Lsi08G000460 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi08G000460
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionPlant protein of unknown function (DUF247)
Locationchr08: 1105174 .. 1106790 (-)
RNA-Seq ExpressionLsi08G000460
SyntenyLsi08G000460
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCCGGATTCTCCATTCTCAACGGAACACTCGACATATCTCAGTGGGTCCACCAAATCAACGACCTTATTGACCAAAATGCCCTCCCCGCCGCCTCTCAGACCCCTATTTCCGTCTTCCGGCTGCCGGAATCCGTCCACGCCGGCGACCCGACGGCGTACCTCCCCCACCATGTGGCGTTGGGGCCGTACCACCATTTCCGGCCAGAGCTTTATAAGATGGACTTGTTCAAACTTGGGAAAGCCAAGAATCTGAAATGGGGTCCCCAAATCCATAAGCTTGCAGATCAGCTTAAGCCATTGGAGCTCAAAATCCGAGCCTGTTTCGATCAAGGCCATGAAATGAATGGCGAAACCTTATCATGGGTAGTCCTGATTGATGGTTTGTTTCTACTTGATCTACTTCAAAAGAAGTTCTTGAATCGTCCCCTTAAGTTCCCTTTAGACATATTTTATGGGAAGTTATGGTCTGAGTTTGAGATTGTTAGTGATATAGTGAAACTTGAGAATCAGATTCCATTGTTTGTTCTGAATGAAATTTGCCCCAAAGAACCCATCAACTACGTGGCTCCTTTTCTATACCAATTTTCTGCATCTGTTTCACCATTTCAATTGCCCTGTTTTAACCCTGAATTGGAATGTTTTGGATTCTCCTCTTATTTGCCTGAAATCTTCAATTTGTCTCACCATTTGCTACATTTTCTCTATTCTCTGGTTTTGGTTATTCCCGTTGAGGTACCCATATCTAATCGTAATCGTACGCGAATGAATTGGGAGAATTCTTCTTCTTCTTCTTCTTCTGTTGAATTTCTGATCGAGTGCTTCAACATTTTGGGCTCTGTTATTAACATAGCCTTCTTTCAACAATTGAAAGATACCTTTGGTTTAATTCAACGCTTATTTAGACTTTTGGGTCTTATAACTAAAACAAATTCGGCTGAGAGAAAAACCCCTTTAATTATCCCTTCTGCATCAGACTTGAAAACTGCTGGAGTTACTTTTAAATCCACCAAAACTGGCATTCTCAAGTGTCATTTTGATAAGACAACGCTCACTTTAACATTGCCTTCTATTCATCTTGATGGGTTCTCTCAAGTTTTGCTCAAGAATCTAGTGGCATTTGAAGCAATGGCAGAGCTGAATCCGCCTTGTTTTGTCAATTACACTGCACTGATGAATGGGTTATTGAGAAATTCGAAGGACTTGAAGATTTTGGAGAAAGCACAGATTGTTCATAACCATTTGGACAGTGAAGAAGAGGCTGTGGAGCTGTTTAATGGGGTTGAGAACTCAACCAGCAGCTTGAAGAAAGAGAGAGTTCTGAAAATTCATCTCAATAGTGGTCTGAATTCTGGGAATAGAAGTATGTTCATTCGAGATGTCAAGATCAGCTATGAAATGGGATTGGGGGCTATGGCTGATGAGATCAACAAATTCTATGCTAATTGTTGGAGAATGAAGATGAAGAAATTTATGTCTGTTGTATTCAAGTGTTTTACAATTCTTGTCATTGTCTTCGTCATTGTTCTTGTGATTGTTCGATTTGTTTGCAATTTTCTCACCTGTCCTGTGGGAATCTTTAAAACTAGCATTTCTCTTCATCAGATGTATTGA

mRNA sequence

ATGGCTTCCGGATTCTCCATTCTCAACGGAACACTCGACATATCTCAGTGGGTCCACCAAATCAACGACCTTATTGACCAAAATGCCCTCCCCGCCGCCTCTCAGACCCCTATTTCCGTCTTCCGGCTGCCGGAATCCGTCCACGCCGGCGACCCGACGGCGTACCTCCCCCACCATGTGGCGTTGGGGCCGTACCACCATTTCCGGCCAGAGCTTTATAAGATGGACTTGTTCAAACTTGGGAAAGCCAAGAATCTGAAATGGGGTCCCCAAATCCATAAGCTTGCAGATCAGCTTAAGCCATTGGAGCTCAAAATCCGAGCCTGTTTCGATCAAGGCCATGAAATGAATGGCGAAACCTTATCATGGGTAGTCCTGATTGATGGTTTGTTTCTACTTGATCTACTTCAAAAGAAGTTCTTGAATCGTCCCCTTAAGTTCCCTTTAGACATATTTTATGGGAAGTTATGGTCTGAGTTTGAGATTGTTAGTGATATAGTGAAACTTGAGAATCAGATTCCATTGTTTGTTCTGAATGAAATTTGCCCCAAAGAACCCATCAACTACGTGGCTCCTTTTCTATACCAATTTTCTGCATCTGTTTCACCATTTCAATTGCCCTGTTTTAACCCTGAATTGGAATGTTTTGGATTCTCCTCTTATTTGCCTGAAATCTTCAATTTGTCTCACCATTTGCTACATTTTCTCTATTCTCTGGTTTTGGTTATTCCCGTTGAGGTACCCATATCTAATCGTAATCGTACGCGAATGAATTGGGAGAATTCTTCTTCTTCTTCTTCTTCTGTTGAATTTCTGATCGAGTGCTTCAACATTTTGGGCTCTGTTATTAACATAGCCTTCTTTCAACAATTGAAAGATACCTTTGGTTTAATTCAACGCTTATTTAGACTTTTGGGTCTTATAACTAAAACAAATTCGGCTGAGAGAAAAACCCCTTTAATTATCCCTTCTGCATCAGACTTGAAAACTGCTGGAGTTACTTTTAAATCCACCAAAACTGGCATTCTCAAGTGTCATTTTGATAAGACAACGCTCACTTTAACATTGCCTTCTATTCATCTTGATGGGTTCTCTCAAGTTTTGCTCAAGAATCTAGTGGCATTTGAAGCAATGGCAGAGCTGAATCCGCCTTGTTTTGTCAATTACACTGCACTGATGAATGGGTTATTGAGAAATTCGAAGGACTTGAAGATTTTGGAGAAAGCACAGATTGTTCATAACCATTTGGACAGTGAAGAAGAGGCTGTGGAGCTGTTTAATGGGGTTGAGAACTCAACCAGCAGCTTGAAGAAAGAGAGAGTTCTGAAAATTCATCTCAATAGTGGTCTGAATTCTGGGAATAGAAGTATGTTCATTCGAGATGTCAAGATCAGCTATGAAATGGGATTGGGGGCTATGGCTGATGAGATCAACAAATTCTATGCTAATTGTTGGAGAATGAAGATGAAGAAATTTATGTCTGTTGTATTCAAGTGTTTTACAATTCTTGTCATTGTCTTCGTCATTGTTCTTGTGATTGTTCGATTTGTTTGCAATTTTCTCACCTGTCCTGTGGGAATCTTTAAAACTAGCATTTCTCTTCATCAGATGTATTGA

Coding sequence (CDS)

ATGGCTTCCGGATTCTCCATTCTCAACGGAACACTCGACATATCTCAGTGGGTCCACCAAATCAACGACCTTATTGACCAAAATGCCCTCCCCGCCGCCTCTCAGACCCCTATTTCCGTCTTCCGGCTGCCGGAATCCGTCCACGCCGGCGACCCGACGGCGTACCTCCCCCACCATGTGGCGTTGGGGCCGTACCACCATTTCCGGCCAGAGCTTTATAAGATGGACTTGTTCAAACTTGGGAAAGCCAAGAATCTGAAATGGGGTCCCCAAATCCATAAGCTTGCAGATCAGCTTAAGCCATTGGAGCTCAAAATCCGAGCCTGTTTCGATCAAGGCCATGAAATGAATGGCGAAACCTTATCATGGGTAGTCCTGATTGATGGTTTGTTTCTACTTGATCTACTTCAAAAGAAGTTCTTGAATCGTCCCCTTAAGTTCCCTTTAGACATATTTTATGGGAAGTTATGGTCTGAGTTTGAGATTGTTAGTGATATAGTGAAACTTGAGAATCAGATTCCATTGTTTGTTCTGAATGAAATTTGCCCCAAAGAACCCATCAACTACGTGGCTCCTTTTCTATACCAATTTTCTGCATCTGTTTCACCATTTCAATTGCCCTGTTTTAACCCTGAATTGGAATGTTTTGGATTCTCCTCTTATTTGCCTGAAATCTTCAATTTGTCTCACCATTTGCTACATTTTCTCTATTCTCTGGTTTTGGTTATTCCCGTTGAGGTACCCATATCTAATCGTAATCGTACGCGAATGAATTGGGAGAATTCTTCTTCTTCTTCTTCTTCTGTTGAATTTCTGATCGAGTGCTTCAACATTTTGGGCTCTGTTATTAACATAGCCTTCTTTCAACAATTGAAAGATACCTTTGGTTTAATTCAACGCTTATTTAGACTTTTGGGTCTTATAACTAAAACAAATTCGGCTGAGAGAAAAACCCCTTTAATTATCCCTTCTGCATCAGACTTGAAAACTGCTGGAGTTACTTTTAAATCCACCAAAACTGGCATTCTCAAGTGTCATTTTGATAAGACAACGCTCACTTTAACATTGCCTTCTATTCATCTTGATGGGTTCTCTCAAGTTTTGCTCAAGAATCTAGTGGCATTTGAAGCAATGGCAGAGCTGAATCCGCCTTGTTTTGTCAATTACACTGCACTGATGAATGGGTTATTGAGAAATTCGAAGGACTTGAAGATTTTGGAGAAAGCACAGATTGTTCATAACCATTTGGACAGTGAAGAAGAGGCTGTGGAGCTGTTTAATGGGGTTGAGAACTCAACCAGCAGCTTGAAGAAAGAGAGAGTTCTGAAAATTCATCTCAATAGTGGTCTGAATTCTGGGAATAGAAGTATGTTCATTCGAGATGTCAAGATCAGCTATGAAATGGGATTGGGGGCTATGGCTGATGAGATCAACAAATTCTATGCTAATTGTTGGAGAATGAAGATGAAGAAATTTATGTCTGTTGTATTCAAGTGTTTTACAATTCTTGTCATTGTCTTCGTCATTGTTCTTGTGATTGTTCGATTTGTTTGCAATTTTCTCACCTGTCCTGTGGGAATCTTTAAAACTAGCATTTCTCTTCATCAGATGTATTGA

Protein sequence

MASGFSILNGTLDISQWVHQINDLIDQNALPAASQTPISVFRLPESVHAGDPTAYLPHHVALGPYHHFRPELYKMDLFKLGKAKNLKWGPQIHKLADQLKPLELKIRACFDQGHEMNGETLSWVVLIDGLFLLDLLQKKFLNRPLKFPLDIFYGKLWSEFEIVSDIVKLENQIPLFVLNEICPKEPINYVAPFLYQFSASVSPFQLPCFNPELECFGFSSYLPEIFNLSHHLLHFLYSLVLVIPVEVPISNRNRTRMNWENSSSSSSSVEFLIECFNILGSVINIAFFQQLKDTFGLIQRLFRLLGLITKTNSAERKTPLIIPSASDLKTAGVTFKSTKTGILKCHFDKTTLTLTLPSIHLDGFSQVLLKNLVAFEAMAELNPPCFVNYTALMNGLLRNSKDLKILEKAQIVHNHLDSEEEAVELFNGVENSTSSLKKERVLKIHLNSGLNSGNRSMFIRDVKISYEMGLGAMADEINKFYANCWRMKMKKFMSVVFKCFTILVIVFVIVLVIVRFVCNFLTCPVGIFKTSISLHQMY
Homology
BLAST of Lsi08G000460 vs. ExPASy Swiss-Prot
Match: P0C897 (Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana OX=3702 GN=At3g02645 PE=3 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 2.9e-38
Identity = 139/540 (25.74%), Postives = 250/540 (46.30%), Query Frame = 0

Query: 13  DISQWVHQINDLIDQNALP-AASQTPISVFRLPESVHAGDPTAYLPHHVALGPYHHFRPE 72
           D ++WV  +   +D         +  +S+F +P+++    P +Y PH V++GPYH  +PE
Sbjct: 17  DETRWVINVQKSLDAELEEHDLEEVTVSIFNVPKALMCSHPDSYTPHRVSIGPYHCLKPE 76

Query: 73  LYKMDLFKL---GKAKNLKWGPQIHKLADQLKPLELKIRACFDQGHEMNGETLSWVVLID 132
           L++M+ +KL    K +N     + H L ++L+ +E+KIRAC+ +    NGETL W++ +D
Sbjct: 77  LHEMERYKLMIARKIRNQYNSFRFHDLVEKLQSMEIKIRACYHKYIGFNGETLLWIMAVD 136

Query: 133 GLFLLDLLQKKFLNRPLKFPLDIFYGKLWSEFEIVSDIVKLENQIPLFVLNEICPKEPIN 192
             FL++ L+     +     ++    ++    EI+ DI+ +ENQIPLFVL +        
Sbjct: 137 SSFLIEFLKIYSFRK-----VETLINRV-GHNEILRDIMMIENQIPLFVLRK-------- 196

Query: 193 YVAPFLYQFSASVSPFQLPC-------FNPELECFGFSSYLPEIFNLSHHLLHFLYSLVL 252
               F  + + S     L          +P +  F     L   F   +H+L FLY +++
Sbjct: 197 -TLEFQLESTESADDLLLSVLTGLCKDLSPLVIKFDDDQILKAQFQECNHILDFLYQMIV 256

Query: 253 VIPVEVPISNRNRTRMNWENSSSSSSSVEFLIECFNILGSVI-----------------N 312
               E  +   +    N  + +  + ++ F+ E  +    V                  N
Sbjct: 257 PRIEEEELEEDDEE--NRADENGGNRAIRFMDEIKHQFKRVFASRPADLILRFPWRIISN 316

Query: 313 IAFFQQLKDTFGLIQRLFRLLGLITKTNSAE----RKTPLI----IPSASDLKTAGVTFK 372
           +  F  LK +   +          T+  S       K PL+    IPS SDL  AGV FK
Sbjct: 317 LPGFMALKLSADYLFTRQENEATTTRQESVSILDIEKPPLVEELTIPSVSDLHKAGVRFK 376

Query: 373 STKTG-ILKCHFDKTTLTLTLPSIHLDGFSQVLLKNLVAFEAMAELNPPCFVNYTALMNG 432
            T  G I    FD  +    LP I+LD  ++ +L+NLVA+EA     P  F  YT L+NG
Sbjct: 377 PTAHGNISTVTFDSNSGQFYLPVINLDINTETVLRNLVAYEATNTSGPLVFTRYTELING 436

Query: 433 LLRNSKDLKILEKAQIVHNHLDSEEEAVELFNGVENSTSSLKKERVLKIHLNSGLNSGNR 492
           ++ + +D+++L +  ++ + L S++EA E++NG+  S                       
Sbjct: 437 IIDSEEDVRLLREQGVLVSRLKSDQEAAEMWNGMSKS----------------------- 496

Query: 493 SMFIRDVKISYEMGLGAMADEINKFYANCWRMKMKKFMSV-VFKCFTILVIVFVIVLVIV 515
              +R  K+ +   L    +++N++Y   W++K+ + + V V+  + IL  +  ++L+++
Sbjct: 497 ---VRLTKVGF---LDKTIEDVNRYYTGRWKVKIGRLVEVYVYGSWQILAFLAAVLLLML 510

BLAST of Lsi08G000460 vs. ExPASy Swiss-Prot
Match: Q9SD53 (UPF0481 protein At3g47200 OS=Arabidopsis thaliana OX=3702 GN=At3g47200 PE=2 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 2.5e-13
Identity = 102/410 (24.88%), Postives = 162/410 (39.51%), Query Frame = 0

Query: 30  LPAASQTPISVFRLPESVHAGDPTAYLPHHVALGPYHHFRPEL-----YKMDLFKLGKAK 89
           L +A +    +FR+PES  A +P AY P  V++GPYH+    L     +K  L +L   +
Sbjct: 38  LESAGKESCCIFRVPESFVALNPKAYKPKVVSIGPYHYGEKHLQMIQQHKPRLLQLFLDE 97

Query: 90  NLKWGPQIHKLADQLKPLELKIRACFDQGHEMNGETLSWVVLIDGLFLLDLLQKKFLNRP 149
             K   + + L   +  LE KIR  + +     G  L +++++DG F+L +     ++  
Sbjct: 98  AKKKDVEENVLVKAVVDLEDKIRKSYSE-ELKTGHDLMFMMVLDGCFILMVF--LIMSGN 157

Query: 150 LKFPLDIFYGKLWSEFEIVSDIVKLENQIPLFVLNEICPKEPINYVAPFLYQFSASVSPF 209
           ++   D  +   W    I SD++ LENQ+P FVL  +     I          S+ ++  
Sbjct: 158 IELSEDPIFSIPWLLSSIQSDLLLLENQVPFFVLQTLYVGSKIG--------VSSDLNRI 217

Query: 210 QLPCFNPELECFGFSSYLPEIFNL-SHHLLHFLYSLVLVIPVEVPISNRNRTRMNWENSS 269
               F   ++  G  SY  +  N  + HLL  +         E  + N +      E+  
Sbjct: 218 AFHFFKNPIDKEG--SYWEKHRNYKAKHLLDLIR--------ETFLPNTS------ESDK 277

Query: 270 SSSSSVEFLIECFNILGSVINIAFFQQLKDTFGLIQRLFRLLGLITKTNSAERKTPLIIP 329
           +SS  V+                   QL +            G      S + K   +I 
Sbjct: 278 ASSPHVQV------------------QLHE------------GKSGNVPSVDSKAVPLIL 337

Query: 330 SASDLKTAGVTF---KSTKTGILKCHFDKTTLTLTLPSIHLDGFSQVLLKNLVAFEAMAE 389
           SA  L+  G+ F   +S +  IL     K    L +P +  DGF      N VAFE    
Sbjct: 338 SAKRLRLQGIKFRLRRSKEDSILNVRLKKN--KLQIPQLRFDGFISSFFLNCVAFEQFYT 388

Query: 390 LNPPCFVNYTALMNGLLRNSKDLKILEKAQ-IVHNHLDSEEEAVELFNGV 430
            +      Y   M  LL N +D+  L   + I+ NH  S  E  E F  +
Sbjct: 398 DSSNEITTYIVFMGCLLNNEEDVTFLRNDKLIIENHFGSNNEVSEFFKTI 388

BLAST of Lsi08G000460 vs. ExPASy TrEMBL
Match: A0A0A0KSX9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G650580 PE=4 SV=1)

HSP 1 Score: 802.7 bits (2072), Expect = 9.1e-229
Identity = 409/538 (76.02%), Postives = 461/538 (85.69%), Query Frame = 0

Query: 1   MASGFSILNGTLDISQWVHQINDLIDQNALPAASQTPISVFRLPESVHAGDPTAYLPHHV 60
           MASGFSIL GT D SQW+HQIN+L++Q+AL AASQ PISVFRLPESVH  DPTAYLP HV
Sbjct: 1   MASGFSILTGTFDKSQWLHQINNLVNQDALLAASQIPISVFRLPESVHDVDPTAYLPRHV 60

Query: 61  ALGPYHHFRPELYKMDLFKLGKAKNLKWGPQIHKLADQLKPLELKIRACFDQGHEMNGET 120
           A GP+HHF+PELYKM+LFKLGKAKNLKWGPQIHKL+D+L PLELKIRACFDQ  E+NGET
Sbjct: 61  AFGPFHHFQPELYKMELFKLGKAKNLKWGPQIHKLSDRLTPLELKIRACFDQALEINGET 120

Query: 121 LSWVVLIDGLFLLDLLQKKFLNRPLKFPLDIFYGKLWSEFEIVSDIVKLENQIPLFVLNE 180
           LSW++LIDGLFL+ LLQ +++N PL FP +IFYGKLWSEFEIV D+VKLENQIPLFVLNE
Sbjct: 121 LSWLLLIDGLFLIYLLQNEYMNCPLTFPSEIFYGKLWSEFEIVCDMVKLENQIPLFVLNE 180

Query: 181 ICPKEPINYVAPFLYQFSASVSPFQLPCFNPELECFGFSSYLPEIFNLSHHLLHFLYSLV 240
           ICP+EPIN++AP LYQF  SVSPFQLPCFNP LECFGFSSYLPEIF+LSHHLLHFLYSL+
Sbjct: 181 ICPEEPINFLAPSLYQFCVSVSPFQLPCFNPRLECFGFSSYLPEIFDLSHHLLHFLYSLI 240

Query: 241 LVIPVEVPISNRNRTRMNWENSSSSSSSVEFLIECFNILGSVINIAFFQQLKDTFGLIQR 300
           L+IP E  +   +  RM  +NSSSSSSSVEFL EC +ILGSVINIAF QQ+K+T GLIQR
Sbjct: 241 LLIPNERVVFVAHCFRM--DNSSSSSSSVEFLSECLDILGSVINIAFIQQIKETIGLIQR 300

Query: 301 LFRLLGLITKTNSAERKTPLIIPSASDLKTAGVTFKSTKTGILKCHFDKTTLTLTLPSIH 360
           L RLL  ITK N AE+   LIIPSASDLK+AG TFKSTK GILK +FD+TTLTLTLP IH
Sbjct: 301 LLRLLSFITKPNLAEKTPLLIIPSASDLKSAGFTFKSTKNGILKSNFDETTLTLTLPCIH 360

Query: 361 LDGFSQVLLKNLVAFEAMAELNPPCFVNYTALMNGLLRNSKDLKILEKAQIVHNHLDSEE 420
           LDGF+ VLLKNLVAFEAMAELNPPC  NYTALMNGLLRNSKDLK+LEKA+IVHNHL+SEE
Sbjct: 361 LDGFTHVLLKNLVAFEAMAELNPPCLANYTALMNGLLRNSKDLKVLEKAEIVHNHLNSEE 420

Query: 421 EAVELFNGVENSTSSLKKERVLKIHLNSGLNSGNRSMFIRDVKISY-EMGLGAMADEINK 480
           EA ELF GVENSTS LKK  +LKIHLNSG N  N S FI++V+ SY EMGLG M + IN 
Sbjct: 421 EAAELFYGVENSTSKLKKGSLLKIHLNSGFNFENGSKFIQEVESSYIEMGLGDMVERINN 480

Query: 481 FYANCWRMKMKKFMSVVFKCFTILVIVFVIVLVIVRFVCNFLTCPVGIFKTSISLHQM 538
            Y NCWRMKMKKF+SVV+KCF +LV+VF+IVLV  RFVCNFL+CP G F  + +LHQM
Sbjct: 481 CYGNCWRMKMKKFVSVVYKCFAVLVVVFLIVLVTTRFVCNFLSCPFGTFMNTTALHQM 536

BLAST of Lsi08G000460 vs. ExPASy TrEMBL
Match: A0A5D3BMY1 (Putative UPF0481 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G00380 PE=4 SV=1)

HSP 1 Score: 792.7 bits (2046), Expect = 9.4e-226
Identity = 403/537 (75.05%), Postives = 455/537 (84.73%), Query Frame = 0

Query: 1   MASGFSILNGTLDISQWVHQINDLIDQNALPAASQTPISVFRLPESVHAGDPTAYLPHHV 60
           MASGFSIL GT D SQWV QINDL+DQNAL  A+QTPIS+FRLPESVH GDPTAYLP HV
Sbjct: 1   MASGFSILTGTFDKSQWVLQINDLVDQNALLDATQTPISIFRLPESVHDGDPTAYLPRHV 60

Query: 61  ALGPYHHFRPELYKMDLFKLGKAKNLKWGPQIHKLADQLKPLELKIRACFDQGHEMNGET 120
           A GP+HHF+PELYKM+LFKLGKAKNLKWGPQIHKLAD+L PLELK+RACFDQ  E+NGET
Sbjct: 61  AFGPFHHFQPELYKMELFKLGKAKNLKWGPQIHKLADRLTPLELKMRACFDQALEINGET 120

Query: 121 LSWVVLIDGLFLLDLLQKKFLNRPLKFPLDIFYGKLWSEFEIVSDIVKLENQIPLFVLNE 180
           LSWV+LIDGLFL+ LLQ K++NRPL+FP +IFYGKLWSEFEIV D+VKLENQIPLFVLNE
Sbjct: 121 LSWVLLIDGLFLIYLLQNKYMNRPLRFPSEIFYGKLWSEFEIVGDMVKLENQIPLFVLNE 180

Query: 181 ICPKEPINYVAPFLYQFSASVSPFQLPCFNPELECFGFSSYLPEIFNLSHHLLHFLYSLV 240
           ICPKEPIN++APFLYQF  SVS F LP FNP+LECFGFSSYLPEIF+LSHHLLHFLYSL+
Sbjct: 181 ICPKEPINFLAPFLYQFCVSVSSFPLPRFNPQLECFGFSSYLPEIFDLSHHLLHFLYSLI 240

Query: 241 LVIPVEVPISNRNRTRMNWENSSSSSSSVEFLIECFNILGSVINIAFFQQLKDTFGLIQR 300
           L+IP E         R++    +SSSSSVEFL E  +ILGSVINIAF QQ+K+T GLIQR
Sbjct: 241 LLIPDERVAFMPYCFRLD----NSSSSSVEFLSESLDILGSVINIAFIQQIKETIGLIQR 300

Query: 301 LFRLLGLITKTNSAERKTPLIIPSASDLKTAGVTFKSTKTGILKCHFDKTTLTLTLPSIH 360
           L RLL  ITK N AE+  PLIIPSASDLK+AG TFKSTK GILK +FD+TTLTLTLP IH
Sbjct: 301 LLRLLSSITKPNLAEKTPPLIIPSASDLKSAGFTFKSTKNGILKSYFDETTLTLTLPCIH 360

Query: 361 LDGFSQVLLKNLVAFEAMAELNPPCFVNYTALMNGLLRNSKDLKILEKAQIVHNHLDSEE 420
           L GF+ VLLKNLVAFE+MAELNPPC  NYTALMNGLLR SKDLK+LEKA+IVHNHL+SEE
Sbjct: 361 LHGFTHVLLKNLVAFESMAELNPPCLANYTALMNGLLRKSKDLKVLEKAEIVHNHLNSEE 420

Query: 421 EAVELFNGVENSTSSLKKERVLKIHLNSGLNSGNRSMFIRDVKISYEMGLGAMADEINKF 480
           EA ELF GVENSTS LKKE +L+IHL  G N  N S FI +V+ SYEMGLG M + IN  
Sbjct: 421 EAAELFYGVENSTSRLKKESLLEIHLKGGFNFENGSNFIPEVESSYEMGLGNMVERINNC 480

Query: 481 YANCWRMKMKKFMSVVFKCFTILVIVFVIVLVIVRFVCNFLTCPVGIFKTSISLHQM 538
           Y NCWRMKMKK +SVV+KCF +LV+VF+IVLV  RFVCNF++CP+G F T+ +LHQM
Sbjct: 481 YGNCWRMKMKKVVSVVYKCFAVLVVVFLIVLVTTRFVCNFMSCPLGTFMTTTALHQM 533

BLAST of Lsi08G000460 vs. ExPASy TrEMBL
Match: A0A1S3CAN6 (putative UPF0481 protein At3g02645 OS=Cucumis melo OX=3656 GN=LOC103498704 PE=4 SV=1)

HSP 1 Score: 790.4 bits (2040), Expect = 4.7e-225
Identity = 401/537 (74.67%), Postives = 456/537 (84.92%), Query Frame = 0

Query: 1   MASGFSILNGTLDISQWVHQINDLIDQNALPAASQTPISVFRLPESVHAGDPTAYLPHHV 60
           MASGFSIL GT D SQWV QIN+L+DQNAL  A+QTPISVFRLPESVH GDPTAYLP HV
Sbjct: 1   MASGFSILTGTFDKSQWVLQINNLVDQNALLDATQTPISVFRLPESVHDGDPTAYLPRHV 60

Query: 61  ALGPYHHFRPELYKMDLFKLGKAKNLKWGPQIHKLADQLKPLELKIRACFDQGHEMNGET 120
           A GP+HHF+PEL+KM+LFKLGKAKNLKWGPQIHKLAD+L PLELK+RACFDQ  E+NGET
Sbjct: 61  AFGPFHHFQPELHKMELFKLGKAKNLKWGPQIHKLADRLTPLELKMRACFDQALEINGET 120

Query: 121 LSWVVLIDGLFLLDLLQKKFLNRPLKFPLDIFYGKLWSEFEIVSDIVKLENQIPLFVLNE 180
           LSWV+LIDGLFL+ LLQ K++N PL+FP +IFYGKLWSEFEIV D+VKLENQIPLFVLNE
Sbjct: 121 LSWVLLIDGLFLIYLLQNKYMNCPLRFPSEIFYGKLWSEFEIVCDMVKLENQIPLFVLNE 180

Query: 181 ICPKEPINYVAPFLYQFSASVSPFQLPCFNPELECFGFSSYLPEIFNLSHHLLHFLYSLV 240
           ICPKEPIN++APFLYQF  SVSPF LP FNP+LECFGFSSYLPEIF+LSHHLLHFLYSL+
Sbjct: 181 ICPKEPINFLAPFLYQFCVSVSPFPLPRFNPQLECFGFSSYLPEIFDLSHHLLHFLYSLI 240

Query: 241 LVIPVEVPISNRNRTRMNWENSSSSSSSVEFLIECFNILGSVINIAFFQQLKDTFGLIQR 300
           L+IP E  +      R++    +SSSSSVEFL E  +ILGSVINIAF QQ+K+T GLIQR
Sbjct: 241 LLIPDERVVIMLFGLRLD----NSSSSSVEFLSESLDILGSVINIAFIQQIKETIGLIQR 300

Query: 301 LFRLLGLITKTNSAERKTPLIIPSASDLKTAGVTFKSTKTGILKCHFDKTTLTLTLPSIH 360
           L RLL  ITK N AE+  PLIIPSAS LK+AG TFKSTK GILK +FD+TTLTLTLP IH
Sbjct: 301 LLRLLSSITKPNLAEKTPPLIIPSASHLKSAGFTFKSTKNGILKSYFDETTLTLTLPCIH 360

Query: 361 LDGFSQVLLKNLVAFEAMAELNPPCFVNYTALMNGLLRNSKDLKILEKAQIVHNHLDSEE 420
           L GF+ VLLKNLVAFE+MAELNPPC  NYTALMNGLLRNSKDLK+LEKA+IVHNHL+SEE
Sbjct: 361 LHGFTHVLLKNLVAFESMAELNPPCLANYTALMNGLLRNSKDLKVLEKAEIVHNHLNSEE 420

Query: 421 EAVELFNGVENSTSSLKKERVLKIHLNSGLNSGNRSMFIRDVKISYEMGLGAMADEINKF 480
           EA ELF GVENST  LKKE +L+IHL  G N  N S FI++V+ SYEMGLG M + IN  
Sbjct: 421 EAAELFYGVENSTRRLKKESLLEIHLKGGFNFENGSNFIQEVESSYEMGLGNMVERINNC 480

Query: 481 YANCWRMKMKKFMSVVFKCFTILVIVFVIVLVIVRFVCNFLTCPVGIFKTSISLHQM 538
           Y NCWRMKMKK +SVVFKCF +L++VF+IVLV  RFVCNF++CP+G F T+ +LHQM
Sbjct: 481 YGNCWRMKMKKVVSVVFKCFAVLIVVFLIVLVTTRFVCNFMSCPLGTFMTTTALHQM 533

BLAST of Lsi08G000460 vs. ExPASy TrEMBL
Match: A0A6J1GBT5 (putative UPF0481 protein At3g02645 OS=Cucurbita moschata OX=3662 GN=LOC111452550 PE=4 SV=1)

HSP 1 Score: 773.9 bits (1997), Expect = 4.5e-220
Identity = 401/533 (75.23%), Postives = 451/533 (84.62%), Query Frame = 0

Query: 6   SILNGTLDISQWVHQINDLIDQNALPAASQTPISVFRLPESVHAGDPTAYLPHHVALGPY 65
           SILNGTL  SQWV QINDLID+NALPAAS+TPISVF+LPESV  GDP AYLP  +ALGPY
Sbjct: 2   SILNGTLQKSQWVLQINDLIDRNALPAASRTPISVFQLPESVRDGDPAAYLPQFIALGPY 61

Query: 66  HHFRPELYKMDLFKLGKAKNLKWGPQIHKLADQLKPLELKIRACFDQGHEMNGETLSWVV 125
           HHFRPELYKMDLFKLGKAKNLKW PQ+H+LADQLKPLEL+IR CF    E++ ETLSWVV
Sbjct: 62  HHFRPELYKMDLFKLGKAKNLKWRPQLHELADQLKPLELQIRGCFCGSLEIDDETLSWVV 121

Query: 126 LIDGLFLLDLLQKKFLNRPLKFPLDIFYGKLWSEFEIVSDIVKLENQIPLFVLNEICPKE 185
           LIDGLFL+ LLQ +F NRPLK PL+IFYGKLWSEFEIVSD++KLENQIPL VL EICP E
Sbjct: 122 LIDGLFLIHLLQNEFSNRPLKLPLEIFYGKLWSEFEIVSDMMKLENQIPLLVLKEICPDE 181

Query: 186 PINYVAPFLYQFSASVSPFQLPCFNPELECFGFSSYLPEIFNLSHHLLHFLYSLVLVIPV 245
           P NY+AP  YQFSASVSPF       EL CFGF+S LPEIF+ S+HLLHF+Y L+L+IP 
Sbjct: 182 PFNYLAPLFYQFSASVSPF-------ELVCFGFASNLPEIFDSSYHLLHFVYFLILLIPD 241

Query: 246 EVPISNRNRTRMNWENSSSSSSSVEFLIECFNILGSVINIAFFQQLKDTFGLIQRLFRLL 305
           E+ ++N     M      SSSSSVEFL E  NILGSVINIAF QQ+K+TFGLIQRLFRLL
Sbjct: 242 EMAVANICAYSMP-IGMDSSSSSVEFLGESLNILGSVINIAFIQQVKETFGLIQRLFRLL 301

Query: 306 GLITKTNSAERKTPLIIPSASDLKTAGVTFKSTKTGILKCHFDKTTLTLTLPSIHLDGFS 365
           G ITK N  E+K P+IIPSASDLKTAG+TFKSTK G+LKC FD+ TLTLTLPSIHLDGFS
Sbjct: 302 GSITKPNLCEKKLPMIIPSASDLKTAGITFKSTKNGVLKCRFDEKTLTLTLPSIHLDGFS 361

Query: 366 QVLLKNLVAFEAMAELNPPCFVNYTALMNGLLRNSKDLKILEKAQIVHNHLDSEEEAVEL 425
           +VLLKNLVAFEAMAELNP C VNY ALM GLLRNSKDLKIL KA+IV NHL SEE+AVE+
Sbjct: 362 EVLLKNLVAFEAMAELNPQCLVNYIALMKGLLRNSKDLKILVKAEIVDNHLSSEEQAVEV 421

Query: 426 FNGVENSTSSLKKERVLKIHLNSGLNSGNRSMFIRDVKISYEMGLGAMADEINKFYANCW 485
           FNGVENSTSSLKKE VL IHL SGL+    S FI+D+K++YEMGLG+M +EINK+Y +CW
Sbjct: 422 FNGVENSTSSLKKEEVLNIHLKSGLDCEIGSGFIQDIKMNYEMGLGSMVEEINKWYGSCW 481

Query: 486 RMKMKKFMSVVFKCFTILVIVFVIVLVIVRFVCNFLTCPVGIFKTSISLHQMY 539
           RMKMKKFMSVVFKCF +LV+VFVIVLVI RFVCNF++C VG+FKTS +LHQMY
Sbjct: 482 RMKMKKFMSVVFKCFAVLVVVFVIVLVIARFVCNFVSCSVGMFKTSDALHQMY 526

BLAST of Lsi08G000460 vs. ExPASy TrEMBL
Match: A0A6J1IBB2 (putative UPF0481 protein At3g02645 OS=Cucurbita maxima OX=3661 GN=LOC111471968 PE=4 SV=1)

HSP 1 Score: 773.5 bits (1996), Expect = 5.9e-220
Identity = 398/533 (74.67%), Postives = 450/533 (84.43%), Query Frame = 0

Query: 6   SILNGTLDISQWVHQINDLIDQNALPAASQTPISVFRLPESVHAGDPTAYLPHHVALGPY 65
           SILNGTL+ SQWV QI D+I QNALPAAS TPISVF+LPESV  GDP AYLP  +ALGPY
Sbjct: 2   SILNGTLEKSQWVLQIFDIIHQNALPAASHTPISVFQLPESVRDGDPAAYLPQFIALGPY 61

Query: 66  HHFRPELYKMDLFKLGKAKNLKWGPQIHKLADQLKPLELKIRACFDQGHEMNGETLSWVV 125
           HHFR ELYKMDLFKLGKAKNLKW PQ+H+LADQLKPLEL+IR CF    E++GETLSWVV
Sbjct: 62  HHFRSELYKMDLFKLGKAKNLKWRPQLHELADQLKPLELQIRGCFCGSLEIDGETLSWVV 121

Query: 126 LIDGLFLLDLLQKKFLNRPLKFPLDIFYGKLWSEFEIVSDIVKLENQIPLFVLNEICPKE 185
           LIDGLFL+DLLQ +F NRPLK PL+IFYGKLWSEFEIVSD+VKLENQIPL VL EICP E
Sbjct: 122 LIDGLFLIDLLQNEFSNRPLKLPLEIFYGKLWSEFEIVSDMVKLENQIPLLVLQEICPDE 181

Query: 186 PINYVAPFLYQFSASVSPFQLPCFNPELECFGFSSYLPEIFNLSHHLLHFLYSLVLVIPV 245
           P NY+AP  YQFSASVSPF       EL CFGF+S LPEIF+ SHHLLHF+Y L+L+IP 
Sbjct: 182 PFNYLAPLFYQFSASVSPF-------ELVCFGFASNLPEIFDSSHHLLHFVYFLILLIPD 241

Query: 246 EVPISNRNRTRMNWENSSSSSSSVEFLIECFNILGSVINIAFFQQLKDTFGLIQRLFRLL 305
           E+ ++N     M     SSSSSSVE L E  NILGSVI+IAF QQ+K+TFGLI+RLFRLL
Sbjct: 242 EMAVANICAYSMPIGMDSSSSSSVELLGESLNILGSVISIAFIQQVKETFGLIRRLFRLL 301

Query: 306 GLITKTNSAERKTPLIIPSASDLKTAGVTFKSTKTGILKCHFDKTTLTLTLPSIHLDGFS 365
           G ITK N  E+K P+IIPSASDLKTAG+TFKST  G+LKC FD+ TLTLTLPSIHLD FS
Sbjct: 302 GSITKPNLCEKKLPMIIPSASDLKTAGITFKSTNNGVLKCRFDEKTLTLTLPSIHLDWFS 361

Query: 366 QVLLKNLVAFEAMAELNPPCFVNYTALMNGLLRNSKDLKILEKAQIVHNHLDSEEEAVEL 425
           +VLLKNLVAFEAMAELNP C VNY ALM GLLRNSKDLKILEKA+IVHNHL +EE+AVE+
Sbjct: 362 EVLLKNLVAFEAMAELNPQCLVNYIALMKGLLRNSKDLKILEKAEIVHNHLSNEEQAVEV 421

Query: 426 FNGVENSTSSLKKERVLKIHLNSGLNSGNRSMFIRDVKISYEMGLGAMADEINKFYANCW 485
           FNGVENSTSSLKKE VLKIHL SGL+    S FI+D+K++YEMGLG+M +EINK+Y +CW
Sbjct: 422 FNGVENSTSSLKKEEVLKIHLKSGLDCEIGSGFIQDIKMNYEMGLGSMVEEINKWYGSCW 481

Query: 486 RMKMKKFMSVVFKCFTILVIVFVIVLVIVRFVCNFLTCPVGIFKTSISLHQMY 539
           RMKMK+FMSVVFKCF +LV+VFVIVLVI RF CNF++C VG+FKTS +LHQMY
Sbjct: 482 RMKMKRFMSVVFKCFAVLVVVFVIVLVIARFACNFVSCSVGMFKTSDALHQMY 527

BLAST of Lsi08G000460 vs. NCBI nr
Match: XP_038890580.1 (putative UPF0481 protein At3g02645 [Benincasa hispida])

HSP 1 Score: 818.9 bits (2114), Expect = 2.5e-233
Identity = 417/530 (78.68%), Postives = 464/530 (87.55%), Query Frame = 0

Query: 11  TLDISQWVHQINDLIDQNALPAASQTPISVFRLPESVHAGDPTAYLPHHVALGPYHHFRP 70
           ++D SQWVHQINDLIDQNAL  ASQTPISV RLPESVH G+P A+LP HVA GPYHHFRP
Sbjct: 3   SVDKSQWVHQINDLIDQNALLPASQTPISVVRLPESVHDGNPAAFLPRHVAFGPYHHFRP 62

Query: 71  ELYKMDLFKLGKAKNLKWGPQIHKLADQLKPLELKIRACFDQGHEMNGETLSWVVLIDGL 130
           ELY M+LFKLGKAKNL W PQ+HKLADQL PLELKIRACFDQ  E+NGETLSWV+LIDGL
Sbjct: 63  ELYNMELFKLGKAKNLNWSPQLHKLADQLMPLELKIRACFDQTLEINGETLSWVLLIDGL 122

Query: 131 FLLDLLQKKFLNRPLKFPLDIFYGKLWSEFEIVSDIVKLENQIPLFVLNEICPKEPINYV 190
           FL+ LLQ K+ N PL+FPL I+YGKLWSEFEIVSD+VKLENQIPLFVLNEICPKEPIN++
Sbjct: 123 FLIHLLQNKYNNHPLEFPLGIYYGKLWSEFEIVSDMVKLENQIPLFVLNEICPKEPINFL 182

Query: 191 APFLYQFSASVSPFQLPCFNPELECFGFSSYLPEIFNLSHHLLHFLYSLVLVIPVEVPIS 250
           APF+YQFSASVSPFQLP FNPELECFGFSS LPEIFNLSHHLLHFLYSL+L+IP EV ++
Sbjct: 183 APFMYQFSASVSPFQLPIFNPELECFGFSSNLPEIFNLSHHLLHFLYSLILLIPDEVVVA 242

Query: 251 NRNRTRMNWENSSSSSSSVEFLIECFNILGSVINIAFFQQLKDTFGLIQRLFRLLGLITK 310
              R        +SS+++ E L E  NILGSVINIAF QQ+K+TFGLIQRLFRLL  ITK
Sbjct: 243 YCMRL------DTSSAAAAELLSESLNILGSVINIAFIQQVKETFGLIQRLFRLLSSITK 302

Query: 311 TNSAERKTP--LIIPSASDLKTAGVTFKSTKTGILKCHFDKTTLTLTLPSIHLDGFSQVL 370
            N AE+  P  LIIPSASDLK+AGVTFKSTK GILKC F+++TLTLTLP IHLDGFSQVL
Sbjct: 303 PNLAEKHHPPLLIIPSASDLKSAGVTFKSTKNGILKCFFNESTLTLTLPCIHLDGFSQVL 362

Query: 371 LKNLVAFEAMAELNPPCFVNYTALMNGLLRNSKDLKILEKAQIVHNHLDSEEEAVELFNG 430
           LKNLVAFEAM ELNPPC VNY +LMNGLLRNSKDLK+LEKA IVHNHL+SEEEAVELFNG
Sbjct: 363 LKNLVAFEAMTELNPPCLVNYVSLMNGLLRNSKDLKVLEKAGIVHNHLNSEEEAVELFNG 422

Query: 431 VENSTSSLKKERVLKIHLNSGLNSGNRSMFIRDVKISYEMGLGAMADEINKFYANCWRMK 490
           VENST SLKKE VLKIHL SGLN    S FI+DVK+SYEMGLGA+A+EINKFY NCWRMK
Sbjct: 423 VENSTRSLKKESVLKIHLKSGLNFETGSKFIQDVKMSYEMGLGALAEEINKFYGNCWRMK 482

Query: 491 MKKFMSVVFKCFTILVIVFVIVLVIVRFVCNFLTCPVGIFKTSISLHQMY 539
           MKKFMS+VFKCF IL++VF+IVLVI RFVCNFL+CP+GIFKTSI+LH+M+
Sbjct: 483 MKKFMSIVFKCFAILLVVFLIVLVIARFVCNFLSCPLGIFKTSIALHKMF 526

BLAST of Lsi08G000460 vs. NCBI nr
Match: XP_038890866.1 (putative UPF0481 protein At3g02645 [Benincasa hispida])

HSP 1 Score: 805.4 bits (2079), Expect = 2.9e-229
Identity = 414/538 (76.95%), Postives = 462/538 (85.87%), Query Frame = 0

Query: 2   ASGFSILNGTLDISQWVHQINDLIDQNALPAASQTPISVFRLPESVHAGDPTAYLPHHVA 61
           +SGFSILNGTLD S WV QINDLI QNALPAAS TPIS+FRLPESVH GD  +YLP HVA
Sbjct: 3   SSGFSILNGTLDKSHWVLQINDLIHQNALPAASHTPISIFRLPESVHDGDQASYLPCHVA 62

Query: 62  LGPYHHFRPELYKMDLFKLGKAKNLKWGPQIHKLADQLKPLELKIRACFDQGHEMNGETL 121
           LGPYHHFRPEL KM+LFKLGKAKNL W PQ+H+LADQLKPLELKIRACFDQ  E++GETL
Sbjct: 63  LGPYHHFRPELSKMELFKLGKAKNLNWDPQLHELADQLKPLELKIRACFDQALEISGETL 122

Query: 122 SWVVLIDGLFLLD-LLQKKFLNRPLKFPLDIFYGKLWSEFEIVSDIVKLENQIPLFVLNE 181
           SWV+LIDGLFL+  LLQ K+  RP +FPL IFYGKL SEFEIVSD+VKLENQIPLFVLNE
Sbjct: 123 SWVLLIDGLFLIHLLLQNKYRCRPFQFPLQIFYGKLSSEFEIVSDMVKLENQIPLFVLNE 182

Query: 182 ICPKEPINYVAPFLYQFSASVSPFQLPCFNPELECFGFSSYLPEIFNLSHHLLHFLYSLV 241
           ICPKE  N++AP LYQFSASVSP QLP FNP LECFGFSSYLPEIF+LSHHLLHFLY L+
Sbjct: 183 ICPKESNNFLAPLLYQFSASVSPIQLPYFNPRLECFGFSSYLPEIFDLSHHLLHFLYYLI 242

Query: 242 LVIPVEVPISNRNRTRMNWENSSSSSSSVEFLIECFNILGSVINIAFFQQLKDTFGLIQR 301
           L+IP E+ +SN    R + ENSS SSSSVEF+ +CF ILGSVIN+AF QQ+K+TFGLIQR
Sbjct: 243 LLIPEEIDVSNHR--RRDRENSSPSSSSVEFVSQCFEILGSVINLAFIQQVKETFGLIQR 302

Query: 302 LFRLLGLITKTNSAERKTPLIIPSASDLKTAGVTFKSTKTGILKCHFDKTTLTLTLPSIH 361
           +++LLG ITK+N  E+K  LIIPSASDLKTAG TFKST  GILK  FDK T TLTLP IH
Sbjct: 303 VYKLLGFITKSNLVEKKPLLIIPSASDLKTAGFTFKSTGAGILKSRFDKETRTLTLPCIH 362

Query: 362 LDGFSQVLLKNLVAFEAMAELNPPCFVNYTALMNGLLRNSKDLKILEKAQIVHNHLDSEE 421
           LDGFSQV LKNLVAFEAMAELNPPC   YTA++NGLLRNSKDLKILEKA+IV NHL+SEE
Sbjct: 363 LDGFSQVFLKNLVAFEAMAELNPPCLTKYTAVINGLLRNSKDLKILEKAKIVRNHLNSEE 422

Query: 422 EAVELFNGVENSTSSLKKERVLKIHLNSGLNSGNRSMFIRDVKISYEMGLGAMADEINKF 481
           EAVELFNGVENSTSSLKK+R+LKIHL  G  S     F++DVK +YE+GLGAMA+ INK 
Sbjct: 423 EAVELFNGVENSTSSLKKDRLLKIHLKGGFVSEYGGKFVQDVKRNYELGLGAMAEGINKI 482

Query: 482 YANCWRMKMKKFMSVVFKCFTILVIVFVIVLVIVRFVCNFLTCPVGIFKTSISLHQMY 539
           Y NCWRMKMKKFMS+VFKCF ILV+VF+IVLVI RF+C FLTCPVGIF+T I+LHQMY
Sbjct: 483 YDNCWRMKMKKFMSLVFKCFAILVLVFLIVLVIARFLCKFLTCPVGIFQTVIALHQMY 538

BLAST of Lsi08G000460 vs. NCBI nr
Match: XP_004141645.1 (putative UPF0481 protein At3g02645 [Cucumis sativus] >KGN52693.1 hypothetical protein Csa_008317 [Cucumis sativus])

HSP 1 Score: 802.7 bits (2072), Expect = 1.9e-228
Identity = 409/538 (76.02%), Postives = 461/538 (85.69%), Query Frame = 0

Query: 1   MASGFSILNGTLDISQWVHQINDLIDQNALPAASQTPISVFRLPESVHAGDPTAYLPHHV 60
           MASGFSIL GT D SQW+HQIN+L++Q+AL AASQ PISVFRLPESVH  DPTAYLP HV
Sbjct: 1   MASGFSILTGTFDKSQWLHQINNLVNQDALLAASQIPISVFRLPESVHDVDPTAYLPRHV 60

Query: 61  ALGPYHHFRPELYKMDLFKLGKAKNLKWGPQIHKLADQLKPLELKIRACFDQGHEMNGET 120
           A GP+HHF+PELYKM+LFKLGKAKNLKWGPQIHKL+D+L PLELKIRACFDQ  E+NGET
Sbjct: 61  AFGPFHHFQPELYKMELFKLGKAKNLKWGPQIHKLSDRLTPLELKIRACFDQALEINGET 120

Query: 121 LSWVVLIDGLFLLDLLQKKFLNRPLKFPLDIFYGKLWSEFEIVSDIVKLENQIPLFVLNE 180
           LSW++LIDGLFL+ LLQ +++N PL FP +IFYGKLWSEFEIV D+VKLENQIPLFVLNE
Sbjct: 121 LSWLLLIDGLFLIYLLQNEYMNCPLTFPSEIFYGKLWSEFEIVCDMVKLENQIPLFVLNE 180

Query: 181 ICPKEPINYVAPFLYQFSASVSPFQLPCFNPELECFGFSSYLPEIFNLSHHLLHFLYSLV 240
           ICP+EPIN++AP LYQF  SVSPFQLPCFNP LECFGFSSYLPEIF+LSHHLLHFLYSL+
Sbjct: 181 ICPEEPINFLAPSLYQFCVSVSPFQLPCFNPRLECFGFSSYLPEIFDLSHHLLHFLYSLI 240

Query: 241 LVIPVEVPISNRNRTRMNWENSSSSSSSVEFLIECFNILGSVINIAFFQQLKDTFGLIQR 300
           L+IP E  +   +  RM  +NSSSSSSSVEFL EC +ILGSVINIAF QQ+K+T GLIQR
Sbjct: 241 LLIPNERVVFVAHCFRM--DNSSSSSSSVEFLSECLDILGSVINIAFIQQIKETIGLIQR 300

Query: 301 LFRLLGLITKTNSAERKTPLIIPSASDLKTAGVTFKSTKTGILKCHFDKTTLTLTLPSIH 360
           L RLL  ITK N AE+   LIIPSASDLK+AG TFKSTK GILK +FD+TTLTLTLP IH
Sbjct: 301 LLRLLSFITKPNLAEKTPLLIIPSASDLKSAGFTFKSTKNGILKSNFDETTLTLTLPCIH 360

Query: 361 LDGFSQVLLKNLVAFEAMAELNPPCFVNYTALMNGLLRNSKDLKILEKAQIVHNHLDSEE 420
           LDGF+ VLLKNLVAFEAMAELNPPC  NYTALMNGLLRNSKDLK+LEKA+IVHNHL+SEE
Sbjct: 361 LDGFTHVLLKNLVAFEAMAELNPPCLANYTALMNGLLRNSKDLKVLEKAEIVHNHLNSEE 420

Query: 421 EAVELFNGVENSTSSLKKERVLKIHLNSGLNSGNRSMFIRDVKISY-EMGLGAMADEINK 480
           EA ELF GVENSTS LKK  +LKIHLNSG N  N S FI++V+ SY EMGLG M + IN 
Sbjct: 421 EAAELFYGVENSTSKLKKGSLLKIHLNSGFNFENGSKFIQEVESSYIEMGLGDMVERINN 480

Query: 481 FYANCWRMKMKKFMSVVFKCFTILVIVFVIVLVIVRFVCNFLTCPVGIFKTSISLHQM 538
            Y NCWRMKMKKF+SVV+KCF +LV+VF+IVLV  RFVCNFL+CP G F  + +LHQM
Sbjct: 481 CYGNCWRMKMKKFVSVVYKCFAVLVVVFLIVLVTTRFVCNFLSCPFGTFMNTTALHQM 536

BLAST of Lsi08G000460 vs. NCBI nr
Match: KAA0039249.1 (putative UPF0481 protein [Cucumis melo var. makuwa] >TYK00435.1 putative UPF0481 protein [Cucumis melo var. makuwa])

HSP 1 Score: 792.7 bits (2046), Expect = 1.9e-225
Identity = 403/537 (75.05%), Postives = 455/537 (84.73%), Query Frame = 0

Query: 1   MASGFSILNGTLDISQWVHQINDLIDQNALPAASQTPISVFRLPESVHAGDPTAYLPHHV 60
           MASGFSIL GT D SQWV QINDL+DQNAL  A+QTPIS+FRLPESVH GDPTAYLP HV
Sbjct: 1   MASGFSILTGTFDKSQWVLQINDLVDQNALLDATQTPISIFRLPESVHDGDPTAYLPRHV 60

Query: 61  ALGPYHHFRPELYKMDLFKLGKAKNLKWGPQIHKLADQLKPLELKIRACFDQGHEMNGET 120
           A GP+HHF+PELYKM+LFKLGKAKNLKWGPQIHKLAD+L PLELK+RACFDQ  E+NGET
Sbjct: 61  AFGPFHHFQPELYKMELFKLGKAKNLKWGPQIHKLADRLTPLELKMRACFDQALEINGET 120

Query: 121 LSWVVLIDGLFLLDLLQKKFLNRPLKFPLDIFYGKLWSEFEIVSDIVKLENQIPLFVLNE 180
           LSWV+LIDGLFL+ LLQ K++NRPL+FP +IFYGKLWSEFEIV D+VKLENQIPLFVLNE
Sbjct: 121 LSWVLLIDGLFLIYLLQNKYMNRPLRFPSEIFYGKLWSEFEIVGDMVKLENQIPLFVLNE 180

Query: 181 ICPKEPINYVAPFLYQFSASVSPFQLPCFNPELECFGFSSYLPEIFNLSHHLLHFLYSLV 240
           ICPKEPIN++APFLYQF  SVS F LP FNP+LECFGFSSYLPEIF+LSHHLLHFLYSL+
Sbjct: 181 ICPKEPINFLAPFLYQFCVSVSSFPLPRFNPQLECFGFSSYLPEIFDLSHHLLHFLYSLI 240

Query: 241 LVIPVEVPISNRNRTRMNWENSSSSSSSVEFLIECFNILGSVINIAFFQQLKDTFGLIQR 300
           L+IP E         R++    +SSSSSVEFL E  +ILGSVINIAF QQ+K+T GLIQR
Sbjct: 241 LLIPDERVAFMPYCFRLD----NSSSSSVEFLSESLDILGSVINIAFIQQIKETIGLIQR 300

Query: 301 LFRLLGLITKTNSAERKTPLIIPSASDLKTAGVTFKSTKTGILKCHFDKTTLTLTLPSIH 360
           L RLL  ITK N AE+  PLIIPSASDLK+AG TFKSTK GILK +FD+TTLTLTLP IH
Sbjct: 301 LLRLLSSITKPNLAEKTPPLIIPSASDLKSAGFTFKSTKNGILKSYFDETTLTLTLPCIH 360

Query: 361 LDGFSQVLLKNLVAFEAMAELNPPCFVNYTALMNGLLRNSKDLKILEKAQIVHNHLDSEE 420
           L GF+ VLLKNLVAFE+MAELNPPC  NYTALMNGLLR SKDLK+LEKA+IVHNHL+SEE
Sbjct: 361 LHGFTHVLLKNLVAFESMAELNPPCLANYTALMNGLLRKSKDLKVLEKAEIVHNHLNSEE 420

Query: 421 EAVELFNGVENSTSSLKKERVLKIHLNSGLNSGNRSMFIRDVKISYEMGLGAMADEINKF 480
           EA ELF GVENSTS LKKE +L+IHL  G N  N S FI +V+ SYEMGLG M + IN  
Sbjct: 421 EAAELFYGVENSTSRLKKESLLEIHLKGGFNFENGSNFIPEVESSYEMGLGNMVERINNC 480

Query: 481 YANCWRMKMKKFMSVVFKCFTILVIVFVIVLVIVRFVCNFLTCPVGIFKTSISLHQM 538
           Y NCWRMKMKK +SVV+KCF +LV+VF+IVLV  RFVCNF++CP+G F T+ +LHQM
Sbjct: 481 YGNCWRMKMKKVVSVVYKCFAVLVVVFLIVLVTTRFVCNFMSCPLGTFMTTTALHQM 533

BLAST of Lsi08G000460 vs. NCBI nr
Match: XP_008459642.1 (PREDICTED: putative UPF0481 protein At3g02645 [Cucumis melo])

HSP 1 Score: 790.4 bits (2040), Expect = 9.6e-225
Identity = 401/537 (74.67%), Postives = 456/537 (84.92%), Query Frame = 0

Query: 1   MASGFSILNGTLDISQWVHQINDLIDQNALPAASQTPISVFRLPESVHAGDPTAYLPHHV 60
           MASGFSIL GT D SQWV QIN+L+DQNAL  A+QTPISVFRLPESVH GDPTAYLP HV
Sbjct: 1   MASGFSILTGTFDKSQWVLQINNLVDQNALLDATQTPISVFRLPESVHDGDPTAYLPRHV 60

Query: 61  ALGPYHHFRPELYKMDLFKLGKAKNLKWGPQIHKLADQLKPLELKIRACFDQGHEMNGET 120
           A GP+HHF+PEL+KM+LFKLGKAKNLKWGPQIHKLAD+L PLELK+RACFDQ  E+NGET
Sbjct: 61  AFGPFHHFQPELHKMELFKLGKAKNLKWGPQIHKLADRLTPLELKMRACFDQALEINGET 120

Query: 121 LSWVVLIDGLFLLDLLQKKFLNRPLKFPLDIFYGKLWSEFEIVSDIVKLENQIPLFVLNE 180
           LSWV+LIDGLFL+ LLQ K++N PL+FP +IFYGKLWSEFEIV D+VKLENQIPLFVLNE
Sbjct: 121 LSWVLLIDGLFLIYLLQNKYMNCPLRFPSEIFYGKLWSEFEIVCDMVKLENQIPLFVLNE 180

Query: 181 ICPKEPINYVAPFLYQFSASVSPFQLPCFNPELECFGFSSYLPEIFNLSHHLLHFLYSLV 240
           ICPKEPIN++APFLYQF  SVSPF LP FNP+LECFGFSSYLPEIF+LSHHLLHFLYSL+
Sbjct: 181 ICPKEPINFLAPFLYQFCVSVSPFPLPRFNPQLECFGFSSYLPEIFDLSHHLLHFLYSLI 240

Query: 241 LVIPVEVPISNRNRTRMNWENSSSSSSSVEFLIECFNILGSVINIAFFQQLKDTFGLIQR 300
           L+IP E  +      R++    +SSSSSVEFL E  +ILGSVINIAF QQ+K+T GLIQR
Sbjct: 241 LLIPDERVVIMLFGLRLD----NSSSSSVEFLSESLDILGSVINIAFIQQIKETIGLIQR 300

Query: 301 LFRLLGLITKTNSAERKTPLIIPSASDLKTAGVTFKSTKTGILKCHFDKTTLTLTLPSIH 360
           L RLL  ITK N AE+  PLIIPSAS LK+AG TFKSTK GILK +FD+TTLTLTLP IH
Sbjct: 301 LLRLLSSITKPNLAEKTPPLIIPSASHLKSAGFTFKSTKNGILKSYFDETTLTLTLPCIH 360

Query: 361 LDGFSQVLLKNLVAFEAMAELNPPCFVNYTALMNGLLRNSKDLKILEKAQIVHNHLDSEE 420
           L GF+ VLLKNLVAFE+MAELNPPC  NYTALMNGLLRNSKDLK+LEKA+IVHNHL+SEE
Sbjct: 361 LHGFTHVLLKNLVAFESMAELNPPCLANYTALMNGLLRNSKDLKVLEKAEIVHNHLNSEE 420

Query: 421 EAVELFNGVENSTSSLKKERVLKIHLNSGLNSGNRSMFIRDVKISYEMGLGAMADEINKF 480
           EA ELF GVENST  LKKE +L+IHL  G N  N S FI++V+ SYEMGLG M + IN  
Sbjct: 421 EAAELFYGVENSTRRLKKESLLEIHLKGGFNFENGSNFIQEVESSYEMGLGNMVERINNC 480

Query: 481 YANCWRMKMKKFMSVVFKCFTILVIVFVIVLVIVRFVCNFLTCPVGIFKTSISLHQM 538
           Y NCWRMKMKK +SVVFKCF +L++VF+IVLV  RFVCNF++CP+G F T+ +LHQM
Sbjct: 481 YGNCWRMKMKKVVSVVFKCFAVLIVVFLIVLVTTRFVCNFMSCPLGTFMTTTALHQM 533

BLAST of Lsi08G000460 vs. TAIR 10
Match: AT3G02645.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 161.4 bits (407), Expect = 2.0e-39
Identity = 139/540 (25.74%), Postives = 250/540 (46.30%), Query Frame = 0

Query: 13  DISQWVHQINDLIDQNALP-AASQTPISVFRLPESVHAGDPTAYLPHHVALGPYHHFRPE 72
           D ++WV  +   +D         +  +S+F +P+++    P +Y PH V++GPYH  +PE
Sbjct: 17  DETRWVINVQKSLDAELEEHDLEEVTVSIFNVPKALMCSHPDSYTPHRVSIGPYHCLKPE 76

Query: 73  LYKMDLFKL---GKAKNLKWGPQIHKLADQLKPLELKIRACFDQGHEMNGETLSWVVLID 132
           L++M+ +KL    K +N     + H L ++L+ +E+KIRAC+ +    NGETL W++ +D
Sbjct: 77  LHEMERYKLMIARKIRNQYNSFRFHDLVEKLQSMEIKIRACYHKYIGFNGETLLWIMAVD 136

Query: 133 GLFLLDLLQKKFLNRPLKFPLDIFYGKLWSEFEIVSDIVKLENQIPLFVLNEICPKEPIN 192
             FL++ L+     +     ++    ++    EI+ DI+ +ENQIPLFVL +        
Sbjct: 137 SSFLIEFLKIYSFRK-----VETLINRV-GHNEILRDIMMIENQIPLFVLRK-------- 196

Query: 193 YVAPFLYQFSASVSPFQLPC-------FNPELECFGFSSYLPEIFNLSHHLLHFLYSLVL 252
               F  + + S     L          +P +  F     L   F   +H+L FLY +++
Sbjct: 197 -TLEFQLESTESADDLLLSVLTGLCKDLSPLVIKFDDDQILKAQFQECNHILDFLYQMIV 256

Query: 253 VIPVEVPISNRNRTRMNWENSSSSSSSVEFLIECFNILGSVI-----------------N 312
               E  +   +    N  + +  + ++ F+ E  +    V                  N
Sbjct: 257 PRIEEEELEEDDEE--NRADENGGNRAIRFMDEIKHQFKRVFASRPADLILRFPWRIISN 316

Query: 313 IAFFQQLKDTFGLIQRLFRLLGLITKTNSAE----RKTPLI----IPSASDLKTAGVTFK 372
           +  F  LK +   +          T+  S       K PL+    IPS SDL  AGV FK
Sbjct: 317 LPGFMALKLSADYLFTRQENEATTTRQESVSILDIEKPPLVEELTIPSVSDLHKAGVRFK 376

Query: 373 STKTG-ILKCHFDKTTLTLTLPSIHLDGFSQVLLKNLVAFEAMAELNPPCFVNYTALMNG 432
            T  G I    FD  +    LP I+LD  ++ +L+NLVA+EA     P  F  YT L+NG
Sbjct: 377 PTAHGNISTVTFDSNSGQFYLPVINLDINTETVLRNLVAYEATNTSGPLVFTRYTELING 436

Query: 433 LLRNSKDLKILEKAQIVHNHLDSEEEAVELFNGVENSTSSLKKERVLKIHLNSGLNSGNR 492
           ++ + +D+++L +  ++ + L S++EA E++NG+  S                       
Sbjct: 437 IIDSEEDVRLLREQGVLVSRLKSDQEAAEMWNGMSKS----------------------- 496

Query: 493 SMFIRDVKISYEMGLGAMADEINKFYANCWRMKMKKFMSV-VFKCFTILVIVFVIVLVIV 515
              +R  K+ +   L    +++N++Y   W++K+ + + V V+  + IL  +  ++L+++
Sbjct: 497 ---VRLTKVGF---LDKTIEDVNRYYTGRWKVKIGRLVEVYVYGSWQILAFLAAVLLLML 510

BLAST of Lsi08G000460 vs. TAIR 10
Match: AT3G50130.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 93.6 bits (231), Expect = 5.2e-19
Identity = 101/430 (23.49%), Postives = 177/430 (41.16%), Query Frame = 0

Query: 16  QWVHQINDLIDQNALPAA--SQTPISVFRLPESVHAGDPTAYLPHHVALGPYHHFRPELY 75
           +WV  I D ++Q     A  S   + ++R+P+ +   +  +Y P  V+LGP+HH    L 
Sbjct: 115 EWVISIRDKMEQALREDATTSWDKLCIYRVPQYLQENNKKSYFPQTVSLGPFHHGNKHLL 174

Query: 76  KMDLFKLGKAKNLKWGPQIHKL---ADQLKPLELKIRACFDQGHEMNGETLSWVVLIDGL 135
            MD  K  +A N+      H +    D +K LE + RAC++   +++    S ++++DG 
Sbjct: 175 PMDRHK-WRAVNMVMARTKHDIEMYIDAMKELEDRARACYEGPIDLSSNKFSEMLVLDGC 234

Query: 136 FLLDLLQK-----KFLNRPLKFPLDIFYGKLWSEFEIVSDIVKLENQIPLFVLN-----E 195
           F+L+L +        L      P+    G + S   I  D+V LENQ+PLFVLN     +
Sbjct: 235 FVLELFRGADEGFSELGYDRNDPVFAMRGSMHS---IQRDMVMLENQLPLFVLNRLLEIQ 294

Query: 196 ICPKEPINYVAPFLYQFSASVSPFQLPCFNPELECFGFSSYLPEIFNLSHHLLHFL--YS 255
           +  +     V+    +F   + P   P    + +      +   I +     LH L  + 
Sbjct: 295 LGKRHQTGLVSRLAVRFFDPLMPTDEPLTKTD-DSLEQDKFFNPIADKDKGELHCLDVFR 354

Query: 256 LVLVIPVEVPISNRNRTRMNWENSSSSSSSVEFLIECFNILGSVINIAFFQQLKDTFGLI 315
             L+ P   P    +R R +W                                       
Sbjct: 355 RNLLRPCSNPEPRLSRMRWSW--------------------------------------- 414

Query: 316 QRLFRLLGLITKTNSAERKTPLIIPSASDLKTAGVTFKSTKTG-ILKCHFDKTTLTLTLP 375
                      +T  A+++   +I   ++L+ AG+ F++ KT       F    L +   
Sbjct: 415 -----------RTRVADKRQQQLIHCVTELREAGIKFRTRKTDRFWDIRFKNGYLEIPKL 474

Query: 376 SIHLDGFSQVLLKNLVAFEAMAELNPPCFVNYTALMNGLLRNSKDLKILEKAQIVHNHLD 428
            IH DG ++ L  NL+AFE     +     +Y   M+ L+ +S+D++ L    I+ + L 
Sbjct: 475 LIH-DG-TKSLFSNLIAFEQCHIDSSNDITSYIIFMDNLIDSSEDVRYLHYCGIIEHWLG 487

BLAST of Lsi08G000460 vs. TAIR 10
Match: AT3G50120.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 87.4 bits (215), Expect = 3.7e-17
Identity = 103/428 (24.07%), Postives = 178/428 (41.59%), Query Frame = 0

Query: 17  WVHQINDLIDQNALPAASQT--PISVFRLPESVHAGDPTAYLPHHVALGPYHHFRPELYK 76
           WV  I D ++Q      +     + ++R+P  +   D  +Y P  V+LGPYHH +  L  
Sbjct: 80  WVISITDKLEQAHRDDDTTLWGKLCIYRVPYYLQENDNKSYFPQTVSLGPYHHGKKRLRS 139

Query: 77  MDLFKLGKAKNL--KWGPQIHKLADQLKPLELKIRACFDQGHEMNGETLSWVVLIDGLFL 136
           MD  K      +  +    I    D ++ LE K RAC++    ++      ++++DG F+
Sbjct: 140 MDRHKWRAVNRVLKRTNQGIKMYIDAMRELEEKARACYEGPLSLSSNEFIEMLVLDGCFV 199

Query: 137 LDLLQKKF-----LNRPLKFPLDIFYGKLWSEFEIVSDIVKLENQIPLFVLN-----EIC 196
           L+L +        L      P+    G + S   I  D+V LENQ+PLFVLN     ++ 
Sbjct: 200 LELFRGAVEGFTELGYARNDPVFAMRGSMHS---IQRDMVMLENQLPLFVLNRLLELQLG 259

Query: 197 PKEPINYVAPFLYQFSASVSPFQLPCFNPELECFGFSSYLPEIFNLSHHLLHFLYSLVLV 256
            +     VA    +F           F+P                              +
Sbjct: 260 TRNQTGLVAQLAIRF-----------FDP------------------------------L 319

Query: 257 IPVEVPISNRNRTRMNWENSSSSSSSVEFLIECFNILGSVINIAFFQQ--LKDTFGLIQR 316
           +P + P++   ++++  ENS +   S     + F  +G +  +  F++  L+ +     R
Sbjct: 320 MPTDEPLTKSGQSKL--ENSLARDKS----FDPFADMGELHCLDVFRRSLLRSSPKPEPR 379

Query: 317 LFRLLGLITKTNSAERKTPLIIPSASDLKTAGVTFKSTKTG-ILKCHFDKTTLTLTLPSI 376
           L R       T  A+++   +I   ++LK AG+ F+  KT       F    L +    I
Sbjct: 380 LTR-KRWSRNTRVADKRRQQLIHCVTELKEAGIKFRRRKTDRFWDMQFKNGYLEIPRLLI 439

Query: 377 HLDGFSQVLLKNLVAFEAMAELNPPCFVNYTALMNGLLRNSKDLKILEKAQIVHNHLDSE 428
           H DG   + L NL+AFE     +     +Y   M+ L+ + +D+  L    I+ + L S+
Sbjct: 440 H-DGTKSLFL-NLIAFEQCHIDSSNDITSYIIFMDNLIDSHEDVSYLHYCGIIEHWLGSD 454

BLAST of Lsi08G000460 vs. TAIR 10
Match: AT3G47210.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 81.3 bits (199), Expect = 2.7e-15
Identity = 98/404 (24.26%), Postives = 155/404 (38.37%), Query Frame = 0

Query: 30  LPAASQTPISVFRLPESVHAGDPTAYLPHHVALGPYHHFRPELYKMDLFKLGKAKNLKWG 89
           L +A +    +FR+P+S    +P AY P  V++GPYHH R  L  +   KL         
Sbjct: 85  LESAGKESCCIFRVPKSFAEMNPEAYKPKVVSIGPYHHGRKHLEMIQQHKLRFLHLFLRT 144

Query: 90  PQIHK--LADQLKPLELKIRACFDQGHEMNGETLSWVVLIDGLFLLDLLQKKFLNRPLKF 149
             + +  L + +   E +IR  + +G E +   L +++++DG F+L LL        L  
Sbjct: 145 ASVDRDVLFNAVVDWEDEIRKSYSEGLEGSPHELVYMMILDGCFILMLLLIVSRKIELYE 204

Query: 150 PLDIFYGKLWSEFEIVSDIVKLENQIPLFVLNEICPKEPINYVAPFLYQFSASVSPFQLP 209
             D      W    I SD++ LENQ+P FVL  +  K  I  V   L + + S       
Sbjct: 205 SEDPILTIPWILPSIQSDLLLLENQVPFFVLQTLFDKSEIG-VPGDLNRMAFSF------ 264

Query: 210 CFNPELECFGFSSYLPEIFNLSHHLLHFLYSLVLVIPVEVPISNRNRTRMNWENSSSSSS 269
                   F  S   PE + + H   +  + L L+    +P+      ++    S   SS
Sbjct: 265 --------FNLSMDKPERYWVKHRNFNAKHLLDLIRMSFLPMDGYEDFQLTKGKSRKKSS 324

Query: 270 SVEFLIECFNILGSVINIAFFQQLKDTFGLIQRLFRLLGLITKTNSAERKTPLIIPSASD 329
           S                                     GL             ++ SA+ 
Sbjct: 325 S-------------------------------------GL------------TLLLSATR 384

Query: 330 LKTAGVTF--KSTKTGILKCHFDKTTLTLTLPSIHLDGFSQVLLKNLVAFEAMAELNPPC 389
           L   G+ F  +S    +L     K    L +P + LDGF   +L N VAFE     +   
Sbjct: 385 LSLQGIDFSLRSGADSMLDIRLKKN--RLQIPVLRLDGFIISILLNCVAFEQFYAKSTNH 422

Query: 390 FVNYTALMNGLLRNSKDLKILEKAQIVHNHLDSEEEAVELFNGV 430
             +Y   M  LL   +D   L + +I+ N+  SE+E  + F  +
Sbjct: 445 ITSYVVFMGCLLNGKEDATFLSRRRIIENYFGSEKEVSKFFKTI 422

BLAST of Lsi08G000460 vs. TAIR 10
Match: AT5G22550.2 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 77.4 bits (189), Expect = 3.9e-14
Identity = 97/417 (23.26%), Postives = 163/417 (39.09%), Query Frame = 0

Query: 40  VFRLPESVHAGDPTAYLPHHVALGPYHHFRP-------ELYKMDLFKLGKAKNLKWGPQI 99
           ++R+P ++   +  AY P  V++GPYHH          E +K    ++  +K  + G  +
Sbjct: 46  IYRIPHTLKQVNDKAYAPKIVSIGPYHHSSDKQHLKMIEEHKKRYLEMFVSKTKENGVYL 105

Query: 100 HKLADQLKPLELKIRACFDQGHEMNGETLSWVVLIDGLFLLDL---LQKKFLNRPLKFPL 159
             L D +  LE KIR  + +  E + + L  V+L+DG F+L L   + +K     LK P+
Sbjct: 106 IHLVDLVSGLEQKIRDSYSENLEFSQQKLIKVMLLDGCFILMLFLVVSQKIEYTNLKDPI 165

Query: 160 DIFYGKLWSEFEIVSDIVKLENQIPLFVL------NEICPKEPINYVAPFLYQFSASVSP 219
              +   W    + SD++ LENQ+PLF+L      +++ P   +N +A            
Sbjct: 166 ---FKLRWILPTLRSDLLLLENQVPLFLLKVLLETSKLAPSTSLNMLA------------ 225

Query: 220 FQLPCFNPELECFGFSSYLPEIFNLSHHLLHFLYSLVLVIPVEVPISNRNRTRMNWENSS 279
                     + F +S   PE F   H+ L   + L L+    +P    + T        
Sbjct: 226 ---------FKFFDYSIKKPEGFWEKHNNLRAKHLLDLIRKTFIPAPPPSTTPRQC---- 285

Query: 280 SSSSSVEFLIECFNILGSVINIAFFQQLKD-TFGLIQRLFRLLGLITKTNSAERKTPL-- 339
                      C NI       +  +  K+   G I     + G  T +     + P   
Sbjct: 286 -----------CINIFNGPREYSRTETSKNICLGKISCSKEITGAQTSSPPPPPRRPFLG 345

Query: 340 IIPSASDLKTAGVTFKSTKTGILKCHFDKTTLTLTLPSIHLDGFSQVLLKNLVAFEAMAE 399
           +I SA  L+  G+ F   +           +  + +P +  D F   LL N VAFE   +
Sbjct: 346 LIVSARKLRLRGIKFMRKENVETPLDISFKSGLVEIPLLVFDDFISNLLINCVAFE---Q 405

Query: 400 LNPPC---FVNYTALMNGLLRNSKDLKILEKAQIVHNHLDSEEEAVELFNGVENSTS 435
            N  C     ++   M  L+    D   L +  I+ N+  + EE    F  +    S
Sbjct: 406 FNMSCSTEITSFVIFMGCLINTEDDATFLIEKGILENYFGTGEEVSLFFKNIGKDIS 420

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P0C8972.9e-3825.74Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana OX=3702 GN=At3g02645 ... [more]
Q9SD532.5e-1324.88UPF0481 protein At3g47200 OS=Arabidopsis thaliana OX=3702 GN=At3g47200 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KSX99.1e-22976.02Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G650580 PE=4 SV=1[more]
A0A5D3BMY19.4e-22675.05Putative UPF0481 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A1S3CAN64.7e-22574.67putative UPF0481 protein At3g02645 OS=Cucumis melo OX=3656 GN=LOC103498704 PE=4 ... [more]
A0A6J1GBT54.5e-22075.23putative UPF0481 protein At3g02645 OS=Cucurbita moschata OX=3662 GN=LOC111452550... [more]
A0A6J1IBB25.9e-22074.67putative UPF0481 protein At3g02645 OS=Cucurbita maxima OX=3661 GN=LOC111471968 P... [more]
Match NameE-valueIdentityDescription
XP_038890580.12.5e-23378.68putative UPF0481 protein At3g02645 [Benincasa hispida][more]
XP_038890866.12.9e-22976.95putative UPF0481 protein At3g02645 [Benincasa hispida][more]
XP_004141645.11.9e-22876.02putative UPF0481 protein At3g02645 [Cucumis sativus] >KGN52693.1 hypothetical pr... [more]
KAA0039249.11.9e-22575.05putative UPF0481 protein [Cucumis melo var. makuwa] >TYK00435.1 putative UPF0481... [more]
XP_008459642.19.6e-22574.67PREDICTED: putative UPF0481 protein At3g02645 [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT3G02645.12.0e-3925.74Plant protein of unknown function (DUF247) [more]
AT3G50130.15.2e-1923.49Plant protein of unknown function (DUF247) [more]
AT3G50120.13.7e-1724.07Plant protein of unknown function (DUF247) [more]
AT3G47210.12.7e-1524.26Plant protein of unknown function (DUF247) [more]
AT5G22550.23.9e-1423.26Plant protein of unknown function (DUF247) [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004158Protein of unknown function DUF247, plantPFAMPF03140DUF247coord: 40..512
e-value: 3.7E-66
score: 224.1
IPR004158Protein of unknown function DUF247, plantPANTHERPTHR31549PROTEIN, PUTATIVE (DUF247)-RELATED-RELATEDcoord: 16..522
NoneNo IPR availablePANTHERPTHR31549:SF207SUBFAMILY NOT NAMEDcoord: 16..522

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi08G000460.1Lsi08G000460.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane