Lsi08G001330 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi08G001330 vs. ExPASy Swiss-Prot
Match: A0A290U7C4 (Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV=1)

HSP 1 Score: 379.8 bits (974), Expect = 7.1e-104
Identity = 248/695 (35.68%), Postives = 374/695 (53.81%), Query Frame = 0

Query: 2   NTRNTFTGYLYKELVELGISTFMDDKKLLIGDRLSKKLIRAIEKSGSFIVVLSKNYASSK 61
           +TR TF G+L+  L+E GI TFMDDK+L  G  +S +L++AI +S   +VV SKNYASS 
Sbjct: 22  DTRKTFVGHLFNALIEKGIHTFMDDKELKRGKSISSELMKAIGESRFAVVVFSKNYASST 81

Query: 62  WCLRELTKIIDCMAEQKHRVLPVFYHVSPRDVRRQSGCFEKSFHEHEKILLELDNMERDK 121
           WCL EL KI++   + +  V+PVFY V P  VR+Q+G +   F + E  L++    +RDK
Sbjct: 82  WCLEELVKILEIHEKFELIVVPVFYDVDPSTVRKQNGEYAVCFTKFEANLVD----DRDK 141

Query: 122 YMKEVQQWRRALTKVGDLSG----EVANIVEVVSIGKITKQLFDMLHHQKLVASDKLNQL 181
               V +WR ALTKV ++SG       N  E   I +I K +FD       + +     L
Sbjct: 142 ----VLRWREALTKVANISGHDLRNTYNGDESKCIQQILKDIFDKFCFSISITN---RDL 201

Query: 182 VDIERRLCKMDMLIDLKSNKVHFVGIIGMGGIGKTTIAEVFYDKVAYKFGKNRCFLRTYE 241
           V IE ++ K+  L+ +    V  VGI GMGG+GKTT A   +++    F ++ CFL   +
Sbjct: 202 VGIESQIKKLSSLLRMDLKGVRLVGIWGMGGVGKTTAARALFNRYYQNF-ESACFLEDVK 261

Query: 242 -----HTLVSLQQQLLSQLLQTKDIIINNENEGATMIGSYLKEKKVLIVLDGVKDKSQLE 301
                HTL+ LQ+ LLS+LL+ + +   +  E   ++   L  KKVL+VLD V    QL+
Sbjct: 262 EYLQHHTLLYLQKTLLSKLLKVEFVDCTDTEEMCVILKRRLCSKKVLVVLDDVNHNDQLD 321

Query: 302 KLVGSPNWFGQGSQIIITTRNRDVLRQPNYKDKMVEYNVEFLDYKSAMSLFCKHAFGCEF 361
           KLVG+ +WFG GS+I+ITTR+  +L+     D    Y ++ L+   A+ LF  HAF    
Sbjct: 322 KLVGAEDWFGSGSRIVITTRDMKLLKN---HDVHETYEIKVLEKDEAIELFNLHAFKRSS 381

Query: 362 PNKNYEDFCKEIVERVKGHPQALIQIGLSLYDQDIDIWKEELKNLREDYNKHLFKTLKIS 421
           P K +++    +V+   G P AL  +G  LY +D+D+W   +  L+++    +  TLKIS
Sbjct: 382 PEKEFKELLNLVVDYTGGLPLALKVLGSLLYKEDLDVWISTIDRLKDNPEGEIMATLKIS 441

Query: 422 FDDLGKTSQEAFLDLACFFNGKKKEKVIEILKSFDYRPHSEIQLLQDRCLIEVRRDNSIF 481
           FD L    +  FLD+ACFF G  +  +  +  +  + P   ++ L ++ LI +  D  I 
Sbjct: 442 FDGLRDYEKSIFLDIACFFRGYNQRDMTALFHASGFHPVLGVKTLVEKSLIFILED-KIQ 501

Query: 482 MPKCIQAMGQEIEREADKRSRIWLPKDALDVFDEGHRVKEIKGIVLEGEEKQDEVELE-- 541
           M   +Q MG++I  +     RI+ P+D  D      R + I+G++L   E+ +E ELE  
Sbjct: 502 MHDLMQEMGRQIAVQESPMRRIYRPEDVKDACIGDMRKEAIEGLLLTEPEQFEEGELEYM 561

Query: 542 --GRAFKEMTCLKIL--EMSNVQLRGDFTHLSKQLRLLNWHNYPSQCLPLRFESRYLFEL 601
               A K+   L+IL  E  N        +L   L  L W NY S   P  FE   L  L
Sbjct: 562 YSAEALKKTRRLRILVKEYYNRGFDEPVAYLPNSLLWLEWRNYSSNSFPSNFEPSKLVYL 621

Query: 602 LLPLSQTRQLWNGQK------VINISDSKNLRETPNFTKVPNLESLVLSNCTRLWKIDSS 661
            +  S   +LWNG K       +++S    L +TP+F  + NLE L+LS+C  L ++  S
Sbjct: 622 TMKGSSIIELWNGAKRLAFLTTLDLSYCHKLIQTPDFRMITNLERLILSSCDALVEVHPS 681

Query: 662 ISRLNRLTLLDLTCCINLKSIPSIKSCKSLTTLNL 676
           +  L  L LL++  CI+L+ +P+I   + L  L+L
Sbjct: 682 VGFLKNLILLNMDHCISLERLPAIIQSECLEVLDL 700

BLAST of Lsi08G001330 vs. ExPASy Swiss-Prot
Match: V9M2S5 (Disease resistance protein RPV1 OS=Vitis rotundifolia OX=103349 GN=RPV1 PE=1 SV=1)

HSP 1 Score: 370.5 bits (950), Expect = 4.3e-101
Identity = 274/777 (35.26%), Postives = 395/777 (50.84%), Query Frame = 0

Query: 2   NTRNTFTGYLYKELVELGISTFMDDKKLLIGDRLSKKLIRAIEKSGSFIVVLSKNYASSK 61
           +TR  FT +LY  L   GI TF DD +L  G+ ++ +L++AIE+S S ++V S+NYA S+
Sbjct: 34  DTRYNFTDHLYSALGRRGIRTFRDD-RLRRGEAIAPELLKAIEESRSSVIVFSENYAHSR 93

Query: 62  WCLRELTKIIDCMAEQKHRVLPVFYHVSPRDVRRQSGCFEKSFHEHEKILLELDNMERDK 121
           WCL EL KI++C  +  H V P+FYHV P  VR+Q G F ++F  +E           + 
Sbjct: 94  WCLDELVKIMECQKDLGHAVFPIFYHVDPSHVRKQEGSFGEAFAGYE-----------EN 153

Query: 122 YMKEVQQWRRALTKVGDLSG--EVANIVEVVSIGKITKQLFDMLHHQKLVASDKLNQLVD 181
           +  ++ +WR ALT+  +LSG   + +  E   I +IT  +F  L  ++L   D    LV 
Sbjct: 154 WKDKIPRWRTALTEAANLSGWHLLDDRYESNQIKEITNSIFRQLKCKRL---DVGANLVG 213

Query: 182 IERRLCKMDMLIDLKSNKVHFVGIIGMGGIGKTTIAEVFYDKVAYKFGKNRCFLRTYE-- 241
           I+  + +M + + L+S+ V  VGI G+GGIGKTTIA+V Y++++ +F +   FL      
Sbjct: 214 IDSHVKEMILRLHLESSDVRMVGIYGVGGIGKTTIAKVIYNELSCEF-EYMSFLENIREG 273

Query: 242 ---HTLVSLQQQLLSQLLQTK-DIIINNENEGATMIGSYLKEKKVLIVLDGVKDKSQLEK 301
                L  LQ QLL  +L+ +    I++    A+MI   L  ++V IVLD V D SQLE 
Sbjct: 274 SNPQVLFHLQNQLLGDILEGEGSQNISSVAHRASMIKDILLSRRVFIVLDDVDDLSQLEY 333

Query: 302 LVGSPNWFGQGSQIIITTRNRDVLRQPNYKDKMVEYNVEFLDYKSAMSLFCKHAFGCEFP 361
           L+G   W G+GS++IITTRN+ VL      D    Y VE L+++ A  LF  +AF    P
Sbjct: 334 LLGHREWLGEGSRVIITTRNKHVLAVQEVDDL---YEVEGLNFEEACELFSLYAFKQNLP 393

Query: 362 NKNYEDFCKEIVERVKGHPQALIQIGLSLYDQDIDIWKEELKNLREDYNKHLFKTLKISF 421
             +Y +    +V   +G P AL  +G  L  + I  W+ ELK L  +    + K LK S+
Sbjct: 394 KSDYRNLTCRVVGYCQGLPLALKVLGSLLCKKTIPQWEGELKKLDSEPKADIHKVLKRSY 453

Query: 422 DDLGKTSQEAFLDLACFFNGKKKEKVIEILKSFDYRPHSEIQLLQDRCLIEVRRDNSIFM 481
           D L +  +  FLDLACFF G+ ++ V+ IL   D+   + I  L D CLI +   N I M
Sbjct: 454 DGLDRIDKNIFLDLACFFKGEGRDFVLRILDGCDFPAETGISNLNDLCLITLPY-NQICM 513

Query: 482 PKCIQAMGQEIER-----EADKRSRIWLPKDALDVFDEGHRVKEIKGIVLEGEEKQDEVE 541
              IQ MG EI R     E +K SR+W P D          +K ++ + L+   K   V 
Sbjct: 514 HDLIQQMGWEIVRENFPVEPNKWSRLWDPCDFERALTADEGIKSVETMSLD-LSKLKRVC 573

Query: 542 LEGRAFKEMTCLKILEM----------------------------SNVQLRGDFTHLSKQ 601
                F +MT L++L++                            S +QL   F   S +
Sbjct: 574 SNSNVFAKMTKLRLLKVYSSSDIDSAHGDSDEDIEEVYDVVMKDASKMQLGQSFKFPSYE 633

Query: 602 LRLLNWHNYPSQCLPLRFESRYLFELLLPLSQTRQLWNGQ------KVINISDSKNLRET 661
           LR L W  YP   LPL F+   L EL L  S  +QLW G       KVI++S S+ L + 
Sbjct: 634 LRYLRWDGYPLDSLPLNFDGGKLVELHLKCSNIKQLWQGHKDLERLKVIDLSYSRKLSQM 693

Query: 662 PNFTKVPNLESLVLSNCTRLWKIDSSISRLNRLTLLDLTCCINLKSIP-SIKSCKSLTTL 721
             F+ +PNLE L LS C  L  I  S+  + +LT L L  C  LK++P SI   +SL +L
Sbjct: 694 SEFSSMPNLERLCLSGCVSLIDIHPSVGNMKKLTTLSLRSCNKLKNLPDSIGDLESLESL 753

Query: 722 NLAGSGLIGL--EHSQNVFQLAK--RRSTIISSPIDQDLYSGSTLSAFEDLYSSNNS 727
            L+         E   N+  L +   ++T I    D    S   L + E LY SN S
Sbjct: 754 YLSNCSKFEKFPEKGGNMKSLTELDLKNTAIKDLPD----SIGDLESLESLYLSNCS 785

BLAST of Lsi08G001330 vs. ExPASy Swiss-Prot
Match: Q40392 (TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1)

HSP 1 Score: 366.7 bits (940), Expect = 6.2e-100
Identity = 245/706 (34.70%), Postives = 369/706 (52.27%), Query Frame = 0

Query: 2   NTRNTFTGYLYKELVELGISTFMDDKKLLIGDRLSKKLIRAIEKSGSFIVVLSKNYASSK 61
           +TR TFT +LY+ L + GI TF DDK+L  G  +  +L +AIE+S   IVV S+NYA+S+
Sbjct: 22  DTRKTFTSHLYEVLNDKGIKTFQDDKRLEYGATIPGELCKAIEESQFAIVVFSENYATSR 81

Query: 62  WCLRELTKIIDCMAEQKHRVLPVFYHVSPRDVRRQSGCFEKSFHEHEKILLELDNMERDK 121
           WCL EL KI++C    K  V+P+FY V P  VR Q   F K+F EHE            K
Sbjct: 82  WCLNELVKIMECKTRFKQTVIPIFYDVDPSHVRNQKESFAKAFEEHE-----------TK 141

Query: 122 YMKEV---QQWRRALTKVGDLSGEVAN--IVEVVSIGKITKQLFDMLHHQKLVASDKLNQ 181
           Y  +V   Q+WR AL +  +L G   N    +   I +I  Q+   L     ++   L  
Sbjct: 142 YKDDVEGIQRWRIALNEAANLKGSCDNRDKTDADCIRQIVDQISSKLCK---ISLSYLQN 201

Query: 182 LVDIERRLCKMDMLIDLKSNKVHFVGIIGMGGIGKTTIAEVFYDKV------AYKFGKNR 241
           +V I+  L K++ L+++  N V  +GI GMGG+GKTTIA   +D +      +Y+F    
Sbjct: 202 IVGIDTHLEKIESLLEIGINGVRIMGIWGMGGVGKTTIARAIFDTLLGRMDSSYQF-DGA 261

Query: 242 CFLRTYEHT---LVSLQQQLLSQLLQTKDIIINNENEGATMIGSYLKEKKVLIVLDGVKD 301
           CFL+  +     + SLQ  LLS+LL+ K    NNE +G   + S L+ KKVLIVLD + +
Sbjct: 262 CFLKDIKENKRGMHSLQNALLSELLREK-ANYNNEEDGKHQMASRLRSKKVLIVLDDIDN 321

Query: 302 KSQ-LEKLVGSPNWFGQGSQIIITTRNRDVLRQPNYKDKMVEYNVEFLDYKSAMSLFCKH 361
           K   LE L G  +WFG GS+IIITTR++ ++ + +     + Y V  L    ++ LF +H
Sbjct: 322 KDHYLEYLAGDLDWFGNGSRIIITTRDKHLIEKND-----IIYEVTALPDHESIQLFKQH 381

Query: 362 AFGCEFPNKNYEDFCKEIVERVKGHPQALIQIGLSLYDQDIDIWKEELKNLREDYNKHLF 421
           AFG E PN+N+E    E+V   KG P AL   G  L++  +  WK  +++++ +    + 
Sbjct: 382 AFGKEVPNENFEKLSLEVVNYAKGLPLALKVWGSLLHNLRLTEWKSAIEHMKNNSYSGII 441

Query: 422 KTLKISFDDLGKTSQEAFLDLACFFNGKKKEKVIEILKSFDYRPHSEIQLLQDRCLIEVR 481
             LKIS+D L    QE FLD+ACF  G++K+ +++IL+S        +++L D+ L+ + 
Sbjct: 442 DKLKISYDGLEPKQQEMFLDIACFLRGEEKDYILQILESCHIGAEYGLRILIDKSLVFIS 501

Query: 482 RDNSIFMPKCIQAMGQEI---EREADKRSRIWLPKDALDVFDEGHRVKEIKGIVLEGEEK 541
             N + M   IQ MG+ I   +++  +RSR+WL K+  +V         ++ I +     
Sbjct: 502 EYNQVQMHDLIQDMGKYIVNFQKDPGERSRLWLAKEVEEVMSNNTGTMAMEAIWV--SSY 561

Query: 542 QDEVELEGRAFKEMTCLKILEMSNVQLRGDFTHLSKQLRLLNWHNYPSQCLPLRFESRYL 601
              +    +A K M  L++  M          +L   LR     NYP +  P  FE + L
Sbjct: 562 SSTLRFSNQAVKNMKRLRVFNMGRSSTHYAIDYLPNNLRCFVCTNYPWESFPSTFELKML 621

Query: 602 FELLLPLSQTRQLWNGQK------VINISDSKNLRETPNFTKVPNLESLVLSNCTRLWKI 661
             L L  +  R LW   K       I++S SK L  TP+FT +PNLE + L  C+ L ++
Sbjct: 622 VHLQLRHNSLRHLWTETKHLPSLRRIDLSWSKRLTRTPDFTGMPNLEYVNLYQCSNLEEV 681

Query: 662 DSSISRLNRLTLLDLTCCINLKSIP----------SIKSCKSLTTL 674
             S+   +++  L L  C +LK  P           ++SC SL  L
Sbjct: 682 HHSLGCCSKVIGLYLNDCKSLKRFPCVNVESLEYLGLRSCDSLEKL 704

BLAST of Lsi08G001330 vs. ExPASy Swiss-Prot
Match: V9M398 (Disease resistance protein RUN1 OS=Vitis rotundifolia OX=103349 GN=RUN1 PE=1 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 8.4e-97
Identity = 259/726 (35.67%), Postives = 379/726 (52.20%), Query Frame = 0

Query: 2   NTRNTFTGYLYKELVELGISTFMDDKKLLIGDRLSKKLIRAIEKSGSFIVVLSKNYASSK 61
           +TR  FT +LY  L   GI TF DD KL  G+ ++ +L++AIE+S S ++V S+NYA S+
Sbjct: 37  DTRFNFTDHLYSALGRRGIRTFRDD-KLRRGEAIAPELLKAIEESRSSVIVFSENYARSR 96

Query: 62  WCLRELTKIIDCMAEQK---HRVLPVFYHVSPRDVRRQSGCFEKSFHEHEKILLELDNME 121
           WCL EL KI++C  ++K   H V P+FYHV P  VR+Q G F ++F  + + L       
Sbjct: 97  WCLDELVKIMECHKDKKDPGHAVFPIFYHVDPSHVRKQEGSFGEAFAGYGENL------- 156

Query: 122 RDKYMKEVQQWRRALTKVGDLSG-EVANIVEVVSIGKITKQLFDMLHHQKLVASDKLNQL 181
           +DK    + +WR ALT+  +LSG  + +  E   I +IT  +F  L  ++L   D    L
Sbjct: 157 KDK----IPRWRTALTEAANLSGWPLQDGYESNQIKEITDSIFRRLKCKRL---DAGANL 216

Query: 182 VDIERRLCKMDMLIDLKSNKVHFVGIIGMGGIGKTTIAEVFYDKVAYKFGKNRCFLRTYE 241
           V I+  + +M   + ++S+ V  VG+ G+GGIGKTTIA+V Y++++ +F +   FL    
Sbjct: 217 VGIDSHVKEMIWRLHMESSDVRMVGMYGVGGIGKTTIAKVIYNELSREF-EYMSFLENIR 276

Query: 242 HT-----LVSLQQQLLSQLLQTK-DIIINNENEGATMIGSYLKEKKVLIVLDGVKDKSQL 301
                  +  LQ QLL  +L+ +    IN+   GA+MI   L  K V IVLD V D+SQL
Sbjct: 277 EKFNTQGVSPLQNQLLDDILKGEGSQNINSVAHGASMIKDILSSKIVFIVLDDVDDQSQL 336

Query: 302 EKLVGSPNWFGQGSQIIITTRNRDVLRQPNYKDKMVEYNVEFLDYKSAMSLFCKHAFGCE 361
           E L+    W G+GS++IITTRN+ VL      D    Y V+ L+++ A  LF  +AF   
Sbjct: 337 EYLLRHREWLGEGSRVIITTRNKHVLDVQKVDDL---YEVKGLNFEEACELFSLYAFEQN 396

Query: 362 FPNKNYEDFCKEIVERVKGHPQALIQIGLSLYDQDIDIWKEELKNLREDYNKHLFKTLKI 421
            P  +Y +    +V   +G P AL  +G  L  + I  W+ EL+ L  +    +   LK 
Sbjct: 397 LPKSDYRNLSHRVVGYCQGLPLALKVLGCLLLKKTIPEWESELRKLDREPEAEILSVLKR 456

Query: 422 SFDDLGKTSQEAFLDLACFFNGKKKEKVIEILKSFDYRPHSEIQLLQDRCLIEVRRDNSI 481
           S+D LG+T +  FLD+ACFF G+ ++ V +IL + D+     I+ L D+CLI ++  N I
Sbjct: 457 SYDGLGRTEKSIFLDVACFFKGEDRDFVSKILDACDFHAEIGIKNLNDKCLITLQY-NRI 516

Query: 482 FMPKCIQAMGQEIER-----EADKRSRIWLPKDALDVFDEGHRVKEIKGIVLEGEEKQDE 541
            M   IQ MG EI R     E +K SR+W   D          +K ++ I L+   K   
Sbjct: 517 RMHDLIQQMGWEIVREKFPDEPNKWSRLWDTCDFERALTAYKGIKRVETISLD-LSKLKR 576

Query: 542 VELEGRAFKEMTCLKILEM-----------------------------SNVQLRGDFTHL 601
           V     AF +MT L++L++                             S ++L   F   
Sbjct: 577 VCSNSNAFAKMTRLRLLKVQSSLDIDFEPEYIDADDKVELYDVVMKNASKMRLGRGFKFP 636

Query: 602 SKQLRLLNWHNYPSQCLPLRFESRYLFELLLPLSQTRQLWNGQ------KVINISDSKNL 661
           S +LR L W  YP   LP  F+   L EL L  S  +QL  G       KVI++S S+ L
Sbjct: 637 SYELRYLRWDGYPLDFLPSNFDGGKLVELHLKCSNIKQLRLGNKDLEMLKVIDLSYSRKL 696

Query: 662 RETPNFTKVPNLESLVLSNCTRLWKIDSSISRLNRLTLLDLTCCINLKSIP-SIKSCKSL 677
            +   F+ +PNLE L L  C  L  I  S+  + +LT L L  C  LK++P SI   +SL
Sbjct: 697 SQMSEFSSMPNLERLFLRGCVSLIDIHPSVGNMKKLTTLSLKSCKKLKNLPDSIGDLESL 741

BLAST of Lsi08G001330 vs. ExPASy Swiss-Prot
Match: Q9SZ66 (Disease resistance-like protein DSC1 OS=Arabidopsis thaliana OX=3702 GN=DSC1 PE=1 SV=1)

HSP 1 Score: 336.3 bits (861), Expect = 9.0e-91
Identity = 233/718 (32.45%), Postives = 358/718 (49.86%), Query Frame = 0

Query: 2   NTRNTFTGYLYKELVELGISTFMDDKKLLIGDRLSKKLIRAIEKSGSFIVVLSKNYASSK 61
           +TRN FTG+L K L   GI +F+DD +L  GD L+  L   IEKS   I+V S NYA+S 
Sbjct: 21  DTRNNFTGHLQKALRLRGIDSFIDD-RLRRGDNLT-ALFDRIEKSKIAIIVFSTNYANSA 80

Query: 62  WCLRELTKIIDCMAEQKHRVLPVFYHVSPRDVRRQSGCFEKSFHEHEKILLELDNMERDK 121
           WCLREL KI++C    +  V+P+FY V   DV +Q   F   F   E     +       
Sbjct: 81  WCLRELVKILECRNSNQQLVVPIFYKVDKSDVEKQRNSFAVPFKLPELTFPGVT------ 140

Query: 122 YMKEVQQWRRALTKVGDLSGEVANIVEVVSIGKITKQLFDMLHHQKLVASDKLNQLVDIE 181
             +E+  W+ AL    ++ G V   +       + +   D       +A      LV IE
Sbjct: 141 -PEEISSWKAALASASNILGYVVKEISTSEAKLVDEIAVDTFKKLNDLAPSGNEGLVGIE 200

Query: 182 RRLCKMDMLIDLKS-NKVHFVGIIGMGGIGKTTIAEVFYDKVAYKFGKNRCFLRTYEHT- 241
            RL  ++ L+  +  + VH +GI+GM GIGKTT+A+  Y ++  +F    CFL       
Sbjct: 201 SRLKNLEKLLSWEDLDTVHIIGIVGMVGIGKTTLADCLYGRMRGQF-DGSCFLTNIRENS 260

Query: 242 ----LVSLQQQLLSQLLQTKDIIINNENEGATMIGSYLKEKKVLIVLDGVKDKSQLEKLV 301
               L SL Q+L S +L  +D+ I             LK K++LIVLD V D+ Q+  L+
Sbjct: 261 GRSGLESLLQKLFSTVLNDRDLEIGAPGNAHERFERRLKSKRLLIVLDDVNDEKQIRYLM 320

Query: 302 GSPNWFGQGSQIIITTRNRDVLRQPNYKDKMVEYNVEFLDYKSAMSLFCKHAFGCEFPNK 361
           G   W+  GS+IIITTR+  ++       K  +Y +  L+ + A+ LF  +AF   FP K
Sbjct: 321 GHCKWYQGGSRIIITTRDSKLIE----TIKGRKYVLPKLNDREALKLFSLNAFSNSFPLK 380

Query: 362 NYEDFCKEIVERVKGHPQALIQIGLSLYDQDIDIWKEELKNLREDYNKHLFKTLKISFDD 421
            +E     +++  KGHP AL  +G  L ++D   W+ +L  L+   +  +++ L+ S+++
Sbjct: 381 EFEGLTNMVLDYAKGHPLALKVLGSDLCERDDLYWEAKLDRLKSRSHGDIYEVLETSYEE 440

Query: 422 LGKTSQEAFLDLACFFNGKKKEKVIEILKSFDYRPHSEIQLLQDRCLIEVRRDNSIFMPK 481
           L    +  FLD+ACFF  +  + V  +L S        ++ L D+CLI +  DN I M  
Sbjct: 441 LTTEQKNVFLDIACFFRSENVDYVTSLLNSHGVDVSGVVKDLVDKCLITL-SDNRIEMHD 500

Query: 482 CIQAMGQEIEREADK------------------RSRIWLPKDALDVFDEGHRVKEIKGIV 541
            +Q M +EI  + +                     R+W  +D  D+  EG    +I+GI 
Sbjct: 501 MLQTMAKEISLKVETIGIRDCRWLSRHGNQCQWHIRLWDSEDICDLLTEGLGTDKIRGIF 560

Query: 542 LEGEEKQDEVELEGRAFKEMTCLKILEMSN------------VQLRGDFTHLSKQLRLLN 601
           L+   K   + L  +AF+ M  LK L++ +            + LR   + L  +L  L+
Sbjct: 561 LD-TSKLRAMRLSAKAFQGMYNLKYLKIYDSHCSRGCEAEFKLHLRRGLSFLPNELTYLH 620

Query: 602 WHNYPSQCLPLRFESRYLFELLLPLSQTRQLWNGQ------KVINISDSKNLRETPNFTK 661
           WH YP Q +PL F+ + L +L LP SQ  ++W+ +      K +++S S NLR+      
Sbjct: 621 WHGYPLQSIPLDFDPKNLVDLKLPHSQLEEIWDDEKDVGMLKWVDLSHSINLRQCLGLAN 680

Query: 662 VPNLESLVLSNCTRLWKIDSSISRLNRLTLLDLTCCINLKSIPSIKSCKSLTTLNLAG 678
             NLE L L  CT L K+ S+I+ L +L  L+L  C +L+S+P     +SL TL L+G
Sbjct: 681 AHNLERLNLEGCTSLKKLPSTINCLEKLIYLNLRDCTSLRSLPKGIKTQSLQTLILSG 722

BLAST of Lsi08G001330 vs. ExPASy TrEMBL
Match: A0A1S3C9Z0 (TMV resistance protein N-like OS=Cucumis melo OX=3656 GN=LOC103498649 PE=4 SV=1)

HSP 1 Score: 1101.3 bits (2847), Expect = 0.0e+00
Identity = 565/713 (79.24%), Postives = 627/713 (87.94%), Query Frame = 0

Query: 2   NTRNTFTGYLYKELVELGISTFMDDKKLLIGDRLSKKLIRAIEKSGSFIVVLSKNYASSK 61
           +TRNTFTG+LYKELV LGI+TFMDDKKLLIGD LS+KLI+AIEKS SFIVVLS+NYASSK
Sbjct: 13  DTRNTFTGHLYKELVGLGITTFMDDKKLLIGDSLSEKLIKAIEKSDSFIVVLSENYASSK 72

Query: 62  WCLRELTKIIDCMAEQKHRV-LPVFYHVSPRDVRRQSGCFEKSFHEHEKILLELDNMERD 121
           WCLREL KIIDC  EQKHRV LPVFYHV+P DVRRQSGCFE SF  HE++L ELD+MERD
Sbjct: 73  WCLRELAKIIDCTDEQKHRVLLPVFYHVNPHDVRRQSGCFENSFRLHEELLRELDHMERD 132

Query: 122 KYMKEVQQWRRALTKVGDLSGEVA--NIVEVVSIGKITKQLFDM-LHHQKLVASDKLNQL 181
           KYM+EVQQWRRA TKVGDL+G V   + VEV SIGKIT QL DM LHHQKLV  D+L +L
Sbjct: 133 KYMEEVQQWRRAFTKVGDLTGVVVTKDSVEVASIGKITNQLLDMLLHHQKLVPWDELTKL 192

Query: 182 VDIERRLCKMDMLIDLKSNKVHFVGIIGMGGIGKTTIAEVFYDKVAYKFGKNRCFLRTYE 241
           VDIER+L KM+ L DL+ N V F+GIIGMGGIGKTTIAEVFYD+VA  FGKNRCFLR YE
Sbjct: 193 VDIERQLFKMEKLNDLEPNVVRFIGIIGMGGIGKTTIAEVFYDRVARIFGKNRCFLRIYE 252

Query: 242 H-TLVSLQQQLLSQLLQTKDIIINNENEGATMIGSYLKEKKVLIVLDGVKDKSQLEKLVG 301
           H TL+SLQQQLLSQLLQTKD+IINNENEGA MIGS LK+K+VLIVLDGVK+KSQLE+LVG
Sbjct: 253 HTTLLSLQQQLLSQLLQTKDLIINNENEGARMIGSRLKDKRVLIVLDGVKEKSQLEQLVG 312

Query: 302 SPNWFGQGSQIIITTRNRDVLRQPNYKDKMVEYNVEFLDYKSAMSLFCKHAFGCEFPNKN 361
           +PNWFG GS+IIITTRNRDVLRQPNYKDKMVEY++EFLD KSAM+LFCKHAFGC FP+KN
Sbjct: 313 NPNWFGSGSKIIITTRNRDVLRQPNYKDKMVEYSMEFLDTKSAMTLFCKHAFGCGFPSKN 372

Query: 362 YEDFCKEIVERVKGHPQALIQIGLSLYDQDIDIWKEELKNLREDYNKHLFKTLKISFDDL 421
           +EDF KEIVERVKGHPQALIQIG SLYD+ I+IWKEELK+L EDYN  +FKTLKISFDDL
Sbjct: 373 FEDFSKEIVERVKGHPQALIQIGSSLYDKGIEIWKEELKSLEEDYNNRIFKTLKISFDDL 432

Query: 422 GKTSQEAFLDLACFFNGKKKEKVIEILKSFDYRPHSEIQLLQDRCLIEVRRDNSIFMPKC 481
            KTSQE FLDLACFFN K KEKVIEILKSFDYRPHSEIQLLQDRCLIEVR DN+IFMPKC
Sbjct: 433 EKTSQEVFLDLACFFNEKTKEKVIEILKSFDYRPHSEIQLLQDRCLIEVRSDNTIFMPKC 492

Query: 482 IQAMGQEIEREADKRSRIWLPKDALDVFDEGHRVKEIKGIVLEGEEKQDEVELEGRAFKE 541
           IQ MGQ+IEREADKRSRIW+PKDA DVFDE HRVK+IKG+VL+ EEKQDE+ELEG+ F++
Sbjct: 493 IQTMGQQIEREADKRSRIWIPKDAQDVFDEPHRVKDIKGVVLKLEEKQDEIELEGKVFED 552

Query: 542 MTCLKILEMSNVQLRGDFTHLSKQLRLLNWHNYPSQCLPLRFESRYLFELLLPLSQTRQL 601
           M  LKILE+ NV++ GDFTHLSKQLRLLNWH+YPSQCLPL FESRYLF+LLLPLSQTRQL
Sbjct: 553 MRSLKILEIGNVEVSGDFTHLSKQLRLLNWHSYPSQCLPLSFESRYLFQLLLPLSQTRQL 612

Query: 602 WNGQ------KVINISDSKNLRETPNFTKVPNLESLVLSNCTRLWKIDSSISRLNRLTLL 661
           WNGQ      KVIN+S SKNLRETPNFTKVPNLESL LSNCTRLWKIDSSISRLNRLTLL
Sbjct: 613 WNGQKGFEKLKVINVSGSKNLRETPNFTKVPNLESLDLSNCTRLWKIDSSISRLNRLTLL 672

Query: 662 DLTCCINLKSIPSIKSCKSLTTLNLAGSGL--IGLEHSQN----VFQLAKRRS 698
           D+TCCINLK++   +SCKSL T+N  GSGL   GL++ QN    ++   KRRS
Sbjct: 673 DITCCINLKNLSFSRSCKSLITINYVGSGLEEKGLDNFQNDDSQLYFQPKRRS 725

BLAST of Lsi08G001330 vs. ExPASy TrEMBL
Match: Q5DMW2 (MRGH9 OS=Cucumis melo OX=3656 GN=MRGH9 PE=4 SV=1)

HSP 1 Score: 1097.0 bits (2836), Expect = 0.0e+00
Identity = 556/690 (80.58%), Postives = 615/690 (89.13%), Query Frame = 0

Query: 2   NTRNTFTGYLYKELVELGISTFMDDKKLLIGDRLSKKLIRAIEKSGSFIVVLSKNYASSK 61
           +TRNTFTG+LYKELV LGI+TFMDDKKLLIGD LS+KLI+AIEKS SFIVVLS+NYASSK
Sbjct: 13  DTRNTFTGHLYKELVGLGITTFMDDKKLLIGDSLSEKLIKAIEKSDSFIVVLSENYASSK 72

Query: 62  WCLRELTKIIDCMAEQKHRV-LPVFYHVSPRDVRRQSGCFEKSFHEHEKILLELDNMERD 121
           WCLREL KIIDC  EQKHRV LPVFYHV+P DVRRQSGCFE SF  HE++L ELD+MERD
Sbjct: 73  WCLRELAKIIDCTDEQKHRVLLPVFYHVNPHDVRRQSGCFENSFRLHEELLRELDHMERD 132

Query: 122 KYMKEVQQWRRALTKVGDLSGEVA--NIVEVVSIGKITKQLFDM-LHHQKLVASDKLNQL 181
           KYM+EVQQWRRA TKVGDL+G V   + VEV SIGKIT QL DM LHHQKLV  D+L +L
Sbjct: 133 KYMEEVQQWRRAFTKVGDLTGVVVTKDSVEVASIGKITNQLLDMLLHHQKLVPWDELTKL 192

Query: 182 VDIERRLCKMDMLIDLKSNKVHFVGIIGMGGIGKTTIAEVFYDKVAYKFGKNRCFLRTYE 241
           VDIER+L KM+ L DL+ N V F+GIIGMGGIGKTTIAEVFYD+VA  FGKNRCFLR YE
Sbjct: 193 VDIERQLFKMEKLNDLEPNVVRFIGIIGMGGIGKTTIAEVFYDRVARIFGKNRCFLRIYE 252

Query: 242 H-TLVSLQQQLLSQLLQTKDIIINNENEGATMIGSYLKEKKVLIVLDGVKDKSQLEKLVG 301
           H TL+SLQQQLLSQLLQTKD+IINNENEGA MIGS LK+K+VLIVLDGVK+KSQL++LVG
Sbjct: 253 HTTLLSLQQQLLSQLLQTKDLIINNENEGARMIGSRLKDKRVLIVLDGVKEKSQLDQLVG 312

Query: 302 SPNWFGQGSQIIITTRNRDVLRQPNYKDKMVEYNVEFLDYKSAMSLFCKHAFGCEFPNKN 361
           +PNWFG GS+IIITTRNRDVLRQPNYKDKMVEY++EFLD KSAM+LFCKHAFGC FP+KN
Sbjct: 313 NPNWFGSGSKIIITTRNRDVLRQPNYKDKMVEYSMEFLDTKSAMTLFCKHAFGCGFPSKN 372

Query: 362 YEDFCKEIVERVKGHPQALIQIGLSLYDQDIDIWKEELKNLREDYNKHLFKTLKISFDDL 421
           +EDF KEIVERVKGHPQALIQIG SLYD+ I+IWKEELK+L EDYN  +FKTLKISFDDL
Sbjct: 373 FEDFSKEIVERVKGHPQALIQIGSSLYDKGIEIWKEELKSLEEDYNNRIFKTLKISFDDL 432

Query: 422 GKTSQEAFLDLACFFNGKKKEKVIEILKSFDYRPHSEIQLLQDRCLIEVRRDNSIFMPKC 481
            KTSQE FLDLACFFN K KEKVIEILKSFDYRPHSEIQLLQDRCLIEVR DN+IFMPKC
Sbjct: 433 EKTSQEVFLDLACFFNEKTKEKVIEILKSFDYRPHSEIQLLQDRCLIEVRSDNTIFMPKC 492

Query: 482 IQAMGQEIEREADKRSRIWLPKDALDVFDEGHRVKEIKGIVLEGEEKQDEVELEGRAFKE 541
           IQ MGQ+IEREADKRSRIW+PKDA DVFDE HRVK+IKG+VL+ EEKQDE+ELEG+ F++
Sbjct: 493 IQTMGQQIEREADKRSRIWIPKDAQDVFDEPHRVKDIKGVVLKLEEKQDEIELEGKVFED 552

Query: 542 MTCLKILEMSNVQLRGDFTHLSKQLRLLNWHNYPSQCLPLRFESRYLFELLLPLSQTRQL 601
           M  LKILE+ NV++ GDFTHLSKQLRLLNWH+YPSQCLPL FESRYLF+LLLPLSQTRQL
Sbjct: 553 MRSLKILEIGNVEVSGDFTHLSKQLRLLNWHSYPSQCLPLSFESRYLFQLLLPLSQTRQL 612

Query: 602 WNGQ------KVINISDSKNLRETPNFTKVPNLESLVLSNCTRLWKIDSSISRLNRLTLL 661
           WNGQ      KVIN+S SKNLRETPNFTKVPNLESL LSNCTRLWKIDSSISRLNRLTLL
Sbjct: 613 WNGQKGFEKLKVINVSGSKNLRETPNFTKVPNLESLDLSNCTRLWKIDSSISRLNRLTLL 672

Query: 662 DLTCCINLKSIPSIKSCKSLTTLNLAGSGL 681
           D+TCCINLK++   +SCKSL T+N  GSGL
Sbjct: 673 DITCCINLKNLSFSRSCKSLITINYVGSGL 702

BLAST of Lsi08G001330 vs. ExPASy TrEMBL
Match: M4QSV9 (Fom-1 OS=Cucumis melo OX=3656 GN=Fom-1 PE=4 SV=1)

HSP 1 Score: 1082.8 bits (2799), Expect = 0.0e+00
Identity = 553/691 (80.03%), Postives = 610/691 (88.28%), Query Frame = 0

Query: 2   NTRNTFTGYLYKELVELGISTFMDDKKLLIGDRLSKKLIRAIEKSGSFIVVLSKNYASSK 61
           +TRNTFTG+LYKELV LGI+TFMDDKKL IGD LS+KLI+AIEKS SFIVVLS+NYASSK
Sbjct: 13  DTRNTFTGHLYKELVGLGITTFMDDKKLFIGDSLSEKLIKAIEKSDSFIVVLSENYASSK 72

Query: 62  WCLRELTKIIDCMAEQKHRV-LPVFYHVSPRDVRRQSGCFEKSFHEHEKILLELDNMERD 121
           WCLREL KIIDC  EQKHRV LPVFYHV+P DVR QSGCFE SF  HE++L ELD+MERD
Sbjct: 73  WCLRELAKIIDCTDEQKHRVLLPVFYHVNPHDVRHQSGCFENSFRLHEELLRELDHMERD 132

Query: 122 KYMKEVQQWRRALTKVGDLSGEVA--NIVEVVSIGKITKQLFDMLHHQKLVASDKLNQLV 181
           KYM+EVQQWRRA TKVGDL+G V   + VEV SIGKIT QL DML   KLV  D+L +LV
Sbjct: 133 KYMEEVQQWRRAFTKVGDLTGVVVTKDSVEVDSIGKITNQLLDML---KLVPWDELTKLV 192

Query: 182 DIERRLCKMDMLIDLKSNKVHFVGIIGMGGIGKTTIAEVFYDKVAYKFGKNRCFLRTYEH 241
           DIER+L KM+ L DL+ N V F+GIIGMGGIGKTTIAEVFYD+VA  FGKNRCFLR YEH
Sbjct: 193 DIERQLFKMEKLNDLEPNVVRFIGIIGMGGIGKTTIAEVFYDRVARTFGKNRCFLRIYEH 252

Query: 242 -TLVSLQQQLLSQLLQTKDIIINNENEGATMIGSYLKEKKVLIVLDGVKDKSQLEKLVGS 301
            TL+SLQQQLLSQLLQTKD+IINNENEGA MIGS LK+K+VLIVLDGVK+K+QLE+LVG+
Sbjct: 253 TTLLSLQQQLLSQLLQTKDLIINNENEGARMIGSRLKDKRVLIVLDGVKEKTQLEQLVGN 312

Query: 302 PNWFGQGSQIIITTRNRDVLRQPNYKDKMVEYNVEFLDYKSAMSLFCKHAFGCEFPNKNY 361
           PNWFG GS+IIITTRNRDVLRQPNYKDKMVEY+VEFLD KSAM+LFCKHAFGC FP KN+
Sbjct: 313 PNWFGSGSKIIITTRNRDVLRQPNYKDKMVEYSVEFLDTKSAMTLFCKHAFGCGFPRKNF 372

Query: 362 EDFCKEIVERVKGHPQALIQIGLSLYDQDIDIWKEELKNLREDYNKHLFKTLKISFDDLG 421
           +DF KEIVERVKGHPQALIQIG SLYD+ I+IWKEELK+L EDYN  +FKTLKISFDDLG
Sbjct: 373 KDFSKEIVERVKGHPQALIQIGSSLYDKGIEIWKEELKSLEEDYNNRIFKTLKISFDDLG 432

Query: 422 KTSQEAFLDLACFFNGKKKEKVIEILKSFDYRPHSEIQLLQDRCLIEVRRDNSIFMPKCI 481
           KTSQE FLDLACFFN K KEKVIEILKSFDYRPHSEIQLLQDRCLIEVR DN+I MPKCI
Sbjct: 433 KTSQEVFLDLACFFNEKTKEKVIEILKSFDYRPHSEIQLLQDRCLIEVRSDNTILMPKCI 492

Query: 482 QAMGQEIEREADKRSRIWLPKDALDVFDEGH-RVKEIKGIVLEGEEKQDEVELEGRAFKE 541
           Q MGQ+IEREADKRSRIWLPKDA DVFDE H RVK+IKG+VL+ EEKQDE+ELEG+ F++
Sbjct: 493 QTMGQQIEREADKRSRIWLPKDAQDVFDEPHQRVKDIKGVVLKLEEKQDEIELEGKVFED 552

Query: 542 MTCLKILEMSNVQLRGDFTHLSKQLRLLNWHNYPSQCLPLRFESRYLFELLLPLSQTRQL 601
           M  LKILE+ NV++ GDFTHLSKQLRLLNWH+YPSQCLPL FESRYLF+LLLPLSQTRQL
Sbjct: 553 MRSLKILEIGNVEVSGDFTHLSKQLRLLNWHSYPSQCLPLSFESRYLFQLLLPLSQTRQL 612

Query: 602 WNGQ-------KVINISDSKNLRETPNFTKVPNLESLVLSNCTRLWKIDSSISRLNRLTL 661
           WNGQ       KVIN+S SKNLRETPNFTKVPNLESL LSNCTRLWKIDSSISRLNRLTL
Sbjct: 613 WNGQKEGFEKLKVINVSGSKNLRETPNFTKVPNLESLDLSNCTRLWKIDSSISRLNRLTL 672

Query: 662 LDLTCCINLKSIPSIKSCKSLTTLNLAGSGL 681
           LD+TCCINLK++P  +SCKSL T+N  GSGL
Sbjct: 673 LDITCCINLKNLPFSRSCKSLITINYVGSGL 700

BLAST of Lsi08G001330 vs. ExPASy TrEMBL
Match: A0A6J1CK85 (TMV resistance protein N-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111012052 PE=4 SV=1)

HSP 1 Score: 958.4 bits (2476), Expect = 1.8e-275
Identity = 498/711 (70.04%), Postives = 572/711 (80.45%), Query Frame = 0

Query: 2   NTRNTFTGYLYKELVELGISTFMDDKKLLIGDRLSKKLIRAIEKSGSFIVVLSKNYASSK 61
           +TRNTF GYLYKEL +LGI TF DD  LLIGD +S KL+RAIEKS S IVVLSK+YASSK
Sbjct: 23  DTRNTFIGYLYKELHQLGIMTFKDDNSLLIGDNISNKLVRAIEKSRSSIVVLSKDYASSK 82

Query: 62  WCLRELTKIIDCMAEQKHRVLPVFYHVSPRDVRRQSGCFEKSFHEHEKILLELDNMERDK 121
           WCLREL KIIDCM +  HRVLPVFYHV P DVR QSG F+K F E+E+ L +  N E+ K
Sbjct: 83  WCLRELAKIIDCMVQCSHRVLPVFYHVDPSDVRHQSGSFKKCFDEYEETLQQFMNYEQ-K 142

Query: 122 YMKEVQQWRRALTKVGDLSGEVA--NIVEVVSIGKITKQLFDMLHHQKLVASDKLNQLVD 181
              EVQQWR A+TK+ +L+G V     VEV SI KITKQ+FDMLH+ KLVA+DKLN LVD
Sbjct: 143 EEHEVQQWREAMTKIANLTGVVVTKESVEVASIDKITKQVFDMLHYPKLVAADKLNNLVD 202

Query: 182 IERRLCKMDMLIDLKSNKVHFVGIIGMGGIGKTTIAEVFYDKVAYKFGKNRCFLRTYEH- 241
           IERRL  MD LI L+SN V FVGIIGMGGIGKTTIAEVFYDKVA+KFGKNRCFLR   H 
Sbjct: 203 IERRLYSMDTLIGLESNDVCFVGIIGMGGIGKTTIAEVFYDKVAHKFGKNRCFLRIQGHS 262

Query: 242 -TLVSLQQQLLSQLLQTKDIIINNENEGATMIGSYLKEKKVLIVLDGVKDKSQLEKLVGS 301
            +L+SLQ QLLSQLL+ +DI I +ENEG  MI  +L  KKVLIVLDG+ +K QLEKL GS
Sbjct: 263 LSLISLQHQLLSQLLRKEDIKIMDENEGEYMIKYHLSGKKVLIVLDGIDEKGQLEKLAGS 322

Query: 302 PNWFGQGSQIIITTRNRDVLRQPNYKDKMVEYNVEFLDYKSAMSLFCKHAFGCE-FPNKN 361
           P+WFG  SQIIITTRNRDVLRQ NYKDK++EY VE LDYKS  SLFCKHAFG    PN+N
Sbjct: 323 PDWFGPKSQIIITTRNRDVLRQSNYKDKLLEYKVELLDYKSGFSLFCKHAFGDHTCPNEN 382

Query: 362 YEDFCKEIVERVKGHPQALIQIGLSLYDQDIDIWKEELKNLREDYNKHLFKTLKISFDDL 421
           +E   KEIV+RVKGHPQAL+QIG  LY QDID+WKEEL +L  DYNKH+FKTLKISFD L
Sbjct: 383 FEQLSKEIVDRVKGHPQALVQIGSYLYGQDIDVWKEELNSLDIDYNKHVFKTLKISFDGL 442

Query: 422 GKTSQEAFLDLACFFNGKKKEKVIEILKSFDYRPHSEIQLLQDRCLIEVRRDNSIFMPKC 481
            KTSQ+ FLDLACFFNG++K+KVIEIL+SFDYRPHSEIQLL DRCLIEVRRD +IF+P C
Sbjct: 443 EKTSQQVFLDLACFFNGRRKDKVIEILRSFDYRPHSEIQLLYDRCLIEVRRDGTIFIPNC 502

Query: 482 IQAMGQEIEREADKRSRIWLPKDALDVFDEGHRVKEIKGIVLEGEEKQDEVELEGRAFKE 541
           IQ MG++IER+AD+RSRIW+ KD LDVFD+ H VK I+GIVL+ EEKQD+++L+   F++
Sbjct: 503 IQTMGRKIERDADERSRIWIRKDVLDVFDKRHGVKHIEGIVLDLEEKQDKLKLQAGLFED 562

Query: 542 MTCLKILEMSNVQLRGDFTHLSKQLRLLNWHNYPSQCLPLRFESRYLFELLLPLSQTRQL 601
           MT L+ILE+ NV+   DFTHLSKQLRLLNWH YPS CLPL+FESRYLFELLLP+ Q  QL
Sbjct: 563 MTGLRILEIGNVEFCEDFTHLSKQLRLLNWHGYPSTCLPLQFESRYLFELLLPVGQNTQL 622

Query: 602 WNGQ------KVINISDSKNLRETPNFTKVPNLESLVLSNCTRLWKIDSSISRLNRLTLL 661
           W GQ      KVI++SDS+NLRETPNFTKVPNLESLVL NCTRL+ ID SISRLNRLT L
Sbjct: 623 WKGQKGFEKLKVIDVSDSRNLRETPNFTKVPNLESLVLRNCTRLYMIDHSISRLNRLTSL 682

Query: 662 DLTCCINLKSIPSIKSCKSLTTLNLAGSGLIGLE-----HSQNVFQLAKRR 697
           DLTCC++L+S   I SCKSLTTL LAGSGL  L+     +S N+    KRR
Sbjct: 683 DLTCCVSLRSFSVINSCKSLTTLKLAGSGLERLKGVDNTYSPNILPPVKRR 732

BLAST of Lsi08G001330 vs. ExPASy TrEMBL
Match: A0A6J1CJ23 (TMV resistance protein N-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111012052 PE=4 SV=1)

HSP 1 Score: 955.3 bits (2468), Expect = 1.5e-274
Identity = 500/725 (68.97%), Postives = 576/725 (79.45%), Query Frame = 0

Query: 2   NTRNTFTGYLYKELVELGISTFMDDKKLLIGDRLSKKLIRAIEKSGSFIVVLSKNYASSK 61
           +TRNTF GYLYKEL +LGI TF DD  LLIGD +S KL+RAIEKS S IVVLSK+YASSK
Sbjct: 23  DTRNTFIGYLYKELHQLGIMTFKDDNSLLIGDNISNKLVRAIEKSRSSIVVLSKDYASSK 82

Query: 62  WCLRELTKIIDCMAEQKHRVLPVFYHVSPRDVRRQSGCFEKSFHEHEKILLELDNMERDK 121
           WCLREL KIIDCM +  HRVLPVFYHV P DVR QSG F+K F E+E+ L +  N E+ K
Sbjct: 83  WCLRELAKIIDCMVQCSHRVLPVFYHVDPSDVRHQSGSFKKCFDEYEETLQQFMNYEQ-K 142

Query: 122 YMKEVQQWRRALTKVGDLSGEVA--NIVEVVSIGKITKQLFDMLHHQKLVASDKLNQLVD 181
              EVQQWR A+TK+ +L+G V     VEV SI KITKQ+FDMLH+ KLVA+DKLN LVD
Sbjct: 143 EEHEVQQWREAMTKIANLTGVVVTKESVEVASIDKITKQVFDMLHYPKLVAADKLNNLVD 202

Query: 182 IERRLCKMDMLIDLKSNKVHFVGIIGMGGIGKTTIAEVFYDKVAYKFGKNRCFLRTYEH- 241
           IERRL  MD LI L+SN V FVGIIGMGGIGKTTIAEVFYDKVA+KFGKNRCFLR   H 
Sbjct: 203 IERRLYSMDTLIGLESNDVCFVGIIGMGGIGKTTIAEVFYDKVAHKFGKNRCFLRIQGHS 262

Query: 242 -TLVSLQQQLLSQLLQTKDIIINNENEGATMIGSYLKEKKVLIVLDGVKDKSQLEKLVGS 301
            +L+SLQ QLLSQLL+ +DI I +ENEG  MI  +L  KKVLIVLDG+ +K QLEKL GS
Sbjct: 263 LSLISLQHQLLSQLLRKEDIKIMDENEGEYMIKYHLSGKKVLIVLDGIDEKGQLEKLAGS 322

Query: 302 PNWFGQGSQIIITTRNRDVLRQPNYKDKMVEYNVEFLDYKSAMSLFCKHAFGCE-FPNKN 361
           P+WFG  SQIIITTRNRDVLRQ NYKDK++EY VE LDYKS  SLFCKHAFG    PN+N
Sbjct: 323 PDWFGPKSQIIITTRNRDVLRQSNYKDKLLEYKVELLDYKSGFSLFCKHAFGDHTCPNEN 382

Query: 362 YEDFCKEIVERVKGHPQALIQIGLSLYDQDIDIWKEELKNLREDYNKHLFKTLKISFDDL 421
           +E   KEIV+RVKGHPQAL+QIG  LY QDID+WKEEL +L  DYNKH+FKTLKISFD L
Sbjct: 383 FEQLSKEIVDRVKGHPQALVQIGSYLYGQDIDVWKEELNSLDIDYNKHVFKTLKISFDGL 442

Query: 422 GKTSQEAFLDLACFFNGKKKEKVIEILKSFDYRPHSEIQLLQDRCLIEVRRDNSIFMPKC 481
            KTSQ+ FLDLACFFNG++K+KVIEIL+SFDYRPHSEIQLL DRCLIEVRRD +IF+P C
Sbjct: 443 EKTSQQVFLDLACFFNGRRKDKVIEILRSFDYRPHSEIQLLYDRCLIEVRRDGTIFIPNC 502

Query: 482 IQAMGQEIEREADKRSRIWLPKDALDVFDEGHRVKEIKGIVLEGEEKQDEVELEGRAFKE 541
           IQ MG++IER+AD+RSRIW+ KD LDVFD+ H VK I+GIVL+ EEKQD+++L+   F++
Sbjct: 503 IQTMGRKIERDADERSRIWIRKDVLDVFDKRHGVKHIEGIVLDLEEKQDKLKLQAGLFED 562

Query: 542 MTCLKILEMSNVQLRGDFTHLSKQLRLLNWHNYPSQCLPLRFESRYLFELLLPLSQTRQL 601
           MT L+ILE+ NV+   DFTHLSKQLRLLNWH YPS CLPL+FESRYLFELLLP+ Q  QL
Sbjct: 563 MTGLRILEIGNVEFCEDFTHLSKQLRLLNWHGYPSTCLPLQFESRYLFELLLPVGQNTQL 622

Query: 602 WNGQ------KVINISDSKNLRETPNFTKVPNLESLVLSNCTRLWKIDSSISRLNRLTLL 661
           W GQ      KVI++SDS+NLRETPNFTKVPNLESLVL NCTRL+ ID SISRLNRLT L
Sbjct: 623 WKGQKGFEKLKVIDVSDSRNLRETPNFTKVPNLESLVLRNCTRLYMIDHSISRLNRLTSL 682

Query: 662 DLTCCINLKSIPSIKSCKSLTTLNLAGSGLIGLE-HSQNVFQLAKRRSTIISSPIDQDLY 715
           DLTCC++L+S   I SCKSLTTL LAGSGL  L+  ++N          I  SP   + Y
Sbjct: 683 DLTCCVSLRSFSVINSCKSLTTLKLAGSGLERLKAANKNFCPKVLHGDEISLSPCVDNTY 742

BLAST of Lsi08G001330 vs. NCBI nr
Match: XP_038891076.1 (disease resistance protein RPV1-like isoform X3 [Benincasa hispida] >XP_038891077.1 disease resistance protein RPV1-like isoform X3 [Benincasa hispida])

HSP 1 Score: 1179.9 bits (3051), Expect = 0.0e+00
Identity = 600/715 (83.92%), Postives = 652/715 (91.19%), Query Frame = 0

Query: 2   NTRNTFTGYLYKELVELGISTFMDDKKLLIGDRLSKKLIRAIEKSGSFIVVLSKNYASSK 61
           +TRNTFTG+LYKELVELGISTFMDDKKLLIGD LS+KLIRAIEKS SFIVVLS+NYASSK
Sbjct: 13  DTRNTFTGHLYKELVELGISTFMDDKKLLIGDSLSEKLIRAIEKSWSFIVVLSENYASSK 72

Query: 62  WCLRELTKIIDCMAEQKHRVLPVFYHVSPRDVRRQSGCFEKSFHEHEKILLELDNMERDK 121
           WCLREL KIIDCMA QKHRV+PVFYHV+PRDVR Q GCF+KSF +HE+IL EL+NMERDK
Sbjct: 73  WCLRELVKIIDCMAVQKHRVIPVFYHVNPRDVRHQLGCFKKSFRKHEEILQELNNMERDK 132

Query: 122 YMKEVQQWRRALTKVGDLSGEVA--NIVEVVSIGKITKQLFDMLHHQKLVASDKLNQLVD 181
           YMKEVQQWRRALTKVGDLSG V   + VEV SIGKITKQL D+L+HQ LVASDK+++LVD
Sbjct: 133 YMKEVQQWRRALTKVGDLSGVVVTKDSVEVASIGKITKQLLDILNHQMLVASDKVDRLVD 192

Query: 182 IERRLCKMDMLIDLKSNKVHFVGIIGMGGIGKTTIAEVFYDKVAYKFGKNRCFLRTYEHT 241
           +ERRL KMD LIDL+SN V FVGI GMGGIGKTTIAEVFYDKVA KFGKN CFLR YE T
Sbjct: 193 MERRLSKMDELIDLESNDVRFVGITGMGGIGKTTIAEVFYDKVANKFGKNHCFLRIYERT 252

Query: 242 LVSLQQQLLSQLLQTKDIIINNENEGATMIGSYLKEKKVLIVLDGVKDKSQLEKLVGSPN 301
           LVSLQQQLLSQLL+TKDI+INNENEGA MI SYLK KKV I LDGVK+ SQLE LVGSP 
Sbjct: 253 LVSLQQQLLSQLLRTKDIVINNENEGARMIESYLKGKKVFIALDGVKEISQLEMLVGSPI 312

Query: 302 WFGQGSQIIITTRNRDVLRQPNYKDKMVEYNVEFLDYKSAMSLFCKHAFGCEFPNKNYED 361
           WFGQGS+IIITTRNRDVLRQPNY+D MVEYNVEFLDY+SAMSLFCKHAFG EFPN+N+ED
Sbjct: 313 WFGQGSKIIITTRNRDVLRQPNYRDIMVEYNVEFLDYESAMSLFCKHAFGSEFPNRNFED 372

Query: 362 FCKEIVERVKGHPQALIQIGLSLYDQDIDIWKEELKNLREDY-NKHLFKTLKISFDDLGK 421
             KEIVERVKGHPQALIQIGLSLYD+ IDIWKEELK+L +DY NKHLFKTLKISFDDLGK
Sbjct: 373 LSKEIVERVKGHPQALIQIGLSLYDKGIDIWKEELKSLEKDYMNKHLFKTLKISFDDLGK 432

Query: 422 TSQEAFLDLACFFNGKKKEKVIEILKSFDYRPHSEIQLLQDRCLIEVRRDNSIFMPKCIQ 481
           TSQE FLDLACFFNGKKKEKVIEILKSFDYRPHSE++LL+DRCLIEVRRDN+IFMP CIQ
Sbjct: 433 TSQEVFLDLACFFNGKKKEKVIEILKSFDYRPHSELKLLEDRCLIEVRRDNTIFMPNCIQ 492

Query: 482 AMGQEIEREADKRSRIWLPKDALDVFDEGHRVKEIKGIVLEGEEKQDEVELEGRAFKEMT 541
           AMGQEIER ADKRSRIWLPKDA+D FD+ HRV++IKGIVLE EEKQDEVELEG+ F++MT
Sbjct: 493 AMGQEIERGADKRSRIWLPKDAIDAFDQRHRVRDIKGIVLEMEEKQDEVELEGKVFEDMT 552

Query: 542 CLKILEMSNVQLRGDFTHLSKQLRLLNWHNYPSQCLPLRFESRYLFELLLPLSQTRQLWN 601
           CLKIL++SNV++ GDFTHLSKQLRLLNWH+YPS+CLPLRF+SRYLF+LLLPLSQTRQLWN
Sbjct: 553 CLKILQISNVKVIGDFTHLSKQLRLLNWHSYPSECLPLRFDSRYLFQLLLPLSQTRQLWN 612

Query: 602 GQ------KVINISDSKNLRETPNFTKVPNLESLVLSNCTRLWKIDSSISRLNRLTLLDL 661
           GQ      K+IN+SDSKNLRETPNFTKVPNLESLVLSNCTRLWKIDSSISRLNRLTLLDL
Sbjct: 613 GQKGFEKLKLINVSDSKNLRETPNFTKVPNLESLVLSNCTRLWKIDSSISRLNRLTLLDL 672

Query: 662 TCCINLKSIPSIKSCKSLTTLNLAGSGL-----IGLEHSQNVFQLAKRRSTIISS 703
           TCCINLKS+PSI+SCKSLTTLNLAGSGL      GLEH QNVFQLAKRRST I S
Sbjct: 673 TCCINLKSLPSIQSCKSLTTLNLAGSGLECLDQKGLEHFQNVFQLAKRRSTRIPS 727

BLAST of Lsi08G001330 vs. NCBI nr
Match: XP_038891073.1 (disease resistance protein RPV1-like isoform X2 [Benincasa hispida] >XP_038891074.1 disease resistance protein RPV1-like isoform X2 [Benincasa hispida] >XP_038891075.1 disease resistance protein RPV1-like isoform X2 [Benincasa hispida])

HSP 1 Score: 1174.8 bits (3038), Expect = 0.0e+00
Identity = 600/717 (83.68%), Postives = 652/717 (90.93%), Query Frame = 0

Query: 2   NTRNTFTGYLYKELVELGISTFMDDKKLLIGDRLSKKLIRAIEKSGSFIVVLSKNYASSK 61
           +TRNTFTG+LYKELVELGISTFMDDKKLLIGD LS+KLIRAIEKS SFIVVLS+NYASSK
Sbjct: 13  DTRNTFTGHLYKELVELGISTFMDDKKLLIGDSLSEKLIRAIEKSWSFIVVLSENYASSK 72

Query: 62  WCLRELTKIIDCMAEQKHRVLPVFYHVSPRDVRRQSGCFEKSFHEHEKILLELDNMERDK 121
           WCLREL KIIDCMA QKHRV+PVFYHV+PRDVR Q GCF+KSF +HE+IL EL+NMERDK
Sbjct: 73  WCLRELVKIIDCMAVQKHRVIPVFYHVNPRDVRHQLGCFKKSFRKHEEILQELNNMERDK 132

Query: 122 YMKEVQQWRRALTKVGDLSGEVA--NIVEVVSIGKITKQLFDMLHHQKLVASDKLNQLVD 181
           YMKEVQQWRRALTKVGDLSG V   + VEV SIGKITKQL D+L+HQ LVASDK+++LVD
Sbjct: 133 YMKEVQQWRRALTKVGDLSGVVVTKDSVEVASIGKITKQLLDILNHQMLVASDKVDRLVD 192

Query: 182 IERRLCKMDMLIDLKSNKVHFVGIIGMGGIGKTTIAEVFYDKVAYKFGKNRCFLRTYEHT 241
           +ERRL KMD LIDL+SN V FVGI GMGGIGKTTIAEVFYDKVA KFGKN CFLR YE T
Sbjct: 193 MERRLSKMDELIDLESNDVRFVGITGMGGIGKTTIAEVFYDKVANKFGKNHCFLRIYERT 252

Query: 242 LVSLQQQLLSQLLQTKDIIINNENEGATMIGSYLKEKKVLIVLDGVKDKSQLEKLVGSPN 301
           LVSLQQQLLSQLL+TKDI+INNENEGA MI SYLK KKV I LDGVK+ SQLE LVGSP 
Sbjct: 253 LVSLQQQLLSQLLRTKDIVINNENEGARMIESYLKGKKVFIALDGVKEISQLEMLVGSPI 312

Query: 302 WFGQGSQIIITTRNRDVLRQPNYKDKMVEYNVEFLDYKSAMSLFCKHAFGCEFPNKNYED 361
           WFGQGS+IIITTRNRDVLRQPNY+D MVEYNVEFLDY+SAMSLFCKHAFG EFPN+N+ED
Sbjct: 313 WFGQGSKIIITTRNRDVLRQPNYRDIMVEYNVEFLDYESAMSLFCKHAFGSEFPNRNFED 372

Query: 362 FCKEIVERVKGHPQALIQIGLSLYDQDIDIWKEELKNLREDY-NKHLFKTLKISFDDLGK 421
             KEIVERVKGHPQALIQIGLSLYD+ IDIWKEELK+L +DY NKHLFKTLKISFDDLGK
Sbjct: 373 LSKEIVERVKGHPQALIQIGLSLYDKGIDIWKEELKSLEKDYMNKHLFKTLKISFDDLGK 432

Query: 422 TSQEAFLDLACFFNGKKKEKVIEILKSFDYRPHSEIQLLQDRCLIEVRRDNSIFMPKCIQ 481
           TSQE FLDLACFFNGKKKEKVIEILKSFDYRPHSE++LL+DRCLIEVRRDN+IFMP CIQ
Sbjct: 433 TSQEVFLDLACFFNGKKKEKVIEILKSFDYRPHSELKLLEDRCLIEVRRDNTIFMPNCIQ 492

Query: 482 AMGQEIEREADKRSRIWLPKDALDVFDEGHRVKEIKGIVLEGEEKQDEVELEGRAFKEMT 541
           AMGQEIER ADKRSRIWLPKDA+D FD+ HRV++IKGIVLE EEKQDEVELEG+ F++MT
Sbjct: 493 AMGQEIERGADKRSRIWLPKDAIDAFDQRHRVRDIKGIVLEMEEKQDEVELEGKVFEDMT 552

Query: 542 CLKILEMSNVQLRGDFTHLSKQLRLLNWHNYPSQCLPLRFESRYLFELLLPLSQTRQLWN 601
           CLKIL++SNV++ GDFTHLSKQLRLLNWH+YPS+CLPLRF+SRYLF+LLLPLSQTRQLWN
Sbjct: 553 CLKILQISNVKVIGDFTHLSKQLRLLNWHSYPSECLPLRFDSRYLFQLLLPLSQTRQLWN 612

Query: 602 GQ------KVINISDSKNLRETPNFTKVPNLESLVLSNCTRLWKIDSSISRLNRLTLLDL 661
           GQ      K+IN+SDSKNLRETPNFTKVPNLESLVLSNCTRLWKIDSSISRLNRLTLLDL
Sbjct: 613 GQKGFEKLKLINVSDSKNLRETPNFTKVPNLESLVLSNCTRLWKIDSSISRLNRLTLLDL 672

Query: 662 TCCINLKSIPSIKSCKSLTTLNLAGSGL-----IGLEHSQNVFQL--AKRRSTIISS 703
           TCCINLKS+PSI+SCKSLTTLNLAGSGL      GLEH QNVFQL  AKRRST I S
Sbjct: 673 TCCINLKSLPSIQSCKSLTTLNLAGSGLECLDQKGLEHFQNVFQLFQAKRRSTRIPS 729

BLAST of Lsi08G001330 vs. NCBI nr
Match: XP_038891070.1 (disease resistance protein RPV1-like isoform X1 [Benincasa hispida] >XP_038891071.1 disease resistance protein RPV1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1168.3 bits (3021), Expect = 0.0e+00
Identity = 593/708 (83.76%), Postives = 645/708 (91.10%), Query Frame = 0

Query: 2   NTRNTFTGYLYKELVELGISTFMDDKKLLIGDRLSKKLIRAIEKSGSFIVVLSKNYASSK 61
           +TRNTFTG+LYKELVELGISTFMDDKKLLIGD LS+KLIRAIEKS SFIVVLS+NYASSK
Sbjct: 13  DTRNTFTGHLYKELVELGISTFMDDKKLLIGDSLSEKLIRAIEKSWSFIVVLSENYASSK 72

Query: 62  WCLRELTKIIDCMAEQKHRVLPVFYHVSPRDVRRQSGCFEKSFHEHEKILLELDNMERDK 121
           WCLREL KIIDCMA QKHRV+PVFYHV+PRDVR Q GCF+KSF +HE+IL EL+NMERDK
Sbjct: 73  WCLRELVKIIDCMAVQKHRVIPVFYHVNPRDVRHQLGCFKKSFRKHEEILQELNNMERDK 132

Query: 122 YMKEVQQWRRALTKVGDLSGEVA--NIVEVVSIGKITKQLFDMLHHQKLVASDKLNQLVD 181
           YMKEVQQWRRALTKVGDLSG V   + VEV SIGKITKQL D+L+HQ LVASDK+++LVD
Sbjct: 133 YMKEVQQWRRALTKVGDLSGVVVTKDSVEVASIGKITKQLLDILNHQMLVASDKVDRLVD 192

Query: 182 IERRLCKMDMLIDLKSNKVHFVGIIGMGGIGKTTIAEVFYDKVAYKFGKNRCFLRTYEHT 241
           +ERRL KMD LIDL+SN V FVGI GMGGIGKTTIAEVFYDKVA KFGKN CFLR YE T
Sbjct: 193 MERRLSKMDELIDLESNDVRFVGITGMGGIGKTTIAEVFYDKVANKFGKNHCFLRIYERT 252

Query: 242 LVSLQQQLLSQLLQTKDIIINNENEGATMIGSYLKEKKVLIVLDGVKDKSQLEKLVGSPN 301
           LVSLQQQLLSQLL+TKDI+INNENEGA MI SYLK KKV I LDGVK+ SQLE LVGSP 
Sbjct: 253 LVSLQQQLLSQLLRTKDIVINNENEGARMIESYLKGKKVFIALDGVKEISQLEMLVGSPI 312

Query: 302 WFGQGSQIIITTRNRDVLRQPNYKDKMVEYNVEFLDYKSAMSLFCKHAFGCEFPNKNYED 361
           WFGQGS+IIITTRNRDVLRQPNY+D MVEYNVEFLDY+SAMSLFCKHAFG EFPN+N+ED
Sbjct: 313 WFGQGSKIIITTRNRDVLRQPNYRDIMVEYNVEFLDYESAMSLFCKHAFGSEFPNRNFED 372

Query: 362 FCKEIVERVKGHPQALIQIGLSLYDQDIDIWKEELKNLREDY-NKHLFKTLKISFDDLGK 421
             KEIVERVKGHPQALIQIGLSLYD+ IDIWKEELK+L +DY NKHLFKTLKISFDDLGK
Sbjct: 373 LSKEIVERVKGHPQALIQIGLSLYDKGIDIWKEELKSLEKDYMNKHLFKTLKISFDDLGK 432

Query: 422 TSQEAFLDLACFFNGKKKEKVIEILKSFDYRPHSEIQLLQDRCLIEVRRDNSIFMPKCIQ 481
           TSQE FLDLACFFNGKKKEKVIEILKSFDYRPHSE++LL+DRCLIEVRRDN+IFMP CIQ
Sbjct: 433 TSQEVFLDLACFFNGKKKEKVIEILKSFDYRPHSELKLLEDRCLIEVRRDNTIFMPNCIQ 492

Query: 482 AMGQEIEREADKRSRIWLPKDALDVFDEGHRVKEIKGIVLEGEEKQDEVELEGRAFKEMT 541
           AMGQEIER ADKRSRIWLPKDA+D FD+ HRV++IKGIVLE EEKQDEVELEG+ F++MT
Sbjct: 493 AMGQEIERGADKRSRIWLPKDAIDAFDQRHRVRDIKGIVLEMEEKQDEVELEGKVFEDMT 552

Query: 542 CLKILEMSNVQLRGDFTHLSKQLRLLNWHNYPSQCLPLRFESRYLFELLLPLSQTRQLWN 601
           CLKIL++SNV++ GDFTHLSKQLRLLNWH+YPS+CLPLRF+SRYLF+LLLPLSQTRQLWN
Sbjct: 553 CLKILQISNVKVIGDFTHLSKQLRLLNWHSYPSECLPLRFDSRYLFQLLLPLSQTRQLWN 612

Query: 602 GQ------KVINISDSKNLRETPNFTKVPNLESLVLSNCTRLWKIDSSISRLNRLTLLDL 661
           GQ      K+IN+SDSKNLRETPNFTKVPNLESLVLSNCTRLWKIDSSISRLNRLTLLDL
Sbjct: 613 GQKGFEKLKLINVSDSKNLRETPNFTKVPNLESLVLSNCTRLWKIDSSISRLNRLTLLDL 672

Query: 662 TCCINLKSIPSIKSCKSLTTLNLAGSGL-----IGLEHSQNVFQLAKR 696
           TCCINLKS+PSI+SCKSLTTLNLAGSGL      GLEH QNVFQL  R
Sbjct: 673 TCCINLKSLPSIQSCKSLTTLNLAGSGLECLDQKGLEHFQNVFQLNLR 720

BLAST of Lsi08G001330 vs. NCBI nr
Match: XP_008459546.1 (PREDICTED: TMV resistance protein N-like [Cucumis melo])

HSP 1 Score: 1101.3 bits (2847), Expect = 0.0e+00
Identity = 565/713 (79.24%), Postives = 627/713 (87.94%), Query Frame = 0

Query: 2   NTRNTFTGYLYKELVELGISTFMDDKKLLIGDRLSKKLIRAIEKSGSFIVVLSKNYASSK 61
           +TRNTFTG+LYKELV LGI+TFMDDKKLLIGD LS+KLI+AIEKS SFIVVLS+NYASSK
Sbjct: 13  DTRNTFTGHLYKELVGLGITTFMDDKKLLIGDSLSEKLIKAIEKSDSFIVVLSENYASSK 72

Query: 62  WCLRELTKIIDCMAEQKHRV-LPVFYHVSPRDVRRQSGCFEKSFHEHEKILLELDNMERD 121
           WCLREL KIIDC  EQKHRV LPVFYHV+P DVRRQSGCFE SF  HE++L ELD+MERD
Sbjct: 73  WCLRELAKIIDCTDEQKHRVLLPVFYHVNPHDVRRQSGCFENSFRLHEELLRELDHMERD 132

Query: 122 KYMKEVQQWRRALTKVGDLSGEVA--NIVEVVSIGKITKQLFDM-LHHQKLVASDKLNQL 181
           KYM+EVQQWRRA TKVGDL+G V   + VEV SIGKIT QL DM LHHQKLV  D+L +L
Sbjct: 133 KYMEEVQQWRRAFTKVGDLTGVVVTKDSVEVASIGKITNQLLDMLLHHQKLVPWDELTKL 192

Query: 182 VDIERRLCKMDMLIDLKSNKVHFVGIIGMGGIGKTTIAEVFYDKVAYKFGKNRCFLRTYE 241
           VDIER+L KM+ L DL+ N V F+GIIGMGGIGKTTIAEVFYD+VA  FGKNRCFLR YE
Sbjct: 193 VDIERQLFKMEKLNDLEPNVVRFIGIIGMGGIGKTTIAEVFYDRVARIFGKNRCFLRIYE 252

Query: 242 H-TLVSLQQQLLSQLLQTKDIIINNENEGATMIGSYLKEKKVLIVLDGVKDKSQLEKLVG 301
           H TL+SLQQQLLSQLLQTKD+IINNENEGA MIGS LK+K+VLIVLDGVK+KSQLE+LVG
Sbjct: 253 HTTLLSLQQQLLSQLLQTKDLIINNENEGARMIGSRLKDKRVLIVLDGVKEKSQLEQLVG 312

Query: 302 SPNWFGQGSQIIITTRNRDVLRQPNYKDKMVEYNVEFLDYKSAMSLFCKHAFGCEFPNKN 361
           +PNWFG GS+IIITTRNRDVLRQPNYKDKMVEY++EFLD KSAM+LFCKHAFGC FP+KN
Sbjct: 313 NPNWFGSGSKIIITTRNRDVLRQPNYKDKMVEYSMEFLDTKSAMTLFCKHAFGCGFPSKN 372

Query: 362 YEDFCKEIVERVKGHPQALIQIGLSLYDQDIDIWKEELKNLREDYNKHLFKTLKISFDDL 421
           +EDF KEIVERVKGHPQALIQIG SLYD+ I+IWKEELK+L EDYN  +FKTLKISFDDL
Sbjct: 373 FEDFSKEIVERVKGHPQALIQIGSSLYDKGIEIWKEELKSLEEDYNNRIFKTLKISFDDL 432

Query: 422 GKTSQEAFLDLACFFNGKKKEKVIEILKSFDYRPHSEIQLLQDRCLIEVRRDNSIFMPKC 481
            KTSQE FLDLACFFN K KEKVIEILKSFDYRPHSEIQLLQDRCLIEVR DN+IFMPKC
Sbjct: 433 EKTSQEVFLDLACFFNEKTKEKVIEILKSFDYRPHSEIQLLQDRCLIEVRSDNTIFMPKC 492

Query: 482 IQAMGQEIEREADKRSRIWLPKDALDVFDEGHRVKEIKGIVLEGEEKQDEVELEGRAFKE 541
           IQ MGQ+IEREADKRSRIW+PKDA DVFDE HRVK+IKG+VL+ EEKQDE+ELEG+ F++
Sbjct: 493 IQTMGQQIEREADKRSRIWIPKDAQDVFDEPHRVKDIKGVVLKLEEKQDEIELEGKVFED 552

Query: 542 MTCLKILEMSNVQLRGDFTHLSKQLRLLNWHNYPSQCLPLRFESRYLFELLLPLSQTRQL 601
           M  LKILE+ NV++ GDFTHLSKQLRLLNWH+YPSQCLPL FESRYLF+LLLPLSQTRQL
Sbjct: 553 MRSLKILEIGNVEVSGDFTHLSKQLRLLNWHSYPSQCLPLSFESRYLFQLLLPLSQTRQL 612

Query: 602 WNGQ------KVINISDSKNLRETPNFTKVPNLESLVLSNCTRLWKIDSSISRLNRLTLL 661
           WNGQ      KVIN+S SKNLRETPNFTKVPNLESL LSNCTRLWKIDSSISRLNRLTLL
Sbjct: 613 WNGQKGFEKLKVINVSGSKNLRETPNFTKVPNLESLDLSNCTRLWKIDSSISRLNRLTLL 672

Query: 662 DLTCCINLKSIPSIKSCKSLTTLNLAGSGL--IGLEHSQN----VFQLAKRRS 698
           D+TCCINLK++   +SCKSL T+N  GSGL   GL++ QN    ++   KRRS
Sbjct: 673 DITCCINLKNLSFSRSCKSLITINYVGSGLEEKGLDNFQNDDSQLYFQPKRRS 725

BLAST of Lsi08G001330 vs. NCBI nr
Match: QQV74468.1 (resistance gene-like protein [Cucumis melo])

HSP 1 Score: 1098.6 bits (2840), Expect = 0.0e+00
Identity = 564/713 (79.10%), Postives = 626/713 (87.80%), Query Frame = 0

Query: 2   NTRNTFTGYLYKELVELGISTFMDDKKLLIGDRLSKKLIRAIEKSGSFIVVLSKNYASSK 61
           +TRNTFTG+LYKELV LGI+TFMDDKKLLIGD LS+KLI+AIE S SFIVVLS+NYASSK
Sbjct: 13  DTRNTFTGHLYKELVGLGITTFMDDKKLLIGDSLSEKLIKAIENSDSFIVVLSENYASSK 72

Query: 62  WCLRELTKIIDCMAEQKHRV-LPVFYHVSPRDVRRQSGCFEKSFHEHEKILLELDNMERD 121
           WCLREL KIIDC  EQKHRV LPVFYHV+PRDVRRQSGCFE SF  HE++L ELD+MERD
Sbjct: 73  WCLRELAKIIDCTDEQKHRVLLPVFYHVNPRDVRRQSGCFENSFRLHEELLRELDHMERD 132

Query: 122 KYMKEVQQWRRALTKVGDLSGEVA--NIVEVVSIGKITKQLFDM-LHHQKLVASDKLNQL 181
           KYM+EVQQWRRA TKVGDL+G V   + VEV SIGKIT QL DM LHHQKLV  D+L +L
Sbjct: 133 KYMEEVQQWRRAFTKVGDLTGVVVTKDSVEVDSIGKITNQLLDMLLHHQKLVPWDELTKL 192

Query: 182 VDIERRLCKMDMLIDLKSNKVHFVGIIGMGGIGKTTIAEVFYDKVAYKFGKNRCFLRTYE 241
           VDIER+L KM+ L DL+ N V F+GIIGMGGIGKTTIAEVFYD+VA  FGKNRCFLR YE
Sbjct: 193 VDIERQLFKMEKLNDLEPNVVRFIGIIGMGGIGKTTIAEVFYDRVARFFGKNRCFLRIYE 252

Query: 242 H-TLVSLQQQLLSQLLQTKDIIINNENEGATMIGSYLKEKKVLIVLDGVKDKSQLEKLVG 301
           H TL+SLQQQLLSQLLQTKD+IINNENEGA MIGS LK+K+VLIVLDGVK+K+QLE+LVG
Sbjct: 253 HTTLLSLQQQLLSQLLQTKDLIINNENEGARMIGSRLKDKRVLIVLDGVKEKTQLEQLVG 312

Query: 302 SPNWFGQGSQIIITTRNRDVLRQPNYKDKMVEYNVEFLDYKSAMSLFCKHAFGCEFPNKN 361
           +PNWFG GS+IIITTRNRDVLRQPNYKDKMVEY+VEFLD KSAM+LFCKHAFGC FP+KN
Sbjct: 313 NPNWFGSGSKIIITTRNRDVLRQPNYKDKMVEYSVEFLDTKSAMTLFCKHAFGCGFPSKN 372

Query: 362 YEDFCKEIVERVKGHPQALIQIGLSLYDQDIDIWKEELKNLREDYNKHLFKTLKISFDDL 421
           +EDF KEIVERV+GHPQALIQIG SLYD+ I+IWKEELK+L EDYN  +FKTLKISFDDL
Sbjct: 373 FEDFSKEIVERVEGHPQALIQIGSSLYDKGIEIWKEELKSLEEDYNNRIFKTLKISFDDL 432

Query: 422 GKTSQEAFLDLACFFNGKKKEKVIEILKSFDYRPHSEIQLLQDRCLIEVRRDNSIFMPKC 481
           GKTSQE FLDLACFFN K KEKVIEILKSFDYRPHSEIQLLQDRCLIEVR DN+I MPKC
Sbjct: 433 GKTSQEVFLDLACFFNEKTKEKVIEILKSFDYRPHSEIQLLQDRCLIEVRSDNTILMPKC 492

Query: 482 IQAMGQEIEREADKRSRIWLPKDALDVFDEGHRVKEIKGIVLEGEEKQDEVELEGRAFKE 541
           IQ MGQ+IEREADKRSRIWLPKDA DVFDE HRVK+IKG+VL+ EEKQDE+ELEG+ F++
Sbjct: 493 IQTMGQQIEREADKRSRIWLPKDAQDVFDEPHRVKDIKGVVLKLEEKQDEIELEGKVFED 552

Query: 542 MTCLKILEMSNVQLRGDFTHLSKQLRLLNWHNYPSQCLPLRFESRYLFELLLPLSQTRQL 601
           M  LKILE+ NV++ GDFTHLSKQLRLLNWH+YPSQCLPL FESRYLF+LLLPLSQTRQL
Sbjct: 553 MRSLKILEIGNVEVSGDFTHLSKQLRLLNWHSYPSQCLPLSFESRYLFQLLLPLSQTRQL 612

Query: 602 WNGQ------KVINISDSKNLRETPNFTKVPNLESLVLSNCTRLWKIDSSISRLNRLTLL 661
           WNGQ      KVIN+S SKNLRETPNFTKVPNLESL LSNCTRLWKIDSSI RLNRLTLL
Sbjct: 613 WNGQKGFEKLKVINVSGSKNLRETPNFTKVPNLESLDLSNCTRLWKIDSSIIRLNRLTLL 672

Query: 662 DLTCCINLKSIPSIKSCKSLTTLNLAGSGL--IGLEHSQN----VFQLAKRRS 698
           D+TCCI LK++P  +SCKSL T+N  GSGL   GL++ QN    ++   KRRS
Sbjct: 673 DITCCITLKNLPFSRSCKSLITINYVGSGLEEKGLDNFQNDDSLLYFQPKRRS 725

BLAST of Lsi08G001330 vs. TAIR 10
Match: AT5G36930.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 347.8 bits (891), Expect = 2.1e-95
Identity = 242/699 (34.62%), Postives = 360/699 (51.50%), Query Frame = 0

Query: 2   NTRNTFTGYLYKELVELGISTFMDDKKLLIGDRLSKKLIRAIEKSGSFIVVLSKNYASSK 61
           + R  F  +LY  L   GISTFMDD +L  G+ +S +L+ AIE S   IVVL+K+YASS 
Sbjct: 24  DVRKNFLSHLYDSLRRCGISTFMDDVELQRGEYISPELLNAIETSKILIVVLTKDYASSA 83

Query: 62  WCLRELTKIIDCMAEQ-KHRVLPVFYHVSPRDVRRQSGCFEKSFHEHEKILLELDNMERD 121
           WCL EL  I+        H V P+F +V P D+R Q G + KSF +H          +  
Sbjct: 84  WCLDELVHIMKSHKNNPSHMVFPIFLYVDPSDIRWQQGSYAKSFSKH----------KNS 143

Query: 122 KYMKEVQQWRRALTKVGDLSG-EVANIVEVVSIGKITKQLFDMLHHQKLVASDKLNQLVD 181
             + +++ WR ALTKV ++SG ++ N  E   I  IT+++   L  Q L      +  V 
Sbjct: 144 HPLNKLKDWREALTKVANISGWDIKNRNEAECIADITREILKRLPCQYLHVP---SYAVG 203

Query: 182 IERRLCKMDMLIDLKSNKVHFVGIIGMGGIGKTTIAEVFYDKVAYKFGKNRCFLRTYE-- 241
           +  RL  +  L+ + S+ V  + I GMGGIGKTT+A+V +++ ++ F +   FL  +   
Sbjct: 204 LRSRLQHISSLLSIGSDGVRVIVIYGMGGIGKTTLAKVAFNEFSHLF-EGSSFLENFREY 263

Query: 242 ----HTLVSLQQQLLSQLLQTKDIIINNENEGATMIGSYLKEKKVLIVLDGVKDKSQLEK 301
                    LQ QLLS +L+  DI     +     +    + K+VL+V+D V D  QL  
Sbjct: 264 SKKPEGRTHLQHQLLSDILRRNDIEFKGLDHA---VKERFRSKRVLLVVDDVDDVHQLNS 323

Query: 302 LVGSPNWFGQGSQIIITTRNRDVLRQPNYKDKMVEYNVEFLDYKSAMSLFCKHAFGCEFP 361
                + FG GS+IIITTRN  +L+Q   +     Y+ + LD   ++ LF  HAF    P
Sbjct: 324 AAIDRDCFGHGSRIIITTRNMHLLKQLRAEG---SYSPKELDGDESLELFSWHAFRTSEP 383

Query: 362 NKNYEDFCKEIVERVKGHPQALIQIGLSLYDQDIDIWKEELKNLREDYNKHLFKTLKISF 421
            K +    +E+V    G P A+  +G  L ++ I  W+  LK L+   N ++   L+ISF
Sbjct: 384 PKEFLQHSEEVVTYCAGLPLAVEVLGAFLIERSIREWESTLKLLKRIPNDNIQAKLQISF 443

Query: 422 DDLGKTSQEAFLDLACFFNGKKKEKVIEILKSFDYRPHSEIQLLQDRCLIEVRRDNSIFM 481
           + L    ++ FLD+ACFF G     V  IL   +  P   + LL +RCLI +   N+I M
Sbjct: 444 NALTIEQKDVFLDIACFFIGVDSYYVACILDGCNLYPDIVLSLLMERCLITI-SGNNIMM 503

Query: 482 PKCIQAMGQEIERE-----ADKRSRIWLPKDALDVFDEGHRVKEIKGIVLEGEEKQDEVE 541
              ++ MG++I RE       +RSR+W   D + V  +      I+G+ L+  +  D   
Sbjct: 504 HDLLRDMGRQIVREISPKKCGERSRLWSHNDVVGVLKKKSGTNAIEGLSLKA-DVMDFQY 563

Query: 542 LEGRAFKEMTCLKILEMSNVQLRGDFTHLSKQLRLLNWHNYPSQCLPLRFESRYLFELLL 601
            E  AF +M  L++LE+  V L G + H  K LR L WH +  +C P+      L  L L
Sbjct: 564 FEVEAFAKMQELRLLELRYVDLNGSYEHFPKDLRWLCWHGFSLECFPINLSLESLAALDL 623

Query: 602 PLSQTRQLWNGQ---------KVINISDSKNLRETPNFTKVPNLESLVLSNCTRLWKIDS 661
             S  ++ W  Q         K +++S S  LRETP+F+  PN+E L+L NC  L  +  
Sbjct: 624 QYSNLKRFWKAQSPPQPANMVKYLDLSHSVYLRETPDFSYFPNVEKLILINCKSLVLVHK 683

Query: 662 SISRLN-RLTLLDLTCCINLKSIP-SIKSCKSLTTLNLA 677
           SI  L+ +L LL+L+ CI L  +P  I   KSL +L L+
Sbjct: 684 SIGILDKKLVLLNLSSCIELDVLPEEIYKLKSLESLFLS 700

BLAST of Lsi08G001330 vs. TAIR 10
Match: AT5G36930.2 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 347.8 bits (891), Expect = 2.1e-95
Identity = 242/699 (34.62%), Postives = 360/699 (51.50%), Query Frame = 0

Query: 2   NTRNTFTGYLYKELVELGISTFMDDKKLLIGDRLSKKLIRAIEKSGSFIVVLSKNYASSK 61
           + R  F  +LY  L   GISTFMDD +L  G+ +S +L+ AIE S   IVVL+K+YASS 
Sbjct: 27  DVRKNFLSHLYDSLRRCGISTFMDDVELQRGEYISPELLNAIETSKILIVVLTKDYASSA 86

Query: 62  WCLRELTKIIDCMAEQ-KHRVLPVFYHVSPRDVRRQSGCFEKSFHEHEKILLELDNMERD 121
           WCL EL  I+        H V P+F +V P D+R Q G + KSF +H          +  
Sbjct: 87  WCLDELVHIMKSHKNNPSHMVFPIFLYVDPSDIRWQQGSYAKSFSKH----------KNS 146

Query: 122 KYMKEVQQWRRALTKVGDLSG-EVANIVEVVSIGKITKQLFDMLHHQKLVASDKLNQLVD 181
             + +++ WR ALTKV ++SG ++ N  E   I  IT+++   L  Q L      +  V 
Sbjct: 147 HPLNKLKDWREALTKVANISGWDIKNRNEAECIADITREILKRLPCQYLHVP---SYAVG 206

Query: 182 IERRLCKMDMLIDLKSNKVHFVGIIGMGGIGKTTIAEVFYDKVAYKFGKNRCFLRTYE-- 241
           +  RL  +  L+ + S+ V  + I GMGGIGKTT+A+V +++ ++ F +   FL  +   
Sbjct: 207 LRSRLQHISSLLSIGSDGVRVIVIYGMGGIGKTTLAKVAFNEFSHLF-EGSSFLENFREY 266

Query: 242 ----HTLVSLQQQLLSQLLQTKDIIINNENEGATMIGSYLKEKKVLIVLDGVKDKSQLEK 301
                    LQ QLLS +L+  DI     +     +    + K+VL+V+D V D  QL  
Sbjct: 267 SKKPEGRTHLQHQLLSDILRRNDIEFKGLDHA---VKERFRSKRVLLVVDDVDDVHQLNS 326

Query: 302 LVGSPNWFGQGSQIIITTRNRDVLRQPNYKDKMVEYNVEFLDYKSAMSLFCKHAFGCEFP 361
                + FG GS+IIITTRN  +L+Q   +     Y+ + LD   ++ LF  HAF    P
Sbjct: 327 AAIDRDCFGHGSRIIITTRNMHLLKQLRAEG---SYSPKELDGDESLELFSWHAFRTSEP 386

Query: 362 NKNYEDFCKEIVERVKGHPQALIQIGLSLYDQDIDIWKEELKNLREDYNKHLFKTLKISF 421
            K +    +E+V    G P A+  +G  L ++ I  W+  LK L+   N ++   L+ISF
Sbjct: 387 PKEFLQHSEEVVTYCAGLPLAVEVLGAFLIERSIREWESTLKLLKRIPNDNIQAKLQISF 446

Query: 422 DDLGKTSQEAFLDLACFFNGKKKEKVIEILKSFDYRPHSEIQLLQDRCLIEVRRDNSIFM 481
           + L    ++ FLD+ACFF G     V  IL   +  P   + LL +RCLI +   N+I M
Sbjct: 447 NALTIEQKDVFLDIACFFIGVDSYYVACILDGCNLYPDIVLSLLMERCLITI-SGNNIMM 506

Query: 482 PKCIQAMGQEIERE-----ADKRSRIWLPKDALDVFDEGHRVKEIKGIVLEGEEKQDEVE 541
              ++ MG++I RE       +RSR+W   D + V  +      I+G+ L+  +  D   
Sbjct: 507 HDLLRDMGRQIVREISPKKCGERSRLWSHNDVVGVLKKKSGTNAIEGLSLKA-DVMDFQY 566

Query: 542 LEGRAFKEMTCLKILEMSNVQLRGDFTHLSKQLRLLNWHNYPSQCLPLRFESRYLFELLL 601
            E  AF +M  L++LE+  V L G + H  K LR L WH +  +C P+      L  L L
Sbjct: 567 FEVEAFAKMQELRLLELRYVDLNGSYEHFPKDLRWLCWHGFSLECFPINLSLESLAALDL 626

Query: 602 PLSQTRQLWNGQ---------KVINISDSKNLRETPNFTKVPNLESLVLSNCTRLWKIDS 661
             S  ++ W  Q         K +++S S  LRETP+F+  PN+E L+L NC  L  +  
Sbjct: 627 QYSNLKRFWKAQSPPQPANMVKYLDLSHSVYLRETPDFSYFPNVEKLILINCKSLVLVHK 686

Query: 662 SISRLN-RLTLLDLTCCINLKSIP-SIKSCKSLTTLNLA 677
           SI  L+ +L LL+L+ CI L  +P  I   KSL +L L+
Sbjct: 687 SIGILDKKLVLLNLSSCIELDVLPEEIYKLKSLESLFLS 703

BLAST of Lsi08G001330 vs. TAIR 10
Match: AT1G72840.1 (Disease resistance protein (TIR-NBS-LRR class) )

HSP 1 Score: 347.1 bits (889), Expect = 3.6e-95
Identity = 254/746 (34.05%), Postives = 384/746 (51.47%), Query Frame = 0

Query: 1   MNTRNTFTGYLYKELVELGISTFMDDKKLLIGDRLSKKLIRAIEKSGSFIVVLSKNYASS 60
           ++TR T   +LY  L   G+ TF DD+KL IGD ++  L++AI+ S   +V+LS+NYA+S
Sbjct: 24  VDTRQTIVSHLYVALRNNGVLTFKDDRKLEIGDTIADGLVKAIQTSWFAVVILSENYATS 83

Query: 61  KWCLRELTKIIDCMAEQKHRVLPVFYHVSPRDVRRQSGCFEKSFHEHEKILLELDNMERD 120
            WCL EL  I+   +E++ +VLP+FY V P DVR Q G F  +F  +          E D
Sbjct: 84  TWCLEELRLIMQLHSEEQIKVLPIFYGVKPSDVRYQEGSFATAFQRY----------EAD 143

Query: 121 KYMKE-VQQWRRALTKVGDLSGEVAN--IVEVVSIGKITKQLFDMLHHQKLVASDKLNQL 180
             M+E V +WRRALT+V +LSG+ +   + E   I ++   +   L   K   S  L  L
Sbjct: 144 PEMEEKVSKWRRALTQVANLSGKHSRNCVDEADMIAEVVGGISSRLPRMK---STDLINL 203

Query: 181 VDIERRLCKMDMLIDLK-SNKVHFVGIIGMGGIGKTTIAEVFYDKVAYKFGKNRCFLRTY 240
           V +E  + KM +L+++   ++VH +GI GMGGIGK+TIA+  YD+ + +F  + CFL   
Sbjct: 204 VGMEAHMMKMTLLLNIGCEDEVHMIGIWGMGGIGKSTIAKCLYDRFSRQFPAH-CFLENV 263

Query: 241 E--HTLVSLQQQLLSQLLQTKDIIINNENEGATMIGSYLKEKKVLIVLDGVKDKSQLEKL 300
              + +  LQ++LLS +L  +D+ + +   G+  I   L  +KV +VLD V    QL  L
Sbjct: 264 SKGYDIKHLQKELLSHILYDEDVELWSMEAGSQEIKERLGHQKVFVVLDNVDKVEQLHGL 323

Query: 301 VGSPNWFGQGSQIIITTRNRDVLRQPNYKDKMVEYNVEFLDYKSAMSLFCKHAFGCEFPN 360
              P+WFG GS+IIITTR++ +L      +    Y V+ LD K A+ +F K AFG   P+
Sbjct: 324 AKDPSWFGPGSRIIITTRDKGLLNSCGVNN---IYEVKCLDDKDALQVFKKLAFGGRPPS 383

Query: 361 KNYEDFCKEIVERVKGHPQALIQIGLSLYD-QDIDIWKEELKNLREDYNKHLFKTLKISF 420
             +E           G P AL+     L     ID W++EL  L     K++ + L+ S+
Sbjct: 384 DGFEQLFIRASRLAHGLPSALVAFASHLSAIVAIDEWEDELALLETFPQKNVQEILRASY 443

Query: 421 DDLGKTSQEAFLDLACFFNGKKKEKVIEILKSFDYRPHSEIQLLQDRCLIEVRRDNSIFM 480
           D L +  +  FL +ACFFNG     +   LK+ D R    I  L  +CL+ +  D  I M
Sbjct: 444 DGLDQYDKTVFLHVACFFNGGHLRYIRAFLKNCDAR----INHLAAKCLVNISIDGCISM 503

Query: 481 PKCIQAMGQEIERE-----ADKRSRIWLPKDALDVFDEGHRVKEIKGIVLEGEEKQDEVE 540
              +   G+EI R+       K+  +W P +   V D     + ++G+ L   E  D + 
Sbjct: 504 HILLVQTGREIVRQESDWRPSKQRFLWDPTEIHYVLDSNTGTRRVEGLSLHLCEMADTLL 563

Query: 541 LEGRAFKEM---TCLKILE-----MSNVQLRGDFTHLSKQLRLLNWHNYPSQCLPLRFES 600
           L    F  M   T LK  +     +SN+QL  D   LS+ L+LL+W  YP   LP  F  
Sbjct: 564 LRNSVFGPMHNLTFLKFFQHLGGNVSNLQLISDDYVLSRNLKLLHWDAYPLTILPPIFRP 623

Query: 601 RYLFELLLPLSQTRQLWNGQK------VINISDSKNLRETPNFTKVPNLESLVLSNCTRL 660
             + EL L  S+   LW+G K      +++++ S+NLRE P  +   NLE L+L +CT L
Sbjct: 624 HTIIELSLRYSKLNSLWDGTKLLPNLRILDVTGSRNLRELPELSTAVNLEELILESCTSL 683

Query: 661 WKIDSSISRLNRLTLLDLTCCINLKSIPSIKSCKSLTTLNLAGSGLIGLEHSQNVFQLAK 720
            +I  SI+RL  L  L++  C  L+ +  +     L   +L+  GL              
Sbjct: 684 VQIPESINRL-YLRKLNMMYCDGLEGVILV---NDLQEASLSRWGL-------------- 724

BLAST of Lsi08G001330 vs. TAIR 10
Match: AT1G72840.2 (Disease resistance protein (TIR-NBS-LRR class) )

HSP 1 Score: 347.1 bits (889), Expect = 3.6e-95
Identity = 254/746 (34.05%), Postives = 384/746 (51.47%), Query Frame = 0

Query: 1   MNTRNTFTGYLYKELVELGISTFMDDKKLLIGDRLSKKLIRAIEKSGSFIVVLSKNYASS 60
           ++TR T   +LY  L   G+ TF DD+KL IGD ++  L++AI+ S   +V+LS+NYA+S
Sbjct: 24  VDTRQTIVSHLYVALRNNGVLTFKDDRKLEIGDTIADGLVKAIQTSWFAVVILSENYATS 83

Query: 61  KWCLRELTKIIDCMAEQKHRVLPVFYHVSPRDVRRQSGCFEKSFHEHEKILLELDNMERD 120
            WCL EL  I+   +E++ +VLP+FY V P DVR Q G F  +F  +          E D
Sbjct: 84  TWCLEELRLIMQLHSEEQIKVLPIFYGVKPSDVRYQEGSFATAFQRY----------EAD 143

Query: 121 KYMKE-VQQWRRALTKVGDLSGEVAN--IVEVVSIGKITKQLFDMLHHQKLVASDKLNQL 180
             M+E V +WRRALT+V +LSG+ +   + E   I ++   +   L   K   S  L  L
Sbjct: 144 PEMEEKVSKWRRALTQVANLSGKHSRNCVDEADMIAEVVGGISSRLPRMK---STDLINL 203

Query: 181 VDIERRLCKMDMLIDLK-SNKVHFVGIIGMGGIGKTTIAEVFYDKVAYKFGKNRCFLRTY 240
           V +E  + KM +L+++   ++VH +GI GMGGIGK+TIA+  YD+ + +F  + CFL   
Sbjct: 204 VGMEAHMMKMTLLLNIGCEDEVHMIGIWGMGGIGKSTIAKCLYDRFSRQFPAH-CFLENV 263

Query: 241 E--HTLVSLQQQLLSQLLQTKDIIINNENEGATMIGSYLKEKKVLIVLDGVKDKSQLEKL 300
              + +  LQ++LLS +L  +D+ + +   G+  I   L  +KV +VLD V    QL  L
Sbjct: 264 SKGYDIKHLQKELLSHILYDEDVELWSMEAGSQEIKERLGHQKVFVVLDNVDKVEQLHGL 323

Query: 301 VGSPNWFGQGSQIIITTRNRDVLRQPNYKDKMVEYNVEFLDYKSAMSLFCKHAFGCEFPN 360
              P+WFG GS+IIITTR++ +L      +    Y V+ LD K A+ +F K AFG   P+
Sbjct: 324 AKDPSWFGPGSRIIITTRDKGLLNSCGVNN---IYEVKCLDDKDALQVFKKLAFGGRPPS 383

Query: 361 KNYEDFCKEIVERVKGHPQALIQIGLSLYD-QDIDIWKEELKNLREDYNKHLFKTLKISF 420
             +E           G P AL+     L     ID W++EL  L     K++ + L+ S+
Sbjct: 384 DGFEQLFIRASRLAHGLPSALVAFASHLSAIVAIDEWEDELALLETFPQKNVQEILRASY 443

Query: 421 DDLGKTSQEAFLDLACFFNGKKKEKVIEILKSFDYRPHSEIQLLQDRCLIEVRRDNSIFM 480
           D L +  +  FL +ACFFNG     +   LK+ D R    I  L  +CL+ +  D  I M
Sbjct: 444 DGLDQYDKTVFLHVACFFNGGHLRYIRAFLKNCDAR----INHLAAKCLVNISIDGCISM 503

Query: 481 PKCIQAMGQEIERE-----ADKRSRIWLPKDALDVFDEGHRVKEIKGIVLEGEEKQDEVE 540
              +   G+EI R+       K+  +W P +   V D     + ++G+ L   E  D + 
Sbjct: 504 HILLVQTGREIVRQESDWRPSKQRFLWDPTEIHYVLDSNTGTRRVEGLSLHLCEMADTLL 563

Query: 541 LEGRAFKEM---TCLKILE-----MSNVQLRGDFTHLSKQLRLLNWHNYPSQCLPLRFES 600
           L    F  M   T LK  +     +SN+QL  D   LS+ L+LL+W  YP   LP  F  
Sbjct: 564 LRNSVFGPMHNLTFLKFFQHLGGNVSNLQLISDDYVLSRNLKLLHWDAYPLTILPPIFRP 623

Query: 601 RYLFELLLPLSQTRQLWNGQK------VINISDSKNLRETPNFTKVPNLESLVLSNCTRL 660
             + EL L  S+   LW+G K      +++++ S+NLRE P  +   NLE L+L +CT L
Sbjct: 624 HTIIELSLRYSKLNSLWDGTKLLPNLRILDVTGSRNLRELPELSTAVNLEELILESCTSL 683

Query: 661 WKIDSSISRLNRLTLLDLTCCINLKSIPSIKSCKSLTTLNLAGSGLIGLEHSQNVFQLAK 720
            +I  SI+RL  L  L++  C  L+ +  +     L   +L+  GL              
Sbjct: 684 VQIPESINRL-YLRKLNMMYCDGLEGVILV---NDLQEASLSRWGL-------------- 724

BLAST of Lsi08G001330 vs. TAIR 10
Match: AT4G12010.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 336.3 bits (861), Expect = 6.4e-92
Identity = 233/718 (32.45%), Postives = 358/718 (49.86%), Query Frame = 0

Query: 2   NTRNTFTGYLYKELVELGISTFMDDKKLLIGDRLSKKLIRAIEKSGSFIVVLSKNYASSK 61
           +TRN FTG+L K L   GI +F+DD +L  GD L+  L   IEKS   I+V S NYA+S 
Sbjct: 21  DTRNNFTGHLQKALRLRGIDSFIDD-RLRRGDNLT-ALFDRIEKSKIAIIVFSTNYANSA 80

Query: 62  WCLRELTKIIDCMAEQKHRVLPVFYHVSPRDVRRQSGCFEKSFHEHEKILLELDNMERDK 121
           WCLREL KI++C    +  V+P+FY V   DV +Q   F   F   E     +       
Sbjct: 81  WCLRELVKILECRNSNQQLVVPIFYKVDKSDVEKQRNSFAVPFKLPELTFPGVT------ 140

Query: 122 YMKEVQQWRRALTKVGDLSGEVANIVEVVSIGKITKQLFDMLHHQKLVASDKLNQLVDIE 181
             +E+  W+ AL    ++ G V   +       + +   D       +A      LV IE
Sbjct: 141 -PEEISSWKAALASASNILGYVVKEISTSEAKLVDEIAVDTFKKLNDLAPSGNEGLVGIE 200

Query: 182 RRLCKMDMLIDLKS-NKVHFVGIIGMGGIGKTTIAEVFYDKVAYKFGKNRCFLRTYEHT- 241
            RL  ++ L+  +  + VH +GI+GM GIGKTT+A+  Y ++  +F    CFL       
Sbjct: 201 SRLKNLEKLLSWEDLDTVHIIGIVGMVGIGKTTLADCLYGRMRGQF-DGSCFLTNIRENS 260

Query: 242 ----LVSLQQQLLSQLLQTKDIIINNENEGATMIGSYLKEKKVLIVLDGVKDKSQLEKLV 301
               L SL Q+L S +L  +D+ I             LK K++LIVLD V D+ Q+  L+
Sbjct: 261 GRSGLESLLQKLFSTVLNDRDLEIGAPGNAHERFERRLKSKRLLIVLDDVNDEKQIRYLM 320

Query: 302 GSPNWFGQGSQIIITTRNRDVLRQPNYKDKMVEYNVEFLDYKSAMSLFCKHAFGCEFPNK 361
           G   W+  GS+IIITTR+  ++       K  +Y +  L+ + A+ LF  +AF   FP K
Sbjct: 321 GHCKWYQGGSRIIITTRDSKLIE----TIKGRKYVLPKLNDREALKLFSLNAFSNSFPLK 380

Query: 362 NYEDFCKEIVERVKGHPQALIQIGLSLYDQDIDIWKEELKNLREDYNKHLFKTLKISFDD 421
            +E     +++  KGHP AL  +G  L ++D   W+ +L  L+   +  +++ L+ S+++
Sbjct: 381 EFEGLTNMVLDYAKGHPLALKVLGSDLCERDDLYWEAKLDRLKSRSHGDIYEVLETSYEE 440

Query: 422 LGKTSQEAFLDLACFFNGKKKEKVIEILKSFDYRPHSEIQLLQDRCLIEVRRDNSIFMPK 481
           L    +  FLD+ACFF  +  + V  +L S        ++ L D+CLI +  DN I M  
Sbjct: 441 LTTEQKNVFLDIACFFRSENVDYVTSLLNSHGVDVSGVVKDLVDKCLITL-SDNRIEMHD 500

Query: 482 CIQAMGQEIEREADK------------------RSRIWLPKDALDVFDEGHRVKEIKGIV 541
            +Q M +EI  + +                     R+W  +D  D+  EG    +I+GI 
Sbjct: 501 MLQTMAKEISLKVETIGIRDCRWLSRHGNQCQWHIRLWDSEDICDLLTEGLGTDKIRGIF 560

Query: 542 LEGEEKQDEVELEGRAFKEMTCLKILEMSN------------VQLRGDFTHLSKQLRLLN 601
           L+   K   + L  +AF+ M  LK L++ +            + LR   + L  +L  L+
Sbjct: 561 LD-TSKLRAMRLSAKAFQGMYNLKYLKIYDSHCSRGCEAEFKLHLRRGLSFLPNELTYLH 620

Query: 602 WHNYPSQCLPLRFESRYLFELLLPLSQTRQLWNGQ------KVINISDSKNLRETPNFTK 661
           WH YP Q +PL F+ + L +L LP SQ  ++W+ +      K +++S S NLR+      
Sbjct: 621 WHGYPLQSIPLDFDPKNLVDLKLPHSQLEEIWDDEKDVGMLKWVDLSHSINLRQCLGLAN 680

Query: 662 VPNLESLVLSNCTRLWKIDSSISRLNRLTLLDLTCCINLKSIPSIKSCKSLTTLNLAG 678
             NLE L L  CT L K+ S+I+ L +L  L+L  C +L+S+P     +SL TL L+G
Sbjct: 681 AHNLERLNLEGCTSLKKLPSTINCLEKLIYLNLRDCTSLRSLPKGIKTQSLQTLILSG 722