Lsi08G001690 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi08G001690
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionalpha/beta-Hydrolases superfamily protein
Locationchr08: 3503484 .. 3506800 (+)
RNA-Seq ExpressionLsi08G001690
SyntenyLsi08G001690
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACTTTTTTGATATGATTGGAGGAGCTGCAATATATAGAGGCTTAAAAGCAAACAAAAGTGACCTAAACTAAGTATACTCTGATCCATTTCATATTTTCCATAACTAAGTATACTCTGATCCATTTCATATTTTCCATAATAAAAAAGCCATGGAAAAGGTTCCATTAATATTCAGAGCGTTTCTGAGAGTCCAATCCGCCGCCTTGAACGTCAGTCTCCGGCCGAATAGGACTGTGAACCGTTTCATAATGAATCTCTTCGACCCAAAAATCCCTCCTTCTTCTAAACCAAACGACGGCGTTACAACTCACGACGTCGTTTTTGACTCAACTCGTAATCTATGGTTTCGCGTCTTTGTGCCTTGTTCAGACGATTCTGATAATCTTACCGGCCTTCCTCTGCTTGTGTACTTTCACGGTGGAGGCTTTTCGTTTCTTGCAGCTAATTCTATGCCTTTCGTTGACCATTGTCGGAGACTCGCACGTGACCTCCGTGTTGTGGTTGTTTCCGTCAACTATCGTCTCGCTCCTGAACATCAATACCCAATTCCCTATGAAGATGGATTTGATGCTTTGAAATTTCTTGACGATTTGGGATCTGATAATTCTGATGTTTTTCCGGTTAAAGTTGATTTTGATCGGTGTTTTCTCGCCGGTGATAGTGCCGGCGGAAATTTGGCCCACCACGTTGCAGTCAGAGCGGGCGGTTATCATTTCAAGAAGCTTAACATTAAAGGTTTGATCAGTAATCGATGCTATTCAAATGCTTTTTTTTTTATTAATTATTTTAAATGGCTCAAAAGAAGAGATATCTCCAATCCTAATATATAAATAATACCAATAAAGATTACTAAAAATATTTATAAATAAATAAAAATAAAGTACTTCTTTTATTTCATCCGAAAAGTCTTTTTCTTTTTAGTTCCACGGCCGATCAGTACCTAGCCGGGCTTTAACTCTTAAAAATATTTTAAATATAGCAAAATAACGCGAGAATATGTATTGATGTATATCTATTAGTATGTATCCCTTATCTATGGATAGATGTTTATAAGTATCTTATAAGTATCTATCATTATTTATCACAAACATACACTGATATTTTGCTATATTTTTAAATAAGTTGGCTCATTTTTTTTATATATTTAAATTTTTTTTTTAATGTTTTCTTCCTCCAAATGCTGCTTATTGTTTTAGTTTTGTACTTTCGGTTAATTTGAATTATATTATATTAAATTTAGTCTGTCAAAGTTAGTTTCTATATAAATTAGTTAAATAATAATAATAATTTTTATGTAAAAAAAATTTAATAGTGTGAATATGTTTTTAACATTTATAGTATAAATGTTAATAGATAATAAAAAAAATTTTAAAAGATCAATACATAATTTAAAATATATTAAAAATATAAAGATTAAAATTGAATAAACTTTTGAAAAGATAGATATTAAAATAATATTTTAGCCTTAATTTAATGTATTATTGATTCCACCTGTACATAGTTATTAAAATAACATTTTTGTGTTTTTTAAATTTAAATTTTCGAATTTGAAAATGTTGAATTACATCAGCATTGGTTTTTTATGAATATATTATTTTATGTTTTGGTTATAATTTTTAATTTAACCTTTTGTCATTTTGTATAATGTATTAAAATATTTGGTTAATAACTTTTTGTTAAAAGATTCTTATTATTTTGAAACTCGTTCAATTTTAATTTTATTATTGATTTTTTTATTATCTATTAGAATTTCTATAATAATTTTAAAAATGTATTCACATGAAAAAAAATATTATTATTATTTAACCAATTTTGATAAAAATAAATTTAGAGAAATTAATAAATTTTAAAATTTATTGAAAATATCAAGATTAAAATTGTAATTTTAAAGTATTTACAATAAAATTTAATAAACTCTAAACTATGTGAGACTAAAATGATACCTTTAACCTTTTATTTATTTTTTAATAAATATAACATTAAATGTTCTTGTTCTTTGAATAAGCTTGTGTACTTAACCATCAATGACTCCATAATAATTCGAATCATCCATCTCCTATTCTACATACCTAAAAATAAAAAAAAGAAGTGACATCTAATTTCTAATTCCGCTATCAAATATATATTAAAAACACTATAAACAATAAATATATTATATAATACTATATAGTGGAAGTCTGTTTTTAATTAAATTTCTTATTCTTACATCTATTTTGATATTATTAATTAATCCTCTCAATCTTAAGTTTAAAGGATTATTTTTAAATATAGAAAAATGAGTTAAAATATTTATAAATATAGAAAAATTTCATTGTCTACCAACGAAAACCATTATCGCTCAGTGATAGAAGTTTATTGTGGTCTATCGCTGATAACAGTGATAATTTTCTATATTTGTAACTAGTTTCATATTTTTTTCATTTATAATAATTTTTCAAATTTTAAAAGCATTATTAAATTACTTATTGTAAATCAAACTTAACACTTCATTCATGTTTTTAACTTTTTTTTTTTTTAGTAAGCTTAAAAATTAGCACAAAGTCATCAATAATTCTATATTGATATCATGTTAACTTAATTCTATATCAATATAATGTTTAACATTAATTTTATATTAATATTCAGATCAATTTATAAAGTTTTTATTAATAGGCTTAAATCACGGACCTCTTAGTCATTAACTTAAATTTTTTTTATTATTAATTAAGGTAAATTCAAATTAGTAACATTTCATTCCTATTATTTAACAAGGTTTAATTGCAATACAACCATTTTTCGGCGGGGAAGAGCGAGTAGAGTCGGAAGTTCAATACGGTAAGTCGGCAATGTTAAACTTGGAACAAGCGGATTGGTATTGGAAGGCATTTCTACCACATGGGTCCGACAGAAACCACCCAGCAGCACACGTGTACGGTCCCGACGCTGAAGACATCTCCGGGGTGAAGTTTCCGGCGACACTTCTGATCGTGGGAGGACAAGACCAGTTACAAGATTGGCAAAAAAGGTATTATGAGTGGCTGAAAGAGGCTGGAAAAGAAGTTGATTTGGTTGAATTTCCAGATGCTGTCCATGGATTTTATTTAGTTCCTGGGCTGGAGGATGATACTTCATTGTTCATCAAACATGCAAGTGAATTCATACTCAAACATATGGCAGTGTAAATTGGAAGATCTATATGGCTTTGCCTTGGATGTTTATTAGTGTGTGTTTTTAATTTGGCTTTCTAAGTGATAAGTTGCAATTTCATTCTTTGAATATTGATAAATTCTAAAAACATCTGTTCTCTAAAATTTGAATAAGAGTTTTGTTACCATCTA

mRNA sequence

ACTTTTTTGATATGATTGGAGGAGCTGCAATATATAGAGGCTTAAAAGCAAACAAAAGTGACCTAAACTAAGTATACTCTGATCCATTTCATATTTTCCATAACTAAGTATACTCTGATCCATTTCATATTTTCCATAATAAAAAAGCCATGGAAAAGGTTCCATTAATATTCAGAGCGTTTCTGAGAGTCCAATCCGCCGCCTTGAACGTCAGTCTCCGGCCGAATAGGACTGTGAACCGTTTCATAATGAATCTCTTCGACCCAAAAATCCCTCCTTCTTCTAAACCAAACGACGGCGTTACAACTCACGACGTCGTTTTTGACTCAACTCGTAATCTATGGTTTCGCGTCTTTGTGCCTTGTTCAGACGATTCTGATAATCTTACCGGCCTTCCTCTGCTTGTGTACTTTCACGGTGGAGGCTTTTCGTTTCTTGCAGCTAATTCTATGCCTTTCGTTGACCATTGTCGGAGACTCGCACGTGACCTCCGTGTTGTGGTTGTTTCCGTCAACTATCGTCTCGCTCCTGAACATCAATACCCAATTCCCTATGAAGATGGATTTGATGCTTTGAAATTTCTTGACGATTTGGGATCTGATAATTCTGATGTTTTTCCGGTTAAAGTTGATTTTGATCGGTGTTTTCTCGCCGGTGATAGTGCCGGCGGAAATTTGGCCCACCACGTTGCAGTCAGAGCGGGCGGTTATCATTTCAAGAAGCTTAACATTAAAGGTTTAATTGCAATACAACCATTTTTCGGCGGGGAAGAGCGAGTAGAGTCGGAAGTTCAATACGGTAAGTCGGCAATGTTAAACTTGGAACAAGCGGATTGGTATTGGAAGGCATTTCTACCACATGGGTCCGACAGAAACCACCCAGCAGCACACGTGTACGGTCCCGACGCTGAAGACATCTCCGGGGTGAAGTTTCCGGCGACACTTCTGATCGTGGGAGGACAAGACCAGTTACAAGATTGGCAAAAAAGGTATTATGAGTGGCTGAAAGAGGCTGGAAAAGAAGTTGATTTGGTTGAATTTCCAGATGCTGTCCATGGATTTTATTTAGTTCCTGGGCTGGAGGATGATACTTCATTGTTCATCAAACATGCAAGTGAATTCATACTCAAACATATGGCAGTGTAAATTGGAAGATCTATATGGCTTTGCCTTGGATGTTTATTAGTGTGTGTTTTTAATTTGGCTTTCTAAGTGATAAGTTGCAATTTCATTCTTTGAATATTGATAAATTCTAAAAACATCTGTTCTCTAAAATTTGAATAAGAGTTTTGTTACCATCTA

Coding sequence (CDS)

ATGGAAAAGGTTCCATTAATATTCAGAGCGTTTCTGAGAGTCCAATCCGCCGCCTTGAACGTCAGTCTCCGGCCGAATAGGACTGTGAACCGTTTCATAATGAATCTCTTCGACCCAAAAATCCCTCCTTCTTCTAAACCAAACGACGGCGTTACAACTCACGACGTCGTTTTTGACTCAACTCGTAATCTATGGTTTCGCGTCTTTGTGCCTTGTTCAGACGATTCTGATAATCTTACCGGCCTTCCTCTGCTTGTGTACTTTCACGGTGGAGGCTTTTCGTTTCTTGCAGCTAATTCTATGCCTTTCGTTGACCATTGTCGGAGACTCGCACGTGACCTCCGTGTTGTGGTTGTTTCCGTCAACTATCGTCTCGCTCCTGAACATCAATACCCAATTCCCTATGAAGATGGATTTGATGCTTTGAAATTTCTTGACGATTTGGGATCTGATAATTCTGATGTTTTTCCGGTTAAAGTTGATTTTGATCGGTGTTTTCTCGCCGGTGATAGTGCCGGCGGAAATTTGGCCCACCACGTTGCAGTCAGAGCGGGCGGTTATCATTTCAAGAAGCTTAACATTAAAGGTTTAATTGCAATACAACCATTTTTCGGCGGGGAAGAGCGAGTAGAGTCGGAAGTTCAATACGGTAAGTCGGCAATGTTAAACTTGGAACAAGCGGATTGGTATTGGAAGGCATTTCTACCACATGGGTCCGACAGAAACCACCCAGCAGCACACGTGTACGGTCCCGACGCTGAAGACATCTCCGGGGTGAAGTTTCCGGCGACACTTCTGATCGTGGGAGGACAAGACCAGTTACAAGATTGGCAAAAAAGGTATTATGAGTGGCTGAAAGAGGCTGGAAAAGAAGTTGATTTGGTTGAATTTCCAGATGCTGTCCATGGATTTTATTTAGTTCCTGGGCTGGAGGATGATACTTCATTGTTCATCAAACATGCAAGTGAATTCATACTCAAACATATGGCAGTGTAA

Protein sequence

MEKVPLIFRAFLRVQSAALNVSLRPNRTVNRFIMNLFDPKIPPSSKPNDGVTTHDVVFDSTRNLWFRVFVPCSDDSDNLTGLPLLVYFHGGGFSFLAANSMPFVDHCRRLARDLRVVVVSVNYRLAPEHQYPIPYEDGFDALKFLDDLGSDNSDVFPVKVDFDRCFLAGDSAGGNLAHHVAVRAGGYHFKKLNIKGLIAIQPFFGGEERVESEVQYGKSAMLNLEQADWYWKAFLPHGSDRNHPAAHVYGPDAEDISGVKFPATLLIVGGQDQLQDWQKRYYEWLKEAGKEVDLVEFPDAVHGFYLVPGLEDDTSLFIKHASEFILKHMAV
Homology
BLAST of Lsi08G001690 vs. ExPASy Swiss-Prot
Match: Q9LT10 (Probable carboxylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=CXE18 PE=1 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 1.7e-81
Identity = 148/320 (46.25%), Postives = 213/320 (66.56%), Query Frame = 0

Query: 4   VPLIFRAFLRVQSAALNVSLRPNRTVNRFIMNLFDPKIPPSSKPNDGVTTHDVVFDSTRN 63
           +PL  R  L V S   + + RP+ T+NR  + LFD + PP+ KP + V+T D V D +R+
Sbjct: 13  LPLKTRIALTVISTMTDNAQRPDGTINRRFLRLFDFRAPPNPKPVNIVSTSDFVVDQSRD 72

Query: 64  LWFRVFVP-CSDDSDNLTGLPLLVYFHGGGFSFLAANSMPFVDHCRRLARDLRVVVVSVN 123
           LWFR++ P  S D      +P++V+FHGGGF+FL+ N+ P+ + CRR AR L   V+SVN
Sbjct: 73  LWFRLYTPHVSGDK-----IPVVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPAYVISVN 132

Query: 124 YRLAPEHQYPIPYEDGFDALKFLDDLGSDNSDVFPVKVDFDRCFLAGDSAGGNLAHHVAV 183
           YRLAPEH+YP  Y+DGFDALK++++   ++  + P   D  RCF AGDSAGGN+AH+VA+
Sbjct: 133 YRLAPEHRYPAQYDDGFDALKYIEE---NHGSILPANADLSRCFFAGDSAGGNIAHNVAI 192

Query: 184 ---RAGGYHFKKLNIKGLIAIQPFFGGEERVESEVQYGKSAMLNLEQADWYWKAFLPHGS 243
              R     F  + + GLI+IQPFFGGEER E+E Q   + +++ ++ DW WKA    G 
Sbjct: 193 RICREPRSSFTAVKLIGLISIQPFFGGEERTEAEKQLVGAPLVSPDRTDWCWKAM---GL 252

Query: 244 DRNHPAAHVYGPDAEDISGVKFPATLLIVGGQDQLQDWQKRYYEWLKEAGKEVDLVEFPD 303
           +R+H A +V GP+A DISG+ +P T+++V G D L+DWQ+ YYEWLK  GK+  L+E+P+
Sbjct: 253 NRDHEAVNVGGPNAVDISGLDYPETMVVVAGFDPLKDWQRSYYEWLKLCGKKATLIEYPN 312

Query: 304 AVHGFYLVPGLEDDTSLFIK 320
             H FY+ P L +   L ++
Sbjct: 313 MFHAFYIFPELPEAGQLIMR 321

BLAST of Lsi08G001690 vs. ExPASy Swiss-Prot
Match: Q940G6 (Gibberellin receptor GID1C OS=Arabidopsis thaliana OX=3702 GN=GID1C PE=1 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 6.0e-63
Identity = 130/315 (41.27%), Postives = 185/315 (58.73%), Query Frame = 0

Query: 4   VPLIFRAFLRVQSAALNVSLRPNRTVNRFIMNLFDPKIPPSSKPNDGVTTHDVVFDSTRN 63
           VPL     +     A N+  RP+ T NR +    D K+P ++ P +GV + DV+ D   N
Sbjct: 16  VPLNTWVLISNFKLAYNLLRRPDGTFNRHLAEFLDRKVPANANPVNGVFSFDVIIDRQTN 75

Query: 64  LWFRVFVPC-SDDSDNLTGL---------PLLVYFHGGGFSFLAANSMPFVDHCRRLARD 123
           L  RV+ P  +  S ++T L         P++V+FHGG F+  +ANS  +   CRRL   
Sbjct: 76  LLSRVYRPADAGTSPSITDLQNPVDGEIVPVIVFFHGGSFAHSSANSAIYDTLCRRLVGL 135

Query: 124 LRVVVVSVNYRLAPEHQYPIPYEDGFDALKFLDDLGSDNSDVFPVKVDFDRCFLAGDSAG 183
              VVVSVNYR APE++YP  Y+DG+  LK++    + +S +   K    R FLAGDS+G
Sbjct: 136 CGAVVVSVNYRRAPENRYPCAYDDGWAVLKWV----NSSSWLRSKKDSKVRIFLAGDSSG 195

Query: 184 GNLAHHVAVRAGGYHFKKLNIKGLIAIQPFFGGEERVESEVQYGKSAMLNLEQADWYWKA 243
           GN+ H+VAVRA      ++++ G I + P FGG ER ESE +      + +   DWYW+A
Sbjct: 196 GNIVHNVAVRAVE---SRIDVLGNILLNPMFGGTERTESEKRLDGKYFVTVRDRDWYWRA 255

Query: 244 FLPHGSDRNHPAAHVYGPDAEDISGVKFPATLLIVGGQDQLQDWQKRYYEWLKEAGKEVD 303
           FLP G DR HPA   +GP ++ + G+ FP +L++V G D +QDWQ +Y E LK+AG+EV 
Sbjct: 256 FLPEGEDREHPACSPFGPRSKSLEGLSFPKSLVVVAGLDLIQDWQLKYAEGLKKAGQEVK 315

Query: 304 LVEFPDAVHGFYLVP 309
           L+    A  GFYL+P
Sbjct: 316 LLYLEQATIGFYLLP 323

BLAST of Lsi08G001690 vs. ExPASy Swiss-Prot
Match: Q9MAA7 (Gibberellin receptor GID1A OS=Arabidopsis thaliana OX=3702 GN=GID1A PE=1 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 3.3e-61
Identity = 130/333 (39.04%), Postives = 183/333 (54.95%), Query Frame = 0

Query: 4   VPLIFRAFLRVQSAALNVSLRPNRTVNRFIMNLFDPKIPPSSKPNDGVTTHDVVFDSTRN 63
           VPL     +     A N+  RP+ T NR +    D K+  ++ P DGV + DV+ D   N
Sbjct: 16  VPLNTWVLISNFKVAYNILRRPDGTFNRHLAEYLDRKVTANANPVDGVFSFDVLIDRRIN 75

Query: 64  LWFRVFVPCSDDSDNLTGL------------PLLVYFHGGGFSFLAANSMPFVDHCRRLA 123
           L  RV+ P   D +    +            P++++FHGG F+  +ANS  +   CRRL 
Sbjct: 76  LLSRVYRPAYADQEQPPSILDLEKPVDGDIVPVILFFHGGSFAHSSANSAIYDTLCRRLV 135

Query: 124 RDLRVVVVSVNYRLAPEHQYPIPYEDGFDALKFLDDLGSDNSDVFPVKVDFDRCFLAGDS 183
              + VVVSVNYR APE+ YP  Y+DG+ AL ++    +  S +   K      FLAGDS
Sbjct: 136 GLCKCVVVSVNYRRAPENPYPCAYDDGWIALNWV----NSRSWLKSKKDSKVHIFLAGDS 195

Query: 184 AGGNLAHHVAVRAGGYHFKKLNIKGLIAIQPFFGGEERVESEVQYGKSAMLNLEQADWYW 243
           +GGN+AH+VA+RAG      +++ G I + P FGG ER ESE        + +   DWYW
Sbjct: 196 SGGNIAHNVALRAGE---SGIDVLGNILLNPMFGGNERTESEKSLDGKYFVTVRDRDWYW 255

Query: 244 KAFLPHGSDRNHPAAHVYGPDAEDISGVKFPATLLIVGGQDQLQDWQKRYYEWLKEAGKE 303
           KAFLP G DR HPA + + P  + + GV FP +L++V G D ++DWQ  Y E LK+AG+E
Sbjct: 256 KAFLPEGEDREHPACNPFSPRGKSLEGVSFPKSLVVVAGLDLIRDWQLAYAEGLKKAGQE 315

Query: 304 VDLVEFPDAVHGFYLVP------GLEDDTSLFI 319
           V L+    A  GFYL+P       + D+ S F+
Sbjct: 316 VKLMHLEKATVGFYLLPNNNHFHNVMDEISAFV 341

BLAST of Lsi08G001690 vs. ExPASy Swiss-Prot
Match: Q9LYC1 (Gibberellin receptor GID1B OS=Arabidopsis thaliana OX=3702 GN=GID1B PE=1 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 1.1e-59
Identity = 130/339 (38.35%), Postives = 188/339 (55.46%), Query Frame = 0

Query: 4   VPLIFRAFLRVQSAALNVSLRPNRTVNRFIMNLFDPKIPPSSKPNDGVTTHDVVFDSTRN 63
           VPL     +     A  V  RP+ + NR +    D K+P +S P DGV + D V DST N
Sbjct: 16  VPLNTWVLISNFKLAYKVLRRPDGSFNRDLAEFLDRKVPANSFPLDGVFSFDHV-DSTTN 75

Query: 64  LWFRVFVPCSDDSDNLTG-------------LPLLVYFHGGGFSFLAANSMPFVDHCRRL 123
           L  R++ P S       G             +P+L++FHGG F+  +ANS  +   CRRL
Sbjct: 76  LLTRIYQPASLLHQTRHGTLELTKPLSTTEIVPVLIFFHGGSFTHSSANSAIYDTFCRRL 135

Query: 124 ARDLRVVVVSVNYRLAPEHQYPIPYEDGFDALKFLDD----LGSDNSDVFPVKVDFDRCF 183
                VVVVSV+YR +PEH+YP  Y+DG++AL ++          +S+V+         +
Sbjct: 136 VTICGVVVVSVDYRRSPEHRYPCAYDDGWNALNWVKSRVWLQSGKDSNVY--------VY 195

Query: 184 LAGDSAGGNLAHHVAVRAGGYHFKKLNIKGLIAIQPFFGGEERVESEVQYGKSAMLNLEQ 243
           LAGDS+GGN+AH+VAVRA     K L   G I + P FGG+ER +SE        + ++ 
Sbjct: 196 LAGDSSGGNIAHNVAVRATNEGVKVL---GNILLHPMFGGQERTQSEKTLDGKYFVTIQD 255

Query: 244 ADWYWKAFLPHGSDRNHPAAHVYGPDAEDISGVKFPATLLIVGGQDQLQDWQKRYYEWLK 303
            DWYW+A+LP G DR+HPA + +GP  + + GV FP +L++V G D +QDWQ  Y + LK
Sbjct: 256 RDWYWRAYLPEGEDRDHPACNPFGPRGQSLKGVNFPKSLVVVAGLDLVQDWQLAYVDGLK 315

Query: 304 EAGKEVDLVEFPDAVHGFYLVPGLEDDTSLFIKHASEFI 326
           + G EV+L+    A  GFY +P   D     ++  ++F+
Sbjct: 316 KTGLEVNLLYLKQATIGFYFLPN-NDHFHCLMEELNKFV 341

BLAST of Lsi08G001690 vs. ExPASy Swiss-Prot
Match: Q6L545 (Gibberellin receptor GID1 OS=Oryza sativa subsp. japonica OX=39947 GN=GID1 PE=1 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 9.0e-51
Identity = 114/329 (34.65%), Postives = 174/329 (52.89%), Query Frame = 0

Query: 4   VPLIFRAFLRVQSAALNVSLRPNRTVNRFIMNLFDPKIPPSSKPNDGVTTHDVVFDSTRN 63
           VPL     +     + N+  R + T  R +    D ++P +++P +GV++ D + D +  
Sbjct: 16  VPLHTWVLISNFKLSYNILRRADGTFERDLGEYLDRRVPANARPLEGVSSFDHIIDQSVG 75

Query: 64  LWFRVFVPCSD-------------------DSDNLTGLPLLVYFHGGGFSFLAANSMPFV 123
           L  R++   ++                   D+      P++++FHGG F   +A+S  + 
Sbjct: 76  LEVRIYRAAAEGDAEEGAAAVTRPILEFLTDAPAAEPFPVIIFFHGGSFVHSSASSTIYD 135

Query: 124 DHCRRLARDLRVVVVSVNYRLAPEHQYPIPYEDGFDALKFLDD-----LGSDNSDVFPVK 183
             CRR  +  + VVVSVNYR APEH+YP  Y+DG+ ALK++        G D        
Sbjct: 136 SLCRRFVKLSKGVVVSVNYRRAPEHRYPCAYDDGWTALKWVMSQPFMRSGGDAQ------ 195

Query: 184 VDFDRCFLAGDSAGGNLAHHVAVRAGGYHFKKLNIKGLIAIQPFFGGEERVESEVQYGKS 243
               R FL+GDS+GGN+AHHVAVRA     + + + G I +   FGG ER ESE +    
Sbjct: 196 ---ARVFLSGDSSGGNIAHHVAVRAAD---EGVKVCGNILLNAMFGGTERTESERRLDGK 255

Query: 244 AMLNLEQADWYWKAFLPHGSDRNHPAAHVYGPDAEDISGVKFPATLLIVGGQDQLQDWQK 303
             + L+  DWYWKA+LP  +DR+HPA + +GP+   + G+ F  +L+IV G D   D Q 
Sbjct: 256 YFVTLQDRDWYWKAYLPEDADRDHPACNPFGPNGRRLGGLPFAKSLIIVSGLDLTCDRQL 315

Query: 304 RYYEWLKEAGKEVDLVEFPDAVHGFYLVP 309
            Y + L+E G  V +V+  +A  GFYL+P
Sbjct: 316 AYADALREDGHHVKVVQCENATVGFYLLP 332

BLAST of Lsi08G001690 vs. ExPASy TrEMBL
Match: A0A6J1DXC5 (probable carboxylesterase 18 OS=Momordica charantia OX=3673 GN=LOC111023986 PE=4 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 1.4e-131
Identity = 230/329 (69.91%), Postives = 271/329 (82.37%), Query Frame = 0

Query: 3   KVPLIFRAFLRVQSAALNVSLRPNRTVNRFIMNLFDPKIPPSSKPNDGVTTHDVVFDSTR 62
           K+P  FR  +R+ +AA  VS R N TVNR +M+LFDPK PPS+KP  GV++HDVVFD   
Sbjct: 12  KLPWKFRMLVRLNAAASYVSRRSNMTVNRRLMSLFDPKTPPSTKPRHGVSSHDVVFDPNX 71

Query: 63  NLWFRVFVPCSDDSDNLTGLPLLVYFHGGGFSFLAANSMPFVDHCRRLARDLRVVVVSVN 122
           NLWFR+F+P S   DNLT +P++VY+HGGGF F +ANS P+ D CRRLARDLR VVVSVN
Sbjct: 72  NLWFRLFIP-SAVPDNLT-VPVVVYYHGGGFVFFSANSQPYDDLCRRLARDLRAVVVSVN 131

Query: 123 YRLAPEHQYPIPYEDGFDALKFLDDLGSDNSDVFPVKVDFDRCFLAGDSAGGNLAHHVAV 182
           YRL+PEH+YP PYEDGFDA+KFLD +G D+   FPVK D  RCFLAGDSAGGNLAHHVAV
Sbjct: 132 YRLSPEHRYPCPYEDGFDAVKFLDXVGFDSLS-FPVKADLGRCFLAGDSAGGNLAHHVAV 191

Query: 183 RAGGYHFKKLNIKGLIAIQPFFGGEERVESEVQYGKSAMLNLEQADWYWKAFLPHGSDRN 242
           RA  Y F K+NIKGLI I+PFFGGEERVESE+Q+ +S M+NLEQADWYWKAFLP+GS+RN
Sbjct: 192 RAADYDFNKVNIKGLITIEPFFGGEERVESEIQFSESPMVNLEQADWYWKAFLPNGSNRN 251

Query: 243 HPAAHVYGPDAEDISGVKFPATLLIVGGQDQLQDWQKRYYEWLKEAGKEVDLVEFPDAVH 302
           HPAAHV+GPDA DISGVKFPA LLIVGG DQL+DW ++YYEWLK AGKEVDLVE+P A+H
Sbjct: 252 HPAAHVFGPDAGDISGVKFPAXLLIVGGHDQLRDWGRKYYEWLKGAGKEVDLVEYPKAIH 311

Query: 303 GFYLVPGLEDDTSLFIKHASEFILKHMAV 332
           GFY VP L+ D SL IK A++F+ K +AV
Sbjct: 312 GFYAVPQLK-DASLLIKDANDFMQKQLAV 336

BLAST of Lsi08G001690 vs. ExPASy TrEMBL
Match: A0A6J1I994 (probable carboxylesterase 18 OS=Cucurbita maxima OX=3661 GN=LOC111472693 PE=4 SV=1)

HSP 1 Score: 478.0 bits (1229), Expect = 3.2e-131
Identity = 229/325 (70.46%), Postives = 266/325 (81.85%), Query Frame = 0

Query: 3   KVPLIFRAFLRVQSAALNVSLRPNRTVNRFIMNLFDPKIPPSSKPNDGVTTHDVVFDSTR 62
           K+PL F+  LR+ S A ++  RPN TVNR + NLFDPK  PSSKPN+GV+THD+VFD +R
Sbjct: 6   KLPLKFQILLRLHSFASSIGRRPNITVNRCLTNLFDPKFSPSSKPNNGVSTHDIVFDPSR 65

Query: 63  NLWFRVFVPCSDDSDNLTGLPLLVYFHGGGFSFLAANSMPFVDHCRRLARDLRVVVVSVN 122
           NLWFR+FVP S   D    LP++VYFHGGGF F +ANSMP+ D  RRLARDLRVVVVSVN
Sbjct: 66  NLWFRLFVPSSVSDD--VSLPVVVYFHGGGFVFFSANSMPYDDLGRRLARDLRVVVVSVN 125

Query: 123 YRLAPEHQYPIPYEDGFDALKFLDDLGSDNSDVFPVKVDFDRCFLAGDSAGGNLAHHVAV 182
           YRL+PEH+YP+PYEDGFD LKFLD++G D S VFP K D  RCFLAGDSAGGNLAHHVAV
Sbjct: 126 YRLSPEHRYPLPYEDGFDGLKFLDEMGFD-SVVFPAKADLGRCFLAGDSAGGNLAHHVAV 185

Query: 183 RAGGYHFKKLNIKGLIAIQPFFGGEERVESEVQYGKSAMLNLEQADWYWKAFLPHGSDRN 242
           RA GY FKK+NIKGLIAIQPFFGGEER  SEVQ+ KS ML+LEQADWYWKAFLP GS+RN
Sbjct: 186 RAAGYDFKKVNIKGLIAIQPFFGGEERTNSEVQFSKSPMLSLEQADWYWKAFLPDGSNRN 245

Query: 243 HPAAHVYGPDAEDISGVKFPATLLIVGGQDQLQDWQKRYYEWLKEAGKEVDLVEFPDAVH 302
           HPAAHV+G  A D+ G KFP TLLIVGG+DQL+DW  +YYEWLKE+ KEVDLVE+P+A+H
Sbjct: 246 HPAAHVFGAGAGDLPGEKFPTTLLIVGGRDQLRDWGMKYYEWLKESDKEVDLVEYPNAIH 305

Query: 303 GFYLVPGLEDDTSLFIKHASEFILK 328
           GFY VP L+ D SL IK  ++F+ K
Sbjct: 306 GFYAVPQLK-DCSLLIKDVNDFMQK 326

BLAST of Lsi08G001690 vs. ExPASy TrEMBL
Match: A0A1S3CAV2 (probable carboxylesterase 18 OS=Cucumis melo OX=3656 GN=LOC103498628 PE=4 SV=1)

HSP 1 Score: 474.9 bits (1221), Expect = 2.7e-130
Identity = 230/330 (69.70%), Postives = 271/330 (82.12%), Query Frame = 0

Query: 1   MEKVPLIFRAFLRVQSAALNVSLRPNRTVNRFIMNLFDPKIPPSSKPNDGVTTHDVVFDS 60
           MEK+PL FR  L   S A ++S R N TVNRF M+ FDPK  PSSKP +GV+T+DVVFD 
Sbjct: 1   MEKLPLKFRILLHFHSFASSISRRSNVTVNRFFMSFFDPKFSPSSKPRNGVSTYDVVFDP 60

Query: 61  TRNLWFRVFVPCSDDSD-NLTGLPLLVYFHGGGFSFLAANSMPFVDHCRRLARDLRVVVV 120
           + NLWFR+F+P S  +D N+T LP++VY+HGGGF F +ANSM + D CRRLAR+LRV VV
Sbjct: 61  SHNLWFRLFLPSSSSADKNVTDLPVVVYYHGGGFVFFSANSMAYDDFCRRLARELRVAVV 120

Query: 121 SVNYRLAPEHQYPIPYEDGFDALKFLDDLGSDNSDVFPVKVDFDRCFLAGDSAGGNLAHH 180
           SVNYRL+PEH+ PIPYEDGFDALKFLD +  D+   FPVK D  RCFLAGDSAGGNL HH
Sbjct: 121 SVNYRLSPEHRCPIPYEDGFDALKFLDGMDLDDGG-FPVKFDVSRCFLAGDSAGGNLTHH 180

Query: 181 VAVRAGGYHFKKLNIKGLIAIQPFFGGEERVESEVQYGKSAMLNLEQADWYWKAFLPHGS 240
           VAVRAGGY+FKKL IKG+IAIQPFFGGEERVESE+++ KS MLNL+Q+DWYWKAFLP G 
Sbjct: 181 VAVRAGGYNFKKLKIKGIIAIQPFFGGEERVESEIKFSKSPMLNLKQSDWYWKAFLPKGC 240

Query: 241 DRNHPAAHVYGPDA-EDISGVKFPATLLIVGGQDQLQDWQKRYYEWLK-EAGKEVDLVEF 300
           DRNHPAAHV+GP A +DIS VKFP TLLI+GG+DQL DW KRYYEWLK E GKEVDLVE+
Sbjct: 241 DRNHPAAHVFGPGAGDDISKVKFPTTLLILGGKDQLVDWGKRYYEWLKDECGKEVDLVEY 300

Query: 301 PDAVHGFYLVPGLEDDTSLFIKHASEFILK 328
           P+A+HGFY+VP L+ D+SL IK  ++FI K
Sbjct: 301 PNAIHGFYIVPELK-DSSLLIKDMNDFIHK 328

BLAST of Lsi08G001690 vs. ExPASy TrEMBL
Match: A0A6J1EW52 (probable carboxylesterase 18 OS=Cucurbita moschata OX=3662 GN=LOC111436652 PE=4 SV=1)

HSP 1 Score: 465.3 bits (1196), Expect = 2.1e-127
Identity = 224/329 (68.09%), Postives = 262/329 (79.64%), Query Frame = 0

Query: 3   KVPLIFRAFLRVQSAALNVSLRPNRTVNRFIMNLFDPKIPPSSKPNDGVTTHDVVFDSTR 62
           K+PL F+  LR+ S   ++  RPN TVNR + NLFD K  PSSKPN+GV+T D+VFD  R
Sbjct: 6   KLPLKFQVLLRLHSFVTSIGRRPNITVNRCLTNLFDLKFSPSSKPNNGVSTRDIVFDPAR 65

Query: 63  NLWFRVFVPCSDDSDNLTGLPLLVYFHGGGFSFLAANSMPFVDHCRRLARDLRVVVVSVN 122
           NLWFR+FVP     D    LP++VYFHGGGF F +ANSMP+ D  RRLARDLRVVVVSVN
Sbjct: 66  NLWFRLFVPSLVSDD--VSLPVVVYFHGGGFVFFSANSMPYDDLGRRLARDLRVVVVSVN 125

Query: 123 YRLAPEHQYPIPYEDGFDALKFLDDLGSDNSDVFPVKVDFDRCFLAGDSAGGNLAHHVAV 182
           YRL+PEH+ P+PYEDGFD LKFLD++G D S VFP K D  RCFLAGDSAGGNLAHHVAV
Sbjct: 126 YRLSPEHRCPLPYEDGFDGLKFLDEMGFD-SVVFPAKADLGRCFLAGDSAGGNLAHHVAV 185

Query: 183 RAGGYHFKKLNIKGLIAIQPFFGGEERVESEVQYGKSAMLNLEQADWYWKAFLPHGSDRN 242
           RA GY FKK+NIKGLIAIQPFFGGEER  SEVQ+ KS ML+LEQADWYWKAFLP GS+RN
Sbjct: 186 RAAGYDFKKVNIKGLIAIQPFFGGEERTNSEVQFSKSPMLSLEQADWYWKAFLPDGSNRN 245

Query: 243 HPAAHVYGPDAEDISGVKFPATLLIVGGQDQLQDWQKRYYEWLKEAGKEVDLVEFPDAVH 302
           HPAAHV+GPDA D+    FP TLLIVGG+DQL+DW  +YYEWLKE+ KEV+LVE+P+A+H
Sbjct: 246 HPAAHVFGPDARDLPAENFPTTLLIVGGRDQLRDWGMKYYEWLKESDKEVNLVEYPNAIH 305

Query: 303 GFYLVPGLEDDTSLFIKHASEFILKHMAV 332
           GFY VP L+ D SL I+  ++FI K + V
Sbjct: 306 GFYAVPQLK-DCSLLIRDVNDFIQKCLLV 330

BLAST of Lsi08G001690 vs. ExPASy TrEMBL
Match: A0A0A0KUP0 (Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G647320 PE=4 SV=1)

HSP 1 Score: 461.8 bits (1187), Expect = 2.3e-126
Identity = 225/332 (67.77%), Postives = 269/332 (81.02%), Query Frame = 0

Query: 1   MEKVPLIFRAFLRVQSAALNVSLRPNRTVNRFIMNLFDPKIPPSSKPNDGVTTHDVVFDS 60
           ME++P  FR  L   S A  +S R N TVNRF+M+LFDPK   SSKP  GV+T+DVVFD 
Sbjct: 1   MERLPFKFRILLYFFSFASFISRRSNVTVNRFLMSLFDPKYSASSKPRHGVSTYDVVFDP 60

Query: 61  TRNLWFRVFVPCSD---DSDNLTGLPLLVYFHGGGFSFLAANSMPFVDHCRRLARDLRVV 120
           + NLWFR+F+P S    D++N+T LP++VY+HGGGF F +ANSM + D CRRLAR+LRV 
Sbjct: 61  SHNLWFRLFLPSSSSSTDNNNVTDLPVIVYYHGGGFVFFSANSMAYDDLCRRLARELRVA 120

Query: 121 VVSVNYRLAPEHQYPIPYEDGFDALKFLDDLGSDNSDVFPVKVDFDRCFLAGDSAGGNLA 180
           VVSVNYRL+PEH+ PIPYEDGFDALK+LD +  D    FPVK+D  RCFLAGDSAGGNLA
Sbjct: 121 VVSVNYRLSPEHRCPIPYEDGFDALKYLDGMDLDGGG-FPVKLDVSRCFLAGDSAGGNLA 180

Query: 181 HHVAVRAGGYHFKKLNIKGLIAIQPFFGGEERVESEVQYGKSAMLNLEQADWYWKAFLPH 240
           HHVAVRAGG++FKKL IKG+IAIQPFFGGEERVESE+++ KS MLNLEQADWYWKAFLP 
Sbjct: 181 HHVAVRAGGHNFKKLKIKGIIAIQPFFGGEERVESEIKFSKSPMLNLEQADWYWKAFLPK 240

Query: 241 GSDRNHPAAHVYGPD-AEDISGVKFPATLLIVGGQDQLQDWQKRYYEWLK-EAGKEVDLV 300
           G DRNHPA HV+GP   ++IS VKFP TLLI+GG+DQL DW K+YYEWLK E GKEVDLV
Sbjct: 241 GCDRNHPAVHVFGPSGGDEISKVKFPTTLLILGGKDQLGDWGKKYYEWLKDECGKEVDLV 300

Query: 301 EFPDAVHGFYLVPGLEDDTSLFIKHASEFILK 328
           E+P+A+HGFY+VP L+ D+SL IK  ++FI K
Sbjct: 301 EYPNAIHGFYVVPELK-DSSLLIKDMNDFIHK 330

BLAST of Lsi08G001690 vs. NCBI nr
Match: XP_038890672.1 (probable carboxylesterase 18 [Benincasa hispida])

HSP 1 Score: 496.9 bits (1278), Expect = 1.4e-136
Identity = 239/331 (72.21%), Postives = 279/331 (84.29%), Query Frame = 0

Query: 1   MEKVPLIFRAFLRVQSAALNVSLRPNRTVNRFIMNLFDPKIPPSSKPNDGVTTHDVVFDS 60
           MEK+PL FR  LR++S A  +S RPN TVNRF+M+ FDPK PPSSKP +GV+T+DVVFD 
Sbjct: 3   MEKLPLKFRILLRLRSIASFISRRPNITVNRFLMSFFDPKTPPSSKPRNGVSTNDVVFDP 62

Query: 61  TRNLWFRVFVPCSDDSDNLTGLPLLVYFHGGGFSFLAANSMPFVDHCRRLARDLRVVVVS 120
            RNLWFR+F+P S    +   LP++VY+HGGGF F +ANSMP+ D CRRLARDLRVVVVS
Sbjct: 63  FRNLWFRLFLPSSSVLADNVSLPVVVYYHGGGFVFFSANSMPYDDFCRRLARDLRVVVVS 122

Query: 121 VNYRLAPEHQYPIPYEDGFDALKFLDDLGSDNSDVFPVKVDFDRCFLAGDSAGGNLAHHV 180
           VNYRL+PEH+YPIPYEDGFDALKFLD  G+D   VFP  VDFD+CF+AGDSAGGNLAH V
Sbjct: 123 VNYRLSPEHRYPIPYEDGFDALKFLD--GTDL--VFPANVDFDQCFIAGDSAGGNLAHQV 182

Query: 181 AVRAGGYHFKKLNIKGLIAIQPFFGGEERVESEVQYGKSAMLNLEQADWYWKAFLPHGSD 240
           AV+AGGY FKKL IKGLIAIQPFFGGEERVESE+++GKS ML LE+ADW+WKAFLP+GS+
Sbjct: 183 AVKAGGYDFKKLKIKGLIAIQPFFGGEERVESEIRFGKSPMLTLEEADWFWKAFLPNGSN 242

Query: 241 RNHPAAHVYGPDAEDISGVKFPATLLIVGGQDQLQDWQKRYYEWLKEAGKEVDLVEFPDA 300
           RNHPAAHV+GP A DIS VKFPATLLIVGG+DQL DW K+YYEWLKE+GKEVDLVE+P+A
Sbjct: 243 RNHPAAHVFGPRAGDISDVKFPATLLIVGGKDQLLDWGKKYYEWLKESGKEVDLVEYPNA 302

Query: 301 VHGFYLVPGLEDDTSLFIKHASEFILKHMAV 332
           +HGFY VP L+ D SL IK   +F+ K MAV
Sbjct: 303 IHGFYAVPELK-DASLLIKDVGDFMHKLMAV 328

BLAST of Lsi08G001690 vs. NCBI nr
Match: XP_022157226.1 (probable carboxylesterase 18 [Momordica charantia])

HSP 1 Score: 479.2 bits (1232), Expect = 2.9e-131
Identity = 230/329 (69.91%), Postives = 271/329 (82.37%), Query Frame = 0

Query: 3   KVPLIFRAFLRVQSAALNVSLRPNRTVNRFIMNLFDPKIPPSSKPNDGVTTHDVVFDSTR 62
           K+P  FR  +R+ +AA  VS R N TVNR +M+LFDPK PPS+KP  GV++HDVVFD   
Sbjct: 12  KLPWKFRMLVRLNAAASYVSRRSNMTVNRRLMSLFDPKTPPSTKPRHGVSSHDVVFDPNX 71

Query: 63  NLWFRVFVPCSDDSDNLTGLPLLVYFHGGGFSFLAANSMPFVDHCRRLARDLRVVVVSVN 122
           NLWFR+F+P S   DNLT +P++VY+HGGGF F +ANS P+ D CRRLARDLR VVVSVN
Sbjct: 72  NLWFRLFIP-SAVPDNLT-VPVVVYYHGGGFVFFSANSQPYDDLCRRLARDLRAVVVSVN 131

Query: 123 YRLAPEHQYPIPYEDGFDALKFLDDLGSDNSDVFPVKVDFDRCFLAGDSAGGNLAHHVAV 182
           YRL+PEH+YP PYEDGFDA+KFLD +G D+   FPVK D  RCFLAGDSAGGNLAHHVAV
Sbjct: 132 YRLSPEHRYPCPYEDGFDAVKFLDXVGFDSLS-FPVKADLGRCFLAGDSAGGNLAHHVAV 191

Query: 183 RAGGYHFKKLNIKGLIAIQPFFGGEERVESEVQYGKSAMLNLEQADWYWKAFLPHGSDRN 242
           RA  Y F K+NIKGLI I+PFFGGEERVESE+Q+ +S M+NLEQADWYWKAFLP+GS+RN
Sbjct: 192 RAADYDFNKVNIKGLITIEPFFGGEERVESEIQFSESPMVNLEQADWYWKAFLPNGSNRN 251

Query: 243 HPAAHVYGPDAEDISGVKFPATLLIVGGQDQLQDWQKRYYEWLKEAGKEVDLVEFPDAVH 302
           HPAAHV+GPDA DISGVKFPA LLIVGG DQL+DW ++YYEWLK AGKEVDLVE+P A+H
Sbjct: 252 HPAAHVFGPDAGDISGVKFPAXLLIVGGHDQLRDWGRKYYEWLKGAGKEVDLVEYPKAIH 311

Query: 303 GFYLVPGLEDDTSLFIKHASEFILKHMAV 332
           GFY VP L+ D SL IK A++F+ K +AV
Sbjct: 312 GFYAVPQLK-DASLLIKDANDFMQKQLAV 336

BLAST of Lsi08G001690 vs. NCBI nr
Match: XP_022974072.1 (probable carboxylesterase 18 [Cucurbita maxima])

HSP 1 Score: 478.0 bits (1229), Expect = 6.5e-131
Identity = 229/325 (70.46%), Postives = 266/325 (81.85%), Query Frame = 0

Query: 3   KVPLIFRAFLRVQSAALNVSLRPNRTVNRFIMNLFDPKIPPSSKPNDGVTTHDVVFDSTR 62
           K+PL F+  LR+ S A ++  RPN TVNR + NLFDPK  PSSKPN+GV+THD+VFD +R
Sbjct: 6   KLPLKFQILLRLHSFASSIGRRPNITVNRCLTNLFDPKFSPSSKPNNGVSTHDIVFDPSR 65

Query: 63  NLWFRVFVPCSDDSDNLTGLPLLVYFHGGGFSFLAANSMPFVDHCRRLARDLRVVVVSVN 122
           NLWFR+FVP S   D    LP++VYFHGGGF F +ANSMP+ D  RRLARDLRVVVVSVN
Sbjct: 66  NLWFRLFVPSSVSDD--VSLPVVVYFHGGGFVFFSANSMPYDDLGRRLARDLRVVVVSVN 125

Query: 123 YRLAPEHQYPIPYEDGFDALKFLDDLGSDNSDVFPVKVDFDRCFLAGDSAGGNLAHHVAV 182
           YRL+PEH+YP+PYEDGFD LKFLD++G D S VFP K D  RCFLAGDSAGGNLAHHVAV
Sbjct: 126 YRLSPEHRYPLPYEDGFDGLKFLDEMGFD-SVVFPAKADLGRCFLAGDSAGGNLAHHVAV 185

Query: 183 RAGGYHFKKLNIKGLIAIQPFFGGEERVESEVQYGKSAMLNLEQADWYWKAFLPHGSDRN 242
           RA GY FKK+NIKGLIAIQPFFGGEER  SEVQ+ KS ML+LEQADWYWKAFLP GS+RN
Sbjct: 186 RAAGYDFKKVNIKGLIAIQPFFGGEERTNSEVQFSKSPMLSLEQADWYWKAFLPDGSNRN 245

Query: 243 HPAAHVYGPDAEDISGVKFPATLLIVGGQDQLQDWQKRYYEWLKEAGKEVDLVEFPDAVH 302
           HPAAHV+G  A D+ G KFP TLLIVGG+DQL+DW  +YYEWLKE+ KEVDLVE+P+A+H
Sbjct: 246 HPAAHVFGAGAGDLPGEKFPTTLLIVGGRDQLRDWGMKYYEWLKESDKEVDLVEYPNAIH 305

Query: 303 GFYLVPGLEDDTSLFIKHASEFILK 328
           GFY VP L+ D SL IK  ++F+ K
Sbjct: 306 GFYAVPQLK-DCSLLIKDVNDFMQK 326

BLAST of Lsi08G001690 vs. NCBI nr
Match: XP_038889726.1 (probable carboxylesterase 18 [Benincasa hispida])

HSP 1 Score: 478.0 bits (1229), Expect = 6.5e-131
Identity = 230/329 (69.91%), Postives = 269/329 (81.76%), Query Frame = 0

Query: 3   KVPLIFRAFLRVQSAALNVSLRPNRTVNRFIMNLFDPKIPPSSKPNDGVTTHDVVFDSTR 62
           K+PL FR  LR+ S A  +S RPN TVNRF+M+ FDPKIPPSSKP  GVTT+DVVFD +R
Sbjct: 6   KLPLKFRILLRLHSFASFISRRPNITVNRFLMSFFDPKIPPSSKPRYGVTTNDVVFDPSR 65

Query: 63  NLWFRVFVPCSDDSDNLTGLPLLVYFHGGGFSFLAANSMPFVDHCRRLARDLRVVVVSVN 122
           NLWFR+F+P S    +   LP++VY+HGGGF F  ANSM + D CRRLAR+LRVVVVSVN
Sbjct: 66  NLWFRLFLPSSSVLADNISLPVVVYYHGGGFVFFWANSMAYDDLCRRLARELRVVVVSVN 125

Query: 123 YRLAPEHQYPIPYEDGFDALKFLDDLGSDNSDVFPVKVDFDRCFLAGDSAGGNLAHHVAV 182
           YRL+PEH+YPIPYEDGFDALKFLD +   N  VFPV VD  RCFLAGDSAGGNLAHHVAV
Sbjct: 126 YRLSPEHRYPIPYEDGFDALKFLDGVDL-NCGVFPVNVDVSRCFLAGDSAGGNLAHHVAV 185

Query: 183 RAGGYHFKKLNIKGLIAIQPFFGGEERVESEVQYGKSAMLNLEQADWYWKAFLPHGSDRN 242
           RAG + FKK+ IKGLIAIQP FGGEER +SE+Q+ +S ML L+Q DW WKAFLP+GS RN
Sbjct: 186 RAGSHDFKKVKIKGLIAIQPLFGGEERTDSEIQFKESPMLTLQQTDWSWKAFLPNGSSRN 245

Query: 243 HPAAHVYGPDAEDISGVKFPATLLIVGGQDQLQDWQKRYYEWLKEAGKEVDLVEFPDAVH 302
           HPAAHV+G    D+S + FPATLLIVGG+D LQDWQKRYYEWLKEAGKEVDLVE+P+A+H
Sbjct: 246 HPAAHVFGAGVGDMSSIVFPATLLIVGGRDLLQDWQKRYYEWLKEAGKEVDLVEYPNAIH 305

Query: 303 GFYLVPGLEDDTSLFIKHASEFILKHMAV 332
           GFY VP L+ D+SL IK A++FI KHM++
Sbjct: 306 GFYAVPELK-DSSLLIKDATDFIHKHMSL 332

BLAST of Lsi08G001690 vs. NCBI nr
Match: XP_008459512.1 (PREDICTED: probable carboxylesterase 18 [Cucumis melo])

HSP 1 Score: 474.9 bits (1221), Expect = 5.5e-130
Identity = 230/330 (69.70%), Postives = 271/330 (82.12%), Query Frame = 0

Query: 1   MEKVPLIFRAFLRVQSAALNVSLRPNRTVNRFIMNLFDPKIPPSSKPNDGVTTHDVVFDS 60
           MEK+PL FR  L   S A ++S R N TVNRF M+ FDPK  PSSKP +GV+T+DVVFD 
Sbjct: 1   MEKLPLKFRILLHFHSFASSISRRSNVTVNRFFMSFFDPKFSPSSKPRNGVSTYDVVFDP 60

Query: 61  TRNLWFRVFVPCSDDSD-NLTGLPLLVYFHGGGFSFLAANSMPFVDHCRRLARDLRVVVV 120
           + NLWFR+F+P S  +D N+T LP++VY+HGGGF F +ANSM + D CRRLAR+LRV VV
Sbjct: 61  SHNLWFRLFLPSSSSADKNVTDLPVVVYYHGGGFVFFSANSMAYDDFCRRLARELRVAVV 120

Query: 121 SVNYRLAPEHQYPIPYEDGFDALKFLDDLGSDNSDVFPVKVDFDRCFLAGDSAGGNLAHH 180
           SVNYRL+PEH+ PIPYEDGFDALKFLD +  D+   FPVK D  RCFLAGDSAGGNL HH
Sbjct: 121 SVNYRLSPEHRCPIPYEDGFDALKFLDGMDLDDGG-FPVKFDVSRCFLAGDSAGGNLTHH 180

Query: 181 VAVRAGGYHFKKLNIKGLIAIQPFFGGEERVESEVQYGKSAMLNLEQADWYWKAFLPHGS 240
           VAVRAGGY+FKKL IKG+IAIQPFFGGEERVESE+++ KS MLNL+Q+DWYWKAFLP G 
Sbjct: 181 VAVRAGGYNFKKLKIKGIIAIQPFFGGEERVESEIKFSKSPMLNLKQSDWYWKAFLPKGC 240

Query: 241 DRNHPAAHVYGPDA-EDISGVKFPATLLIVGGQDQLQDWQKRYYEWLK-EAGKEVDLVEF 300
           DRNHPAAHV+GP A +DIS VKFP TLLI+GG+DQL DW KRYYEWLK E GKEVDLVE+
Sbjct: 241 DRNHPAAHVFGPGAGDDISKVKFPTTLLILGGKDQLVDWGKRYYEWLKDECGKEVDLVEY 300

Query: 301 PDAVHGFYLVPGLEDDTSLFIKHASEFILK 328
           P+A+HGFY+VP L+ D+SL IK  ++FI K
Sbjct: 301 PNAIHGFYIVPELK-DSSLLIKDMNDFIHK 328

BLAST of Lsi08G001690 vs. TAIR 10
Match: AT5G23530.1 (carboxyesterase 18 )

HSP 1 Score: 304.3 bits (778), Expect = 1.2e-82
Identity = 148/320 (46.25%), Postives = 213/320 (66.56%), Query Frame = 0

Query: 4   VPLIFRAFLRVQSAALNVSLRPNRTVNRFIMNLFDPKIPPSSKPNDGVTTHDVVFDSTRN 63
           +PL  R  L V S   + + RP+ T+NR  + LFD + PP+ KP + V+T D V D +R+
Sbjct: 13  LPLKTRIALTVISTMTDNAQRPDGTINRRFLRLFDFRAPPNPKPVNIVSTSDFVVDQSRD 72

Query: 64  LWFRVFVP-CSDDSDNLTGLPLLVYFHGGGFSFLAANSMPFVDHCRRLARDLRVVVVSVN 123
           LWFR++ P  S D      +P++V+FHGGGF+FL+ N+ P+ + CRR AR L   V+SVN
Sbjct: 73  LWFRLYTPHVSGDK-----IPVVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPAYVISVN 132

Query: 124 YRLAPEHQYPIPYEDGFDALKFLDDLGSDNSDVFPVKVDFDRCFLAGDSAGGNLAHHVAV 183
           YRLAPEH+YP  Y+DGFDALK++++   ++  + P   D  RCF AGDSAGGN+AH+VA+
Sbjct: 133 YRLAPEHRYPAQYDDGFDALKYIEE---NHGSILPANADLSRCFFAGDSAGGNIAHNVAI 192

Query: 184 ---RAGGYHFKKLNIKGLIAIQPFFGGEERVESEVQYGKSAMLNLEQADWYWKAFLPHGS 243
              R     F  + + GLI+IQPFFGGEER E+E Q   + +++ ++ DW WKA    G 
Sbjct: 193 RICREPRSSFTAVKLIGLISIQPFFGGEERTEAEKQLVGAPLVSPDRTDWCWKAM---GL 252

Query: 244 DRNHPAAHVYGPDAEDISGVKFPATLLIVGGQDQLQDWQKRYYEWLKEAGKEVDLVEFPD 303
           +R+H A +V GP+A DISG+ +P T+++V G D L+DWQ+ YYEWLK  GK+  L+E+P+
Sbjct: 253 NRDHEAVNVGGPNAVDISGLDYPETMVVVAGFDPLKDWQRSYYEWLKLCGKKATLIEYPN 312

Query: 304 AVHGFYLVPGLEDDTSLFIK 320
             H FY+ P L +   L ++
Sbjct: 313 MFHAFYIFPELPEAGQLIMR 321

BLAST of Lsi08G001690 vs. TAIR 10
Match: AT5G27320.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 242.7 bits (618), Expect = 4.3e-64
Identity = 130/315 (41.27%), Postives = 185/315 (58.73%), Query Frame = 0

Query: 4   VPLIFRAFLRVQSAALNVSLRPNRTVNRFIMNLFDPKIPPSSKPNDGVTTHDVVFDSTRN 63
           VPL     +     A N+  RP+ T NR +    D K+P ++ P +GV + DV+ D   N
Sbjct: 16  VPLNTWVLISNFKLAYNLLRRPDGTFNRHLAEFLDRKVPANANPVNGVFSFDVIIDRQTN 75

Query: 64  LWFRVFVPC-SDDSDNLTGL---------PLLVYFHGGGFSFLAANSMPFVDHCRRLARD 123
           L  RV+ P  +  S ++T L         P++V+FHGG F+  +ANS  +   CRRL   
Sbjct: 76  LLSRVYRPADAGTSPSITDLQNPVDGEIVPVIVFFHGGSFAHSSANSAIYDTLCRRLVGL 135

Query: 124 LRVVVVSVNYRLAPEHQYPIPYEDGFDALKFLDDLGSDNSDVFPVKVDFDRCFLAGDSAG 183
              VVVSVNYR APE++YP  Y+DG+  LK++    + +S +   K    R FLAGDS+G
Sbjct: 136 CGAVVVSVNYRRAPENRYPCAYDDGWAVLKWV----NSSSWLRSKKDSKVRIFLAGDSSG 195

Query: 184 GNLAHHVAVRAGGYHFKKLNIKGLIAIQPFFGGEERVESEVQYGKSAMLNLEQADWYWKA 243
           GN+ H+VAVRA      ++++ G I + P FGG ER ESE +      + +   DWYW+A
Sbjct: 196 GNIVHNVAVRAVE---SRIDVLGNILLNPMFGGTERTESEKRLDGKYFVTVRDRDWYWRA 255

Query: 244 FLPHGSDRNHPAAHVYGPDAEDISGVKFPATLLIVGGQDQLQDWQKRYYEWLKEAGKEVD 303
           FLP G DR HPA   +GP ++ + G+ FP +L++V G D +QDWQ +Y E LK+AG+EV 
Sbjct: 256 FLPEGEDREHPACSPFGPRSKSLEGLSFPKSLVVVAGLDLIQDWQLKYAEGLKKAGQEVK 315

Query: 304 LVEFPDAVHGFYLVP 309
           L+    A  GFYL+P
Sbjct: 316 LLYLEQATIGFYLLP 323

BLAST of Lsi08G001690 vs. TAIR 10
Match: AT3G05120.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 236.9 bits (603), Expect = 2.4e-62
Identity = 130/333 (39.04%), Postives = 183/333 (54.95%), Query Frame = 0

Query: 4   VPLIFRAFLRVQSAALNVSLRPNRTVNRFIMNLFDPKIPPSSKPNDGVTTHDVVFDSTRN 63
           VPL     +     A N+  RP+ T NR +    D K+  ++ P DGV + DV+ D   N
Sbjct: 16  VPLNTWVLISNFKVAYNILRRPDGTFNRHLAEYLDRKVTANANPVDGVFSFDVLIDRRIN 75

Query: 64  LWFRVFVPCSDDSDNLTGL------------PLLVYFHGGGFSFLAANSMPFVDHCRRLA 123
           L  RV+ P   D +    +            P++++FHGG F+  +ANS  +   CRRL 
Sbjct: 76  LLSRVYRPAYADQEQPPSILDLEKPVDGDIVPVILFFHGGSFAHSSANSAIYDTLCRRLV 135

Query: 124 RDLRVVVVSVNYRLAPEHQYPIPYEDGFDALKFLDDLGSDNSDVFPVKVDFDRCFLAGDS 183
              + VVVSVNYR APE+ YP  Y+DG+ AL ++    +  S +   K      FLAGDS
Sbjct: 136 GLCKCVVVSVNYRRAPENPYPCAYDDGWIALNWV----NSRSWLKSKKDSKVHIFLAGDS 195

Query: 184 AGGNLAHHVAVRAGGYHFKKLNIKGLIAIQPFFGGEERVESEVQYGKSAMLNLEQADWYW 243
           +GGN+AH+VA+RAG      +++ G I + P FGG ER ESE        + +   DWYW
Sbjct: 196 SGGNIAHNVALRAGE---SGIDVLGNILLNPMFGGNERTESEKSLDGKYFVTVRDRDWYW 255

Query: 244 KAFLPHGSDRNHPAAHVYGPDAEDISGVKFPATLLIVGGQDQLQDWQKRYYEWLKEAGKE 303
           KAFLP G DR HPA + + P  + + GV FP +L++V G D ++DWQ  Y E LK+AG+E
Sbjct: 256 KAFLPEGEDREHPACNPFSPRGKSLEGVSFPKSLVVVAGLDLIRDWQLAYAEGLKKAGQE 315

Query: 304 VDLVEFPDAVHGFYLVP------GLEDDTSLFI 319
           V L+    A  GFYL+P       + D+ S F+
Sbjct: 316 VKLMHLEKATVGFYLLPNNNHFHNVMDEISAFV 341

BLAST of Lsi08G001690 vs. TAIR 10
Match: AT3G63010.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 231.9 bits (590), Expect = 7.6e-61
Identity = 130/339 (38.35%), Postives = 188/339 (55.46%), Query Frame = 0

Query: 4   VPLIFRAFLRVQSAALNVSLRPNRTVNRFIMNLFDPKIPPSSKPNDGVTTHDVVFDSTRN 63
           VPL     +     A  V  RP+ + NR +    D K+P +S P DGV + D V DST N
Sbjct: 16  VPLNTWVLISNFKLAYKVLRRPDGSFNRDLAEFLDRKVPANSFPLDGVFSFDHV-DSTTN 75

Query: 64  LWFRVFVPCSDDSDNLTG-------------LPLLVYFHGGGFSFLAANSMPFVDHCRRL 123
           L  R++ P S       G             +P+L++FHGG F+  +ANS  +   CRRL
Sbjct: 76  LLTRIYQPASLLHQTRHGTLELTKPLSTTEIVPVLIFFHGGSFTHSSANSAIYDTFCRRL 135

Query: 124 ARDLRVVVVSVNYRLAPEHQYPIPYEDGFDALKFLDD----LGSDNSDVFPVKVDFDRCF 183
                VVVVSV+YR +PEH+YP  Y+DG++AL ++          +S+V+         +
Sbjct: 136 VTICGVVVVSVDYRRSPEHRYPCAYDDGWNALNWVKSRVWLQSGKDSNVY--------VY 195

Query: 184 LAGDSAGGNLAHHVAVRAGGYHFKKLNIKGLIAIQPFFGGEERVESEVQYGKSAMLNLEQ 243
           LAGDS+GGN+AH+VAVRA     K L   G I + P FGG+ER +SE        + ++ 
Sbjct: 196 LAGDSSGGNIAHNVAVRATNEGVKVL---GNILLHPMFGGQERTQSEKTLDGKYFVTIQD 255

Query: 244 ADWYWKAFLPHGSDRNHPAAHVYGPDAEDISGVKFPATLLIVGGQDQLQDWQKRYYEWLK 303
            DWYW+A+LP G DR+HPA + +GP  + + GV FP +L++V G D +QDWQ  Y + LK
Sbjct: 256 RDWYWRAYLPEGEDRDHPACNPFGPRGQSLKGVNFPKSLVVVAGLDLVQDWQLAYVDGLK 315

Query: 304 EAGKEVDLVEFPDAVHGFYLVPGLEDDTSLFIKHASEFI 326
           + G EV+L+    A  GFY +P   D     ++  ++F+
Sbjct: 316 KTGLEVNLLYLKQATIGFYFLPN-NDHFHCLMEELNKFV 341

BLAST of Lsi08G001690 vs. TAIR 10
Match: AT5G06570.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 195.7 bits (496), Expect = 6.0e-50
Identity = 115/293 (39.25%), Postives = 166/293 (56.66%), Query Frame = 0

Query: 21  VSLRPNRTVNRF-IMNLFDPKIPPSSKPNDGVTTHDVVFDSTRNLWFRVFVPCSDDSDNL 80
           + L  N TV R   ++L   +IP   K N  V   D ++    NL  R++ P S  + N 
Sbjct: 18  LQLLSNGTVLRSESIDLITQQIP--FKNNQTVLFKDSIYHKPNNLHLRLYKPIS--ASNR 77

Query: 81  TGLPLLVYFHGGGFSFLAANSMPFVDHCRRLARDLRVVVVSVNYRLAPEHQYPIPYEDGF 140
           T LP++V+FHGGGF F + +   F + C  LA  L  +VVS +YRLAPEH+ P  +ED  
Sbjct: 78  TALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPAAFEDAE 137

Query: 141 DALKFL-DDLGSDNSDVF---PVKVDFDRCFLAGDSAGGNLAHHVAVR--AGGYHFKKLN 200
             L +L D   SD  + +      VDFDR F+ GDS+GGN+AH +AVR  +G      + 
Sbjct: 138 AVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFGSGSIELTPVR 197

Query: 201 IKGLIAIQPFFGGEERVESEVQYGKSAMLNLEQADWYWKAFLPHGSDRNHPAAHVYGPDA 260
           ++G + + PFFGGEER  SE      A+L+L+  D +W+  LP+G+ R+H  A+ +GP +
Sbjct: 198 VRGYVLMGPFFGGEERTNSE-NGPSEALLSLDLLDKFWRLSLPNGATRDHHMANPFGPTS 257

Query: 261 EDISGVKFPATLLIVGGQDQLQDWQKRY-YEWLKEAGKEVDLVEFPDAVHGFY 306
             +  +     L+IVGG + L+D  K Y Y+  K  GK VD +EF +  HGFY
Sbjct: 258 PTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKEHGFY 305

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LT101.7e-8146.25Probable carboxylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=CXE18 PE=1 SV=1[more]
Q940G66.0e-6341.27Gibberellin receptor GID1C OS=Arabidopsis thaliana OX=3702 GN=GID1C PE=1 SV=1[more]
Q9MAA73.3e-6139.04Gibberellin receptor GID1A OS=Arabidopsis thaliana OX=3702 GN=GID1A PE=1 SV=1[more]
Q9LYC11.1e-5938.35Gibberellin receptor GID1B OS=Arabidopsis thaliana OX=3702 GN=GID1B PE=1 SV=1[more]
Q6L5459.0e-5134.65Gibberellin receptor GID1 OS=Oryza sativa subsp. japonica OX=39947 GN=GID1 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1DXC51.4e-13169.91probable carboxylesterase 18 OS=Momordica charantia OX=3673 GN=LOC111023986 PE=4... [more]
A0A6J1I9943.2e-13170.46probable carboxylesterase 18 OS=Cucurbita maxima OX=3661 GN=LOC111472693 PE=4 SV... [more]
A0A1S3CAV22.7e-13069.70probable carboxylesterase 18 OS=Cucumis melo OX=3656 GN=LOC103498628 PE=4 SV=1[more]
A0A6J1EW522.1e-12768.09probable carboxylesterase 18 OS=Cucurbita moschata OX=3662 GN=LOC111436652 PE=4 ... [more]
A0A0A0KUP02.3e-12667.77Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G6473... [more]
Match NameE-valueIdentityDescription
XP_038890672.11.4e-13672.21probable carboxylesterase 18 [Benincasa hispida][more]
XP_022157226.12.9e-13169.91probable carboxylesterase 18 [Momordica charantia][more]
XP_022974072.16.5e-13170.46probable carboxylesterase 18 [Cucurbita maxima][more]
XP_038889726.16.5e-13169.91probable carboxylesterase 18 [Benincasa hispida][more]
XP_008459512.15.5e-13069.70PREDICTED: probable carboxylesterase 18 [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT5G23530.11.2e-8246.25carboxyesterase 18 [more]
AT5G27320.14.3e-6441.27alpha/beta-Hydrolases superfamily protein [more]
AT3G05120.12.4e-6239.04alpha/beta-Hydrolases superfamily protein [more]
AT3G63010.17.6e-6138.35alpha/beta-Hydrolases superfamily protein [more]
AT5G06570.16.0e-5039.25alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 1..326
e-value: 1.7E-91
score: 309.0
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 26..327
IPR013094Alpha/beta hydrolase fold-3PFAMPF07859Abhydrolase_3coord: 85..305
e-value: 4.0E-57
score: 193.5
NoneNo IPR availablePANTHERPTHR23024:SF434ACETYL ESTERASEcoord: 3..326
NoneNo IPR availablePANTHERPTHR23024ARYLACETAMIDE DEACETYLASEcoord: 3..326

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi08G001690.1Lsi08G001690.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity