYANRTVEENFDLLDLEQAKAGLAQNNPFVLNKTIAEAYEAVVDVLGESG 141

Query: 121 LMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLSLVAQRFNNKSTVVGMSLRNE 180
           LMVIADNH+SQPRWCCSLDDGNGFFG+RYFDPQEWLQGLSLVAQRF+ KSTVVGMSLRNE
Sbjct: 142 LMVIADNHISQPRWCCSLDDGNGFFGDRYFDPQEWLQGLSLVAQRFSKKSTVVGMSLRNE 201

Query: 181 IRGMMENANDWNNYITQGVTTIHNINSAVLVIVSGLNYDNDLRCLKEKPLTVKTLDNKLV 240
           IRG  ENANDWNNY+TQGVTTIHNIN  VLVIVSGLNYDNDLRCLKEKPL V TLDNKLV
Sbjct: 202 IRGTNENANDWNNYVTQGVTTIHNINPNVLVIVSGLNYDNDLRCLKEKPLNVSTLDNKLV 261

Query: 241 FEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEFVIEGSNPFPLFVSEYGYDQREV 300
           FEVHLYSFSGD ESKF+ QPLNNICANI+NGF+DHAEFV EGSNPFPLFVSEYGYDQREV
Sbjct: 262 FEVHLYSFSGDPESKFINQPLNNICANIINGFVDHAEFVTEGSNPFPLFVSEYGYDQREV 321

Query: 301 DDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAESTETFGVLEANWTQIKNPNFV 360
           +DAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAE  ETFGVL++NWTQIKNPNFV
Sbjct: 322 NDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAEPGETFGVLDSNWTQIKNPNFV 381

Query: 361 KKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQVSNDNKEIFLTNCSTSTRWSHDNDSTP 420
           +KFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQVSNDNK+IF+ NCS S+RW+HDNDSTP
Sbjct: 382 QKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQVSNDNKDIFMGNCSISSRWTHDNDSTP 441

Query: 421 ITMSNTGLCLKASGEGLEASLSTDCSSKQSVWSAISSSKLHLATLTEDGKSLCLQIESSN 480
           I MS+ GLCLK  GEGL  SLSTDC   QS WSAIS++KLHLAT+++DGKSLCLQ+ESSN
Sbjct: 442 IRMSSMGLCLKTIGEGLTPSLSTDCLGPQSSWSAISNTKLHLATISQDGKSLCLQVESSN 501

Query: 481 SSKIVTSSCICTNGDPTCLQDTQSQWFELVGTNTL 516
           SSKIVT+SCICTNG P CLQDT+SQWFELV TNTL
Sbjct: 502 SSKIVTNSCICTNGAPNCLQDTRSQWFELVKTNTL 536

[Close]

BLAST of Lsi08G003860 vs. NCBI nr
Match: KAE8646606.1 (hypothetical protein Csa_005432 [Cucumis sativus])

HSP 1 Score: 978.0 bits (2527), Expect = 3.1e-281
Identity = 472/516 (91.47%), Postives = 491/516 (95.16%), Query Frame = 0


Query: 1   YSLPLSTQGRWIIDSQTGRRVKLVCVNWPSHTQSMLAEGLNHRSLKDLADEAIKLRFNCV 60
           YSLPLST GRWIIDSQ+G+RVKLVCVNWPSHTQSML EGLNHR LK+LADEAIKLRFNCV
Sbjct: 51  YSLPLSTHGRWIIDSQSGKRVKLVCVNWPSHTQSMLIEGLNHRPLKELADEAIKLRFNCV 110

Query: 61  RLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFVLNKTIPEAYEAVVDVLGASG 120
           RLTYATHMFTRYANRT+EENFDLLDL+QAKAGLAQYNPFVLNKTI EAYEAVVDVLGASG
Sbjct: 111 RLTYATHMFTRYANRTVEENFDLLDLEQAKAGLAQYNPFVLNKTIAEAYEAVVDVLGASG 170

Query: 121 LMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLSLVAQRFNNKSTVVGMSLRNE 180
           LMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLSLVAQRFNNKSTVVGMSLRNE
Sbjct: 171 LMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLSLVAQRFNNKSTVVGMSLRNE 230

Query: 181 IRGMMENANDWNNYITQGVTTIHNINSAVLVIVSGLNYDNDLRCLKEKPLTVKTLDNKLV 240
           +RGMMENANDWNNY+TQGVTTIH IN AVLVIVSGLNYDNDLRCLK+KPL V TLDNKL 
Sbjct: 231 LRGMMENANDWNNYVTQGVTTIHKINPAVLVIVSGLNYDNDLRCLKDKPLNVSTLDNKLA 290

Query: 241 FEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEFVIEGSNPFPLFVSEYGYDQREV 300
           FEVHLYSFSGDSESKFVQQPLNNICA IM+ FIDHAEFVIEG NPFPLFVSEYGYDQREV
Sbjct: 291 FEVHLYSFSGDSESKFVQQPLNNICAKIMHEFIDHAEFVIEGPNPFPLFVSEYGYDQREV 350

Query: 301 DDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAESTETFGVLEANWTQIKNPNFV 360
           DDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAE  ETFGVL++NWTQIKNPNFV
Sbjct: 351 DDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAELAETFGVLDSNWTQIKNPNFV 410

Query: 361 KKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQVSNDNKEIFLTNCSTSTRWSHDNDSTP 420
           +KFQLLQTMLQDP SNASFSYVIYH QSGQCI+VSNDNKEIFLTNCSTS+RWSHDNDSTP
Sbjct: 411 QKFQLLQTMLQDPYSNASFSYVIYHVQSGQCIEVSNDNKEIFLTNCSTSSRWSHDNDSTP 470

Query: 421 ITMSNTGLCLKASGEGLEASLSTDCSSKQSVWSAISSSKLHLATLTEDGKSLCLQ-IESS 480
           I MS+TGLCLKASGEGLEASLSTDC  KQS+WSAIS+S LHL T+TEDGKSLCLQ IESS
Sbjct: 471 IKMSSTGLCLKASGEGLEASLSTDCIGKQSLWSAISNSNLHLGTVTEDGKSLCLQIIESS 530

Query: 481 NSSKIVTSSCICTNGDPTCLQDTQSQWFELVGTNTL 516
           NSSKIVT+SCICT  DPTCLQDTQSQWFELV TNTL
Sbjct: 531 NSSKIVTNSCICTTNDPTCLQDTQSQWFELVATNTL 566

[Close]

BLAST of Lsi08G003860 vs. NCBI nr
Match: XP_011658389.1 (glycosyl hydrolase 5 family protein [Cucumis sativus])

HSP 1 Score: 978.0 bits (2527), Expect = 3.1e-281
Identity = 472/516 (91.47%), Postives = 491/516 (95.16%), Query Frame = 0


Query: 1   YSLPLSTQGRWIIDSQTGRRVKLVCVNWPSHTQSMLAEGLNHRSLKDLADEAIKLRFNCV 60
           YSLPLST GRWIIDSQ+G+RVKLVCVNWPSHTQSML EGLNHR LK+LADEAIKLRFNCV
Sbjct: 23  YSLPLSTHGRWIIDSQSGKRVKLVCVNWPSHTQSMLIEGLNHRPLKELADEAIKLRFNCV 82

Query: 61  RLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFVLNKTIPEAYEAVVDVLGASG 120
           RLTYATHMFTRYANRT+EENFDLLDL+QAKAGLAQYNPFVLNKTI EAYEAVVDVLGASG
Sbjct: 83  RLTYATHMFTRYANRTVEENFDLLDLEQAKAGLAQYNPFVLNKTIAEAYEAVVDVLGASG 142

Query: 121 LMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLSLVAQRFNNKSTVVGMSLRNE 180
           LMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLSLVAQRFNNKSTVVGMSLRNE
Sbjct: 143 LMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLSLVAQRFNNKSTVVGMSLRNE 202

Query: 181 IRGMMENANDWNNYITQGVTTIHNINSAVLVIVSGLNYDNDLRCLKEKPLTVKTLDNKLV 240
           +RGMMENANDWNNY+TQGVTTIH IN AVLVIVSGLNYDNDLRCLK+KPL V TLDNKL 
Sbjct: 203 LRGMMENANDWNNYVTQGVTTIHKINPAVLVIVSGLNYDNDLRCLKDKPLNVSTLDNKLA 262

Query: 241 FEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEFVIEGSNPFPLFVSEYGYDQREV 300
           FEVHLYSFSGDSESKFVQQPLNNICA IM+ FIDHAEFVIEG NPFPLFVSEYGYDQREV
Sbjct: 263 FEVHLYSFSGDSESKFVQQPLNNICAKIMHEFIDHAEFVIEGPNPFPLFVSEYGYDQREV 322

Query: 301 DDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAESTETFGVLEANWTQIKNPNFV 360
           DDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAE  ETFGVL++NWTQIKNPNFV
Sbjct: 323 DDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAELAETFGVLDSNWTQIKNPNFV 382

Query: 361 KKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQVSNDNKEIFLTNCSTSTRWSHDNDSTP 420
           +KFQLLQTMLQDP SNASFSYVIYH QSGQCI+VSNDNKEIFLTNCSTS+RWSHDNDSTP
Sbjct: 383 QKFQLLQTMLQDPYSNASFSYVIYHVQSGQCIEVSNDNKEIFLTNCSTSSRWSHDNDSTP 442

Query: 421 ITMSNTGLCLKASGEGLEASLSTDCSSKQSVWSAISSSKLHLATLTEDGKSLCLQ-IESS 480
           I MS+TGLCLKASGEGLEASLSTDC  KQS+WSAIS+S LHL T+TEDGKSLCLQ IESS
Sbjct: 443 IKMSSTGLCLKASGEGLEASLSTDCIGKQSLWSAISNSNLHLGTVTEDGKSLCLQIIESS 502

Query: 481 NSSKIVTSSCICTNGDPTCLQDTQSQWFELVGTNTL 516
           NSSKIVT+SCICT  DPTCLQDTQSQWFELV TNTL
Sbjct: 503 NSSKIVTNSCICTTNDPTCLQDTQSQWFELVATNTL 538

[Close]

BLAST of Lsi08G003860 vs. NCBI nr
Match: KAA0042711.1 (major extracellular endoglucanase-like [Cucumis melo var. makuwa])

HSP 1 Score: 964.1 bits (2491), Expect = 4.7e-277
Identity = 463/514 (90.08%), Postives = 489/514 (95.14%), Query Frame = 0


Query: 2   SLPLSTQGRWIIDSQTGRRVKLVCVNWPSHTQSMLAEGLNHRSLKDLADEAIKLRFNCVR 61
           SLPLST+GRWIIDSQTGRRVKLVC+NWPSHTQSML EGLNHR LKDLADEAIKLRFNCVR
Sbjct: 23  SLPLSTRGRWIIDSQTGRRVKLVCMNWPSHTQSMLIEGLNHRPLKDLADEAIKLRFNCVR 82

Query: 62  LTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFVLNKTIPEAYEAVVDVLGASGL 121
           LTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFVLNKTI EAYEAVVDVLGASGL
Sbjct: 83  LTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFVLNKTIAEAYEAVVDVLGASGL 142

Query: 122 MVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLSLVAQRFNNKSTVVGMSLRNEI 181
           MVIADNH+SQPRWCCSLDDGNGFFGN  FDPQEWLQGLSLVAQRF NKSTVVGMSLRNEI
Sbjct: 143 MVIADNHISQPRWCCSLDDGNGFFGNNNFDPQEWLQGLSLVAQRFRNKSTVVGMSLRNEI 202

Query: 182 RGMMENANDWNNYITQGVTTIHNINSAVLVIVSGLNYDNDLRCLKEKPLTVKTLDNKLVF 241
           RG MENANDWN YITQGVTTIHNINS VLVIVSGLNYDNDLRCLKEKPL V TLDNKLVF
Sbjct: 203 RGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYDNDLRCLKEKPLNVGTLDNKLVF 262

Query: 242 EVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEFVIEGSNPFPLFVSEYGYDQREVD 301
           EVHLYSFSGDSESKFV+QPLN+ICANIMNGFIDHA FV++GSNPFPLFVSE+GYDQREV+
Sbjct: 263 EVHLYSFSGDSESKFVKQPLNDICANIMNGFIDHAGFVMQGSNPFPLFVSEFGYDQREVN 322

Query: 302 DAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAESTETFGVLEANWTQIKNPNFVK 361
           DAENRFMSCFTAHLA +DLDWALW WQGSYY+REGQAE  E+FGVL++NWTQ+KNPNF K
Sbjct: 323 DAENRFMSCFTAHLALRDLDWALWAWQGSYYFREGQAEPGESFGVLDSNWTQVKNPNFAK 382

Query: 362 KFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQVSNDNKEIFLTNCSTSTRWSHDNDSTPI 421
           KFQLLQTMLQDPNSN SFSYVIYHPQS QCIQVSNDNKEIFLT+CST TRWSH ND+TPI
Sbjct: 383 KFQLLQTMLQDPNSNESFSYVIYHPQSSQCIQVSNDNKEIFLTDCSTPTRWSHINDNTPI 442

Query: 422 TMSNTGLCLKASGEGLEASLSTDCSSKQSVWSAISSSKLHLATLTEDGKSLCLQIESSNS 481
            MS+TGLCLKASGEGLEASLSTDC S+QSVWSAIS+SKLHLAT+T+ GKSLCLQI+SSNS
Sbjct: 443 KMSSTGLCLKASGEGLEASLSTDCLSQQSVWSAISNSKLHLATVTQGGKSLCLQIDSSNS 502

Query: 482 SKIVTSSCICTNGDPTCLQDTQSQWFELVGTNTL 516
           SK+VT+SCICTNGDP CLQDT+SQWFELVGTNTL
Sbjct: 503 SKVVTNSCICTNGDPNCLQDTRSQWFELVGTNTL 536

[Close]

BLAST of Lsi08G003860 vs. NCBI nr
Match: KAA0042709.1 (major extracellular endoglucanase-like [Cucumis melo var. makuwa])

HSP 1 Score: 956.8 bits (2472), Expect = 7.4e-275
Identity = 461/515 (89.51%), Postives = 488/515 (94.76%), Query Frame = 0


Query: 2   SLPLSTQGRWIIDSQTGRRVKLVCVNWPSHTQSMLAEGLNHRSLKDLADEAIKLRFNCVR 61
           SLPLST+GRWIIDSQTGRRVKLVC+NWPSHTQSML EGLNHR LKDLADEAIKLRFNCVR
Sbjct: 26  SLPLSTRGRWIIDSQTGRRVKLVCMNWPSHTQSMLIEGLNHRPLKDLADEAIKLRFNCVR 85

Query: 62  LTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFVLNKTIPEAYEAVVDVLGASGL 121
           LTYATHMFTRYANRTIEENFDLLDLKQAKAGLA YNPFVLNKTI EAYEAVVD+LGASGL
Sbjct: 86  LTYATHMFTRYANRTIEENFDLLDLKQAKAGLALYNPFVLNKTIAEAYEAVVDILGASGL 145

Query: 122 MVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLSLVAQRFNNKSTVVGMSLRNEI 181
           MVIADNH+SQPRWCCSLDDGNGFFGN  FDPQEWLQGLSLVAQRF NKSTVVGMSLRNEI
Sbjct: 146 MVIADNHISQPRWCCSLDDGNGFFGNNNFDPQEWLQGLSLVAQRFRNKSTVVGMSLRNEI 205

Query: 182 RGMMENANDWNNYITQGVTTIHNINSAVLVIVSGLNYDNDLRCLKEKPLTVKTLDNKLVF 241
           RG MENANDWN YITQGVTTIHNINS VLVIVSGLNYDNDLRCLKEKPL V TLDNKLVF
Sbjct: 206 RGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYDNDLRCLKEKPLNVGTLDNKLVF 265

Query: 242 EVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEFVIEGSNPFPLFVSEYGYDQREVD 301
           EVHLYSFSGDSESKFV+QPLN+ICANIMNGFIDHA FV++GSNPFPLFVSE+GYDQREV+
Sbjct: 266 EVHLYSFSGDSESKFVKQPLNDICANIMNGFIDHAGFVMQGSNPFPLFVSEFGYDQREVN 325

Query: 302 DAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAESTETFGVLEANWTQIKNPNFVK 361
           DAENRFMSCFTAHLA +DLDWALW WQGSYY+REGQAE  E+FGVL++NWTQ+KNPNF K
Sbjct: 326 DAENRFMSCFTAHLALRDLDWALWAWQGSYYFREGQAEPGESFGVLDSNWTQVKNPNFAK 385

Query: 362 KFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQVSNDNKEIFLTNC-STSTRWSHDNDSTP 421
           KFQLLQTMLQDPNSNASFSYVIYHPQS QCIQVSNDNKEIFLT+C ST TRWSH ND+TP
Sbjct: 386 KFQLLQTMLQDPNSNASFSYVIYHPQSSQCIQVSNDNKEIFLTDCSSTPTRWSHINDNTP 445

Query: 422 ITMSNTGLCLKASGEGLEASLSTDCSSKQSVWSAISSSKLHLATLTEDGKSLCLQIESSN 481
           I MS+TGLCLKASGEGLEASLSTDC S+QSVWSAIS+SKLHLAT+T+ GKSLCLQI+SSN
Sbjct: 446 IKMSSTGLCLKASGEGLEASLSTDCLSQQSVWSAISNSKLHLATITQGGKSLCLQIDSSN 505

Query: 482 SSKIVTSSCICTNGDPTCLQDTQSQWFELVGTNTL 516
            SK+VT+SCICTNGDP CLQDT+SQWFELVGTNTL
Sbjct: 506 PSKVVTNSCICTNGDPNCLQDTRSQWFELVGTNTL 540

[Close]

BLAST of Lsi08G003860 vs. NCBI nr
Match: KAA0042713.1 (major extracellular endoglucanase-like [Cucumis melo var. makuwa])

HSP 1 Score: 955.7 bits (2469), Expect = 1.7e-274
Identity = 458/514 (89.11%), Postives = 485/514 (94.36%), Query Frame = 0


Query: 2   SLPLSTQGRWIIDSQTGRRVKLVCVNWPSHTQSMLAEGLNHRSLKDLADEAIKLRFNCVR 61
           SLPLST+GRWIID QTGRRVKLVC+NWPSHTQSML EGLNHR LKDLADEAIKLRFNCVR
Sbjct: 23  SLPLSTRGRWIIDLQTGRRVKLVCMNWPSHTQSMLIEGLNHRPLKDLADEAIKLRFNCVR 82

Query: 62  LTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFVLNKTIPEAYEAVVDVLGASGL 121
           LTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFVLNKTI EAYEAVVD+LGASGL
Sbjct: 83  LTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFVLNKTIAEAYEAVVDILGASGL 142

Query: 122 MVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLSLVAQRFNNKSTVVGMSLRNEI 181
           MVIADNH+SQPRWCCSLDDGNGFFGN  FDPQEWLQGLSLVAQRF NKSTVVGMSLRNEI
Sbjct: 143 MVIADNHISQPRWCCSLDDGNGFFGNNNFDPQEWLQGLSLVAQRFRNKSTVVGMSLRNEI 202

Query: 182 RGMMENANDWNNYITQGVTTIHNINSAVLVIVSGLNYDNDLRCLKEKPLTVKTLDNKLVF 241
           RG MENANDWN YITQGVTTIHNINS VLVIVSGLNYDNDLRCLKEKPL V TLDNKLVF
Sbjct: 203 RGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYDNDLRCLKEKPLNVGTLDNKLVF 262

Query: 242 EVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEFVIEGSNPFPLFVSEYGYDQREVD 301
           EVHLYSFSGDSESKFV+QPLNNICANIMNGFIDHA FV++GSNPFPLFVSE+GYDQREV+
Sbjct: 263 EVHLYSFSGDSESKFVKQPLNNICANIMNGFIDHAGFVMQGSNPFPLFVSEFGYDQREVN 322

Query: 302 DAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAESTETFGVLEANWTQIKNPNFVK 361
           DAENRFMSCFTAHLA +DLDWALW WQGSYY+REGQAE  E+FGVL++NWTQ+KNPNF K
Sbjct: 323 DAENRFMSCFTAHLALRDLDWALWAWQGSYYFREGQAEPGESFGVLDSNWTQVKNPNFAK 382

Query: 362 KFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQVSNDNKEIFLTNCSTSTRWSHDNDSTPI 421
           KFQLLQTMLQDPNSNASFSYVIYHPQ+ QCIQVSNDNKEIFLT+CST TRWSH ND+TPI
Sbjct: 383 KFQLLQTMLQDPNSNASFSYVIYHPQTSQCIQVSNDNKEIFLTDCSTPTRWSHINDNTPI 442

Query: 422 TMSNTGLCLKASGEGLEASLSTDCSSKQSVWSAISSSKLHLATLTEDGKSLCLQIESSNS 481
            MS+TGLCLKASGEGLEASLSTDC  +QSVWSAIS+SKLHLAT+ + G SLCLQI+SSNS
Sbjct: 443 KMSSTGLCLKASGEGLEASLSTDCLIQQSVWSAISNSKLHLATVAQGGNSLCLQIDSSNS 502

Query: 482 SKIVTSSCICTNGDPTCLQDTQSQWFELVGTNTL 516
           SK+VT+SCICTNGDP CLQDT+SQWFELV TNTL
Sbjct: 503 SKVVTNSCICTNGDPNCLQDTRSQWFELVATNTL 536

[Close]

BLAST of Lsi08G003860 vs. TAIR 10
Match: AT1G13130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 412.1 bits (1058), Expect = 6.4e-115
Identity = 207/514 (40.27%), Postives = 324/514 (63.04%), Query Frame = 0


Query: 2   SLPLSTQGRWIIDSQTGRRVKLVCVNWPSHTQSMLAEGLNHRSLKDLADEAIKLRFNCVR 61
           S PLST  RWI+D + G RVKLVC NWPSH Q ++AEGL+ + +  +A + +++ FNCVR
Sbjct: 32  SYPLSTSSRWIVD-ENGLRVKLVCANWPSHLQPVVAEGLSKQPVDAVAKKIVEMGFNCVR 91

Query: 62  LTYATHMFTRYA---NRTIEENFDLLDLKQAKAGLAQYNPFVLNKTIPEAYEAVVDVLGA 121
           LT+   + T      N T+ ++F  L L     G    NP +++  + EAY+ VV  LG 
Sbjct: 92  LTWPLDLMTNETLANNVTVRQSFQSLGLNDDIVGFQTNNPSIIDLPLIEAYKTVVTTLGN 151

Query: 122 SGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLSLVAQRFNNKSTVVGMSLR 181
           + +MVI DNH+++P WCC+ DDGNGFFG+++FDP  W+  L  +A  FN  S VVGMSLR
Sbjct: 152 NDVMVILDNHLTKPGWCCANDDGNGFFGDQFFDPTVWVAALKKMAATFNGVSNVVGMSLR 211

Query: 182 NEIRGMMENANDWNNYITQGVTTIHNINSAVLVIVSGLNYDNDLRCLKEKPLTVKTLDNK 241
           NE+RG  +N NDW  Y+ QG   +H+ N+ VLVI+SGL++D DL  ++ +P+ + +   K
Sbjct: 212 NELRGPKQNVNDWFKYMQQGAEAVHSANNKVLVILSGLSFDADLSFVRSRPVKL-SFTGK 271

Query: 242 LVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEFVIEGSNPFPLFVSEYGYDQR 301
           LVFE+H YSFS D  S     P N+IC  ++N   +   +++  +  FPLF+SE+G D+R
Sbjct: 272 LVFELHWYSFS-DGNSWAANNP-NDICGRVLNRIGNGGGYLL--NQGFPLFLSEFGIDER 331

Query: 302 EVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAESTETFGVLEANWTQIKNPN 361
            V+  +NR+  C T   A+ D+DW+LW   GSYY R+G+    E +GVL+++W  ++N +
Sbjct: 332 GVNTNDNRYFGCLTGWAAENDVDWSLWALTGSYYLRQGKVGMNEYYGVLDSDWISVRNSS 391

Query: 362 FVKKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQVS-NDNKEIFLTNCSTSTRWSHDND 421
           F++K   LQ+ LQ P        +++HP +G CI  S +D K + L  C++S  WS+   
Sbjct: 392 FLQKISFLQSPLQGPGPRTDAYNLVFHPLTGLCIVRSLDDPKMLTLGPCNSSEPWSYTKK 451

Query: 422 STPITMSNTGLCLKASGEGLEASLS-TDCSSKQSVWSAISSSKLHLATLTEDGKSLCLQI 481
           +  + + +  LCL+++G     +++ T CS+  S W  IS+S++HLA+ T +  SLCL +
Sbjct: 452 A--LRIKDQQLCLQSNGPKNPVTMTRTSCSTSGSKWQTISASRMHLASTTSNKTSLCLDV 511

Query: 482 ESSNSSKIVTSSCICTNGDPTCLQDTQSQWFELV 511
           +++N+  +V ++C C + D +C  +  SQWF+++
Sbjct: 512 DTANN--VVANACKCLSKDKSC--EPMSQWFKII 533

[Close]

BLAST of Lsi08G003860 vs. TAIR 10
Match: AT3G26140.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 384.4 bits (986), Expect = 1.4e-106
Identity = 203/514 (39.49%), Postives = 315/514 (61.28%), Query Frame = 0


Query: 4   PLSTQGRWIIDSQTGRRVKLVCVNWPSHTQSMLAEGLNHRSLKDLADEAIKLRFNCVRLT 63
           PLST  RWIID + G+RVKL CVNWPSH Q ++AEGL+ +S+ DLA + + + FNCVR T
Sbjct: 4   PLSTNSRWIID-EKGQRVKLACVNWPSHLQPVVAEGLSKQSVDDLAKKIMAMGFNCVRFT 63

Query: 64  YATHMFTRYA---NRTIEENFDLLDLKQAKAGLAQYNPFVLNKTIPEAYEAVVDVLGASG 123
           +   + T      N T+ ++F  L L    +G    NP +++  + EAY+ VV  LG + 
Sbjct: 64  WPLDLATNETLANNVTVRQSFQSLGLNDDISGFETKNPSMIDLPLIEAYKKVVAKLGNNN 123

Query: 124 LMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLSLVAQRFNNKSTVVGMSLRNE 183
           +MVI DNH+++P WCC  +DGNGFFG+ +FDP  W+ GL+ +A  F   + VVGMSLRNE
Sbjct: 124 VMVILDNHVTKPGWCCGYNDGNGFFGDTFFDPTTWIAGLTKIAMTFKGATNVVGMSLRNE 183

Query: 184 IRGMMENANDWNNYITQGVTTIHNINSAVLVIVSGLNYDNDLRCLKEKPLTVKTLDNKLV 243
           +RG  +N +DW  Y+ QG   +H  N  VLVI+SGL+YD DL  ++ + + + T   KLV
Sbjct: 184 LRGPKQNVDDWFKYMQQGAEAVHEANPNVLVILSGLSYDTDLSFVRSRHVNL-TFTRKLV 243

Query: 244 FEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEFVIEGSNPFPLFVSEYGYDQREV 303
           FE+H YSF+  + + +  +  N  C  I+    +   F +     FP+F+SE+G D R  
Sbjct: 244 FELHRYSFT--NTNTWSSKNPNEACGEILKSIENGGGFNL---RDFPVFLSEFGIDLRGK 303

Query: 304 DDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAESTETFGVLEANWTQIKNPNFV 363
           +  +NR++ C     A+ D+DW++WT QGSYY REG    +E +G+L+++W ++++ +F+
Sbjct: 304 NVNDNRYIGCILGWAAENDVDWSIWTLQGSYYLREGVVGMSEFYGILDSDWVRVRSQSFL 363

Query: 364 KKFQLLQTMLQDPNSNASFSYVIYHPQSGQC-IQVSNDNKEIFLTNCSTSTRWSHDNDST 423
           ++  L+ + LQ P S +    +++HP +G C +Q   D  ++ L  C+ S  WS+   +T
Sbjct: 364 QRLSLILSPLQGPGSQSKVYNLVFHPLTGLCMLQSILDPTKVTLGLCNESQPWSYTPQNT 423

Query: 424 PITMSNTGLCLKASGEGLEASLS-TDCSSKQ-SVWSAISSSKLHLATLTEDGKSLCLQIE 483
            +T+ +  LCL+++G      LS T CSS   S W  IS+S + LA  + +  SLCL ++
Sbjct: 424 -LTLKDKSLCLESTGPNAPVKLSETSCSSPNLSEWETISASNMLLAAKSTN-NSLCLDVD 483

Query: 484 SSNSSKIVTSSCICTNG-DPTCLQDTQSQWFELV 511
            +N+  ++ S+C C  G D +C  D  SQWF++V
Sbjct: 484 ETNN--LMASNCKCVKGEDSSC--DPISQWFKIV 504

[Close]

BLAST of Lsi08G003860 vs. TAIR 10
Match: AT3G26130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 380.9 bits (977), Expect = 1.6e-105
Identity = 212/521 (40.69%), Postives = 308/521 (59.12%), Query Frame = 0


Query: 1   YSLPLSTQGRWII-DSQTGRRVKLVCVNWPSHTQSMLAEGLNHRSLKDLADEAIKLRFNC 60
           ++ P ST  RWI+ D   GRRVKL CVNWPSH ++ +AEGL+ + L  +A++ + + FNC
Sbjct: 19  FAFPPSTDSRWIVDDGNKGRRVKLTCVNWPSHLETAVAEGLSKQPLDAIAEKIVSMGFNC 78

Query: 61  VRLTYATHMFTR---YANRTIEENFDLLDLKQAKAGLAQYNPFVLNKTIPEAYEAVVDVL 120
           VRLT+  ++ T     A  T+ ++     L +A +G   +NP +L+  + +A++ VV  L
Sbjct: 79  VRLTWPLYLATDESFSAFMTVRQSLRKFRLFEAVSGFQTHNPTILDLPLIKAFQEVVYCL 138

Query: 121 GASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLSLVAQRFNN-KSTVVGM 180
               +MVI DNH+SQP WCCS +DGNGFFG+++ +PQ W++GL  +A  F N  S VVGM
Sbjct: 139 EKHRVMVILDNHISQPGWCCSDNDGNGFFGDKHLNPQVWIKGLKKMASMFANVSSNVVGM 198

Query: 181 SLRNEIRGMMENANDWNNYITQGVTTIHNINSAVLVIVSGLNYDNDLRCLKEKPLTVKTL 240
           SLRNE+RG  +N  DW  Y+ +G   +H++N  VLVIVSGLNY  DL  L+E+P  V + 
Sbjct: 199 SLRNELRGPKQNIKDWYKYMREGAEAVHSVNPNVLVIVSGLNYATDLSFLRERPFEV-SF 258

Query: 241 DNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEFVIEGSNPFPLFVSEYGY 300
             K+VFE+H Y F    E       LN IC       +  + F++E     PLFVSE+G 
Sbjct: 259 RRKVVFEIHWYGFWNTWEG----DNLNKICGKETEKMMKMSGFLLE--KGIPLFVSEFGI 318

Query: 301 DQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAESTETFGVLEANWTQIK 360
           DQR  +  +N+F+SCF A  A +DLDW+LWT  GSYY RE    S E++GVL+ NW+ I+
Sbjct: 319 DQRGNNANDNKFLSCFMALAADRDLDWSLWTLAGSYYIREKSIGSDESYGVLDFNWSSIR 378

Query: 361 NPNFVKKFQLLQT-----MLQDPNSNASFSYVIYHPQSGQCIQVSNDNKEIFLTNCSTST 420
           N   ++    +QT     M   P        +++HP +G CI V     ++ L +C+ S 
Sbjct: 379 NSTILQMISAIQTPFIGLMETQPKK------IMFHPSTGLCI-VRKSLFQLKLGSCNRSE 438

Query: 421 RWSHDNDSTPITMSNTGLCLKASGEGLEASLSTDCS-SKQSVWSAISSSKLHLATLTEDG 480
            W   +           LCLKA  +G    L    S S  S W   S SK+ L+++T++G
Sbjct: 439 SWRLSSHRVLSLAEEQILCLKAYEKGKSVKLRLFFSESYCSKWKLFSDSKMQLSSITKNG 498

Query: 481 KSLCLQIESSNSSKIVTSSCICTNGDPTCLQDTQSQWFELV 511
            S+CL +++ N++ IVT+SC C  G+ +C  D +SQWF+LV
Sbjct: 499 FSVCLDVDTENNN-IVTNSCKCLRGNSSC--DPRSQWFKLV 522

[Close]

BLAST of Lsi08G003860 vs. TAIR 10
Match: AT5G17500.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 350.9 bits (899), Expect = 1.7e-96
Identity = 188/512 (36.72%), Postives = 292/512 (57.03%), Query Frame = 0


Query: 4   PLSTQGRWIIDSQTGRRVKLVCVNWPSHTQSMLAEGLNHRSLKDLADEAIKLRFNCVRLT 63
           PL T+ RWI++++ G RVKL C NWPSH + ++AEGL+ + +  ++ +   + FNCVRLT
Sbjct: 27  PLFTKSRWIVNNK-GHRVKLACANWPSHLKPVVAEGLSSQPMDSISKKIKDMGFNCVRLT 86

Query: 64  YATHMF---TRYANRTIEENFDLLDLKQAKAGLAQYNPFVLNKTIPEAYEAVVDVLGASG 123
           +   +    T   N T++++F+   L     G+  +NP+++N  +   ++AVV  LG   
Sbjct: 87  WPLELMINDTLAFNVTVKQSFERYGLDHELQGIYTHNPYIVNTPLINVFQAVVYSLGRHD 146

Query: 124 LMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLSLVAQRFNNKSTVVGMSLRNE 183
           +MVI DNH + P WCCS DD + FFG+  F+P  W+ GL  +A  F N   VVGMSLRNE
Sbjct: 147 VMVILDNHKTVPGWCCSNDDPDAFFGDPKFNPDLWMLGLKKMATIFMNVKNVVGMSLRNE 206

Query: 184 IRGMMENANDWNNYITQGVTTIHNINSAVLVIVSGLNYDNDLRCLKEKPLTVKTLDNKLV 243
           +RG    + DW  Y+ +G   +H  N  VLVI+SGLN+D DL  LK++P+ + +   KLV
Sbjct: 207 LRGYNHTSKDWYKYMQKGAEAVHTSNPNVLVILSGLNFDADLSFLKDRPVNL-SFKKKLV 266

Query: 244 FEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEFVIEGSNPFPLFVSEYGYDQREV 303
            E+H YSF+ D   ++    +N+ C+ + +       FV++    FPLF+SE+G DQR  
Sbjct: 267 LELHWYSFT-DGTGQWKSHNVNDFCSQMFSKERRTGGFVLD--QGFPLFLSEFGTDQRGG 326

Query: 304 DDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAESTETFGVLEANWTQIKNPNFV 363
           D   NR+M+C  A  A+KDLDWA+W   G YY+REG+    E +G+L+ANW  + N  ++
Sbjct: 327 DLEGNRYMNCMLAWAAEKDLDWAVWAVTGVYYFREGKRGVVEAYGMLDANWHNVHNYTYL 386

Query: 364 KKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQVSN--DNKEIFLTNCSTSTRWSHDNDS 423
           ++  ++Q     P    +    I+HP +G C+   +     E+ L  C+    WS+ +  
Sbjct: 387 RRLSVIQPPHTGPGVKHNHHKKIFHPLTGLCLVRKSHCHESELTLGPCTKDEPWSYSHGG 446

Query: 424 TPITMSNTGLCLKA-SGEGLEASLSTDCSSKQSVWSAISSSKLHLATLTEDGKSLCLQIE 483
                     CL+  +  G    L   C+  +     IS++K+HL+  T DG  +CL ++
Sbjct: 447 ILEIRRGHKSCLEGETAVGKSVKLGRICTKIEQ----ISATKMHLSFNTSDGSLVCLDVD 506

Query: 484 SSNSSKIVTSSCICTNGDPTCLQDTQSQWFEL 510
           S N+  +V +SC C  GD TC  +  SQWF++
Sbjct: 507 SDNN--VVANSCNCLTGDTTC--EPASQWFKI 525

[Close]

BLAST of Lsi08G003860 vs. TAIR 10
Match: AT5G16700.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 299.7 bits (766), Expect = 4.6e-81
Identity = 180/517 (34.82%), Postives = 280/517 (54.16%), Query Frame = 0


Query: 2   SLPLSTQGRWIIDSQTGRRVKLVCVNWPSHTQSMLAEGLNHRSLKDLADEAIKLRFNCVR 61
           S PLST+ RWI+D + G+RVKL CVNWP+H Q  +AEGL+ + L  ++ + + + FNCVR
Sbjct: 23  SYPLSTKSRWIVD-EKGQRVKLACVNWPAHLQPTVAEGLSKQPLDSISKKIVSMGFNCVR 82

Query: 62  LTYATHMFTR---YANRTIEENFDLLDLKQAKAGLAQYNPFVLNKTIPEAYEAVVDVLGA 121
           LT+   + T        T++++F+ L L +   G+  +NP +L+  +  A++ VV  LG 
Sbjct: 83  LTWPLDLVTNDTLALKVTVKQSFESLKLFEDVLGIQTHNPKLLHLPLFNAFQEVVSNLGE 142

Query: 122 SGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLSLVAQRFNNKSTVVGMSLR 181
           +G+MVI DNH++ P WCC  +D + FFG  +FDP  W +GL  +A  F N + V+GMSLR
Sbjct: 143 NGVMVILDNHLTTPGWCCGDNDLDAFFGYPHFDPLVWAKGLRKMATLFRNFTHVIGMSLR 202

Query: 182 NEIRGMMENANDWNNYITQGVTTIHNINSAVLVIVSGLNYDNDLRCLKEKPLTVKTLDNK 241
           NE RG  +  + W  ++ QG   +H  N  +LVI+SG+++D +L  L+++ + V   D K
Sbjct: 203 NEPRGARDYPDLWFRHMPQGAEAVHAANPKLLVILSGIDFDTNLSFLRDRSVNVSFTD-K 262

Query: 242 LVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAEFVIEGSNPFPLFVSEYGYDQR 301
           LVFE+H YSFS D    + +   N+ C  I+     +  F++     FPL +SE+G DQR
Sbjct: 263 LVFELHWYSFS-DGRDSWRKHNSNDFCVKIIEKVTHNGGFLL--GRGFPLILSEFGTDQR 322

Query: 302 EVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAESTETFGVLEANWTQIKNPN 361
             D + NR+M+C  A  A+ DLDWA+W   G YY R G                      
Sbjct: 323 GGDMSGNRYMNCLVAWAAENDLDWAVWALTGDYYLRTGPG-------------------- 382

Query: 362 FVKKFQLLQTMLQDPNSNASFSYVIYHPQSGQCI--QVSNDNKEIFLTNCSTSTRWSHDN 421
                         PN N     +++HP +G C+    S++   + L  C  S  W+  N
Sbjct: 383 ------------LRPNKN-----LLFHPSTGLCVTNNPSDNIPTLRLGPCPKSDPWTF-N 442

Query: 422 DSTPITMSNTGLCLKAS---GEGLEASLSTDCSSKQSVWSAISSSKLHLATLTEDGKSLC 481
            S  I   N  +C++A    G+ ++  + T CS        IS++K+HL+  T +G  LC
Sbjct: 443 PSEGILWINK-MCVEAPNVVGQKVKLGVGTKCSK----LGQISATKMHLSFKTSNGLLLC 488

Query: 482 LQIESSNSSKIVTSSCICTNGDPTCLQDTQSQWFELV 511
           L ++  ++S +V + C     D +C  D  SQWF+++
Sbjct: 503 LDVDERDNS-VVANRCKFLTMDASC--DPASQWFKVL 488

The following BLAST results are available for this feature:

BLAST of Lsi08G003860 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
C0HLA0	1.8e-114	41.86	Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1	[more]
P23548	3.6e-14	22.87	Endoglucanase OS=Paenibacillus polymyxa OX=1406 PE=3 SV=2	[more]
P19487	5.8e-12	24.48	Major extracellular endoglucanase OS=Xanthomonas campestris pv. campestris (stra...	[more]

BLAST of Lsi08G003860 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K853	1.5e-281	91.47	Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_6G028440 PE=3...	[more]
A0A5A7TMC9	2.3e-277	90.08	Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A5A7TMX0	3.6e-275	89.51	Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A5A7THG1	8.0e-275	89.11	Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A6J1JZU8	5.4e-271	87.57	Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111491191 PE=...	[more]

BLAST of Lsi08G003860 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8646606.1	3.1e-281	91.47	hypothetical protein Csa_005432 [Cucumis sativus]	[more]
XP_011658389.1	3.1e-281	91.47	glycosyl hydrolase 5 family protein [Cucumis sativus]	[more]
KAA0042711.1	4.7e-277	90.08	major extracellular endoglucanase-like [Cucumis melo var. makuwa]	[more]
KAA0042709.1	7.4e-275	89.51	major extracellular endoglucanase-like [Cucumis melo var. makuwa]	[more]
KAA0042713.1	1.7e-274	89.11	major extracellular endoglucanase-like [Cucumis melo var. makuwa]	[more]

BLAST of Lsi08G003860 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G13130.1	6.4e-115	40.27	Cellulase (glycosyl hydrolase family 5) protein	[more]
AT3G26140.1	1.4e-106	39.49	Cellulase (glycosyl hydrolase family 5) protein	[more]
AT3G26130.1	1.6e-105	40.69	Cellulase (glycosyl hydrolase family 5) protein	[more]
AT5G17500.1	1.7e-96	36.72	Glycosyl hydrolase superfamily protein	[more]
AT5G16700.1	4.6e-81	34.82	Glycosyl hydrolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	2.80.10.50		coord: 382..509 e-value: 3.8E-6 score: 29.1
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 3..372 e-value: 2.0E-65 score: 223.3
None	No IPR available	PANTHER	PTHR31263	CELLULASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G14560)	coord: 2..511
IPR001547	Glycoside hydrolase, family 5	PFAM	PF00150	Cellulase	coord: 41..328 e-value: 5.1E-25 score: 88.5
IPR000772	Ricin B, lectin domain	PROSITE	PS50231	RICIN_B_LECTIN	coord: 378..509 score: 8.76
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 4..363
IPR035992	Ricin B-like lectins	SUPERFAMILY	50370	Ricin B-like lectins	coord: 377..490

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi08G003860.1	Lsi08G003860.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071704	organic substance metabolic process
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0016985	mannan endo-1,4-beta-mannosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds