Lsi08G003330 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi08G003330
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionPectin lyase-like superfamily protein
Locationchr08: 9040404 .. 9042733 (+)
RNA-Seq ExpressionLsi08G003330
SyntenyLsi08G003330
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AACAAACTCTCCAAGAGATCAAAAAAGAATAAAAGGTATCTTAAAAAAAAAAAGAAAGAAGAAGAACAAGAATATAAGGTAGAAGTAGGAACAAGATGAGTATGAAGTTGAACTTGATGACTCTAACCTTCATACTATTATACGTTTCTATTAGGAAAGCCCAATCTAGTGATACTATTTTCGATGTGAGAAAGTACGGTGCTATACCTAATGCTGATATAACTCAGGTAAGAACCAAATGTTTAGACTCTATTTGATAATCATTTGATTTTTATTTTTAATTTTCAAAAATTAAGCTTACAAGCTCTAATTTCATCTATTGATTTCTTTGTTTCCTTATGTACTTTTAGAAGTGTTTTTAAGAGCTAAGCAATTTTTTTTCCTTTTTCTCTTTAAAAAAAATAATAAAGTCATTTTTAAGAATTTGTTTTTGTTATTGAAATTTTACTTAGAATTCAATTGTTTACTTAAGAACGATAAAGTTATGGTAAGAAAACGTGAAAAAACAATAACAACTTTTAAAAGAAAAAAAAATTAAATGGTTATAAAAATAGGGCCTTCAATATATTATTGTTTGAGTGGGAAAGAAAAATACTCTAAACTTGATAGATCGTGCCTGATTTATATTTGATATTTAAAATAGGCTCTAAAAAATGCATGGAAGGATGCCTGTGCATCAACGAGTCAAAGTACAATAATTATTCCAAACGTGGTTTACAAGTTAAGTGCAATTGAGTTAGTAGGACCATGTAAAGGTCCGATTGGAATTCAACTACAAGGGACATTGCAAGCTCCAGCAGATCTAACAGGTGAAGGTTGGGTTCATTTCAAATATATCGATCATTTTTCATTAACAGGTGGTGGAGTTTTTGACGGGCAAGGAAAACAAGCTTGGGAAAACAATGATTGTGATAAAAACCCTAGTTGTGCAAGAATTCCCATGGTAAGCACACTTAAATATCTAATTAACAATTTTAGTACATTTCTCATTTCTAGTGTATATATCAATCAAATTATAATCTCTATATCGTGATTAGTATTTTAATTATATCAAACTAAACATATTCTTTTTCTCAACTAAATATGTATGTTTATTTTATTATTATAACTAATAATATTTTTTTGGGAATTATTATTATAACTAATAATATTTTTTTGAGAAATATACAGAGTTTAAAGTTCAGCTTTATCACACATTCAATCGTGAGTGACATAACCTCTAAAGATAGCAAGAACTTTCATGTCCATGTATTGGGTTGTAATAACCTTACCCTACAACATGTAAATATAAATGCACCAGGAAATAGTATTAACACTGATGGGATTCACATTGGACGTTCTATTGGAATTAATATTATTAATACAAACATTGGTACTGGAGACGATTGTATTTCACTTGGAGACGGAAGCAAAGAAGTAACAATTACCAATGTGACATGTGGGCCTGGACATGGTATCAGTGTAGGAAGTTTAGGAAGGTATGTTGTTGAAGAACCAGTTGAGGGACTTCGAGTTAAGAATTGTACGTTAATGAATACATCAAATGGCTTGAGAATTAAAACATGGCCAGCTTCTCCCGCTGCTGGAACAGCTTCTGATATGCATTATGAAGATATAATTATGGTCAATGTCAGCAATCCCATTCTCGTAGATCAAGAATATTGTCCATACAACAAATGTAACAAAGAGGTACTAAAGTTATGAAAATTTTTACTTTTTACCCCTTTTGCATAAGTTGATGTATTTCAATCAAAACTCAAGTAACCATGGATTATTTCTTGCTTTTCAGATTCCATCTAAAGTCAAGATTAGTAAGGTTAGTTTCAAGAATATCAGAGGTACTTCTGGTAATGCACTCGCAGTAAAACTTGTTTGTAGTTCTAGTGTTCCATGTGAAGATGTTGAGCTAGCTGACATTGACCTCACTTACAATGGAAGTGAAGGCCCTATTTCATCTCAATGCTCAAATGTGAAGCCCACTGTTACTGGAAAGCAAAACCCACCTGCTTGTTCTTCCCTTGCTCTCGATTCTTCACCACCAGCTTAAACTATTCCTGCAAAGATGACCATTGGATACAAAAATTTTGAATGTTATTTTTTTTTACATAATTAGTGTTTAGTTTATATGATATAAGAGAATTTTGTAATTTCTCATTGTTTTGCCACTCAAGTTCTTATAGAAAAAACTTGGAGTGAATGTAGGCTTTTCCTCTTTTGAATTATCAAATGAAGATACATTTCTTCCTTAAATATTATTTTGGTTTATTAATTATACCCAACAATCTTGTAATTAAAATAATTGCAGTAAAATCTCTAGATAACATAGATGT

mRNA sequence

AACAAACTCTCCAAGAGATCAAAAAAGAATAAAAGGTATCTTAAAAAAAAAAAGAAAGAAGAAGAACAAGAATATAAGGTAGAAGTAGGAACAAGATGAGTATGAAGTTGAACTTGATGACTCTAACCTTCATACTATTATACGTTTCTATTAGGAAAGCCCAATCTAGTGATACTATTTTCGATGTGAGAAAGTACGGTGCTATACCTAATGCTGATATAACTCAGGCTCTAAAAAATGCATGGAAGGATGCCTGTGCATCAACGAGTCAAAGTACAATAATTATTCCAAACGTGGTTTACAAGTTAAGTGCAATTGAGTTAGTAGGACCATGTAAAGGTCCGATTGGAATTCAACTACAAGGGACATTGCAAGCTCCAGCAGATCTAACAGGTGAAGGTTGGGTTCATTTCAAATATATCGATCATTTTTCATTAACAGGTGGTGGAGTTTTTGACGGGCAAGGAAAACAAGCTTGGGAAAACAATGATTGTGATAAAAACCCTAGTTGTGCAAGAATTCCCATGAGTTTAAAGTTCAGCTTTATCACACATTCAATCGTGAGTGACATAACCTCTAAAGATAGCAAGAACTTTCATGTCCATGTATTGGGTTGTAATAACCTTACCCTACAACATGTAAATATAAATGCACCAGGAAATAGTATTAACACTGATGGGATTCACATTGGACGTTCTATTGGAATTAATATTATTAATACAAACATTGGTACTGGAGACGATTGTATTTCACTTGGAGACGGAAGCAAAGAAGTAACAATTACCAATGTGACATGTGGGCCTGGACATGGTATCAGTGTAGGAAGTTTAGGAAGGTATGTTGTTGAAGAACCAGTTGAGGGACTTCGAGTTAAGAATTGTACGTTAATGAATACATCAAATGGCTTGAGAATTAAAACATGGCCAGCTTCTCCCGCTGCTGGAACAGCTTCTGATATGCATTATGAAGATATAATTATGGTCAATGTCAGCAATCCCATTCTCGTAGATCAAGAATATTGTCCATACAACAAATGTAACAAAGAGATTCCATCTAAAGTCAAGATTAGTAAGGTTAGTTTCAAGAATATCAGAGGTACTTCTGGTAATGCACTCGCAGTAAAACTTGTTTGTAGTTCTAGTGTTCCATGTGAAGATGTTGAGCTAGCTGACATTGACCTCACTTACAATGGAAGTGAAGGCCCTATTTCATCTCAATGCTCAAATGTGAAGCCCACTGTTACTGGAAAGCAAAACCCACCTGCTTGTTCTTCCCTTGCTCTCGATTCTTCACCACCAGCTTAAACTATTCCTGCAAAGATGACCATTGGATACAAAAATTTTGAATGTTATTTTTTTTTACATAATTAGTGTTTAGTTTATATGATATAAGAGAATTTTGTAATTTCTCATTGTTTTGCCACTCAAGTTCTTATAGAAAAAACTTGGAGTGAATGTAGGCTTTTCCTCTTTTGAATTATCAAATGAAGATACATTTCTTCCTTAAATATTATTTTGGTTTATTAATTATACCCAACAATCTTGTAATTAAAATAATTGCAGTAAAATCTCTAGATAACATAGATGT

Coding sequence (CDS)

ATGAGTATGAAGTTGAACTTGATGACTCTAACCTTCATACTATTATACGTTTCTATTAGGAAAGCCCAATCTAGTGATACTATTTTCGATGTGAGAAAGTACGGTGCTATACCTAATGCTGATATAACTCAGGCTCTAAAAAATGCATGGAAGGATGCCTGTGCATCAACGAGTCAAAGTACAATAATTATTCCAAACGTGGTTTACAAGTTAAGTGCAATTGAGTTAGTAGGACCATGTAAAGGTCCGATTGGAATTCAACTACAAGGGACATTGCAAGCTCCAGCAGATCTAACAGGTGAAGGTTGGGTTCATTTCAAATATATCGATCATTTTTCATTAACAGGTGGTGGAGTTTTTGACGGGCAAGGAAAACAAGCTTGGGAAAACAATGATTGTGATAAAAACCCTAGTTGTGCAAGAATTCCCATGAGTTTAAAGTTCAGCTTTATCACACATTCAATCGTGAGTGACATAACCTCTAAAGATAGCAAGAACTTTCATGTCCATGTATTGGGTTGTAATAACCTTACCCTACAACATGTAAATATAAATGCACCAGGAAATAGTATTAACACTGATGGGATTCACATTGGACGTTCTATTGGAATTAATATTATTAATACAAACATTGGTACTGGAGACGATTGTATTTCACTTGGAGACGGAAGCAAAGAAGTAACAATTACCAATGTGACATGTGGGCCTGGACATGGTATCAGTGTAGGAAGTTTAGGAAGGTATGTTGTTGAAGAACCAGTTGAGGGACTTCGAGTTAAGAATTGTACGTTAATGAATACATCAAATGGCTTGAGAATTAAAACATGGCCAGCTTCTCCCGCTGCTGGAACAGCTTCTGATATGCATTATGAAGATATAATTATGGTCAATGTCAGCAATCCCATTCTCGTAGATCAAGAATATTGTCCATACAACAAATGTAACAAAGAGATTCCATCTAAAGTCAAGATTAGTAAGGTTAGTTTCAAGAATATCAGAGGTACTTCTGGTAATGCACTCGCAGTAAAACTTGTTTGTAGTTCTAGTGTTCCATGTGAAGATGTTGAGCTAGCTGACATTGACCTCACTTACAATGGAAGTGAAGGCCCTATTTCATCTCAATGCTCAAATGTGAAGCCCACTGTTACTGGAAAGCAAAACCCACCTGCTTGTTCTTCCCTTGCTCTCGATTCTTCACCACCAGCTTAA

Protein sequence

MSMKLNLMTLTFILLYVSIRKAQSSDTIFDVRKYGAIPNADITQALKNAWKDACASTSQSTIIIPNVVYKLSAIELVGPCKGPIGIQLQGTLQAPADLTGEGWVHFKYIDHFSLTGGGVFDGQGKQAWENNDCDKNPSCARIPMSLKFSFITHSIVSDITSKDSKNFHVHVLGCNNLTLQHVNINAPGNSINTDGIHIGRSIGINIINTNIGTGDDCISLGDGSKEVTITNVTCGPGHGISVGSLGRYVVEEPVEGLRVKNCTLMNTSNGLRIKTWPASPAAGTASDMHYEDIIMVNVSNPILVDQEYCPYNKCNKEIPSKVKISKVSFKNIRGTSGNALAVKLVCSSSVPCEDVELADIDLTYNGSEGPISSQCSNVKPTVTGKQNPPACSSLALDSSPPA
Homology
BLAST of Lsi08G003330 vs. ExPASy Swiss-Prot
Match: P24548 (Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1)

HSP 1 Score: 451.8 bits (1161), Expect = 8.0e-126
Identity = 221/359 (61.56%), Postives = 266/359 (74.09%), Query Frame = 0

Query: 41  DITQALKNAWKDACASTSQSTIIIPNVVYKLSAIELVGPCKGPIGIQLQGTLQAPAD--- 100
           D TQAL  AWK+ACAS S STI++P   + +  I L GPCK  IG+QLQGTL+APAD   
Sbjct: 1   DSTQALTTAWKEACASASPSTILVPKGNFAVGLITLEGPCKSSIGLQLQGTLKAPADPSK 60

Query: 101 LTGEGWVHFKYIDHFSLTGGGVFDGQGKQAWENNDCDKN-PSCARIPMSLKFSFITHSIV 160
           + G GW++   ID  ++ GGGVFDGQGK AW  NDC KN P C  + M+L+   +T+SI+
Sbjct: 61  IKGLGWINLNKIDLLTIFGGGVFDGQGKSAWVQNDCHKNGPICKTLSMNLRLYAVTNSIL 120

Query: 161 SDITSKDSKNFHVHVLGCNNLTLQHVNINAPGNSINTDGIHIGRSIGINIINTNIGTGDD 220
            D+T+ DSKNFHV+V+GC NLT +   I+A   SINTDGIHIGRS G+NIINT I TGDD
Sbjct: 121 RDVTTLDSKNFHVNVIGCKNLTFERFKISAAETSINTDGIHIGRSDGVNIINTEIKTGDD 180

Query: 221 CISLGDGSKEVTITNVTCGPGHGISVGSLGRYVVEEPVEGLRVKNCTLMNTSNGLRIKTW 280
           CISLGDGSK + ITN+TCGPGHGISVGSLGRY  EE V G+ VKNCT+  + NG+RIKTW
Sbjct: 181 CISLGDGSKNINITNITCGPGHGISVGSLGRYKNEESVVGIYVKNCTITGSQNGVRIKTW 240

Query: 281 PASPAAGTASDMHYEDIIMVNVSNPILVDQEYCPYNKCNKEIPSKVKISKVSFKNIRGTS 340
           P S   G AS+MH++DI M +V  PIL+DQ YCPYN+C  E+PS VK+SK+SFKNI+GTS
Sbjct: 241 PKS-EPGEASEMHFQDITMNSVGTPILIDQGYCPYNQCTAEVPSSVKLSKISFKNIKGTS 300

Query: 341 GNALAVKLVCSSSVPCEDVELADIDLTYNGSEGPISSQCSNVKPTVTGKQNPPACSSLA 396
               AVKLVCS S PC  VELADIDLTY+G  GP +S C N+KPT+ GKQ P  CS  A
Sbjct: 301 TTKEAVKLVCSKSFPCNGVELADIDLTYSGKGGPATSVCENIKPTIKGKQIPAICSGSA 358

BLAST of Lsi08G003330 vs. ExPASy Swiss-Prot
Match: Q05967 (Polygalacturonase OS=Nicotiana tabacum OX=4097 GN=PG1 PE=2 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 5.0e-120
Identity = 207/390 (53.08%), Postives = 285/390 (73.08%), Query Frame = 0

Query: 13  ILLYVSIRKAQSSDTIFDVRKYGAIPNADITQALKNAWKDACASTSQSTIIIPNVVYKLS 72
           +LL+++    +S   +FD+ KYGA  NADI++AL NA+K+AC STS STI+IP   + ++
Sbjct: 12  VLLFLA-HFGESQTGVFDITKYGANSNADISEALLNAFKEACQSTSPSTIVIPKGTFTMN 71

Query: 73  AIELVGPCKGPIGIQLQGTLQAPAD---LTGEGWVHFKYIDHFSLTGGGVFDGQGKQAWE 132
            ++L GPCK P+ +Q+Q TL+AP+D   L    W+    +D F+++GGG+ DGQ   AWE
Sbjct: 72  QVKLEGPCKSPLELQIQATLKAPSDPSQLKVGEWLTVNKLDQFTMSGGGILDGQAAAAWE 131

Query: 133 NNDCDKNPSCARIPMSLKFSFITHSIVSDITSKDSKNFHVHVLGCNNLTLQHVNINAPGN 192
              C ++  C ++P +L F+ +T+S + DIT+ DSK+FHV+V  C NLT    N++AP N
Sbjct: 132 ---CKQSKKCNKLPNNLSFNSLTNSTIKDITTLDSKSFHVNVNQCKNLTFIRFNVSAPAN 191

Query: 193 SINTDGIHIGRSIGINIINTNIGTGDDCISLGDGSKEVTITNVTCGPGHGISVGSLGRYV 252
           S NTDGIH+ RS  +NI ++N  TGDDCIS+GD ++++ IT VTCGPGHGISVGSLG   
Sbjct: 192 SPNTDGIHVSRSSSVNITDSNFSTGDDCISVGDETEQLYITRVTCGPGHGISVGSLGGNP 251

Query: 253 VEEPVEGLRVKNCTLMNTSNGLRIKTWPASPAAGTASDMHYEDIIMVNVSNPILVDQEYC 312
            E+PV G+ V+NCT  NT NG+RIKTWPAS   G  +D+H+EDII+ NVSNP+++DQ YC
Sbjct: 252 DEKPVVGVFVRNCTFTNTDNGVRIKTWPAS-HPGVVNDVHFEDIIVQNVSNPVVIDQVYC 311

Query: 313 PYNKCNKEIPSKVKISKVSFKNIRGTSGNALAVKLVCSSSVPCEDVELADIDLTYNGSEG 372
           P+NKCNK++PS+VKISKVSF+NI+GTS    AV L+ S  VPCE +E+ DID+TY+G EG
Sbjct: 312 PFNKCNKDLPSQVKISKVSFQNIKGTSRTQDAVSLLRSKGVPCEGIEVGDIDITYSGKEG 371

Query: 373 PISSQCSNVKPTVTGKQNPPACSSLALDSS 400
           P  S C N+KP++ GKQNPP C++ A  SS
Sbjct: 372 PAKSSCENIKPSLKGKQNPPVCTASAASSS 396

BLAST of Lsi08G003330 vs. ExPASy Swiss-Prot
Match: Q39786 (Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1)

HSP 1 Score: 432.2 bits (1110), Expect = 6.5e-120
Identity = 208/398 (52.26%), Postives = 286/398 (71.86%), Query Frame = 0

Query: 1   MSMKLNLMTLTFILLYVSIRKAQSSDTIFD-VRKYGAIPN--ADITQALKNAWKDACAST 60
           M+  LN++   F+LL + I  ++     FD V K+GA  +   D+++   +AWK+ACAS 
Sbjct: 1   MAPHLNIVPSMFVLLLLFISASKVQSDAFDVVAKFGAKADGKTDLSKPFLDAWKEACASV 60

Query: 61  SQSTIIIPNVVYKLSAIELVGPCKGPIGIQLQGTLQAPAD---LTGEGWVHFKYIDHFSL 120
           + ST++IP   Y LS + L GPCK PI I +QGT+QAPAD        WV F  +++F +
Sbjct: 61  TPSTVVIPKGTYLLSKVNLEGPCKAPIEINVQGTIQAPADPSAFKDPNWVRFYSVENFKM 120

Query: 121 TGGGVFDGQGKQAWENNDCDKNPSCARIPMSLKFSFITHSIVSDITSKDSKNFHVHVLGC 180
            GGG+FDGQG  A+E N C+     +++P++++F F+T++++ DITSKDSK FH++V  C
Sbjct: 121 FGGGIFDGQGSIAYEKNTCENREFRSKLPVNIRFDFLTNALIQDITSKDSKLFHINVFAC 180

Query: 181 NNLTLQHVNINAPGNSINTDGIHIGRSIGINIINTNIGTGDDCISLGDGSKEVTITNVTC 240
            N+TL+ + I AP  S NTDGIH+G+S G+NII ++I TGDDCIS+GDG+K + I  +TC
Sbjct: 181 KNITLERLKIEAPDESPNTDGIHMGKSEGVNIIASDIKTGDDCISIGDGTKNMVIKEITC 240

Query: 241 GPGHGISVGSLGRYVVEEPVEGLRVKNCTLMNTSNGLRIKTWPASPAAGTASDMHYEDII 300
           GPGHGIS+GSLG++  EEPVEG+++ NCT+ NTSNG RIKTWP     G  S++H+EDI 
Sbjct: 241 GPGHGISIGSLGKFQNEEPVEGIKISNCTITNTSNGARIKTWPGE-HGGAVSEIHFEDIT 300

Query: 301 MVNVSNPILVDQEYCPYNKCNKEIPSKVKISKVSFKNIRGTSGNALAVKLVCSSSVPCED 360
           M NVS+PIL+DQ+YCP+NKC K   SKVK+S +SFKNIRGTS    A+K +CS S PC++
Sbjct: 301 MNNVSSPILIDQQYCPWNKCKKNEESKVKLSNISFKNIRGTSALPEAIKFICSGSSPCQN 360

Query: 361 VELADIDLTYNGSEGPISSQCSNVKPTVTGKQNPPACS 393
           VELADID+ +NG+E P +SQC NVKP  +GK NP  CS
Sbjct: 361 VELADIDIKHNGAE-PATSQCLNVKPITSGKLNPIPCS 396

BLAST of Lsi08G003330 vs. ExPASy Swiss-Prot
Match: Q39766 (Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1)

HSP 1 Score: 431.4 bits (1108), Expect = 1.1e-119
Identity = 208/398 (52.26%), Postives = 285/398 (71.61%), Query Frame = 0

Query: 1   MSMKLNLMTLTFILLYVSIRKAQSSDTIFD-VRKYGAIPN--ADITQALKNAWKDACAST 60
           M+  LN++   F+LL + I  ++     FD V K+GA  +   D+++   +AWK+ACAS 
Sbjct: 1   MAPHLNIVPSMFVLLLLFISASKVQPDAFDVVAKFGAKADGKTDLSKPFLDAWKEACASV 60

Query: 61  SQSTIIIPNVVYKLSAIELVGPCKGPIGIQLQGTLQAPAD---LTGEGWVHFKYIDHFSL 120
           + ST++IP   Y LS + L GPCK PI I +QGT+QAPAD        WV F  +++F +
Sbjct: 61  TPSTVVIPKGTYLLSKVNLEGPCKAPIEINVQGTIQAPADPSAFKDPNWVRFYSVENFKM 120

Query: 121 TGGGVFDGQGKQAWENNDCDKNPSCARIPMSLKFSFITHSIVSDITSKDSKNFHVHVLGC 180
            GGG+FDGQG  A+E N C+     +++P++++F F+T++++ DITSKDSK FH++V  C
Sbjct: 121 FGGGIFDGQGSIAYEKNTCENREFRSKLPVNIRFDFVTNALIQDITSKDSKLFHINVFAC 180

Query: 181 NNLTLQHVNINAPGNSINTDGIHIGRSIGINIINTNIGTGDDCISLGDGSKEVTITNVTC 240
            N+TL+ + I AP  S NTDGIH+G+S G+NII ++I TGDDCIS+GDG+K + I  +TC
Sbjct: 181 KNITLERLKIEAPDESPNTDGIHMGKSEGVNIIASDIKTGDDCISIGDGTKNMVIKEITC 240

Query: 241 GPGHGISVGSLGRYVVEEPVEGLRVKNCTLMNTSNGLRIKTWPASPAAGTASDMHYEDII 300
           GPGHGIS+GSLG++  EEPVEG+++ NCT+ NTSNG RIKTWP     G  S++H+EDI 
Sbjct: 241 GPGHGISIGSLGKFQNEEPVEGIKISNCTITNTSNGARIKTWPGE-HGGAVSEIHFEDIT 300

Query: 301 MVNVSNPILVDQEYCPYNKCNKEIPSKVKISKVSFKNIRGTSGNALAVKLVCSSSVPCED 360
           M NVS+PIL+DQ+YCP+NKC K   SKVK+S +SFKNIRGTS    A+K +CS S PC++
Sbjct: 301 MNNVSSPILIDQQYCPWNKCKKNEESKVKLSNISFKNIRGTSALPEAIKFICSGSSPCQN 360

Query: 361 VELADIDLTYNGSEGPISSQCSNVKPTVTGKQNPPACS 393
           VELADID+ +NG+E P +SQC NVKP   GK NP  CS
Sbjct: 361 VELADIDIQHNGAE-PATSQCLNVKPITIGKLNPIPCS 396

BLAST of Lsi08G003330 vs. ExPASy Swiss-Prot
Match: Q40312 (Polygalacturonase OS=Medicago sativa OX=3879 PE=2 SV=1)

HSP 1 Score: 427.9 bits (1099), Expect = 1.2e-118
Identity = 210/399 (52.63%), Postives = 283/399 (70.93%), Query Frame = 0

Query: 3   MKLNLMTLTFILLYVSIRKAQSSDTIFDVRKYGAIPNADITQALKNAWKDACASTSQSTI 62
           MK +   +   L       AQS   + D+ K+G  PN+DI QAL +AW +ACAST+ + I
Sbjct: 1   MKFSTAIIVSFLFIADFCAAQSG--VLDISKFGGKPNSDIGQALTSAWNEACASTTAAKI 60

Query: 63  IIPNVVYKLSAIELVGPCKGPIGIQLQGTLQAPADLT----GEGWVHFKYIDHFSLTGGG 122
           +IP   Y+L+ IEL GPCK PI +Q+ GT+QAPAD +     E W  F Y+DH +L+G G
Sbjct: 61  VIPAGTYQLNGIELKGPCKAPIELQVDGTIQAPADPSVIKGTEQWFKFLYMDHLTLSGKG 120

Query: 123 VFDGQG----KQAWENNDCDKNPSCARIPMSLKFSFITHSIVSDITSKDSKNFHVHVLGC 182
           VFDGQG    K+A   +      S +++ M+  F+F+ +SIV  +TSKDSKNFHV V GC
Sbjct: 121 VFDGQGATVYKKAAPASAWSGKNSNSKVFMNFGFNFVNNSIVRGVTSKDSKNFHVMVFGC 180

Query: 183 NNLTLQHVNINAPGNSINTDGIHIGRSIGINIINTNIGTGDDCISLGDGSKEVTITNVTC 242
            N+T     I APG+S NTDGIH+G+S  + I+NTNIGTGDDC+S+GDGSK++T+  V C
Sbjct: 181 KNITFDGFTITAPGDSPNTDGIHMGKSTDVKILNTNIGTGDDCVSIGDGSKQITVQGVNC 240

Query: 243 GPGHGISVGSLGRYVVEEPVEGLRVKNCTLMNTSNGLRIKTWPASPAAGTASDMHYEDII 302
           GPGHG+SVGSLG++  EE VEG+ VKNCTL  T NG+RIKTWP +P   T SD+H+EDI 
Sbjct: 241 GPGHGLSVGSLGKFTTEENVEGITVKNCTLTATDNGVRIKTWPDAPGTITVSDIHFEDIT 300

Query: 303 MVNVSNPILVDQEYCPYNKCNKEIPSKVKISKVSFKNIRGTSGNALAVKLVCSSSVPCED 362
           M NV NP+++DQEY P+N+C+K+ PSK+K+SK+SFKN++GTSG A  V L+CSS+VPC+ 
Sbjct: 301 MTNVKNPVIIDQEYYPWNQCSKKNPSKIKLSKISFKNVKGTSGTAEGVVLICSSAVPCDG 360

Query: 363 VELADIDLTYNGSEGPISSQCSNVKPTVTGKQNPPACSS 394
           VEL ++DL +NG+  P +++C+NVKP VTG    P C +
Sbjct: 361 VELNNVDLKFNGA--PTTAKCTNVKPLVTG--TAPVCQA 393

BLAST of Lsi08G003330 vs. ExPASy TrEMBL
Match: A0A6J1DUC6 (exopolygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111024096 PE=3 SV=1)

HSP 1 Score: 665.6 bits (1716), Expect = 1.3e-187
Identity = 317/402 (78.86%), Postives = 358/402 (89.05%), Query Frame = 0

Query: 1   MSMKLNLMTLTFILLYVSIRKAQSSDTIFDVRKYGAIPNADITQALKNAWKDACASTSQS 60
           M M+LNL+ L+ +LL+VS  KAQSSD IFDV K+GA+PNADI++AL +AWK+ACAS  QS
Sbjct: 1   MGMELNLLILSVVLLFVSTTKAQSSDGIFDVTKFGAMPNADISKALMSAWKEACASPIQS 60

Query: 61  TIIIPNVVYKLSAIELVGPCKGPIGIQLQGTLQAPADLTGEGWVHFKYIDHFSLTGGGVF 120
            +IIP V YKL  I+L GPCK PI +QLQGT+QAP DLTGEGWV FKYIDHF+L+GGG F
Sbjct: 61  KVIIPKVAYKLGTIDLTGPCKSPIEVQLQGTIQAPIDLTGEGWVQFKYIDHFTLSGGGTF 120

Query: 121 DGQGKQAWENNDCDKNPSCARIPMSLKFSFITHSIVSDITSKDSKNFHVHVLGCNNLTLQ 180
           DGQGK AWE NDCDKNP C R+PMSL+FSFIT+SIVSDITSKDSKNFHV +LGCNNLT Q
Sbjct: 121 DGQGKAAWEKNDCDKNPKCDRLPMSLRFSFITNSIVSDITSKDSKNFHVQILGCNNLTFQ 180

Query: 181 HVNINAPGNSINTDGIHIGRSIGINIINTNIGTGDDCISLGDGSKEVTITNVTCGPGHGI 240
           HVN+NAPG+SINTDGIHIGRS GINI++TNIGTGDDCISLGDGSK+VTITNVTCGPGHGI
Sbjct: 181 HVNVNAPGDSINTDGIHIGRSTGINILSTNIGTGDDCISLGDGSKQVTITNVTCGPGHGI 240

Query: 241 SVGSLGRYVVEEPVEGLRVKNCTLMNTSNGLRIKTWPASPAAGTASDMHYEDIIMVNVSN 300
           SVGSLGRY  EEPVEG+R  NCT+MNTSNG+RIKTWPASP AGTASDMH+ED+IMVNVSN
Sbjct: 241 SVGSLGRYTAEEPVEGIRATNCTIMNTSNGVRIKTWPASPTAGTASDMHFEDMIMVNVSN 300

Query: 301 PILVDQEYCPYNKCNKEIPSKVKISKVSFKNIRGTSGNALAVKLVCSSSVPCEDVELADI 360
           PIL+DQEYCPYN+CNK+ PSKVKISKVSFKN+RG+S NALAVKLVCS+ VPCEDVE+ADI
Sbjct: 301 PILIDQEYCPYNQCNKKSPSKVKISKVSFKNVRGSSANALAVKLVCSAEVPCEDVEVADI 360

Query: 361 DLTYNGSEGPISSQCSNVKPTVTGKQNPPACSSLALDSSPPA 403
           DLTYNG+EGPISSQC NVKP ++GKQNP ACSS A    PPA
Sbjct: 361 DLTYNGNEGPISSQCKNVKPIISGKQNPRACSSPA----PPA 398

BLAST of Lsi08G003330 vs. ExPASy TrEMBL
Match: A0A6J1DBN7 (exopolygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111019009 PE=3 SV=1)

HSP 1 Score: 652.1 bits (1681), Expect = 1.5e-183
Identity = 310/401 (77.31%), Postives = 356/401 (88.78%), Query Frame = 0

Query: 1   MSMKLNLMTLTFILLYVSIRKAQSSDTIFDVRKYGAIPNADITQALKNAWKDACASTSQS 60
           MS++LNL+ L+F+LL+VS  KAQS+D +FDV KYGA P  DI+QAL  AWK+ACAST+QS
Sbjct: 5   MSIELNLVALSFVLLFVSRGKAQSTDGVFDVTKYGAKPGTDISQALLKAWKEACASTTQS 64

Query: 61  TIIIPNVVYKLSAIELVGPCKGPIGIQLQGTLQAPADLTGEGWVHFKYIDHFSLTGGGVF 120
            +IIP   Y+L+ ++LVGPCK PIGI+ QGTLQAP D TGEGWV+FKYIDHFSLTGGGVF
Sbjct: 65  KVIIPKASYQLTGVDLVGPCKSPIGIEFQGTLQAPIDPTGEGWVNFKYIDHFSLTGGGVF 124

Query: 121 DGQGKQAWENNDCDKNPSCARIPMSLKFSFITHSIVSDITSKDSKNFHVHVLGCNNLTLQ 180
           DGQGK AWE NDC +NP+CA+ PMSLKFSFIT+SIVS+ITSKDSKNFHV VLGCNNLT +
Sbjct: 125 DGQGKLAWEKNDCGQNPNCAQFPMSLKFSFITNSIVSEITSKDSKNFHVFVLGCNNLTFE 184

Query: 181 HVNINAPGNSINTDGIHIGRSIGINIINTNIGTGDDCISLGDGSKEVTITNVTCGPGHGI 240
           HV I+APG+SINTDGIHIGRS  +N+++T IGTGDDCISLGDGSK VTI NVTCGPGHGI
Sbjct: 185 HVTIDAPGHSINTDGIHIGRSNEVNVLSTRIGTGDDCISLGDGSKHVTINNVTCGPGHGI 244

Query: 241 SVGSLGRYVVEEPVEGLRVKNCTLMNTSNGLRIKTWPASPAAGTASDMHYEDIIMVNVSN 300
           SVGSLGRY VEEPVEG+ VKNCT  NT NG+RIKTWPASP  GTA++MHYEDIIMVNVSN
Sbjct: 245 SVGSLGRYTVEEPVEGIIVKNCTFTNTDNGVRIKTWPASPTPGTATNMHYEDIIMVNVSN 304

Query: 301 PILVDQEYCPYNKCNKEIPSKVKISKVSFKNIRGTSGNALAVKLVCSSSVPCEDVELADI 360
           PIL+DQEYCPYN+CNK+IPSKVKISKVSFKNIRG+S NALAVK++CSS  PCE VE+ADI
Sbjct: 305 PILIDQEYCPYNQCNKKIPSKVKISKVSFKNIRGSSANALAVKIICSSDFPCEGVEIADI 364

Query: 361 DLTYNGSEGPISSQCSNVKPTVTGKQNPPACSSLALDSSPP 402
           DLTYNGSEGPI+SQCSNVKP ++GKQNPPACSS+A  +SPP
Sbjct: 365 DLTYNGSEGPITSQCSNVKPIISGKQNPPACSSVAPIASPP 405

BLAST of Lsi08G003330 vs. ExPASy TrEMBL
Match: A0A5A7T7B3 (Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001590 PE=3 SV=1)

HSP 1 Score: 635.6 bits (1638), Expect = 1.4e-178
Identity = 319/446 (71.52%), Postives = 354/446 (79.37%), Query Frame = 0

Query: 1   MSMKLNLM-TLTFILLYVSIRKAQS----------------------------------- 60
           MSMKLN++ TL FILLYVS  ++QS                                   
Sbjct: 1   MSMKLNMVTTLAFILLYVSFVRSQSGGAATPAGAASAGAAPAGATPAGATPAGAAAAGAT 60

Query: 61  -----------SDTIFDVRKYGAIPNADITQALKNAWKDACASTSQSTIIIPNVVYKLSA 120
                         IFDV KYGA PNADITQAL +AWK+ACASTS   I+IP   YKL  
Sbjct: 61  PAGAAAAGATPGGAIFDVTKYGAKPNADITQALADAWKEACASTSPCKIVIPTGTYKLGT 120

Query: 121 IELVGPCKGPIGIQLQGTLQAPADLTGEGWVHFKYIDHFSLTGGGVFDGQGKQAWENNDC 180
           IEL G CK PI IQ+QGTLQAP DLTGE WVHFKYID+ S+T GGVFDGQGKQAWE+NDC
Sbjct: 121 IELKGECKSPIEIQVQGTLQAPPDLTGEDWVHFKYIDYLSVTSGGVFDGQGKQAWESNDC 180

Query: 181 DKNPSCARIPMSLKFSFITHSIVSDITSKDSKNFHVHVLGCNNLTLQHVNINAPGNSINT 240
           DKNP CARIPM+LKFSFI ++IVSDITSKDSKNFH+ VLGCNNLT QHVN+NAP  SINT
Sbjct: 181 DKNPKCARIPMNLKFSFIKNAIVSDITSKDSKNFHIQVLGCNNLTFQHVNVNAPETSINT 240

Query: 241 DGIHIGRSIGINIINTNIGTGDDCISLGDGSKEVTITNVTCGPGHGISVGSLGRYVVEEP 300
           DGIHIGRS+GINI+NTNIGTGDDCISLGDGSK+VTITNVTCGPGHGISVGSLGRY +EEP
Sbjct: 241 DGIHIGRSLGINILNTNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSLGRYNLEEP 300

Query: 301 VEGLRVKNCTLMNTSNGLRIKTWPASPAAGTASDMHYEDIIMVNVSNPILVDQEYCPYNK 360
           VEGL+VKNCTL  T+NGLRIKTWPASPAAG ASDMHYED++M NV NPIL+DQEYCPYN+
Sbjct: 301 VEGLQVKNCTLTGTTNGLRIKTWPASPAAGVASDMHYEDVVMNNVMNPILIDQEYCPYNQ 360

Query: 361 CNKEIPSKVKISKVSFKNIRGTSGNALAVKLVCSSSVPCEDVELADIDLTYNGSEGPISS 400
           CNK++PSKVKISKVSFKNIRGTSGNA AV LVCSSS PC+ VELADIDLTY+GSEGPI+S
Sbjct: 361 CNKQVPSKVKISKVSFKNIRGTSGNAAAVTLVCSSSNPCDGVELADIDLTYSGSEGPITS 420

BLAST of Lsi08G003330 vs. ExPASy TrEMBL
Match: A0A1S3CC08 (exopolygalacturonase-like OS=Cucumis melo OX=3656 GN=LOC103498752 PE=3 SV=1)

HSP 1 Score: 634.0 bits (1634), Expect = 4.2e-178
Identity = 307/394 (77.92%), Postives = 343/394 (87.06%), Query Frame = 0

Query: 8   MTLTFILLYVSIRKAQSS--DTIFDVRKYGAIPNADITQALKNAWKDACASTSQSTIIIP 67
           MT+   L+ +S   A ++    IFDV KYGA PNADITQAL +AWK+ACASTS   I+IP
Sbjct: 1   MTVPIFLVVLSSAAAGATPGGAIFDVTKYGAKPNADITQALADAWKEACASTSPCKIVIP 60

Query: 68  NVVYKLSAIELVGPCKGPIGIQLQGTLQAPADLTGEGWVHFKYIDHFSLTGGGVFDGQGK 127
              YKL  IEL G CK PI IQ+QGTLQAP DLTGE WVHFKYID+ S+T GGVFDGQGK
Sbjct: 61  TGTYKLGTIELKGECKSPIEIQVQGTLQAPPDLTGEDWVHFKYIDYLSVTSGGVFDGQGK 120

Query: 128 QAWENNDCDKNPSCARIPMSLKFSFITHSIVSDITSKDSKNFHVHVLGCNNLTLQHVNIN 187
           QAWE+NDCDKNP CARIPM+LKFSFI ++IVSDITSKDSKNFH+ VLGCNNLT QHVN+N
Sbjct: 121 QAWESNDCDKNPKCARIPMNLKFSFIKNAIVSDITSKDSKNFHIQVLGCNNLTFQHVNVN 180

Query: 188 APGNSINTDGIHIGRSIGINIINTNIGTGDDCISLGDGSKEVTITNVTCGPGHGISVGSL 247
           AP  SINTDGIHIGRS+GINI+NTNIGTGDDCISLGDGSK+VTITNVTCGPGHGISVGSL
Sbjct: 181 APETSINTDGIHIGRSLGINILNTNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSL 240

Query: 248 GRYVVEEPVEGLRVKNCTLMNTSNGLRIKTWPASPAAGTASDMHYEDIIMVNVSNPILVD 307
           GRY +EEPVEGL+VKNCTL  T+NGLRIKTWPASPAAG ASDMHYED++M NV NPIL+D
Sbjct: 241 GRYNLEEPVEGLQVKNCTLTGTTNGLRIKTWPASPAAGVASDMHYEDVVMNNVMNPILID 300

Query: 308 QEYCPYNKCNKEIPSKVKISKVSFKNIRGTSGNALAVKLVCSSSVPCEDVELADIDLTYN 367
           QEYCPYN+CNK++PSKVKISKVSFKNIRGTSGNA AV LVCSSS PC+ VELADIDLTY+
Sbjct: 301 QEYCPYNQCNKQVPSKVKISKVSFKNIRGTSGNAAAVTLVCSSSNPCDGVELADIDLTYS 360

Query: 368 GSEGPISSQCSNVKPTVTGKQNPPACSSLALDSS 400
           GSEGPI+S+C+NVKPTV+GKQNPPAC+S A   S
Sbjct: 361 GSEGPITSRCANVKPTVSGKQNPPACTSPATPKS 394

BLAST of Lsi08G003330 vs. ExPASy TrEMBL
Match: A0A6J1HJP4 (exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111465122 PE=3 SV=1)

HSP 1 Score: 600.5 bits (1547), Expect = 5.1e-168
Identity = 284/394 (72.08%), Postives = 331/394 (84.01%), Query Frame = 0

Query: 1   MSMKLNLMTLTFI-LLYVSIRKAQSSDTIFDVRKYGAIPNADITQALKNAWKDACASTSQ 60
           M M +  +TL+F+ LLYVSIR AQSSD +FDV  YGA PNADITQ L  AWKDACAS  Q
Sbjct: 1   MGMNMRSITLSFVLLLYVSIRTAQSSDGVFDVTTYGAKPNADITQILDKAWKDACASPIQ 60

Query: 61  STIIIPNVVYKLSAIELVGPCKGPIGIQLQGTLQAPADLTGEGWVHFKYIDHFSLTGGGV 120
           S +IIP   Y L  I L+GPCK PI I +QGT+ AP D+TG+GW+ F+Y+D  SLTGGGV
Sbjct: 61  SKLIIPKETYMLGVITLLGPCKSPIDIVVQGTIMAPVDITGDGWIQFRYVDKISLTGGGV 120

Query: 121 FDGQGKQAWENNDCDKNPSCARIPMSLKFSFITHSIVSDITSKDSKNFHVHVLGCNNLTL 180
           FDGQG++AWE NDC KNP CARIPMSLKFSF+  SIV DITSKDSKNFHV++LGC NLT 
Sbjct: 121 FDGQGQKAWERNDCHKNPKCARIPMSLKFSFVKDSIVRDITSKDSKNFHVNILGCKNLTF 180

Query: 181 QHVNINAPGNSINTDGIHIGRSIGINIINTNIGTGDDCISLGDGSKEVTITNVTCGPGHG 240
            HV INAPG SIN+DGIH+GR  G+NIIN+ IGTGDDCISLGDGS+EVTITNVTCGPGHG
Sbjct: 181 DHVTINAPGTSINSDGIHMGRCDGVNIINSVIGTGDDCISLGDGSREVTITNVTCGPGHG 240

Query: 241 ISVGSLGRYVVEEPVEGLRVKNCTLMNTSNGLRIKTWPASPAAGTASDMHYEDIIMVNVS 300
           ISVGSLG+Y+VEEPV GL VKNCTL+ T+NGLRIKTWPASPA G+ +DMHYEDI++V+V 
Sbjct: 241 ISVGSLGQYLVEEPVAGLYVKNCTLIGTTNGLRIKTWPASPATGSVTDMHYEDIVLVDVQ 300

Query: 301 NPILVDQEYCPYNKCNKEIPSKVKISKVSFKNIRGTSGNALAVKLVCSSSVPCEDVELAD 360
           NPI++DQEYCPYNKCN++IPSK+KISKVSFKNIRG++GNA+AVKL+CS+  PCEDV L+D
Sbjct: 301 NPIILDQEYCPYNKCNRKIPSKIKISKVSFKNIRGSAGNAVAVKLLCSADYPCEDVTLSD 360

Query: 361 IDLTYNGSEGPISSQCSNVKPTVTGKQNPPACSS 394
           ID+TY G EGP+ S+CSNVKP VTG Q P  C S
Sbjct: 361 IDITYTGREGPVVSECSNVKPVVTGIQKPIICPS 394

BLAST of Lsi08G003330 vs. NCBI nr
Match: XP_022157387.1 (exopolygalacturonase-like [Momordica charantia] >XP_022159278.1 exopolygalacturonase-like [Momordica charantia])

HSP 1 Score: 665.6 bits (1716), Expect = 2.7e-187
Identity = 317/402 (78.86%), Postives = 358/402 (89.05%), Query Frame = 0

Query: 1   MSMKLNLMTLTFILLYVSIRKAQSSDTIFDVRKYGAIPNADITQALKNAWKDACASTSQS 60
           M M+LNL+ L+ +LL+VS  KAQSSD IFDV K+GA+PNADI++AL +AWK+ACAS  QS
Sbjct: 1   MGMELNLLILSVVLLFVSTTKAQSSDGIFDVTKFGAMPNADISKALMSAWKEACASPIQS 60

Query: 61  TIIIPNVVYKLSAIELVGPCKGPIGIQLQGTLQAPADLTGEGWVHFKYIDHFSLTGGGVF 120
            +IIP V YKL  I+L GPCK PI +QLQGT+QAP DLTGEGWV FKYIDHF+L+GGG F
Sbjct: 61  KVIIPKVAYKLGTIDLTGPCKSPIEVQLQGTIQAPIDLTGEGWVQFKYIDHFTLSGGGTF 120

Query: 121 DGQGKQAWENNDCDKNPSCARIPMSLKFSFITHSIVSDITSKDSKNFHVHVLGCNNLTLQ 180
           DGQGK AWE NDCDKNP C R+PMSL+FSFIT+SIVSDITSKDSKNFHV +LGCNNLT Q
Sbjct: 121 DGQGKAAWEKNDCDKNPKCDRLPMSLRFSFITNSIVSDITSKDSKNFHVQILGCNNLTFQ 180

Query: 181 HVNINAPGNSINTDGIHIGRSIGINIINTNIGTGDDCISLGDGSKEVTITNVTCGPGHGI 240
           HVN+NAPG+SINTDGIHIGRS GINI++TNIGTGDDCISLGDGSK+VTITNVTCGPGHGI
Sbjct: 181 HVNVNAPGDSINTDGIHIGRSTGINILSTNIGTGDDCISLGDGSKQVTITNVTCGPGHGI 240

Query: 241 SVGSLGRYVVEEPVEGLRVKNCTLMNTSNGLRIKTWPASPAAGTASDMHYEDIIMVNVSN 300
           SVGSLGRY  EEPVEG+R  NCT+MNTSNG+RIKTWPASP AGTASDMH+ED+IMVNVSN
Sbjct: 241 SVGSLGRYTAEEPVEGIRATNCTIMNTSNGVRIKTWPASPTAGTASDMHFEDMIMVNVSN 300

Query: 301 PILVDQEYCPYNKCNKEIPSKVKISKVSFKNIRGTSGNALAVKLVCSSSVPCEDVELADI 360
           PIL+DQEYCPYN+CNK+ PSKVKISKVSFKN+RG+S NALAVKLVCS+ VPCEDVE+ADI
Sbjct: 301 PILIDQEYCPYNQCNKKSPSKVKISKVSFKNVRGSSANALAVKLVCSAEVPCEDVEVADI 360

Query: 361 DLTYNGSEGPISSQCSNVKPTVTGKQNPPACSSLALDSSPPA 403
           DLTYNG+EGPISSQC NVKP ++GKQNP ACSS A    PPA
Sbjct: 361 DLTYNGNEGPISSQCKNVKPIISGKQNPRACSSPA----PPA 398

BLAST of Lsi08G003330 vs. NCBI nr
Match: XP_022150984.1 (exopolygalacturonase-like [Momordica charantia])

HSP 1 Score: 652.1 bits (1681), Expect = 3.1e-183
Identity = 310/401 (77.31%), Postives = 356/401 (88.78%), Query Frame = 0

Query: 1   MSMKLNLMTLTFILLYVSIRKAQSSDTIFDVRKYGAIPNADITQALKNAWKDACASTSQS 60
           MS++LNL+ L+F+LL+VS  KAQS+D +FDV KYGA P  DI+QAL  AWK+ACAST+QS
Sbjct: 5   MSIELNLVALSFVLLFVSRGKAQSTDGVFDVTKYGAKPGTDISQALLKAWKEACASTTQS 64

Query: 61  TIIIPNVVYKLSAIELVGPCKGPIGIQLQGTLQAPADLTGEGWVHFKYIDHFSLTGGGVF 120
            +IIP   Y+L+ ++LVGPCK PIGI+ QGTLQAP D TGEGWV+FKYIDHFSLTGGGVF
Sbjct: 65  KVIIPKASYQLTGVDLVGPCKSPIGIEFQGTLQAPIDPTGEGWVNFKYIDHFSLTGGGVF 124

Query: 121 DGQGKQAWENNDCDKNPSCARIPMSLKFSFITHSIVSDITSKDSKNFHVHVLGCNNLTLQ 180
           DGQGK AWE NDC +NP+CA+ PMSLKFSFIT+SIVS+ITSKDSKNFHV VLGCNNLT +
Sbjct: 125 DGQGKLAWEKNDCGQNPNCAQFPMSLKFSFITNSIVSEITSKDSKNFHVFVLGCNNLTFE 184

Query: 181 HVNINAPGNSINTDGIHIGRSIGINIINTNIGTGDDCISLGDGSKEVTITNVTCGPGHGI 240
           HV I+APG+SINTDGIHIGRS  +N+++T IGTGDDCISLGDGSK VTI NVTCGPGHGI
Sbjct: 185 HVTIDAPGHSINTDGIHIGRSNEVNVLSTRIGTGDDCISLGDGSKHVTINNVTCGPGHGI 244

Query: 241 SVGSLGRYVVEEPVEGLRVKNCTLMNTSNGLRIKTWPASPAAGTASDMHYEDIIMVNVSN 300
           SVGSLGRY VEEPVEG+ VKNCT  NT NG+RIKTWPASP  GTA++MHYEDIIMVNVSN
Sbjct: 245 SVGSLGRYTVEEPVEGIIVKNCTFTNTDNGVRIKTWPASPTPGTATNMHYEDIIMVNVSN 304

Query: 301 PILVDQEYCPYNKCNKEIPSKVKISKVSFKNIRGTSGNALAVKLVCSSSVPCEDVELADI 360
           PIL+DQEYCPYN+CNK+IPSKVKISKVSFKNIRG+S NALAVK++CSS  PCE VE+ADI
Sbjct: 305 PILIDQEYCPYNQCNKKIPSKVKISKVSFKNIRGSSANALAVKIICSSDFPCEGVEIADI 364

Query: 361 DLTYNGSEGPISSQCSNVKPTVTGKQNPPACSSLALDSSPP 402
           DLTYNGSEGPI+SQCSNVKP ++GKQNPPACSS+A  +SPP
Sbjct: 365 DLTYNGSEGPITSQCSNVKPIISGKQNPPACSSVAPIASPP 405

BLAST of Lsi08G003330 vs. NCBI nr
Match: KAA0039364.1 (exopolygalacturonase-like [Cucumis melo var. makuwa] >TYK00547.1 exopolygalacturonase-like [Cucumis melo var. makuwa])

HSP 1 Score: 635.6 bits (1638), Expect = 3.0e-178
Identity = 319/446 (71.52%), Postives = 354/446 (79.37%), Query Frame = 0

Query: 1   MSMKLNLM-TLTFILLYVSIRKAQS----------------------------------- 60
           MSMKLN++ TL FILLYVS  ++QS                                   
Sbjct: 1   MSMKLNMVTTLAFILLYVSFVRSQSGGAATPAGAASAGAAPAGATPAGATPAGAAAAGAT 60

Query: 61  -----------SDTIFDVRKYGAIPNADITQALKNAWKDACASTSQSTIIIPNVVYKLSA 120
                         IFDV KYGA PNADITQAL +AWK+ACASTS   I+IP   YKL  
Sbjct: 61  PAGAAAAGATPGGAIFDVTKYGAKPNADITQALADAWKEACASTSPCKIVIPTGTYKLGT 120

Query: 121 IELVGPCKGPIGIQLQGTLQAPADLTGEGWVHFKYIDHFSLTGGGVFDGQGKQAWENNDC 180
           IEL G CK PI IQ+QGTLQAP DLTGE WVHFKYID+ S+T GGVFDGQGKQAWE+NDC
Sbjct: 121 IELKGECKSPIEIQVQGTLQAPPDLTGEDWVHFKYIDYLSVTSGGVFDGQGKQAWESNDC 180

Query: 181 DKNPSCARIPMSLKFSFITHSIVSDITSKDSKNFHVHVLGCNNLTLQHVNINAPGNSINT 240
           DKNP CARIPM+LKFSFI ++IVSDITSKDSKNFH+ VLGCNNLT QHVN+NAP  SINT
Sbjct: 181 DKNPKCARIPMNLKFSFIKNAIVSDITSKDSKNFHIQVLGCNNLTFQHVNVNAPETSINT 240

Query: 241 DGIHIGRSIGINIINTNIGTGDDCISLGDGSKEVTITNVTCGPGHGISVGSLGRYVVEEP 300
           DGIHIGRS+GINI+NTNIGTGDDCISLGDGSK+VTITNVTCGPGHGISVGSLGRY +EEP
Sbjct: 241 DGIHIGRSLGINILNTNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSLGRYNLEEP 300

Query: 301 VEGLRVKNCTLMNTSNGLRIKTWPASPAAGTASDMHYEDIIMVNVSNPILVDQEYCPYNK 360
           VEGL+VKNCTL  T+NGLRIKTWPASPAAG ASDMHYED++M NV NPIL+DQEYCPYN+
Sbjct: 301 VEGLQVKNCTLTGTTNGLRIKTWPASPAAGVASDMHYEDVVMNNVMNPILIDQEYCPYNQ 360

Query: 361 CNKEIPSKVKISKVSFKNIRGTSGNALAVKLVCSSSVPCEDVELADIDLTYNGSEGPISS 400
           CNK++PSKVKISKVSFKNIRGTSGNA AV LVCSSS PC+ VELADIDLTY+GSEGPI+S
Sbjct: 361 CNKQVPSKVKISKVSFKNIRGTSGNAAAVTLVCSSSNPCDGVELADIDLTYSGSEGPITS 420

BLAST of Lsi08G003330 vs. NCBI nr
Match: XP_008459709.2 (PREDICTED: exopolygalacturonase-like [Cucumis melo])

HSP 1 Score: 634.0 bits (1634), Expect = 8.6e-178
Identity = 307/394 (77.92%), Postives = 343/394 (87.06%), Query Frame = 0

Query: 8   MTLTFILLYVSIRKAQSS--DTIFDVRKYGAIPNADITQALKNAWKDACASTSQSTIIIP 67
           MT+   L+ +S   A ++    IFDV KYGA PNADITQAL +AWK+ACASTS   I+IP
Sbjct: 1   MTVPIFLVVLSSAAAGATPGGAIFDVTKYGAKPNADITQALADAWKEACASTSPCKIVIP 60

Query: 68  NVVYKLSAIELVGPCKGPIGIQLQGTLQAPADLTGEGWVHFKYIDHFSLTGGGVFDGQGK 127
              YKL  IEL G CK PI IQ+QGTLQAP DLTGE WVHFKYID+ S+T GGVFDGQGK
Sbjct: 61  TGTYKLGTIELKGECKSPIEIQVQGTLQAPPDLTGEDWVHFKYIDYLSVTSGGVFDGQGK 120

Query: 128 QAWENNDCDKNPSCARIPMSLKFSFITHSIVSDITSKDSKNFHVHVLGCNNLTLQHVNIN 187
           QAWE+NDCDKNP CARIPM+LKFSFI ++IVSDITSKDSKNFH+ VLGCNNLT QHVN+N
Sbjct: 121 QAWESNDCDKNPKCARIPMNLKFSFIKNAIVSDITSKDSKNFHIQVLGCNNLTFQHVNVN 180

Query: 188 APGNSINTDGIHIGRSIGINIINTNIGTGDDCISLGDGSKEVTITNVTCGPGHGISVGSL 247
           AP  SINTDGIHIGRS+GINI+NTNIGTGDDCISLGDGSK+VTITNVTCGPGHGISVGSL
Sbjct: 181 APETSINTDGIHIGRSLGINILNTNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSL 240

Query: 248 GRYVVEEPVEGLRVKNCTLMNTSNGLRIKTWPASPAAGTASDMHYEDIIMVNVSNPILVD 307
           GRY +EEPVEGL+VKNCTL  T+NGLRIKTWPASPAAG ASDMHYED++M NV NPIL+D
Sbjct: 241 GRYNLEEPVEGLQVKNCTLTGTTNGLRIKTWPASPAAGVASDMHYEDVVMNNVMNPILID 300

Query: 308 QEYCPYNKCNKEIPSKVKISKVSFKNIRGTSGNALAVKLVCSSSVPCEDVELADIDLTYN 367
           QEYCPYN+CNK++PSKVKISKVSFKNIRGTSGNA AV LVCSSS PC+ VELADIDLTY+
Sbjct: 301 QEYCPYNQCNKQVPSKVKISKVSFKNIRGTSGNAAAVTLVCSSSNPCDGVELADIDLTYS 360

Query: 368 GSEGPISSQCSNVKPTVTGKQNPPACSSLALDSS 400
           GSEGPI+S+C+NVKPTV+GKQNPPAC+S A   S
Sbjct: 361 GSEGPITSRCANVKPTVSGKQNPPACTSPATPKS 394

BLAST of Lsi08G003330 vs. NCBI nr
Match: XP_022965192.1 (exopolygalacturonase-like [Cucurbita maxima])

HSP 1 Score: 600.5 bits (1547), Expect = 1.1e-167
Identity = 284/394 (72.08%), Postives = 331/394 (84.01%), Query Frame = 0

Query: 1   MSMKLNLMTLTFI-LLYVSIRKAQSSDTIFDVRKYGAIPNADITQALKNAWKDACASTSQ 60
           M M +  +TL+F+ LLYVSIR AQSSD +FDV  YGA PNADITQ L  AWKDACAS  Q
Sbjct: 1   MGMNMRSITLSFVLLLYVSIRTAQSSDGVFDVTTYGAKPNADITQILDKAWKDACASPIQ 60

Query: 61  STIIIPNVVYKLSAIELVGPCKGPIGIQLQGTLQAPADLTGEGWVHFKYIDHFSLTGGGV 120
           S +IIP   Y L  I L+GPCK PI I +QGT+ AP D+TG+GW+ F+Y+D  SLTGGGV
Sbjct: 61  SKLIIPKETYMLGVITLLGPCKSPIDIVVQGTIMAPVDITGDGWIQFRYVDKISLTGGGV 120

Query: 121 FDGQGKQAWENNDCDKNPSCARIPMSLKFSFITHSIVSDITSKDSKNFHVHVLGCNNLTL 180
           FDGQG++AWE NDC KNP CARIPMSLKFSF+  SIV DITSKDSKNFHV++LGC NLT 
Sbjct: 121 FDGQGQKAWERNDCHKNPKCARIPMSLKFSFVKDSIVRDITSKDSKNFHVNILGCKNLTF 180

Query: 181 QHVNINAPGNSINTDGIHIGRSIGINIINTNIGTGDDCISLGDGSKEVTITNVTCGPGHG 240
            HV INAPG SIN+DGIH+GR  G+NIIN+ IGTGDDCISLGDGS+EVTITNVTCGPGHG
Sbjct: 181 DHVTINAPGTSINSDGIHMGRCDGVNIINSVIGTGDDCISLGDGSREVTITNVTCGPGHG 240

Query: 241 ISVGSLGRYVVEEPVEGLRVKNCTLMNTSNGLRIKTWPASPAAGTASDMHYEDIIMVNVS 300
           ISVGSLG+Y+VEEPV GL VKNCTL+ T+NGLRIKTWPASPA G+ +DMHYEDI++V+V 
Sbjct: 241 ISVGSLGQYLVEEPVAGLYVKNCTLIGTTNGLRIKTWPASPATGSVTDMHYEDIVLVDVQ 300

Query: 301 NPILVDQEYCPYNKCNKEIPSKVKISKVSFKNIRGTSGNALAVKLVCSSSVPCEDVELAD 360
           NPI++DQEYCPYNKCN++IPSK+KISKVSFKNIRG++GNA+AVKL+CS+  PCEDV L+D
Sbjct: 301 NPIILDQEYCPYNKCNRKIPSKIKISKVSFKNIRGSAGNAVAVKLLCSADYPCEDVTLSD 360

Query: 361 IDLTYNGSEGPISSQCSNVKPTVTGKQNPPACSS 394
           ID+TY G EGP+ S+CSNVKP VTG Q P  C S
Sbjct: 361 IDITYTGREGPVVSECSNVKPVVTGIQKPIICPS 394

BLAST of Lsi08G003330 vs. TAIR 10
Match: AT3G07820.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 426.4 bits (1095), Expect = 2.5e-119
Identity = 202/368 (54.89%), Postives = 268/368 (72.83%), Query Frame = 0

Query: 30  DVRKYGAIPNADITQALKNAWKDACASTSQSTIIIPNVVYKLSAIELVGPCKGPIGIQLQ 89
           +V   G+   +DITQAL  A+  AC S+S S ++IP   +KL  IE+ GPCK PI + LQ
Sbjct: 23  EVFTIGSSSGSDITQALLKAFTSACQSSSPSKVVIPKGEFKLGEIEMRGPCKAPIEVTLQ 82

Query: 90  GTLQAPADLT--GEGWVHFKYIDHFSLTGGGVFDGQGKQAWENNDCDKNPSCARIPMSLK 149
           GT++A  +     E WV F  ID F L GGG FDG+G  AW  N+C K   C ++P+S++
Sbjct: 83  GTVKADGNAIQGKEKWVVFGNIDGFKLNGGGAFDGEGNAAWRVNNCHKTFECKKLPISIR 142

Query: 150 FSFITHSIVSDITSKDSKNFHVHVLGCNNLTLQHVNINAPGNSINTDGIHIGRSIGINII 209
           F FI +S + DI+S D+KNFH++VLG  N+T+ ++ I AP +S NTDGIH+GRS G+ I+
Sbjct: 143 FDFILNSEIRDISSIDAKNFHINVLGAKNMTMNNIKIVAPEDSPNTDGIHLGRSDGVKIL 202

Query: 210 NTNIGTGDDCISLGDGSKEVTITNVTCGPGHGISVGSLGRYVVEEPVEGLRVKNCTLMNT 269
           N+ I TGDDCIS+GDG K + +  VTCGPGHGISVGSLGRY  E+ V G++V NCTL  T
Sbjct: 203 NSFISTGDDCISVGDGMKNLHVEKVTCGPGHGISVGSLGRYGHEQDVSGIKVINCTLQET 262

Query: 270 SNGLRIKTWPASPAAGTASDMHYEDIIMVNVSNPILVDQEYCPYNKCNKEIPSKVKISKV 329
            NGLRIKTWP++  + TASD+H+EDII+ +VSNPIL+DQEYCP+N+CNK+  S +K+  +
Sbjct: 263 DNGLRIKTWPSAACSTTASDIHFEDIILKDVSNPILIDQEYCPWNQCNKQKASTIKLVNI 322

Query: 330 SFKNIRGTSGNALAVKLVCSSSVPCEDVELADIDLTYNGSEGPISSQCSNVKPTVTGKQN 389
           SFKNIRGTSGN  AVKL+CS   PC++VE+ DID+ YNG++GP +  CSNV P + G Q+
Sbjct: 323 SFKNIRGTSGNKDAVKLLCSKGYPCQNVEIGDIDIKYNGADGPATFHCSNVSPKILGSQS 382

Query: 390 PPACSSLA 396
           P ACS+ A
Sbjct: 383 PKACSAPA 390

BLAST of Lsi08G003330 vs. TAIR 10
Match: AT5G48140.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 422.2 bits (1084), Expect = 4.8e-118
Identity = 195/357 (54.62%), Postives = 263/357 (73.67%), Query Frame = 0

Query: 38  PNADITQALKNAWKDACASTSQSTIIIPNVVYKLSAIELVGPCKGPIGIQLQGTLQAPAD 97
           P +DIT AL  A+ +AC   ++ST++IP   YKL  I ++GPCK PI I L GT++A  +
Sbjct: 32  PGSDITNALLKAFNEACQFPTKSTVMIPKGEYKLGEIVMMGPCKAPIRIALLGTVKADGN 91

Query: 98  LTG-EGWVHFKYIDHFSLTGGGVFDGQGKQAWENNDCDKNPSCARIPMSLKFSFITHSIV 157
             G E WV F+ I+ F L GGGVFDG+G  AW  N+C K  +C ++P+S++F F+T + +
Sbjct: 92  ANGKEKWVAFRNINGFKLNGGGVFDGEGNAAWRVNNCHKTFNCKKLPISIRFDFVTDAKI 151

Query: 158 SDITSKDSKNFHVHVLGCNNLTLQHVNINAPGNSINTDGIHIGRSIGINIINTNIGTGDD 217
             ITS D+K+FH++V+G  N+T + V I AP  S NTDGIH+GRS GI IIN+ I TGDD
Sbjct: 152 RGITSLDAKHFHINVIGAKNVTFEDVKIIAPAESPNTDGIHVGRSDGIKIINSFISTGDD 211

Query: 218 CISLGDGSKEVTITNVTCGPGHGISVGSLGRYVVEEPVEGLRVKNCTLMNTSNGLRIKTW 277
           C+S+GDG K + +  VTCGPGHGIS+GSLGRY  EE V G+++ NCTL  T NGLRIKTW
Sbjct: 212 CVSVGDGMKNLLVERVTCGPGHGISIGSLGRYSHEENVSGIKIINCTLQETDNGLRIKTW 271

Query: 278 PASPAAGTASDMHYEDIIMVNVSNPILVDQEYCPYNKCNKEIPSKVKISKVSFKNIRGTS 337
           P++    TASD+H+E+I++ NVSNPIL+DQEYCP+N+CNK+ PS +K++ +SFK IRGTS
Sbjct: 272 PSAACTTTASDIHFENILLKNVSNPILIDQEYCPWNQCNKQKPSTIKLANISFKKIRGTS 331

Query: 338 GNALAVKLVCSSSVPCEDVELADIDLTYNGSEGPISSQCSNVKPTVTGKQNPPACSS 394
           GN  AVKL+CS   PC++VE+ D+++ Y G++GP + QCSNV P + G Q P ACSS
Sbjct: 332 GNKDAVKLLCSKGYPCQNVEVGDVNIQYTGADGPATFQCSNVSPKLVGTQIPKACSS 388

BLAST of Lsi08G003330 vs. TAIR 10
Match: AT3G07840.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 419.9 bits (1078), Expect = 2.4e-117
Identity = 202/386 (52.33%), Postives = 274/386 (70.98%), Query Frame = 0

Query: 10  LTFILLYVSIRKAQSSDTIFDVRKYGAIPNADITQALKNAWKDACASTSQSTIIIPNVVY 69
           ++ +L  +S+    ++  +F+V   G  P +DITQAL  A+  AC + + S ++I    +
Sbjct: 7   ISAVLFVISLLGFSANAEVFNV---GGSPGSDITQALLKAFTSACQAPTASKVVITKGEF 66

Query: 70  KLSAIELVGPCKGPIGIQLQGTLQA--PADLTGEGWVHFKYIDHFSLTGGGVFDGQGKQA 129
           KL  IE+ GPCK P+ I LQGTL+A   A    E WV F  I+ F L GGG+FDG+G  A
Sbjct: 67  KLGEIEMTGPCKAPVEINLQGTLKADGKAIQGKERWVVFLRINGFKLNGGGIFDGEGNAA 126

Query: 130 WENNDCDKNPSCARIPMSLKFSFITHSIVSDITSKDSKNFHVHVLGCNNLTLQHVNINAP 189
           W  N+C K   C ++P+S++F F+ ++ + DI+S D+KNFH++VLG  N+T  +V + AP
Sbjct: 127 WRVNNCHKTFECKKLPISIRFDFVENAEIRDISSIDAKNFHINVLGAKNMTFDNVKVIAP 186

Query: 190 GNSINTDGIHIGRSIGINIINTNIGTGDDCISLGDGSKEVTITNVTCGPGHGISVGSLGR 249
             S NTDGIH+GRS G+ I+N+ I TGDDCIS+GDG K + + NV CGPGHGISVGSLGR
Sbjct: 187 AESPNTDGIHLGRSEGVKILNSKIATGDDCISVGDGMKNLHVENVMCGPGHGISVGSLGR 246

Query: 250 YVVEEPVEGLRVKNCTLMNTSNGLRIKTWPASPAAGTASDMHYEDIIMVNVSNPILVDQE 309
           YV E+ V G+ V NCTL  T NGLRIKTWP++  A TAS +H+E+II+ NVSNPIL+DQE
Sbjct: 247 YVHEQDVTGITVVNCTLQGTDNGLRIKTWPSAACATTASGIHFENIILNNVSNPILIDQE 306

Query: 310 YCPYNKCNKEIPSKVKISKVSFKNIRGTSGNALAVKLVCSSSVPCEDVELADIDLTYNGS 369
           YCP+N+CNK+ PS +K+  +SFKNIRGTSGN  AVKL+CS + PC +VE+ +I+L Y G+
Sbjct: 307 YCPWNQCNKQKPSTIKLVDISFKNIRGTSGNKDAVKLLCSKAHPCANVEIGNINLEYKGA 366

Query: 370 EGPISSQCSNVKPTVTGKQNPPACSS 394
           +GP +  CSNV P + G QNP ACS+
Sbjct: 367 DGPPTFMCSNVSPKLVGTQNPKACSA 389

BLAST of Lsi08G003330 vs. TAIR 10
Match: AT3G07830.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 408.3 bits (1048), Expect = 7.2e-114
Identity = 190/373 (50.94%), Postives = 266/373 (71.31%), Query Frame = 0

Query: 30  DVRKYGAIPNADITQALKNAWKDACASTSQSTIIIPNVVYKLSAIELVGPCKGPIGIQLQ 89
           +V +  + P +DITQAL  A+  AC S +   ++IP   +KL  I + GPCK P+ I L 
Sbjct: 24  EVLRLTSSPGSDITQALLRAFTTACQSPTPRKVVIPKGQFKLGEIMMSGPCKSPVEITLL 83

Query: 90  GTLQAPADLT--GEGWVHFKYIDHFSLTGGGVFDGQGKQAWENNDCDKNPSCARIPMSLK 149
           GT+ A  +     E WV F+ +D F L GGG FDG+G  AW  N+C K   C ++P+S++
Sbjct: 84  GTVLADGNSIHGKEKWVVFQRMDGFRLNGGGTFDGEGNAAWRVNNCHKTFECKKLPISIR 143

Query: 150 FSFITHSIVSDITSKDSKNFHVHVLGCNNLTLQHVNINAPGNSINTDGIHIGRSIGINII 209
           F F+T++ + DI+S D+KNFH++V+G  N+T  +V I AP  S NTDGIH+GRS+G++II
Sbjct: 144 FDFVTNAEIRDISSIDAKNFHINVIGAKNMTFDNVKIMAPAESPNTDGIHLGRSVGVSII 203

Query: 210 NTNIGTGDDCISLGDGSKEVTITNVTCGPGHGISVGSLGRYVVEEPVEGLRVKNCTLMNT 269
           N+ I TGDDC+S+GDG   + + NV CGPGHGISVGSLGRY  E+ V G+RV NCTL  T
Sbjct: 204 NSRISTGDDCVSVGDGMVNLLVKNVVCGPGHGISVGSLGRYGHEQDVSGIRVINCTLQET 263

Query: 270 SNGLRIKTWPASPAAGTASDMHYEDIIMVNVSNPILVDQEYCPYNKCNKEIPSKVKISKV 329
            NGLRIKTWP++  + TAS++H+E+II+ NVSNPIL+DQEYCP+N+CNK+  S +K++ +
Sbjct: 264 DNGLRIKTWPSAACSTTASNIHFENIILRNVSNPILIDQEYCPWNQCNKQKSSSIKLANI 323

Query: 330 SFKNIRGTSGNALAVKLVCSSSVPCEDVELADIDLTYNGSEGPISSQCSNVKPTVTGKQN 389
           SF+ IRGTSGN  AVKL+CS   PCE+V++ DI++ Y G++GP +  CSNV+P + G Q 
Sbjct: 324 SFRRIRGTSGNKDAVKLLCSKGYPCENVQVGDINIQYTGADGPATFMCSNVRPKLVGTQF 383

Query: 390 PPACSSLALDSSP 401
           P AC++  + + P
Sbjct: 384 PKACNTPPVLTQP 396

BLAST of Lsi08G003330 vs. TAIR 10
Match: AT3G14040.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 390.6 bits (1002), Expect = 1.5e-108
Identity = 188/381 (49.34%), Postives = 263/381 (69.03%), Query Frame = 0

Query: 22  AQSSDTIFDVRKYGAIPN--ADITQALKNAWKDACASTSQSTIIIPNVVYKLSAIELVGP 81
           A+      DV+  GA  +   D + A   AWK+ACA  + STI +P   Y + ++E  GP
Sbjct: 64  AKPGGASLDVKASGAKGDGKTDDSAAFAAAWKEACA--AGSTITVPKGEYLVESLEFKGP 123

Query: 82  CKGPIGIQLQGTLQAPADL----TGEGWVHFKYIDHFSLTGG-GVFDGQGKQAWENNDCD 141
           CKGP+ ++L G  +APA +       GW+ F+ +  F+L G   +FDGQG  AW+ NDC 
Sbjct: 124 CKGPVTLELNGNFKAPATVKTTKPHAGWIDFENLADFTLNGNKAIFDGQGSLAWKANDCA 183

Query: 142 KNPSCARIPMSLKFSFITHSIVSDITSKDSKNFHVHVLGCNNLTLQHVNINAPGNSINTD 201
           K   C  +P++++F+ +T+S ++ ITS +SK FH+++L C N+TL  + I+AP  S+NTD
Sbjct: 184 KTGKCNSLPINIRFTGLTNSKINSITSTNSKLFHMNILNCKNITLTDIGIDAPPESLNTD 243

Query: 202 GIHIGRSIGINIINTNIGTGDDCISLGDGSKEVTITNVTCGPGHGISVGSLGRYVVEEPV 261
           GIHIGRS G+N+I   I TGDDC+S+GDG++ + + NV CGPGHGIS+GSLGRY  E+PV
Sbjct: 244 GIHIGRSNGVNLIGAKIKTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGRYPNEQPV 303

Query: 262 EGLRVKNCTLMNTSNGLRIKTWPASPAAGTASDMHYEDIIMVNVSNPILVDQEYCPYNKC 321
           +G+ V+ C + NT NG+RIKTWP SP  G AS++ +EDI M NVS P+L+DQEYCPY  C
Sbjct: 304 KGVTVRKCLIKNTDNGVRIKTWPGSP-PGIASNILFEDITMDNVSLPVLIDQEYCPYGHC 363

Query: 322 NKEIPSKVKISKVSFKNIRGTSGNALAVKLVCSSSVPCEDVELADIDLTYNGSEGPISSQ 381
              +PSKVK+S V+ KNI+GTS   +AVKL+CS  VPC ++ L+DI+L +NG EGP  S 
Sbjct: 364 KAGVPSKVKLSDVTIKNIKGTSATKVAVKLMCSKGVPCTNIALSDINLVHNGKEGPAVSA 423

Query: 382 CSNVKPTVTGKQNPPACSSLA 396
           CSN+KP ++GK  P AC+ +A
Sbjct: 424 CSNIKPILSGKLVPAACTEVA 441

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P245488.0e-12661.56Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1[more]
Q059675.0e-12053.08Polygalacturonase OS=Nicotiana tabacum OX=4097 GN=PG1 PE=2 SV=1[more]
Q397866.5e-12052.26Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1[more]
Q397661.1e-11952.26Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1[more]
Q403121.2e-11852.63Polygalacturonase OS=Medicago sativa OX=3879 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1DUC61.3e-18778.86exopolygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111024096 PE=3 SV... [more]
A0A6J1DBN71.5e-18377.31exopolygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111019009 PE=3 SV... [more]
A0A5A7T7B31.4e-17871.52Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A1S3CC084.2e-17877.92exopolygalacturonase-like OS=Cucumis melo OX=3656 GN=LOC103498752 PE=3 SV=1[more]
A0A6J1HJP45.1e-16872.08exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111465122 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022157387.12.7e-18778.86exopolygalacturonase-like [Momordica charantia] >XP_022159278.1 exopolygalacturo... [more]
XP_022150984.13.1e-18377.31exopolygalacturonase-like [Momordica charantia][more]
KAA0039364.13.0e-17871.52exopolygalacturonase-like [Cucumis melo var. makuwa] >TYK00547.1 exopolygalactur... [more]
XP_008459709.28.6e-17877.92PREDICTED: exopolygalacturonase-like [Cucumis melo][more]
XP_022965192.11.1e-16772.08exopolygalacturonase-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT3G07820.12.5e-11954.89Pectin lyase-like superfamily protein [more]
AT5G48140.14.8e-11854.62Pectin lyase-like superfamily protein [more]
AT3G07840.12.4e-11752.33Pectin lyase-like superfamily protein [more]
AT3G07830.17.2e-11450.94Pectin lyase-like superfamily protein [more]
AT3G14040.11.5e-10849.34Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 174..200
e-value: 200.0
score: 10.7
coord: 224..244
e-value: 2000.0
score: 5.1
coord: 254..275
e-value: 24.0
score: 13.8
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 54..380
e-value: 5.3E-99
score: 331.3
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 231..244
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 17..398
e-value: 1.4E-107
score: 362.3
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 13..395
NoneNo IPR availablePANTHERPTHR31375:SF248POLYGALACTURONASEcoord: 13..395
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 15..392

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi08G003330.1Lsi08G003330.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity